Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0006775

Physical Location & Seq

Genomic Coordinates Forward (+)

4814844 .. 4821303

Gene Structure 6460 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000016219

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3237 bp

ATGGGTTGCGTGTATTCGAGGGTCTGTATTGGAGAGGTTAGCTCAAGAGATGCGAGAAGGAAAGAGGCCCAGAATGCGAGGAACACTGAGATCCCAGTTTTCTCACCCAACTCTTCAAATGAGGAAGATGGTGAGATTGCGGATCAGTTCAACCAGGCAAACCATAACAGAGACGCTGAAATGGGGATCACTAGGCTGTCGAGGGTTTCGGCACAGTTTCTACCTCCAAATGGGTCGAGAACTGTGAAGGTTCCTTCTGGCAAGTTTGAGTTGAGGTACTCGTATTTGTCCCAGAGAGGGTATTACCCTGATGCTCTTGATAAGGCTAACCAGGACAGCTTTTGCATCCACACTCCCTTTGGGACGAACCCGGATGACCATTTTTTCGGGGTGTTTGATGGCCATGGAGAGTTTGGAACCGAGTGTTCTCAGTTTGTCAAGAGGAAGTTGTGCGAGAATTTGCTTAGGCATGGTAAGTTTCAAGTGGATGCTGTCGAGGCGTGTCATGAGGCGTTTCTTGCCACCAATTCGCAGTTGCATGATGACATTGTGGATGATACCATGAGTGGAACAACGGCCATTACGGTTTTGGTTAGAGGTAGGAAGATATACATTGCCAATTCAGGGGATTCTAGGGCTGTTATAGCCGAGAGGAAAGGGGAGGAGTTGGTGGCGGTGGATCTTTCCATTGATCAGACCCCGTTTAGGGTGGATGAGCTCGAAAGGGTTAAGATTTGTGGTGCAAGAGTGCTTACATTGGATCAGATTGAAGGGCTGAAGGATCCAGATGTGCAGTGTTGGGGCACCGAAGAAGGTGATGATGGTGATCCGCCTAGGCTCTGGGTGCCAAATGGAATGTATCCTGGGACCGCTTTTACGAGGAGTATTGGTGATTCCATTGCCGAGTCAATTGGAGTTGTTGCCAACCCTGAAATTGTGGTTTTGGAGCTTACACAGAATCATCCTTTCTTCGTGCTTGCTAGTGATGGAGTGTTTGAGTTCATTTCTAGCCAAACCGTGGTTGATATGGTTGCAAAATATAAAGATCCACGTGATGCTTGTGCTGCAATTGTTGCCGAATCTTATAAACTCTGGCTGCAGTATGAAACCCGTACAGATGATATTACAGTGATCGTGGTGCATGTAGATGGGCTAACTGCTACTGCTGTTGGTCAATCAGTACATCCTGGTTCTGTTTTACGGCCACCTGTTCCTCAAGTTGTAGAGGTTACAGGATCAGAATCTCCTTCAACCATTAGCTGGAACTCAAGGAACCCTCGCATAAAACATGATTTGTCAAAGGCACGTCTTCGTGTGATTGAAAATTCTCTAGAAAATGGGCAAGTTTGGGTTCCTCCGCCTCCAGCCCACAGAAAAACCTGGGAGGAAGAAGCACAAATTGAGCGAGCTTTACATGATCATTTCCTCTTCAGAAAACTTACCGACTCTCAGTGTCATGTTTTATTGGATTGCATGCAAAGGGTTGAGGTCCAGCCTGGAGACATTGTTGTTAGACAGGGTGGTGAAGGTGACTGCTTTTATGTTGTTGGCAGTGGAGAATTTGAGGTGTCGGCAACCCAGGAAGAAAAGAATGGAGAGGTTCCGAGGGTTTTGCAACGATACACAGCTGACAAACTGTCATCCTTTGGAGAACTAGCACTAATGTATAACAAACCACTCCAGGCATCCGTTCGTGCTGTCACCACTGGAACTCTCTGGGCTTTAAGAAGAGAAGACTTTCGTGGAATTCTAATGTCGGAGTTTTCTAATTTGTCATATTTGAAGTTGCTTCGATCAGTGGATCTTTTATCTAGGTTGACAATATTACAGCTCAGCCATATTGCTGATTCTCTTTCTGAGGTTTCATTCTCTGATGGGCAAACAATTGTCAATGAGAATGAAGGCCTATTGGCATTGTACATTATCCAGAAGGGAAAAGTGAGGATTACAGTTGACGCAAGTTCAGTTAGTAATCCAGTTGTCCGCAGTCTCGTGTCTGACAATCAAAAACAAGGTGATAATTCTCAGAGTGGTAAAGAGGTCATAGTGGAGAAGACAGAGGGAAGCTACTTTGGCGAATGGACACTTCTCGGGGAACATATTGATTTGTTTAATGCAGTTGCTGTGGGGGATGTTGTTTGTGCTGTTTTGACGAAGGAAAAATTTGATTCAGTCATTGGTCCAATGACAAAGCTTACTCAGGATGATCAGAAATCAAGGGATCACTCTTCGGAAACTTTAATGGAATCTGTTAAAAGTATTGATATCTCTGCTCTTACTAAAGTTCAGCTTGCTGATATGGAGTGGAGAAAGTGTCTGTATTCTACTGACTGCAGTGAGATCGGGCTTGTTCTTTTAAGAGATCCAGAAAATTTGCTTAGTTTGAAAAGGTTTTCAAAGAAGAAGGTCAGAAAGCTTGGAAAGGAAGCACAAGTATTAAAAGAGAAGGATCTAATCAAGAGCATAAGCCCTTCAGCTTGTGTGCCACAGGTTCTATCCACTTGTGTTGATCAGACACATGCTGCAATACTGCTCAATACATGCATTGCTTGTCCATTGGCCTCGATACTTCTCACGCCACTTGATGAACCATCTGCCCAATTTTGTACAGCCTCTCTAATTACTGCTTTGGAGGATCTCCACAAGAATGACGTTCTCTACAGAGGCTTGTCTCCTGATGCATTAATGTTGGACCACACAGGACATTTACAGCTTGTAGACTTCAGATTCGGGGAAAAGTTGTCTGGCCAAAGAACATATACAATTTGTGGAATGGCAGACTTTTTAGCTCCAGAGGTAGTTCAGGGAATAGGCCATGGTTTCCCAGCTGACTGGTGGGCGTTGGGAGTCTTGATATATTTCATGTTACAAGGTGAATTGCCTTTTGGGTCATGGAGAGAAAGTGAGCTTGATATCTTTGCAAAGATTGCGAAGGGACAGCTTAATCTTCCTCAAACTTTCAGCCCTGGAGTTGTTGATCTCATTACCAAGTTACTTGAGGTTGATGAAAACACCAGACTAGGAAGCCAAGGTTGTGATTCTGTTAAAAGTCATCCCTGGTTCGATGGTGTTGATTGGAAAGGGATTAGAGACTCTAGTTTTCCTGTTCCTCAGGAGATCACCTCTCGCATAGCTCAACATTTGGAAAGTCACTCAGACGAGTATAGTGCTCCTCAAGGTTCTCCGTCACACAATGAAGAAGAACGTGACATTCCGGAATGGTTTGATGACTGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko04022 ko04540 ko04611 ko04713 ko04714 ko04730 ko04740 ko04923 ko04924 ko04970 map04022 map04540 map04611 map04713 map04714 map04730 map04740 map04923 map04924 map04970

Pfam Domains

Protein Families

PP2C Pkinase cNMP_binding

Protein Analysis

1079

Amino Acids

119.67

Weight (kDa)

5.02

Isoelectric Point (pI)

37.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PP2C	PF00481	118 - 360	8.1e-34	Protein phosphatase 2C
cNMP_binding	PF00027	495 - 586	8.7e-16	Cyclic nucleotide-binding domain
Pkinase	PF00069	787 - 1021	4.3e-35	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	808 - 1007	3.4e-08	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0006775)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G20050 AT2G20050
fragaria_vesca	FvH4_1g05740 FvH4_1g05740 FvH4_1g05740
malus_domestica	MD02G1058500.v1.1 MD15G1192800.v1.1
prunus_persica	Prupe.7G224500_v2.0.a1 Prupe.7G224500_v2.0.a1 Prupe.7G224500_v2.0.a1 Prupe.7G224500_v2.0.a1
pyrus_communis	pycom02g04740 pycom15g17250 pycom15g17260
rosa_chinensis	RchiOBHm_Chr2g0091381
rosa_laevigata	RLG00000016219
rosa_multiflora	Rmu_ssc0000333.1_g000004 Rmu_ssc0000333.1_g000011
rosa_roxburghii	Rroxscaffold_2G00150130
rosa_rugosa	Rorug02G0017200 Rorug02G0017300
rosa_samantha	Rh2AG062300 Rh2BG061300 Rh2CG063400 Rh2DG061900
rosa_wichuraiana	Rw2G005790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1086
AccB1I	GGYRCC	2 cut(s)	803, 844
AccB7I	CCANNNNNTGG	2 cut(s)	1018, 1260
AccI	GTMKAC	1 cut(s)	2718
AccIII	TCCGGA	1 cut(s)	3214
AciI	CCGC	6 cut(s)	140, 674, 830, 870, 1358, 1984
AcoI	YGGCCR	4 cut(s)	400, 576, 1202, 2746
AcsI	RAATTY	6 cut(s)	457, 1324, 1559, 1746, 2162, 2371
AcuI	CTGAAG	4 cut(s)	797, 1414, 2457, 2707
AcvI	CACGTG	1 cut(s)	1052
AfaI	GTAC	5 cut(s)	278, 1114, 1182, 1919, 2608
AfiI	CCNNNNNNNGG	7 cut(s)	230, 297, 624, 705, 706, 1018, 1260
AgsI	TTSAA	9 cut(s)	117, 151, 482, 770, 1251, 1322, 1783, 2386, 2397
AhdI	GACNNNNNGTC	1 cut(s)	1636
AjiI	CACGTC	1 cut(s)	1307
AjuI	GAANNNNNNNTTGG	2 cut(s)	2868, 2900
Alw21I	GWGCWC	2 cut(s)	720, 3172
Alw26I	GTCTC	5 cut(s)	165, 1494, 1994, 2676, 3084
AlwNI	CAGNNNCTG	1 cut(s)	2123
Ama87I	CYCGRG	1 cut(s)	2090
Aor13HI	TCCGGA	1 cut(s)	3214
AoxI	GGCC	8 cut(s)	66, 400, 576, 1202, 1903, 2559, 2746, 2812
ApeKI	GCWGC	3 cut(s)	1064, 1096, 2522
ApoI	RAATTY	6 cut(s)	457, 1324, 1559, 1746, 2162, 2371
ArsI	GACNNNNNNTTYG	4 cut(s)	368, 400, 2711, 2743
AseI	ATTAAT	1 cut(s)	2684
Asp700I	GAANNNNTTC	1 cut(s)	1737
AspA2I	CCTAGG	1 cut(s)	833
AspS9I	GGNCC	5 cut(s)	67, 867, 1489, 2180, 2692
AsuC2I	CCSGG	1 cut(s)	371
AvaI	CYCGRG	1 cut(s)	2090
AvaII	GGWCC	4 cut(s)	867, 1489, 2180, 2692
AvrII	CCTAGG	1 cut(s)	833
AxyI	CCTNAGG	1 cut(s)	3111
BaeGI	GKGCMC	1 cut(s)	806
BalI	TGGCCA	2 cut(s)	402, 2748
BamHI	GGATCC	1 cut(s)	781
BanI	GGYRCC	2 cut(s)	803, 844
BanII	GRGCYC	1 cut(s)	720
BauI	CACGAG	1 cut(s)	1991
BbrPI	CACGTG	1 cut(s)	1052
BbsI	GAAGAC	3 cut(s)	1301, 1740, 2060
Bbv12I	GWGCWC	2 cut(s)	720, 3172
BbvI	GCAGC	3 cut(s)	1051, 1083, 2509
BccI	CCATC	8 cut(s)	122, 392, 815, 980, 1142, 1868, 2599, 3059
BceAI	ACGGC	2 cut(s)	591, 1217
BcgI	CGANNNNNNTGC	6 cut(s)	1594, 1628, 2323, 2357, 3149, 3183
BciVI	GTATCC	1 cut(s)	870
BclI	TGATCA	4 cut(s)	691, 1417, 2206, 2509
BcnI	CCSGG	1 cut(s)	371
BcoDI	GTCTC	5 cut(s)	165, 1494, 1994, 2676, 3084
BfmI	CTRYAG	3 cut(s)	1097, 2332, 2659
BfuI	GTATCC	1 cut(s)	870
BisI	GCNGC	3 cut(s)	1065, 1097, 2523
BlnI	CCTAGG	1 cut(s)	833
BlpI	GCTNAGC	1 cut(s)	1832
BlsI	GCNGC	3 cut(s)	1066, 1098, 2524
Bme18I	GGWCC	4 cut(s)	867, 1489, 2180, 2692
BmeRI	GACNNNNNGTC	1 cut(s)	1636
BmeT110I	CYCGRG	1 cut(s)	2090
BmgBI	CACGTC	1 cut(s)	1307
BmgT120I	GGNCC	5 cut(s)	67, 867, 1489, 2180, 2692
BmiI	GGNNCC	9 cut(s)	252, 418, 783, 805, 846, 868, 1274, 1353, 1602
BmrI	ACTGGG	1 cut(s)	89
BmsI	GCATC	7 cut(s)	40, 301, 354, 478, 1045, 1694, 2668
BmuI	ACTGGG	1 cut(s)	89
BpiI	GAAGAC	3 cut(s)	1301, 1740, 2060
BplI	GAGNNNNNCTC	4 cut(s)	26, 58, 3107, 3139
BpmI	CTGGAG	5 cut(s)	1347, 1518, 1664, 2775, 2988
Bpu10I	CCTNAGC	1 cut(s)	464
Bpu1102I	GCTNAGC	1 cut(s)	1832
BpuEI	CTTGAG	5 cut(s)	28, 1200, 1252, 3017, 3159
BpuMI	CCSGG	1 cut(s)	371
BsaAI	YACGTR	1 cut(s)	1052
BsaBI	GATNNNNATC	2 cut(s)	825, 1240
BsaWI	WCCGGW	1 cut(s)	3214
BsaXI	ACNNNNNCTCC	8 cut(s)	874, 904, 1491, 1521, 2299, 2329, 2887, 2917
Bsc4I	CCNNNNNNNGG	7 cut(s)	230, 297, 624, 705, 706, 1018, 1260
Bse1I	ACTGG	5 cut(s)	95, 1711, 1976, 2837, 3236
Bse21I	CCTNAGG	1 cut(s)	3111
Bse3DI	GCAATG	3 cut(s)	612, 897, 2544
Bse8I	GATNNNNATC	2 cut(s)	825, 1240
BseAI	TCCGGA	1 cut(s)	3214
BseJI	GATNNNNATC	2 cut(s)	825, 1240
BseLI	CCNNNNNNNGG	7 cut(s)	230, 297, 624, 705, 706, 1018, 1260
BseMI	GCAATG	3 cut(s)	612, 897, 2544
BseMII	CTCAG	9 cut(s)	78, 443, 1463, 1846, 1848, 2039, 2213, 3125, 3168
BseNI	ACTGG	5 cut(s)	95, 1711, 1976, 2837, 3236
BseRI	GAGGAG	3 cut(s)	677, 895, 3162
BseSI	GKGCMC	1 cut(s)	806
BseXI	GCAGC	3 cut(s)	1051, 1083, 2509
BseYI	CCCAGC	1 cut(s)	2824
BsgI	GTGCAG	1 cut(s)	812
BshFI	GGCC	8 cut(s)	68, 402, 578, 1204, 1905, 2561, 2748, 2814
BshNI	GGYRCC	2 cut(s)	803, 844
BsiHKAI	GWGCWC	2 cut(s)	720, 3172
BsiHKCI	CYCGRG	1 cut(s)	2090
BsiSI	CCGG	2 cut(s)	371, 3215
BslFI	GGGAC	4 cut(s)	274, 376, 880, 2949
BslI	CCNNNNNNNGG	7 cut(s)	230, 297, 624, 705, 706, 1018, 1260
BsmAI	GTCTC	5 cut(s)	165, 1494, 1994, 2676, 3084
BsmBI	CGTCTC	1 cut(s)	165
BsmFI	GGGAC	4 cut(s)	274, 376, 880, 2949
BsmI	GAATGC	1 cut(s)	79
BsnI	GGCC	8 cut(s)	68, 402, 578, 1204, 1905, 2561, 2748, 2814
BsoBI	CYCGRG	1 cut(s)	2090
Bsp1286I	GDGCHC	3 cut(s)	720, 806, 3172
Bsp13I	TCCGGA	1 cut(s)	3214
Bsp1407I	TGTACA	2 cut(s)	1917, 2606
Bsp1720I	GCTNAGC	1 cut(s)	1832
Bsp19I	CCATGG	2 cut(s)	403, 2815
BspACI	CCGC	6 cut(s)	140, 674, 830, 870, 1358, 1984
BspANI	GGCC	8 cut(s)	68, 402, 578, 1204, 1905, 2561, 2748, 2814
BspCNI	CTCAG	9 cut(s)	79, 442, 1462, 1845, 1849, 2038, 2212, 3124, 3167
BspEI	TCCGGA	1 cut(s)	3214
BspHI	TCATGA	1 cut(s)	505
BspLI	GGNNCC	9 cut(s)	252, 418, 783, 805, 846, 868, 1274, 1353, 1602
BspMAI	CTGCAG	2 cut(s)	1101, 2336
BspT107I	GGYRCC	2 cut(s)	803, 844
BsrDI	GCAATG	3 cut(s)	612, 897, 2544
BsrGI	TGTACA	2 cut(s)	1917, 2606
BsrI	ACTGG	5 cut(s)	95, 1711, 1976, 2837, 3236
BssSI	CACGAG	1 cut(s)	1991
BssT1I	CCWWGG	4 cut(s)	403, 833, 2815, 3028
Bst2BI	CACGAG	1 cut(s)	1991
Bst4CI	ACNGT	7 cut(s)	216, 244, 586, 1018, 1129, 1638, 1951
Bst6I	CTCTTC	4 cut(s)	118, 1433, 1723, 2233
BstAPI	GCANNNNNTGC	2 cut(s)	1073, 2927
BstAUI	TGTACA	2 cut(s)	1917, 2606
BstBAI	YACGTR	1 cut(s)	1052
BstC8I	GCNNGC	4 cut(s)	978, 1407, 1475, 2292
BstDSI	CCRYGG	3 cut(s)	403, 1017, 2815
BstMAI	GTCTC	5 cut(s)	165, 1494, 1994, 2676, 3084
BstMWI	GCNNNNNNNGC	9 cut(s)	74, 497, 518, 644, 844, 1064, 1073, 2073, 2927
BstNSI	RCATGY	4 cut(s)	1145, 1477, 2522, 2544
BstSFI	CTRYAG	3 cut(s)	1097, 2332, 2659
BstSLI	GKGCMC	1 cut(s)	806
BstV1I	GCAGC	3 cut(s)	1051, 1083, 2509
BstV2I	GAAGAC	3 cut(s)	1301, 1740, 2060
BstX2I	RGATCY	8 cut(s)	90, 679, 781, 1045, 1801, 2362, 2449, 2635
BstXI	CCANNNNNNTGG	1 cut(s)	2832
BstYI	RGATCY	8 cut(s)	90, 679, 781, 1045, 1801, 2362, 2449, 2635
Bsu36I	CCTNAGG	1 cut(s)	3111
BsuI	GTATCC	1 cut(s)	870
BsuRI	GGCC	8 cut(s)	68, 402, 578, 1204, 1905, 2561, 2748, 2814
BtgI	CCRYGG	3 cut(s)	403, 1017, 2815
BtrI	CACGTC	1 cut(s)	1307
BtsI	GCAGTG	3 cut(s)	800, 1558, 2341
BtsIMutI	CAGTG	8 cut(s)	84, 800, 1134, 1457, 1558, 1704, 1803, 2341
Cac8I	GCNNGC	4 cut(s)	978, 1407, 1475, 2292
CaiI	CAGNNNCTG	1 cut(s)	2123
CciI	TCATGA	1 cut(s)	505
Cfr13I	GGNCC	5 cut(s)	67, 867, 1489, 2180, 2692
CseI	GACGC	2 cut(s)	182, 1964
Csp6I	GTAC	5 cut(s)	277, 1113, 1181, 1918, 2607
CspCI	CAANNNNNGTGG	2 cut(s)	2488, 2523
CviQI	GTAC	5 cut(s)	277, 1113, 1181, 1918, 2607
DriI	GACNNNNNGTC	1 cut(s)	1636
EaeI	YGGCCR	4 cut(s)	400, 576, 1202, 2746
Eam1104I	CTCTTC	4 cut(s)	118, 1433, 1723, 2233
Eam1105I	GACNNNNNGTC	1 cut(s)	1636
EarI	CTCTTC	4 cut(s)	118, 1433, 1723, 2233
EciI	GGCGGA	2 cut(s)	819, 1347
Ecl136II	GAGCTC	1 cut(s)	718
Eco130I	CCWWGG	4 cut(s)	403, 833, 2815, 3028
Eco147I	AGGCCT	1 cut(s)	1905
Eco24I	GRGCYC	1 cut(s)	720
Eco32I	GATATC	2 cut(s)	2266, 2914
Eco47I	GGWCC	4 cut(s)	867, 1489, 2180, 2692
Eco53kI	GAGCTC	1 cut(s)	718
Eco57I	CTGAAG	4 cut(s)	797, 1414, 2457, 2707
Eco72I	CACGTG	1 cut(s)	1052
Eco81I	CCTNAGG	1 cut(s)	3111
Eco88I	CYCGRG	1 cut(s)	2090
EcoICRI	GAGCTC	1 cut(s)	718
EcoRI	GAATTC	1 cut(s)	1746
EcoRV	GATATC	2 cut(s)	2266, 2914
EcoT14I	CCWWGG	4 cut(s)	403, 833, 2815, 3028
EcoT22I	ATGCAT	2 cut(s)	2546, 2683
EcoT38I	GRGCYC	1 cut(s)	720
ErhI	CCWWGG	4 cut(s)	403, 833, 2815, 3028
Esp3I	CGTCTC	1 cut(s)	165
FalI	AAGNNNNNCTT	2 cut(s)	2274, 2306
FaqI	GGGAC	4 cut(s)	274, 376, 880, 2949
FbaI	TGATCA	4 cut(s)	691, 1417, 2206, 2509
FblI	GTMKAC	1 cut(s)	2718
Fnu4HI	GCNGC	3 cut(s)	1065, 1097, 2523
FriOI	GRGCYC	1 cut(s)	720
Fsp4HI	GCNGC	3 cut(s)	1065, 1097, 2523
GluI	GCNGC	3 cut(s)	1065, 1097, 2523
GsaI	CCCAGC	1 cut(s)	2828
GsuI	CTGGAG	5 cut(s)	1347, 1518, 1664, 2775, 2988
HaeIII	GGCC	8 cut(s)	68, 402, 578, 1204, 1905, 2561, 2748, 2814
HapII	CCGG	2 cut(s)	371, 3215
HgaI	GACGC	2 cut(s)	182, 1964
HincII	GTYRAC	2 cut(s)	1818, 1954
HindII	GTYRAC	2 cut(s)	1818, 1954
HindIII	AAGCTT	2 cut(s)	2192, 2414
HpaII	CCGG	2 cut(s)	371, 3215
Hpy166II	GTNNAC	3 cut(s)	1818, 1954, 2719
Hpy8I	GTNNAC	3 cut(s)	1818, 1954, 2719
HpyCH4III	ACNGT	7 cut(s)	216, 244, 586, 1018, 1129, 1638, 1951
HpyCH4IV	ACGT	4 cut(s)	1051, 1306, 2652, 3205
HpyF10VI	GCNNNNNNNGC	9 cut(s)	74, 497, 518, 644, 844, 1064, 1073, 2073, 2927
HpySE526I	ACGT	4 cut(s)	1051, 1306, 2652, 3205
Kpn2I	TCCGGA	1 cut(s)	3214
Ksp22I	TGATCA	4 cut(s)	691, 1417, 2206, 2509
LmnI	GCTCC	3 cut(s)	946, 2794, 3175
Lsp1109I	GCAGC	3 cut(s)	1051, 1083, 2509
LweI	GCATC	7 cut(s)	40, 301, 354, 478, 1045, 1694, 2668
MaeII	ACGT	4 cut(s)	1051, 1306, 2652, 3205
MaeIII	GTNAC	8 cut(s)	1228, 1529, 1699, 2865, 2991, 3149, 3186, 3206
MfeI	CAATTG	3 cut(s)	909, 1068, 1884
MflI	RGATCY	8 cut(s)	90, 679, 781, 1045, 1801, 2362, 2449, 2635
MhlI	GDGCHC	3 cut(s)	720, 806, 3172
MlsI	TGGCCA	2 cut(s)	402, 2748
MluNI	TGGCCA	2 cut(s)	402, 2748
MlyI	GAGTC	4 cut(s)	914, 1439, 2856, 3086
MmeI	TCCRAC	2 cut(s)	1737, 2670
Mox20I	TGGCCA	2 cut(s)	402, 2748
Mph1103I	ATGCAT	2 cut(s)	2546, 2683
MroI	TCCGGA	1 cut(s)	3214
MroXI	GAANNNNTTC	1 cut(s)	1737
MscI	TGGCCA	2 cut(s)	402, 2748
MslI	CAYNNNNRTG	2 cut(s)	1146, 2646
Msp20I	TGGCCA	2 cut(s)	402, 2748
MspA1I	CMGCKG	2 cut(s)	1628, 2828
MspI	CCGG	2 cut(s)	371, 3215
MunI	CAATTG	3 cut(s)	909, 1068, 1884
Mva1269I	GAATGC	1 cut(s)	79
MwoI	GCNNNNNNNGC	9 cut(s)	74, 497, 518, 644, 844, 1064, 1073, 2073, 2927
NciI	CCSGG	1 cut(s)	371
NcoI	CCATGG	2 cut(s)	403, 2815
NlaIV	GGNNCC	9 cut(s)	252, 418, 783, 805, 846, 868, 1274, 1353, 1602
NmeAIII	GCCGAG	2 cut(s)	673, 928
NmuCI	GTSAC	5 cut(s)	1529, 1699, 3149, 3186, 3206
NsiI	ATGCAT	2 cut(s)	2546, 2683
NspI	RCATGY	4 cut(s)	1145, 1477, 2522, 2544
PaeI	GCATGC	1 cut(s)	1477
PagI	TCATGA	1 cut(s)	505
PceI	AGGCCT	1 cut(s)	1905
PctI	GAATGC	1 cut(s)	79
PdmI	GAANNNNTTC	1 cut(s)	1737
PflMI	CCANNNNNTGG	2 cut(s)	1018, 1260
PkrI	GCNGC	3 cut(s)	1066, 1098, 2524
PleI	GAGTC	4 cut(s)	913, 1439, 2855, 3086
PmaCI	CACGTG	1 cut(s)	1052
PmlI	CACGTG	1 cut(s)	1052
PpsI	GAGTC	4 cut(s)	913, 1439, 2855, 3086
Ppu21I	YACGTR	1 cut(s)	1052
PshBI	ATTAAT	1 cut(s)	2684
PsiI	TTATAA	1 cut(s)	1086
Psp124BI	GAGCTC	1 cut(s)	720
PspCI	CACGTG	1 cut(s)	1052
PspFI	CCCAGC	1 cut(s)	2824
PspN4I	GGNNCC	9 cut(s)	252, 418, 783, 805, 846, 868, 1274, 1353, 1602
PspPI	GGNCC	5 cut(s)	67, 867, 1489, 2180, 2692
PstI	CTGCAG	2 cut(s)	1101, 2336
PstNI	CAGNNNCTG	1 cut(s)	2123
PsuI	RGATCY	8 cut(s)	90, 679, 781, 1045, 1801, 2362, 2449, 2635
PvuII	CAGCTG	2 cut(s)	1628, 2828
RsaI	GTAC	5 cut(s)	278, 1114, 1182, 1919, 2608
RsaNI	GTAC	5 cut(s)	277, 1113, 1181, 1918, 2607
RseI	CAYNNNNRTG	2 cut(s)	1146, 2646
SacI	GAGCTC	1 cut(s)	720
SatI	GCNGC	3 cut(s)	1065, 1097, 2523
Sau96I	GGNCC	5 cut(s)	67, 867, 1489, 2180, 2692
SchI	GAGTC	4 cut(s)	914, 1439, 2856, 3086
SduI	GDGCHC	3 cut(s)	720, 806, 3172
SfaNI	GCATC	7 cut(s)	40, 301, 354, 478, 1045, 1694, 2668
SfcI	CTRYAG	3 cut(s)	1097, 2332, 2659
SinI	GGWCC	4 cut(s)	867, 1489, 2180, 2692
SmiMI	CAYNNNNRTG	2 cut(s)	1146, 2646
SmlI	CTYRAG	5 cut(s)	43, 1215, 1267, 2996, 3174
SmoI	CTYRAG	5 cut(s)	43, 1215, 1267, 2996, 3174
SphI	GCATGC	1 cut(s)	1477
SseBI	AGGCCT	1 cut(s)	1905
SsiI	CCGC	6 cut(s)	140, 674, 830, 870, 1358, 1984
SspI	AATATT	1 cut(s)	1824
SstI	GAGCTC	1 cut(s)	720
StuI	AGGCCT	1 cut(s)	1905
StyI	CCWWGG	4 cut(s)	403, 833, 2815, 3028
TaaI	ACNGT	7 cut(s)	216, 244, 586, 1018, 1129, 1638, 1951
TaiI	ACGT	4 cut(s)	1054, 1309, 2655, 3208
TaqI	TCGA	8 cut(s)	17, 200, 236, 495, 720, 1792, 2564, 3063
TatI	WGTACW	3 cut(s)	1180, 1917, 2606
TscAI	CASTG	8 cut(s)	91, 800, 1134, 1457, 1558, 1711, 1803, 2341
TseFI	GTSAC	5 cut(s)	1529, 1699, 3149, 3186, 3206
TseI	GCWGC	3 cut(s)	1064, 1096, 2522
Tsp45I	GTSAC	5 cut(s)	1529, 1699, 3149, 3186, 3206
TspDTI	ATGAA	8 cut(s)	991, 1119, 1854, 1914, 2601, 2851, 3021, 3210
TspGWI	ACGGA	2 cut(s)	1678, 3174
TspRI	CASTG	8 cut(s)	91, 800, 1134, 1457, 1558, 1711, 1803, 2341
Van91I	CCANNNNNTGG	2 cut(s)	1018, 1260
VpaK11BI	GGWCC	4 cut(s)	867, 1489, 2180, 2692
VspI	ATTAAT	1 cut(s)	2684
XapI	RAATTY	6 cut(s)	457, 1324, 1559, 1746, 2162, 2371
XbaI	TCTAGA	1 cut(s)	1330
XceI	RCATGY	4 cut(s)	1145, 1477, 2522, 2544
XcmI	CCANNNNNNNNNTGG	4 cut(s)	356, 410, 586, 2832
XmaJI	CCTAGG	1 cut(s)	833
XmiI	GTMKAC	1 cut(s)	2718
XmnI	GAANNNNTTC	1 cut(s)	1737
Zsp2I	ATGCAT	2 cut(s)	2546, 2683

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.