Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0006775

Physical Location & Seq

Genomic Coordinates Reverse (-)

87564299 .. 87572710

Gene Structure 8412 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00150130.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3246 bp

ATGGGTTGCGTGTACTCGAGGGTCTGTATTGGAGAGGTTAGTAGTAGTAGTAGTTCAAGAGATGCAAGAAGGAGAGAGGCCCAGAATGTGAGGAACACTGAGATCCCAGTTTTCTCACCCAACTCTTCAAGTGAGGAAGATGGTGAGATTGGAGATCAGTTCAACCAGGCAAACCATACCAGAGATGCTGAAATGGGGATCACTAGGCTGTCGAGGGTTTCGGCACAGTTTCTACCTCCAAATGGGTCGAGAACTGTGAAGGTTCCTTCTGGCAAGTTTGAGTTGAGGTACTCGTATTTGTCCCAGCGAGGGTATTACCCTGATGCTCTTGATAAGGCTAACCAGGACAGCTTTTGCATCCACACTCCGTTTGGGGCGAACCCGGATGACCATTTTTTCGGGGTGTTTGATGGCCATGGAGAGTTTGGAACCGAGTGTTCGCAGTTTGTCAAGAGGAAGCTGTGTGAGAATTTGCTCAGGCATGGTAAGTTTCAAGTGGATGCTGTCGAGGCGTGTCATGAGGCCTTTCTTGCCACGAATTCGCAGCTGCATGATGACATTGTGGATGATACCATGAGTGGAACAACAGCCATTACGGTTTTGGTTAGAGGTAGGAAGATATACATTGCCAATTCGGGGGATTCTAGGGCTGTTATAGCCGAGAGGAAAGGGGAGGAGTTGGTGGCCGTGGATCTTTCGATTGATCAGACCCCGTTTCGGGTGGATGAGCTCGAAAGGGTTAAGATTTGTGGTGCAAGAGTGCTTACATTGGATCAGATTGAAGGGCTGAAGAATCCAGACGTGCAGTGTTGGGGCACCGAAGAAGGTGATGATGGTGATCCGCCTAGGCTGTGGGTGCCCAATGGAATGTATCCTGGGACCGCTTTTACGAGGAGTATTGGTGATTCCATTGCTGAGTCAATTGGAGTTGTTGCCAACCCTGAAATTGTGGTTTTGGAGCTTACACAGAATCATCCTTTCTTCGTGCTTGCTAGTGATGGAGTGTTTGAGTTTATTTCTAGCCAAACCGTGGTTGATATGGTTGCAAAATATAAAGATCCACGTGATGCTTGTGCTGCAATAGTTGCCGAATCTTATAAACTCTGGCTGCAGTATGAAACCCGTACAGATGATATTACAGTGATCGTGGTGCATGTAGATGGGCTAACTGCTACTGCTGTTGGTCAATCAGTACATCCTGGTTCTGTTTTACGGCCACCTGTTCCTCAAGTTGTAGAGGTTACAGGATCAGAATCTCCTTCAACCATTAGCTGGAACGCAAGGAACCCTCGCATAAAACATGATTTGTCAAAGGCACGTCTTCGTGTGATTGAAAATTCTCTAGAAAATGGGCAAGTTTGGGTTCCTCCGCCTCCAGCCCACAGAAAAACCTGGGAGGAAGAAGCACAAATTGAGCGAGCTTTACATGATCATTTCCTCTTCAGAAAACTTACCGACTCTCAGTGTCATGTTTTATTGGATTGCATGCAAAGGGTTGAGGTCCAGCCTGGAGACATTGTTGTTAGACAGGGTGGTGAAGGTGACTGCTTTTATGTTGTTGGCAGTGGAGAATTTGAGGTGTCAGCAACCCAGGAAGAAAAGAATGGAGAGGTTCCAAGGGTTTTGCAACGATATACAGCTGACAAACTGTCATCCTTTGGAGAACTAGCACTAATGTATAACAAACCACTCCAGGCATCCGTTCGTGCTGTCACCACTGGAACTCTCTGGGCTTTAAGAAGAGAAGACTTTCGTGGAATTCTAATGTCAGAGTTTTCTAATTTGTCATATTTGAAGTTGCTTCGATCAGTGGATCTTTTATCCAGGCTGACAATATTACAGCTCAGCCATATTGCTGATTCTCTTTCTGAGGTTTCATTCTCAGATGGGCAAACAATTGTCAATGAGAATGAAGGCCTATTGGCATTGTACATTATCCAGAAGGGAAAAGTGAGGATTACAGTTGACGCAAGTTCAGTTAGCAATCCAGTTGTCTGCAATCTCATGTCTGACAATCAAAAACAAGGTGATAATCCTCAGAGCGGTAAAGAGATAATAGTGGAGAAGACAGAGGGAAGCTACTTTGGCGAATGGACACTTCTCGGGGAACATATTGATTTGTTTAATGCAGTTGCTGTGGGGGATGTTGTTTGTGCTGTTTTGACAAAGGAGAAATTTGATTCAGTCATTGGCCCAATGACAAAGCTTACTCAGGACGATCAGAAATCAAGAGATCAGTCTTCGGAAACTTTAATGGAATCTGTTAAAAGTATTGATATCTCTGCTCTTACTAAAGTTCAGCTTGCTGATATGGAGTGGAGAAAGTGTCTGTATTCTACTGACTGCAGTGAGATCGGGCTTGTTCTTTTAAGAGATCCAGAAAATTTGCTTAGTTTGAAAAGGTTTTCAAAGAAGAAGGTCAGAAAGCTTGGAAAGGAAGCACAAGTATTAAAAGAGAAGGATCTAATCAAGAGCATAAGCCCTTCAGCTTGTGTGCCACAGGTTCTATCCACTTGTGTTGATCAGACACATGCTGCAATACTGCTCAATACATGCATTGCTTGTCCATTGGCCTCGATACTTCGCACGCCCCTTGATGAACCATCTGCCCGATTTTGTACAGCCTCTCTAATTACTGCTTTGGAGGATCTCCACAAGAATGACGTTCTCTACAGAGGCTTGTCTCCTGATGCATTAATGTTGGATCACACGGGACATTTACAGCTTGTAGACTTCAGATTTGGGAAAAAGTTGTCTGGCCAAAGAACATATACAATTTGTGGAATGGCAGACTTTTTAGCTCCAGAGGTAGTTCAGGGAATAGGCCATGGTTTCCCAGCTGACTGGTGGGCATTGGGAGTCTTGATATATTTCATGTTACAAGGTGAATTGCCTTTTGGGTCATGGAGAGAAAGTGAGCTTGATATCTTTGCAAAGATTGCGAAGGGACAGCTTAATCTTCCTCAAACTTTCAGCCCTGGAGTTGTTGATCTCATTACCAAGTTACTTGAGGTTGATGAAAACACCAGACTAGGAAGCCAAGGTTGTGATTCTGTTAAAAGTCATCCCTGGTTCGATGGTATTGATTGGAAAGGGATTAGAGACTCTAGTTTTCCTGTTCCTCAGGAGATCACCTCTCGCATAGCTCAACATTTGGAAAGTCACTCAGACGAGTATAGTGCTCCTCAAGAGGAGGAGGACGGAGGTATTTGCTTTTTGACGGTGTTTCCGTGGCTCGAGGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko04022 ko04540 ko04611 ko04713 ko04714 ko04730 ko04740 ko04923 ko04924 ko04970 map04022 map04540 map04611 map04713 map04714 map04730 map04740 map04923 map04924 map04970

Pfam Domains

Protein Families

PP2C Pkinase cNMP_binding

Protein Analysis

1081

Amino Acids

119.84

Weight (kDa)

5.07

Isoelectric Point (pI)

39.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PP2C	PF00481	120 - 364	5.3e-34	Protein phosphatase 2C
cNMP_binding	PF00027	499 - 590	8.7e-16	Cyclic nucleotide-binding domain
Pkinase	PF00069	791 - 1025	3.2e-36	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	812 - 1011	3.4e-09	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006775)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G20050 AT2G20050
fragaria_vesca	FvH4_1g05740 FvH4_1g05740 FvH4_1g05740
malus_domestica	MD02G1058500.v1.1 MD15G1192800.v1.1
prunus_persica	Prupe.7G224500_v2.0.a1 Prupe.7G224500_v2.0.a1 Prupe.7G224500_v2.0.a1 Prupe.7G224500_v2.0.a1
pyrus_communis	pycom02g04740 pycom15g17250 pycom15g17260
rosa_chinensis	RchiOBHm_Chr2g0091381
rosa_laevigata	RLG00000016219
rosa_multiflora	Rmu_ssc0000333.1_g000004 Rmu_ssc0000333.1_g000011
rosa_roxburghii	Rroxscaffold_2G00150130
rosa_rugosa	Rorug02G0017200 Rorug02G0017300
rosa_samantha	Rh2AG062300 Rh2BG061300 Rh2CG063400 Rh2DG061900
rosa_wichuraiana	Rw2G005790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1098
AccB1I	GGYRCC	2 cut(s)	815, 856
AccB7I	CCANNNNNTGG	2 cut(s)	1030, 1272
AccBSI	CCGCTC	1 cut(s)	2043
AccI	GTMKAC	1 cut(s)	2730
AciI	CCGC	4 cut(s)	842, 882, 1370, 2043
AcoI	YGGCCR	4 cut(s)	412, 684, 1214, 2758
AcsI	RAATTY	7 cut(s)	469, 538, 1336, 1571, 1758, 2174, 2383
AcuI	CTGAAG	4 cut(s)	809, 1426, 2469, 2719
AcvI	CACGTG	1 cut(s)	1064
AfaI	GTAC	6 cut(s)	14, 290, 1126, 1194, 1931, 2620
AfiI	CCNNNNNNNGG	8 cut(s)	242, 309, 636, 717, 718, 1030, 1272, 2042
AhdI	GACNNNNNGTC	1 cut(s)	1648
AjiI	CACGTC	2 cut(s)	802, 1319
AjuI	GAANNNNNNNTTGG	2 cut(s)	2880, 2912
Alw21I	GWGCWC	2 cut(s)	732, 3184
Alw26I	GTCTC	3 cut(s)	1506, 2688, 3096
AlwNI	CAGNNNCTG	1 cut(s)	2135
Ama87I	CYCGRG	3 cut(s)	16, 2102, 3236
ApeKI	GCWGC	5 cut(s)	544, 547, 1076, 1108, 2534
ApoI	RAATTY	7 cut(s)	469, 538, 1336, 1571, 1758, 2174, 2383
ArsI	GACNNNNNNTTYG	4 cut(s)	380, 412, 2723, 2755
AseI	ATTAAT	1 cut(s)	2696
Asp700I	GAANNNNTTC	1 cut(s)	1749
AspA2I	CCTAGG	1 cut(s)	845
AspS9I	GGNCC	4 cut(s)	79, 879, 1501, 2192
AsuC2I	CCSGG	1 cut(s)	383
AvaI	CYCGRG	3 cut(s)	16, 2102, 3236
AvaII	GGWCC	2 cut(s)	879, 1501
AvrII	CCTAGG	1 cut(s)	845
AxyI	CCTNAGG	1 cut(s)	3123
BaeGI	GKGCMC	2 cut(s)	818, 861
BalI	TGGCCA	2 cut(s)	414, 2760
BanI	GGYRCC	2 cut(s)	815, 856
BanII	GRGCYC	1 cut(s)	732
BbrPI	CACGTG	1 cut(s)	1064
BbsI	GAAGAC	4 cut(s)	1313, 1752, 2072, 2232
Bbv12I	GWGCWC	2 cut(s)	732, 3184
BbvI	GCAGC	5 cut(s)	534, 556, 1063, 1095, 2521
BccI	CCATC	8 cut(s)	134, 404, 827, 992, 1154, 1880, 2611, 3071
BceAI	ACGGC	2 cut(s)	671, 1229
BcgI	CGANNNNNNTGC	4 cut(s)	2335, 2369, 3161, 3195
BciVI	GTATCC	1 cut(s)	882
BclI	TGATCA	3 cut(s)	703, 1429, 2521
BcnI	CCSGG	1 cut(s)	383
BcoDI	GTCTC	3 cut(s)	1506, 2688, 3096
BfaI	CTAG	9 cut(s)	204, 645, 846, 993, 1020, 1343, 1667, 3032, 3108
BfmI	CTRYAG	3 cut(s)	1109, 2344, 2671
BfuI	GTATCC	1 cut(s)	882
BisI	GCNGC	5 cut(s)	545, 548, 1077, 1109, 2535
BlnI	CCTAGG	1 cut(s)	845
BlpI	GCTNAGC	1 cut(s)	1844
BlsI	GCNGC	5 cut(s)	546, 549, 1078, 1110, 2536
Bme18I	GGWCC	2 cut(s)	879, 1501
BmeRI	GACNNNNNGTC	1 cut(s)	1648
BmeT110I	CYCGRG	3 cut(s)	16, 2102, 3236
BmgBI	CACGTC	2 cut(s)	802, 1319
BmgT120I	GGNCC	4 cut(s)	79, 879, 1501, 2192
BmiI	GGNNCC	8 cut(s)	264, 430, 817, 858, 880, 1286, 1365, 1614
BmrI	ACTGGG	1 cut(s)	101
BmsI	GCATC	8 cut(s)	52, 175, 313, 366, 490, 1057, 1706, 2680
BmuI	ACTGGG	1 cut(s)	101
BpiI	GAAGAC	4 cut(s)	1313, 1752, 2072, 2232
BplI	GAGNNNNNCTC	2 cut(s)	3119, 3151
BpmI	CTGGAG	5 cut(s)	1359, 1530, 1676, 2787, 3000
Bpu10I	CCTNAGC	1 cut(s)	476
Bpu1102I	GCTNAGC	1 cut(s)	1844
BpuEI	CTTGAG	3 cut(s)	1212, 3029, 3171
BpuMI	CCSGG	1 cut(s)	383
BsaAI	YACGTR	1 cut(s)	1064
BsaBI	GATNNNNATC	2 cut(s)	837, 1252
BsaXI	ACNNNNNCTCC	8 cut(s)	886, 916, 1503, 1533, 2311, 2341, 2899, 2929
Bsc4I	CCNNNNNNNGG	8 cut(s)	242, 309, 636, 717, 718, 1030, 1272, 2042
Bse1I	ACTGG	4 cut(s)	107, 1723, 1988, 2849
Bse21I	CCTNAGG	1 cut(s)	3123
Bse3DI	GCAATG	3 cut(s)	624, 909, 2556
Bse8I	GATNNNNATC	2 cut(s)	837, 1252
BseJI	GATNNNNATC	2 cut(s)	837, 1252
BseLI	CCNNNNNNNGG	8 cut(s)	242, 309, 636, 717, 718, 1030, 1272, 2042
BseMI	GCAATG	3 cut(s)	624, 909, 2556
BseNI	ACTGG	4 cut(s)	107, 1723, 1988, 2849
BseRI	GAGGAG	5 cut(s)	689, 907, 3174, 3206, 3209
BseSI	GKGCMC	2 cut(s)	818, 861
BseXI	GCAGC	5 cut(s)	534, 556, 1063, 1095, 2521
BseYI	CCCAGC	2 cut(s)	303, 2836
BsgI	GTGCAG	1 cut(s)	824
BshNI	GGYRCC	2 cut(s)	815, 856
BsiHKAI	GWGCWC	2 cut(s)	732, 3184
BsiHKCI	CYCGRG	3 cut(s)	16, 2102, 3236
BsiSI	CCGG	1 cut(s)	383
BslFI	GGGAC	4 cut(s)	286, 892, 2727, 2961
BslI	CCNNNNNNNGG	8 cut(s)	242, 309, 636, 717, 718, 1030, 1272, 2042
BsmAI	GTCTC	3 cut(s)	1506, 2688, 3096
BsmFI	GGGAC	4 cut(s)	286, 892, 2727, 2961
BsoBI	CYCGRG	3 cut(s)	16, 2102, 3236
Bsp1286I	GDGCHC	4 cut(s)	732, 818, 861, 3184
Bsp1407I	TGTACA	2 cut(s)	1929, 2618
Bsp1720I	GCTNAGC	1 cut(s)	1844
Bsp19I	CCATGG	2 cut(s)	415, 2827
BspACI	CCGC	4 cut(s)	842, 882, 1370, 2043
BspHI	TCATGA	1 cut(s)	517
BspLI	GGNNCC	8 cut(s)	264, 430, 817, 858, 880, 1286, 1365, 1614
BspMAI	CTGCAG	2 cut(s)	1113, 2348
BspT107I	GGYRCC	2 cut(s)	815, 856
BsrBI	CCGCTC	1 cut(s)	2043
BsrDI	GCAATG	3 cut(s)	624, 909, 2556
BsrGI	TGTACA	2 cut(s)	1929, 2618
BsrI	ACTGG	4 cut(s)	107, 1723, 1988, 2849
BssT1I	CCWWGG	5 cut(s)	415, 845, 1616, 2827, 3040
Bst4CI	ACNGT	8 cut(s)	228, 256, 598, 1030, 1141, 1650, 1963, 3223
Bst6I	CTCTTC	3 cut(s)	130, 1445, 1735
BstAPI	GCANNNNNTGC	2 cut(s)	1085, 2939
BstAUI	TGTACA	2 cut(s)	1929, 2618
BstBAI	YACGTR	1 cut(s)	1064
BstC8I	GCNNGC	5 cut(s)	990, 1419, 1487, 2304, 2588
BstDSI	CCRYGG	5 cut(s)	415, 687, 1029, 2827, 3230
BstMAI	GTCTC	3 cut(s)	1506, 2688, 3096
BstMWI	GCNNNNNNNGC	8 cut(s)	509, 530, 656, 856, 1076, 1085, 2085, 2939
BstNSI	RCATGY	4 cut(s)	1157, 1489, 2534, 2556
BstSFI	CTRYAG	3 cut(s)	1109, 2344, 2671
BstSLI	GKGCMC	2 cut(s)	818, 861
BstV1I	GCAGC	5 cut(s)	534, 556, 1063, 1095, 2521
BstV2I	GAAGAC	4 cut(s)	1313, 1752, 2072, 2232
BstX2I	RGATCY	7 cut(s)	102, 691, 1057, 1813, 2374, 2461, 2647
BstXI	CCANNNNNNTGG	1 cut(s)	2844
BstYI	RGATCY	7 cut(s)	102, 691, 1057, 1813, 2374, 2461, 2647
Bsu36I	CCTNAGG	1 cut(s)	3123
BsuI	GTATCC	1 cut(s)	882
BtgI	CCRYGG	5 cut(s)	415, 687, 1029, 2827, 3230
BtrI	CACGTC	2 cut(s)	802, 1319
BtsI	GCAGTG	3 cut(s)	812, 1570, 2353
BtsIMutI	CAGTG	8 cut(s)	96, 812, 1146, 1469, 1570, 1716, 1815, 2353
Cac8I	GCNNGC	5 cut(s)	990, 1419, 1487, 2304, 2588
CaiI	CAGNNNCTG	1 cut(s)	2135
CciI	TCATGA	1 cut(s)	517
Cfr13I	GGNCC	4 cut(s)	79, 879, 1501, 2192
CseI	GACGC	1 cut(s)	1976
Csp6I	GTAC	6 cut(s)	13, 289, 1125, 1193, 1930, 2619
CspCI	CAANNNNNGTGG	2 cut(s)	2500, 2535
CviQI	GTAC	6 cut(s)	13, 289, 1125, 1193, 1930, 2619
DriI	GACNNNNNGTC	1 cut(s)	1648
EaeI	YGGCCR	4 cut(s)	412, 684, 1214, 2758
Eam1104I	CTCTTC	3 cut(s)	130, 1445, 1735
Eam1105I	GACNNNNNGTC	1 cut(s)	1648
EarI	CTCTTC	3 cut(s)	130, 1445, 1735
EciI	GGCGGA	2 cut(s)	831, 1359
Ecl136II	GAGCTC	1 cut(s)	730
Eco130I	CCWWGG	5 cut(s)	415, 845, 1616, 2827, 3040
Eco147I	AGGCCT	2 cut(s)	524, 1917
Eco24I	GRGCYC	1 cut(s)	732
Eco32I	GATATC	2 cut(s)	2278, 2926
Eco47I	GGWCC	2 cut(s)	879, 1501
Eco53kI	GAGCTC	1 cut(s)	730
Eco57I	CTGAAG	4 cut(s)	809, 1426, 2469, 2719
Eco72I	CACGTG	1 cut(s)	1064
Eco81I	CCTNAGG	1 cut(s)	3123
Eco88I	CYCGRG	3 cut(s)	16, 2102, 3236
EcoICRI	GAGCTC	1 cut(s)	730
EcoRI	GAATTC	2 cut(s)	538, 1758
EcoRV	GATATC	2 cut(s)	2278, 2926
EcoT14I	CCWWGG	5 cut(s)	415, 845, 1616, 2827, 3040
EcoT22I	ATGCAT	2 cut(s)	2558, 2695
EcoT38I	GRGCYC	1 cut(s)	732
ErhI	CCWWGG	5 cut(s)	415, 845, 1616, 2827, 3040
FalI	AAGNNNNNCTT	2 cut(s)	2286, 2318
FaqI	GGGAC	4 cut(s)	286, 892, 2727, 2961
FbaI	TGATCA	3 cut(s)	703, 1429, 2521
FblI	GTMKAC	1 cut(s)	2730
Fnu4HI	GCNGC	5 cut(s)	545, 548, 1077, 1109, 2535
FriOI	GRGCYC	1 cut(s)	732
Fsp4HI	GCNGC	5 cut(s)	545, 548, 1077, 1109, 2535
FspBI	CTAG	9 cut(s)	204, 645, 846, 993, 1020, 1343, 1667, 3032, 3108
GluI	GCNGC	5 cut(s)	545, 548, 1077, 1109, 2535
GsaI	CCCAGC	2 cut(s)	307, 2840
GsuI	CTGGAG	5 cut(s)	1359, 1530, 1676, 2787, 3000
HapII	CCGG	1 cut(s)	383
HgaI	GACGC	1 cut(s)	1976
HincII	GTYRAC	1 cut(s)	1966
HindII	GTYRAC	1 cut(s)	1966
HindIII	AAGCTT	2 cut(s)	2204, 2426
HpaII	CCGG	1 cut(s)	383
Hpy166II	GTNNAC	3 cut(s)	13, 1966, 2731
Hpy8I	GTNNAC	3 cut(s)	13, 1966, 2731
HpyCH4III	ACNGT	8 cut(s)	228, 256, 598, 1030, 1141, 1650, 1963, 3223
HpyCH4IV	ACGT	4 cut(s)	801, 1063, 1318, 2664
HpyF10VI	GCNNNNNNNGC	8 cut(s)	509, 530, 656, 856, 1076, 1085, 2085, 2939
HpySE526I	ACGT	4 cut(s)	801, 1063, 1318, 2664
Ksp22I	TGATCA	3 cut(s)	703, 1429, 2521
LmnI	GCTCC	3 cut(s)	958, 2806, 3187
Lsp1109I	GCAGC	5 cut(s)	534, 556, 1063, 1095, 2521
LweI	GCATC	8 cut(s)	52, 175, 313, 366, 490, 1057, 1706, 2680
MaeI	CTAG	9 cut(s)	204, 645, 846, 993, 1020, 1343, 1667, 3032, 3108
MaeII	ACGT	4 cut(s)	801, 1063, 1318, 2664
MaeIII	GTNAC	6 cut(s)	1240, 1541, 1711, 2877, 3003, 3161
MbiI	CCGCTC	1 cut(s)	2043
MfeI	CAATTG	2 cut(s)	921, 1896
MflI	RGATCY	7 cut(s)	102, 691, 1057, 1813, 2374, 2461, 2647
MhlI	GDGCHC	4 cut(s)	732, 818, 861, 3184
MlsI	TGGCCA	2 cut(s)	414, 2760
MluNI	TGGCCA	2 cut(s)	414, 2760
MlyI	GAGTC	4 cut(s)	926, 1451, 2868, 3098
MmeI	TCCRAC	1 cut(s)	2682
Mox20I	TGGCCA	2 cut(s)	414, 2760
Mph1103I	ATGCAT	2 cut(s)	2558, 2695
MroXI	GAANNNNTTC	1 cut(s)	1749
MscI	TGGCCA	2 cut(s)	414, 2760
MslI	CAYNNNNRTG	2 cut(s)	1158, 2658
Msp20I	TGGCCA	2 cut(s)	414, 2760
MspA1I	CMGCKG	3 cut(s)	547, 1640, 2840
MspI	CCGG	1 cut(s)	383
MunI	CAATTG	2 cut(s)	921, 1896
MwoI	GCNNNNNNNGC	8 cut(s)	509, 530, 656, 856, 1076, 1085, 2085, 2939
NciI	CCSGG	1 cut(s)	383
NcoI	CCATGG	2 cut(s)	415, 2827
NlaIV	GGNNCC	8 cut(s)	264, 430, 817, 858, 880, 1286, 1365, 1614
NmeAIII	GCCGAG	1 cut(s)	685
NmuCI	GTSAC	3 cut(s)	1541, 1711, 3161
NsiI	ATGCAT	2 cut(s)	2558, 2695
NspI	RCATGY	4 cut(s)	1157, 1489, 2534, 2556
PaeI	GCATGC	1 cut(s)	1489
PaeR7I	CTCGAG	2 cut(s)	16, 3236
PagI	TCATGA	1 cut(s)	517
PceI	AGGCCT	2 cut(s)	524, 1917
PcsI	WCGNNNNNNNCGW	1 cut(s)	3227
PdmI	GAANNNNTTC	1 cut(s)	1749
PflMI	CCANNNNNTGG	2 cut(s)	1030, 1272
PkrI	GCNGC	5 cut(s)	546, 549, 1078, 1110, 2536
PleI	GAGTC	4 cut(s)	925, 1451, 2867, 3098
PmaCI	CACGTG	1 cut(s)	1064
PmlI	CACGTG	1 cut(s)	1064
PpsI	GAGTC	4 cut(s)	925, 1451, 2867, 3098
Ppu21I	YACGTR	1 cut(s)	1064
PshBI	ATTAAT	1 cut(s)	2696
PsiI	TTATAA	1 cut(s)	1098
Psp124BI	GAGCTC	1 cut(s)	732
PspCI	CACGTG	1 cut(s)	1064
PspFI	CCCAGC	2 cut(s)	303, 2836
PspN4I	GGNNCC	8 cut(s)	264, 430, 817, 858, 880, 1286, 1365, 1614
PspPI	GGNCC	4 cut(s)	79, 879, 1501, 2192
PspXI	VCTCGAGB	2 cut(s)	16, 3236
PsrI	GAACNNNNNNTAC	2 cut(s)	37, 69
PstI	CTGCAG	2 cut(s)	1113, 2348
PstNI	CAGNNNCTG	1 cut(s)	2135
PsuI	RGATCY	7 cut(s)	102, 691, 1057, 1813, 2374, 2461, 2647
PvuII	CAGCTG	3 cut(s)	547, 1640, 2840
RsaI	GTAC	6 cut(s)	14, 290, 1126, 1194, 1931, 2620
RsaNI	GTAC	6 cut(s)	13, 289, 1125, 1193, 1930, 2619
RseI	CAYNNNNRTG	2 cut(s)	1158, 2658
SacI	GAGCTC	1 cut(s)	732
SatI	GCNGC	5 cut(s)	545, 548, 1077, 1109, 2535
Sau96I	GGNCC	4 cut(s)	79, 879, 1501, 2192
SchI	GAGTC	4 cut(s)	926, 1451, 2868, 3098
SduI	GDGCHC	4 cut(s)	732, 818, 861, 3184
SfaNI	GCATC	8 cut(s)	52, 175, 313, 366, 490, 1057, 1706, 2680
SfcI	CTRYAG	3 cut(s)	1109, 2344, 2671
Sfr274I	CTCGAG	2 cut(s)	16, 3236
SinI	GGWCC	2 cut(s)	879, 1501
SlaI	CTCGAG	2 cut(s)	16, 3236
SmiMI	CAYNNNNRTG	2 cut(s)	1158, 2658
SmlI	CTYRAG	5 cut(s)	16, 1227, 3008, 3186, 3236
SmoI	CTYRAG	5 cut(s)	16, 1227, 3008, 3186, 3236
SphI	GCATGC	1 cut(s)	1489
SseBI	AGGCCT	2 cut(s)	524, 1917
SsiI	CCGC	4 cut(s)	842, 882, 1370, 2043
SspI	AATATT	1 cut(s)	1836
SspMI	CTAG	9 cut(s)	204, 645, 846, 993, 1020, 1343, 1667, 3032, 3108
SstI	GAGCTC	1 cut(s)	732
StuI	AGGCCT	2 cut(s)	524, 1917
StyI	CCWWGG	5 cut(s)	415, 845, 1616, 2827, 3040
TaaI	ACNGT	8 cut(s)	228, 256, 598, 1030, 1141, 1650, 1963, 3223
TaiI	ACGT	4 cut(s)	804, 1066, 1321, 2667
TatI	WGTACW	4 cut(s)	12, 1192, 1929, 2618
TscAI	CASTG	8 cut(s)	103, 812, 1146, 1469, 1570, 1723, 1815, 2353
TseFI	GTSAC	3 cut(s)	1541, 1711, 3161
TseI	GCWGC	5 cut(s)	544, 547, 1076, 1108, 2534
Tsp45I	GTSAC	3 cut(s)	1541, 1711, 3161
TspDTI	ATGAA	6 cut(s)	1131, 1866, 1926, 2613, 2863, 3033
TspGWI	ACGGA	4 cut(s)	357, 1690, 3216, 3219
TspRI	CASTG	8 cut(s)	103, 812, 1146, 1469, 1570, 1723, 1815, 2353
Van91I	CCANNNNNTGG	2 cut(s)	1030, 1272
VpaK11BI	GGWCC	2 cut(s)	879, 1501
VspI	ATTAAT	1 cut(s)	2696
XapI	RAATTY	7 cut(s)	469, 538, 1336, 1571, 1758, 2174, 2383
XbaI	TCTAGA	1 cut(s)	1342
XceI	RCATGY	4 cut(s)	1157, 1489, 2534, 2556
XcmI	CCANNNNNNNNNTGG	4 cut(s)	368, 422, 598, 2844
XhoI	CTCGAG	2 cut(s)	16, 3236
XmaJI	CCTAGG	1 cut(s)	845
XmiI	GTMKAC	1 cut(s)	2730
XmnI	GAANNNNTTC	1 cut(s)	1749
XspI	CTAG	9 cut(s)	204, 645, 846, 993, 1020, 1343, 1667, 3032, 3108
Zsp2I	ATGCAT	2 cut(s)	2558, 2695

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.