Rh2BG242300

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0008632
Physical Location & Seq
Reverse (-)
24281400 .. 24287089
5690 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG242300.1

Sequence Viewer

Length: 1278 bp
ATGGGCACTCTCTCGCATGCGATCGCGCCCAGGCTCGCCGCCCTCCACCACCACCGCCGCCGTCACGGTAATGGAGTATTCTCCTCCTCCTCCGTCTCTTCTTCTTCTTCTCGAACCTCGCGCTCTTCTTCTTCTTCTTCTGCTTCGGGATTGACGACGAAGAAGAAGAGCAGCAGCGTTCGAGTCTTGGCCGGGATCAAGCACAACCACGGCGACCTCGATTTCAGCGATCCTGACTGGAAATCAAAGTTCCAGAGCGATTTCGAGAAGCGCTTCGCCATCCCTCACATCACCGACGTCTTCCCCGACGCCGTTCCCATTCCCTCCACCTTCTGCCTCAAGATGAGAACTCCGGTCATCGAAGACTTCGCCGGCGGATATCCCTCCGACGAGGAGTGGCACGGTTACATAAATAACAACGATCGCGTACTTCTCAAGACTATATACTACTCGTCACCTACGTCTGCTGGTGCCGAATGCATTGATCCTAACTGTACTTGGGTGGAGCAATGGGTTCATCGTGCTGGGCCACGAGAAAAGATATATTTTAAACCCGAAGAAGTGAAAGCAGCAATTGTAACATGCGGAGGGCTCTGTCCTGGTCTTAATGATGTCATCAGACAGATTGTCATTACACTTGAAATATATGGTGTAAAAAAGATTGTTGGTATTCCTTACGGTTATCGTGGCTTTTCTGATCCAGAATTAGCCGAAATGCCACTCTCAAGGAAGGTGGTTCAAAATGTTCATCTTTCTGGTGGAAGCTTGTTAGGAGTTTCACGTGGAGGCCCCAGCGTTAGTGAAATAGTGGACAATTTAGAGGAAAGAGGGATCAATATGCTTTTTGTTTTGGGTGGCAACGGCACACATGCTGGAGCTGATGCAATACACACTGAGTGCCGCAAAAGACAGTTAAGGGTGGCTATAGTTGGTGTGCCGAAAACCATAGACAATGACATTTTGTTAATGGACAAAACATTTGGTTTCGATACTGCTGTTGAAGAAGCCCAGCGAGCAATAAATTCAGCATATATTGAGGCACATAGTGCTTACCATGGTATTGGCGTTGTGAAATTGATGGGTCGTAGCAGTGGATTCATAGCAATGCACGCATCCCTGGCTAGTGGACAAATTGACATATGTTTGATACCTGAGGTACCTTTTCAAATACATGGCCCTCATGGTATTCTGCACCATCTGAAATACCTGATTGAAACAAAGGGATCAGCTGTGGTCTGTGTAGCAGAGGGAGCAGGGCAGGTAAGTTATAATTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003872 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008443 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009536 GO:0009987 GO:0016052 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019200 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044262 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046835 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0061615 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko00051 ko00052 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04152 ko05230 map00010 map00030 map00051 map00052 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04152 map05230
Pfam Domains
Protein Families
PFK

Protein Analysis

425

Amino Acids

46.14

Weight (kDa)

7.7

Isoelectric Point (pI)

42.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PFK PF00365 190 - 419 1.5e-56 Phosphofructokinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1269
AatII GACGTC 1 cut(s) 300
Acc36I ACCTGC 1 cut(s) 1249
Acc65I GGTACC 1 cut(s) 1156
AccB1I GGYRCC 2 cut(s) 470, 1156
AccII CGCG 3 cut(s) 26, 121, 426
AciI CCGC 6 cut(s) 39, 55, 58, 375, 585, 901
AclWI GGATC 6 cut(s) 203, 224, 479, 692, 839, 1231
AcoI YGGCCR 1 cut(s) 189
AcsI RAATTY 1 cut(s) 1021
AcvI CACGTG 1 cut(s) 782
AcyI GRCGYC 2 cut(s) 297, 309
AdeI CACNNNGTG 2 cut(s) 897, 1046
AfaI GTAC 3 cut(s) 429, 496, 1158
AfeI AGCGCT 1 cut(s) 272
AgsI TTSAA 5 cut(s) 641, 740, 1001, 1166, 1214
AhdI GACNNNNNGTC 1 cut(s) 626
AjnI CCWGG 3 cut(s) 29, 598, 1116
AluBI AGCT 3 cut(s) 765, 878, 1229
AluI AGCT 3 cut(s) 765, 878, 1229
Alw26I GTCTC 1 cut(s) 100
AlwI GGATC 6 cut(s) 203, 224, 479, 692, 839, 1231
Aor51HI AGCGCT 1 cut(s) 272
AoxI GGCC 4 cut(s) 189, 527, 787, 1174
ApeKI GCWGC 3 cut(s) 171, 174, 569
ApoI RAATTY 1 cut(s) 1021
ArsI GACNNNNNNTTYG 2 cut(s) 962, 994
AsiSI GCGATCGC 1 cut(s) 24
Asp700I GAANNNNTTC 1 cut(s) 272
Asp718I GGTACC 1 cut(s) 1156
AspLEI GCGC 3 cut(s) 28, 123, 273
AspS9I GGNCC 3 cut(s) 527, 788, 1175
AsuC2I CCSGG 1 cut(s) 193
AsuHPI GGTGA 2 cut(s) 283, 447
AxyI CCTNAGG 1 cut(s) 1152
BaeGI GKGCMC 1 cut(s) 8
BaeI ACNNNNGTAYC 2 cut(s) 981, 1014
BanI GGYRCC 2 cut(s) 470, 1156
BanII GRGCYC 1 cut(s) 594
BauI CACGAG 1 cut(s) 531
BbrPI CACGTG 1 cut(s) 782
BbsI GAAGAC 2 cut(s) 292, 369
BbvI GCAGC 3 cut(s) 183, 186, 581
BccI CCATC 3 cut(s) 287, 1072, 1203
BceAI ACGGC 4 cut(s) 45, 226, 296, 877
BciT130I CCWGG 3 cut(s) 31, 600, 1118
BcnI CCSGG 1 cut(s) 193
BcoDI GTCTC 1 cut(s) 100
BfaI CTAG 1 cut(s) 1122
BfmI CTRYAG 1 cut(s) 924
BfoI RGCGCY 1 cut(s) 274
BfuAI ACCTGC 1 cut(s) 1249
BisI GCNGC 6 cut(s) 39, 58, 172, 175, 570, 901
BlsI GCNGC 6 cut(s) 40, 59, 173, 176, 571, 902
Bme1390I CCNGG 4 cut(s) 31, 193, 600, 1118
BmeRI GACNNNNNGTC 1 cut(s) 626
BmgT120I GGNCC 3 cut(s) 527, 788, 1175
BmiI GGNNCC 3 cut(s) 472, 790, 1158
BmrFI CCNGG 4 cut(s) 31, 193, 600, 1118
BmsI GCATC 2 cut(s) 871, 1121
BpiI GAAGAC 2 cut(s) 292, 369
BpmI CTGGAG 1 cut(s) 894
BpuEI CTTGAG 3 cut(s) 323, 419, 709
BpuMI CCSGG 1 cut(s) 193
BsaAI YACGTR 1 cut(s) 782
BsaHI GRCGYC 2 cut(s) 297, 309
BsaJI CCNNGG 4 cut(s) 29, 208, 1054, 1116
BsaWI WCCGGW 1 cut(s) 352
Bse118I RCCGGY 1 cut(s) 371
Bse1I ACTGG 1 cut(s) 242
Bse21I CCTNAGG 1 cut(s) 1152
Bse3DI GCAATG 2 cut(s) 515, 1110
BseBI CCWGG 3 cut(s) 31, 600, 1118
BseDI CCNNGG 4 cut(s) 29, 208, 1054, 1116
BseGI GGATG 2 cut(s) 279, 1112
BseMI GCAATG 2 cut(s) 515, 1110
BseMII CTCAG 2 cut(s) 885, 1143
BseNI ACTGG 1 cut(s) 242
BseRI GAGGAG 4 cut(s) 73, 76, 79, 407
BseSI GKGCMC 1 cut(s) 8
BseXI GCAGC 3 cut(s) 183, 186, 581
BseYI CCCAGC 3 cut(s) 524, 791, 1008
BsgI GTGCAG 1 cut(s) 1175
Bsh1236I CGCG 3 cut(s) 26, 121, 426
Bsh1285I CGRYCG 2 cut(s) 24, 424
BshFI GGCC 4 cut(s) 191, 529, 789, 1176
BshNI GGYRCC 2 cut(s) 470, 1156
BsiEI CGRYCG 2 cut(s) 24, 424
BsiSI CCGG 3 cut(s) 192, 353, 372
BsmAI GTCTC 1 cut(s) 100
BsmBI CGTCTC 1 cut(s) 100
BsmI GAATGC 1 cut(s) 482
BsnI GGCC 4 cut(s) 191, 529, 789, 1176
Bsp1286I GDGCHC 2 cut(s) 8, 594
Bsp143I GATC 8 cut(s) 21, 195, 229, 421, 484, 697, 831, 1223
Bsp19I CCATGG 1 cut(s) 1054
BspACI CCGC 6 cut(s) 39, 55, 58, 375, 585, 901
BspANI GGCC 4 cut(s) 191, 529, 789, 1176
BspCNI CTCAG 2 cut(s) 886, 1144
BspFNI CGCG 3 cut(s) 26, 121, 426
BspLI GGNNCC 3 cut(s) 472, 790, 1158
BspMI ACCTGC 1 cut(s) 1249
BspPI GGATC 6 cut(s) 203, 224, 479, 692, 839, 1231
BspQI GCTCTTC 2 cut(s) 130, 161
BspT107I GGYRCC 2 cut(s) 470, 1156
BsrDI GCAATG 2 cut(s) 515, 1110
BsrFI RCCGGY 1 cut(s) 371
BsrI ACTGG 1 cut(s) 242
BssAI RCCGGY 1 cut(s) 371
BssECI CCNNGG 4 cut(s) 29, 208, 1054, 1116
BssMI GATC 8 cut(s) 21, 195, 229, 421, 484, 697, 831, 1223
BssNI GRCGYC 2 cut(s) 297, 309
BssSI CACGAG 1 cut(s) 531
BssT1I CCWWGG 1 cut(s) 1054
Bst2BI CACGAG 1 cut(s) 531
Bst2UI CCWGG 3 cut(s) 31, 600, 1118
Bst4CI ACNGT 5 cut(s) 68, 404, 494, 680, 912
Bst6I CTCTTC 3 cut(s) 103, 130, 161
BstACI GRCGYC 2 cut(s) 297, 309
BstAPI GCANNNNNTGC 1 cut(s) 1046
BstBAI YACGTR 1 cut(s) 782
BstC8I GCNNGC 5 cut(s) 18, 36, 373, 1014, 1110
BstDEI CTNAG 2 cut(s) 894, 1152
BstDSI CCRYGG 2 cut(s) 208, 1054
BstF5I GGATG 2 cut(s) 279, 1112
BstFNI CGCG 3 cut(s) 26, 121, 426
BstH2I RGCGCY 1 cut(s) 274
BstHHI GCGC 3 cut(s) 28, 123, 273
BstKTI GATC 8 cut(s) 24, 198, 232, 424, 487, 700, 834, 1226
BstMAI GTCTC 1 cut(s) 100
BstMBI GATC 8 cut(s) 21, 195, 229, 421, 484, 697, 831, 1223
BstMCI CGRYCG 2 cut(s) 24, 424
BstMWI GCNNNNNNNGC 5 cut(s) 1013, 1046, 1109, 1118, 1250
BstNI CCWGG 3 cut(s) 31, 600, 1118
BstNSI RCATGY 3 cut(s) 20, 585, 872
BstSCI CCNGG 4 cut(s) 29, 191, 598, 1116
BstSFI CTRYAG 1 cut(s) 924
BstSLI GKGCMC 1 cut(s) 8
BstUI CGCG 3 cut(s) 26, 121, 426
BstV1I GCAGC 3 cut(s) 183, 186, 581
BstV2I GAAGAC 2 cut(s) 292, 369
BstXI CCANNNNNNTGG 1 cut(s) 1061
Bsu36I CCTNAGG 1 cut(s) 1152
BsuRI GGCC 4 cut(s) 191, 529, 789, 1176
BtgI CCRYGG 2 cut(s) 208, 1054
BtsCI GGATG 2 cut(s) 279, 1112
BtsI GCAGTG 1 cut(s) 1096
BtsIMutI CAGTG 2 cut(s) 891, 1096
BveI ACCTGC 1 cut(s) 1249
Cac8I GCNNGC 5 cut(s) 18, 36, 373, 1014, 1110
CfoI GCGC 3 cut(s) 28, 123, 273
Cfr10I RCCGGY 1 cut(s) 371
Cfr13I GGNCC 3 cut(s) 527, 788, 1175
CseI GACGC 1 cut(s) 317
Csp6I GTAC 3 cut(s) 428, 495, 1157
CspCI CAANNNNNGTGG 2 cut(s) 714, 749
CviAII CATG 6 cut(s) 17, 582, 869, 1055, 1172, 1181
CviQI GTAC 3 cut(s) 428, 495, 1157
DdeI CTNAG 2 cut(s) 894, 1152
DpnI GATC 8 cut(s) 23, 197, 231, 423, 486, 699, 833, 1225
DpnII GATC 8 cut(s) 21, 195, 229, 421, 484, 697, 831, 1223
DraI TTTAAA 1 cut(s) 550
DraIII CACNNNGTG 2 cut(s) 897, 1046
DriI GACNNNNNGTC 1 cut(s) 626
EaeI YGGCCR 1 cut(s) 189
Eam1104I CTCTTC 3 cut(s) 103, 130, 161
Eam1105I GACNNNNNGTC 1 cut(s) 626
EarI CTCTTC 3 cut(s) 103, 130, 161
EciI GGCGGA 1 cut(s) 390
Eco130I CCWWGG 1 cut(s) 1054
Eco24I GRGCYC 1 cut(s) 594
Eco32I GATATC 1 cut(s) 380
Eco47III AGCGCT 1 cut(s) 272
Eco72I CACGTG 1 cut(s) 782
Eco81I CCTNAGG 1 cut(s) 1152
EcoO109I RGGNCCY 1 cut(s) 788
EcoRII CCWGG 3 cut(s) 29, 598, 1116
EcoRV GATATC 1 cut(s) 380
EcoT14I CCWWGG 1 cut(s) 1054
EcoT22I ATGCAT 1 cut(s) 482
EcoT38I GRGCYC 1 cut(s) 594
ErhI CCWWGG 1 cut(s) 1054
Esp3I CGTCTC 1 cut(s) 100
FaeI CATG 6 cut(s) 20, 585, 872, 1058, 1175, 1184
FatI CATG 6 cut(s) 16, 581, 868, 1054, 1171, 1180
FauNDI CATATG 1 cut(s) 1139
Fnu4HI GCNGC 6 cut(s) 39, 58, 172, 175, 570, 901
FokI GGATG 2 cut(s) 266, 1099
FriOI GRGCYC 1 cut(s) 594
Fsp4HI GCNGC 6 cut(s) 39, 58, 172, 175, 570, 901
FspBI CTAG 1 cut(s) 1122
GlaI GCGC 3 cut(s) 27, 122, 272
GluI GCNGC 6 cut(s) 39, 58, 172, 175, 570, 901
GsaI CCCAGC 3 cut(s) 528, 795, 1012
GsuI CTGGAG 1 cut(s) 894
HaeII RGCGCY 1 cut(s) 274
HaeIII GGCC 4 cut(s) 191, 529, 789, 1176
HapII CCGG 3 cut(s) 192, 353, 372
HgaI GACGC 1 cut(s) 317
HhaI GCGC 3 cut(s) 28, 123, 273
Hin1I GRCGYC 2 cut(s) 297, 309
Hin1II CATG 6 cut(s) 20, 585, 872, 1058, 1175, 1184
Hin6I GCGC 3 cut(s) 26, 121, 271
HinP1I GCGC 3 cut(s) 26, 121, 271
HindIII AAGCTT 1 cut(s) 763
HinfI GANTC 2 cut(s) 183, 1095
HpaII CCGG 3 cut(s) 192, 353, 372
HphI GGTGA 2 cut(s) 283, 447
Hpy166II GTNNAC 2 cut(s) 811, 1127
Hpy188I TCNGA 4 cut(s) 388, 620, 697, 1200
Hpy188III TCNNGA 8 cut(s) 111, 147, 233, 253, 265, 340, 436, 701
Hpy8I GTNNAC 2 cut(s) 811, 1127
Hpy99I CGWCG 4 cut(s) 160, 299, 311, 392
HpyAV CCTTC 2 cut(s) 340, 724
HpyCH4III ACNGT 5 cut(s) 68, 404, 494, 680, 912
HpyCH4IV ACGT 3 cut(s) 297, 461, 781
HpyCH4V TGCA 4 cut(s) 480, 884, 1108, 1192
HpyF10VI GCNNNNNNNGC 5 cut(s) 1013, 1046, 1109, 1118, 1250
HpyF3I CTNAG 2 cut(s) 894, 1152
HpySE526I ACGT 3 cut(s) 297, 461, 781
Hsp92I GRCGYC 2 cut(s) 297, 309
Hsp92II CATG 6 cut(s) 20, 585, 872, 1058, 1175, 1184
HspAI GCGC 3 cut(s) 26, 121, 271
KpnI GGTACC 1 cut(s) 1160
KroI GCCGGC 1 cut(s) 371
KroNI GCCGGC 1 cut(s) 373
Kzo9I GATC 8 cut(s) 21, 195, 229, 421, 484, 697, 831, 1223
LguI GCTCTTC 2 cut(s) 130, 161
LmnI GCTCC 3 cut(s) 505, 875, 1250
Lsp1109I GCAGC 3 cut(s) 183, 186, 581
LweI GCATC 2 cut(s) 871, 1121
MaeI CTAG 1 cut(s) 1122
MaeII ACGT 3 cut(s) 297, 461, 781
MaeIII GTNAC 4 cut(s) 62, 404, 453, 577
MalI GATC 8 cut(s) 23, 197, 231, 423, 486, 699, 833, 1225
MboI GATC 8 cut(s) 21, 195, 229, 421, 484, 697, 831, 1223
MfeI CAATTG 1 cut(s) 573
MhlI GDGCHC 2 cut(s) 8, 594
MluCI AATT 7 cut(s) 573, 704, 814, 1021, 1073, 1131, 1270
MlyI GAGTC 1 cut(s) 192
MmeI TCCRAC 1 cut(s) 411
Mph1103I ATGCAT 1 cut(s) 482
MreI CGCCGGCG 1 cut(s) 371
MroNI GCCGGC 1 cut(s) 371
MroXI GAANNNNTTC 1 cut(s) 272
MseI TTAA 4 cut(s) 549, 606, 914, 965
MslI CAYNNNNRTG 2 cut(s) 69, 1103
MspA1I CMGCKG 1 cut(s) 1229
MspI CCGG 3 cut(s) 192, 353, 372
MspR9I CCNGG 4 cut(s) 31, 193, 600, 1118
MunI CAATTG 1 cut(s) 573
Mva1269I GAATGC 1 cut(s) 482
MvaI CCWGG 3 cut(s) 31, 600, 1118
MvnI CGCG 3 cut(s) 26, 121, 426
MwoI GCNNNNNNNGC 5 cut(s) 1013, 1046, 1109, 1118, 1250
NaeI GCCGGC 1 cut(s) 373
NciI CCSGG 1 cut(s) 193
NcoI CCATGG 1 cut(s) 1054
NdeI CATATG 1 cut(s) 1139
NdeII GATC 8 cut(s) 21, 195, 229, 421, 484, 697, 831, 1223
NgoMIV GCCGGC 1 cut(s) 371
NlaIII CATG 6 cut(s) 20, 585, 872, 1058, 1175, 1184
NlaIV GGNNCC 3 cut(s) 472, 790, 1158
NmuCI GTSAC 2 cut(s) 62, 453
NsiI ATGCAT 1 cut(s) 482
NspI RCATGY 3 cut(s) 20, 585, 872
PaeI GCATGC 1 cut(s) 20
PciSI GCTCTTC 2 cut(s) 130, 161
PcsI WCGNNNNNNNCGW 5 cut(s) 152, 216, 225, 303, 458
PctI GAATGC 1 cut(s) 482
PdiI GCCGGC 1 cut(s) 373
PdmI GAANNNNTTC 1 cut(s) 272
PfeI GAWTC 1 cut(s) 1095
PkrI GCNGC 6 cut(s) 40, 59, 173, 176, 571, 902
Ple19I CGATCG 2 cut(s) 24, 424
PleI GAGTC 1 cut(s) 191
PmaCI CACGTG 1 cut(s) 782
PmlI CACGTG 1 cut(s) 782
PpsI GAGTC 1 cut(s) 191
Ppu21I YACGTR 1 cut(s) 782
PsiI TTATAA 1 cut(s) 1269
Psp6I CCWGG 3 cut(s) 29, 598, 1116
PspCI CACGTG 1 cut(s) 782
PspFI CCCAGC 3 cut(s) 524, 791, 1008
PspGI CCWGG 3 cut(s) 29, 598, 1116
PspN4I GGNNCC 3 cut(s) 472, 790, 1158
PspPI GGNCC 3 cut(s) 527, 788, 1175
PvuI CGATCG 2 cut(s) 24, 424
PvuII CAGCTG 1 cut(s) 1229
RgaI GCGATCGC 1 cut(s) 24
RsaI GTAC 3 cut(s) 429, 496, 1158
RsaNI GTAC 3 cut(s) 428, 495, 1157
RseI CAYNNNNRTG 2 cut(s) 69, 1103
SapI GCTCTTC 2 cut(s) 130, 161
SaqAI TTAA 4 cut(s) 549, 606, 914, 965
SatI GCNGC 6 cut(s) 39, 58, 172, 175, 570, 901
Sau3AI GATC 8 cut(s) 21, 195, 229, 421, 484, 697, 831, 1223
Sau96I GGNCC 3 cut(s) 527, 788, 1175
SchI GAGTC 1 cut(s) 192
ScrFI CCNGG 4 cut(s) 31, 193, 600, 1118
SduI GDGCHC 2 cut(s) 8, 594
SfaAI GCGATCGC 1 cut(s) 24
SfaNI GCATC 2 cut(s) 871, 1121
SfcI CTRYAG 1 cut(s) 924
SgfI GCGATCGC 1 cut(s) 24
SgrAI CRCCGGYG 1 cut(s) 371
SmiMI CAYNNNNRTG 2 cut(s) 69, 1103
SmlI CTYRAG 3 cut(s) 338, 434, 724
SmoI CTYRAG 3 cut(s) 338, 434, 724
SphI GCATGC 1 cut(s) 20
Sse9I AATT 7 cut(s) 573, 704, 814, 1021, 1073, 1131, 1270
SsiI CCGC 6 cut(s) 39, 55, 58, 375, 585, 901
SspMI CTAG 1 cut(s) 1122
StyD4I CCNGG 4 cut(s) 29, 191, 598, 1116
StyI CCWWGG 1 cut(s) 1054
TaaI ACNGT 5 cut(s) 68, 404, 494, 680, 912
TaiI ACGT 3 cut(s) 300, 464, 784
TaqI TCGA 6 cut(s) 112, 181, 219, 264, 360, 987
TasI AATT 7 cut(s) 573, 704, 814, 1021, 1073, 1131, 1270
TatI WGTACW 1 cut(s) 494
TauI GCSGC 3 cut(s) 41, 60, 903
TfiI GAWTC 1 cut(s) 1095
Tru1I TTAA 4 cut(s) 549, 606, 914, 965
Tru9I TTAA 4 cut(s) 549, 606, 914, 965
TscAI CASTG 2 cut(s) 898, 1096
TseFI GTSAC 2 cut(s) 62, 453
TseI GCWGC 3 cut(s) 171, 174, 569
Tsp45I GTSAC 2 cut(s) 62, 453
TspDTI ATGAA 3 cut(s) 506, 737, 1087
TspGWI ACGGA 1 cut(s) 82
TspRI CASTG 2 cut(s) 898, 1096
XapI RAATTY 1 cut(s) 1021
XceI RCATGY 3 cut(s) 20, 585, 872
XmnI GAANNNNTTC 1 cut(s) 272
XspI CTAG 1 cut(s) 1122
ZraI GACGTC 1 cut(s) 298
Zsp2I ATGCAT 1 cut(s) 482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.