Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT2G22480 |
| fragaria_vesca | FvH4_1g19980 FvH4_1g19980 FvH4_1g19980 FvH4_1g19980 FvH4_1g19980 FvH4_1g19980 |
| malus_domestica | MD01G1037400.v1.1 MD15G1315500.v1.1 |
| prunus_persica | Prupe.6G196200_v2.0.a1 |
| pyrus_communis | pycom01g06530 pycom15g27870 |
| rosa_chinensis | RchiOBHm_Chr2g0111921 |
| rosa_laevigata | RLG00000017938 |
| rosa_multiflora | Rmu_sc0005679.1_g000013 Rmu_sc0006947.1_g000001 |
| rosa_roxburghii | Rroxscaffold_2G00131990 |
| rosa_rugosa | Rorug02G0177100 |
| rosa_samantha | Rh2AG229500 Rh2BG242300 Rh2CG233600 Rh2DG237200 |
| rosa_wichuraiana | Rw2G017760 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 573 |
| Acc65I | GGTACC | 1 cut(s) | 1114 |
| AccB1I | GGYRCC | 1 cut(s) | 1114 |
| AccI | GTMKAC | 1 cut(s) | 1491 |
| AccII | CGCG | 4 cut(s) | 60, 138, 384, 645 |
| AciI | CCGC | 7 cut(s) | 42, 58, 136, 382, 543, 643, 645 |
| AclWI | GGATC | 5 cut(s) | 182, 437, 1189, 1360, 1529 |
| AcsI | RAATTY | 2 cut(s) | 1219, 1398 |
| AcvI | CACGTG | 1 cut(s) | 740 |
| AcyI | GRCGYC | 1 cut(s) | 570 |
| AdeI | CACNNNGTG | 1 cut(s) | 1004 |
| AfaI | GTAC | 4 cut(s) | 387, 454, 1116, 1284 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 169 |
| AgsI | TTSAA | 6 cut(s) | 235, 599, 698, 959, 1124, 1172 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 584 |
| AjnI | CCWGG | 3 cut(s) | 168, 556, 1523 |
| AluBI | AGCT | 5 cut(s) | 668, 723, 836, 1187, 1322 |
| AluI | AGCT | 5 cut(s) | 668, 723, 836, 1187, 1322 |
| Alw26I | GTCTC | 2 cut(s) | 99, 1292 |
| AlwI | GGATC | 5 cut(s) | 182, 437, 1189, 1360, 1529 |
| Ama87I | CYCGRG | 2 cut(s) | 489, 1505 |
| AoxI | GGCC | 2 cut(s) | 485, 1576 |
| ApeKI | GCWGC | 3 cut(s) | 20, 103, 527 |
| ApoI | RAATTY | 2 cut(s) | 1219, 1398 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 920, 952, 1117, 1149 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 270 |
| Asp718I | GGTACC | 1 cut(s) | 1114 |
| AspLEI | GCGC | 2 cut(s) | 62, 231 |
| AspS9I | GGNCC | 3 cut(s) | 485, 746, 1133 |
| AsuHPI | GGTGA | 2 cut(s) | 325, 405 |
| AvaI | CYCGRG | 2 cut(s) | 489, 1505 |
| AvaII | GGWCC | 2 cut(s) | 746, 1133 |
| AxyI | CCTNAGG | 2 cut(s) | 296, 1110 |
| BaeGI | GKGCMC | 1 cut(s) | 8 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 939, 972 |
| BanI | GGYRCC | 1 cut(s) | 1114 |
| BbrPI | CACGTG | 1 cut(s) | 740 |
| BbsI | GAAGAC | 1 cut(s) | 327 |
| BbvI | GCAGC | 3 cut(s) | 7, 90, 539 |
| BccI | CCATC | 4 cut(s) | 349, 1030, 1161, 1388 |
| BceAI | ACGGC | 3 cut(s) | 76, 843, 966 |
| BciT130I | CCWGG | 3 cut(s) | 170, 558, 1525 |
| BcoDI | GTCTC | 2 cut(s) | 99, 1292 |
| BfaI | CTAG | 2 cut(s) | 1080, 1415 |
| BfmI | CTRYAG | 2 cut(s) | 882, 1226 |
| BfoI | RGCGCY | 1 cut(s) | 232 |
| BisI | GCNGC | 5 cut(s) | 21, 42, 104, 136, 528 |
| BlsI | GCNGC | 5 cut(s) | 22, 43, 105, 137, 529 |
| Bme1390I | CCNGG | 3 cut(s) | 170, 558, 1525 |
| Bme18I | GGWCC | 2 cut(s) | 746, 1133 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 584 |
| BmeT110I | CYCGRG | 2 cut(s) | 489, 1505 |
| BmgT120I | GGNCC | 3 cut(s) | 485, 746, 1133 |
| BmiI | GGNNCC | 2 cut(s) | 748, 1116 |
| BmrFI | CCNGG | 3 cut(s) | 170, 558, 1525 |
| BmrI | ACTGGG | 1 cut(s) | 744 |
| BmsI | GCATC | 6 cut(s) | 88, 1079, 1237, 1259, 1397, 1564 |
| BmuI | ACTGGG | 1 cut(s) | 744 |
| BpiI | GAAGAC | 1 cut(s) | 327 |
| BpmI | CTGGAG | 2 cut(s) | 735, 852 |
| BpuEI | CTTGAG | 1 cut(s) | 377 |
| Bsa29I | ATCGAT | 1 cut(s) | 177 |
| BsaAI | YACGTR | 2 cut(s) | 456, 740 |
| BsaHI | GRCGYC | 1 cut(s) | 570 |
| BsaJI | CCNNGG | 6 cut(s) | 67, 556, 643, 1012, 1506, 1523 |
| BsaWI | WCCGGW | 1 cut(s) | 310 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 169 |
| Bse1I | ACTGG | 2 cut(s) | 200, 750 |
| Bse21I | CCTNAGG | 2 cut(s) | 296, 1110 |
| Bse3DI | GCAATG | 2 cut(s) | 473, 1068 |
| BseBI | CCWGG | 3 cut(s) | 170, 558, 1525 |
| BseCI | ATCGAT | 1 cut(s) | 177 |
| BseDI | CCNNGG | 6 cut(s) | 67, 556, 643, 1012, 1506, 1523 |
| BseGI | GGATG | 6 cut(s) | 79, 237, 306, 352, 1070, 1519 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 169 |
| BseMI | GCAATG | 2 cut(s) | 473, 1068 |
| BseMII | CTCAG | 4 cut(s) | 89, 164, 310, 1101 |
| BseNI | ACTGG | 2 cut(s) | 200, 750 |
| BseSI | GKGCMC | 1 cut(s) | 8 |
| BseXI | GCAGC | 3 cut(s) | 7, 90, 539 |
| BseYI | CCCAGC | 2 cut(s) | 482, 1445 |
| Bsh1236I | CGCG | 4 cut(s) | 60, 138, 384, 645 |
| Bsh1285I | CGRYCG | 1 cut(s) | 382 |
| BshFI | GGCC | 2 cut(s) | 487, 1578 |
| BshNI | GGYRCC | 1 cut(s) | 1114 |
| BshVI | ATCGAT | 1 cut(s) | 177 |
| BsiEI | CGRYCG | 1 cut(s) | 382 |
| BsiHKCI | CYCGRG | 2 cut(s) | 489, 1505 |
| BsiSI | CCGG | 1 cut(s) | 311 |
| BslFI | GGGAC | 2 cut(s) | 125, 732 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 169 |
| BsmAI | GTCTC | 2 cut(s) | 99, 1292 |
| BsmBI | CGTCTC | 1 cut(s) | 99 |
| BsmFI | GGGAC | 2 cut(s) | 125, 732 |
| BsmI | GAATGC | 2 cut(s) | 440, 1439 |
| BsnI | GGCC | 2 cut(s) | 487, 1578 |
| BsoBI | CYCGRG | 2 cut(s) | 489, 1505 |
| Bsp1286I | GDGCHC | 1 cut(s) | 8 |
| Bsp143I | GATC | 5 cut(s) | 187, 442, 1181, 1365, 1534 |
| Bsp19I | CCATGG | 2 cut(s) | 67, 1012 |
| BspACI | CCGC | 7 cut(s) | 42, 58, 136, 382, 543, 643, 645 |
| BspANI | GGCC | 2 cut(s) | 487, 1578 |
| BspCNI | CTCAG | 4 cut(s) | 90, 163, 309, 1102 |
| BspDI | ATCGAT | 1 cut(s) | 177 |
| BspFNI | CGCG | 4 cut(s) | 60, 138, 384, 645 |
| BspLI | GGNNCC | 2 cut(s) | 748, 1116 |
| BspPI | GGATC | 5 cut(s) | 182, 437, 1189, 1360, 1529 |
| BspQI | GCTCTTC | 1 cut(s) | 125 |
| BspT107I | GGYRCC | 1 cut(s) | 1114 |
| BsrDI | GCAATG | 2 cut(s) | 473, 1068 |
| BsrI | ACTGG | 2 cut(s) | 200, 750 |
| BssECI | CCNNGG | 6 cut(s) | 67, 556, 643, 1012, 1506, 1523 |
| BssMI | GATC | 5 cut(s) | 187, 442, 1181, 1365, 1534 |
| BssNAI | GTATAC | 1 cut(s) | 1492 |
| BssNI | GRCGYC | 1 cut(s) | 570 |
| BssT1I | CCWWGG | 2 cut(s) | 67, 1012 |
| Bst1107I | GTATAC | 1 cut(s) | 1492 |
| Bst2UI | CCWGG | 3 cut(s) | 170, 558, 1525 |
| Bst4CI | ACNGT | 5 cut(s) | 452, 773, 870, 1133, 1465 |
| Bst6I | CTCTTC | 2 cut(s) | 125, 345 |
| BstACI | GRCGYC | 1 cut(s) | 570 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 1004, 1379 |
| BstBAI | YACGTR | 2 cut(s) | 456, 740 |
| BstC8I | GCNNGC | 4 cut(s) | 18, 108, 1068, 1580 |
| BstDEI | CTNAG | 4 cut(s) | 98, 150, 296, 1110 |
| BstDSI | CCRYGG | 3 cut(s) | 67, 643, 1012 |
| BstEII | GGTNACC | 1 cut(s) | 638 |
| BstF5I | GGATG | 6 cut(s) | 79, 237, 306, 352, 1070, 1519 |
| BstFNI | CGCG | 4 cut(s) | 60, 138, 384, 645 |
| BstH2I | RGCGCY | 1 cut(s) | 232 |
| BstHHI | GCGC | 2 cut(s) | 62, 231 |
| BstKTI | GATC | 5 cut(s) | 190, 445, 1184, 1368, 1537 |
| BstMAI | GTCTC | 2 cut(s) | 99, 1292 |
| BstMBI | GATC | 5 cut(s) | 187, 442, 1181, 1365, 1534 |
| BstMCI | CGRYCG | 1 cut(s) | 382 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 26, 971, 1004, 1067, 1208, 1247, 1379 |
| BstNI | CCWGG | 3 cut(s) | 170, 558, 1525 |
| BstNSI | RCATGY | 5 cut(s) | 20, 543, 830, 1377, 1476 |
| BstPI | GGTNACC | 1 cut(s) | 638 |
| BstSCI | CCNGG | 3 cut(s) | 168, 556, 1523 |
| BstSFI | CTRYAG | 2 cut(s) | 882, 1226 |
| BstSLI | GKGCMC | 1 cut(s) | 8 |
| BstUI | CGCG | 4 cut(s) | 60, 138, 384, 645 |
| BstV1I | GCAGC | 3 cut(s) | 7, 90, 539 |
| BstV2I | GAAGAC | 1 cut(s) | 327 |
| BstX2I | RGATCY | 1 cut(s) | 1534 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1019 |
| BstYI | RGATCY | 1 cut(s) | 1534 |
| BstZ17I | GTATAC | 1 cut(s) | 1492 |
| Bsu15I | ATCGAT | 1 cut(s) | 177 |
| Bsu36I | CCTNAGG | 2 cut(s) | 296, 1110 |
| BsuRI | GGCC | 2 cut(s) | 487, 1578 |
| BsuTUI | ATCGAT | 1 cut(s) | 177 |
| BtgI | CCRYGG | 3 cut(s) | 67, 643, 1012 |
| BtsCI | GGATG | 6 cut(s) | 79, 237, 306, 352, 1070, 1519 |
| BtsI | GCAGTG | 1 cut(s) | 1054 |
| BtsIMutI | CAGTG | 3 cut(s) | 249, 757, 1054 |
| Cac8I | GCNNGC | 4 cut(s) | 18, 108, 1068, 1580 |
| CfoI | GCGC | 2 cut(s) | 62, 231 |
| Cfr13I | GGNCC | 3 cut(s) | 485, 746, 1133 |
| Cfr42I | CCGCGG | 1 cut(s) | 646 |
| ClaI | ATCGAT | 1 cut(s) | 177 |
| CseI | GACGC | 2 cut(s) | 127, 848 |
| Csp6I | GTAC | 4 cut(s) | 386, 453, 1115, 1283 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 672, 707 |
| CviQI | GTAC | 4 cut(s) | 386, 453, 1115, 1283 |
| DdeI | CTNAG | 4 cut(s) | 98, 150, 296, 1110 |
| DpnI | GATC | 5 cut(s) | 189, 444, 1183, 1367, 1536 |
| DpnII | GATC | 5 cut(s) | 187, 442, 1181, 1365, 1534 |
| DraI | TTTAAA | 2 cut(s) | 508, 1315 |
| DraIII | CACNNNGTG | 1 cut(s) | 1004 |
| DriI | GACNNNNNGTC | 1 cut(s) | 584 |
| Eam1104I | CTCTTC | 2 cut(s) | 125, 345 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 584 |
| EarI | CTCTTC | 2 cut(s) | 125, 345 |
| Eco130I | CCWWGG | 2 cut(s) | 67, 1012 |
| Eco47I | GGWCC | 2 cut(s) | 746, 1133 |
| Eco72I | CACGTG | 1 cut(s) | 740 |
| Eco81I | CCTNAGG | 2 cut(s) | 296, 1110 |
| Eco88I | CYCGRG | 2 cut(s) | 489, 1505 |
| Eco91I | GGTNACC | 1 cut(s) | 638 |
| EcoO109I | RGGNCCY | 1 cut(s) | 746 |
| EcoO65I | GGTNACC | 1 cut(s) | 638 |
| EcoRI | GAATTC | 1 cut(s) | 1398 |
| EcoRII | CCWGG | 3 cut(s) | 168, 556, 1523 |
| EcoT14I | CCWWGG | 2 cut(s) | 67, 1012 |
| EcoT22I | ATGCAT | 3 cut(s) | 440, 1252, 1390 |
| ErhI | CCWWGG | 2 cut(s) | 67, 1012 |
| Esp3I | CGTCTC | 1 cut(s) | 99 |
| FaqI | GGGAC | 2 cut(s) | 125, 732 |
| FauNDI | CATATG | 1 cut(s) | 1097 |
| FblI | GTMKAC | 1 cut(s) | 1491 |
| Fnu4HI | GCNGC | 5 cut(s) | 21, 42, 104, 136, 528 |
| FokI | GGATG | 6 cut(s) | 66, 224, 313, 359, 1057, 1506 |
| Fsp4HI | GCNGC | 5 cut(s) | 21, 42, 104, 136, 528 |
| FspBI | CTAG | 2 cut(s) | 1080, 1415 |
| GlaI | GCGC | 2 cut(s) | 61, 230 |
| GluI | GCNGC | 5 cut(s) | 21, 42, 104, 136, 528 |
| GsaI | CCCAGC | 2 cut(s) | 486, 1449 |
| GsuI | CTGGAG | 2 cut(s) | 735, 852 |
| HaeII | RGCGCY | 1 cut(s) | 232 |
| HaeIII | GGCC | 2 cut(s) | 487, 1578 |
| HapII | CCGG | 1 cut(s) | 311 |
| HgaI | GACGC | 2 cut(s) | 127, 848 |
| HhaI | GCGC | 2 cut(s) | 62, 231 |
| Hin1I | GRCGYC | 1 cut(s) | 570 |
| Hin6I | GCGC | 2 cut(s) | 60, 229 |
| HinP1I | GCGC | 2 cut(s) | 60, 229 |
| HindIII | AAGCTT | 1 cut(s) | 721 |
| HinfI | GANTC | 7 cut(s) | 36, 266, 307, 628, 789, 1053, 1458 |
| HpaII | CCGG | 1 cut(s) | 311 |
| HphI | GGTGA | 2 cut(s) | 325, 405 |
| Hpy166II | GTNNAC | 3 cut(s) | 769, 1085, 1492 |
| Hpy188I | TCNGA | 8 cut(s) | 84, 99, 153, 346, 578, 655, 1158, 1393 |
| Hpy188III | TCNNGA | 9 cut(s) | 191, 211, 223, 298, 394, 491, 714, 1254, 1505 |
| Hpy8I | GTNNAC | 3 cut(s) | 769, 1085, 1492 |
| HpyAV | CCTTC | 4 cut(s) | 154, 291, 298, 1505 |
| HpyCH4III | ACNGT | 5 cut(s) | 452, 773, 870, 1133, 1465 |
| HpyCH4IV | ACGT | 4 cut(s) | 255, 455, 570, 739 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 26, 971, 1004, 1067, 1208, 1247, 1379 |
| HpyF3I | CTNAG | 4 cut(s) | 98, 150, 296, 1110 |
| HpySE526I | ACGT | 4 cut(s) | 255, 455, 570, 739 |
| Hsp92I | GRCGYC | 1 cut(s) | 570 |
| HspAI | GCGC | 2 cut(s) | 60, 229 |
| KpnI | GGTACC | 1 cut(s) | 1118 |
| KspI | CCGCGG | 1 cut(s) | 646 |
| Kzo9I | GATC | 5 cut(s) | 187, 442, 1181, 1365, 1534 |
| LguI | GCTCTTC | 1 cut(s) | 125 |
| LmnI | GCTCC | 3 cut(s) | 463, 833, 1208 |
| Lsp1109I | GCAGC | 3 cut(s) | 7, 90, 539 |
| LweI | GCATC | 6 cut(s) | 88, 1079, 1237, 1259, 1397, 1564 |
| MaeI | CTAG | 2 cut(s) | 1080, 1415 |
| MaeII | ACGT | 4 cut(s) | 255, 455, 570, 739 |
| MaeIII | GTNAC | 4 cut(s) | 362, 411, 535, 638 |
| MalI | GATC | 5 cut(s) | 189, 444, 1183, 1367, 1536 |
| MboI | GATC | 5 cut(s) | 187, 442, 1181, 1365, 1534 |
| MboII | GAAGA | 5 cut(s) | 142, 332, 362, 527, 971 |
| MfeI | CAATTG | 1 cut(s) | 531 |
| MflI | RGATCY | 1 cut(s) | 1534 |
| MhlI | GDGCHC | 1 cut(s) | 8 |
| MluCI | AATT | 7 cut(s) | 531, 662, 1089, 1167, 1219, 1398, 1500 |
| MlyI | GAGTC | 2 cut(s) | 30, 301 |
| Mph1103I | ATGCAT | 3 cut(s) | 440, 1252, 1390 |
| MroXI | GAANNNNTTC | 1 cut(s) | 270 |
| MseI | TTAA | 4 cut(s) | 507, 564, 872, 1314 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1061, 1361 |
| MspA1I | CMGCKG | 2 cut(s) | 645, 1187 |
| MspI | CCGG | 1 cut(s) | 311 |
| MspR9I | CCNGG | 3 cut(s) | 170, 558, 1525 |
| MunI | CAATTG | 1 cut(s) | 531 |
| Mva1269I | GAATGC | 2 cut(s) | 440, 1439 |
| MvaI | CCWGG | 3 cut(s) | 170, 558, 1525 |
| MvnI | CGCG | 4 cut(s) | 60, 138, 384, 645 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 26, 971, 1004, 1067, 1208, 1247, 1379 |
| NcoI | CCATGG | 2 cut(s) | 67, 1012 |
| NdeI | CATATG | 1 cut(s) | 1097 |
| NdeII | GATC | 5 cut(s) | 187, 442, 1181, 1365, 1534 |
| NlaIV | GGNNCC | 2 cut(s) | 748, 1116 |
| NmuCI | GTSAC | 1 cut(s) | 411 |
| NsiI | ATGCAT | 3 cut(s) | 440, 1252, 1390 |
| NspI | RCATGY | 5 cut(s) | 20, 543, 830, 1377, 1476 |
| PaeI | GCATGC | 1 cut(s) | 20 |
| PaeR7I | CTCGAG | 1 cut(s) | 489 |
| PciSI | GCTCTTC | 1 cut(s) | 125 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 20, 183, 261 |
| PctI | GAATGC | 2 cut(s) | 440, 1439 |
| PdmI | GAANNNNTTC | 1 cut(s) | 270 |
| PfeI | GAWTC | 5 cut(s) | 266, 628, 789, 1053, 1458 |
| PflFI | GACNNNGTC | 1 cut(s) | 1589 |
| PkrI | GCNGC | 5 cut(s) | 22, 43, 105, 137, 529 |
| PleI | GAGTC | 2 cut(s) | 30, 301 |
| PmaCI | CACGTG | 1 cut(s) | 740 |
| PmlI | CACGTG | 1 cut(s) | 740 |
| PpsI | GAGTC | 2 cut(s) | 30, 301 |
| Ppu21I | YACGTR | 2 cut(s) | 456, 740 |
| PpuMI | RGGWCCY | 1 cut(s) | 746 |
| Psp5II | RGGWCCY | 1 cut(s) | 746 |
| Psp6I | CCWGG | 3 cut(s) | 168, 556, 1523 |
| PspCI | CACGTG | 1 cut(s) | 740 |
| PspEI | GGTNACC | 1 cut(s) | 638 |
| PspFI | CCCAGC | 2 cut(s) | 482, 1445 |
| PspGI | CCWGG | 3 cut(s) | 168, 556, 1523 |
| PspN4I | GGNNCC | 2 cut(s) | 748, 1116 |
| PspPI | GGNCC | 3 cut(s) | 485, 746, 1133 |
| PspPPI | RGGWCCY | 1 cut(s) | 746 |
| PsuI | RGATCY | 1 cut(s) | 1534 |
| PsyI | GACNNNGTC | 1 cut(s) | 1589 |
| PvuII | CAGCTG | 1 cut(s) | 1187 |
| RsaI | GTAC | 4 cut(s) | 387, 454, 1116, 1284 |
| RsaNI | GTAC | 4 cut(s) | 386, 453, 1115, 1283 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1061, 1361 |
| SacII | CCGCGG | 1 cut(s) | 646 |
| SapI | GCTCTTC | 1 cut(s) | 125 |
| SaqAI | TTAA | 4 cut(s) | 507, 564, 872, 1314 |
| SatI | GCNGC | 5 cut(s) | 21, 42, 104, 136, 528 |
| Sau3AI | GATC | 5 cut(s) | 187, 442, 1181, 1365, 1534 |
| Sau96I | GGNCC | 3 cut(s) | 485, 746, 1133 |
| SchI | GAGTC | 2 cut(s) | 30, 301 |
| ScrFI | CCNGG | 3 cut(s) | 170, 558, 1525 |
| SduI | GDGCHC | 1 cut(s) | 8 |
| SfaNI | GCATC | 6 cut(s) | 88, 1079, 1237, 1259, 1397, 1564 |
| SfcI | CTRYAG | 2 cut(s) | 882, 1226 |
| Sfr274I | CTCGAG | 1 cut(s) | 489 |
| Sfr303I | CCGCGG | 1 cut(s) | 646 |
| SgrBI | CCGCGG | 1 cut(s) | 646 |
| SinI | GGWCC | 2 cut(s) | 746, 1133 |
| SlaI | CTCGAG | 1 cut(s) | 489 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1061, 1361 |
| SmlI | CTYRAG | 2 cut(s) | 392, 489 |
| SmoI | CTYRAG | 2 cut(s) | 392, 489 |
| SphI | GCATGC | 1 cut(s) | 20 |
| Sse9I | AATT | 7 cut(s) | 531, 662, 1089, 1167, 1219, 1398, 1500 |
| SsiI | CCGC | 7 cut(s) | 42, 58, 136, 382, 543, 643, 645 |
| SspI | AATATT | 1 cut(s) | 1310 |
| SspMI | CTAG | 2 cut(s) | 1080, 1415 |
| StyD4I | CCNGG | 3 cut(s) | 168, 556, 1523 |
| StyI | CCWWGG | 2 cut(s) | 67, 1012 |
| TaaI | ACNGT | 5 cut(s) | 452, 773, 870, 1133, 1465 |
| TaiI | ACGT | 4 cut(s) | 258, 458, 573, 742 |
| TaqI | TCGA | 3 cut(s) | 177, 222, 490 |
| TasI | AATT | 7 cut(s) | 531, 662, 1089, 1167, 1219, 1398, 1500 |
| TauI | GCSGC | 2 cut(s) | 44, 138 |
| TfiI | GAWTC | 5 cut(s) | 266, 628, 789, 1053, 1458 |
| Tru1I | TTAA | 4 cut(s) | 507, 564, 872, 1314 |
| Tru9I | TTAA | 4 cut(s) | 507, 564, 872, 1314 |
| TscAI | CASTG | 3 cut(s) | 256, 757, 1054 |
| TseFI | GTSAC | 1 cut(s) | 411 |
| TseI | GCWGC | 3 cut(s) | 20, 103, 527 |
| Tsp45I | GTSAC | 1 cut(s) | 411 |
| TspDTI | ATGAA | 5 cut(s) | 363, 464, 695, 1045, 1421 |
| TspGWI | ACGGA | 1 cut(s) | 1358 |
| TspRI | CASTG | 3 cut(s) | 256, 757, 1054 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1589 |
| VpaK11BI | GGWCC | 2 cut(s) | 746, 1133 |
| XapI | RAATTY | 2 cut(s) | 1219, 1398 |
| XceI | RCATGY | 5 cut(s) | 20, 543, 830, 1377, 1476 |
| XhoI | CTCGAG | 1 cut(s) | 489 |
| XmiI | GTMKAC | 1 cut(s) | 1491 |
| XmnI | GAANNNNTTC | 1 cut(s) | 270 |
| XspI | CTAG | 2 cut(s) | 1080, 1415 |
| ZraI | GACGTC | 1 cut(s) | 571 |
| Zsp2I | ATGCAT | 3 cut(s) | 440, 1252, 1390 |