Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0006775

Physical Location & Seq

Genomic Coordinates Forward (+)

4695795 .. 4702267

Gene Structure 6473 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1058500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3243 bp

ATGATGGGTTGTGTGTATTCGAGAGTTTGCATTGGAGAGGTTTGTTCTCCGAGAGTTCCGAAATTGAAAGAGAATCAGGATGTGAGGAGCACTGAGATCCCAGTGTTCTCTCCTACTTCCTCCAATGGAGAAGTAGGTGAGCTTCAAGACCAGTTCAACCAGTCTGACTTAACTGGGGATGCTGAGGTGGGTATCACTAGGCTCCGTAGGGTTTCATCACAGTTTCTGCCTCCCAATGGATCAAGAACTGTGAAGGTTCCTTCGGGAAACTTCGAAATGCGGTATTCTTTTTTGTCTCAGAGAGGTTATTACCCTGATGCTCTTGATAAGGCTAACCAAGATAGCTTTTGCATCCACACGCCATTCGGGACAAATCCGGATGACCATTTCTTTGGGGTCTTTGATGGTCATGGAGAATTCGGAGCTGAGTGTTCGCAGTTTGTGAAAAGAAAGTTGTGTGAGAATTTGCTTAGGAATAATAAGTTTCAAGTGGATGCTGTCGAGGCGTGCCATTCTGCGTTTATTGCAACCAATTCTCAGTTGCACGCTGATAGTTTGGATGATAGCATGAGCGGGACAACTGCAATTACGGTTTTGGTTCGAGGTAGGACAATATGCATTGCAAATTCGGGTGATTCAAGGGCCGTTATGGCAGAGAGGAAAGGGGATGACATCGTGGCTGTGGATCTTTCAATTGATCAAACCCCATTTAGAGTGGACGAGCTCGAAAGGGTTAAGCTTTGTGGTGCAAGAGTTCTTACGTTGGATCAGATTGAAGGGCTGAAGAATCCAGATGTGCAGTGTTGGGGAACCGAAGAAGGCGATGATGGTGATCCACCTAGATTGTGGGTGCCAAACGGAATGTATCCTGGAACAGCTTTTACGAGGAGTATTGGTGATTCGACTGCTGAGACAATTGGGGTTGTTGCCAACCCTGAAATCGTGGTTTTAGAGCTTACACAGAATAATCCTTTCTTTGTTCTTGCTAGTGATGGAGTTTTTGAGTTCCTTTCTAGCCAAGCTGTGGTGGATATGGTTGCAAAATTTAAAGATCCCCGTGATGCTTGTGCTGCAATTGTAGCTGAATCATATAAACTCTGGCTGCAGTATGAAACTCGTACAGATGATATTACAGTGATCGTTGTGCATGTAAATGGGCTGACTGATACGTCCATCCGTCAATCAGTAAGCCCTGCTGCTGCTTTACGACCAACTGTTCCTCAAGTTGTAGAGGTTACAGGATGTGAATCTCCTTCACCCACTGGGTGGAACTCTAGCAACCAGCGCACCAGACATGATTTGTCAAGGGCACGCCTTCGTGTGATTGAGAGTTCTCTAGAAAATGGGCAAATTTGGGTTCCTCCACCGCCATCCCACAGAAAGACTTGGGAGGAAGAAGCACACATTCAGCGGGGTTTGCACGACCATTTCCTCTTCAGAAAGCTTACTGATTCTCAGTGTCAGGTGTTATTGGATTGCATGGAAAGGGTTGAGTTCCAGCCTGGAGATGTTGTTGTTAGACAGGGTGGTGAAGGTGACTGCTTTTATGTTGTTGGCAGTGGAGAATTTGAGGTGTTGGCAACCCAGGAAGAAAAGAATGGAGAGGTTCCAAGGGTTCTGCAGCGCTATACAGCTGATAAGCTGTCATCCTTTGGGGAACTAGCGCTAATGCATAACAAACCACTACAGGCCTCCGTTCGAGCTGTGACCAGTGGAACTCTTTGGGCTTTGAAAAGAGAAGACTTCCGTGGAATTTTAACATCAGAGTTTTCTAATTTGTCATATTTGAAGTTGCTTCGATCAGTGGATCTTCTATCGAGGCTGACAATCTTACAGCTGAGCCATATCGCCGATTCTCTGTCTGAAGTTTCCTTTTCAGAAGGCCAGACAATAGTCAGTAAGAGTGAAGACCTAGTTGGATTATACATTATCCAGAAGGGAAAAGTGAGGATTACTTTTGATGCAATTTCAGCTAGTAGTTCAGTTGTCCGGAGTCTCAAGTCTGACTATCAAAAAGAAGATGATTATCCTCAGAGCAGTAAAGAACTCTCAGTGGAGAAGACGGAGGGAAGCTGCTTTGGTGAATGGGCACTTCTTGGAGAACATATTGATTTGTTTAGAGCAGTTGCTGTGGGAGATGTCACCTGTGCTGTTTTAACGAAGGAAAATTTTGATTCAGCCGTTGGCCCTTTGACAAAGATTTCTCAGGATGATCAGAAGTCAAGGGATTATTCTTCAGAAGTTCCAAAGGAATCTGCCAAGAATATTGATATGTCTGCTCTTACTGAAGTCCAGTTGTCTGATCTAGAATGGAGAACAATTTTGTATAGCACTGACTGCAGTGAAATTGGAGTTGTTCGTTTAAGAGATTCAGAAAAATTGCTCAGTTTGAAAAGGTTTTCAAAGCAAATGGTCAGAAGGATGGGAAAGGAAGCGCAAGTGTTAAAAGAGAAGGATCTAATCAAGAGTATGAGCTCTTCAGCGTGTGTGCCACAGTTTCTGTGCACATGTGTTGATCAGACACATGCTGGCATACTGTTTAATACTTGCCTTGCTTGTCCGTTGGCTTCGATACTTCACACTCCCCTTGATGAACCGTCTGCCAAATTTTGTGCAGCCTCTCTAGTTGCTGGGTTGGCAGATCTCCACAAGAATGACGTTCTCTACAGAGGGTTGTCTCATGATGTTTTAATGTTGGACCAAACAGGATATTTACAGCTAGTGGACTTCAAGTTTGGGAAGAAGTTATCCGGCGAAAGAACATATACAATTTGTGGCATGGTGGACTTTTTAGCTCCAGAGGTAGTCCAGGGAAAAGGCCATGGTTTTCCATCTGACTGGTGGGCATTAGGAGTGTTGATATACTTCATGCTACGGGGTGAAATGCCATTCGGCTCATGGAGACAAAGTGAGCTTGATACATTTGCAAAGATCGCAAAGGGACAACTAACTCTACCGCAAACTTTTAGTCCTGAAGTTGTCGATCTCATCACTAAGTTACTCGAGGTTGATGAAAACACCAGACTAGGAAGCCAAGGTTATGAATCTGTCAAAAGGCATCCCTGGTTTGATGGCACTGATTGGGAAGGGATCAAAGACCGTAGTTTGCCCGTTCCTCATGAGATCACCTCTCGTATAACTCAACATTTGGGAAGTCACTCCGAGGACTGTTCTGTTCCTCTAGCTTCTCCATCTCGAAATGGAGAAGAACTCGAAACTCCTGAATGGTTTGACGACTGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko04022 ko04540 ko04611 ko04713 ko04714 ko04730 ko04740 ko04923 ko04924 ko04970 map04022 map04540 map04611 map04713 map04714 map04730 map04740 map04923 map04924 map04970

Pfam Domains

Protein Families

PP2C Pkinase cNMP_binding

Protein Analysis

1081

Amino Acids

119.87

Weight (kDa)

5.06

Isoelectric Point (pI)

42.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PP2C	PF00481	120 - 362	1.7e-32	Protein phosphatase 2C
cNMP_binding	PF00027	497 - 587	2.4e-16	Cyclic nucleotide-binding domain
Pkinase	PF00069	787 - 1023	3.7e-36	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	810 - 1008	1.5e-12	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0006775)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G20050 AT2G20050
fragaria_vesca	FvH4_1g05740 FvH4_1g05740 FvH4_1g05740
malus_domestica	MD02G1058500.v1.1 MD15G1192800.v1.1
prunus_persica	Prupe.7G224500_v2.0.a1 Prupe.7G224500_v2.0.a1 Prupe.7G224500_v2.0.a1 Prupe.7G224500_v2.0.a1
pyrus_communis	pycom02g04740 pycom15g17250 pycom15g17260
rosa_chinensis	RchiOBHm_Chr2g0091381
rosa_laevigata	RLG00000016219
rosa_multiflora	Rmu_ssc0000333.1_g000004 Rmu_ssc0000333.1_g000011
rosa_roxburghii	Rroxscaffold_2G00150130
rosa_rugosa	Rorug02G0017200 Rorug02G0017300
rosa_samantha	Rh2AG062300 Rh2BG061300 Rh2CG063400 Rh2DG061900
rosa_wichuraiana	Rw2G005790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1168
AccB1I	GGYRCC	1 cut(s)	850
AccB7I	CCANNNNNTGG	2 cut(s)	1024, 1266
AccBSI	CCGCTC	1 cut(s)	573
AccIII	TCCGGA	2 cut(s)	376, 1989
AciI	CCGC	5 cut(s)	280, 573, 1367, 1411, 2957
AclWI	GGATC	9 cut(s)	91, 247, 693, 774, 827, 1046, 1815, 2463, 3098
AcsI	RAATTY	9 cut(s)	416, 463, 625, 1043, 1350, 1565, 1752, 2167, 2606
AcuI	CTGAAG	7 cut(s)	803, 1420, 1884, 2220, 2307, 2463, 2994
AdeI	CACNNNGTG	1 cut(s)	1266
AfaI	GTAC	1 cut(s)	1120
AfeI	AGCGCT	2 cut(s)	1625, 1665
AfiI	CCNNNNNNNGG	3 cut(s)	236, 1024, 1266
AflIII	ACRYGT	1 cut(s)	2507
AjnI	CCWGG	5 cut(s)	868, 1501, 1584, 2808, 3062
AjuI	GAANNNNNNNTTGG	2 cut(s)	1899, 1931
Alw21I	GWGCWC	4 cut(s)	92, 726, 2477, 2507
Alw26I	GTCTC	5 cut(s)	300, 905, 2000, 2682, 2896
Alw44I	GTGCAC	1 cut(s)	2503
AlwI	GGATC	9 cut(s)	91, 247, 693, 774, 827, 1046, 1815, 2463, 3098
AlwNI	CAGNNNCTG	3 cut(s)	226, 2129, 2500
Ama87I	CYCGRG	1 cut(s)	3002
Aor13HI	TCCGGA	2 cut(s)	376, 1989
Aor51HI	AGCGCT	2 cut(s)	1625, 1665
AoxI	GGCC	5 cut(s)	642, 1689, 1882, 2185, 2818
ApaLI	GTGCAC	1 cut(s)	2503
ApeKI	GCWGC	7 cut(s)	1070, 1102, 1196, 1199, 1621, 2073, 2615
ApoI	RAATTY	9 cut(s)	416, 463, 625, 1043, 1350, 1565, 1752, 2167, 2606
ArsI	GACNNNNNNTTYG	6 cut(s)	374, 406, 2717, 2749, 2953, 2985
AspLEI	GCGC	4 cut(s)	1287, 1626, 1666, 2437
AspS9I	GGNCC	3 cut(s)	642, 2186, 2698
AsuII	TTCGAA	1 cut(s)	273
AvaI	CYCGRG	1 cut(s)	3002
AvaII	GGWCC	1 cut(s)	2698
BaeGI	GKGCMC	3 cut(s)	1312, 2092, 2507
BanI	GGYRCC	1 cut(s)	850
BanII	GRGCYC	2 cut(s)	726, 2477
BarI	GAAGNNNNNNTAC	4 cut(s)	126, 158, 2461, 2493
BbsI	GAAGAC	3 cut(s)	1746, 1914, 2066
Bbv12I	GWGCWC	4 cut(s)	92, 726, 2477, 2507
BbvCI	CCTCAGC	1 cut(s)	183
BbvI	GCAGC	7 cut(s)	1057, 1089, 1183, 1186, 1633, 2060, 2627
BccI	CCATC	9 cut(s)	398, 821, 986, 1181, 1378, 2416, 2839, 3065, 3199
BceAI	ACGGC	2 cut(s)	629, 2165
BciT130I	CCWGG	5 cut(s)	870, 1503, 1586, 2810, 3064
BciVI	GTATCC	1 cut(s)	876
BclI	TGATCA	3 cut(s)	697, 2212, 2515
BcoDI	GTCTC	5 cut(s)	300, 905, 2000, 2682, 2896
BfmI	CTRYAG	5 cut(s)	1103, 1619, 1685, 2338, 2665
BfoI	RGCGCY	2 cut(s)	1627, 1667
BfuI	GTATCC	1 cut(s)	876
BglI	GCCNNNNNGGC	1 cut(s)	650
BglII	AGATCT	1 cut(s)	2641
BisI	GCNGC	7 cut(s)	1071, 1103, 1197, 1200, 1622, 2074, 2616
BlpI	GCTNAGC	1 cut(s)	1838
BlsI	GCNGC	7 cut(s)	1072, 1104, 1198, 1201, 1623, 2075, 2617
Bme1390I	CCNGG	5 cut(s)	870, 1503, 1586, 2810, 3064
Bme18I	GGWCC	1 cut(s)	2698
BmeT110I	CYCGRG	1 cut(s)	3002
BmgT120I	GGNCC	3 cut(s)	642, 2186, 2698
BmiI	GGNNCC	6 cut(s)	203, 258, 811, 852, 1359, 1608
BmrFI	CCNGG	5 cut(s)	870, 1503, 1586, 2810, 3064
BmrI	ACTGGG	3 cut(s)	95, 183, 1272
BmsI	GCATC	7 cut(s)	169, 307, 360, 484, 1051, 1951, 3067
BmuI	ACTGGG	3 cut(s)	95, 183, 1272
BoxI	GACNNNNGTC	1 cut(s)	1892
BpiI	GAAGAC	3 cut(s)	1746, 1914, 2066
BplI	GAGNNNNNCTC	4 cut(s)	3113, 3145, 3195, 3227
BpmI	CTGGAG	2 cut(s)	1524, 2781
Bpu10I	CCTNAGC	2 cut(s)	183, 470
Bpu1102I	GCTNAGC	1 cut(s)	1838
Bpu14I	TTCGAA	1 cut(s)	273
BpuEI	CTTGAG	2 cut(s)	1206, 1982
BsaBI	GATNNNNATC	3 cut(s)	831, 1246, 2025
BsaJI	CCNNGG	8 cut(s)	1584, 1610, 1747, 2809, 2821, 3034, 3062, 3162
BsaWI	WCCGGW	2 cut(s)	376, 1989
BsaXI	ACNNNNNCTCC	6 cut(s)	880, 910, 1497, 1527, 2893, 2923
Bsc4I	CCNNNNNNNGG	3 cut(s)	236, 1024, 1266
Bse1I	ACTGG	9 cut(s)	101, 151, 160, 178, 1267, 1710, 2293, 2843, 3242
Bse3DI	GCAATG	1 cut(s)	618
Bse8I	GATNNNNATC	3 cut(s)	831, 1246, 2025
BseAI	TCCGGA	2 cut(s)	376, 1989
BseBI	CCWGG	5 cut(s)	870, 1503, 1586, 2810, 3064
BseDI	CCNNGG	8 cut(s)	1584, 1610, 1747, 2809, 2821, 3034, 3062, 3162
BseJI	GATNNNNATC	3 cut(s)	831, 1246, 2025
BseLI	CCNNNNNNNGG	3 cut(s)	236, 1024, 1266
BseMI	GCAATG	1 cut(s)	618
BseNI	ACTGG	9 cut(s)	101, 151, 160, 178, 1267, 1710, 2293, 2843, 3242
BseRI	GAGGAG	2 cut(s)	100, 901
BseSI	GKGCMC	3 cut(s)	1312, 2092, 2507
BseXI	GCAGC	7 cut(s)	1057, 1089, 1183, 1186, 1633, 2060, 2627
BseYI	CCCAGC	1 cut(s)	2630
BsgI	GTGCAG	2 cut(s)	818, 2634
BshFI	GGCC	5 cut(s)	644, 1691, 1884, 2187, 2820
BshNI	GGYRCC	1 cut(s)	850
BsiHKAI	GWGCWC	4 cut(s)	92, 726, 2477, 2507
BsiHKCI	CYCGRG	1 cut(s)	3002
BsiSI	CCGG	3 cut(s)	377, 1990, 2751
BslFI	GGGAC	3 cut(s)	382, 589, 2955
BslI	CCNNNNNNNGG	3 cut(s)	236, 1024, 1266
BsmAI	GTCTC	5 cut(s)	300, 905, 2000, 2682, 2896
BsmFI	GGGAC	3 cut(s)	382, 589, 2955
BsnI	GGCC	5 cut(s)	644, 1691, 1884, 2187, 2820
BsoBI	CYCGRG	1 cut(s)	3002
Bsp119I	TTCGAA	1 cut(s)	273
Bsp1286I	GDGCHC	6 cut(s)	92, 726, 1312, 2092, 2477, 2507
Bsp13I	TCCGGA	2 cut(s)	376, 1989
Bsp1720I	GCTNAGC	1 cut(s)	1838
Bsp19I	CCATGG	1 cut(s)	2821
BspACI	CCGC	5 cut(s)	280, 573, 1367, 1411, 2957
BspANI	GGCC	5 cut(s)	644, 1691, 1884, 2187, 2820
BspEI	TCCGGA	2 cut(s)	376, 1989
BspHI	TCATGA	2 cut(s)	2680, 3118
BspLI	GGNNCC	6 cut(s)	203, 258, 811, 852, 1359, 1608
BspMAI	CTGCAG	3 cut(s)	1107, 1623, 2342
BspPI	GGATC	9 cut(s)	91, 247, 693, 774, 827, 1046, 1815, 2463, 3098
BspQI	GCTCTTC	1 cut(s)	2482
BspT104I	TTCGAA	1 cut(s)	273
BspT107I	GGYRCC	1 cut(s)	850
BsrBI	CCGCTC	1 cut(s)	573
BsrDI	GCAATG	1 cut(s)	618
BsrI	ACTGG	9 cut(s)	101, 151, 160, 178, 1267, 1710, 2293, 2843, 3242
BssECI	CCNNGG	8 cut(s)	1584, 1610, 1747, 2809, 2821, 3034, 3062, 3162
BssT1I	CCWWGG	3 cut(s)	1610, 2821, 3034
Bst2UI	CCWGG	5 cut(s)	870, 1503, 1586, 2810, 3064
Bst6I	CTCTTC	2 cut(s)	1439, 2482
BstAPI	GCANNNNNTGC	1 cut(s)	2337
BstBI	TTCGAA	1 cut(s)	273
BstC8I	GCNNGC	4 cut(s)	508, 546, 1312, 2530
BstDSI	CCRYGG	2 cut(s)	1747, 2821
BstH2I	RGCGCY	2 cut(s)	1627, 1667
BstHHI	GCGC	4 cut(s)	1287, 1626, 1666, 2437
BstMAI	GTCTC	5 cut(s)	300, 905, 2000, 2682, 2896
BstNI	CCWGG	5 cut(s)	870, 1503, 1586, 2810, 3064
BstNSI	RCATGY	3 cut(s)	1151, 2511, 2528
BstPAI	GACNNNNGTC	1 cut(s)	1892
BstSCI	CCNGG	5 cut(s)	868, 1501, 1584, 2808, 3062
BstSFI	CTRYAG	5 cut(s)	1103, 1619, 1685, 2338, 2665
BstSLI	GKGCMC	3 cut(s)	1312, 2092, 2507
BstV1I	GCAGC	7 cut(s)	1057, 1089, 1183, 1186, 1633, 2060, 2627
BstV2I	GAAGAC	3 cut(s)	1746, 1914, 2066
BstX2I	RGATCY	6 cut(s)	96, 685, 1051, 1807, 2455, 2641
BstXI	CCANNNNNNTGG	2 cut(s)	392, 2838
BstYI	RGATCY	6 cut(s)	96, 685, 1051, 1807, 2455, 2641
BsuI	GTATCC	1 cut(s)	876
BsuRI	GGCC	5 cut(s)	644, 1691, 1884, 2187, 2820
BtgI	CCRYGG	2 cut(s)	1747, 2821
BtgZI	GCGATG	1 cut(s)	837
BtsI	GCAGTG	3 cut(s)	806, 1564, 2347
Cac8I	GCNNGC	4 cut(s)	508, 546, 1312, 2530
CaiI	CAGNNNCTG	3 cut(s)	226, 2129, 2500
CciI	TCATGA	2 cut(s)	2680, 3118
CfoI	GCGC	4 cut(s)	1287, 1626, 1666, 2437
Cfr13I	GGNCC	3 cut(s)	642, 2186, 2698
Csp6I	GTAC	1 cut(s)	1119
CspCI	CAANNNNNGTGG	2 cut(s)	825, 860
CviQI	GTAC	1 cut(s)	1119
DraI	TTTAAA	1 cut(s)	1048
DraIII	CACNNNGTG	1 cut(s)	1266
DrdI	GACNNNNNNGTC	1 cut(s)	1168
DseDI	GACNNNNNNGTC	1 cut(s)	1168
Eam1104I	CTCTTC	2 cut(s)	1439, 2482
EarI	CTCTTC	2 cut(s)	1439, 2482
Ecl136II	GAGCTC	2 cut(s)	724, 2475
Eco130I	CCWWGG	3 cut(s)	1610, 2821, 3034
Eco147I	AGGCCT	1 cut(s)	1691
Eco24I	GRGCYC	2 cut(s)	726, 2477
Eco47I	GGWCC	1 cut(s)	2698
Eco47III	AGCGCT	2 cut(s)	1625, 1665
Eco53kI	GAGCTC	2 cut(s)	724, 2475
Eco57I	CTGAAG	7 cut(s)	803, 1420, 1884, 2220, 2307, 2463, 2994
Eco88I	CYCGRG	1 cut(s)	3002
EcoICRI	GAGCTC	2 cut(s)	724, 2475
EcoRI	GAATTC	1 cut(s)	416
EcoRII	CCWGG	5 cut(s)	868, 1501, 1584, 2808, 3062
EcoT14I	CCWWGG	3 cut(s)	1610, 2821, 3034
EcoT22I	ATGCAT	2 cut(s)	620, 1674
EcoT38I	GRGCYC	2 cut(s)	726, 2477
ErhI	CCWWGG	3 cut(s)	1610, 2821, 3034
FaqI	GGGAC	3 cut(s)	382, 589, 2955
FauI	CCCGC	2 cut(s)	566, 1404
FbaI	TGATCA	3 cut(s)	697, 2212, 2515
Fnu4HI	GCNGC	7 cut(s)	1071, 1103, 1197, 1200, 1622, 2074, 2616
FriOI	GRGCYC	2 cut(s)	726, 2477
Fsp4HI	GCNGC	7 cut(s)	1071, 1103, 1197, 1200, 1622, 2074, 2616
GlaI	GCGC	4 cut(s)	1286, 1625, 1665, 2436
GluI	GCNGC	7 cut(s)	1071, 1103, 1197, 1200, 1622, 2074, 2616
GsaI	CCCAGC	1 cut(s)	2634
GsuI	CTGGAG	2 cut(s)	1524, 2781
HaeII	RGCGCY	2 cut(s)	1627, 1667
HaeIII	GGCC	5 cut(s)	644, 1691, 1884, 2187, 2820
HapII	CCGG	3 cut(s)	377, 1990, 2751
HhaI	GCGC	4 cut(s)	1287, 1626, 1666, 2437
Hin6I	GCGC	4 cut(s)	1285, 1624, 1664, 2435
HinP1I	GCGC	4 cut(s)	1285, 1624, 1664, 2435
HindIII	AAGCTT	2 cut(s)	737, 1442
HpaII	CCGG	3 cut(s)	377, 1990, 2751
Hpy166II	GTNNAC	4 cut(s)	718, 2505, 2725, 2785
Hpy8I	GTNNAC	4 cut(s)	718, 2505, 2725, 2785
HpyCH4IV	ACGT	3 cut(s)	761, 1169, 2658
HpySE526I	ACGT	3 cut(s)	761, 1169, 2658
HspAI	GCGC	4 cut(s)	1285, 1624, 1664, 2435
Kpn2I	TCCGGA	2 cut(s)	376, 1989
Ksp22I	TGATCA	3 cut(s)	697, 2212, 2515
LguI	GCTCTTC	1 cut(s)	2482
LmnI	GCTCC	4 cut(s)	87, 207, 422, 2800
Lsp1109I	GCAGC	7 cut(s)	1057, 1089, 1183, 1186, 1633, 2060, 2627
LweI	GCATC	7 cut(s)	169, 307, 360, 484, 1051, 1951, 3067
MaeII	ACGT	3 cut(s)	761, 1169, 2658
MaeIII	GTNAC	6 cut(s)	1234, 1535, 1705, 2140, 2997, 3155
MbiI	CCGCTC	1 cut(s)	573
MfeI	CAATTG	3 cut(s)	693, 915, 1074
MflI	RGATCY	6 cut(s)	96, 685, 1051, 1807, 2455, 2641
MhlI	GDGCHC	6 cut(s)	92, 726, 1312, 2092, 2477, 2507
MlyI	GAGTC	1 cut(s)	2002
MmeI	TCCRAC	3 cut(s)	744, 1897, 2676
Mph1103I	ATGCAT	2 cut(s)	620, 1674
MroI	TCCGGA	2 cut(s)	376, 1989
MseI	TTAA	9 cut(s)	170, 735, 1047, 1757, 2156, 2363, 2444, 2541, 2690
MslI	CAYNNNNRTG	2 cut(s)	1152, 2652
MspA1I	CMGCKG	3 cut(s)	1411, 1634, 1837
MspI	CCGG	3 cut(s)	377, 1990, 2751
MspR9I	CCNGG	5 cut(s)	870, 1503, 1586, 2810, 3064
MunI	CAATTG	3 cut(s)	693, 915, 1074
MvaI	CCWGG	5 cut(s)	870, 1503, 1586, 2810, 3064
NcoI	CCATGG	1 cut(s)	2821
NlaIV	GGNNCC	6 cut(s)	203, 258, 811, 852, 1359, 1608
NmuCI	GTSAC	4 cut(s)	1535, 1705, 2140, 3155
NsiI	ATGCAT	2 cut(s)	620, 1674
NspI	RCATGY	3 cut(s)	1151, 2511, 2528
NspV	TTCGAA	1 cut(s)	273
PaeR7I	CTCGAG	1 cut(s)	3002
PagI	TCATGA	2 cut(s)	2680, 3118
PceI	AGGCCT	1 cut(s)	1691
PciI	ACATGT	1 cut(s)	2507
PciSI	GCTCTTC	1 cut(s)	2482
PflMI	CCANNNNNTGG	2 cut(s)	1024, 1266
PfoI	TCCNGGA	1 cut(s)	868
PkrI	GCNGC	7 cut(s)	1072, 1104, 1198, 1201, 1623, 2075, 2617
PleI	GAGTC	1 cut(s)	2001
PpsI	GAGTC	1 cut(s)	2001
PscI	ACATGT	1 cut(s)	2507
PshAI	GACNNNNGTC	1 cut(s)	1892
Psp124BI	GAGCTC	2 cut(s)	726, 2477
Psp6I	CCWGG	5 cut(s)	868, 1501, 1584, 2808, 3062
PspFI	CCCAGC	1 cut(s)	2630
PspGI	CCWGG	5 cut(s)	868, 1501, 1584, 2808, 3062
PspN4I	GGNNCC	6 cut(s)	203, 258, 811, 852, 1359, 1608
PspPI	GGNCC	3 cut(s)	642, 2186, 2698
PspXI	VCTCGAGB	1 cut(s)	3002
PsrI	GAACNNNNNNTAC	2 cut(s)	865, 897
PstI	CTGCAG	3 cut(s)	1107, 1623, 2342
PstNI	CAGNNNCTG	3 cut(s)	226, 2129, 2500
PsuI	RGATCY	6 cut(s)	96, 685, 1051, 1807, 2455, 2641
PvuII	CAGCTG	2 cut(s)	1634, 1837
RsaI	GTAC	1 cut(s)	1120
RsaNI	GTAC	1 cut(s)	1119
RseI	CAYNNNNRTG	2 cut(s)	1152, 2652
SacI	GAGCTC	2 cut(s)	726, 2477
SapI	GCTCTTC	1 cut(s)	2482
SaqAI	TTAA	9 cut(s)	170, 735, 1047, 1757, 2156, 2363, 2444, 2541, 2690
SatI	GCNGC	7 cut(s)	1071, 1103, 1197, 1200, 1622, 2074, 2616
Sau96I	GGNCC	3 cut(s)	642, 2186, 2698
SchI	GAGTC	1 cut(s)	2002
ScrFI	CCNGG	5 cut(s)	870, 1503, 1586, 2810, 3064
SduI	GDGCHC	6 cut(s)	92, 726, 1312, 2092, 2477, 2507
SfaNI	GCATC	7 cut(s)	169, 307, 360, 484, 1051, 1951, 3067
SfcI	CTRYAG	5 cut(s)	1103, 1619, 1685, 2338, 2665
Sfr274I	CTCGAG	1 cut(s)	3002
SfuI	TTCGAA	1 cut(s)	273
SinI	GGWCC	1 cut(s)	2698
SlaI	CTCGAG	1 cut(s)	3002
SmiMI	CAYNNNNRTG	2 cut(s)	1152, 2652
SmlI	CTYRAG	3 cut(s)	1221, 1997, 3002
SmoI	CTYRAG	3 cut(s)	1221, 1997, 3002
SseBI	AGGCCT	1 cut(s)	1691
SsiI	CCGC	5 cut(s)	280, 573, 1367, 1411, 2957
SspI	AATATT	1 cut(s)	2266
SstI	GAGCTC	2 cut(s)	726, 2477
StuI	AGGCCT	1 cut(s)	1691
StyD4I	CCNGG	5 cut(s)	868, 1501, 1584, 2808, 3062
StyI	CCWWGG	3 cut(s)	1610, 2821, 3034
TaiI	ACGT	3 cut(s)	764, 1172, 2661
Tru1I	TTAA	9 cut(s)	170, 735, 1047, 1757, 2156, 2363, 2444, 2541, 2690
Tru9I	TTAA	9 cut(s)	170, 735, 1047, 1757, 2156, 2363, 2444, 2541, 2690
TseFI	GTSAC	4 cut(s)	1535, 1705, 2140, 3155
TseI	GCWGC	7 cut(s)	1070, 1102, 1196, 1199, 1621, 2073, 2615
Tsp45I	GTSAC	4 cut(s)	1535, 1705, 2140, 3155
TspDTI	ATGAA	6 cut(s)	204, 1125, 2607, 2857, 3027, 3057
TspGWI	ACGGA	7 cut(s)	194, 873, 1166, 1684, 1736, 2078, 2550
Van91I	CCANNNNNTGG	2 cut(s)	1024, 1266
VneI	GTGCAC	1 cut(s)	2503
VpaK11BI	GGWCC	1 cut(s)	2698
XapI	RAATTY	9 cut(s)	416, 463, 625, 1043, 1350, 1565, 1752, 2167, 2606
XbaI	TCTAGA	2 cut(s)	1336, 2305
XceI	RCATGY	3 cut(s)	1151, 2511, 2528
XcmI	CCANNNNNNNNNTGG	3 cut(s)	843, 2838, 2895
XhoI	CTCGAG	1 cut(s)	3002
Zsp2I	ATGCAT	2 cut(s)	620, 1674

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.