Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2D

Orthogroup ID

OG0006775

Physical Location & Seq

Genomic Coordinates Forward (+)

4542036 .. 4549729

Gene Structure 7694 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2DG061900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2850 bp

ATGGGTTGCGTGTATTCGAGGGTCTGTATTGGAGAGGTTGGTAGTAGTAGTAGTTCAAGAGATGCGAGAAGGAGAGAGGCCCAGAATGCGAGGAACACTGAGATCCCAGTTTTCTCACCCAACTCTTCAAGTGAGGAAGATGGTGAGACTGGAGATCAGTTCAACCAGGCAAACCATACCAGAGACGCTGAAATGGGGATCACTAGGCTGTCGAGGGTTTCGGCACAGTTTCTACCTCCAAATGGGTCGAGAACTGTCAAGGTTCCCTCTGGCAAGTTTGAGTTGAGGTACTCGTATTTGTCCCAGAGAGGGTATTACCCTGATGCTCTCGATAAGGCCAACCAGGACAGCTTTTGCATCCACACTCCGTTTGGGACGAACCCGAATGACCATTTTTTCGGGGTGTTTGATGGCCATGGCGAGTTTGGAACCGAGTGTTCGCAGTTTGTCAAGAGGAAGTTGTGTGAGAATTTGCTTAGGCATGGTAAGTTTCAAGTGGATGCTGTCGAGGCGTGTCGCGAGGCGTTTCTTGCCACGAATTCGCAGTTGCATGATGACATTGTGGATGACAGCATGAGTGGGACAACAGCCATTACGGTTTTGGTTAGAGGTAGGAAGATATACATTGCCAATTCGGGGGATTCTAGAGCTGTTATAGCGGAGAGGAAAGGGGAGGAGTTGGTGGCGGTGGATCTTTCGATTGATCAGACCCCGTTCAGGGTGGATGAGCTCGAAAGGGTTAAGATTTGTGGTGCAAGAGTGCTCACATTGGATCAGATTGAAGGGCTGAAGAATCCTGATGTGCAGTGTTGGGGCACCGAAGAAGGTGATGATGGTGATCCGCCTAGGCTGTGGGTGCCGAATGGAATGTATCCTGGGACCGCTTTTACCAGGAGTATTGGTGATTCGATTGCTGAGTCAATTGGAGTTGTTGCCAACCCTGAAATTGTGGTTCTGGAGCTTACACAGAATCATCCTTTCTTCGTCCTTGCTAGTGATGGAGTGTTTGAGTTTATTTCTAGCCAAACCGTGGTTGATATGGTTGCAAAATATAAAGATCCACGTGATGCTTGTGCTGCAATTGTTGCCGAATCTTATAAACTCTGGCTGCAGTATGAAACCCGTACAGATGATATTACAGTGATCGTGGTGCATGTAGATGGGCTAACTGCTACTGCTGTTGGTCAATCAGTACATCCTGGTTCTGTTTTACGGCCACCTGTTCCTCAAGTTGTAGAGGTTACAGGATCAGAATCTCCTTCAACCATTAGCTGGAACTCAAGGAACCCTCGCATAAAACATGATTTGTCAAAGGCACGTCTTCGTGTGATTGAAAATTCTCTAGAAAACGGGCAAGTTTGGGTTCCTCCGCCTCCAGCCCACAGAAAAACCTGGGAGGAAGAGGCACAAATTGAGCGAGCTTTACATGATCATTTCCTCTTCAGAAAACTTACCGACTCTCAGTGTCATGTTTTATTGGATTGCATGCAAAGGGTTGAGGTCCAGCCTGGAGACATTGTTGTTAGACAGGGTGGTGAAGGTGACTGCTTTTATGTTGTTGGCAGTGGAGAATTTGAGGTGTCGGCAACCCAGGAAGAAAAGAACGGAGAGGTTCCGAGGGTTTTGCAACGATATACAGCTGACAAACTGTCATCCTTTGGAGAACTAGCACTAATGTATAACAAACCACTCCAGGCATCCGTTCGTGCTGTCACCACTGGAACTCTCTGGGCTTTAAGAAGAGAAGACTTTCGTGGAATTCTAATGTCAGAGTTTTCTAATTTGTCATATTTGAAGTTGCTTCGATCAGTGGATCTTTTATCCAGGTTGACAATATTACAGCTCAGCCATATTGCTGATTCTCTTTCTGAGGTTTCATTCTCAGATGGGCAAACAATTGTCAATGAGAATGAAGGCCTATTGGCATTGTACATTATCCAGAAGGGAAAAGTGAGGATTACAGTTGACGCAAGTTCAGTTAGCAATCCAGTTGTCTGCAGTCTCATGTCTGACAATCAAAAACAAGGTGATAATCCTCAGAGCGGTAAAGAGATCATAGTGGAGAAGACAGAGGGAAGCTACTTTGGCGAATGGACACTTCTCGGGGAACATATTGATTTGTTTAATGCAGTTGCTGTGGGGGATGTTGTTTGTGCTGTTTTGACAAAGGAAAAATTTGATTCAGTCATTGGCCCAATGACAAAGCTTACTCAGGATGATCAGAAATCAAGGGATCAATCATCGGAAACTTTAATGGAATCTGTTAAAAGTATTGATTTCTCTGCTCTTACTAAAGTTCAGCTTGCTGATATGGAGTGGAGAAAGTGTCTGTTTTCTACTGACTGCAGTGAGATTGGGCTTGTTCTTTTAAGAGATCCAGAGAATTTGCTTAGTTTGAAAAGGTTTTCAAAGAAGAAGGTCAGAAAGATTGGAAAGGAAGCACAAGTATTAAAAGAGAAGGATCTAATCAAGAGCATAAGCCCTTCAGCTTGTGTGCCACAGGTTCTATCCACTTGTGTTGATCAGACACATGCTGCAATACTGCTCAATACATGCATTGCTTGTCCATTGGCCTCGATACTTCGCACGCCCCTTGATGAACCGTCTGCCCAATTTTGTACAGCCTCTCTAATTACTGCTTTGGAGGATCTCCACAAGAATAACGTTCTCTACAGAGGCTTGTCTCCTGATGCATTAATGTTGGACCACAAGGGACATTTACAGATTGTAGACTTCAGATTTGGGAAAAAGTTGTCTGGCCAAAGAACATATACAATTTGTGGAATGGCAGACTTTTTAGCTCCAGAGGTAGTTCAGGGAATAGGCCATGGTTTCCCAGCTGACTGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko04022 ko04540 ko04611 ko04713 ko04714 ko04730 ko04740 ko04923 ko04924 ko04970 map04022 map04540 map04611 map04713 map04714 map04730 map04740 map04923 map04924 map04970

Pfam Domains

Protein Families

PP2C Pkinase cNMP_binding

Protein Analysis

949

Amino Acids

104.92

Weight (kDa)

5.32

Isoelectric Point (pI)

37.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PP2C	PF00481	122 - 364	1.2e-34	Protein phosphatase 2C
cNMP_binding	PF00027	499 - 590	7.3e-16	Cyclic nucleotide-binding domain
Pkinase	PF00069	790 - 949	8.7e-20	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0006775)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G20050 AT2G20050
fragaria_vesca	FvH4_1g05740 FvH4_1g05740 FvH4_1g05740
malus_domestica	MD02G1058500.v1.1 MD15G1192800.v1.1
prunus_persica	Prupe.7G224500_v2.0.a1 Prupe.7G224500_v2.0.a1 Prupe.7G224500_v2.0.a1 Prupe.7G224500_v2.0.a1
pyrus_communis	pycom02g04740 pycom15g17250 pycom15g17260
rosa_chinensis	RchiOBHm_Chr2g0091381
rosa_laevigata	RLG00000016219
rosa_multiflora	Rmu_ssc0000333.1_g000004 Rmu_ssc0000333.1_g000011
rosa_roxburghii	Rroxscaffold_2G00150130
rosa_rugosa	Rorug02G0017200 Rorug02G0017300
rosa_samantha	Rh2AG062300 Rh2BG061300 Rh2CG063400 Rh2DG061900
rosa_wichuraiana	Rw2G005790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1098
AccB1I	GGYRCC	2 cut(s)	815, 856
AccB7I	CCANNNNNTGG	2 cut(s)	1030, 1272
AccBSI	CCGCTC	1 cut(s)	2043
AccI	GTMKAC	1 cut(s)	2730
AccII	CGCG	1 cut(s)	519
AciI	CCGC	6 cut(s)	659, 686, 842, 882, 1370, 2043
AclI	AACGTT	1 cut(s)	2664
AcoI	YGGCCR	3 cut(s)	412, 1214, 2758
AcsI	RAATTY	7 cut(s)	469, 538, 1336, 1571, 1758, 2174, 2383
AcuI	CTGAAG	4 cut(s)	809, 1426, 2469, 2719
AcvI	CACGTG	1 cut(s)	1064
AfaI	GTAC	5 cut(s)	290, 1126, 1194, 1931, 2620
AfiI	CCNNNNNNNGG	8 cut(s)	242, 309, 636, 717, 718, 1030, 1272, 2042
AhdI	GACNNNNNGTC	1 cut(s)	1648
AjiI	CACGTC	1 cut(s)	1319
Alw21I	GWGCWC	2 cut(s)	732, 765
Alw26I	GTCTC	5 cut(s)	140, 177, 1506, 2006, 2688
AlwNI	CAGNNNCTG	1 cut(s)	2135
Ama87I	CYCGRG	1 cut(s)	2102
AoxI	GGCC	9 cut(s)	78, 336, 412, 1214, 1915, 2191, 2571, 2758, 2824
ApeKI	GCWGC	3 cut(s)	1076, 1108, 2534
ApoI	RAATTY	7 cut(s)	469, 538, 1336, 1571, 1758, 2174, 2383
ArsI	GACNNNNNNTTYG	4 cut(s)	380, 412, 2723, 2755
AseI	ATTAAT	1 cut(s)	2696
Asp700I	GAANNNNTTC	1 cut(s)	1749
AspA2I	CCTAGG	1 cut(s)	845
AspS9I	GGNCC	5 cut(s)	79, 879, 1501, 2192, 2704
AsuHPI	GGTGA	9 cut(s)	108, 155, 839, 848, 914, 1547, 1553, 1705, 2039
AvaI	CYCGRG	1 cut(s)	2102
AvaII	GGWCC	3 cut(s)	879, 1501, 2704
AvrII	CCTAGG	1 cut(s)	845
BaeGI	GKGCMC	1 cut(s)	818
BalI	TGGCCA	2 cut(s)	414, 2760
BanI	GGYRCC	2 cut(s)	815, 856
BanII	GRGCYC	1 cut(s)	732
BbrPI	CACGTG	1 cut(s)	1064
BbsI	GAAGAC	3 cut(s)	1313, 1752, 2072
Bbv12I	GWGCWC	2 cut(s)	732, 765
BbvI	GCAGC	3 cut(s)	1063, 1095, 2521
BccI	CCATC	6 cut(s)	134, 404, 827, 992, 1154, 1880
BceAI	ACGGC	1 cut(s)	1229
BcgI	CGANNNNNNTGC	2 cut(s)	1606, 1640
BciVI	GTATCC	1 cut(s)	882
BclI	TGATCA	4 cut(s)	703, 1429, 2218, 2521
BcoDI	GTCTC	5 cut(s)	140, 177, 1506, 2006, 2688
BfaI	CTAG	7 cut(s)	204, 645, 846, 993, 1020, 1343, 1667
BfmI	CTRYAG	4 cut(s)	1109, 1996, 2344, 2671
BfuI	GTATCC	1 cut(s)	882
BisI	GCNGC	3 cut(s)	1077, 1109, 2535
BlnI	CCTAGG	1 cut(s)	845
BlpI	GCTNAGC	1 cut(s)	1844
BlsI	GCNGC	3 cut(s)	1078, 1110, 2536
Bme18I	GGWCC	3 cut(s)	879, 1501, 2704
BmeRI	GACNNNNNGTC	1 cut(s)	1648
BmeT110I	CYCGRG	1 cut(s)	2102
BmgBI	CACGTC	1 cut(s)	1319
BmgT120I	GGNCC	5 cut(s)	79, 879, 1501, 2192, 2704
BmiI	GGNNCC	8 cut(s)	264, 430, 817, 858, 880, 1286, 1365, 1614
BmrI	ACTGGG	1 cut(s)	101
BmsI	GCATC	7 cut(s)	52, 313, 366, 490, 1057, 1706, 2680
BmuI	ACTGGG	1 cut(s)	101
BpiI	GAAGAC	3 cut(s)	1313, 1752, 2072
BpmI	CTGGAG	6 cut(s)	171, 977, 1359, 1530, 1676, 2787
Bpu10I	CCTNAGC	1 cut(s)	476
Bpu1102I	GCTNAGC	1 cut(s)	1844
BpuEI	CTTGAG	2 cut(s)	1212, 1264
BsaAI	YACGTR	1 cut(s)	1064
BsaBI	GATNNNNATC	2 cut(s)	837, 1252
BsaJI	CCNNGG	8 cut(s)	415, 845, 875, 1029, 1392, 1590, 1616, 2827
BsaXI	ACNNNNNCTCC	6 cut(s)	886, 916, 1503, 1533, 2311, 2341
Bsc4I	CCNNNNNNNGG	8 cut(s)	242, 309, 636, 717, 718, 1030, 1272, 2042
Bse1I	ACTGG	5 cut(s)	107, 154, 1723, 1988, 2849
Bse3DI	GCAATG	2 cut(s)	624, 2556
Bse8I	GATNNNNATC	2 cut(s)	837, 1252
BseDI	CCNNGG	8 cut(s)	415, 845, 875, 1029, 1392, 1590, 1616, 2827
BseJI	GATNNNNATC	2 cut(s)	837, 1252
BseLI	CCNNNNNNNGG	8 cut(s)	242, 309, 636, 717, 718, 1030, 1272, 2042
BseMI	GCAATG	2 cut(s)	624, 2556
BseMII	CTCAG	8 cut(s)	90, 906, 1475, 1858, 1860, 1896, 2051, 2225
BseNI	ACTGG	5 cut(s)	107, 154, 1723, 1988, 2849
BseRI	GAGGAG	1 cut(s)	689
BseSI	GKGCMC	1 cut(s)	818
BseXI	GCAGC	3 cut(s)	1063, 1095, 2521
BseYI	CCCAGC	1 cut(s)	2836
BsgI	GTGCAG	1 cut(s)	824
Bsh1236I	CGCG	1 cut(s)	519
BshFI	GGCC	9 cut(s)	80, 338, 414, 1216, 1917, 2193, 2573, 2760, 2826
BshNI	GGYRCC	2 cut(s)	815, 856
BsiHKAI	GWGCWC	2 cut(s)	732, 765
BsiHKCI	CYCGRG	1 cut(s)	2102
BslFI	GGGAC	5 cut(s)	286, 388, 595, 892, 2727
BslI	CCNNNNNNNGG	8 cut(s)	242, 309, 636, 717, 718, 1030, 1272, 2042
BsmAI	GTCTC	5 cut(s)	140, 177, 1506, 2006, 2688
BsmBI	CGTCTC	1 cut(s)	177
BsmFI	GGGAC	5 cut(s)	286, 388, 595, 892, 2727
BsmI	GAATGC	1 cut(s)	91
BsnI	GGCC	9 cut(s)	80, 338, 414, 1216, 1917, 2193, 2573, 2760, 2826
BsoBI	CYCGRG	1 cut(s)	2102
Bsp1286I	GDGCHC	3 cut(s)	732, 765, 818
Bsp1407I	TGTACA	2 cut(s)	1929, 2618
Bsp1720I	GCTNAGC	1 cut(s)	1844
Bsp19I	CCATGG	2 cut(s)	415, 2827
Bsp68I	TCGCGA	1 cut(s)	519
BspACI	CCGC	6 cut(s)	659, 686, 842, 882, 1370, 2043
BspANI	GGCC	9 cut(s)	80, 338, 414, 1216, 1917, 2193, 2573, 2760, 2826
BspCNI	CTCAG	8 cut(s)	91, 907, 1474, 1857, 1861, 1895, 2050, 2224
BspFNI	CGCG	1 cut(s)	519
BspLI	GGNNCC	8 cut(s)	264, 430, 817, 858, 880, 1286, 1365, 1614
BspMAI	CTGCAG	3 cut(s)	1113, 2000, 2348
BspT107I	GGYRCC	2 cut(s)	815, 856
BsrBI	CCGCTC	1 cut(s)	2043
BsrDI	GCAATG	2 cut(s)	624, 2556
BsrGI	TGTACA	2 cut(s)	1929, 2618
BsrI	ACTGG	5 cut(s)	107, 154, 1723, 1988, 2849
BssECI	CCNNGG	8 cut(s)	415, 845, 875, 1029, 1392, 1590, 1616, 2827
BssT1I	CCWWGG	3 cut(s)	415, 845, 2827
Bst4CI	ACNGT	8 cut(s)	228, 256, 598, 1030, 1141, 1650, 1963, 2604
Bst6I	CTCTTC	4 cut(s)	130, 1395, 1445, 1735
BstAPI	GCANNNNNTGC	1 cut(s)	1085
BstAUI	TGTACA	2 cut(s)	1929, 2618
BstBAI	YACGTR	1 cut(s)	1064
BstC8I	GCNNGC	4 cut(s)	1419, 1487, 2304, 2588
BstDSI	CCRYGG	3 cut(s)	415, 1029, 2827
BstFNI	CGCG	1 cut(s)	519
BstMAI	GTCTC	5 cut(s)	140, 177, 1506, 2006, 2688
BstMWI	GCNNNNNNNGC	8 cut(s)	86, 509, 530, 656, 856, 1076, 1085, 2085
BstNSI	RCATGY	4 cut(s)	1157, 1489, 2534, 2556
BstSFI	CTRYAG	4 cut(s)	1109, 1996, 2344, 2671
BstSLI	GKGCMC	1 cut(s)	818
BstUI	CGCG	1 cut(s)	519
BstV1I	GCAGC	3 cut(s)	1063, 1095, 2521
BstV2I	GAAGAC	3 cut(s)	1313, 1752, 2072
BstX2I	RGATCY	7 cut(s)	102, 691, 1057, 1813, 2374, 2461, 2647
BstXI	CCANNNNNNTGG	1 cut(s)	2844
BstYI	RGATCY	7 cut(s)	102, 691, 1057, 1813, 2374, 2461, 2647
BsuI	GTATCC	1 cut(s)	882
BsuRI	GGCC	9 cut(s)	80, 338, 414, 1216, 1917, 2193, 2573, 2760, 2826
BtgI	CCRYGG	3 cut(s)	415, 1029, 2827
BtrI	CACGTC	1 cut(s)	1319
BtsI	GCAGTG	3 cut(s)	812, 1570, 2353
BtsIMutI	CAGTG	8 cut(s)	96, 812, 1146, 1469, 1570, 1716, 1815, 2353
BtuMI	TCGCGA	1 cut(s)	519
Cac8I	GCNNGC	4 cut(s)	1419, 1487, 2304, 2588
CaiI	CAGNNNCTG	1 cut(s)	2135
Cfr13I	GGNCC	5 cut(s)	79, 879, 1501, 2192, 2704
CseI	GACGC	2 cut(s)	194, 1976
Csp6I	GTAC	5 cut(s)	289, 1125, 1193, 1930, 2619
CspCI	CAANNNNNGTGG	2 cut(s)	2500, 2535
CviQI	GTAC	5 cut(s)	289, 1125, 1193, 1930, 2619
DriI	GACNNNNNGTC	1 cut(s)	1648
EaeI	YGGCCR	3 cut(s)	412, 1214, 2758
Eam1104I	CTCTTC	4 cut(s)	130, 1395, 1445, 1735
Eam1105I	GACNNNNNGTC	1 cut(s)	1648
EarI	CTCTTC	4 cut(s)	130, 1395, 1445, 1735
EciI	GGCGGA	2 cut(s)	831, 1359
Ecl136II	GAGCTC	1 cut(s)	730
Eco130I	CCWWGG	3 cut(s)	415, 845, 2827
Eco147I	AGGCCT	1 cut(s)	1917
Eco24I	GRGCYC	1 cut(s)	732
Eco47I	GGWCC	3 cut(s)	879, 1501, 2704
Eco53kI	GAGCTC	1 cut(s)	730
Eco57I	CTGAAG	4 cut(s)	809, 1426, 2469, 2719
Eco72I	CACGTG	1 cut(s)	1064
Eco88I	CYCGRG	1 cut(s)	2102
EcoICRI	GAGCTC	1 cut(s)	730
EcoRI	GAATTC	2 cut(s)	538, 1758
EcoT14I	CCWWGG	3 cut(s)	415, 845, 2827
EcoT22I	ATGCAT	2 cut(s)	2558, 2695
EcoT38I	GRGCYC	1 cut(s)	732
ErhI	CCWWGG	3 cut(s)	415, 845, 2827
Esp3I	CGTCTC	1 cut(s)	177
FalI	AAGNNNNNCTT	2 cut(s)	2286, 2318
FaqI	GGGAC	5 cut(s)	286, 388, 595, 892, 2727
FbaI	TGATCA	4 cut(s)	703, 1429, 2218, 2521
FblI	GTMKAC	1 cut(s)	2730
Fnu4HI	GCNGC	3 cut(s)	1077, 1109, 2535
FriOI	GRGCYC	1 cut(s)	732
Fsp4HI	GCNGC	3 cut(s)	1077, 1109, 2535
FspBI	CTAG	7 cut(s)	204, 645, 846, 993, 1020, 1343, 1667
GluI	GCNGC	3 cut(s)	1077, 1109, 2535
GsaI	CCCAGC	1 cut(s)	2840
GsuI	CTGGAG	6 cut(s)	171, 977, 1359, 1530, 1676, 2787
HaeIII	GGCC	9 cut(s)	80, 338, 414, 1216, 1917, 2193, 2573, 2760, 2826
HgaI	GACGC	2 cut(s)	194, 1976
HincII	GTYRAC	2 cut(s)	1830, 1966
HindII	GTYRAC	2 cut(s)	1830, 1966
HindIII	AAGCTT	1 cut(s)	2204
HphI	GGTGA	9 cut(s)	108, 155, 839, 848, 914, 1547, 1553, 1705, 2039
Hpy166II	GTNNAC	3 cut(s)	1830, 1966, 2731
Hpy8I	GTNNAC	3 cut(s)	1830, 1966, 2731
HpyCH4III	ACNGT	8 cut(s)	228, 256, 598, 1030, 1141, 1650, 1963, 2604
HpyCH4IV	ACGT	3 cut(s)	1063, 1318, 2664
HpyF10VI	GCNNNNNNNGC	8 cut(s)	86, 509, 530, 656, 856, 1076, 1085, 2085
HpySE526I	ACGT	3 cut(s)	1063, 1318, 2664
Ksp22I	TGATCA	4 cut(s)	703, 1429, 2218, 2521
LmnI	GCTCC	2 cut(s)	958, 2806
Lsp1109I	GCAGC	3 cut(s)	1063, 1095, 2521
LweI	GCATC	7 cut(s)	52, 313, 366, 490, 1057, 1706, 2680
MaeI	CTAG	7 cut(s)	204, 645, 846, 993, 1020, 1343, 1667
MaeII	ACGT	3 cut(s)	1063, 1318, 2664
MaeIII	GTNAC	3 cut(s)	1240, 1541, 1711
MbiI	CCGCTC	1 cut(s)	2043
MfeI	CAATTG	3 cut(s)	921, 1080, 1896
MflI	RGATCY	7 cut(s)	102, 691, 1057, 1813, 2374, 2461, 2647
MhlI	GDGCHC	3 cut(s)	732, 765, 818
MlsI	TGGCCA	2 cut(s)	414, 2760
MluNI	TGGCCA	2 cut(s)	414, 2760
MlyI	GAGTC	2 cut(s)	926, 1451
MmeI	TCCRAC	1 cut(s)	2682
Mox20I	TGGCCA	2 cut(s)	414, 2760
Mph1103I	ATGCAT	2 cut(s)	2558, 2695
MroXI	GAANNNNTTC	1 cut(s)	1749
MscI	TGGCCA	2 cut(s)	414, 2760
MseI	TTAA	8 cut(s)	741, 1736, 2124, 2252, 2265, 2369, 2450, 2696
MslI	CAYNNNNRTG	1 cut(s)	1158
Msp20I	TGGCCA	2 cut(s)	414, 2760
MspA1I	CMGCKG	2 cut(s)	1640, 2840
MunI	CAATTG	3 cut(s)	921, 1080, 1896
Mva1269I	GAATGC	1 cut(s)	91
MvnI	CGCG	1 cut(s)	519
MwoI	GCNNNNNNNGC	8 cut(s)	86, 509, 530, 656, 856, 1076, 1085, 2085
NcoI	CCATGG	2 cut(s)	415, 2827
NlaIV	GGNNCC	8 cut(s)	264, 430, 817, 858, 880, 1286, 1365, 1614
NmuCI	GTSAC	2 cut(s)	1541, 1711
NruI	TCGCGA	1 cut(s)	519
NsiI	ATGCAT	2 cut(s)	2558, 2695
NspI	RCATGY	4 cut(s)	1157, 1489, 2534, 2556
PaeI	GCATGC	1 cut(s)	1489
PceI	AGGCCT	1 cut(s)	1917
PctI	GAATGC	1 cut(s)	91
PdmI	GAANNNNTTC	1 cut(s)	1749
PfeI	GAWTC	9 cut(s)	641, 793, 905, 970, 1091, 1253, 1859, 2180, 2258
PflMI	CCANNNNNTGG	2 cut(s)	1030, 1272
PkrI	GCNGC	3 cut(s)	1078, 1110, 2536
PleI	GAGTC	2 cut(s)	925, 1451
PmaCI	CACGTG	1 cut(s)	1064
PmlI	CACGTG	1 cut(s)	1064
PpsI	GAGTC	2 cut(s)	925, 1451
Ppu21I	YACGTR	1 cut(s)	1064
PshBI	ATTAAT	1 cut(s)	2696
PsiI	TTATAA	1 cut(s)	1098
Psp124BI	GAGCTC	1 cut(s)	732
Psp1406I	AACGTT	1 cut(s)	2664
PspCI	CACGTG	1 cut(s)	1064
PspFI	CCCAGC	1 cut(s)	2836
PspN4I	GGNNCC	8 cut(s)	264, 430, 817, 858, 880, 1286, 1365, 1614
PspPI	GGNCC	5 cut(s)	79, 879, 1501, 2192, 2704
PsrI	GAACNNNNNNTAC	2 cut(s)	37, 69
PstI	CTGCAG	3 cut(s)	1113, 2000, 2348
PstNI	CAGNNNCTG	1 cut(s)	2135
PsuI	RGATCY	7 cut(s)	102, 691, 1057, 1813, 2374, 2461, 2647
PvuII	CAGCTG	2 cut(s)	1640, 2840
RruI	TCGCGA	1 cut(s)	519
RsaI	GTAC	5 cut(s)	290, 1126, 1194, 1931, 2620
RsaNI	GTAC	5 cut(s)	289, 1125, 1193, 1930, 2619
RseI	CAYNNNNRTG	1 cut(s)	1158
SacI	GAGCTC	1 cut(s)	732
SaqAI	TTAA	8 cut(s)	741, 1736, 2124, 2252, 2265, 2369, 2450, 2696
SatI	GCNGC	3 cut(s)	1077, 1109, 2535
Sau96I	GGNCC	5 cut(s)	79, 879, 1501, 2192, 2704
SchI	GAGTC	2 cut(s)	926, 1451
SduI	GDGCHC	3 cut(s)	732, 765, 818
SfaNI	GCATC	7 cut(s)	52, 313, 366, 490, 1057, 1706, 2680
SfcI	CTRYAG	4 cut(s)	1109, 1996, 2344, 2671
SinI	GGWCC	3 cut(s)	879, 1501, 2704
SmiMI	CAYNNNNRTG	1 cut(s)	1158
SmlI	CTYRAG	2 cut(s)	1227, 1279
SmoI	CTYRAG	2 cut(s)	1227, 1279
SphI	GCATGC	1 cut(s)	1489
SseBI	AGGCCT	1 cut(s)	1917
SsiI	CCGC	6 cut(s)	659, 686, 842, 882, 1370, 2043
SspI	AATATT	1 cut(s)	1836
SspMI	CTAG	7 cut(s)	204, 645, 846, 993, 1020, 1343, 1667
SstI	GAGCTC	1 cut(s)	732
StuI	AGGCCT	1 cut(s)	1917
StyI	CCWWGG	3 cut(s)	415, 845, 2827
TaaI	ACNGT	8 cut(s)	228, 256, 598, 1030, 1141, 1650, 1963, 2604
TaiI	ACGT	3 cut(s)	1066, 1321, 2667
TatI	WGTACW	3 cut(s)	1192, 1929, 2618
TfiI	GAWTC	9 cut(s)	641, 793, 905, 970, 1091, 1253, 1859, 2180, 2258
Tru1I	TTAA	8 cut(s)	741, 1736, 2124, 2252, 2265, 2369, 2450, 2696
Tru9I	TTAA	8 cut(s)	741, 1736, 2124, 2252, 2265, 2369, 2450, 2696
TscAI	CASTG	8 cut(s)	103, 812, 1146, 1469, 1570, 1723, 1815, 2353
TseFI	GTSAC	2 cut(s)	1541, 1711
TseI	GCWGC	3 cut(s)	1076, 1108, 2534
Tsp45I	GTSAC	2 cut(s)	1541, 1711
TspDTI	ATGAA	4 cut(s)	1131, 1866, 1926, 2613
TspGWI	ACGGA	3 cut(s)	357, 1620, 1690
TspRI	CASTG	8 cut(s)	103, 812, 1146, 1469, 1570, 1723, 1815, 2353
Van91I	CCANNNNNTGG	2 cut(s)	1030, 1272
VpaK11BI	GGWCC	3 cut(s)	879, 1501, 2704
VspI	ATTAAT	1 cut(s)	2696
XapI	RAATTY	7 cut(s)	469, 538, 1336, 1571, 1758, 2174, 2383
XbaI	TCTAGA	2 cut(s)	644, 1342
XceI	RCATGY	4 cut(s)	1157, 1489, 2534, 2556
XcmI	CCANNNNNNNNNTGG	3 cut(s)	368, 422, 598
XmaJI	CCTAGG	1 cut(s)	845
XmiI	GTMKAC	1 cut(s)	2730
XmnI	GAANNNNTTC	1 cut(s)	1749
XspI	CTAG	7 cut(s)	204, 645, 846, 993, 1020, 1343, 1667
Zsp2I	ATGCAT	2 cut(s)	2558, 2695

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.