Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0002467

Physical Location & Seq

Genomic Coordinates Forward (+)

32442097 .. 32446386

Gene Structure 4290 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1197100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1467 bp

ATGGCGGGCAGAAAGCTAGTGCGAGATTTGCACCTCTACAGACAACCCCTCTTTCTTCATCTGACCTCCCAACAGCAGGTTTCGGGCACGAGAGGTCGATTGATTTCGGCCAATGGGTTTTTGGGGAATCGTCGATTCAGTGTCTTCAATGAGTTTTCGAAGCAAGTCAAAGGAGAAGCTACTAAGAATCCCGAATTTCAACAGTCAGTGAAGGAGCTGAAAGAGAAAGCGGAAGAGCTCAAAGGGGTTAAAGAAGATTTGAAAGTGAGAACGAAGCAGACAAGTGAGCAGCTATACAAGCGGGTGGATGGTGCGTGGACAGAGGCGGAAGCAACGGCAAAGAAGGTTTCTGCCAATGTAAAGGAGAAGATCTCGGCTGCCACAGAGGAGGTCAAAGGAACTTTTGGAATTGAGAAAGAAGAGGCTTCAGGGCCTTCTGGTGCAAAATCTGAGCATGTTGCTGATGATGGAATGAAGGGCTCGTCTGGGGAACATAATCACCAGCAATCTGGGGAATATAAAAACCAGCAGTCAGGGTCTAGTAGTACTGAACAAACATTTTTTGGAAAATTTAGGTCAAGTGTTTCTTCCTCGTATTTTTCCTCTGCCTTCTCTAGAGTTAAGGATGCAAAGGTTACGGACTTGGCTAAAAAAGGATATGACATTGTAAAGGGTGAGTTAACTGGTACTACAACTAAGAGAAAGCACCTGGAGTTTGATCCTTCTTCGACCCCGAAAGTTGAAACTAGTTCAAGAACGGACCTTGTTGCTGTACCCCAATCTCGCTGGAGTAAAAAGTGGGAGGCTTTCAGAATGAAGATGCAAGGTTATCCTATATTCAAGCGTCTTGATGAAATACGTAAACCAGTTACAAACAAAACCCAGGAGATTGCTGAGGACATACGGGATCGTTGGGATACAAGCGATAGCCCAATCGTTCACAAAATTCAGGATTTAAACGAAACTATTTTTCAAGAAACAGACACCGCTGCATCAATCAAGGAGATACGTCGCCGAGATCCATCCTTCTCTTTACCAGACTTTTTGTCAGAAGTTCAGGATGCTGTTAAACCTGTGCTGAATGCTTACATCAAGGGGGATCTTGAGACATTGAAGAAGTATTGTAGTAAGGAGGTGATTGAGCGGTGTAAAGCTGAGCATGATGGTTTTAAAAGCCATGGCATCTTTTTCGATCACAAGATTCTACATATTTCTGAGGCGGAAATAAAAGAGACCAAAATGATGGGACATTCTCCCATTATTATTGTAATGTTCCGAACGCAGCAAGTGTATTGTGTACGAGATAGAAATGGTGATGTAACTGATGGTGGCAAGGACACTATCCACACGGTGTATTATGCCTGGGCAATGCAACAGGTAGATCCAGAAGAACTCGGTGAAGGTGCTATTTATCCAATTTGGAAAGTAAGAGAGATGCAGCAATTCGGAGTTCAAGCCCTCATTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0005744 GO:0005759 GO:0006605 GO:0006626 GO:0006810 GO:0006839 GO:0006886 GO:0006996 GO:0007005 GO:0008104 GO:0008150 GO:0008320 GO:0008565 GO:0009987 GO:0015031 GO:0015399 GO:0015405 GO:0015440 GO:0015450 GO:0015462 GO:0015833 GO:0016020 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017038 GO:0017111 GO:0019866 GO:0022804 GO:0022857 GO:0022884 GO:0030150 GO:0031090 GO:0031966 GO:0031967 GO:0031974 GO:0031975 GO:0032991 GO:0033036 GO:0033220 GO:0033365 GO:0034613 GO:0042623 GO:0042626 GO:0042886 GO:0042887 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0044743 GO:0045184 GO:0046907 GO:0051087 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055085 GO:0065002 GO:0070013 GO:0070585 GO:0070727 GO:0071702 GO:0071705 GO:0071806 GO:0071840 GO:0072594 GO:0072655 GO:0098796 GO:0098798 GO:0098800 GO:1904680 GO:1990542

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Tim44

Protein Analysis

489

Amino Acids

55.31

Weight (kDa)

8.82

Isoelectric Point (pI)

41.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Tim44	PF04280	330 - 481	1.9e-42	Tim44-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002467)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G20510 AT2G20510 AT2G20510 AT2G20510 AT2G36070
fragaria_vesca	FvH4_2g21470 FvH4_2g21470 FvH4_4g12040 FvH4_4g12040 FvH4_4g12040 FvH4_4g12040
malus_domestica	MD05G1197100.v1.1 MD09G1008600.v1.1
prunus_persica	Prupe.8G223600_v2.0.a1
pyrus_communis	pycom05g18420 pycom10g15910
rosa_chinensis	RchiOBHm_Chr4g0409381 RchiOBHm_Chr5g0013581 RchiOBHm_Chr6g0288111
rosa_laevigata	RLG00000012438 RLG00000032032
rosa_multiflora	Rmu_co8365065.1_g000001 Rmu_sc0001616.1_g000049 Rmu_sc0001851.1_g000030 Rmu_sc0010766.1_g000003
rosa_roxburghii	Rroxscaffold_1G00062990 Rroxscaffold_5G00353910 Rroxscaffold_7G00180510
rosa_rugosa	Rorug04G0093400 Rorug06G0194200
rosa_samantha	Rh4AG155900 Rh4BG155100 Rh4CG165200 Rh4DG149000 Rh5BG102300 Rh6AG304400 Rh6BG310400 Rh6CG318500 Rh6DG303400
rosa_wichuraiana	Rw4G012760 Rw6G026340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	67
AccBSI	CCGCTC	1 cut(s)	1144
AciI	CCGC	7 cut(s)	5, 230, 301, 326, 987, 1144, 1220
AclWI	GGATC	5 cut(s)	713, 915, 1013, 1107, 1376
AcoI	YGGCCR	1 cut(s)	108
AcsI	RAATTY	3 cut(s)	194, 569, 945
AcuI	CTGAAG	1 cut(s)	411
AdeI	CACNNNGTG	1 cut(s)	1351
AfaI	GTAC	4 cut(s)	547, 688, 774, 1299
AfiI	CCNNNNNNNGG	1 cut(s)	76
AgsI	TTSAA	9 cut(s)	148, 200, 262, 743, 753, 841, 974, 1114, 1454
AhdI	GACNNNNNGTC	1 cut(s)	1045
AhlI	ACTAGT	1 cut(s)	746
AjnI	CCWGG	3 cut(s)	708, 882, 1361
AluBI	AGCT	6 cut(s)	16, 179, 217, 238, 292, 1154
AluI	AGCT	6 cut(s)	16, 179, 217, 238, 292, 1154
Alw21I	GWGCWC	1 cut(s)	240
Alw26I	GTCTC	2 cut(s)	1100, 1226
AlwI	GGATC	5 cut(s)	713, 915, 1013, 1107, 1376
AoxI	GGCC	2 cut(s)	108, 431
ApeKI	GCWGC	5 cut(s)	289, 377, 989, 1282, 1438
ApoI	RAATTY	3 cut(s)	194, 569, 945
AspS9I	GGNCC	2 cut(s)	431, 760
AsuHPI	GGTGA	5 cut(s)	491, 686, 1147, 1325, 1409
AsuII	TTCGAA	1 cut(s)	158
AvaII	GGWCC	1 cut(s)	760
BaeGI	GKGCMC	1 cut(s)	89
BanII	GRGCYC	2 cut(s)	240, 482
BauI	CACGAG	1 cut(s)	88
BbsI	GAAGAC	1 cut(s)	136
Bbv12I	GWGCWC	1 cut(s)	240
BbvCI	CCTCAGC	1 cut(s)	894
BbvI	GCAGC	5 cut(s)	301, 364, 976, 1294, 1450
BccI	CCATC	6 cut(s)	302, 461, 1030, 1157, 1237, 1319
BceAI	ACGGC	1 cut(s)	351
BcgI	CGANNNNNNTGC	2 cut(s)	1171, 1205
BciT130I	CCWGG	3 cut(s)	710, 884, 1363
BciVI	GTATCC	1 cut(s)	910
BcoDI	GTCTC	2 cut(s)	1100, 1226
BcuI	ACTAGT	1 cut(s)	746
BfaI	CTAG	4 cut(s)	17, 540, 615, 747
BfmI	CTRYAG	1 cut(s)	37
BfuAI	ACCTGC	1 cut(s)	67
BfuI	GTATCC	1 cut(s)	910
BglII	AGATCT	1 cut(s)	369
BisI	GCNGC	5 cut(s)	290, 378, 990, 1283, 1439
BlpI	GCTNAGC	1 cut(s)	1155
BlsI	GCNGC	5 cut(s)	291, 379, 991, 1284, 1440
BmcAI	AGTACT	1 cut(s)	547
Bme1390I	CCNGG	3 cut(s)	710, 884, 1363
Bme18I	GGWCC	1 cut(s)	760
BmeRI	GACNNNNNGTC	1 cut(s)	1045
BmgT120I	GGNCC	2 cut(s)	431, 760
BmrFI	CCNGG	3 cut(s)	710, 884, 1363
BmsI	GCATC	6 cut(s)	616, 810, 1001, 1051, 1191, 1425
BpiI	GAAGAC	1 cut(s)	136
BplI	GAGNNNNNCTC	2 cut(s)	356, 388
BpmI	CTGGAG	2 cut(s)	731, 808
Bpu10I	CCTNAGC	1 cut(s)	894
Bpu1102I	GCTNAGC	1 cut(s)	1155
Bpu14I	TTCGAA	1 cut(s)	158
BpuEI	CTTGAG	1 cut(s)	1124
BsaAI	YACGTR	1 cut(s)	860
BsaI	GGTCTC	1 cut(s)	1226
BsaJI	CCNNGG	3 cut(s)	882, 1177, 1362
Bsc4I	CCNNNNNNNGG	1 cut(s)	76
Bse1I	ACTGG	2 cut(s)	688, 866
Bse3DI	GCAATG	1 cut(s)	1374
BseBI	CCWGG	3 cut(s)	710, 884, 1363
BseDI	CCNNGG	3 cut(s)	882, 1177, 1362
BseGI	GGATG	4 cut(s)	313, 631, 1022, 1066
BseLI	CCNNNNNNNGG	1 cut(s)	76
BseMI	GCAATG	1 cut(s)	1374
BseMII	CTCAG	4 cut(s)	441, 885, 1146, 1206
BseNI	ACTGG	2 cut(s)	688, 866
BseRI	GAGGAG	1 cut(s)	401
BseSI	GKGCMC	1 cut(s)	89
BseXI	GCAGC	5 cut(s)	301, 364, 976, 1294, 1450
BshFI	GGCC	2 cut(s)	110, 433
BsiHKAI	GWGCWC	1 cut(s)	240
BslFI	GGGAC	1 cut(s)	1260
BslI	CCNNNNNNNGG	1 cut(s)	76
BsmAI	GTCTC	2 cut(s)	1100, 1226
BsmFI	GGGAC	1 cut(s)	1260
BsmI	GAATGC	1 cut(s)	1087
BsnI	GGCC	2 cut(s)	110, 433
Bso31I	GGTCTC	1 cut(s)	1226
Bsp119I	TTCGAA	1 cut(s)	158
Bsp1286I	GDGCHC	3 cut(s)	89, 240, 482
Bsp143I	GATC	7 cut(s)	369, 718, 907, 1018, 1099, 1192, 1381
Bsp1720I	GCTNAGC	1 cut(s)	1155
Bsp19I	CCATGG	1 cut(s)	1177
BspACI	CCGC	7 cut(s)	5, 230, 301, 326, 987, 1144, 1220
BspANI	GGCC	2 cut(s)	110, 433
BspCNI	CTCAG	4 cut(s)	442, 886, 1147, 1207
BspMI	ACCTGC	1 cut(s)	67
BspPI	GGATC	5 cut(s)	713, 915, 1013, 1107, 1376
BspQI	GCTCTTC	1 cut(s)	228
BspT104I	TTCGAA	1 cut(s)	158
BspTNI	GGTCTC	1 cut(s)	1226
BsrBI	CCGCTC	1 cut(s)	1144
BsrDI	GCAATG	1 cut(s)	1374
BsrI	ACTGG	2 cut(s)	688, 866
BssECI	CCNNGG	3 cut(s)	882, 1177, 1362
BssMI	GATC	7 cut(s)	369, 718, 907, 1018, 1099, 1192, 1381
BssSI	CACGAG	1 cut(s)	88
BssT1I	CCWWGG	1 cut(s)	1177
Bst2BI	CACGAG	1 cut(s)	88
Bst2UI	CCWGG	3 cut(s)	710, 884, 1363
Bst4CI	ACNGT	2 cut(s)	204, 1351
Bst6I	CTCTTC	2 cut(s)	228, 414
BstBAI	YACGTR	1 cut(s)	860
BstBI	TTCGAA	1 cut(s)	158
BstC8I	GCNNGC	1 cut(s)	7
BstDEI	CTNAG	6 cut(s)	183, 450, 696, 894, 1155, 1215
BstDSI	CCRYGG	1 cut(s)	1177
BstF5I	GGATG	4 cut(s)	313, 631, 1022, 1066
BstKTI	GATC	7 cut(s)	372, 721, 910, 1021, 1102, 1195, 1384
BstMAI	GTCTC	2 cut(s)	1100, 1226
BstMBI	GATC	7 cut(s)	369, 718, 907, 1018, 1099, 1192, 1381
BstMWI	GCNNNNNNNGC	2 cut(s)	28, 298
BstNI	CCWGG	3 cut(s)	710, 884, 1363
BstNSI	RCATGY	1 cut(s)	458
BstSCI	CCNGG	3 cut(s)	708, 882, 1361
BstSFI	CTRYAG	1 cut(s)	37
BstSLI	GKGCMC	1 cut(s)	89
BstSNI	TACGTA	1 cut(s)	860
BstV1I	GCAGC	5 cut(s)	301, 364, 976, 1294, 1450
BstV2I	GAAGAC	1 cut(s)	136
BstX2I	RGATCY	4 cut(s)	369, 1018, 1099, 1381
BstXI	CCANNNNNNTGG	2 cut(s)	509, 1243
BstYI	RGATCY	4 cut(s)	369, 1018, 1099, 1381
BsuI	GTATCC	1 cut(s)	910
BsuRI	GGCC	2 cut(s)	110, 433
BtgI	CCRYGG	1 cut(s)	1177
BtsCI	GGATG	4 cut(s)	313, 631, 1022, 1066
BtsIMutI	CAGTG	2 cut(s)	145, 213
BveI	ACCTGC	1 cut(s)	67
Cac8I	GCNNGC	1 cut(s)	7
Cfr13I	GGNCC	2 cut(s)	431, 760
CseI	GACGC	1 cut(s)	833
Csp6I	GTAC	4 cut(s)	546, 687, 773, 1298
CviAII	CATG	3 cut(s)	455, 1160, 1178
CviQI	GTAC	4 cut(s)	546, 687, 773, 1298
DdeI	CTNAG	6 cut(s)	183, 450, 696, 894, 1155, 1215
DpnI	GATC	7 cut(s)	371, 720, 909, 1020, 1101, 1194, 1383
DpnII	GATC	7 cut(s)	369, 718, 907, 1018, 1099, 1192, 1381
DraI	TTTAAA	2 cut(s)	957, 1171
DraIII	CACNNNGTG	1 cut(s)	1351
DriI	GACNNNNNGTC	1 cut(s)	1045
EaeI	YGGCCR	1 cut(s)	108
Eam1104I	CTCTTC	2 cut(s)	228, 414
Eam1105I	GACNNNNNGTC	1 cut(s)	1045
EarI	CTCTTC	2 cut(s)	228, 414
EciI	GGCGGA	2 cut(s)	341, 1235
Ecl136II	GAGCTC	1 cut(s)	238
Eco105I	TACGTA	1 cut(s)	860
Eco130I	CCWWGG	1 cut(s)	1177
Eco24I	GRGCYC	2 cut(s)	240, 482
Eco31I	GGTCTC	1 cut(s)	1226
Eco47I	GGWCC	1 cut(s)	760
Eco53kI	GAGCTC	1 cut(s)	238
Eco57I	CTGAAG	1 cut(s)	411
EcoICRI	GAGCTC	1 cut(s)	238
EcoO109I	RGGNCCY	1 cut(s)	431
EcoRII	CCWGG	3 cut(s)	708, 882, 1361
EcoT14I	CCWWGG	1 cut(s)	1177
EcoT38I	GRGCYC	2 cut(s)	240, 482
ErhI	CCWWGG	1 cut(s)	1177
FaeI	CATG	3 cut(s)	458, 1163, 1181
FalI	AAGNNNNNCTT	2 cut(s)	571, 603
FaqI	GGGAC	1 cut(s)	1260
FatI	CATG	3 cut(s)	454, 1159, 1177
FauI	CCCGC	1 cut(s)	294
Fnu4HI	GCNGC	5 cut(s)	290, 378, 990, 1283, 1439
FokI	GGATG	4 cut(s)	320, 638, 1009, 1073
FriOI	GRGCYC	2 cut(s)	240, 482
Fsp4HI	GCNGC	5 cut(s)	290, 378, 990, 1283, 1439
FspBI	CTAG	4 cut(s)	17, 540, 615, 747
GluI	GCNGC	5 cut(s)	290, 378, 990, 1283, 1439
GsuI	CTGGAG	2 cut(s)	731, 808
HaeIII	GGCC	2 cut(s)	110, 433
HgaI	GACGC	1 cut(s)	833
Hin1II	CATG	3 cut(s)	458, 1163, 1181
HincII	GTYRAC	1 cut(s)	681
HindII	GTYRAC	1 cut(s)	681
HinfI	GANTC	4 cut(s)	127, 135, 187, 1201
HpaI	GTTAAC	1 cut(s)	681
HphI	GGTGA	5 cut(s)	491, 686, 1147, 1325, 1409
Hpy166II	GTNNAC	5 cut(s)	318, 681, 863, 940, 1298
Hpy188I	TCNGA	7 cut(s)	63, 451, 812, 1051, 1216, 1277, 1448
Hpy188III	TCNNGA	9 cut(s)	191, 615, 753, 848, 950, 974, 1058, 1103, 1385
Hpy8I	GTNNAC	5 cut(s)	318, 681, 863, 940, 1298
Hpy99I	CGWCG	2 cut(s)	135, 1014
HpyAV	CCTTC	8 cut(s)	205, 337, 444, 469, 619, 732, 1036, 1394
HpyCH4III	ACNGT	2 cut(s)	204, 1351
HpyCH4IV	ACGT	2 cut(s)	859, 1009
HpyCH4V	TGCA	7 cut(s)	31, 443, 629, 823, 992, 1372, 1438
HpyF10VI	GCNNNNNNNGC	2 cut(s)	28, 298
HpyF3I	CTNAG	6 cut(s)	183, 450, 696, 894, 1155, 1215
HpySE526I	ACGT	2 cut(s)	859, 1009
Hsp92II	CATG	3 cut(s)	458, 1163, 1181
KspAI	GTTAAC	1 cut(s)	681
Kzo9I	GATC	7 cut(s)	369, 718, 907, 1018, 1099, 1192, 1381
LguI	GCTCTTC	1 cut(s)	228
LmnI	GCTCC	1 cut(s)	214
Lsp1109I	GCAGC	5 cut(s)	301, 364, 976, 1294, 1450
LweI	GCATC	6 cut(s)	616, 810, 1001, 1051, 1191, 1425
MaeI	CTAG	4 cut(s)	17, 540, 615, 747
MaeII	ACGT	2 cut(s)	859, 1009
MaeIII	GTNAC	3 cut(s)	634, 868, 1318
MalI	GATC	7 cut(s)	371, 720, 909, 1020, 1101, 1194, 1383
MbiI	CCGCTC	1 cut(s)	1144
MboI	GATC	7 cut(s)	369, 718, 907, 1018, 1099, 1192, 1381
MflI	RGATCY	4 cut(s)	369, 1018, 1099, 1381
MhlI	GDGCHC	3 cut(s)	89, 240, 482
MluCI	AATT	6 cut(s)	194, 408, 569, 945, 1416, 1442
MseI	TTAA	6 cut(s)	249, 621, 680, 956, 1068, 1170
MspA1I	CMGCKG	1 cut(s)	989
MspR9I	CCNGG	3 cut(s)	710, 884, 1363
Mva1269I	GAATGC	1 cut(s)	1087
MvaI	CCWGG	3 cut(s)	710, 884, 1363
MwoI	GCNNNNNNNGC	2 cut(s)	28, 298
NcoI	CCATGG	1 cut(s)	1177
NdeII	GATC	7 cut(s)	369, 718, 907, 1018, 1099, 1192, 1381
NlaIII	CATG	3 cut(s)	458, 1163, 1181
NmeAIII	GCCGAG	2 cut(s)	353, 1040
NspI	RCATGY	1 cut(s)	458
NspV	TTCGAA	1 cut(s)	158
PciSI	GCTCTTC	1 cut(s)	228
PctI	GAATGC	1 cut(s)	1087
PfeI	GAWTC	4 cut(s)	127, 135, 187, 1201
PkrI	GCNGC	5 cut(s)	291, 379, 991, 1284, 1440
Ppu21I	YACGTR	1 cut(s)	860
Psp124BI	GAGCTC	1 cut(s)	240
Psp6I	CCWGG	3 cut(s)	708, 882, 1361
PspGI	CCWGG	3 cut(s)	708, 882, 1361
PspPI	GGNCC	2 cut(s)	431, 760
PsuI	RGATCY	4 cut(s)	369, 1018, 1099, 1381
RsaI	GTAC	4 cut(s)	547, 688, 774, 1299
RsaNI	GTAC	4 cut(s)	546, 687, 773, 1298
SacI	GAGCTC	1 cut(s)	240
SapI	GCTCTTC	1 cut(s)	228
SaqAI	TTAA	6 cut(s)	249, 621, 680, 956, 1068, 1170
SatI	GCNGC	5 cut(s)	290, 378, 990, 1283, 1439
Sau3AI	GATC	7 cut(s)	369, 718, 907, 1018, 1099, 1192, 1381
Sau96I	GGNCC	2 cut(s)	431, 760
ScaI	AGTACT	1 cut(s)	547
ScrFI	CCNGG	3 cut(s)	710, 884, 1363
SduI	GDGCHC	3 cut(s)	89, 240, 482
SfaNI	GCATC	6 cut(s)	616, 810, 1001, 1051, 1191, 1425
SfcI	CTRYAG	1 cut(s)	37
SfuI	TTCGAA	1 cut(s)	158
SinI	GGWCC	1 cut(s)	760
SmlI	CTYRAG	1 cut(s)	1103
SmoI	CTYRAG	1 cut(s)	1103
SnaBI	TACGTA	1 cut(s)	860
SpeI	ACTAGT	1 cut(s)	746
Sse9I	AATT	6 cut(s)	194, 408, 569, 945, 1416, 1442
SsiI	CCGC	7 cut(s)	5, 230, 301, 326, 987, 1144, 1220
SspMI	CTAG	4 cut(s)	17, 540, 615, 747
SstI	GAGCTC	1 cut(s)	240
StyD4I	CCNGG	3 cut(s)	708, 882, 1361
StyI	CCWWGG	1 cut(s)	1177
TaaI	ACNGT	2 cut(s)	204, 1351
TaiI	ACGT	2 cut(s)	862, 1012
TaqI	TCGA	5 cut(s)	97, 133, 158, 728, 1191
TasI	AATT	6 cut(s)	194, 408, 569, 945, 1416, 1442
TatI	WGTACW	1 cut(s)	545
TfiI	GAWTC	4 cut(s)	127, 135, 187, 1201
Tru1I	TTAA	6 cut(s)	249, 621, 680, 956, 1068, 1170
Tru9I	TTAA	6 cut(s)	249, 621, 680, 956, 1068, 1170
TscAI	CASTG	2 cut(s)	145, 213
TseI	GCWGC	5 cut(s)	289, 377, 989, 1282, 1438
TspDTI	ATGAA	4 cut(s)	47, 488, 830, 867
TspGWI	ACGGA	2 cut(s)	653, 773
TspRI	CASTG	2 cut(s)	145, 213
VpaK11BI	GGWCC	1 cut(s)	760
XapI	RAATTY	3 cut(s)	194, 569, 945
XbaI	TCTAGA	1 cut(s)	614
XceI	RCATGY	1 cut(s)	458
XcmI	CCANNNNNNNNNTGG	1 cut(s)	118
XspI	CTAG	4 cut(s)	17, 540, 615, 747
ZrmI	AGTACT	1 cut(s)	547

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.