Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0006775

Physical Location & Seq

Genomic Coordinates Forward (+)

15204129 .. 15211187

Gene Structure 7059 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1192800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3240 bp

ATGGGTTGTGTTTATTCAAGAGTTTGCATTGGAGAGCTTTGTATTCCAAGAGTTCCGAAATTGAAAGAGAGTCAGGATGTGAGGAGCACTGAGATCCCAGTGTTCTCCCCTACTTCCTCCAATGGAGAAGTAGGTGAGCTCAGAGACCAATTCAACCAGTCTGGCTTAACTGGGGATGCTGAGGTGGGTATAACTAGGCTCCGTAGGGTTTCATCACAGTTTCTGCCCCCCAATGGGTCAAGAACTGTCAAGGTCCCTTCAGGAAACTTTGAAATGCGGTATTCGTATTTGTCTCAGAGAGGTTATTACCCTGATGCTCTTGATAAGGCTAACCAAGATAGCTTTTGCATCCACACGCCATTTGGGACAAACCCGGATGATCATTTCTTTGGGGTCTTTGATGGTCATGGAGAATTCGGAGCTCAGTGTTCGCAGTTTGTGAAAAGAAAGTTGTGTGAGAATTTGCTTAGGAATAATAAGTTTCAAGTGGATGCTGTCGAGGCATGCCATTCTGCATTTATCGCAACCAATTCTCAGTTGCACGCTGATATTTTGGATGATAGCATGAGCGGGACAACTGCAATTACGGTTTTGGTTCGAGGTAGGACGATATGCATTGCGAATTCGGGTGATTCAAGGGCTGTTATAGCAGAGAGGAAAGGGGATGACATTGTAGCTGTGGACCTTTCAATTGATCAAACCCCATTTAGAGTGGACGAGCTCGAAAGGGTTAAGCTTTGTGGTGCAAGAGTTCTTACATTGGATCAAATTGAAGGGCTGAAGAACCCAGATGTGCAGTGTTGGGGAACCGAAGAAAGCGATGATGGTGATCCACCTAGATTGTGGGTGCCAAATGGAATGTATCCTGGGACAGCTTTTACAAGGAGTCTTGGTGATTCAATTGCTGAGACAATTGGGGTTGTTGCGAACCCTGAAATTGTGGTTTTAGAGCTTACACAGAATAATCCTTTCTTTGTTCTTGCTAGTGATGGAGTTTTTGAGTTCCTTTCTAGCCAAGCTGTGGTGGATATGGTTGCAAAATTTAAAGATCCCCGTGATGCTTGTGCTGCAATTGTAGCTGAATCGTATAAACTCTGGCTGCAGTATGAAACTCGTACAGATGATATTACAGTGATCGTTGTGCATGTAAATGGGCTGACTGATATGTCCGTTGGTCAATCAATAAGTTCTGCTACTGCTTTAAGACCACCTGTTCCTCAAGTTGTAGAGGTTACAGGATGTGAATCTCCTTCACCGTCTGGGTGGAACTCTAGGAACCAGCGCACCAGACATGATTTGTCAAAGGCACGCCTTCGTGCGATTGAGAGTTCTCTAGAAAATGGGCAAATTTGGGTTCCTCCATCTCCATCCCACAGAAAGACTTGGGAGGAAGAAGCACACATTGAGCGGGCTTTGCATGACCATTTCCTCTTCAGAAAGCTTACTGATTCTCAGTGTCAGGTGTTATTGGACTGCATGGAAAGGGTTGAGTTCCAGCCTGGAGATGTTGTTGTTAGACAGGGTGGTGAAGGTGACTGCTTCTATGTTGTTGGCAGTGGAGAATTTGAGGTGTTGGCAACCCAGGAAGAAAAGAATGGAGAGGTTCCGAGGGTTCTGCAGCACTATACAGCTGATAAACTGTCATCCTTTGGAGAACTAGCGCTAATGCATAACAAACCACTCCAGGCCTCTGTTCGTGCTGTTACCAGTGGAACTCTTTGGGCTTTGAAAAGAGAAGACTTCCGTGGAATTTTAACGTCAGAGTTTTCTAATTTGTCATATTTGAAGTTGCTTCGATCAGTGGATCTTCTATCAAGGCTGACAATCTTACAGCTGAGCCATATTGCCGATTCTCTGTCTGAAGTTTCCTTCTCACAAGGGCAGACGATAGTCAGCGAGAGTGAACGCCTAGTTGGATTATACATTATCCAGAAGGGAAAAGTGAGGATTACTTTTGATGCAAATTCAGTTAGTAGTCCAGTTGTCCGGAGTCTGAAGTCTGACTATCAAAAAGAAGATGATCATCCTCAGAGCAGTAAAGAGCTCTCGGTGGAGAAGACGGAGGGAAGCTGCTTTGGTGAATGGGCACTTCTTGGAGAACATATTGATTTGTTTACTGCAGTTGCTGTGGGAGATGTCACCTGTGCTGTTTTAACAAAGGAAAATTTTGATTCAGTCGTTGGCCCTTTGACAAAGCTTTCTCAGGATGATCGGAAGTCAAGGGATTATTCTTCTGATGTTGCAAAGGGATCTGCCAAGAACGTTGATATTTCTGCTCTTTCTAAAGTCCAGTTTTCTGATCTGGATTGGAGAACAATTTTATATAGCACTGACTGCAGTGAGATTGGACTTGCATGTTTAAGAGATTCAGAAAAATTGCTTAGTTTGAAAAGGTTTTCAAAGCAAAAGGTCAGAAGGATGGGAAAGGAAGCACAAGTGTTGAGAGAGAAGGATCTAATCAAGAGTATGAGTTCTTCAGCGTGTGTGCCACAGTTTCTGTGTACATGTGTTGATCAGACACATGCTGGCATACTGTATAATACTTGCCTTGCTTGTCCGTTGGCTTCGATACTTCGCACTCCCCTTGATGAACCATCTGCCAAATTTTGTGCAGCCTCTCTAGTTGCTGGTTTGGCAGATCTCCATAAGAATGACGTTCTCTACAGAGGCTTGTCTCCTGATGTTTTAATGTTGGACCAAACAGGATATTTGCAGCTAGTAGACTTCAGGTTTGGCAAGAAGTTATCCGGTGAAAGGACATATACAATTTGTGGAATGGTGGACTTTTTAGCTCCAGAGGTAGTCCAGGGAAAAGGCCATGGTTTCCCAGCTGACTGGTGGGCATTAGGAGTGTTGATATACTTCATGCTACAGGGTGAAATGCCATTCGGCTCATGGAGACAAAGCGAGCTTGATACATTTGCAAAGATCGCAAAGGGACAGCTAACTCTACCGCAAACTTTTAGTCCTGAAGTTGTTGATCTCATAACTAAGTTACTCGAGGTTGATGAAAACACCAGACTAGGAAGCCAAGGTTATGAATCTGTCAAAAGGCATCCCTGGTTTGATGGCATGGATTGGGAAGGGATCAAAGACAGTAGTTTGCCTGTTCCTCATGAGATCACATCTCGTATAACTCAACATTTGGGAAGTCACTCCGAGGACTGTTCTGTTCCTCTAGGTTCTCCATCTCGAAATGGAGAAGAACTCGATACTCCTGAATGGTTTGACGACTGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko04022 ko04540 ko04611 ko04713 ko04714 ko04730 ko04740 ko04923 ko04924 ko04970 map04022 map04540 map04611 map04713 map04714 map04730 map04740 map04923 map04924 map04970

Pfam Domains

Protein Families

PP2C Pkinase cNMP_binding

Protein Analysis

1080

Amino Acids

119.5

Weight (kDa)

5.14

Isoelectric Point (pI)

43.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PP2C	PF00481	119 - 361	2.1e-31	Protein phosphatase 2C
cNMP_binding	PF00027	496 - 586	2.8e-16	Cyclic nucleotide-binding domain
cNMP_binding	PF00027	622 - 726	9.7e-09	Cyclic nucleotide-binding domain
Pkinase	PF00069	784 - 1022	1e-37	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	809 - 1007	5.1e-12	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0006775)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G20050 AT2G20050
fragaria_vesca	FvH4_1g05740 FvH4_1g05740 FvH4_1g05740
malus_domestica	MD02G1058500.v1.1 MD15G1192800.v1.1
prunus_persica	Prupe.7G224500_v2.0.a1 Prupe.7G224500_v2.0.a1 Prupe.7G224500_v2.0.a1 Prupe.7G224500_v2.0.a1
pyrus_communis	pycom02g04740 pycom15g17250 pycom15g17260
rosa_chinensis	RchiOBHm_Chr2g0091381
rosa_laevigata	RLG00000016219
rosa_multiflora	Rmu_ssc0000333.1_g000004 Rmu_ssc0000333.1_g000011
rosa_roxburghii	Rroxscaffold_2G00150130
rosa_rugosa	Rorug02G0017200 Rorug02G0017300
rosa_samantha	Rh2AG062300 Rh2BG061300 Rh2CG063400 Rh2DG061900
rosa_wichuraiana	Rw2G005790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1165
AccB1I	GGYRCC	1 cut(s)	847
AccB7I	CCANNNNNTGG	1 cut(s)	1021
AccBSI	CCGCTC	2 cut(s)	570, 1408
AccI	GTMKAC	1 cut(s)	2721
AccIII	TCCGGA	1 cut(s)	1986
AciI	CCGC	4 cut(s)	277, 570, 1408, 2954
AclI	AACGTT	1 cut(s)	2262
AclWI	GGATC	8 cut(s)	88, 771, 824, 1043, 1812, 2257, 2460, 3095
AcuI	CTGAAG	8 cut(s)	243, 800, 1417, 1881, 2015, 2460, 2710, 2991
AfaI	GTAC	2 cut(s)	1117, 2503
AfeI	AGCGCT	1 cut(s)	1662
AfiI	CCNNNNNNNGG	4 cut(s)	233, 234, 1021, 2754
AflIII	ACRYGT	1 cut(s)	2504
AjnI	CCWGG	6 cut(s)	865, 1498, 1581, 1683, 2805, 3059
AjuI	GAANNNNNNNTTGG	2 cut(s)	1896, 1928
Alw21I	GWGCWC	5 cut(s)	89, 141, 424, 723, 2046
Alw26I	GTCTC	5 cut(s)	138, 297, 902, 2679, 2893
AlwI	GGATC	8 cut(s)	88, 771, 824, 1043, 1812, 2257, 2460, 3095
AlwNI	CAGNNNCTG	3 cut(s)	223, 2126, 2497
Ama87I	CYCGRG	1 cut(s)	2999
Aor13HI	TCCGGA	1 cut(s)	1986
Aor51HI	AGCGCT	1 cut(s)	1662
AoxI	GGCC	3 cut(s)	1686, 2182, 2815
ApeKI	GCWGC	6 cut(s)	1067, 1099, 1618, 2070, 2612, 2713
ArsI	GACNNNNNNTTYG	4 cut(s)	2714, 2746, 2950, 2982
AspLEI	GCGC	2 cut(s)	1284, 1663
AspS9I	GGNCC	4 cut(s)	253, 682, 2183, 2695
AsuC2I	CCSGG	1 cut(s)	374
AvaI	CYCGRG	1 cut(s)	2999
AvaII	GGWCC	3 cut(s)	253, 682, 2695
BaeGI	GKGCMC	1 cut(s)	2089
BanI	GGYRCC	1 cut(s)	847
BanII	GRGCYC	4 cut(s)	141, 424, 723, 2046
BarI	GAAGNNNNNNTAC	2 cut(s)	2458, 2490
BbsI	GAAGAC	2 cut(s)	1743, 2063
Bbv12I	GWGCWC	5 cut(s)	89, 141, 424, 723, 2046
BbvCI	CCTCAGC	1 cut(s)	180
BbvI	GCAGC	6 cut(s)	1054, 1086, 1630, 2057, 2624, 2725
BccI	CCATC	9 cut(s)	395, 818, 983, 1369, 1375, 2413, 2602, 3062, 3196
BcgI	CGANNNNNNTGC	2 cut(s)	1597, 1631
BciT130I	CCWGG	6 cut(s)	867, 1500, 1583, 1685, 2807, 3061
BciVI	GTATCC	1 cut(s)	873
BclI	TGATCA	4 cut(s)	379, 694, 2020, 2512
BcnI	CCSGG	1 cut(s)	374
BcoDI	GTCTC	5 cut(s)	138, 297, 902, 2679, 2893
BfmI	CTRYAG	6 cut(s)	1100, 1616, 2118, 2335, 2662, 2870
BfoI	RGCGCY	1 cut(s)	1664
BfuI	GTATCC	1 cut(s)	873
BglII	AGATCT	1 cut(s)	2638
BisI	GCNGC	6 cut(s)	1068, 1100, 1619, 2071, 2613, 2714
BlpI	GCTNAGC	1 cut(s)	1835
BlsI	GCNGC	6 cut(s)	1069, 1101, 1620, 2072, 2614, 2715
Bme1390I	CCNGG	7 cut(s)	374, 867, 1500, 1583, 1685, 2807, 3061
Bme18I	GGWCC	3 cut(s)	253, 682, 2695
BmeT110I	CYCGRG	1 cut(s)	2999
BmgT120I	GGNCC	4 cut(s)	253, 682, 2183, 2695
BmiI	GGNNCC	7 cut(s)	200, 255, 808, 849, 1277, 1356, 1605
BmrFI	CCNGG	7 cut(s)	374, 867, 1500, 1583, 1685, 2807, 3061
BmrI	ACTGGG	2 cut(s)	92, 180
BmsI	GCATC	7 cut(s)	166, 304, 357, 481, 1048, 1948, 3064
BmuI	ACTGGG	2 cut(s)	92, 180
BoxI	GACNNNNGTC	1 cut(s)	1889
BpiI	GAAGAC	2 cut(s)	1743, 2063
BplI	GAGNNNNNCTC	2 cut(s)	3192, 3224
BpmI	CTGGAG	3 cut(s)	1521, 1667, 2778
Bpu10I	CCTNAGC	2 cut(s)	180, 467
Bpu1102I	GCTNAGC	1 cut(s)	1835
BpuEI	CTTGAG	1 cut(s)	1203
BpuMI	CCSGG	1 cut(s)	374
BsaBI	GATNNNNATC	3 cut(s)	828, 1243, 2022
BsaI	GGTCTC	1 cut(s)	138
BsaJI	CCNNGG	9 cut(s)	866, 1581, 1607, 1744, 2806, 2818, 3031, 3059, 3159
BsaWI	WCCGGW	2 cut(s)	1986, 2747
BsaXI	ACNNNNNCTCC	4 cut(s)	877, 907, 1494, 1524
Bsc4I	CCNNNNNNNGG	4 cut(s)	233, 234, 1021, 2754
Bse1I	ACTGG	8 cut(s)	98, 157, 175, 1707, 1979, 2290, 2840, 3239
Bse3DI	GCAATG	1 cut(s)	615
Bse8I	GATNNNNATC	3 cut(s)	828, 1243, 2022
BseAI	TCCGGA	1 cut(s)	1986
BseBI	CCWGG	6 cut(s)	867, 1500, 1583, 1685, 2807, 3061
BseDI	CCNNGG	9 cut(s)	866, 1581, 1607, 1744, 2806, 2818, 3031, 3059, 3159
BseJI	GATNNNNATC	3 cut(s)	828, 1243, 2022
BseLI	CCNNNNNNNGG	4 cut(s)	233, 234, 1021, 2754
BseMI	GCAATG	1 cut(s)	615
BseNI	ACTGG	8 cut(s)	98, 157, 175, 1707, 1979, 2290, 2840, 3239
BseRI	GAGGAG	1 cut(s)	97
BseSI	GKGCMC	1 cut(s)	2089
BseXI	GCAGC	6 cut(s)	1054, 1086, 1630, 2057, 2624, 2725
BseYI	CCCAGC	1 cut(s)	2827
BsgI	GTGCAG	2 cut(s)	815, 2631
BshFI	GGCC	3 cut(s)	1688, 2184, 2817
BshNI	GGYRCC	1 cut(s)	847
BsiHKAI	GWGCWC	5 cut(s)	89, 141, 424, 723, 2046
BsiHKCI	CYCGRG	1 cut(s)	2999
BsiSI	CCGG	3 cut(s)	374, 1987, 2748
BslFI	GGGAC	5 cut(s)	239, 379, 586, 883, 2952
BslI	CCNNNNNNNGG	4 cut(s)	233, 234, 1021, 2754
BsmAI	GTCTC	5 cut(s)	138, 297, 902, 2679, 2893
BsmFI	GGGAC	5 cut(s)	239, 379, 586, 883, 2952
BsnI	GGCC	3 cut(s)	1688, 2184, 2817
Bso31I	GGTCTC	1 cut(s)	138
BsoBI	CYCGRG	1 cut(s)	2999
Bsp1286I	GDGCHC	6 cut(s)	89, 141, 424, 723, 2046, 2089
Bsp13I	TCCGGA	1 cut(s)	1986
Bsp1407I	TGTACA	1 cut(s)	2501
Bsp1720I	GCTNAGC	1 cut(s)	1835
Bsp19I	CCATGG	1 cut(s)	2818
BspACI	CCGC	4 cut(s)	277, 570, 1408, 2954
BspANI	GGCC	3 cut(s)	1688, 2184, 2817
BspEI	TCCGGA	1 cut(s)	1986
BspHI	TCATGA	1 cut(s)	3115
BspLI	GGNNCC	7 cut(s)	200, 255, 808, 849, 1277, 1356, 1605
BspMAI	CTGCAG	4 cut(s)	1104, 1620, 2122, 2339
BspPI	GGATC	8 cut(s)	88, 771, 824, 1043, 1812, 2257, 2460, 3095
BspT107I	GGYRCC	1 cut(s)	847
BspTNI	GGTCTC	1 cut(s)	138
BsrBI	CCGCTC	2 cut(s)	570, 1408
BsrDI	GCAATG	1 cut(s)	615
BsrGI	TGTACA	1 cut(s)	2501
BsrI	ACTGG	8 cut(s)	98, 157, 175, 1707, 1979, 2290, 2840, 3239
BssECI	CCNNGG	9 cut(s)	866, 1581, 1607, 1744, 2806, 2818, 3031, 3059, 3159
BssT1I	CCWWGG	2 cut(s)	2818, 3031
Bst2UI	CCWGG	6 cut(s)	867, 1500, 1583, 1685, 2807, 3061
Bst6I	CTCTTC	1 cut(s)	1436
BstAPI	GCANNNNNTGC	1 cut(s)	2334
BstAUI	TGTACA	1 cut(s)	2501
BstC8I	GCNNGC	6 cut(s)	505, 543, 1309, 1410, 2527, 2909
BstDSI	CCRYGG	2 cut(s)	1744, 2818
BstH2I	RGCGCY	1 cut(s)	1664
BstHHI	GCGC	2 cut(s)	1284, 1663
BstMAI	GTCTC	5 cut(s)	138, 297, 902, 2679, 2893
BstMWI	GCNNNNNNNGC	9 cut(s)	500, 521, 647, 1067, 1076, 1414, 2334, 2633, 2930
BstNI	CCWGG	6 cut(s)	867, 1500, 1583, 1685, 2807, 3061
BstNSI	RCATGY	5 cut(s)	507, 1148, 2358, 2508, 2525
BstPAI	GACNNNNGTC	1 cut(s)	1889
BstSCI	CCNGG	7 cut(s)	372, 865, 1498, 1581, 1683, 2805, 3059
BstSFI	CTRYAG	6 cut(s)	1100, 1616, 2118, 2335, 2662, 2870
BstSLI	GKGCMC	1 cut(s)	2089
BstV1I	GCAGC	6 cut(s)	1054, 1086, 1630, 2057, 2624, 2725
BstV2I	GAAGAC	2 cut(s)	1743, 2063
BstX2I	RGATCY	6 cut(s)	93, 1048, 1804, 2249, 2452, 2638
BstXI	CCANNNNNNTGG	1 cut(s)	2835
BstYI	RGATCY	6 cut(s)	93, 1048, 1804, 2249, 2452, 2638
BsuI	GTATCC	1 cut(s)	873
BsuRI	GGCC	3 cut(s)	1688, 2184, 2817
BtgI	CCRYGG	2 cut(s)	1744, 2818
BtgZI	GCGATG	1 cut(s)	834
BtsI	GCAGTG	3 cut(s)	803, 1561, 2344
Cac8I	GCNNGC	6 cut(s)	505, 543, 1309, 1410, 2527, 2909
CaiI	CAGNNNCTG	3 cut(s)	223, 2126, 2497
CciI	TCATGA	1 cut(s)	3115
CfoI	GCGC	2 cut(s)	1284, 1663
Cfr13I	GGNCC	4 cut(s)	253, 682, 2183, 2695
Csp6I	GTAC	2 cut(s)	1116, 2502
CspCI	CAANNNNNGTGG	2 cut(s)	822, 857
CviQI	GTAC	2 cut(s)	1116, 2502
DraI	TTTAAA	1 cut(s)	1045
DrdI	GACNNNNNNGTC	1 cut(s)	1165
DseDI	GACNNNNNNGTC	1 cut(s)	1165
Eam1104I	CTCTTC	1 cut(s)	1436
EarI	CTCTTC	1 cut(s)	1436
Ecl136II	GAGCTC	4 cut(s)	139, 422, 721, 2044
Eco130I	CCWWGG	2 cut(s)	2818, 3031
Eco147I	AGGCCT	1 cut(s)	1688
Eco24I	GRGCYC	4 cut(s)	141, 424, 723, 2046
Eco31I	GGTCTC	1 cut(s)	138
Eco47I	GGWCC	3 cut(s)	253, 682, 2695
Eco47III	AGCGCT	1 cut(s)	1662
Eco53kI	GAGCTC	4 cut(s)	139, 422, 721, 2044
Eco57I	CTGAAG	8 cut(s)	243, 800, 1417, 1881, 2015, 2460, 2710, 2991
Eco88I	CYCGRG	1 cut(s)	2999
EcoICRI	GAGCTC	4 cut(s)	139, 422, 721, 2044
EcoO109I	RGGNCCY	1 cut(s)	253
EcoRI	GAATTC	2 cut(s)	413, 622
EcoRII	CCWGG	6 cut(s)	865, 1498, 1581, 1683, 2805, 3059
EcoT14I	CCWWGG	2 cut(s)	2818, 3031
EcoT22I	ATGCAT	2 cut(s)	617, 1671
EcoT38I	GRGCYC	4 cut(s)	141, 424, 723, 2046
ErhI	CCWWGG	2 cut(s)	2818, 3031
FaqI	GGGAC	5 cut(s)	239, 379, 586, 883, 2952
FauI	CCCGC	2 cut(s)	563, 1401
FbaI	TGATCA	4 cut(s)	379, 694, 2020, 2512
FblI	GTMKAC	1 cut(s)	2721
Fnu4HI	GCNGC	6 cut(s)	1068, 1100, 1619, 2071, 2613, 2714
FriOI	GRGCYC	4 cut(s)	141, 424, 723, 2046
Fsp4HI	GCNGC	6 cut(s)	1068, 1100, 1619, 2071, 2613, 2714
GlaI	GCGC	2 cut(s)	1283, 1662
GluI	GCNGC	6 cut(s)	1068, 1100, 1619, 2071, 2613, 2714
GsaI	CCCAGC	1 cut(s)	2831
GsuI	CTGGAG	3 cut(s)	1521, 1667, 2778
HaeII	RGCGCY	1 cut(s)	1664
HaeIII	GGCC	3 cut(s)	1688, 2184, 2817
HapII	CCGG	3 cut(s)	374, 1987, 2748
HhaI	GCGC	2 cut(s)	1284, 1663
Hin6I	GCGC	2 cut(s)	1282, 1661
HinP1I	GCGC	2 cut(s)	1282, 1661
HindIII	AAGCTT	3 cut(s)	734, 1439, 2195
HpaII	CCGG	3 cut(s)	374, 1987, 2748
Hpy166II	GTNNAC	7 cut(s)	682, 715, 1904, 2115, 2502, 2722, 2782
Hpy8I	GTNNAC	7 cut(s)	682, 715, 1904, 2115, 2502, 2722, 2782
HpyCH4IV	ACGT	3 cut(s)	1757, 2262, 2655
HpyF10VI	GCNNNNNNNGC	9 cut(s)	500, 521, 647, 1067, 1076, 1414, 2334, 2633, 2930
HpySE526I	ACGT	3 cut(s)	1757, 2262, 2655
HspAI	GCGC	2 cut(s)	1282, 1661
Kpn2I	TCCGGA	1 cut(s)	1986
Ksp22I	TGATCA	4 cut(s)	379, 694, 2020, 2512
LmnI	GCTCC	4 cut(s)	84, 204, 419, 2797
Lsp1109I	GCAGC	6 cut(s)	1054, 1086, 1630, 2057, 2624, 2725
LweI	GCATC	7 cut(s)	166, 304, 357, 481, 1048, 1948, 3064
MaeII	ACGT	3 cut(s)	1757, 2262, 2655
MaeIII	GTNAC	6 cut(s)	1231, 1532, 1702, 2137, 2994, 3152
MbiI	CCGCTC	2 cut(s)	570, 1408
MfeI	CAATTG	4 cut(s)	690, 900, 912, 1071
MflI	RGATCY	6 cut(s)	93, 1048, 1804, 2249, 2452, 2638
MhlI	GDGCHC	6 cut(s)	89, 141, 424, 723, 2046, 2089
MlyI	GAGTC	3 cut(s)	79, 895, 1999
MmeI	TCCRAC	2 cut(s)	1894, 2673
Mph1103I	ATGCAT	2 cut(s)	617, 1671
MroI	TCCGGA	1 cut(s)	1986
MseI	TTAA	8 cut(s)	167, 732, 1044, 1202, 1754, 2153, 2360, 2687
MslI	CAYNNNNRTG	2 cut(s)	1149, 2649
MspA1I	CMGCKG	3 cut(s)	1631, 1834, 2831
MspI	CCGG	3 cut(s)	374, 1987, 2748
MspR9I	CCNGG	7 cut(s)	374, 867, 1500, 1583, 1685, 2807, 3061
MunI	CAATTG	4 cut(s)	690, 900, 912, 1071
MvaI	CCWGG	6 cut(s)	867, 1500, 1583, 1685, 2807, 3061
MwoI	GCNNNNNNNGC	9 cut(s)	500, 521, 647, 1067, 1076, 1414, 2334, 2633, 2930
NciI	CCSGG	1 cut(s)	374
NcoI	CCATGG	1 cut(s)	2818
NlaIV	GGNNCC	7 cut(s)	200, 255, 808, 849, 1277, 1356, 1605
NmuCI	GTSAC	3 cut(s)	1532, 2137, 3152
NsiI	ATGCAT	2 cut(s)	617, 1671
NspI	RCATGY	5 cut(s)	507, 1148, 2358, 2508, 2525
PaeI	GCATGC	1 cut(s)	507
PaeR7I	CTCGAG	1 cut(s)	2999
PagI	TCATGA	1 cut(s)	3115
PceI	AGGCCT	1 cut(s)	1688
PciI	ACATGT	1 cut(s)	2504
PfeI	GAWTC	9 cut(s)	632, 896, 1082, 1244, 1448, 1850, 2171, 2366, 3041
PflMI	CCANNNNNTGG	1 cut(s)	1021
PkrI	GCNGC	6 cut(s)	1069, 1101, 1620, 2072, 2614, 2715
PleI	GAGTC	3 cut(s)	78, 894, 1998
PpsI	GAGTC	3 cut(s)	78, 894, 1998
PpuMI	RGGWCCY	1 cut(s)	253
PscI	ACATGT	1 cut(s)	2504
PshAI	GACNNNNGTC	1 cut(s)	1889
Psp124BI	GAGCTC	4 cut(s)	141, 424, 723, 2046
Psp1406I	AACGTT	1 cut(s)	2262
Psp5II	RGGWCCY	1 cut(s)	253
Psp6I	CCWGG	6 cut(s)	865, 1498, 1581, 1683, 2805, 3059
PspFI	CCCAGC	1 cut(s)	2827
PspGI	CCWGG	6 cut(s)	865, 1498, 1581, 1683, 2805, 3059
PspN4I	GGNNCC	7 cut(s)	200, 255, 808, 849, 1277, 1356, 1605
PspPI	GGNCC	4 cut(s)	253, 682, 2183, 2695
PspPPI	RGGWCCY	1 cut(s)	253
PspXI	VCTCGAGB	1 cut(s)	2999
PstI	CTGCAG	4 cut(s)	1104, 1620, 2122, 2339
PstNI	CAGNNNCTG	3 cut(s)	223, 2126, 2497
PsuI	RGATCY	6 cut(s)	93, 1048, 1804, 2249, 2452, 2638
PvuII	CAGCTG	3 cut(s)	1631, 1834, 2831
RsaI	GTAC	2 cut(s)	1117, 2503
RsaNI	GTAC	2 cut(s)	1116, 2502
RseI	CAYNNNNRTG	2 cut(s)	1149, 2649
SacI	GAGCTC	4 cut(s)	141, 424, 723, 2046
SaqAI	TTAA	8 cut(s)	167, 732, 1044, 1202, 1754, 2153, 2360, 2687
SatI	GCNGC	6 cut(s)	1068, 1100, 1619, 2071, 2613, 2714
Sau96I	GGNCC	4 cut(s)	253, 682, 2183, 2695
SchI	GAGTC	3 cut(s)	79, 895, 1999
ScrFI	CCNGG	7 cut(s)	374, 867, 1500, 1583, 1685, 2807, 3061
SduI	GDGCHC	6 cut(s)	89, 141, 424, 723, 2046, 2089
SfaNI	GCATC	7 cut(s)	166, 304, 357, 481, 1048, 1948, 3064
SfcI	CTRYAG	6 cut(s)	1100, 1616, 2118, 2335, 2662, 2870
Sfr274I	CTCGAG	1 cut(s)	2999
SinI	GGWCC	3 cut(s)	253, 682, 2695
SlaI	CTCGAG	1 cut(s)	2999
SmiMI	CAYNNNNRTG	2 cut(s)	1149, 2649
SmlI	CTYRAG	2 cut(s)	1218, 2999
SmoI	CTYRAG	2 cut(s)	1218, 2999
SphI	GCATGC	1 cut(s)	507
SseBI	AGGCCT	1 cut(s)	1688
SsiI	CCGC	4 cut(s)	277, 570, 1408, 2954
SstI	GAGCTC	4 cut(s)	141, 424, 723, 2046
StuI	AGGCCT	1 cut(s)	1688
StyD4I	CCNGG	7 cut(s)	372, 865, 1498, 1581, 1683, 2805, 3059
StyI	CCWWGG	2 cut(s)	2818, 3031
TaiI	ACGT	3 cut(s)	1760, 2265, 2658
TaqI	TCGA	8 cut(s)	498, 598, 723, 1795, 2567, 3000, 3193, 3210
TatI	WGTACW	1 cut(s)	2501
TfiI	GAWTC	9 cut(s)	632, 896, 1082, 1244, 1448, 1850, 2171, 2366, 3041
Tru1I	TTAA	8 cut(s)	167, 732, 1044, 1202, 1754, 2153, 2360, 2687
Tru9I	TTAA	8 cut(s)	167, 732, 1044, 1202, 1754, 2153, 2360, 2687
TseFI	GTSAC	3 cut(s)	1532, 2137, 3152
TseI	GCWGC	6 cut(s)	1067, 1099, 1618, 2070, 2612, 2713
Tsp45I	GTSAC	3 cut(s)	1532, 2137, 3152
TspDTI	ATGAA	6 cut(s)	201, 1122, 2604, 2854, 3024, 3054
TspGWI	ACGGA	5 cut(s)	191, 1159, 1733, 2075, 2547
Van91I	CCANNNNNTGG	1 cut(s)	1021
VpaK11BI	GGWCC	3 cut(s)	253, 682, 2695
XbaI	TCTAGA	1 cut(s)	1333
XceI	RCATGY	5 cut(s)	507, 1148, 2358, 2508, 2525
XcmI	CCANNNNNNNNNTGG	4 cut(s)	359, 840, 2835, 2892
XhoI	CTCGAG	1 cut(s)	2999
XmiI	GTMKAC	1 cut(s)	2721
Zsp2I	ATGCAT	2 cut(s)	617, 1671

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.