Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr2

Orthogroup ID

OG0006775

Physical Location & Seq

Genomic Coordinates Forward (+)

3074862 .. 3080813

Gene Structure 5952 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom02g04740.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3255 bp

ATGATGGGTTGTGTGTATTCGAGAGTTTGCATTGGAGAGGCTTGTTCTCCAAGAGGTCCGAAATTGAAAGAGAATCAGGATGTGAGGAGCGCTGAGATCCCAGTGTTCTCTCCTACTTCCTCCAATGGAGAAGTAGGTGAGCTTCAAGACCAGTTCAACCAGTCTGGCTTAACTGGGGATGCTGAGGTGGGTATCACTAGGCTCCGTAGGGTTTCATCACAGTTTCTGCCTCCCAATGGGTCAAGAACTGTCAAGGTTCCTTCGGGAAACTTCGAAATGCGGTATTCTTATTTGTCTCAGAGAGGTTATTACCCTGATGCTCTTGATAAGGCTAACCAAGATAGCTTTTGCATCCACACGCCATTCGGGACAAATCCGGATGACCATTTCTTTGGGGTCTTTGACGGTCATGGAGAATTCGGAGATCAGTGTTCGCAGTTTGTGAAAAGAAAGTTGTGTGAGAATTTGCTTAGGAATAGTAAGTTTCAAGTGGATGCTGTCGAGGCATGCCATTCTGCGTTTATTGCAACCAATTCTCAGTTGCACGCTGATAGTTTGGATGATAGCATGAGCGGGACAACTGCAATTACAGTTTTGGTTCGAGGTAGGACAATATGCATTGCGAATTCGGGTGATTCAAGGGCTGTTATAGCAGAGAGGAAAGGGGATGACATCGTGGCTGTGGATCGATCAATTGATCAAACCCCATTTAGAGTGGACGAGCTCGAAAGGGTTAAGCTTTGTGGTGCAAGAGTTCTTACATTGGATCAGATTGAAGGGCTGAAGAATCCAGATGTGCAGTGTTGGGGAACTGAAGAAAGCGATGATGGTGATCCACCTAGATTGTGGGTGCCAAACGGAATGTATCCTGGAACAGCTTTTACGAGGAGTATTGGTGATTCGACTGCTGAAACAATTGGGGTTGTTGCCAACCCTGAAATCGTGGTTTTAGAGCTTACACAGAATAATCCTTTCTTTGTGCTTGCTAGTGATGGAGTTTTTGAGTTCCTTTCTAGCCAAGCTGTGGTGGATATGGTTGCAAAATTTAAAGATCCCCGTGATGCTTGTGCTGCAATTGTAGCTGAATCATATAAACTCTGGCTGCAGTATGAAACTCGTACAGATGATATTACAGTGATCGTTGTGCATGTGAATGGGCTGACTGATATGTCCATCGGTCAATCAGTAAGCTGTGCTGCTGCTTTACGACCAACTGTTCCTCAAGTTGTAGAGGTTAAAAGATGCGAATCTCCTTCACCCACTGGGTGGAACTCTAGCAACCAGCGCACCAGACATGATTTGTCAAGGGCACGCCTTCGTGTGATTGAGAGTTCTCTAGAAAATGGGCAAATTTGGGTTCCTCCACCTCCATCCCACAGAAAGACTTGGGAGGAAGAAGCACACATTCAGCGGGCTTTGCATGACCATTTCCTCTTCAGAAAGCTCACTGATTCTCAGTGTCAGGTGTTATTGGATTGCATGGAAAGGGTTGAGTTCCAGCCTGGAGATGTTGTTGTTAGACAGGGTGGTGAGGGTGACTGCTTTTATGTTGTTGGCAGTGGAGAATTTGAGGTGTTGGCGACCCAGGAAGAAAAAGATGGAGAGCTTCCGAGGGTTCTGCAGCACTATACAGCTGATAAGCTGTCATCCTTTGGAGAACTAGCGCTAATGCATAACAAACCACTGCAGGCCTCCGTTCATGCTGTGACCAGTGGAACTCTTTGGGCTTTGAAAAGAGAAGACTTTCGTGGAATTTTAACGTCAGAGTTTTCTAATTTGTCATATTTGAAGTTGCTTCGATCAGTGGATCTTCTATCGAGGCTGACAATCTTACAGCTAAGCCACATCGCTGATTCTCTGTCTGAAGTTTCCTTTTCAGAAGGCCAGACAATAGTCAGTAAGAGTGAAGACCAGAGTGAAGACCTAGTTGGATTATACATTATCCGGAAGGGAAAAGTCAGGATTACTTGTGATGCAAATTCAGCTAGTAGTTCAGTTGTCCGGAGTCTCAAGTCTGACTATCAAAAAGAAGATGATCATCCTCAGAGCAGTAAAGAGCTCTCAGTGGAGAAGACGGAGGGAAGCTGCTTTGGTGAATGGGCACTTCTTGGAGAACGTATTGATTTGTTTAGAGCAGTTGCTGTGGGAGATGTCACCTGTGCTGTTTTAACAAAGGAAAATTTTGATTCAGCTGTTGGCCCTTTGACAAAGATTTCTCAGGATGATCAGAAGTCGAGGGATTATTCTTCAGAAGTTCCAAAGGAATCTGCCAAGAATATTGATATGTCTGCTCTTTCTGACGTCCAGTTGTCTGATCTAGAATGGAGAACAATTTTGTATAGCACTGACTGCAGTGAAATTGGAGTTGTTCGTTTAAGAGATTCAGAAAAATTGTTCAGTTTGAAAAGGTTTTCAAAGCAAATGGTCAGAAGGATGGGAAAGGAAGCGCAAGTGTTAAAAGAGAAGGATCTAATCAAGAGTATGAGCTCTTCAGCGTGTGTGCCACAGTTTCTGTGCACGTGTGTTGATCAGACACATGCTGGCATACTGTTTAATACTTGCCTTGCTTGTCCGTTGGCTTCGATACTTCGCACTCCCCTTGATGAACCGTCTGCTAAATTTTTTGCAGCCTCTCTAGTTGCTGGGTTGGCAGATCTCCACAAGAATGACGTTCTCTACAGAGGGTTGTCTCCTGATGTTATAATGTTGGACCAAACAGGATATTTACAGCTAGTGGACTTCAAGTTTGGGAAGAAGTTATCCGGCGAAAGAACATATACAATTTGTGGAATGGTGGACTTTTTAGCTCCAGAGGTAGTCCAGGGAAAAGGCCATGGTTTTCCATCCGACTGGTGGGCATTAGGAGTGTTGATATACTTCATGCTACGGGGTGAAATGCCATTTGGCTCATGGAGACAAAGTGAGCTTGATACATTTGCAAAGATCGCAAGGGGACAGCTAACTCTACCGCAAACTTTTAGTCCTGAAGTTGTTGATCTCATCACTAAGTTACTCGAGGTTGATGAAAACACCAGACTAGGGAGCCAAGGTTATGAATCTGTCAAAAGGCATCCCTGGTTTGATGGCACTGACTGGGAAGGGATCAAAGACCGTAGTTTGCCCGTTCCTCATGAGATCACCTCTCGCATAACTCAACATTTGGGAAGTCACTCCGAGAACTGTTCTGTTCCTCCAGCTTCTCCATCTCGAAATGGAGAAGAACTCGACACTCCTGAATGGTTTGACGACTGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko04022 ko04540 ko04611 ko04713 ko04714 ko04730 ko04740 ko04923 ko04924 ko04970 map04022 map04540 map04611 map04713 map04714 map04730 map04740 map04923 map04924 map04970

Pfam Domains

Protein Families

PP2C Pkinase cNMP_binding

Protein Analysis

1085

Amino Acids

120.31

Weight (kDa)

5.11

Isoelectric Point (pI)

43.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PP2C	PF00481	120 - 362	1.4e-31	Protein phosphatase 2C
cNMP_binding	PF00027	497 - 587	2.6e-15	Cyclic nucleotide-binding domain
Pkinase	PF00069	791 - 1027	4.2e-38	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	814 - 1012	1.8e-13	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006775)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G20050 AT2G20050
fragaria_vesca	FvH4_1g05740 FvH4_1g05740 FvH4_1g05740
malus_domestica	MD02G1058500.v1.1 MD15G1192800.v1.1
prunus_persica	Prupe.7G224500_v2.0.a1 Prupe.7G224500_v2.0.a1 Prupe.7G224500_v2.0.a1 Prupe.7G224500_v2.0.a1
pyrus_communis	pycom02g04740 pycom15g17250 pycom15g17260
rosa_chinensis	RchiOBHm_Chr2g0091381
rosa_laevigata	RLG00000016219
rosa_multiflora	Rmu_ssc0000333.1_g000004 Rmu_ssc0000333.1_g000011
rosa_roxburghii	Rroxscaffold_2G00150130
rosa_rugosa	Rorug02G0017200 Rorug02G0017300
rosa_samantha	Rh2AG062300 Rh2BG061300 Rh2CG063400 Rh2DG061900
rosa_wichuraiana	Rw2G005790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2702
AasI	GACNNNNNNGTC	1 cut(s)	1168
AatII	GACGTC	1 cut(s)	2304
AccB1I	GGYRCC	1 cut(s)	850
AccB7I	CCANNNNNTGG	2 cut(s)	1024, 1266
AccBSI	CCGCTC	1 cut(s)	573
AccIII	TCCGGA	3 cut(s)	376, 1944, 2001
AciI	CCGC	4 cut(s)	280, 573, 1411, 2969
AclWI	GGATC	8 cut(s)	91, 693, 774, 827, 1046, 1815, 2475, 3110
AcuI	CTGAAG	7 cut(s)	803, 834, 1420, 1884, 2232, 2475, 3006
AcvI	CACGTG	1 cut(s)	2520
AcyI	GRCGYC	1 cut(s)	2301
AdeI	CACNNNGTG	1 cut(s)	1266
AfaI	GTAC	1 cut(s)	1120
AfeI	AGCGCT	2 cut(s)	91, 1665
AfiI	CCNNNNNNNGG	5 cut(s)	236, 1024, 1266, 2853, 3039
AflIII	ACRYGT	1 cut(s)	2519
AjnI	CCWGG	5 cut(s)	868, 1501, 1584, 2820, 3074
AjuI	GAANNNNNNNTTGG	4 cut(s)	1911, 1943, 2886, 2918
Alw21I	GWGCWC	4 cut(s)	726, 2061, 2489, 2519
Alw26I	GTCTC	4 cut(s)	300, 2012, 2694, 2908
Alw44I	GTGCAC	1 cut(s)	2515
AlwI	GGATC	8 cut(s)	91, 693, 774, 827, 1046, 1815, 2475, 3110
AlwNI	CAGNNNCTG	3 cut(s)	226, 2141, 2512
Ama87I	CYCGRG	1 cut(s)	3014
Aor13HI	TCCGGA	3 cut(s)	376, 1944, 2001
Aor51HI	AGCGCT	2 cut(s)	91, 1665
AoxI	GGCC	4 cut(s)	1689, 1882, 2197, 2830
ApaLI	GTGCAC	1 cut(s)	2515
ApeKI	GCWGC	7 cut(s)	1070, 1102, 1196, 1199, 1621, 2085, 2627
ArsI	GACNNNNNNTTYG	6 cut(s)	374, 406, 2729, 2761, 2965, 2997
Asp700I	GAANNNNTTC	1 cut(s)	1743
AspLEI	GCGC	4 cut(s)	92, 1287, 1666, 2449
AspS9I	GGNCC	3 cut(s)	56, 2198, 2710
AsuII	TTCGAA	1 cut(s)	273
AvaI	CYCGRG	1 cut(s)	3014
AvaII	GGWCC	2 cut(s)	56, 2710
BaeGI	GKGCMC	3 cut(s)	1312, 2104, 2519
BanI	GGYRCC	1 cut(s)	850
BanII	GRGCYC	3 cut(s)	726, 2061, 2489
BarI	GAAGNNNNNNTAC	4 cut(s)	126, 158, 2473, 2505
BbrPI	CACGTG	1 cut(s)	2520
BbsI	GAAGAC	4 cut(s)	1746, 1914, 1926, 2078
Bbv12I	GWGCWC	4 cut(s)	726, 2061, 2489, 2519
BbvCI	CCTCAGC	1 cut(s)	183
BbvI	GCAGC	7 cut(s)	1057, 1089, 1183, 1186, 1633, 2072, 2639
BccI	CCATC	9 cut(s)	821, 986, 1181, 1378, 1592, 2428, 2851, 3077, 3211
BcgI	CGANNNNNNTGC	2 cut(s)	1600, 1634
BciT130I	CCWGG	5 cut(s)	870, 1503, 1586, 2822, 3076
BciVI	GTATCC	1 cut(s)	876
BclI	TGATCA	4 cut(s)	697, 2035, 2224, 2527
BcoDI	GTCTC	4 cut(s)	300, 2012, 2694, 2908
BfmI	CTRYAG	5 cut(s)	1103, 1619, 1685, 2350, 2677
BfoI	RGCGCY	2 cut(s)	93, 1667
BfuI	GTATCC	1 cut(s)	876
BglII	AGATCT	1 cut(s)	2653
BisI	GCNGC	7 cut(s)	1071, 1103, 1197, 1200, 1622, 2086, 2628
BlpI	GCTNAGC	1 cut(s)	1838
BlsI	GCNGC	7 cut(s)	1072, 1104, 1198, 1201, 1623, 2087, 2629
Bme1390I	CCNGG	5 cut(s)	870, 1503, 1586, 2822, 3076
Bme18I	GGWCC	2 cut(s)	56, 2710
BmeT110I	CYCGRG	1 cut(s)	3014
BmgT120I	GGNCC	3 cut(s)	56, 2198, 2710
BmiI	GGNNCC	5 cut(s)	203, 258, 852, 1359, 3044
BmrFI	CCNGG	5 cut(s)	870, 1503, 1586, 2822, 3076
BmrI	ACTGGG	4 cut(s)	95, 183, 1272, 3103
BmsI	GCATC	8 cut(s)	169, 307, 360, 484, 1051, 1232, 1963, 3079
BmuI	ACTGGG	4 cut(s)	95, 183, 1272, 3103
BoxI	GACNNNNGTC	1 cut(s)	1892
BpiI	GAAGAC	4 cut(s)	1746, 1914, 1926, 2078
BplI	GAGNNNNNCTC	4 cut(s)	3125, 3157, 3207, 3239
BpmI	CTGGAG	3 cut(s)	1524, 2793, 3177
Bpu10I	CCTNAGC	2 cut(s)	183, 470
Bpu1102I	GCTNAGC	1 cut(s)	1838
Bpu14I	TTCGAA	1 cut(s)	273
BpuEI	CTTGAG	2 cut(s)	1206, 1994
Bsa29I	ATCGAT	1 cut(s)	688
BsaAI	YACGTR	1 cut(s)	2520
BsaBI	GATNNNNATC	3 cut(s)	831, 1246, 2037
BsaHI	GRCGYC	1 cut(s)	2301
BsaJI	CCNNGG	6 cut(s)	1584, 1610, 2821, 2833, 3046, 3074
BsaWI	WCCGGW	3 cut(s)	376, 1944, 2001
BsaXI	ACNNNNNCTCC	8 cut(s)	880, 910, 1497, 1527, 2905, 2935, 3034, 3064
Bsc4I	CCNNNNNNNGG	5 cut(s)	236, 1024, 1266, 2853, 3039
Bse3DI	GCAATG	1 cut(s)	618
Bse8I	GATNNNNATC	3 cut(s)	831, 1246, 2037
BseAI	TCCGGA	3 cut(s)	376, 1944, 2001
BseBI	CCWGG	5 cut(s)	870, 1503, 1586, 2822, 3076
BseCI	ATCGAT	1 cut(s)	688
BseDI	CCNNGG	6 cut(s)	1584, 1610, 2821, 2833, 3046, 3074
BseJI	GATNNNNATC	3 cut(s)	831, 1246, 2037
BseLI	CCNNNNNNNGG	5 cut(s)	236, 1024, 1266, 2853, 3039
BseMI	GCAATG	1 cut(s)	618
BseMII	CTCAG	8 cut(s)	84, 174, 311, 551, 1469, 2057, 2076, 2231
BseRI	GAGGAG	2 cut(s)	100, 901
BseSI	GKGCMC	3 cut(s)	1312, 2104, 2519
BseXI	GCAGC	7 cut(s)	1057, 1089, 1183, 1186, 1633, 2072, 2639
BseYI	CCCAGC	1 cut(s)	2642
BsgI	GTGCAG	1 cut(s)	818
BshFI	GGCC	4 cut(s)	1691, 1884, 2199, 2832
BshNI	GGYRCC	1 cut(s)	850
BshVI	ATCGAT	1 cut(s)	688
BsiHKAI	GWGCWC	4 cut(s)	726, 2061, 2489, 2519
BsiHKCI	CYCGRG	1 cut(s)	3014
BsiSI	CCGG	4 cut(s)	377, 1945, 2002, 2763
BslFI	GGGAC	3 cut(s)	382, 589, 2967
BslI	CCNNNNNNNGG	5 cut(s)	236, 1024, 1266, 2853, 3039
BsmAI	GTCTC	4 cut(s)	300, 2012, 2694, 2908
BsmFI	GGGAC	3 cut(s)	382, 589, 2967
BsnI	GGCC	4 cut(s)	1691, 1884, 2199, 2832
BsoBI	CYCGRG	1 cut(s)	3014
Bsp119I	TTCGAA	1 cut(s)	273
Bsp1286I	GDGCHC	6 cut(s)	726, 1312, 2061, 2104, 2489, 2519
Bsp13I	TCCGGA	3 cut(s)	376, 1944, 2001
Bsp1720I	GCTNAGC	1 cut(s)	1838
Bsp19I	CCATGG	1 cut(s)	2833
BspACI	CCGC	4 cut(s)	280, 573, 1411, 2969
BspANI	GGCC	4 cut(s)	1691, 1884, 2199, 2832
BspCNI	CTCAG	8 cut(s)	85, 175, 310, 550, 1468, 2056, 2075, 2230
BspDI	ATCGAT	1 cut(s)	688
BspEI	TCCGGA	3 cut(s)	376, 1944, 2001
BspHI	TCATGA	1 cut(s)	3130
BspLI	GGNNCC	5 cut(s)	203, 258, 852, 1359, 3044
BspMAI	CTGCAG	4 cut(s)	1107, 1623, 1689, 2354
BspPI	GGATC	8 cut(s)	91, 693, 774, 827, 1046, 1815, 2475, 3110
BspQI	GCTCTTC	1 cut(s)	2494
BspT104I	TTCGAA	1 cut(s)	273
BspT107I	GGYRCC	1 cut(s)	850
BsrBI	CCGCTC	1 cut(s)	573
BsrDI	GCAATG	1 cut(s)	618
BssECI	CCNNGG	6 cut(s)	1584, 1610, 2821, 2833, 3046, 3074
BssNI	GRCGYC	1 cut(s)	2301
BssT1I	CCWWGG	2 cut(s)	2833, 3046
Bst2UI	CCWGG	5 cut(s)	870, 1503, 1586, 2822, 3076
Bst6I	CTCTTC	2 cut(s)	1439, 2494
BstACI	GRCGYC	1 cut(s)	2301
BstAPI	GCANNNNNTGC	1 cut(s)	2349
BstBAI	YACGTR	1 cut(s)	2520
BstBI	TTCGAA	1 cut(s)	273
BstC8I	GCNNGC	7 cut(s)	508, 546, 984, 1312, 1413, 1689, 2542
BstDSI	CCRYGG	1 cut(s)	2833
BstH2I	RGCGCY	2 cut(s)	93, 1667
BstHHI	GCGC	4 cut(s)	92, 1287, 1666, 2449
BstMAI	GTCTC	4 cut(s)	300, 2012, 2694, 2908
BstNI	CCWGG	5 cut(s)	870, 1503, 1586, 2822, 3076
BstNSI	RCATGY	3 cut(s)	510, 1151, 2540
BstPAI	GACNNNNGTC	1 cut(s)	1892
BstSCI	CCNGG	5 cut(s)	868, 1501, 1584, 2820, 3074
BstSFI	CTRYAG	5 cut(s)	1103, 1619, 1685, 2350, 2677
BstSLI	GKGCMC	3 cut(s)	1312, 2104, 2519
BstV1I	GCAGC	7 cut(s)	1057, 1089, 1183, 1186, 1633, 2072, 2639
BstV2I	GAAGAC	4 cut(s)	1746, 1914, 1926, 2078
BstX2I	RGATCY	5 cut(s)	96, 1051, 1807, 2467, 2653
BstXI	CCANNNNNNTGG	2 cut(s)	392, 2850
BstYI	RGATCY	5 cut(s)	96, 1051, 1807, 2467, 2653
Bsu15I	ATCGAT	1 cut(s)	688
BsuI	GTATCC	1 cut(s)	876
BsuRI	GGCC	4 cut(s)	1691, 1884, 2199, 2832
BsuTUI	ATCGAT	1 cut(s)	688
BtgI	CCRYGG	1 cut(s)	2833
BtgZI	GCGATG	2 cut(s)	837, 1831
BtsI	GCAGTG	4 cut(s)	806, 1564, 1682, 2359
Cac8I	GCNNGC	7 cut(s)	508, 546, 984, 1312, 1413, 1689, 2542
CaiI	CAGNNNCTG	3 cut(s)	226, 2141, 2512
CciI	TCATGA	1 cut(s)	3130
CfoI	GCGC	4 cut(s)	92, 1287, 1666, 2449
Cfr13I	GGNCC	3 cut(s)	56, 2198, 2710
ClaI	ATCGAT	1 cut(s)	688
Csp6I	GTAC	1 cut(s)	1119
CspCI	CAANNNNNGTGG	2 cut(s)	825, 860
CviQI	GTAC	1 cut(s)	1119
DraI	TTTAAA	1 cut(s)	1048
DraIII	CACNNNGTG	1 cut(s)	1266
DrdI	GACNNNNNNGTC	1 cut(s)	1168
DseDI	GACNNNNNNGTC	1 cut(s)	1168
Eam1104I	CTCTTC	2 cut(s)	1439, 2494
EarI	CTCTTC	2 cut(s)	1439, 2494
Ecl136II	GAGCTC	3 cut(s)	724, 2059, 2487
Eco130I	CCWWGG	2 cut(s)	2833, 3046
Eco147I	AGGCCT	1 cut(s)	1691
Eco24I	GRGCYC	3 cut(s)	726, 2061, 2489
Eco47I	GGWCC	2 cut(s)	56, 2710
Eco47III	AGCGCT	2 cut(s)	91, 1665
Eco53kI	GAGCTC	3 cut(s)	724, 2059, 2487
Eco57I	CTGAAG	7 cut(s)	803, 834, 1420, 1884, 2232, 2475, 3006
Eco72I	CACGTG	1 cut(s)	2520
Eco88I	CYCGRG	1 cut(s)	3014
EcoICRI	GAGCTC	3 cut(s)	724, 2059, 2487
EcoRI	GAATTC	2 cut(s)	416, 625
EcoRII	CCWGG	5 cut(s)	868, 1501, 1584, 2820, 3074
EcoT14I	CCWWGG	2 cut(s)	2833, 3046
EcoT22I	ATGCAT	2 cut(s)	620, 1674
EcoT38I	GRGCYC	3 cut(s)	726, 2061, 2489
ErhI	CCWWGG	2 cut(s)	2833, 3046
FaqI	GGGAC	3 cut(s)	382, 589, 2967
FauI	CCCGC	2 cut(s)	566, 1404
FbaI	TGATCA	4 cut(s)	697, 2035, 2224, 2527
Fnu4HI	GCNGC	7 cut(s)	1071, 1103, 1197, 1200, 1622, 2086, 2628
FriOI	GRGCYC	3 cut(s)	726, 2061, 2489
Fsp4HI	GCNGC	7 cut(s)	1071, 1103, 1197, 1200, 1622, 2086, 2628
GlaI	GCGC	4 cut(s)	91, 1286, 1665, 2448
GluI	GCNGC	7 cut(s)	1071, 1103, 1197, 1200, 1622, 2086, 2628
GsaI	CCCAGC	1 cut(s)	2646
GsuI	CTGGAG	3 cut(s)	1524, 2793, 3177
HaeII	RGCGCY	2 cut(s)	93, 1667
HaeIII	GGCC	4 cut(s)	1691, 1884, 2199, 2832
HapII	CCGG	4 cut(s)	377, 1945, 2002, 2763
HhaI	GCGC	4 cut(s)	92, 1287, 1666, 2449
Hin1I	GRCGYC	1 cut(s)	2301
Hin6I	GCGC	4 cut(s)	90, 1285, 1664, 2447
HinP1I	GCGC	4 cut(s)	90, 1285, 1664, 2447
HindIII	AAGCTT	1 cut(s)	737
HpaII	CCGG	4 cut(s)	377, 1945, 2002, 2763
Hpy166II	GTNNAC	4 cut(s)	718, 2517, 2737, 2797
Hpy8I	GTNNAC	4 cut(s)	718, 2517, 2737, 2797
HpyAV	CCTTC	9 cut(s)	270, 770, 1263, 1325, 1874, 1942, 2424, 2458, 3092
HpyCH4IV	ACGT	5 cut(s)	1760, 2116, 2301, 2519, 2670
HpySE526I	ACGT	5 cut(s)	1760, 2116, 2301, 2519, 2670
Hsp92I	GRCGYC	1 cut(s)	2301
HspAI	GCGC	4 cut(s)	90, 1285, 1664, 2447
Kpn2I	TCCGGA	3 cut(s)	376, 1944, 2001
Ksp22I	TGATCA	4 cut(s)	697, 2035, 2224, 2527
LguI	GCTCTTC	1 cut(s)	2494
LmnI	GCTCC	4 cut(s)	87, 207, 2812, 3042
Lsp1109I	GCAGC	7 cut(s)	1057, 1089, 1183, 1186, 1633, 2072, 2639
LweI	GCATC	8 cut(s)	169, 307, 360, 484, 1051, 1232, 1963, 3079
MaeII	ACGT	5 cut(s)	1760, 2116, 2301, 2519, 2670
MaeIII	GTNAC	5 cut(s)	1535, 1705, 2152, 3009, 3167
MbiI	CCGCTC	1 cut(s)	573
MfeI	CAATTG	3 cut(s)	693, 915, 1074
MflI	RGATCY	5 cut(s)	96, 1051, 1807, 2467, 2653
MhlI	GDGCHC	6 cut(s)	726, 1312, 2061, 2104, 2489, 2519
MlyI	GAGTC	1 cut(s)	2014
MmeI	TCCRAC	3 cut(s)	1909, 2688, 2871
Mph1103I	ATGCAT	2 cut(s)	620, 1674
MroI	TCCGGA	3 cut(s)	376, 1944, 2001
MroXI	GAANNNNTTC	1 cut(s)	1743
MseI	TTAA	9 cut(s)	170, 735, 1047, 1236, 1757, 2168, 2375, 2456, 2553
MslI	CAYNNNNRTG	2 cut(s)	1152, 2664
MspA1I	CMGCKG	3 cut(s)	1411, 1634, 2192
MspI	CCGG	4 cut(s)	377, 1945, 2002, 2763
MspR9I	CCNGG	5 cut(s)	870, 1503, 1586, 2822, 3076
MunI	CAATTG	3 cut(s)	693, 915, 1074
MvaI	CCWGG	5 cut(s)	870, 1503, 1586, 2822, 3076
NcoI	CCATGG	1 cut(s)	2833
NlaIV	GGNNCC	5 cut(s)	203, 258, 852, 1359, 3044
NmuCI	GTSAC	4 cut(s)	1535, 1705, 2152, 3167
NsiI	ATGCAT	2 cut(s)	620, 1674
NspI	RCATGY	3 cut(s)	510, 1151, 2540
NspV	TTCGAA	1 cut(s)	273
PaeI	GCATGC	1 cut(s)	510
PaeR7I	CTCGAG	1 cut(s)	3014
PagI	TCATGA	1 cut(s)	3130
PceI	AGGCCT	1 cut(s)	1691
PciSI	GCTCTTC	1 cut(s)	2494
PdmI	GAANNNNTTC	1 cut(s)	1743
PflMI	CCANNNNNTGG	2 cut(s)	1024, 1266
PfoI	TCCNGGA	1 cut(s)	868
PkrI	GCNGC	7 cut(s)	1072, 1104, 1198, 1201, 1623, 2087, 2629
PleI	GAGTC	1 cut(s)	2013
PmaCI	CACGTG	1 cut(s)	2520
PmlI	CACGTG	1 cut(s)	2520
PpsI	GAGTC	1 cut(s)	2013
Ppu21I	YACGTR	1 cut(s)	2520
PshAI	GACNNNNGTC	1 cut(s)	1892
PsiI	TTATAA	1 cut(s)	2702
Psp124BI	GAGCTC	3 cut(s)	726, 2061, 2489
Psp6I	CCWGG	5 cut(s)	868, 1501, 1584, 2820, 3074
PspCI	CACGTG	1 cut(s)	2520
PspFI	CCCAGC	1 cut(s)	2642
PspGI	CCWGG	5 cut(s)	868, 1501, 1584, 2820, 3074
PspN4I	GGNNCC	5 cut(s)	203, 258, 852, 1359, 3044
PspPI	GGNCC	3 cut(s)	56, 2198, 2710
PspXI	VCTCGAGB	1 cut(s)	3014
PsrI	GAACNNNNNNTAC	2 cut(s)	865, 897
PstI	CTGCAG	4 cut(s)	1107, 1623, 1689, 2354
PstNI	CAGNNNCTG	3 cut(s)	226, 2141, 2512
PsuI	RGATCY	5 cut(s)	96, 1051, 1807, 2467, 2653
PvuII	CAGCTG	2 cut(s)	1634, 2192
RsaI	GTAC	1 cut(s)	1120
RsaNI	GTAC	1 cut(s)	1119
RseI	CAYNNNNRTG	2 cut(s)	1152, 2664
SacI	GAGCTC	3 cut(s)	726, 2061, 2489
SapI	GCTCTTC	1 cut(s)	2494
SaqAI	TTAA	9 cut(s)	170, 735, 1047, 1236, 1757, 2168, 2375, 2456, 2553
SatI	GCNGC	7 cut(s)	1071, 1103, 1197, 1200, 1622, 2086, 2628
Sau96I	GGNCC	3 cut(s)	56, 2198, 2710
SchI	GAGTC	1 cut(s)	2014
ScrFI	CCNGG	5 cut(s)	870, 1503, 1586, 2822, 3076
SduI	GDGCHC	6 cut(s)	726, 1312, 2061, 2104, 2489, 2519
SfaNI	GCATC	8 cut(s)	169, 307, 360, 484, 1051, 1232, 1963, 3079
SfcI	CTRYAG	5 cut(s)	1103, 1619, 1685, 2350, 2677
Sfr274I	CTCGAG	1 cut(s)	3014
SfuI	TTCGAA	1 cut(s)	273
SinI	GGWCC	2 cut(s)	56, 2710
SlaI	CTCGAG	1 cut(s)	3014
SmiMI	CAYNNNNRTG	2 cut(s)	1152, 2664
SmlI	CTYRAG	3 cut(s)	1221, 2009, 3014
SmoI	CTYRAG	3 cut(s)	1221, 2009, 3014
SphI	GCATGC	1 cut(s)	510
SseBI	AGGCCT	1 cut(s)	1691
SsiI	CCGC	4 cut(s)	280, 573, 1411, 2969
SspI	AATATT	1 cut(s)	2278
SstI	GAGCTC	3 cut(s)	726, 2061, 2489
StuI	AGGCCT	1 cut(s)	1691
StyD4I	CCNGG	5 cut(s)	868, 1501, 1584, 2820, 3074
StyI	CCWWGG	2 cut(s)	2833, 3046
TaiI	ACGT	5 cut(s)	1763, 2119, 2304, 2522, 2673
TaqII	GACCGA	1 cut(s)	1166
Tru1I	TTAA	9 cut(s)	170, 735, 1047, 1236, 1757, 2168, 2375, 2456, 2553
Tru9I	TTAA	9 cut(s)	170, 735, 1047, 1236, 1757, 2168, 2375, 2456, 2553
TseFI	GTSAC	4 cut(s)	1535, 1705, 2152, 3167
TseI	GCWGC	7 cut(s)	1070, 1102, 1196, 1199, 1621, 2085, 2627
Tsp45I	GTSAC	4 cut(s)	1535, 1705, 2152, 3167
TspDTI	ATGAA	7 cut(s)	204, 1125, 1688, 2619, 2869, 3039, 3069
TspGWI	ACGGA	5 cut(s)	194, 873, 1684, 2090, 2562
Van91I	CCANNNNNTGG	2 cut(s)	1024, 1266
VneI	GTGCAC	1 cut(s)	2515
VpaK11BI	GGWCC	2 cut(s)	56, 2710
XbaI	TCTAGA	2 cut(s)	1336, 2317
XceI	RCATGY	3 cut(s)	510, 1151, 2540
XcmI	CCANNNNNNNNNTGG	3 cut(s)	843, 2850, 2907
XhoI	CTCGAG	1 cut(s)	3014
XmnI	GAANNNNTTC	1 cut(s)	1743
ZraI	GACGTC	1 cut(s)	2302
Zsp2I	ATGCAT	2 cut(s)	620, 1674

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.