Rroxscaffold_2G00131990

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0008632
Physical Location & Seq
Forward (+)
68819902 .. 68825345
5444 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00131990.1

Sequence Viewer

Length: 1827 bp
ATGCGATCGCGCCCAGGCTCGCCGCCCTCCTCCACCACCACCACCACCGTCACGGTAATGGAGTATTCTCCTCCTCCTCCTCCGTCTCTTCTTCTTCTCGAACCTCGCGCTCTTCTTCTTCTTCTGCTTCGCTTCGGATTGACGACGAAGAAGAAGAGCAGCAGCGTTCGAGTCTTGGCCGGGATCAAGCACAACCACCGCGACCTCGATTTCAGCGATCCTGACTGGAAATCAAAGTTCCAGAGCGATTTCGAGAAGCGCTTCGCCATCCCTCACATCACCGACGTCTTCCCCGACGCCGTTCCCATTCCCTCCACCTTCTGCCTCAAGATGAGAACTCCGGTCATCGAAGACTTCGCCGGCGGATATCCGTCCGACGAGGAGTGGCACGGTTACATAAATAACAACGATCGCGTACTTCTCAAGACTATATACTACTCGTCACCTACGTCTGCTGGTGCTGAATGCATTGATCCTAACTTTTCTATTGTGTTTGCACTATGTAATGTTCAGCTCATCATAAAAGCAGCATATGAGCTACTCTGCTTTAAAATTTTGCGTGAAAAAGAGTGCAACTTGAGTTTAAACATGTTGTATGCATCTCTTCTTAGACGCGTGGCTTGCTTAACATATGGGATGGTAGGTTGGATTTTCCAGCTGGACAAAGAAACTTGTGATGAATATGAGCTGAAATTTTACAGGGTTCATCGTGCCGGGCCACGAGAAAAGATATATTTTAAACCTGAAGAAGTGAAAGCAGCAATTGTAACATGCGGAGGGCTCTGTCCTGGTCTTAATGATGTCATCAGACAGATTGTCATTACACTTGAAATATATGGTGTAAAAAAGATTGTTGGTATTCCTTACGGTTATCGTGGATTTTCTGATCCAGAATTAGCCGAAATGCCACTCTCGAGGAAGGTGGTTCAAAATGTTCATCTTTCTGGTGGAAGCTTGTTAGGAGTTTCACGTGGAGGCCCCAGCGTTAGTGAAATAGTGGACAATTTAGAGGAAAGAGGGATCAATATGCTCTTTGTTTTGGGTGGAAACGGCACACATGCTGGAGCCGATGCAATACACACTGAGTGCCGCAAAAGACAGTTAAGGGTGGCTATAGTTGGTGTGCCGAAAACCATAGACAATGACATTTTGTTAATGGACAAAACATTTGGTTTCGATACTGCTGTTGAAGAAGCCCAGCGAGCAATAAATTCAGCATATATTGAGGCACATAGTGCTTACCATGGTATTGGCGTTGTGAAATTGATGGGTCGTAGCAGTGGATTCATAGCAATGCACGCATCCCTGGCTAGTGGACAAATTGACATATGTTTGATACCTGAGGTACCTTTTCAAATACATGGCCCTCATGGTATTCTGCACCATCTGAAATACCTGATTGAAACAAAGGGATCAGCTGTTGTCTGTGTAGCAGAGGGAGCAGGGCAGAATTTACTACAGAAAACCAATGCTACTGATGCATCAGGAAATGTTGTATTTGGGGATATTGGAGTACATATTCAACAAGAGACAAAGAAACATTTTAAGGAGCATGGTGTTCCTGTTGATGTGAAATATATAGACCCCACATACATGATTCGTGCATGTCGTGCAAATGCATCAGATGGAATTCTTTGCGCTGTGCTAGGGCAGAATGCCGTTCATGGTGCATTTGCTGGATATAGTGGCATCACAATTGGCATATGCAACACTCATTATGTATATCTTCCGATTCCTGAGGTTATTTCTCATCCCCGACTGGTGGATCCCAACAGTCGAATGTGGCACCGTTGCTTGACTTCAACAGGCCAGCCAGACTTCATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003872 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008443 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009536 GO:0009987 GO:0016052 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019200 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044262 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046835 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0061615 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko00051 ko00052 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04152 ko05230 map00010 map00030 map00051 map00052 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04152 map05230
Pfam Domains
Protein Families
PFK

Protein Analysis

608

Amino Acids

66.99

Weight (kDa)

7.3

Isoelectric Point (pI)

38.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PFK PF00365 253 - 555 9.4e-62 Phosphofructokinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 288
Acc65I GGTACC 1 cut(s) 1345
AccB1I GGYRCC 2 cut(s) 1345, 1785
AccII CGCG 5 cut(s) 10, 108, 201, 414, 615
AciI CCGC 5 cut(s) 23, 199, 363, 774, 1090
AclWI GGATC 8 cut(s) 191, 212, 467, 881, 1028, 1420, 1760, 1773
AcoI YGGCCR 1 cut(s) 177
AcsI RAATTY 5 cut(s) 552, 692, 1210, 1450, 1629
AcuI CTGAAG 1 cut(s) 765
AcvI CACGTG 1 cut(s) 971
AcyI GRCGYC 2 cut(s) 285, 297
AdeI CACNNNGTG 2 cut(s) 1086, 1235
AfaI GTAC 3 cut(s) 417, 1347, 1515
AfeI AGCGCT 1 cut(s) 260
AfiI CCNNNNNNNGG 1 cut(s) 1762
AflIII ACRYGT 2 cut(s) 588, 613
AgsI TTSAA 7 cut(s) 830, 929, 1190, 1355, 1403, 1523, 1803
AhdI GACNNNNNGTC 1 cut(s) 815
AjnI CCWGG 3 cut(s) 13, 787, 1305
AluBI AGCT 6 cut(s) 514, 538, 658, 688, 954, 1418
AluI AGCT 6 cut(s) 514, 538, 658, 688, 954, 1418
Alw26I GTCTC 2 cut(s) 90, 1523
AlwI GGATC 8 cut(s) 191, 212, 467, 881, 1028, 1420, 1760, 1773
Ama87I CYCGRG 1 cut(s) 913
Aor51HI AGCGCT 1 cut(s) 260
AoxI GGCC 5 cut(s) 177, 716, 976, 1363, 1807
ApeKI GCWGC 4 cut(s) 159, 162, 527, 758
ApoI RAATTY 5 cut(s) 552, 692, 1210, 1450, 1629
ArsI GACNNNNNNTTYG 2 cut(s) 1151, 1183
AsiSI GCGATCGC 1 cut(s) 8
Asp700I GAANNNNTTC 1 cut(s) 260
Asp718I GGTACC 1 cut(s) 1345
AspLEI GCGC 4 cut(s) 12, 110, 261, 1640
AspS9I GGNCC 3 cut(s) 716, 977, 1364
AsuC2I CCSGG 2 cut(s) 181, 715
AsuHPI GGTGA 2 cut(s) 271, 435
AvaI CYCGRG 1 cut(s) 913
AxyI CCTNAGG 2 cut(s) 1341, 1737
BaeI ACNNNNGTAYC 2 cut(s) 1170, 1203
BamHI GGATCC 1 cut(s) 1765
BanI GGYRCC 2 cut(s) 1345, 1785
BanII GRGCYC 1 cut(s) 783
BauI CACGAG 1 cut(s) 720
BbrPI CACGTG 1 cut(s) 971
BbsI GAAGAC 2 cut(s) 280, 357
BbvI GCAGC 4 cut(s) 171, 174, 539, 770
BccI CCATC 5 cut(s) 275, 631, 1261, 1392, 1619
BceAI ACGGC 3 cut(s) 284, 1066, 1643
BciT130I CCWGG 3 cut(s) 15, 789, 1307
BcnI CCSGG 2 cut(s) 181, 715
BcoDI GTCTC 2 cut(s) 90, 1523
BfaI CTAG 2 cut(s) 1311, 1646
BfmI CTRYAG 2 cut(s) 1113, 1457
BfoI RGCGCY 1 cut(s) 262
BisI GCNGC 6 cut(s) 23, 160, 163, 528, 759, 1090
BlsI GCNGC 6 cut(s) 24, 161, 164, 529, 760, 1091
Bme1390I CCNGG 5 cut(s) 15, 181, 715, 789, 1307
BmeRI GACNNNNNGTC 1 cut(s) 815
BmeT110I CYCGRG 1 cut(s) 913
BmgT120I GGNCC 3 cut(s) 716, 977, 1364
BmiI GGNNCC 5 cut(s) 979, 1066, 1347, 1767, 1787
BmrFI CCNGG 5 cut(s) 15, 181, 715, 789, 1307
BmsI GCATC 7 cut(s) 608, 1060, 1310, 1468, 1490, 1628, 1698
BpiI GAAGAC 2 cut(s) 280, 357
BpmI CTGGAG 1 cut(s) 1083
BpuEI CTTGAG 3 cut(s) 311, 407, 598
BpuMI CCSGG 2 cut(s) 181, 715
BsaAI YACGTR 1 cut(s) 971
BsaHI GRCGYC 2 cut(s) 285, 297
BsaJI CCNNGG 3 cut(s) 13, 1243, 1305
BsaWI WCCGGW 1 cut(s) 340
Bsc4I CCNNNNNNNGG 1 cut(s) 1762
Bse118I RCCGGY 1 cut(s) 359
Bse1I ACTGG 2 cut(s) 230, 1764
Bse21I CCTNAGG 2 cut(s) 1341, 1737
Bse3DI GCAATG 1 cut(s) 1299
BseBI CCWGG 3 cut(s) 15, 789, 1307
BseDI CCNNGG 3 cut(s) 13, 1243, 1305
BseGI GGATG 4 cut(s) 267, 642, 1301, 1750
BseLI CCNNNNNNNGG 1 cut(s) 1762
BseMI GCAATG 1 cut(s) 1299
BseMII CTCAG 3 cut(s) 1074, 1332, 1728
BseNI ACTGG 2 cut(s) 230, 1764
BseRI GAGGAG 6 cut(s) 19, 60, 63, 66, 69, 395
BseXI GCAGC 4 cut(s) 171, 174, 539, 770
BseYI CCCAGC 2 cut(s) 980, 1197
BsgI GTGCAG 1 cut(s) 1364
Bsh1236I CGCG 5 cut(s) 10, 108, 201, 414, 615
Bsh1285I CGRYCG 2 cut(s) 8, 412
BshFI GGCC 5 cut(s) 179, 718, 978, 1365, 1809
BshNI GGYRCC 2 cut(s) 1345, 1785
BsiEI CGRYCG 2 cut(s) 8, 412
BsiHKCI CYCGRG 1 cut(s) 913
BsiSI CCGG 4 cut(s) 180, 341, 360, 714
BslI CCNNNNNNNGG 1 cut(s) 1762
BsmAI GTCTC 2 cut(s) 90, 1523
BsmBI CGTCTC 1 cut(s) 90
BsmI GAATGC 2 cut(s) 470, 1660
BsnI GGCC 5 cut(s) 179, 718, 978, 1365, 1809
BsoBI CYCGRG 1 cut(s) 913
Bsp1286I GDGCHC 1 cut(s) 783
Bsp143I GATC 9 cut(s) 5, 183, 217, 409, 472, 886, 1020, 1412, 1765
Bsp19I CCATGG 1 cut(s) 1243
BspACI CCGC 5 cut(s) 23, 199, 363, 774, 1090
BspANI GGCC 5 cut(s) 179, 718, 978, 1365, 1809
BspCNI CTCAG 3 cut(s) 1075, 1333, 1729
BspFNI CGCG 5 cut(s) 10, 108, 201, 414, 615
BspLI GGNNCC 5 cut(s) 979, 1066, 1347, 1767, 1787
BspPI GGATC 8 cut(s) 191, 212, 467, 881, 1028, 1420, 1760, 1773
BspQI GCTCTTC 2 cut(s) 117, 149
BspT107I GGYRCC 2 cut(s) 1345, 1785
BsrDI GCAATG 1 cut(s) 1299
BsrFI RCCGGY 1 cut(s) 359
BsrI ACTGG 2 cut(s) 230, 1764
BssAI RCCGGY 1 cut(s) 359
BssECI CCNNGG 3 cut(s) 13, 1243, 1305
BssMI GATC 9 cut(s) 5, 183, 217, 409, 472, 886, 1020, 1412, 1765
BssNI GRCGYC 2 cut(s) 285, 297
BssSI CACGAG 1 cut(s) 720
BssT1I CCWWGG 1 cut(s) 1243
Bst2BI CACGAG 1 cut(s) 720
Bst2UI CCWGG 3 cut(s) 15, 789, 1307
Bst4CI ACNGT 7 cut(s) 49, 55, 392, 869, 1101, 1775, 1790
Bst6I CTCTTC 4 cut(s) 93, 117, 149, 609
BstACI GRCGYC 2 cut(s) 285, 297
BstAPI GCANNNNNTGC 2 cut(s) 1235, 1610
BstBAI YACGTR 1 cut(s) 971
BstC8I GCNNGC 6 cut(s) 20, 361, 622, 1203, 1299, 1811
BstDEI CTNAG 4 cut(s) 608, 1083, 1341, 1737
BstDSI CCRYGG 1 cut(s) 1243
BstF5I GGATG 4 cut(s) 267, 642, 1301, 1750
BstFNI CGCG 5 cut(s) 10, 108, 201, 414, 615
BstH2I RGCGCY 1 cut(s) 262
BstHHI GCGC 4 cut(s) 12, 110, 261, 1640
BstKTI GATC 9 cut(s) 8, 186, 220, 412, 475, 889, 1023, 1415, 1768
BstMAI GTCTC 2 cut(s) 90, 1523
BstMBI GATC 9 cut(s) 5, 183, 217, 409, 472, 886, 1020, 1412, 1765
BstMCI CGRYCG 2 cut(s) 8, 412
BstMWI GCNNNNNNNGC 8 cut(s) 621, 1202, 1235, 1298, 1307, 1439, 1478, 1610
BstNI CCWGG 3 cut(s) 15, 789, 1307
BstNSI RCATGY 4 cut(s) 592, 774, 1061, 1608
BstSCI CCNGG 5 cut(s) 13, 179, 713, 787, 1305
BstSFI CTRYAG 2 cut(s) 1113, 1457
BstUI CGCG 5 cut(s) 10, 108, 201, 414, 615
BstV1I GCAGC 4 cut(s) 171, 174, 539, 770
BstV2I GAAGAC 2 cut(s) 280, 357
BstX2I RGATCY 1 cut(s) 1765
BstXI CCANNNNNNTGG 1 cut(s) 1250
BstYI RGATCY 1 cut(s) 1765
Bsu36I CCTNAGG 2 cut(s) 1341, 1737
BsuRI GGCC 5 cut(s) 179, 718, 978, 1365, 1809
BtgI CCRYGG 1 cut(s) 1243
BtsCI GGATG 4 cut(s) 267, 642, 1301, 1750
BtsI GCAGTG 1 cut(s) 1285
BtsIMutI CAGTG 2 cut(s) 1080, 1285
Cac8I GCNNGC 6 cut(s) 20, 361, 622, 1203, 1299, 1811
CfoI GCGC 4 cut(s) 12, 110, 261, 1640
Cfr10I RCCGGY 1 cut(s) 359
Cfr13I GGNCC 3 cut(s) 716, 977, 1364
CseI GACGC 2 cut(s) 305, 621
Csp6I GTAC 3 cut(s) 416, 1346, 1514
CviQI GTAC 3 cut(s) 416, 1346, 1514
DdeI CTNAG 4 cut(s) 608, 1083, 1341, 1737
DpnI GATC 9 cut(s) 7, 185, 219, 411, 474, 888, 1022, 1414, 1767
DpnII GATC 9 cut(s) 5, 183, 217, 409, 472, 886, 1020, 1412, 1765
DraI TTTAAA 3 cut(s) 550, 585, 739
DraIII CACNNNGTG 2 cut(s) 1086, 1235
DriI GACNNNNNGTC 1 cut(s) 815
EaeI YGGCCR 1 cut(s) 177
Eam1104I CTCTTC 4 cut(s) 93, 117, 149, 609
Eam1105I GACNNNNNGTC 1 cut(s) 815
EarI CTCTTC 4 cut(s) 93, 117, 149, 609
EciI GGCGGA 1 cut(s) 378
Eco130I CCWWGG 1 cut(s) 1243
Eco24I GRGCYC 1 cut(s) 783
Eco32I GATATC 1 cut(s) 368
Eco47III AGCGCT 1 cut(s) 260
Eco57I CTGAAG 1 cut(s) 765
Eco72I CACGTG 1 cut(s) 971
Eco81I CCTNAGG 2 cut(s) 1341, 1737
Eco88I CYCGRG 1 cut(s) 913
EcoO109I RGGNCCY 1 cut(s) 977
EcoRI GAATTC 1 cut(s) 1629
EcoRII CCWGG 3 cut(s) 13, 787, 1305
EcoRV GATATC 1 cut(s) 368
EcoT14I CCWWGG 1 cut(s) 1243
EcoT22I ATGCAT 4 cut(s) 470, 601, 1483, 1621
EcoT38I GRGCYC 1 cut(s) 783
ErhI CCWWGG 1 cut(s) 1243
Esp3I CGTCTC 1 cut(s) 90
FauNDI CATATG 4 cut(s) 532, 631, 1328, 1703
Fnu4HI GCNGC 6 cut(s) 23, 160, 163, 528, 759, 1090
FokI GGATG 4 cut(s) 254, 649, 1288, 1737
FriOI GRGCYC 1 cut(s) 783
Fsp4HI GCNGC 6 cut(s) 23, 160, 163, 528, 759, 1090
FspBI CTAG 2 cut(s) 1311, 1646
GlaI GCGC 4 cut(s) 11, 109, 260, 1639
GluI GCNGC 6 cut(s) 23, 160, 163, 528, 759, 1090
GsaI CCCAGC 2 cut(s) 984, 1201
GsuI CTGGAG 1 cut(s) 1083
HaeII RGCGCY 1 cut(s) 262
HaeIII GGCC 5 cut(s) 179, 718, 978, 1365, 1809
HapII CCGG 4 cut(s) 180, 341, 360, 714
HgaI GACGC 2 cut(s) 305, 621
HhaI GCGC 4 cut(s) 12, 110, 261, 1640
Hin1I GRCGYC 2 cut(s) 285, 297
Hin6I GCGC 4 cut(s) 10, 108, 259, 1638
HinP1I GCGC 4 cut(s) 10, 108, 259, 1638
HindIII AAGCTT 1 cut(s) 952
HinfI GANTC 4 cut(s) 171, 1284, 1597, 1732
HpaII CCGG 4 cut(s) 180, 341, 360, 714
HphI GGTGA 2 cut(s) 271, 435
Hpy166II GTNNAC 2 cut(s) 1000, 1316
Hpy188I TCNGA 7 cut(s) 137, 376, 809, 886, 1389, 1624, 1731
Hpy8I GTNNAC 2 cut(s) 1000, 1316
Hpy99I CGWCG 4 cut(s) 148, 287, 299, 380
HpyAV CCTTC 2 cut(s) 328, 913
HpyCH4III ACNGT 7 cut(s) 49, 55, 392, 869, 1101, 1775, 1790
HpyCH4IV ACGT 3 cut(s) 285, 449, 970
HpyF10VI GCNNNNNNNGC 8 cut(s) 621, 1202, 1235, 1298, 1307, 1439, 1478, 1610
HpyF3I CTNAG 4 cut(s) 608, 1083, 1341, 1737
HpySE526I ACGT 3 cut(s) 285, 449, 970
Hsp92I GRCGYC 2 cut(s) 285, 297
HspAI GCGC 4 cut(s) 10, 108, 259, 1638
KpnI GGTACC 1 cut(s) 1349
KroI GCCGGC 1 cut(s) 359
KroNI GCCGGC 1 cut(s) 361
Kzo9I GATC 9 cut(s) 5, 183, 217, 409, 472, 886, 1020, 1412, 1765
LguI GCTCTTC 2 cut(s) 117, 149
LmnI GCTCC 3 cut(s) 1064, 1439, 1549
Lsp1109I GCAGC 4 cut(s) 171, 174, 539, 770
LweI GCATC 7 cut(s) 608, 1060, 1310, 1468, 1490, 1628, 1698
MaeI CTAG 2 cut(s) 1311, 1646
MaeII ACGT 3 cut(s) 285, 449, 970
MaeIII GTNAC 4 cut(s) 49, 392, 441, 766
MalI GATC 9 cut(s) 7, 185, 219, 411, 474, 888, 1022, 1414, 1767
MboI GATC 9 cut(s) 5, 183, 217, 409, 472, 886, 1020, 1412, 1765
MfeI CAATTG 2 cut(s) 762, 1695
MflI RGATCY 1 cut(s) 1765
MhlI GDGCHC 1 cut(s) 783
MluI ACGCGT 1 cut(s) 613
MlyI GAGTC 1 cut(s) 180
MmeI TCCRAC 2 cut(s) 399, 626
Mph1103I ATGCAT 4 cut(s) 470, 601, 1483, 1621
MreI CGCCGGCG 1 cut(s) 359
MroNI GCCGGC 1 cut(s) 359
MroXI GAANNNNTTC 1 cut(s) 260
MseI TTAA 8 cut(s) 549, 584, 626, 738, 795, 1103, 1154, 1545
MslI CAYNNNNRTG 3 cut(s) 56, 1292, 1592
MspA1I CMGCKG 2 cut(s) 658, 1418
MspI CCGG 4 cut(s) 180, 341, 360, 714
MspR9I CCNGG 5 cut(s) 15, 181, 715, 789, 1307
MssI GTTTAAAC 1 cut(s) 585
MunI CAATTG 2 cut(s) 762, 1695
Mva1269I GAATGC 2 cut(s) 470, 1660
MvaI CCWGG 3 cut(s) 15, 789, 1307
MvnI CGCG 5 cut(s) 10, 108, 201, 414, 615
MwoI GCNNNNNNNGC 8 cut(s) 621, 1202, 1235, 1298, 1307, 1439, 1478, 1610
NaeI GCCGGC 1 cut(s) 361
NciI CCSGG 2 cut(s) 181, 715
NcoI CCATGG 1 cut(s) 1243
NdeI CATATG 4 cut(s) 532, 631, 1328, 1703
NdeII GATC 9 cut(s) 5, 183, 217, 409, 472, 886, 1020, 1412, 1765
NgoMIV GCCGGC 1 cut(s) 359
NlaIV GGNNCC 5 cut(s) 979, 1066, 1347, 1767, 1787
NmuCI GTSAC 2 cut(s) 49, 441
NsiI ATGCAT 4 cut(s) 470, 601, 1483, 1621
NspI RCATGY 4 cut(s) 592, 774, 1061, 1608
PaeR7I CTCGAG 1 cut(s) 913
PciI ACATGT 1 cut(s) 588
PciSI GCTCTTC 2 cut(s) 117, 149
PcsI WCGNNNNNNNCGW 4 cut(s) 213, 291, 446, 1606
PctI GAATGC 2 cut(s) 470, 1660
PdiI GCCGGC 1 cut(s) 361
PdmI GAANNNNTTC 1 cut(s) 260
PfeI GAWTC 3 cut(s) 1284, 1597, 1732
PkrI GCNGC 6 cut(s) 24, 161, 164, 529, 760, 1091
Ple19I CGATCG 2 cut(s) 8, 412
PleI GAGTC 1 cut(s) 179
PmaCI CACGTG 1 cut(s) 971
PmeI GTTTAAAC 1 cut(s) 585
PmlI CACGTG 1 cut(s) 971
PpsI GAGTC 1 cut(s) 179
Ppu21I YACGTR 1 cut(s) 971
PscI ACATGT 1 cut(s) 588
Psp6I CCWGG 3 cut(s) 13, 787, 1305
PspCI CACGTG 1 cut(s) 971
PspFI CCCAGC 2 cut(s) 980, 1197
PspGI CCWGG 3 cut(s) 13, 787, 1305
PspN4I GGNNCC 5 cut(s) 979, 1066, 1347, 1767, 1787
PspPI GGNCC 3 cut(s) 716, 977, 1364
PsuI RGATCY 1 cut(s) 1765
PvuI CGATCG 2 cut(s) 8, 412
PvuII CAGCTG 2 cut(s) 658, 1418
RgaI GCGATCGC 1 cut(s) 8
RsaI GTAC 3 cut(s) 417, 1347, 1515
RsaNI GTAC 3 cut(s) 416, 1346, 1514
RseI CAYNNNNRTG 3 cut(s) 56, 1292, 1592
SapI GCTCTTC 2 cut(s) 117, 149
SaqAI TTAA 8 cut(s) 549, 584, 626, 738, 795, 1103, 1154, 1545
SatI GCNGC 6 cut(s) 23, 160, 163, 528, 759, 1090
Sau3AI GATC 9 cut(s) 5, 183, 217, 409, 472, 886, 1020, 1412, 1765
Sau96I GGNCC 3 cut(s) 716, 977, 1364
SchI GAGTC 1 cut(s) 180
ScrFI CCNGG 5 cut(s) 15, 181, 715, 789, 1307
SduI GDGCHC 1 cut(s) 783
SfaAI GCGATCGC 1 cut(s) 8
SfaNI GCATC 7 cut(s) 608, 1060, 1310, 1468, 1490, 1628, 1698
SfcI CTRYAG 2 cut(s) 1113, 1457
Sfr274I CTCGAG 1 cut(s) 913
SgfI GCGATCGC 1 cut(s) 8
SgrAI CRCCGGYG 1 cut(s) 359
SlaI CTCGAG 1 cut(s) 913
SmiMI CAYNNNNRTG 3 cut(s) 56, 1292, 1592
SmlI CTYRAG 4 cut(s) 326, 422, 577, 913
SmoI CTYRAG 4 cut(s) 326, 422, 577, 913
SsiI CCGC 5 cut(s) 23, 199, 363, 774, 1090
SspMI CTAG 2 cut(s) 1311, 1646
StyD4I CCNGG 5 cut(s) 13, 179, 713, 787, 1305
StyI CCWWGG 1 cut(s) 1243
TaaI ACNGT 7 cut(s) 49, 55, 392, 869, 1101, 1775, 1790
TaiI ACGT 3 cut(s) 288, 452, 973
TaqI TCGA 8 cut(s) 99, 169, 207, 252, 348, 914, 1176, 1777
TatI WGTACW 1 cut(s) 1513
TauI GCSGC 2 cut(s) 25, 1092
TfiI GAWTC 3 cut(s) 1284, 1597, 1732
Tru1I TTAA 8 cut(s) 549, 584, 626, 738, 795, 1103, 1154, 1545
Tru9I TTAA 8 cut(s) 549, 584, 626, 738, 795, 1103, 1154, 1545
TscAI CASTG 2 cut(s) 1087, 1285
TseFI GTSAC 2 cut(s) 49, 441
TseI GCWGC 4 cut(s) 159, 162, 527, 758
Tsp45I GTSAC 2 cut(s) 49, 441
TspDTI ATGAA 6 cut(s) 693, 695, 926, 1276, 1652, 1810
TspGWI ACGGA 2 cut(s) 72, 360
TspRI CASTG 2 cut(s) 1087, 1285
XapI RAATTY 5 cut(s) 552, 692, 1210, 1450, 1629
XceI RCATGY 4 cut(s) 592, 774, 1061, 1608
XhoI CTCGAG 1 cut(s) 913
XmnI GAANNNNTTC 1 cut(s) 260
XspI CTAG 2 cut(s) 1311, 1646
ZraI GACGTC 1 cut(s) 286
Zsp2I ATGCAT 4 cut(s) 470, 601, 1483, 1621
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.