Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0006775

Physical Location & Seq

Genomic Coordinates Reverse (-)

5521044 .. 5526982

Gene Structure 5939 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G005790.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2436 bp

ATGGTTCAGCGATTGGGGCGACAATTGCAGCGAGAAGAAGAGAAGAGAAGCACGAGCGGTTTAGCGATTGGGAACGAGGGGAGTATTGGTGATTCGATTGCTGAGTCAATTGGAGTTGTTGCCAACCCTGAAATTGTGGTTCTGGAGCTTACACAGAATCATCCTTTCTTCGTCCTTGCTAGTGATGGAGTGTTTGAGTTTATTTCTAGCCAAACCGTGGTTGATATGGTTGCAAAATATAAAGATCCACGTGATGCTTGTGCTGCAATTGTTGCCGAATCTTATAAACTCTGGCTGCAGTATGAAACCCGTACAGATGATATTACAGTGATCGTGGTGCATGTAGATGGGCTAACTGCTACTGCTGTTGGTCAATCAGTACATCCTGGTTCTGTTTTACGGCCACCTGTTCCTCAAGTTGTAGAGGTTACAGGATCAGAATCTCCTTCAACCATTAGCTGGAACTCAAGGAACCCTCGCATAAAACATGATTTGTCAAAGGCACGTCTTCGTGTGATTGAAAATTCTCTAGAAAACGGGCAAGTTTGGGTTCCTCCGCCTCCAGCCCACAGAAAAACCTGGGAGGAAGAAGCACAAATTGAGCGAGCTTTACATGATCATTTCCTCTTCAGAAAACTTACCGACTCTCAGTGTCATGTTTTATTGGATTGCATGCAAAGGGTTGAGGTCCAGCCTGGAGACATTGTTGTTAGACAGGGTGGTGAAGGTGACTGCTTTTATGTTGTTGGCAGTGGAGAATTTGAGGTGTCGGCAACCCAGGAAGAAAAGAACGGAGAGGTTCCGAGGGTTTTGCAACGATATACAGCTGACAAACTGTCATCCTTTGGAGAACTAGCACTAATGTATAACAAACCACTCCAGGCATCCGTTCGTGCTGTCACCACTGGAACTCTCTGGGCTTTAAGAAGAGAAGACTTTCGTGGAATTCTAATGTCAGAGTTTTCTAATTTGTCATATTTAAAGTTGCTTCGATCAGTGGATCTTTTATCCAGGTTGACAATATTACAGCTCAGCCATATTGCTGATTCTCTTTCTGAGGTTTCATTCTCAGATGGGCAAACAATTGTCAATGAGAATGAAGGCCTATTGGCATTGTACATTATCCAGAAGGGAAAAGTGAGGATTACAGTTGACGCAAGTTCAGTTAGCAATCCAGTTGTCTGCAGTCTCATGTCTGACAATCAAAAACAAGGTGATAATCCTCAGAGCGGTAAAGAGATCATAGTGGAGAAGACAGAGGGAAGCTACTTTGGTGAATGGACACTTCTCGGGGAACATATTGATTTGTTTGATGCAGTTGCTGTGGGGGATGTTGTTTGTGCTGTTTTGACGAAGGAAAAATTCGATTCAGTCATTGGCCCAATGACAAAGCTTACTCAGGATGATCAGAAATCAAGGGATCAATCATCGGAAACTTTAATGGAATCTGTTAAAAGTATTGATATCTCTGCTCTTACTAAAGTTCAGCTTGCTGATATGGAGTGGAGAAAGTGTCTGTATTCTACTGACTGCAGTGAGATTGGGCTTGTTCTTTTAAGAGATCCAGAAAATTTGCTTAGTTTGAAAAGGTTTTCAAAGAAGAAGGTCAGAAAGCTTGGAAAGGAAGCACAAGTATTAAAAGAGAAGGATCTAATCAAGAGCATAAGCCCTGCAGCTTGTGTGCCACAGGTTCTATCCACTTGTGTTGATCAGACACATGCTGCAATACTGCTCAATACATGCATTGCTTGTCCATTGGCCTCGATACTTCGCACGCCCCTTGATGAACCGTCTGCCCAATTTTGTACAGCCTCTCTAATTACTGCTTTGGAGGATCTCCACAAGAATGACGTTCTCTACAGAGGCTTGTCTCCTGATGCATTAATGTTGGACCACAAGGGACATTTACAGATTGTAGACTTCAGATTTGGGAAAAAGTTGTCTGGCCAAAGAACATATACAATTTGTGGAATGGCAGACTTTTTAGCTCCAGAGGTAGTTCAGGGAATAGGCCATGGTTTCCCAGCTGACTGGTGGGCATTGGGAGTCTTGATATATTTCATGTTACAAGGTGAATTGCCTTTTGGGTCATGGAGAGAAAGTGAGCTTGATATCTTTGCAAAGATTGCGAAGGGACAGCTTAATCTTCCTCAAACTTTCAGCCCTGCAGTTGTTGATCTCATTACCAAGTTACTTGAGGTTGATGAAAACACCAGACTAGGAAGCCAAGGTTGTGATTCTGTTAAAAGTCATCCCTGGTTCGATGGTATTGATTGGAAAGGGATTAGAGACTCTAGTTTTCCAGTTCCTCAGGAGATCACCTCTCGCATAGCTCAACATTTGGAAAGTCACTCAGATGAGTATAGTGCTCCTCAAGGTTCTCCGTCACACAATGAAGAGGAACTTGACATTCCGGAATGGTTTGATGACTGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko04022 ko04540 ko04611 ko04713 ko04714 ko04730 ko04740 ko04923 ko04924 ko04970 map04022 map04540 map04611 map04713 map04714 map04730 map04740 map04923 map04924 map04970

Pfam Domains

Protein Families

PP2C Pkinase cNMP_binding

Protein Analysis

811

Amino Acids

90.02

Weight (kDa)

5.07

Isoelectric Point (pI)

42.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PP2C	PF00481	30 - 93	2.2e-08	Protein phosphatase 2C
cNMP_binding	PF00027	228 - 319	5.9e-16	Cyclic nucleotide-binding domain
Pkinase	PF00069	520 - 754	1.1e-36	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	541 - 740	1.3e-10	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006775)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G20050 AT2G20050
fragaria_vesca	FvH4_1g05740 FvH4_1g05740 FvH4_1g05740
malus_domestica	MD02G1058500.v1.1 MD15G1192800.v1.1
prunus_persica	Prupe.7G224500_v2.0.a1 Prupe.7G224500_v2.0.a1 Prupe.7G224500_v2.0.a1 Prupe.7G224500_v2.0.a1
pyrus_communis	pycom02g04740 pycom15g17250 pycom15g17260
rosa_chinensis	RchiOBHm_Chr2g0091381
rosa_laevigata	RLG00000016219
rosa_multiflora	Rmu_ssc0000333.1_g000004 Rmu_ssc0000333.1_g000011
rosa_roxburghii	Rroxscaffold_2G00150130
rosa_rugosa	Rorug02G0017200 Rorug02G0017300
rosa_samantha	Rh2AG062300 Rh2BG061300 Rh2CG063400 Rh2DG061900
rosa_wichuraiana	Rw2G005790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	285
AccB7I	CCANNNNNTGG	2 cut(s)	217, 459
AccBSI	CCGCTC	2 cut(s)	57, 1230
AccI	GTMKAC	1 cut(s)	1917
AccIII	TCCGGA	1 cut(s)	2413
AciI	CCGC	3 cut(s)	57, 557, 1230
AclWI	GGATC	7 cut(s)	239, 442, 1008, 1428, 1556, 1656, 1842
AcoI	YGGCCR	2 cut(s)	401, 1945
AcsI	RAATTY	5 cut(s)	523, 758, 945, 1361, 1570
AcuI	CTGAAG	2 cut(s)	613, 1906
AcvI	CACGTG	1 cut(s)	251
AfaI	GTAC	4 cut(s)	313, 381, 1118, 1807
AfiI	CCNNNNNNNGG	3 cut(s)	217, 459, 1229
AgsI	TTSAA	4 cut(s)	450, 521, 1585, 1596
AhdI	GACNNNNNGTC	1 cut(s)	835
AjiI	CACGTC	1 cut(s)	506
AjnI	CCWGG	7 cut(s)	385, 578, 694, 777, 879, 1010, 2255
AjuI	GAANNNNNNNTTGG	2 cut(s)	2067, 2099
Alw21I	GWGCWC	1 cut(s)	2371
Alw26I	GTCTC	4 cut(s)	693, 1193, 1875, 2283
AlwI	GGATC	7 cut(s)	239, 442, 1008, 1428, 1556, 1656, 1842
AlwNI	CAGNNNCTG	1 cut(s)	1322
Ama87I	CYCGRG	1 cut(s)	1289
Aor13HI	TCCGGA	1 cut(s)	2413
AoxI	GGCC	6 cut(s)	401, 1102, 1378, 1758, 1945, 2011
ApeKI	GCWGC	5 cut(s)	28, 263, 295, 1673, 1721
ApoI	RAATTY	5 cut(s)	523, 758, 945, 1361, 1570
ArsI	GACNNNNNNTTYG	2 cut(s)	1910, 1942
AseI	ATTAAT	1 cut(s)	1883
Asp700I	GAANNNNTTC	1 cut(s)	936
AspS9I	GGNCC	3 cut(s)	688, 1379, 1891
AsuHPI	GGTGA	8 cut(s)	101, 734, 740, 892, 1226, 1286, 2084, 2311
AvaI	CYCGRG	1 cut(s)	1289
AvaII	GGWCC	2 cut(s)	688, 1891
AxyI	CCTNAGG	1 cut(s)	2310
BalI	TGGCCA	1 cut(s)	1947
BauI	CACGAG	1 cut(s)	52
BbrPI	CACGTG	1 cut(s)	251
BbsI	GAAGAC	3 cut(s)	500, 939, 1259
Bbv12I	GWGCWC	1 cut(s)	2371
BbvI	GCAGC	5 cut(s)	40, 250, 282, 1685, 1708
BccI	CCATC	4 cut(s)	179, 341, 1067, 2258
BceAI	ACGGC	1 cut(s)	416
BcgI	CGANNNNNNTGC	2 cut(s)	793, 827
BciT130I	CCWGG	7 cut(s)	387, 580, 696, 779, 881, 1012, 2257
BclI	TGATCA	3 cut(s)	616, 1405, 1708
BcoDI	GTCTC	4 cut(s)	693, 1193, 1875, 2283
BfaI	CTAG	6 cut(s)	180, 207, 530, 854, 2219, 2295
BfmI	CTRYAG	6 cut(s)	296, 1183, 1531, 1671, 1858, 2166
BisI	GCNGC	5 cut(s)	29, 264, 296, 1674, 1722
BlpI	GCTNAGC	1 cut(s)	1031
BlsI	GCNGC	5 cut(s)	30, 265, 297, 1675, 1723
Bme1390I	CCNGG	7 cut(s)	387, 580, 696, 779, 881, 1012, 2257
Bme18I	GGWCC	2 cut(s)	688, 1891
BmeRI	GACNNNNNGTC	1 cut(s)	835
BmeT110I	CYCGRG	1 cut(s)	1289
BmgBI	CACGTC	1 cut(s)	506
BmgT120I	GGNCC	3 cut(s)	688, 1379, 1891
BmiI	GGNNCC	3 cut(s)	473, 552, 801
BmrFI	CCNGG	7 cut(s)	387, 580, 696, 779, 881, 1012, 2257
BmsI	GCATC	4 cut(s)	244, 893, 1303, 1867
BpiI	GAAGAC	3 cut(s)	500, 939, 1259
BplI	GAGNNNNNCTC	2 cut(s)	2306, 2338
BpmI	CTGGAG	5 cut(s)	164, 546, 717, 863, 1974
Bpu1102I	GCTNAGC	1 cut(s)	1031
BpuEI	CTTGAG	4 cut(s)	399, 451, 2216, 2358
BsaAI	YACGTR	1 cut(s)	251
BsaBI	GATNNNNATC	1 cut(s)	439
BsaJI	CCNNGG	7 cut(s)	216, 579, 777, 803, 2014, 2227, 2255
BsaWI	WCCGGW	1 cut(s)	2413
BsaXI	ACNNNNNCTCC	8 cut(s)	73, 103, 690, 720, 1498, 1528, 2086, 2116
Bsc4I	CCNNNNNNNGG	3 cut(s)	217, 459, 1229
Bse1I	ACTGG	5 cut(s)	910, 1175, 2036, 2303, 2435
Bse21I	CCTNAGG	1 cut(s)	2310
Bse3DI	GCAATG	1 cut(s)	1743
Bse8I	GATNNNNATC	1 cut(s)	439
BseAI	TCCGGA	1 cut(s)	2413
BseBI	CCWGG	7 cut(s)	387, 580, 696, 779, 881, 1012, 2257
BseDI	CCNNGG	7 cut(s)	216, 579, 777, 803, 2014, 2227, 2255
BseGI	GGATG	7 cut(s)	160, 382, 839, 884, 1336, 1408, 2251
BseJI	GATNNNNATC	1 cut(s)	439
BseLI	CCNNNNNNNGG	3 cut(s)	217, 459, 1229
BseMI	GCAATG	1 cut(s)	1743
BseMII	CTCAG	9 cut(s)	93, 662, 1045, 1047, 1083, 1238, 1412, 2324, 2367
BseNI	ACTGG	5 cut(s)	910, 1175, 2036, 2303, 2435
BseRI	GAGGAG	1 cut(s)	2361
BseXI	GCAGC	5 cut(s)	40, 250, 282, 1685, 1708
BseYI	CCCAGC	1 cut(s)	2023
BshFI	GGCC	6 cut(s)	403, 1104, 1380, 1760, 1947, 2013
BsiHKAI	GWGCWC	1 cut(s)	2371
BsiHKCI	CYCGRG	1 cut(s)	1289
BsiSI	CCGG	1 cut(s)	2414
BslFI	GGGAC	2 cut(s)	1914, 2148
BslI	CCNNNNNNNGG	3 cut(s)	217, 459, 1229
BsmAI	GTCTC	4 cut(s)	693, 1193, 1875, 2283
BsmFI	GGGAC	2 cut(s)	1914, 2148
BsnI	GGCC	6 cut(s)	403, 1104, 1380, 1760, 1947, 2013
BsoBI	CYCGRG	1 cut(s)	1289
Bsp1286I	GDGCHC	1 cut(s)	2371
Bsp13I	TCCGGA	1 cut(s)	2413
Bsp1407I	TGTACA	2 cut(s)	1116, 1805
Bsp1720I	GCTNAGC	1 cut(s)	1031
Bsp19I	CCATGG	1 cut(s)	2014
BspACI	CCGC	3 cut(s)	57, 557, 1230
BspANI	GGCC	6 cut(s)	403, 1104, 1380, 1760, 1947, 2013
BspCNI	CTCAG	9 cut(s)	94, 661, 1044, 1048, 1082, 1237, 1411, 2323, 2366
BspEI	TCCGGA	1 cut(s)	2413
BspLI	GGNNCC	3 cut(s)	473, 552, 801
BspMAI	CTGCAG	5 cut(s)	300, 1187, 1535, 1675, 2170
BspPI	GGATC	7 cut(s)	239, 442, 1008, 1428, 1556, 1656, 1842
BsrBI	CCGCTC	2 cut(s)	57, 1230
BsrDI	GCAATG	1 cut(s)	1743
BsrGI	TGTACA	2 cut(s)	1116, 1805
BsrI	ACTGG	5 cut(s)	910, 1175, 2036, 2303, 2435
BssECI	CCNNGG	7 cut(s)	216, 579, 777, 803, 2014, 2227, 2255
BssSI	CACGAG	1 cut(s)	52
BssT1I	CCWWGG	2 cut(s)	2014, 2227
Bst2BI	CACGAG	1 cut(s)	52
Bst2UI	CCWGG	7 cut(s)	387, 580, 696, 779, 881, 1012, 2257
Bst4CI	ACNGT	5 cut(s)	217, 328, 837, 1150, 1791
Bst6I	CTCTTC	5 cut(s)	33, 38, 632, 922, 2391
BstAPI	GCANNNNNTGC	2 cut(s)	272, 2126
BstAUI	TGTACA	2 cut(s)	1116, 1805
BstBAI	YACGTR	1 cut(s)	251
BstC8I	GCNNGC	4 cut(s)	606, 674, 1491, 1775
BstDSI	CCRYGG	2 cut(s)	216, 2014
BstF5I	GGATG	7 cut(s)	160, 382, 839, 884, 1336, 1408, 2251
BstMAI	GTCTC	4 cut(s)	693, 1193, 1875, 2283
BstMWI	GCNNNNNNNGC	5 cut(s)	16, 25, 263, 272, 2126
BstNI	CCWGG	7 cut(s)	387, 580, 696, 779, 881, 1012, 2257
BstNSI	RCATGY	4 cut(s)	344, 676, 1721, 1743
BstSCI	CCNGG	7 cut(s)	385, 578, 694, 777, 879, 1010, 2255
BstSFI	CTRYAG	6 cut(s)	296, 1183, 1531, 1671, 1858, 2166
BstV1I	GCAGC	5 cut(s)	40, 250, 282, 1685, 1708
BstV2I	GAAGAC	3 cut(s)	500, 939, 1259
BstX2I	RGATCY	5 cut(s)	244, 1000, 1561, 1648, 1834
BstXI	CCANNNNNNTGG	1 cut(s)	2031
BstYI	RGATCY	5 cut(s)	244, 1000, 1561, 1648, 1834
Bsu36I	CCTNAGG	1 cut(s)	2310
BsuRI	GGCC	6 cut(s)	403, 1104, 1380, 1760, 1947, 2013
BtgI	CCRYGG	2 cut(s)	216, 2014
BtrI	CACGTC	1 cut(s)	506
BtsCI	GGATG	7 cut(s)	160, 382, 839, 884, 1336, 1408, 2251
BtsI	GCAGTG	2 cut(s)	757, 1540
BtsIMutI	CAGTG	6 cut(s)	333, 656, 757, 903, 1002, 1540
Cac8I	GCNNGC	4 cut(s)	606, 674, 1491, 1775
CaiI	CAGNNNCTG	1 cut(s)	1322
Cfr13I	GGNCC	3 cut(s)	688, 1379, 1891
CseI	GACGC	1 cut(s)	1163
Csp6I	GTAC	4 cut(s)	312, 380, 1117, 1806
CspCI	CAANNNNNGTGG	2 cut(s)	1687, 1722
CviQI	GTAC	4 cut(s)	312, 380, 1117, 1806
DraI	TTTAAA	1 cut(s)	981
DriI	GACNNNNNGTC	1 cut(s)	835
EaeI	YGGCCR	2 cut(s)	401, 1945
Eam1104I	CTCTTC	5 cut(s)	33, 38, 632, 922, 2391
Eam1105I	GACNNNNNGTC	1 cut(s)	835
EarI	CTCTTC	5 cut(s)	33, 38, 632, 922, 2391
EciI	GGCGGA	1 cut(s)	546
Eco130I	CCWWGG	2 cut(s)	2014, 2227
Eco147I	AGGCCT	1 cut(s)	1104
Eco32I	GATATC	2 cut(s)	1465, 2113
Eco47I	GGWCC	2 cut(s)	688, 1891
Eco57I	CTGAAG	2 cut(s)	613, 1906
Eco72I	CACGTG	1 cut(s)	251
Eco81I	CCTNAGG	1 cut(s)	2310
Eco88I	CYCGRG	1 cut(s)	1289
EcoRI	GAATTC	1 cut(s)	945
EcoRII	CCWGG	7 cut(s)	385, 578, 694, 777, 879, 1010, 2255
EcoRV	GATATC	2 cut(s)	1465, 2113
EcoT14I	CCWWGG	2 cut(s)	2014, 2227
EcoT22I	ATGCAT	2 cut(s)	1745, 1882
ErhI	CCWWGG	2 cut(s)	2014, 2227
FalI	AAGNNNNNCTT	4 cut(s)	1473, 1505, 2388, 2420
FaqI	GGGAC	2 cut(s)	1914, 2148
FbaI	TGATCA	3 cut(s)	616, 1405, 1708
FblI	GTMKAC	1 cut(s)	1917
Fnu4HI	GCNGC	5 cut(s)	29, 264, 296, 1674, 1722
FokI	GGATG	7 cut(s)	147, 369, 826, 871, 1343, 1415, 2238
Fsp4HI	GCNGC	5 cut(s)	29, 264, 296, 1674, 1722
FspBI	CTAG	6 cut(s)	180, 207, 530, 854, 2219, 2295
GluI	GCNGC	5 cut(s)	29, 264, 296, 1674, 1722
GsaI	CCCAGC	1 cut(s)	2027
GsuI	CTGGAG	5 cut(s)	164, 546, 717, 863, 1974
HaeIII	GGCC	6 cut(s)	403, 1104, 1380, 1760, 1947, 2013
HapII	CCGG	1 cut(s)	2414
HgaI	GACGC	1 cut(s)	1163
HincII	GTYRAC	2 cut(s)	1017, 1153
HindII	GTYRAC	2 cut(s)	1017, 1153
HindIII	AAGCTT	2 cut(s)	1391, 1613
HpaII	CCGG	1 cut(s)	2414
HphI	GGTGA	8 cut(s)	101, 734, 740, 892, 1226, 1286, 2084, 2311
Hpy166II	GTNNAC	3 cut(s)	1017, 1153, 1918
Hpy8I	GTNNAC	3 cut(s)	1017, 1153, 1918
HpyAV	CCTTC	8 cut(s)	456, 719, 1094, 1123, 1348, 1597, 1639, 2125
HpyCH4III	ACNGT	5 cut(s)	217, 328, 837, 1150, 1791
HpyCH4IV	ACGT	3 cut(s)	250, 505, 1851
HpyF10VI	GCNNNNNNNGC	5 cut(s)	16, 25, 263, 272, 2126
HpySE526I	ACGT	3 cut(s)	250, 505, 1851
Kpn2I	TCCGGA	1 cut(s)	2413
Ksp22I	TGATCA	3 cut(s)	616, 1405, 1708
LmnI	GCTCC	3 cut(s)	145, 1993, 2374
Lsp1109I	GCAGC	5 cut(s)	40, 250, 282, 1685, 1708
LweI	GCATC	4 cut(s)	244, 893, 1303, 1867
MaeI	CTAG	6 cut(s)	180, 207, 530, 854, 2219, 2295
MaeII	ACGT	3 cut(s)	250, 505, 1851
MaeIII	GTNAC	7 cut(s)	427, 728, 898, 2064, 2190, 2348, 2385
MbiI	CCGCTC	2 cut(s)	57, 1230
MfeI	CAATTG	4 cut(s)	23, 108, 267, 1083
MflI	RGATCY	5 cut(s)	244, 1000, 1561, 1648, 1834
MhlI	GDGCHC	1 cut(s)	2371
MlsI	TGGCCA	1 cut(s)	1947
MluNI	TGGCCA	1 cut(s)	1947
MlyI	GAGTC	4 cut(s)	113, 638, 2055, 2285
MmeI	TCCRAC	1 cut(s)	1869
Mox20I	TGGCCA	1 cut(s)	1947
Mph1103I	ATGCAT	2 cut(s)	1745, 1882
MroI	TCCGGA	1 cut(s)	2413
MroXI	GAANNNNTTC	1 cut(s)	936
MscI	TGGCCA	1 cut(s)	1947
MseI	TTAA	9 cut(s)	923, 980, 1439, 1452, 1556, 1637, 1883, 2142, 2244
MslI	CAYNNNNRTG	3 cut(s)	345, 1845, 2355
Msp20I	TGGCCA	1 cut(s)	1947
MspA1I	CMGCKG	2 cut(s)	827, 2027
MspI	CCGG	1 cut(s)	2414
MspR9I	CCNGG	7 cut(s)	387, 580, 696, 779, 881, 1012, 2257
MunI	CAATTG	4 cut(s)	23, 108, 267, 1083
MvaI	CCWGG	7 cut(s)	387, 580, 696, 779, 881, 1012, 2257
MwoI	GCNNNNNNNGC	5 cut(s)	16, 25, 263, 272, 2126
NcoI	CCATGG	1 cut(s)	2014
NlaIV	GGNNCC	3 cut(s)	473, 552, 801
NmuCI	GTSAC	4 cut(s)	728, 898, 2348, 2385
NsiI	ATGCAT	2 cut(s)	1745, 1882
NspI	RCATGY	4 cut(s)	344, 676, 1721, 1743
PaeI	GCATGC	1 cut(s)	676
PceI	AGGCCT	1 cut(s)	1104
PdmI	GAANNNNTTC	1 cut(s)	936
PfeI	GAWTC	8 cut(s)	92, 157, 278, 440, 1046, 1367, 1445, 2237
PflMI	CCANNNNNTGG	2 cut(s)	217, 459
PkrI	GCNGC	5 cut(s)	30, 265, 297, 1675, 1723
PleI	GAGTC	4 cut(s)	112, 638, 2054, 2285
PmaCI	CACGTG	1 cut(s)	251
PmlI	CACGTG	1 cut(s)	251
PpsI	GAGTC	4 cut(s)	112, 638, 2054, 2285
Ppu21I	YACGTR	1 cut(s)	251
PshBI	ATTAAT	1 cut(s)	1883
PsiI	TTATAA	1 cut(s)	285
Psp6I	CCWGG	7 cut(s)	385, 578, 694, 777, 879, 1010, 2255
PspCI	CACGTG	1 cut(s)	251
PspFI	CCCAGC	1 cut(s)	2023
PspGI	CCWGG	7 cut(s)	385, 578, 694, 777, 879, 1010, 2255
PspN4I	GGNNCC	3 cut(s)	473, 552, 801
PspPI	GGNCC	3 cut(s)	688, 1379, 1891
PstI	CTGCAG	5 cut(s)	300, 1187, 1535, 1675, 2170
PstNI	CAGNNNCTG	1 cut(s)	1322
PsuI	RGATCY	5 cut(s)	244, 1000, 1561, 1648, 1834
PvuII	CAGCTG	2 cut(s)	827, 2027
RsaI	GTAC	4 cut(s)	313, 381, 1118, 1807
RsaNI	GTAC	4 cut(s)	312, 380, 1117, 1806
RseI	CAYNNNNRTG	3 cut(s)	345, 1845, 2355
SaqAI	TTAA	9 cut(s)	923, 980, 1439, 1452, 1556, 1637, 1883, 2142, 2244
SatI	GCNGC	5 cut(s)	29, 264, 296, 1674, 1722
Sau96I	GGNCC	3 cut(s)	688, 1379, 1891
SchI	GAGTC	4 cut(s)	113, 638, 2055, 2285
ScrFI	CCNGG	7 cut(s)	387, 580, 696, 779, 881, 1012, 2257
SduI	GDGCHC	1 cut(s)	2371
SfaNI	GCATC	4 cut(s)	244, 893, 1303, 1867
SfcI	CTRYAG	6 cut(s)	296, 1183, 1531, 1671, 1858, 2166
SinI	GGWCC	2 cut(s)	688, 1891
SmiMI	CAYNNNNRTG	3 cut(s)	345, 1845, 2355
SmlI	CTYRAG	4 cut(s)	414, 466, 2195, 2373
SmoI	CTYRAG	4 cut(s)	414, 466, 2195, 2373
SphI	GCATGC	1 cut(s)	676
SseBI	AGGCCT	1 cut(s)	1104
SsiI	CCGC	3 cut(s)	57, 557, 1230
SspI	AATATT	1 cut(s)	1023
SspMI	CTAG	6 cut(s)	180, 207, 530, 854, 2219, 2295
StuI	AGGCCT	1 cut(s)	1104
StyD4I	CCNGG	7 cut(s)	385, 578, 694, 777, 879, 1010, 2255
StyI	CCWWGG	2 cut(s)	2014, 2227
TaaI	ACNGT	5 cut(s)	217, 328, 837, 1150, 1791
TaiI	ACGT	3 cut(s)	253, 508, 1854
TaqI	TCGA	5 cut(s)	95, 991, 1365, 1763, 2262
TatI	WGTACW	3 cut(s)	379, 1116, 1805
TfiI	GAWTC	8 cut(s)	92, 157, 278, 440, 1046, 1367, 1445, 2237
Tru1I	TTAA	9 cut(s)	923, 980, 1439, 1452, 1556, 1637, 1883, 2142, 2244
Tru9I	TTAA	9 cut(s)	923, 980, 1439, 1452, 1556, 1637, 1883, 2142, 2244
TscAI	CASTG	6 cut(s)	333, 656, 757, 910, 1002, 1540
TseFI	GTSAC	4 cut(s)	728, 898, 2348, 2385
TseI	GCWGC	5 cut(s)	28, 263, 295, 1673, 1721
Tsp45I	GTSAC	4 cut(s)	728, 898, 2348, 2385
TspDTI	ATGAA	7 cut(s)	318, 1053, 1113, 1800, 2050, 2220, 2409
TspGWI	ACGGA	3 cut(s)	807, 877, 2373
TspRI	CASTG	6 cut(s)	333, 656, 757, 910, 1002, 1540
Van91I	CCANNNNNTGG	2 cut(s)	217, 459
VpaK11BI	GGWCC	2 cut(s)	688, 1891
VspI	ATTAAT	1 cut(s)	1883
XapI	RAATTY	5 cut(s)	523, 758, 945, 1361, 1570
XbaI	TCTAGA	1 cut(s)	529
XceI	RCATGY	4 cut(s)	344, 676, 1721, 1743
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2031
XmiI	GTMKAC	1 cut(s)	1917
XmnI	GAANNNNTTC	1 cut(s)	936
XspI	CTAG	6 cut(s)	180, 207, 530, 854, 2219, 2295
Zsp2I	ATGCAT	2 cut(s)	1745, 1882

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.