AT2G22480

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0008632
Physical Location & Seq
Forward (+)
9545283 .. 9548640
3358 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G22480.1

Sequence Viewer

Length: 1614 bp
ATGGATGCTCTTTCTCAGGCGATCAGTTCCGGGATCTCCGTTCCTTACAAGAACAATTCTTCTTCTCTCGTTCCTTCTCACGGACTCACCTCCTTAATCCTCCGGAAATCGAGATCTCCGGTGAATCCCTCATCACGATCTCGCGTCTCGGTGCGAGCTTCGGAGATTCAACACAGCAAAACCTCAGCTTCATCGATCGATCTCAGCGATCCAGATTGGAAATTAAAGTATGAGAAAGATTTCGAGCAACGATTCAGCATACCTCACATCACTGATGTCTTACCTGATGCTGAAGCCATTCGTTCAACGTTTTGTCTTAAGATGAGGTCTCCGACGGAAGATTTTGTTGGTGGTTACCCTTCTGATGAAGAATGGCATGGATACATTAATAACAATGATAGAGTTCTTCTCAAGGTTATTAGTTACTCCTCACCTACTTCTGCTGGAGCTGAGTGCCTTGATCATGACTGTTCTTGGGTTGAACAATGGATTCACCGTGCTGGTCCGAGGGAGAAGATATACTTCAGGCCGGAAGAAGTGAAAGCTGCGATTATCACTTGTGGTGGCCTTTGCCCTGGTCTCAATGATGTCATCAGACATATTGTCATTACTCTTGAGATTTATGGTGTTAAGAACATTGTGGGGATTCCTTTCGGTTATCGAGGCTTCTCTGATAAAGATCTAACTGAAATGCCGTTATCAAGGAAAGTGGTTCAGAATATTCATTTATCTGGAGGAAGTTTGCTTGGAGTTTCACGTGGAGGCCCGAGTGTGAGTGAAATTGTCGACAGCATGGAGGAGAGAGGAATCAACATGCTTTTCGTGCTCGGTGGAAACGGAACTCATGCTGGCGCCAACGCTATACACAATGAGTGCCGCAAAAGAAAGATAAAGGTAGCTGTAGTTGGTGTGCCAAAAACCATCGACAATGATATTTTACATATGGATAAAACTTTTGGGTTTGATACTGCTGTTGAAGAAGCTCAACGAGCAATTAACTCTGCTTACATTGAGGCACATAGTGCTTATCATGGCATTGGCGTTGTAAAACTGATGGGTCGTAACAGTGGTTTCATTGCTATGCAAGCCTCTCTAGCAAGTGGACAAGTCGACATCTGTTTGATTCCTGAGGTTCCCTTCAATCTTCATGGGCCTAATGGTGTATTGAAGCATTTGAAGTACCTTATTGAAACAAAAGGCTCTGCTGTGATCTGTGTAGCAGAAGGAGCTGGACAGAATTTTCTTGAGAAAACCAATGCCAAAGATGCATCTGGAAACGCCGTACTTGGTGATTTCGGTGTGTATATTCAACAAGAGACTAAGAAGTATTTCAAAGAAATAAGTACTCCAATAGATGTGAAGTATATTGATCCAACATACATGATTCGCGCTGTACGTGCAAATGCCTCGGATGGTATCCTCTGCACCGTTCTTGGACAAAACGCTGTTCATGGTGCGTTTGCTGGATACAGTGGAATCACGGTAGGCATAATCAACACTCACTATGCATATTTGCCAATCACTGAGGTAATTGCATATCCAAAATCAGTGGATCCTAATAGTCGAATGTGGCATCGTTGCTTGACATCAACGGGCCAACCCGATTTCATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003872 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008443 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009536 GO:0009987 GO:0016052 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019200 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044262 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046835 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0061615 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko00051 ko00052 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04152 ko05230 map00010 map00030 map00051 map00052 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04152 map05230
Pfam Domains
Protein Families
PFK

Protein Analysis

537

Amino Acids

58.61

Weight (kDa)

6.81

Isoelectric Point (pI)

39.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PFK PF00365 182 - 484 3.2e-63 Phosphofructokinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 851
AccI GTMKAC 2 cut(s) 786, 1110
AccII CGCG 2 cut(s) 144, 1389
AccIII TCCGGA 1 cut(s) 102
AciI CCGC 1 cut(s) 877
AclI AACGTT 1 cut(s) 308
AclWI GGATC 5 cut(s) 41, 203, 1364, 1547, 1560
AcsI RAATTY 1 cut(s) 1237
AcuI CTGAAG 2 cut(s) 312, 508
AcvI CACGTG 1 cut(s) 758
AcyI GRCGYC 1 cut(s) 852
AdeI CACNNNGTG 1 cut(s) 1022
AfaI GTAC 4 cut(s) 1181, 1284, 1345, 1395
AfiI CCNNNNNNNGG 1 cut(s) 80
AflII CTTAAG 1 cut(s) 317
AhdI GACNNNNNGTC 1 cut(s) 602
AjnI CCWGG 1 cut(s) 574
AjuI GAANNNNNNNTTGG 2 cut(s) 330, 362
AluBI AGCT 7 cut(s) 158, 188, 449, 545, 899, 983, 1229
AluI AGCT 7 cut(s) 158, 188, 449, 545, 899, 983, 1229
Alw21I GWGCWC 1 cut(s) 828
Alw26I GTCTC 4 cut(s) 151, 333, 584, 1310
AlwI GGATC 5 cut(s) 41, 203, 1364, 1547, 1560
Ama87I CYCGRG 1 cut(s) 766
Aor13HI TCCGGA 1 cut(s) 102
AoxI GGCC 5 cut(s) 527, 565, 763, 1151, 1594
ApeKI GCWGC 1 cut(s) 545
ApoI RAATTY 1 cut(s) 1237
AseI ATTAAT 1 cut(s) 387
Asp700I GAANNNNTTC 3 cut(s) 239, 297, 1328
AspLEI GCGC 2 cut(s) 854, 1391
AspS9I GGNCC 4 cut(s) 503, 764, 1151, 1594
AsuC2I CCSGG 1 cut(s) 31
AsuHPI GGTGA 5 cut(s) 79, 133, 423, 485, 1301
AvaI CYCGRG 1 cut(s) 766
AvaII GGWCC 1 cut(s) 503
AxyI CCTNAGG 1 cut(s) 1128
BaeI ACNNNNGTAYC 2 cut(s) 957, 990
BamHI GGATCC 1 cut(s) 1552
BanI GGYRCC 1 cut(s) 851
BbrPI CACGTG 1 cut(s) 758
Bbv12I GWGCWC 1 cut(s) 828
BbvCI CCTCAGC 1 cut(s) 184
BbvI GCAGC 1 cut(s) 532
BccI CCATC 3 cut(s) 929, 1048, 1406
BceAI ACGGC 2 cut(s) 679, 1265
BcgI CGANNNNNNTGC 2 cut(s) 1389, 1423
BciT130I CCWGG 1 cut(s) 576
BciVI GTATCC 3 cut(s) 374, 1427, 1460
BclI TGATCA 1 cut(s) 460
BcnI CCSGG 1 cut(s) 31
BcoDI GTCTC 4 cut(s) 151, 333, 584, 1310
BfaI CTAG 1 cut(s) 1094
BfmI CTRYAG 1 cut(s) 900
BfoI RGCGCY 1 cut(s) 855
BfrI CTTAAG 1 cut(s) 317
BfuI GTATCC 3 cut(s) 374, 1427, 1460
BglII AGATCT 2 cut(s) 113, 679
BisI GCNGC 2 cut(s) 546, 877
BlsI GCNGC 2 cut(s) 547, 878
BmcAI AGTACT 1 cut(s) 1345
Bme1390I CCNGG 2 cut(s) 31, 576
Bme18I GGWCC 1 cut(s) 503
BmeRI GACNNNNNGTC 1 cut(s) 602
BmeT110I CYCGRG 1 cut(s) 766
BmgT120I GGNCC 4 cut(s) 503, 764, 1151, 1594
BmiI GGNNCC 3 cut(s) 853, 1134, 1554
BmrFI CCNGG 2 cut(s) 31, 576
BmsI GCATC 4 cut(s) 277, 1255, 1277, 1582
BplI GAGNNNNNCTC 2 cut(s) 393, 425
BpmI CTGGAG 2 cut(s) 465, 753
Bpu10I CCTNAGC 1 cut(s) 184
BpuEI CTTGAG 3 cut(s) 395, 635, 1265
BpuMI CCSGG 1 cut(s) 31
Bsa29I ATCGAT 2 cut(s) 194, 198
BsaAI YACGTR 2 cut(s) 758, 1397
BsaBI GATNNNNATC 1 cut(s) 678
BsaHI GRCGYC 1 cut(s) 852
BsaI GGTCTC 2 cut(s) 333, 584
BsaJI CCNNGG 3 cut(s) 506, 574, 1407
BsaWI WCCGGW 2 cut(s) 102, 118
Bsc4I CCNNNNNNNGG 1 cut(s) 80
Bse21I CCTNAGG 1 cut(s) 1128
Bse3DI GCAATG 1 cut(s) 1074
Bse8I GATNNNNATC 1 cut(s) 678
BseAI TCCGGA 1 cut(s) 102
BseBI CCWGG 1 cut(s) 576
BseCI ATCGAT 2 cut(s) 194, 198
BseDI CCNNGG 3 cut(s) 506, 574, 1407
BseGI GGATG 2 cut(s) 10, 1417
BseJI GATNNNNATC 1 cut(s) 678
BseLI CCNNNNNNNGG 1 cut(s) 80
BseMI GCAATG 1 cut(s) 1074
BseMII CTCAG 6 cut(s) 29, 198, 217, 441, 1119, 1515
BseRI GAGGAG 2 cut(s) 418, 812
BseXI GCAGC 1 cut(s) 532
BsgI GTGCAG 1 cut(s) 1408
Bsh1236I CGCG 2 cut(s) 144, 1389
Bsh1285I CGRYCG 1 cut(s) 198
BshFI GGCC 5 cut(s) 529, 567, 765, 1153, 1596
BshNI GGYRCC 1 cut(s) 851
BshVI ATCGAT 2 cut(s) 194, 198
BsiEI CGRYCG 1 cut(s) 198
BsiHKAI GWGCWC 1 cut(s) 828
BsiHKCI CYCGRG 1 cut(s) 766
BsiSI CCGG 4 cut(s) 30, 103, 119, 530
BslI CCNNNNNNNGG 1 cut(s) 80
BsmAI GTCTC 4 cut(s) 151, 333, 584, 1310
BsmBI CGTCTC 1 cut(s) 151
BsnI GGCC 5 cut(s) 529, 567, 765, 1153, 1596
Bso31I GGTCTC 2 cut(s) 333, 584
BsoBI CYCGRG 1 cut(s) 766
Bsp1286I GDGCHC 1 cut(s) 828
Bsp13I TCCGGA 1 cut(s) 102
BspACI CCGC 1 cut(s) 877
BspANI GGCC 5 cut(s) 529, 567, 765, 1153, 1596
BspCNI CTCAG 6 cut(s) 28, 197, 216, 442, 1120, 1516
BspDI ATCGAT 2 cut(s) 194, 198
BspEI TCCGGA 1 cut(s) 102
BspFNI CGCG 2 cut(s) 144, 1389
BspHI TCATGA 1 cut(s) 463
BspLI GGNNCC 3 cut(s) 853, 1134, 1554
BspPI GGATC 5 cut(s) 41, 203, 1364, 1547, 1560
BspT107I GGYRCC 1 cut(s) 851
BspTI CTTAAG 1 cut(s) 317
BspTNI GGTCTC 2 cut(s) 333, 584
BsrDI GCAATG 1 cut(s) 1074
BssECI CCNNGG 3 cut(s) 506, 574, 1407
BssNI GRCGYC 1 cut(s) 852
Bst2UI CCWGG 1 cut(s) 576
Bst4CI ACNGT 6 cut(s) 470, 497, 1067, 1429, 1472, 1483
BstACI GRCGYC 1 cut(s) 852
BstAFI CTTAAG 1 cut(s) 317
BstAPI GCANNNNNTGC 1 cut(s) 1022
BstBAI YACGTR 2 cut(s) 758, 1397
BstC8I GCNNGC 3 cut(s) 156, 850, 1086
BstDEI CTNAG 7 cut(s) 15, 184, 203, 450, 1128, 1320, 1524
BstEII GGTNACC 1 cut(s) 353
BstF5I GGATG 2 cut(s) 10, 1417
BstFNI CGCG 2 cut(s) 144, 1389
BstH2I RGCGCY 1 cut(s) 855
BstHHI GCGC 2 cut(s) 854, 1391
BstMAI GTCTC 4 cut(s) 151, 333, 584, 1310
BstMCI CGRYCG 1 cut(s) 198
BstMWI GCNNNNNNNGC 8 cut(s) 823, 989, 1022, 1085, 1094, 1226, 1265, 1397
BstNI CCWGG 1 cut(s) 576
BstNSI RCATGY 1 cut(s) 817
BstPI GGTNACC 1 cut(s) 353
BstSCI CCNGG 2 cut(s) 29, 574
BstSFI CTRYAG 1 cut(s) 900
BstUI CGCG 2 cut(s) 144, 1389
BstV1I GCAGC 1 cut(s) 532
BstX2I RGATCY 4 cut(s) 33, 113, 679, 1552
BstYI RGATCY 4 cut(s) 33, 113, 679, 1552
Bsu15I ATCGAT 2 cut(s) 194, 198
Bsu36I CCTNAGG 1 cut(s) 1128
BsuI GTATCC 3 cut(s) 374, 1427, 1460
BsuRI GGCC 5 cut(s) 529, 567, 765, 1153, 1596
BsuTUI ATCGAT 2 cut(s) 194, 198
BtsCI GGATG 2 cut(s) 10, 1417
BtsIMutI CAGTG 5 cut(s) 270, 1072, 1477, 1521, 1554
Cac8I GCNNGC 3 cut(s) 156, 850, 1086
CciI TCATGA 1 cut(s) 463
CfoI GCGC 2 cut(s) 854, 1391
Cfr13I GGNCC 4 cut(s) 503, 764, 1151, 1594
ClaI ATCGAT 2 cut(s) 194, 198
CseI GACGC 1 cut(s) 133
Csp6I GTAC 4 cut(s) 1180, 1283, 1344, 1394
CspCI CAANNNNNGTGG 4 cut(s) 690, 725, 1530, 1565
CviAII CATG 9 cut(s) 377, 464, 793, 814, 845, 1031, 1148, 1381, 1451
CviQI GTAC 4 cut(s) 1180, 1283, 1344, 1394
DdeI CTNAG 7 cut(s) 15, 184, 203, 450, 1128, 1320, 1524
DinI GGCGCC 1 cut(s) 853
DraIII CACNNNGTG 1 cut(s) 1022
DriI GACNNNNNGTC 1 cut(s) 602
Eam1105I GACNNNNNGTC 1 cut(s) 602
Eco31I GGTCTC 2 cut(s) 333, 584
Eco47I GGWCC 1 cut(s) 503
Eco57I CTGAAG 2 cut(s) 312, 508
Eco72I CACGTG 1 cut(s) 758
Eco81I CCTNAGG 1 cut(s) 1128
Eco88I CYCGRG 1 cut(s) 766
Eco91I GGTNACC 1 cut(s) 353
EcoO65I GGTNACC 1 cut(s) 353
EcoRII CCWGG 1 cut(s) 574
EcoT22I ATGCAT 2 cut(s) 1270, 1510
EgeI GGCGCC 1 cut(s) 853
EheI GGCGCC 1 cut(s) 853
Esp3I CGTCTC 1 cut(s) 151
FaeI CATG 9 cut(s) 380, 467, 796, 817, 848, 1034, 1151, 1384, 1454
FalI AAGNNNNNCTT 2 cut(s) 506, 538
FatI CATG 9 cut(s) 376, 463, 792, 813, 844, 1030, 1147, 1380, 1450
FauNDI CATATG 1 cut(s) 942
FbaI TGATCA 1 cut(s) 460
FblI GTMKAC 2 cut(s) 786, 1110
Fnu4HI GCNGC 2 cut(s) 546, 877
FokI GGATG 2 cut(s) 17, 1424
Fsp4HI GCNGC 2 cut(s) 546, 877
FspBI CTAG 1 cut(s) 1094
GlaI GCGC 2 cut(s) 853, 1390
GluI GCNGC 2 cut(s) 546, 877
GsuI CTGGAG 2 cut(s) 465, 753
HaeII RGCGCY 1 cut(s) 855
HaeIII GGCC 5 cut(s) 529, 567, 765, 1153, 1596
HapII CCGG 4 cut(s) 30, 103, 119, 530
HgaI GACGC 1 cut(s) 133
HhaI GCGC 2 cut(s) 854, 1391
Hin1I GRCGYC 1 cut(s) 852
Hin1II CATG 9 cut(s) 380, 467, 796, 817, 848, 1034, 1151, 1384, 1454
Hin6I GCGC 2 cut(s) 852, 1389
HinP1I GCGC 2 cut(s) 852, 1389
HincII GTYRAC 2 cut(s) 787, 1111
HindII GTYRAC 2 cut(s) 787, 1111
HpaII CCGG 4 cut(s) 30, 103, 119, 530
HphI GGTGA 5 cut(s) 79, 133, 423, 485, 1301
Hpy166II GTNNAC 3 cut(s) 787, 1103, 1111
Hpy188I TCNGA 8 cut(s) 163, 333, 364, 507, 596, 673, 717, 1411
Hpy8I GTNNAC 3 cut(s) 787, 1103, 1111
Hpy99I CGWCG 1 cut(s) 337
HpyAV CCTTC 4 cut(s) 84, 369, 1147, 1217
HpyCH4III ACNGT 6 cut(s) 470, 497, 1067, 1429, 1472, 1483
HpyCH4IV ACGT 3 cut(s) 308, 757, 1396
HpyCH4V TGCA 6 cut(s) 1084, 1268, 1400, 1425, 1508, 1535
HpyF10VI GCNNNNNNNGC 8 cut(s) 823, 989, 1022, 1085, 1094, 1226, 1265, 1397
HpyF3I CTNAG 7 cut(s) 15, 184, 203, 450, 1128, 1320, 1524
HpySE526I ACGT 3 cut(s) 308, 757, 1396
Hsp92I GRCGYC 1 cut(s) 852
Hsp92II CATG 9 cut(s) 380, 467, 796, 817, 848, 1034, 1151, 1384, 1454
HspAI GCGC 2 cut(s) 852, 1389
KasI GGCGCC 1 cut(s) 851
Kpn2I TCCGGA 1 cut(s) 102
Ksp22I TGATCA 1 cut(s) 460
LmnI GCTCC 2 cut(s) 446, 1226
Lsp1109I GCAGC 1 cut(s) 532
LweI GCATC 4 cut(s) 277, 1255, 1277, 1582
MaeI CTAG 1 cut(s) 1094
MaeII ACGT 3 cut(s) 308, 757, 1396
MaeIII GTNAC 3 cut(s) 353, 422, 1061
MboII GAAGA 9 cut(s) 51, 54, 350, 380, 398, 526, 545, 989, 1136
MflI RGATCY 4 cut(s) 33, 113, 679, 1552
MhlI GDGCHC 1 cut(s) 828
MluCI AATT 6 cut(s) 55, 221, 780, 993, 1237, 1530
Mly113I GGCGCC 1 cut(s) 852
MlyI GAGTC 1 cut(s) 78
MmeI TCCRAC 2 cut(s) 356, 1397
Mph1103I ATGCAT 2 cut(s) 1270, 1510
MroI TCCGGA 1 cut(s) 102
MroXI GAANNNNTTC 3 cut(s) 239, 297, 1328
MseI TTAA 6 cut(s) 95, 224, 318, 387, 630, 996
MslI CAYNNNNRTG 1 cut(s) 1079
MspCI CTTAAG 1 cut(s) 317
MspI CCGG 4 cut(s) 30, 103, 119, 530
MspR9I CCNGG 2 cut(s) 31, 576
MvaI CCWGG 1 cut(s) 576
MvnI CGCG 2 cut(s) 144, 1389
MwoI GCNNNNNNNGC 8 cut(s) 823, 989, 1022, 1085, 1094, 1226, 1265, 1397
NarI GGCGCC 1 cut(s) 852
NciI CCSGG 1 cut(s) 31
NdeI CATATG 1 cut(s) 942
NlaIII CATG 9 cut(s) 380, 467, 796, 817, 848, 1034, 1151, 1384, 1454
NlaIV GGNNCC 3 cut(s) 853, 1134, 1554
NsiI ATGCAT 2 cut(s) 1270, 1510
NspI RCATGY 1 cut(s) 817
PagI TCATGA 1 cut(s) 463
PcsI WCGNNNNNNNCGW 2 cut(s) 204, 1393
PdmI GAANNNNTTC 3 cut(s) 239, 297, 1328
PfeI GAWTC 9 cut(s) 124, 166, 252, 490, 646, 807, 1123, 1384, 1476
PfoI TCCNGGA 1 cut(s) 29
PkrI GCNGC 2 cut(s) 547, 878
Ple19I CGATCG 1 cut(s) 198
PleI GAGTC 1 cut(s) 78
PluTI GGCGCC 1 cut(s) 855
PmaCI CACGTG 1 cut(s) 758
PmlI CACGTG 1 cut(s) 758
PpsI GAGTC 1 cut(s) 78
Ppu21I YACGTR 2 cut(s) 758, 1397
PshBI ATTAAT 1 cut(s) 387
Psp1406I AACGTT 1 cut(s) 308
Psp6I CCWGG 1 cut(s) 574
PspCI CACGTG 1 cut(s) 758
PspEI GGTNACC 1 cut(s) 353
PspGI CCWGG 1 cut(s) 574
PspN4I GGNNCC 3 cut(s) 853, 1134, 1554
PspPI GGNCC 4 cut(s) 503, 764, 1151, 1594
PsuI RGATCY 4 cut(s) 33, 113, 679, 1552
PvuI CGATCG 1 cut(s) 198
RsaI GTAC 4 cut(s) 1181, 1284, 1345, 1395
RsaNI GTAC 4 cut(s) 1180, 1283, 1344, 1394
RseI CAYNNNNRTG 1 cut(s) 1079
SalI GTCGAC 2 cut(s) 785, 1109
SaqAI TTAA 6 cut(s) 95, 224, 318, 387, 630, 996
SatI GCNGC 2 cut(s) 546, 877
Sau96I GGNCC 4 cut(s) 503, 764, 1151, 1594
ScaI AGTACT 1 cut(s) 1345
SchI GAGTC 1 cut(s) 78
ScrFI CCNGG 2 cut(s) 31, 576
SduI GDGCHC 1 cut(s) 828
SfaNI GCATC 4 cut(s) 277, 1255, 1277, 1582
SfcI CTRYAG 1 cut(s) 900
SfoI GGCGCC 1 cut(s) 853
SinI GGWCC 1 cut(s) 503
SmiMI CAYNNNNRTG 1 cut(s) 1079
SmlI CTYRAG 4 cut(s) 317, 410, 614, 1244
SmoI CTYRAG 4 cut(s) 317, 410, 614, 1244
Sse9I AATT 6 cut(s) 55, 221, 780, 993, 1237, 1530
SsiI CCGC 1 cut(s) 877
SspDI GGCGCC 1 cut(s) 851
SspI AATATT 1 cut(s) 721
SspMI CTAG 1 cut(s) 1094
StyD4I CCNGG 2 cut(s) 29, 574
TaaI ACNGT 6 cut(s) 470, 497, 1067, 1429, 1472, 1483
TaiI ACGT 3 cut(s) 311, 760, 1399
TaqI TCGA 9 cut(s) 110, 194, 198, 243, 661, 786, 924, 1110, 1564
TasI AATT 6 cut(s) 55, 221, 780, 993, 1237, 1530
TatI WGTACW 1 cut(s) 1343
TauI GCSGC 1 cut(s) 879
TfiI GAWTC 9 cut(s) 124, 166, 252, 490, 646, 807, 1123, 1384, 1476
Tru1I TTAA 6 cut(s) 95, 224, 318, 387, 630, 996
Tru9I TTAA 6 cut(s) 95, 224, 318, 387, 630, 996
TscAI CASTG 5 cut(s) 277, 1072, 1477, 1528, 1554
TseI GCWGC 1 cut(s) 545
TspDTI ATGAA 7 cut(s) 180, 381, 713, 1063, 1136, 1439, 1597
TspGWI ACGGA 4 cut(s) 28, 96, 350, 852
TspRI CASTG 5 cut(s) 277, 1072, 1477, 1528, 1554
Vha464I CTTAAG 1 cut(s) 317
VpaK11BI GGWCC 1 cut(s) 503
VspI ATTAAT 1 cut(s) 387
XapI RAATTY 1 cut(s) 1237
XceI RCATGY 1 cut(s) 817
XmiI GTMKAC 2 cut(s) 786, 1110
XmnI GAANNNNTTC 3 cut(s) 239, 297, 1328
XspI CTAG 1 cut(s) 1094
ZrmI AGTACT 1 cut(s) 1345
Zsp2I ATGCAT 2 cut(s) 1270, 1510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.