Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0002467

Physical Location & Seq

Genomic Coordinates Reverse (-)

8836018 .. 8838710

Gene Structure 2693 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT2G20510.3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1419 bp

ATGGCGATTAGAAAGATAATTCGAGATTTACTGATCACAAAGCAGCCTCTTTTACGTCAACTATTCCACCAACGAAGAGCAAACGCAAGATCAGAGTTTCTTCCTGCTATTGGGTATACGAGTCATCGCCGGTTCAGCGTTTTCACCGAGTTCTCGAAGAATATTAGAGGAGAAGCTCATAGCAATCCTGAATTTGAAAGAACAGTAAAGGAGCTCAAGGAAAGAACAGAAGAGTTTAAAGGTGTAACAGAGGACCTGAAAGTGAGAACGAAACAAACGACTGAAAAGCTTTACAAGCAAGCTGATGGTGTGTGGACTGAGGCTGAATCTGCGGCCAAAAAGGTCTCTTCAAGTGTGAAAGACAAGCTCTCAGCAGCCAGTGAAGAGGTCAAGGAGTCATTCAAGCTCGGGAAGGAGGAGAATGCAGAGTCAGCGAGTTCATCAGGCACAAGAGCCTCTCAAGGAGAAAAACAGCAATCAGGTTCTACGGAGGAACTACATACATTTTTTGCAAAATTCAAGTCAAGTCTTTCTTCACCAAAGGTATCCGAGGTGTTTTACAGGCTGAAGGAAGCCAAACCCTTTGATATAGTAAAGCAAGCACTTGACATTGTGAAGGATGAGTTACGTGGGAACCCATCTAGAAAAAAGTTCCTGGAACATACACCTCCCCCGCCATTCACCGGTGAGAGAAGTATGAGAACAGAGATGGTGGTTACGCAAACAAAACAGTCTAAGTTGCAACAAAAGTGGGAATCCTTTAGAGAAAAGATGCAAGGTTCTCCTGTATTTAAACGTCTAAGCGGAATGAGTGAGCCTGTTGTCAACAAGAGCCAAGAGATTGCAGAAGATGTGAGGGAAATATGGGAAACCAGTGATAATCCAATTGTTCACAAGATTCAGGACATGAATGAGAAGTTCTTGAAGGAAACAGATTCTGCATCAACTTACAAAGAGATACGCAGCCGAGACCCATCGTTCTCCTTGCCAGACTTTGCTGCAGAAATTGAGGAAGTCATCAAACCAGTCTTGAATGCATACAGTGAGGGAGATGTTGAGACCTTGAAGAAGTATTGCAGCAAGGAAGTGATTGAACGGTGCACGGCAGAGCGCACAGCTTACCAAACTCATGGTGTCCTTTTCGATAACAAGCTTCTGCATATATCTGAAGTCTCAGTTTCTGTGACAAAGATGATGGGAGATTCCCCAATAATCATCGCTAAGTTCCAAACGCAAGAAATCTACTGCGTTCGTGACGAGAATGGAGAAATCCAAGAAGGTGGGCAGGACACGATACACACGGTGTACCATGAATGGGCAATGCAACAAGTGGAGACAACAGAATTAGGGGAAGATGCTATTTATCCCATTTGGAGGCTCAGAGAGATGTGTAGAAATGGTGTTCAAGCTCTCATTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0005744 GO:0005759 GO:0006605 GO:0006626 GO:0006810 GO:0006839 GO:0006886 GO:0006996 GO:0007005 GO:0008104 GO:0008150 GO:0008320 GO:0008565 GO:0009987 GO:0015031 GO:0015399 GO:0015405 GO:0015440 GO:0015450 GO:0015462 GO:0015833 GO:0016020 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017038 GO:0017111 GO:0019866 GO:0022804 GO:0022857 GO:0022884 GO:0030150 GO:0031090 GO:0031966 GO:0031967 GO:0031974 GO:0031975 GO:0032991 GO:0033036 GO:0033220 GO:0033365 GO:0034613 GO:0042623 GO:0042626 GO:0042886 GO:0042887 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0044743 GO:0045184 GO:0046907 GO:0051087 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055085 GO:0065002 GO:0070013 GO:0070585 GO:0070727 GO:0071702 GO:0071705 GO:0071806 GO:0071840 GO:0072594 GO:0072655 GO:0098796 GO:0098798 GO:0098800 GO:1904680 GO:1990542

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Tim44

Protein Analysis

472

Amino Acids

54.09

Weight (kDa)

6.61

Isoelectric Point (pI)

52.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Tim44	PF04280	315 - 466	3.7e-39	Tim44-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002467)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G20510 AT2G20510 AT2G20510 AT2G20510 AT2G36070
fragaria_vesca	FvH4_2g21470 FvH4_2g21470 FvH4_4g12040 FvH4_4g12040 FvH4_4g12040 FvH4_4g12040
malus_domestica	MD05G1197100.v1.1 MD09G1008600.v1.1
prunus_persica	Prupe.8G223600_v2.0.a1
pyrus_communis	pycom05g18420 pycom10g15910
rosa_chinensis	RchiOBHm_Chr4g0409381 RchiOBHm_Chr5g0013581 RchiOBHm_Chr6g0288111
rosa_laevigata	RLG00000012438 RLG00000032032
rosa_multiflora	Rmu_co8365065.1_g000001 Rmu_sc0001616.1_g000049 Rmu_sc0001851.1_g000030 Rmu_sc0010766.1_g000003
rosa_roxburghii	Rroxscaffold_1G00062990 Rroxscaffold_5G00353910 Rroxscaffold_7G00180510
rosa_rugosa	Rorug04G0093400 Rorug06G0194200
rosa_samantha	Rh4AG155900 Rh4BG155100 Rh4CG165200 Rh4DG149000 Rh5BG102300 Rh6AG304400 Rh6BG310400 Rh6CG318500 Rh6DG303400
rosa_wichuraiana	Rw4G012760 Rw6G026340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	116
AciI	CCGC	3 cut(s)	332, 674, 804
AcoI	YGGCCR	1 cut(s)	333
AcsI	RAATTY	2 cut(s)	191, 515
AcuI	CTGAAG	2 cut(s)	587, 1188
AdeI	CACNNNGTG	1 cut(s)	1303
AfaI	GTAC	1 cut(s)	1307
AfiI	CCNNNNNNNGG	4 cut(s)	110, 683, 1315, 1374
AgeI	ACCGGT	1 cut(s)	683
AgsI	TTSAA	9 cut(s)	197, 351, 403, 520, 925, 1033, 1066, 1094, 1406
AjnI	CCWGG	1 cut(s)	654
AluBI	AGCT	9 cut(s)	176, 214, 289, 302, 367, 406, 1118, 1153, 1409
AluI	AGCT	9 cut(s)	176, 214, 289, 302, 367, 406, 1118, 1153, 1409
Alw21I	GWGCWC	2 cut(s)	216, 1103
Alw26I	GTCTC	5 cut(s)	349, 963, 1052, 1177, 1328
Alw44I	GTGCAC	1 cut(s)	1099
AlwNI	CAGNNNCTG	2 cut(s)	938, 1181
Ama87I	CYCGRG	1 cut(s)	407
AoxI	GGCC	1 cut(s)	333
ApaLI	GTGCAC	1 cut(s)	1099
ApeKI	GCWGC	5 cut(s)	43, 374, 963, 998, 1077
ApoI	RAATTY	2 cut(s)	191, 515
ArsI	GACNNNNNNTTYG	2 cut(s)	506, 538
AsiGI	ACCGGT	1 cut(s)	683
AspLEI	GCGC	1 cut(s)	1113
AspS9I	GGNCC	1 cut(s)	253
AsuHPI	GGTGA	4 cut(s)	136, 528, 673, 698
AvaI	CYCGRG	1 cut(s)	407
AvaII	GGWCC	1 cut(s)	253
BaeGI	GKGCMC	1 cut(s)	1103
BanII	GRGCYC	1 cut(s)	216
Bbv12I	GWGCWC	2 cut(s)	216, 1103
BbvI	GCAGC	5 cut(s)	55, 386, 975, 985, 1089
BccI	CCATC	5 cut(s)	299, 646, 703, 982, 1189
BceAI	ACGGC	1 cut(s)	1119
BciT130I	CCWGG	1 cut(s)	656
BciVI	GTATCC	1 cut(s)	556
BclI	TGATCA	1 cut(s)	33
BcoDI	GTCTC	5 cut(s)	349, 963, 1052, 1177, 1328
BfaI	CTAG	1 cut(s)	642
BfmI	CTRYAG	1 cut(s)	999
BfuI	GTATCC	1 cut(s)	556
BisI	GCNGC	6 cut(s)	44, 333, 375, 964, 999, 1078
BlsI	GCNGC	6 cut(s)	45, 334, 376, 965, 1000, 1079
Bme1390I	CCNGG	1 cut(s)	656
Bme18I	GGWCC	1 cut(s)	253
BmeT110I	CYCGRG	1 cut(s)	407
BmgT120I	GGNCC	1 cut(s)	253
BmiI	GGNNCC	1 cut(s)	635
BmrFI	CCNGG	1 cut(s)	656
BmsI	GCATC	3 cut(s)	762, 950, 1345
BpuEI	CTTGAG	2 cut(s)	200, 444
BsaAI	YACGTR	1 cut(s)	629
BsaI	GGTCTC	3 cut(s)	349, 963, 1052
BsaJI	CCNNGG	1 cut(s)	549
BsaWI	WCCGGW	1 cut(s)	683
Bsc4I	CCNNNNNNNGG	4 cut(s)	110, 683, 1315, 1374
Bse118I	RCCGGY	2 cut(s)	129, 683
Bse1I	ACTGG	3 cut(s)	378, 873, 1025
Bse3DI	GCAATG	1 cut(s)	1326
BseBI	CCWGG	1 cut(s)	656
BseDI	CCNNGG	1 cut(s)	549
BseGI	GGATG	1 cut(s)	625
BseLI	CCNNNNNNNGG	4 cut(s)	110, 683, 1315, 1374
BseMI	GCAATG	1 cut(s)	1326
BseMII	CTCAG	4 cut(s)	309, 384, 1188, 1393
BseNI	ACTGG	3 cut(s)	378, 873, 1025
BseRI	GAGGAG	2 cut(s)	183, 431
BseSI	GKGCMC	1 cut(s)	1103
BseXI	GCAGC	5 cut(s)	55, 386, 975, 985, 1089
BshFI	GGCC	1 cut(s)	335
BshTI	ACCGGT	1 cut(s)	683
BsiHKAI	GWGCWC	2 cut(s)	216, 1103
BsiHKCI	CYCGRG	1 cut(s)	407
BsiSI	CCGG	2 cut(s)	130, 684
BslI	CCNNNNNNNGG	4 cut(s)	110, 683, 1315, 1374
BsmAI	GTCTC	5 cut(s)	349, 963, 1052, 1177, 1328
BsmI	GAATGC	2 cut(s)	427, 1039
BsnI	GGCC	1 cut(s)	335
Bso31I	GGTCTC	3 cut(s)	349, 963, 1052
BsoBI	CYCGRG	1 cut(s)	407
Bsp1286I	GDGCHC	2 cut(s)	216, 1103
Bsp143I	GATC	2 cut(s)	33, 89
BspACI	CCGC	3 cut(s)	332, 674, 804
BspANI	GGCC	1 cut(s)	335
BspCNI	CTCAG	4 cut(s)	310, 383, 1187, 1392
BspLI	GGNNCC	1 cut(s)	635
BspMAI	CTGCAG	1 cut(s)	1003
BspQI	GCTCTTC	1 cut(s)	70
BspTNI	GGTCTC	3 cut(s)	349, 963, 1052
BsrDI	GCAATG	1 cut(s)	1326
BsrFI	RCCGGY	2 cut(s)	129, 683
BsrI	ACTGG	3 cut(s)	378, 873, 1025
BssAI	RCCGGY	2 cut(s)	129, 683
BssECI	CCNNGG	1 cut(s)	549
BssMI	GATC	2 cut(s)	33, 89
BssNAI	GTATAC	1 cut(s)	117
Bst1107I	GTATAC	1 cut(s)	117
Bst2UI	CCWGG	1 cut(s)	656
Bst4CI	ACNGT	5 cut(s)	205, 732, 1043, 1098, 1303
Bst6I	CTCTTC	4 cut(s)	70, 225, 352, 378
BstBAI	YACGTR	1 cut(s)	629
BstC8I	GCNNGC	2 cut(s)	300, 600
BstDEI	CTNAG	7 cut(s)	318, 370, 735, 800, 1174, 1221, 1379
BstF5I	GGATG	1 cut(s)	625
BstHHI	GCGC	1 cut(s)	1113
BstKTI	GATC	2 cut(s)	36, 92
BstMAI	GTCTC	5 cut(s)	349, 963, 1052, 1177, 1328
BstMBI	GATC	2 cut(s)	33, 89
BstMWI	GCNNNNNNNGC	4 cut(s)	135, 295, 329, 431
BstNI	CCWGG	1 cut(s)	656
BstSCI	CCNGG	1 cut(s)	654
BstSFI	CTRYAG	1 cut(s)	999
BstSLI	GKGCMC	1 cut(s)	1103
BstV1I	GCAGC	5 cut(s)	55, 386, 975, 985, 1089
BstXI	CCANNNNNNTGG	2 cut(s)	1130, 1280
BstZ17I	GTATAC	1 cut(s)	117
BsuI	GTATCC	1 cut(s)	556
BsuRI	GGCC	1 cut(s)	335
BtgZI	GCGATG	2 cut(s)	110, 1201
BtsCI	GGATG	1 cut(s)	625
BtsIMutI	CAGTG	3 cut(s)	385, 880, 1048
Cac8I	GCNNGC	2 cut(s)	300, 600
CaiI	CAGNNNCTG	2 cut(s)	938, 1181
CfoI	GCGC	1 cut(s)	1113
Cfr10I	RCCGGY	2 cut(s)	129, 683
Cfr13I	GGNCC	1 cut(s)	253
Csp6I	GTAC	1 cut(s)	1306
CspAI	ACCGGT	1 cut(s)	683
CspCI	CAANNNNNGTGG	2 cut(s)	731, 766
CviAII	CATG	3 cut(s)	907, 1130, 1310
CviQI	GTAC	1 cut(s)	1306
DdeI	CTNAG	7 cut(s)	318, 370, 735, 800, 1174, 1221, 1379
DpnI	GATC	2 cut(s)	35, 91
DpnII	GATC	2 cut(s)	33, 89
DraI	TTTAAA	2 cut(s)	238, 793
DraIII	CACNNNGTG	1 cut(s)	1303
EaeI	YGGCCR	1 cut(s)	333
Eam1104I	CTCTTC	4 cut(s)	70, 225, 352, 378
EarI	CTCTTC	4 cut(s)	70, 225, 352, 378
Ecl136II	GAGCTC	1 cut(s)	214
Eco24I	GRGCYC	1 cut(s)	216
Eco31I	GGTCTC	3 cut(s)	349, 963, 1052
Eco47I	GGWCC	1 cut(s)	253
Eco53kI	GAGCTC	1 cut(s)	214
Eco57I	CTGAAG	2 cut(s)	587, 1188
Eco88I	CYCGRG	1 cut(s)	407
EcoICRI	GAGCTC	1 cut(s)	214
EcoO109I	RGGNCCY	1 cut(s)	253
EcoRII	CCWGG	1 cut(s)	654
EcoT22I	ATGCAT	1 cut(s)	1039
EcoT38I	GRGCYC	1 cut(s)	216
FaeI	CATG	3 cut(s)	910, 1133, 1313
FalI	AAGNNNNNCTT	2 cut(s)	517, 549
FatI	CATG	3 cut(s)	906, 1129, 1309
FauI	CCCGC	1 cut(s)	681
FbaI	TGATCA	1 cut(s)	33
FblI	GTMKAC	1 cut(s)	116
Fnu4HI	GCNGC	6 cut(s)	44, 333, 375, 964, 999, 1078
FokI	GGATG	1 cut(s)	632
FriOI	GRGCYC	1 cut(s)	216
Fsp4HI	GCNGC	6 cut(s)	44, 333, 375, 964, 999, 1078
FspBI	CTAG	1 cut(s)	642
GlaI	GCGC	1 cut(s)	1112
GluI	GCNGC	6 cut(s)	44, 333, 375, 964, 999, 1078
HaeIII	GGCC	1 cut(s)	335
HapII	CCGG	2 cut(s)	130, 684
HhaI	GCGC	1 cut(s)	1113
Hin1II	CATG	3 cut(s)	910, 1133, 1313
Hin6I	GCGC	1 cut(s)	1111
HinP1I	GCGC	1 cut(s)	1111
HincII	GTYRAC	2 cut(s)	59, 826
HindII	GTYRAC	2 cut(s)	59, 826
HindIII	AAGCTT	2 cut(s)	287, 1151
HinfI	GANTC	8 cut(s)	121, 326, 395, 428, 755, 898, 935, 1202
HpaII	CCGG	2 cut(s)	130, 684
HphI	GGTGA	4 cut(s)	136, 528, 673, 698
Hpy166II	GTNNAC	7 cut(s)	59, 117, 315, 826, 892, 1101, 1306
Hpy188I	TCNGA	4 cut(s)	94, 550, 1168, 1382
Hpy188III	TCNNGA	9 cut(s)	23, 154, 188, 409, 642, 902, 922, 1030, 1253
Hpy8I	GTNNAC	7 cut(s)	59, 117, 315, 826, 892, 1101, 1306
HpyAV	CCTTC	5 cut(s)	406, 562, 610, 919, 1271
HpyCH4III	ACNGT	5 cut(s)	205, 732, 1043, 1098, 1303
HpyCH4IV	ACGT	3 cut(s)	55, 628, 796
HpyF10VI	GCNNNNNNNGC	4 cut(s)	135, 295, 329, 431
HpyF3I	CTNAG	7 cut(s)	318, 370, 735, 800, 1174, 1221, 1379
HpySE526I	ACGT	3 cut(s)	55, 628, 796
Hsp92II	CATG	3 cut(s)	910, 1133, 1313
HspAI	GCGC	1 cut(s)	1111
Ksp22I	TGATCA	1 cut(s)	33
Kzo9I	GATC	2 cut(s)	33, 89
LguI	GCTCTTC	1 cut(s)	70
LmnI	GCTCC	1 cut(s)	211
Lsp1109I	GCAGC	5 cut(s)	55, 386, 975, 985, 1089
LweI	GCATC	3 cut(s)	762, 950, 1345
MaeI	CTAG	1 cut(s)	642
MaeII	ACGT	3 cut(s)	55, 628, 796
MaeIII	GTNAC	5 cut(s)	244, 624, 715, 1183, 1253
MalI	GATC	2 cut(s)	35, 91
MboI	GATC	2 cut(s)	33, 89
MfeI	CAATTG	1 cut(s)	885
MhlI	GDGCHC	2 cut(s)	216, 1103
MluCI	AATT	6 cut(s)	18, 191, 515, 885, 1005, 1343
MlyI	GAGTC	3 cut(s)	130, 404, 437
Mph1103I	ATGCAT	1 cut(s)	1039
MseI	TTAA	3 cut(s)	237, 792, 1417
MspI	CCGG	2 cut(s)	130, 684
MspR9I	CCNGG	1 cut(s)	656
MunI	CAATTG	1 cut(s)	885
Mva1269I	GAATGC	2 cut(s)	427, 1039
MvaI	CCWGG	1 cut(s)	656
MwoI	GCNNNNNNNGC	4 cut(s)	135, 295, 329, 431
NdeII	GATC	2 cut(s)	33, 89
NlaIII	CATG	3 cut(s)	910, 1133, 1313
NlaIV	GGNNCC	1 cut(s)	635
NmeAIII	GCCGAG	1 cut(s)	992
NmuCI	GTSAC	2 cut(s)	1183, 1253
NsiI	ATGCAT	1 cut(s)	1039
PciSI	GCTCTTC	1 cut(s)	70
PcsI	WCGNNNNNNNCGW	1 cut(s)	275
PctI	GAATGC	2 cut(s)	427, 1039
PfeI	GAWTC	5 cut(s)	326, 755, 898, 935, 1202
PfoI	TCCNGGA	1 cut(s)	654
PinAI	ACCGGT	1 cut(s)	683
PkrI	GCNGC	6 cut(s)	45, 334, 376, 965, 1000, 1079
PleI	GAGTC	3 cut(s)	129, 403, 436
PpsI	GAGTC	3 cut(s)	129, 403, 436
Ppu21I	YACGTR	1 cut(s)	629
PpuMI	RGGWCCY	1 cut(s)	253
Psp124BI	GAGCTC	1 cut(s)	216
Psp5II	RGGWCCY	1 cut(s)	253
Psp6I	CCWGG	1 cut(s)	654
PspGI	CCWGG	1 cut(s)	654
PspN4I	GGNNCC	1 cut(s)	635
PspPI	GGNCC	1 cut(s)	253
PspPPI	RGGWCCY	1 cut(s)	253
PstI	CTGCAG	1 cut(s)	1003
PstNI	CAGNNNCTG	2 cut(s)	938, 1181
RsaI	GTAC	1 cut(s)	1307
RsaNI	GTAC	1 cut(s)	1306
SacI	GAGCTC	1 cut(s)	216
SapI	GCTCTTC	1 cut(s)	70
SaqAI	TTAA	3 cut(s)	237, 792, 1417
SatI	GCNGC	6 cut(s)	44, 333, 375, 964, 999, 1078
Sau3AI	GATC	2 cut(s)	33, 89
Sau96I	GGNCC	1 cut(s)	253
SchI	GAGTC	3 cut(s)	130, 404, 437
ScrFI	CCNGG	1 cut(s)	656
SduI	GDGCHC	2 cut(s)	216, 1103
SfaNI	GCATC	3 cut(s)	762, 950, 1345
SfcI	CTRYAG	1 cut(s)	999
SgrAI	CRCCGGYG	1 cut(s)	683
SinI	GGWCC	1 cut(s)	253
SmlI	CTYRAG	2 cut(s)	215, 459
SmoI	CTYRAG	2 cut(s)	215, 459
Sse9I	AATT	6 cut(s)	18, 191, 515, 885, 1005, 1343
SsiI	CCGC	3 cut(s)	332, 674, 804
SspI	AATATT	1 cut(s)	163
SspMI	CTAG	1 cut(s)	642
SstI	GAGCTC	1 cut(s)	216
StyD4I	CCNGG	1 cut(s)	654
TaaI	ACNGT	5 cut(s)	205, 732, 1043, 1098, 1303
TaiI	ACGT	3 cut(s)	58, 631, 799
TaqI	TCGA	3 cut(s)	22, 155, 1143
TasI	AATT	6 cut(s)	18, 191, 515, 885, 1005, 1343
TauI	GCSGC	1 cut(s)	335
TfiI	GAWTC	5 cut(s)	326, 755, 898, 935, 1202
Tru1I	TTAA	3 cut(s)	237, 792, 1417
Tru9I	TTAA	3 cut(s)	237, 792, 1417
TscAI	CASTG	3 cut(s)	385, 880, 1048
TseFI	GTSAC	2 cut(s)	1183, 1253
TseI	GCWGC	5 cut(s)	43, 374, 963, 998, 1077
Tsp45I	GTSAC	2 cut(s)	1183, 1253
TspDTI	ATGAA	3 cut(s)	429, 923, 1326
TspGWI	ACGGA	1 cut(s)	503
TspRI	CASTG	3 cut(s)	385, 880, 1048
VneI	GTGCAC	1 cut(s)	1099
VpaK11BI	GGWCC	1 cut(s)	253
XapI	RAATTY	2 cut(s)	191, 515
XbaI	TCTAGA	1 cut(s)	641
XmiI	GTMKAC	1 cut(s)	116
XspI	CTAG	1 cut(s)	642
Zsp2I	ATGCAT	1 cut(s)	1039

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.