Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0006775

Physical Location & Seq

Genomic Coordinates Reverse (-)

8649434 .. 8654819

Gene Structure 5386 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT2G20050.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3285 bp

ATGGGTTGTGCTTATTCCAAAACTTGCATTGGTCAGATTTGTGCTACGAAAGAGAATAGTATCAGGCAAACGCATCAACAAGCTCCGTCCAGAGGTGGCACGAGAGCTACTGCGGCGGCGGCGGCGGTTGAGGAGGATAATCCGGTGTTTAATTTCTCTTCAGATGCGGTGGATGATGTAGATAACGACGAAATTCATCAGCTTGGTTTGAGTAGAGACCAGGAATGGGGGATTACTCGGCTTTCTAGGGTTTCTTCTCAATTTCTACCACCAGATGGGTCAAGGGTTGTCAAGGTTCCGTCTTGTAACTATGAATTGAGATGCTCGTTTTTGTCTCAAAGAGGGTATTATCCTGATGCTCTTGATAAGGCCAATCAAGATAGTTTCGCTATTCATACTCCTTTTGGGAGTAATTCGGATGATCATTTCTTTGGGGTTTTTGATGGGCATGGTGAGTTTGGTGCTCAGTGTTCTCAGTTTGTGAAGCGGAGGTTGTGTGAGAATCTTCTTAGACATGGTAGGTTTCGTGTGGATCCTGCTGAGGCTTGTAATTCGGCTTTCTTGACCACGAATTCTCAGTTACATGCTGATTTGGTAGATGATAGTATGAGTGGGACTACTGCGATTACGGTTATGGTTAGAGGAAGGACGATATATGTGGCGAATGCAGGTGATTCCAGGGCGGTTTTAGCTGAGAAGAGGGATGGTGATCTTGTAGCTGTTGATTTGTCTATTGACCAGACTCCTTTTAGACCTGATGAGCTTGAAAGGGTTAAGCTTTGTGGTGCTAGAGTTCTTACTCTTGATCAGATTGAAGGCTTGAAAAATCCTGATGTTCAGTGTTGGGGTACTGAGGAAGATGATGATGGAGACCCTCCGAGACTTTGGGTGCCTAATGGAATGTATCCTGGAACTGCTTTTACCAGGAGTATTGGTGATTCTATTGCAGAGACAATTGGTGTTGTTGCTAACCCTGAGATTGCTGTTGTTGAGCTCACACCGGATAATCCTTTCTTTGTGGTTGCTAGTGATGGCGTATTTGAGTTCATCTCTAGTCAAACGGTGGTTGACATGGTTGCAAAACATAAGGATCCTCGAGATGCTTGTGCTGCAATTGTTGCTGAATCATATAGGCTATGGCTACAGTATGAAACTCGGACAGATGATATAACAATCATCGTCGTGCACATTGATGGACTAAAAGATGATGCTCCTCGGCAATTGTCGAGTACAGGAACTCAATTGCAGCCTCCTATTCCACAAGTTGTGGAGCTTACAGGTTCGGAATCTCCTTCCACCTTTGGATGGAACTCTAAAAATCAACGTGTGAGGCATGATCTATCTCGAGCTAGGATACGTGCCATTGAGAATTCACTAGAGAATGGACATGCTTGGGTTCCTCCATCTCCAGCCCACAGAAAAACCTGGGAAGAAGAAGCACACATTGAGCGGGTGTTGCGTGACCATTTCCTCTTCAGGAAACTCACTGATTCTCAGTGCCAGGTTTTGTTGGACTGCATGCAAAGGCTTGAAGCTAATCCAGGGGATATTGTTGTGAAACAGGGTGGTGAAGGGGACTGCTTCTACGTTGTGGGTAGTGGAGAATTCGAGGTCTTAGCAACTCAGGATGGAAAGAACGGTGAGGTACCTAGGATCTTGCAGCGTTATACAGCTGAGAAACAATCATCATTCGGTGAACTCGCCTTAATGCATAACAAGCCGCTTCAGGCTTCTGTACGTGCTGTAGATCACGGAACATTGTGGGCCTTAAAAAGAGAAGATTTTCGAGGAATTTTGATGTCTGAGTTTTCAAACTTAGCATCCTTGAAGCTTCTTCGTTCTGTCGATCTTCTTTCCCGTCTTACAATTTTGCAACTAAGCCATGTTGCAGAGTCTCTTTCCGAAGCTTGCTTTTCTGATGGACAAACAATTGTTACCAAGGACCAAAAACTTCAGGGCCTGTATGTTATCCAGAAGGGACGCGTAAAAATATCTTTCTGTACAGAGGTGTTGGAGAGTCAAAATGTTTCAAGCCTTACAACTGGAATCACTAACGAGTATGACAATCTTGAAATCGGAACAGAAGTCTCCATAGAAAAGCATGAAGGAAGTTACTTTGGTGAATGGGCTCTTCTTGGCGAACTCAAAGATTCCTTAAGTGTGGTTGCAGTTGGCGAAGTGGTTTGTGTGGTTTTAACAAAAGAAAATTTCGAGTCAGCGGTCGGCCCTCTGACCAATATTTCAGATGACGGTCCCAAGACAAGACATTCTTCATTCGAGCTGTCAAAGGAATCTGCAAAAGTTACCGATACCACAGCTCTTGCTAAAGCCACTCTTGCAGACCTGGAATGGACAACATGCTTGAGTACAACAGACTGTAGTGAGATTGGGCTCGTGCATTTGAAAGATAAAGAAAATTTGCTCAGCTTGAAAAGATTTTCAAAGCAAAAGGTGAAAAAGTTAGGTAAAGAGGCACAAGTATTGAAAGAGCGGAATCTGATGAAGAACGTAATAAAGCCCTCAGCTATTGTTCCCGAAATCTTGTGTACTTGTGTCGATCAAACATTTGCGGCAATCTTACTGAACACTACTCTTGCCTGTCCTATCTCTTCTCTGCTTCACTCTCCGCTTGACGAGTCATCTGTCCGTTTCATTACCGGCTCACTTGTGTCTGCCATAGAAGACATACACAAGAACGAGATTCTCTTCAGAGGTTCATCCCCCGAGTTACTGATGTTGGATCAATCCGGATATCTACAGATTGTAGACTTCAGATTCGCCAAGAAATTGTCCGGGGAACGAACATTTACAATCTGTGGAAATGCAGATTATTTAGCCCCAGAAATTGTTCAAGGCAAAGGCCATGGTTATGCAGCTGACTGGTGGGCTCTTGGAGTTTTGATCTACTATATGCTAGAAGGAGAAATGCCATTCGGGTCATGGAGAGAGAGCGAGCTCGACACGTTTCAGAAGATTGCAAAAGGCCAACTAACTTTCCCTCGAGTTCTAAGCTCAGAAGCTGAAGATCTCATCACAAAGTTGCTTGAAGTTGATGAAAACCTGCGGTTTGGTAGCCAAGGAGGTCCAGAATCAATCAAGAAACATCCCTGGTTCAATGGTCTTAAATGGGAAGCTATTAGTAACCGCGAATTTCAAGTTCCTCAAGAGATAATCTCGCGAATTCATCACCATTTGGAGAATGATAACGTTCTCCCTCTAGAAACTTCTAAATCGCTTGACACAACAGAAGATCAAGATGCTCAAAACTGGCTTGAAGAATGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko04022 ko04540 ko04611 ko04713 ko04714 ko04730 ko04740 ko04923 ko04924 ko04970 map04022 map04540 map04611 map04713 map04714 map04730 map04740 map04923 map04924 map04970

Pfam Domains

Protein Families

PP2C Pkinase cNMP_binding

Protein Analysis

1094

Amino Acids

121.39

Weight (kDa)

5.04

Isoelectric Point (pI)

39.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PP2C	PF00481	132 - 375	6.5e-34	Protein phosphatase 2C
cNMP_binding	PF00027	512 - 598	1.2e-15	Cyclic nucleotide-binding domain
cNMP_binding	PF00027	635 - 737	3.3e-07	Cyclic nucleotide-binding domain
Pkinase	PF00069	795 - 1035	2.1e-36	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	819 - 1019	1.9e-07	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0006775)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G20050 AT2G20050
fragaria_vesca	FvH4_1g05740 FvH4_1g05740 FvH4_1g05740
malus_domestica	MD02G1058500.v1.1 MD15G1192800.v1.1
prunus_persica	Prupe.7G224500_v2.0.a1 Prupe.7G224500_v2.0.a1 Prupe.7G224500_v2.0.a1 Prupe.7G224500_v2.0.a1
pyrus_communis	pycom02g04740 pycom15g17250 pycom15g17260
rosa_chinensis	RchiOBHm_Chr2g0091381
rosa_laevigata	RLG00000016219
rosa_multiflora	Rmu_ssc0000333.1_g000004 Rmu_ssc0000333.1_g000011
rosa_roxburghii	Rroxscaffold_2G00150130
rosa_rugosa	Rorug02G0017200 Rorug02G0017300
rosa_samantha	Rh2AG062300 Rh2BG061300 Rh2CG063400 Rh2DG061900
rosa_wichuraiana	Rw2G005790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	659
Acc36I	ACCTGC	2 cut(s)	659, 3069
Acc65I	GGTACC	1 cut(s)	1645
AccB1I	GGYRCC	2 cut(s)	889, 1645
AccB7I	CCANNNNNTGG	1 cut(s)	275
AccBSI	CCGCTC	2 cut(s)	1450, 2491
AccI	GTMKAC	1 cut(s)	2766
AccII	CGCG	3 cut(s)	1983, 3147, 3178
AccIII	TCCGGA	1 cut(s)	2747
AclI	AACGTT	1 cut(s)	3207
AclWI	GGATC	6 cut(s)	527, 540, 1085, 1098, 1661, 2748
AcsI	RAATTY	9 cut(s)	192, 571, 1369, 1604, 1791, 2206, 2416, 3149, 3180
AcuI	CTGAAG	7 cut(s)	144, 1459, 1709, 1937, 2692, 2755, 3042
AfaI	GTAC	7 cut(s)	850, 1231, 1647, 1737, 2002, 2368, 2548
AfiI	CCNNNNNNNGG	4 cut(s)	92, 226, 275, 1305
AflII	CTTAAG	1 cut(s)	2155
AflIII	ACRYGT	3 cut(s)	1324, 1981, 2961
AjnI	CCWGG	9 cut(s)	219, 677, 907, 923, 1424, 1500, 1540, 2343, 3107
Alw21I	GWGCWC	4 cut(s)	466, 996, 1188, 2958
Alw26I	GTCTC	7 cut(s)	210, 339, 864, 874, 944, 1899, 2092
Alw44I	GTGCAC	1 cut(s)	1184
AlwI	GGATC	6 cut(s)	527, 540, 1085, 1098, 1661, 2748
AlwNI	CAGNNNCTG	2 cut(s)	1960, 3020
Ama87I	CYCGRG	4 cut(s)	1095, 1344, 2723, 3000
Aor13HI	TCCGGA	1 cut(s)	2747
AoxI	GGCC	6 cut(s)	369, 1764, 1957, 2224, 2860, 2983
ApaLI	GTGCAC	1 cut(s)	1184
ApeKI	GCWGC	4 cut(s)	1109, 1246, 1660, 2873
ApoI	RAATTY	9 cut(s)	192, 571, 1369, 1604, 1791, 2206, 2416, 3149, 3180
ArsI	GACNNNNNNTTYG	2 cut(s)	2759, 2791
Asp700I	GAANNNNTTC	2 cut(s)	1782, 2847
Asp718I	GGTACC	1 cut(s)	1645
AspA2I	CCTAGG	1 cut(s)	1649
AspS9I	GGNCC	6 cut(s)	1764, 1942, 1957, 2225, 2252, 3083
AsuC2I	CCSGG	1 cut(s)	2794
AvaI	CYCGRG	4 cut(s)	1095, 1344, 2723, 3000
AvaII	GGWCC	3 cut(s)	1942, 2252, 3083
AvrII	CCTAGG	1 cut(s)	1649
BaeGI	GKGCMC	1 cut(s)	1188
BamHI	GGATCC	2 cut(s)	532, 1090
BanI	GGYRCC	2 cut(s)	889, 1645
BanII	GRGCYC	5 cut(s)	996, 2131, 2394, 2890, 2958
BauI	CACGAG	2 cut(s)	100, 2393
BbsI	GAAGAC	1 cut(s)	2688
Bbv12I	GWGCWC	4 cut(s)	466, 996, 1188, 2958
BbvCI	CCTCAGC	2 cut(s)	540, 2521
BbvI	GCAGC	4 cut(s)	1096, 1258, 1672, 2885
BciT130I	CCWGG	9 cut(s)	221, 679, 909, 925, 1426, 1502, 1542, 2345, 3109
BciVI	GTATCC	2 cut(s)	915, 1347
BclI	TGATCA	2 cut(s)	421, 805
BcnI	CCSGG	1 cut(s)	2794
BcoDI	GTCTC	7 cut(s)	210, 339, 864, 874, 944, 1899, 2092
BfaI	CTAG	9 cut(s)	246, 789, 1026, 1053, 1350, 1376, 1650, 2915, 3218
BfmI	CTRYAG	4 cut(s)	1142, 1743, 2377, 2756
BfrI	CTTAAG	1 cut(s)	2155
BfuAI	ACCTGC	2 cut(s)	659, 3069
BfuI	GTATCC	2 cut(s)	915, 1347
BglII	AGATCT	1 cut(s)	3025
BlnI	CCTAGG	1 cut(s)	1649
BlpI	GCTNAGC	1 cut(s)	2423
Bme18I	GGWCC	3 cut(s)	1942, 2252, 3083
BmeT110I	CYCGRG	4 cut(s)	1095, 1344, 2723, 3000
BmgT120I	GGNCC	6 cut(s)	1764, 1942, 1957, 2225, 2252, 3083
BmiI	GGNNCC	7 cut(s)	297, 534, 891, 1092, 1398, 1647, 2254
BmsI	GCATC	8 cut(s)	82, 154, 311, 346, 1090, 1198, 1829, 3247
BpiI	GAAGAC	1 cut(s)	2688
BplI	GAGNNNNNCTC	4 cut(s)	1034, 1066, 3158, 3190
BpmI	CTGGAG	1 cut(s)	1392
Bpu10I	CCTNAGC	2 cut(s)	540, 2521
Bpu1102I	GCTNAGC	1 cut(s)	2423
BpuEI	CTTGAG	2 cut(s)	2383, 3147
BpuMI	CCSGG	1 cut(s)	2794
BsaAI	YACGTR	2 cut(s)	1358, 1739
BsaBI	GATNNNNATC	1 cut(s)	708
BsaI	GGTCTC	2 cut(s)	210, 864
BsaWI	WCCGGW	3 cut(s)	142, 1000, 2747
BsaXI	ACNNNNNCTCC	4 cut(s)	481, 511, 919, 949
Bsc4I	CCNNNNNNNGG	4 cut(s)	92, 226, 275, 1305
Bse118I	RCCGGY	1 cut(s)	2657
Bse1I	ACTGG	3 cut(s)	2047, 2885, 3272
Bse8I	GATNNNNATC	1 cut(s)	708
BseAI	TCCGGA	1 cut(s)	2747
BseBI	CCWGG	9 cut(s)	221, 679, 909, 925, 1426, 1502, 1542, 2345, 3109
BseGI	GGATG	8 cut(s)	178, 424, 709, 1310, 1633, 1820, 2717, 3103
BseJI	GATNNNNATC	1 cut(s)	708
BseLI	CCNNNNNNNGG	4 cut(s)	92, 226, 275, 1305
BseNI	ACTGG	3 cut(s)	2047, 2885, 3272
BseRI	GAGGAG	2 cut(s)	146, 1203
BseSI	GKGCMC	1 cut(s)	1188
BseXI	GCAGC	4 cut(s)	1096, 1258, 1672, 2885
Bsh1236I	CGCG	3 cut(s)	1983, 3147, 3178
Bsh1285I	CGRYCG	1 cut(s)	2223
BshFI	GGCC	6 cut(s)	371, 1766, 1959, 2226, 2862, 2985
BshNI	GGYRCC	2 cut(s)	889, 1645
BsiEI	CGRYCG	1 cut(s)	2223
BsiHKAI	GWGCWC	4 cut(s)	466, 996, 1188, 2958
BsiHKCI	CYCGRG	4 cut(s)	1095, 1344, 2723, 3000
BsiSI	CCGG	5 cut(s)	143, 1001, 2658, 2748, 2793
BslFI	GGGAC	4 cut(s)	628, 1589, 1992, 2238
BslI	CCNNNNNNNGG	4 cut(s)	92, 226, 275, 1305
BsmAI	GTCTC	7 cut(s)	210, 339, 864, 874, 944, 1899, 2092
BsmFI	GGGAC	4 cut(s)	628, 1589, 1992, 2238
BsmI	GAATGC	1 cut(s)	670
BsnI	GGCC	6 cut(s)	371, 1766, 1959, 2226, 2862, 2985
Bso31I	GGTCTC	2 cut(s)	210, 864
BsoBI	CYCGRG	4 cut(s)	1095, 1344, 2723, 3000
Bsp1286I	GDGCHC	7 cut(s)	466, 996, 1188, 2131, 2394, 2890, 2958
Bsp13I	TCCGGA	1 cut(s)	2747
Bsp1407I	TGTACA	1 cut(s)	2000
Bsp1720I	GCTNAGC	1 cut(s)	2423
Bsp19I	CCATGG	1 cut(s)	2863
Bsp68I	TCGCGA	1 cut(s)	3178
BspANI	GGCC	6 cut(s)	371, 1766, 1959, 2226, 2862, 2985
BspEI	TCCGGA	1 cut(s)	2747
BspFNI	CGCG	3 cut(s)	1983, 3147, 3178
BspLI	GGNNCC	7 cut(s)	297, 534, 891, 1092, 1398, 1647, 2254
BspMI	ACCTGC	2 cut(s)	659, 3069
BspPI	GGATC	6 cut(s)	527, 540, 1085, 1098, 1661, 2748
BspQI	GCTCTTC	1 cut(s)	2136
BspT107I	GGYRCC	2 cut(s)	889, 1645
BspTI	CTTAAG	1 cut(s)	2155
BspTNI	GGTCTC	2 cut(s)	210, 864
BsrBI	CCGCTC	2 cut(s)	1450, 2491
BsrFI	RCCGGY	1 cut(s)	2657
BsrGI	TGTACA	1 cut(s)	2000
BsrI	ACTGG	3 cut(s)	2047, 2885, 3272
BssAI	RCCGGY	1 cut(s)	2657
BssSI	CACGAG	2 cut(s)	100, 2393
BssT1I	CCWWGG	4 cut(s)	1649, 1938, 2863, 3076
Bst2BI	CACGAG	2 cut(s)	100, 2393
Bst2UI	CCWGG	9 cut(s)	221, 679, 909, 925, 1426, 1502, 1542, 2345, 3109
Bst4CI	ACNGT	6 cut(s)	631, 1063, 1146, 1640, 2252, 2378
Bst6I	CTCTTC	6 cut(s)	163, 692, 1478, 2136, 2614, 2711
BstAFI	CTTAAG	1 cut(s)	2155
BstAPI	GCANNNNNTGC	1 cut(s)	1118
BstAUI	TGTACA	1 cut(s)	2000
BstBAI	YACGTR	2 cut(s)	1358, 1739
BstC8I	GCNNGC	3 cut(s)	1520, 1909, 2954
BstDSI	CCRYGG	1 cut(s)	2863
BstF5I	GGATG	8 cut(s)	178, 424, 709, 1310, 1633, 1820, 2717, 3103
BstFNI	CGCG	3 cut(s)	1983, 3147, 3178
BstMAI	GTCTC	7 cut(s)	210, 339, 864, 874, 944, 1899, 2092
BstMCI	CGRYCG	1 cut(s)	2223
BstMWI	GCNNNNNNNGC	9 cut(s)	113, 119, 122, 689, 1109, 1118, 1456, 1717, 2336
BstNI	CCWGG	9 cut(s)	221, 679, 909, 925, 1426, 1502, 1542, 2345, 3109
BstNSI	RCATGY	4 cut(s)	587, 1391, 1522, 2361
BstSFI	CTRYAG	4 cut(s)	1142, 1743, 2377, 2756
BstSLI	GKGCMC	1 cut(s)	1188
BstUI	CGCG	3 cut(s)	1983, 3147, 3178
BstV1I	GCAGC	4 cut(s)	1096, 1258, 1672, 2885
BstV2I	GAAGAC	1 cut(s)	2688
BstX2I	RGATCY	4 cut(s)	532, 1090, 1653, 3025
BstYI	RGATCY	4 cut(s)	532, 1090, 1653, 3025
BsuI	GTATCC	2 cut(s)	915, 1347
BsuRI	GGCC	6 cut(s)	371, 1766, 1959, 2226, 2862, 2985
BtgI	CCRYGG	1 cut(s)	2863
BtsCI	GGATG	8 cut(s)	178, 424, 709, 1310, 1633, 1820, 2717, 3103
BtsIMutI	CAGTG	4 cut(s)	473, 845, 1485, 1502
BtuMI	TCGCGA	1 cut(s)	3178
BveI	ACCTGC	2 cut(s)	659, 3069
Cac8I	GCNNGC	3 cut(s)	1520, 1909, 2954
CaiI	CAGNNNCTG	2 cut(s)	1960, 3020
Cfr10I	RCCGGY	1 cut(s)	2657
Cfr13I	GGNCC	6 cut(s)	1764, 1942, 1957, 2225, 2252, 3083
CseI	GACGC	1 cut(s)	1989
Csp6I	GTAC	7 cut(s)	849, 1230, 1646, 1736, 2001, 2367, 2547
CspCI	CAANNNNNGTGG	2 cut(s)	2302, 2337
CviQI	GTAC	7 cut(s)	849, 1230, 1646, 1736, 2001, 2367, 2547
Eam1104I	CTCTTC	6 cut(s)	163, 692, 1478, 2136, 2614, 2711
EarI	CTCTTC	6 cut(s)	163, 692, 1478, 2136, 2614, 2711
Ecl136II	GAGCTC	2 cut(s)	994, 2956
Eco130I	CCWWGG	4 cut(s)	1649, 1938, 2863, 3076
Eco24I	GRGCYC	5 cut(s)	996, 2131, 2394, 2890, 2958
Eco31I	GGTCTC	2 cut(s)	210, 864
Eco32I	GATATC	1 cut(s)	2753
Eco47I	GGWCC	3 cut(s)	1942, 2252, 3083
Eco53kI	GAGCTC	2 cut(s)	994, 2956
Eco57I	CTGAAG	7 cut(s)	144, 1459, 1709, 1937, 2692, 2755, 3042
Eco88I	CYCGRG	4 cut(s)	1095, 1344, 2723, 3000
EcoICRI	GAGCTC	2 cut(s)	994, 2956
EcoO109I	RGGNCCY	1 cut(s)	1957
EcoRI	GAATTC	4 cut(s)	571, 1369, 1604, 3180
EcoRII	CCWGG	9 cut(s)	219, 677, 907, 923, 1424, 1500, 1540, 2343, 3107
EcoRV	GATATC	1 cut(s)	2753
EcoT14I	CCWWGG	4 cut(s)	1649, 1938, 2863, 3076
EcoT22I	ATGCAT	1 cut(s)	1713
EcoT38I	GRGCYC	5 cut(s)	996, 2131, 2394, 2890, 2958
ErhI	CCWWGG	4 cut(s)	1649, 1938, 2863, 3076
FalI	AAGNNNNNCTT	4 cut(s)	2254, 2286, 2319, 2351
FaqI	GGGAC	4 cut(s)	628, 1589, 1992, 2238
FauI	CCCGC	1 cut(s)	1443
FbaI	TGATCA	2 cut(s)	421, 805
FblI	GTMKAC	1 cut(s)	2766
FokI	GGATG	8 cut(s)	185, 431, 716, 1317, 1640, 1807, 2704, 3090
FriOI	GRGCYC	5 cut(s)	996, 2131, 2394, 2890, 2958
FspBI	CTAG	9 cut(s)	246, 789, 1026, 1053, 1350, 1376, 1650, 2915, 3218
GsuI	CTGGAG	1 cut(s)	1392
HaeIII	GGCC	6 cut(s)	371, 1766, 1959, 2226, 2862, 2985
HapII	CCGG	5 cut(s)	143, 1001, 2658, 2748, 2793
HgaI	GACGC	1 cut(s)	1989
HincII	GTYRAC	1 cut(s)	1069
HindII	GTYRAC	1 cut(s)	1069
HindIII	AAGCTT	3 cut(s)	776, 1829, 1905
HpaII	CCGG	5 cut(s)	143, 1001, 2658, 2748, 2793
Hpy166II	GTNNAC	5 cut(s)	1069, 1186, 1697, 2547, 2767
Hpy8I	GTNNAC	5 cut(s)	1069, 1186, 1697, 2547, 2767
Hpy99I	CGWCG	2 cut(s)	191, 1184
HpyAV	CCTTC	7 cut(s)	639, 809, 1302, 1565, 1969, 2099, 2912
HpyCH4III	ACNGT	6 cut(s)	631, 1063, 1146, 1640, 2252, 2378
HpyCH4IV	ACGT	7 cut(s)	1324, 1357, 1587, 1738, 2508, 2963, 3207
HpyF10VI	GCNNNNNNNGC	9 cut(s)	113, 119, 122, 689, 1109, 1118, 1456, 1717, 2336
HpySE526I	ACGT	7 cut(s)	1324, 1357, 1587, 1738, 2508, 2963, 3207
Kpn2I	TCCGGA	1 cut(s)	2747
KpnI	GGTACC	1 cut(s)	1649
Ksp22I	TGATCA	2 cut(s)	421, 805
LguI	GCTCTTC	1 cut(s)	2136
LmnI	GCTCC	3 cut(s)	88, 1216, 1270
Lsp1109I	GCAGC	4 cut(s)	1096, 1258, 1672, 2885
LweI	GCATC	8 cut(s)	82, 154, 311, 346, 1090, 1198, 1829, 3247
MaeI	CTAG	9 cut(s)	246, 789, 1026, 1053, 1350, 1376, 1650, 2915, 3218
MaeII	ACGT	7 cut(s)	1324, 1357, 1587, 1738, 2508, 2963, 3207
MaeIII	GTNAC	8 cut(s)	305, 579, 1460, 1933, 2111, 2302, 2727, 3140
MbiI	CCGCTC	2 cut(s)	1450, 2491
MfeI	CAATTG	5 cut(s)	954, 1113, 1220, 1241, 1929
MflI	RGATCY	4 cut(s)	532, 1090, 1653, 3025
MhlI	GDGCHC	7 cut(s)	466, 996, 1188, 2131, 2394, 2890, 2958
MluI	ACGCGT	1 cut(s)	1981
MlyI	GAGTC	5 cut(s)	736, 1901, 2026, 2222, 2645
MmeI	TCCRAC	3 cut(s)	1491, 1992, 2718
Mph1103I	ATGCAT	1 cut(s)	1713
MroI	TCCGGA	1 cut(s)	2747
MroXI	GAANNNNTTC	2 cut(s)	1782, 2847
MseI	TTAA	7 cut(s)	150, 774, 1706, 1769, 2156, 2195, 3123
MslI	CAYNNNNRTG	3 cut(s)	1181, 1191, 2868
MspA1I	CMGCKG	3 cut(s)	1673, 2219, 2876
MspCI	CTTAAG	1 cut(s)	2155
MspI	CCGG	5 cut(s)	143, 1001, 2658, 2748, 2793
MunI	CAATTG	5 cut(s)	954, 1113, 1220, 1241, 1929
Mva1269I	GAATGC	1 cut(s)	670
MvaI	CCWGG	9 cut(s)	221, 679, 909, 925, 1426, 1502, 1542, 2345, 3109
MvnI	CGCG	3 cut(s)	1983, 3147, 3178
MwoI	GCNNNNNNNGC	9 cut(s)	113, 119, 122, 689, 1109, 1118, 1456, 1717, 2336
NciI	CCSGG	1 cut(s)	2794
NcoI	CCATGG	1 cut(s)	2863
NlaIV	GGNNCC	7 cut(s)	297, 534, 891, 1092, 1398, 1647, 2254
NmeAIII	GCCGAG	2 cut(s)	217, 1195
NmuCI	GTSAC	1 cut(s)	1460
NruI	TCGCGA	1 cut(s)	3178
NsiI	ATGCAT	1 cut(s)	1713
NspI	RCATGY	4 cut(s)	587, 1391, 1522, 2361
PaeI	GCATGC	1 cut(s)	1522
PaeR7I	CTCGAG	3 cut(s)	1095, 1344, 3000
PaqCI	CACCTGC	1 cut(s)	659
PciSI	GCTCTTC	1 cut(s)	2136
PctI	GAATGC	1 cut(s)	670
PdmI	GAANNNNTTC	2 cut(s)	1782, 2847
PflMI	CCANNNNNTGG	1 cut(s)	275
PfoI	TCCNGGA	1 cut(s)	907
PleI	GAGTC	5 cut(s)	736, 1900, 2025, 2221, 2644
PpsI	GAGTC	5 cut(s)	736, 1900, 2025, 2221, 2644
Ppu21I	YACGTR	2 cut(s)	1358, 1739
Psp124BI	GAGCTC	2 cut(s)	996, 2958
Psp1406I	AACGTT	1 cut(s)	3207
Psp6I	CCWGG	9 cut(s)	219, 677, 907, 923, 1424, 1500, 1540, 2343, 3107
PspGI	CCWGG	9 cut(s)	219, 677, 907, 923, 1424, 1500, 1540, 2343, 3107
PspN4I	GGNNCC	7 cut(s)	297, 534, 891, 1092, 1398, 1647, 2254
PspPI	GGNCC	6 cut(s)	1764, 1942, 1957, 2225, 2252, 3083
PspXI	VCTCGAGB	1 cut(s)	3000
PsrI	GAACNNNNNNTAC	2 cut(s)	904, 936
PstNI	CAGNNNCTG	2 cut(s)	1960, 3020
PsuI	RGATCY	4 cut(s)	532, 1090, 1653, 3025
PvuII	CAGCTG	2 cut(s)	1673, 2876
RruI	TCGCGA	1 cut(s)	3178
RsaI	GTAC	7 cut(s)	850, 1231, 1647, 1737, 2002, 2368, 2548
RsaNI	GTAC	7 cut(s)	849, 1230, 1646, 1736, 2001, 2367, 2547
RseI	CAYNNNNRTG	3 cut(s)	1181, 1191, 2868
SacI	GAGCTC	2 cut(s)	996, 2958
SapI	GCTCTTC	1 cut(s)	2136
SaqAI	TTAA	7 cut(s)	150, 774, 1706, 1769, 2156, 2195, 3123
Sau96I	GGNCC	6 cut(s)	1764, 1942, 1957, 2225, 2252, 3083
SchI	GAGTC	5 cut(s)	736, 1901, 2026, 2222, 2645
SduI	GDGCHC	7 cut(s)	466, 996, 1188, 2131, 2394, 2890, 2958
SfaNI	GCATC	8 cut(s)	82, 154, 311, 346, 1090, 1198, 1829, 3247
SfcI	CTRYAG	4 cut(s)	1142, 1743, 2377, 2756
Sfr274I	CTCGAG	3 cut(s)	1095, 1344, 3000
SinI	GGWCC	3 cut(s)	1942, 2252, 3083
SlaI	CTCGAG	3 cut(s)	1095, 1344, 3000
SmiMI	CAYNNNNRTG	3 cut(s)	1181, 1191, 2868
SmlI	CTYRAG	6 cut(s)	1095, 1344, 2155, 2362, 3000, 3162
SmoI	CTYRAG	6 cut(s)	1095, 1344, 2155, 2362, 3000, 3162
SphI	GCATGC	1 cut(s)	1522
SspI	AATATT	1 cut(s)	2239
SspMI	CTAG	9 cut(s)	246, 789, 1026, 1053, 1350, 1376, 1650, 2915, 3218
SstI	GAGCTC	2 cut(s)	996, 2958
StyI	CCWWGG	4 cut(s)	1649, 1938, 2863, 3076
TaaI	ACNGT	6 cut(s)	631, 1063, 1146, 1640, 2252, 2378
TaiI	ACGT	7 cut(s)	1327, 1360, 1590, 1741, 2511, 2966, 3210
TatI	WGTACW	4 cut(s)	1229, 2000, 2366, 2546
TauI	GCSGC	6 cut(s)	116, 119, 122, 125, 1723, 2573
Tru1I	TTAA	7 cut(s)	150, 774, 1706, 1769, 2156, 2195, 3123
Tru9I	TTAA	7 cut(s)	150, 774, 1706, 1769, 2156, 2195, 3123
TscAI	CASTG	4 cut(s)	473, 845, 1492, 1502
TseFI	GTSAC	1 cut(s)	1460
TseI	GCWGC	4 cut(s)	1109, 1246, 1660, 2873
Tsp45I	GTSAC	1 cut(s)	1460
TspGWI	ACGGA	4 cut(s)	75, 288, 1767, 2636
TspRI	CASTG	4 cut(s)	473, 845, 1492, 1502
Van91I	CCANNNNNTGG	1 cut(s)	275
Vha464I	CTTAAG	1 cut(s)	2155
VneI	GTGCAC	1 cut(s)	1184
VpaK11BI	GGWCC	3 cut(s)	1942, 2252, 3083
XapI	RAATTY	9 cut(s)	192, 571, 1369, 1604, 1791, 2206, 2416, 3149, 3180
XbaI	TCTAGA	1 cut(s)	3217
XceI	RCATGY	4 cut(s)	587, 1391, 1522, 2361
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2937
XhoI	CTCGAG	3 cut(s)	1095, 1344, 3000
XmaJI	CCTAGG	1 cut(s)	1649
XmiI	GTMKAC	1 cut(s)	2766
XmnI	GAANNNNTTC	2 cut(s)	1782, 2847
XspI	CTAG	9 cut(s)	246, 789, 1026, 1053, 1350, 1376, 1650, 2915, 3218
Zsp2I	ATGCAT	1 cut(s)	1713

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.