Rorug02G0177100

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0008632
Physical Location & Seq
Forward (+)
16272656 .. 16273574
919 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0177100.1

Sequence Viewer

Length: 426 bp
ATGTATCTTCTCCAAAGCTGTGATTTACTGGAGCCTACAAAAGCAGGCATTGACAACGCATTTGAGGTTGTTTGCAGTTTATCCAGGGTTGAAGATCTTACTCTAAACCCAGAGACCATGAAGGCAAAGAAATTTATGGCTCTTCTTCGCGAAGGATCAAGGCCACCACCATTTGATAATGTTAGTAATCTGTACATCTGGGTTACTGTGAAGCTGCCCGATGGGTCTAAAGAAATTGAGTTAGCAAGGTATATGCTTGAGCATGCTCGAAATTTAACAATAATGGTCGTAATTTTTACGTCTCAACATTTGGAGAAAGTTACGATGAAGTTGAAGACTAGGAAGATGGTTTCCGGTGCTGCAGTTGTCTTTCAGGAATACAGATACCAAGTAACCAATTCCTATACTTTGTTTGTCAGAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003872 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008443 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009536 GO:0009987 GO:0016052 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019200 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044262 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046835 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0061615 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko00051 ko00052 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04152 ko05230 map00010 map00030 map00051 map00052 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04152 map05230
Pfam Domains
Protein Families
PFK

Protein Analysis

141

Amino Acids

16.22

Weight (kDa)

8.89

Isoelectric Point (pI)

38.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 150
AclWI GGATC 1 cut(s) 163
AcsI RAATTY 3 cut(s) 131, 271, 420
AfaI GTAC 1 cut(s) 194
AgsI TTSAA 2 cut(s) 92, 334
AjnI CCWGG 1 cut(s) 83
AluBI AGCT 2 cut(s) 18, 214
AluI AGCT 2 cut(s) 18, 214
Alw26I GTCTC 2 cut(s) 107, 306
AlwI GGATC 1 cut(s) 163
AoxI GGCC 1 cut(s) 161
ApeKI GCWGC 2 cut(s) 214, 359
ApoI RAATTY 3 cut(s) 131, 271, 420
ArsI GACNNNNNNTTYG 2 cut(s) 44, 76
BbsI GAAGAC 1 cut(s) 341
BbvI GCAGC 2 cut(s) 201, 346
BccI CCATC 2 cut(s) 215, 340
BciT130I CCWGG 1 cut(s) 85
BcoDI GTCTC 2 cut(s) 107, 306
BfaI CTAG 1 cut(s) 339
BfmI CTRYAG 1 cut(s) 360
BglII AGATCT 1 cut(s) 94
BisI GCNGC 2 cut(s) 215, 360
BlsI GCNGC 2 cut(s) 216, 361
Bme1390I CCNGG 1 cut(s) 85
BmiI GGNNCC 1 cut(s) 33
BmrFI CCNGG 1 cut(s) 85
BpiI GAAGAC 1 cut(s) 341
BpmI CTGGAG 1 cut(s) 50
BpuEI CTTGAG 1 cut(s) 278
BsaI GGTCTC 1 cut(s) 107
BsaJI CCNNGG 1 cut(s) 84
BsaWI WCCGGW 1 cut(s) 353
Bse1I ACTGG 1 cut(s) 33
BseBI CCWGG 1 cut(s) 85
BseDI CCNNGG 1 cut(s) 84
BseNI ACTGG 1 cut(s) 33
BseXI GCAGC 2 cut(s) 201, 346
Bsh1236I CGCG 1 cut(s) 150
BshFI GGCC 1 cut(s) 163
BsiSI CCGG 1 cut(s) 354
BsmAI GTCTC 2 cut(s) 107, 306
BsmBI CGTCTC 1 cut(s) 306
BsnI GGCC 1 cut(s) 163
Bso31I GGTCTC 1 cut(s) 107
Bsp1407I TGTACA 1 cut(s) 192
Bsp143I GATC 2 cut(s) 94, 155
Bsp68I TCGCGA 1 cut(s) 150
BspANI GGCC 1 cut(s) 163
BspFNI CGCG 1 cut(s) 150
BspLI GGNNCC 1 cut(s) 33
BspMAI CTGCAG 1 cut(s) 364
BspPI GGATC 1 cut(s) 163
BspQI GCTCTTC 1 cut(s) 147
BspTNI GGTCTC 1 cut(s) 107
BsrGI TGTACA 1 cut(s) 192
BsrI ACTGG 1 cut(s) 33
BssECI CCNNGG 1 cut(s) 84
BssMI GATC 2 cut(s) 94, 155
Bst2UI CCWGG 1 cut(s) 85
Bst4CI ACNGT 1 cut(s) 208
Bst6I CTCTTC 1 cut(s) 147
BstAUI TGTACA 1 cut(s) 192
BstC8I GCNNGC 2 cut(s) 46, 264
BstFNI CGCG 1 cut(s) 150
BstKTI GATC 2 cut(s) 97, 158
BstMAI GTCTC 2 cut(s) 107, 306
BstMBI GATC 2 cut(s) 94, 155
BstNI CCWGG 1 cut(s) 85
BstNSI RCATGY 1 cut(s) 266
BstSCI CCNGG 1 cut(s) 83
BstSFI CTRYAG 1 cut(s) 360
BstUI CGCG 1 cut(s) 150
BstV1I GCAGC 2 cut(s) 201, 346
BstV2I GAAGAC 1 cut(s) 341
BstX2I RGATCY 1 cut(s) 94
BstYI RGATCY 1 cut(s) 94
BsuRI GGCC 1 cut(s) 163
BtuMI TCGCGA 1 cut(s) 150
Cac8I GCNNGC 2 cut(s) 46, 264
Csp6I GTAC 1 cut(s) 193
CviAII CATG 2 cut(s) 118, 263
CviJI RGCY 5 cut(s) 18, 34, 140, 163, 214
CviKI_1 RGCY 5 cut(s) 18, 34, 140, 163, 214
CviQI GTAC 1 cut(s) 193
DpnI GATC 2 cut(s) 96, 157
DpnII GATC 2 cut(s) 94, 155
Eam1104I CTCTTC 1 cut(s) 147
EarI CTCTTC 1 cut(s) 147
Eco31I GGTCTC 1 cut(s) 107
EcoRII CCWGG 1 cut(s) 83
Esp3I CGTCTC 1 cut(s) 306
FaeI CATG 2 cut(s) 121, 266
FaiI YATR 6 cut(s) 119, 137, 252, 254, 264, 405
FatI CATG 2 cut(s) 117, 262
Fnu4HI GCNGC 2 cut(s) 215, 360
Fsp4HI GCNGC 2 cut(s) 215, 360
FspBI CTAG 1 cut(s) 339
GluI GCNGC 2 cut(s) 215, 360
GsuI CTGGAG 1 cut(s) 50
HaeIII GGCC 1 cut(s) 163
HapII CCGG 1 cut(s) 354
Hin1II CATG 2 cut(s) 121, 266
HpaII CCGG 1 cut(s) 354
Hpy188I TCNGA 1 cut(s) 419
Hpy188III TCNNGA 2 cut(s) 149, 374
HpyAV CCTTC 2 cut(s) 115, 146
HpyCH4III ACNGT 1 cut(s) 208
HpyCH4IV ACGT 1 cut(s) 299
HpyCH4V TGCA 2 cut(s) 75, 362
HpySE526I ACGT 1 cut(s) 299
Hsp92II CATG 2 cut(s) 121, 266
Kzo9I GATC 2 cut(s) 94, 155
LguI GCTCTTC 1 cut(s) 147
LmnI GCTCC 1 cut(s) 31
LpnPI CCDG 8 cut(s) 14, 30, 70, 97, 123, 184, 359, 367
Lsp1109I GCAGC 2 cut(s) 201, 346
MaeI CTAG 1 cut(s) 339
MaeII ACGT 1 cut(s) 299
MaeIII GTNAC 3 cut(s) 202, 319, 391
MalI GATC 2 cut(s) 96, 157
MboI GATC 2 cut(s) 94, 155
MboII GAAGA 5 cut(s) 104, 134, 137, 346, 355
MflI RGATCY 1 cut(s) 94
MluCI AATT 6 cut(s) 131, 234, 271, 291, 397, 420
MnlI CCTC 1 cut(s) 58
MseI TTAA 1 cut(s) 275
MspI CCGG 1 cut(s) 354
MspR9I CCNGG 1 cut(s) 85
MvaI CCWGG 1 cut(s) 85
MvnI CGCG 1 cut(s) 150
NdeII GATC 2 cut(s) 94, 155
NlaIII CATG 2 cut(s) 121, 266
NlaIV GGNNCC 1 cut(s) 33
NruI TCGCGA 1 cut(s) 150
NspI RCATGY 1 cut(s) 266
PaeI GCATGC 1 cut(s) 266
PciSI GCTCTTC 1 cut(s) 147
PkrI GCNGC 2 cut(s) 216, 361
Psp6I CCWGG 1 cut(s) 83
PspGI CCWGG 1 cut(s) 83
PspN4I GGNNCC 1 cut(s) 33
PstI CTGCAG 1 cut(s) 364
PsuI RGATCY 1 cut(s) 94
RruI TCGCGA 1 cut(s) 150
RsaI GTAC 1 cut(s) 194
RsaNI GTAC 1 cut(s) 193
SapI GCTCTTC 1 cut(s) 147
SaqAI TTAA 1 cut(s) 275
SatI GCNGC 2 cut(s) 215, 360
Sau3AI GATC 2 cut(s) 94, 155
ScrFI CCNGG 1 cut(s) 85
SetI ASST 5 cut(s) 20, 69, 216, 251, 302
SfcI CTRYAG 1 cut(s) 360
SmlI CTYRAG 1 cut(s) 257
SmoI CTYRAG 1 cut(s) 257
SphI GCATGC 1 cut(s) 266
Sse9I AATT 6 cut(s) 131, 234, 271, 291, 397, 420
SspMI CTAG 1 cut(s) 339
StyD4I CCNGG 1 cut(s) 83
TaaI ACNGT 1 cut(s) 208
TaiI ACGT 1 cut(s) 302
TaqI TCGA 1 cut(s) 268
TasI AATT 6 cut(s) 131, 234, 271, 291, 397, 420
TatI WGTACW 1 cut(s) 192
Tru1I TTAA 1 cut(s) 275
Tru9I TTAA 1 cut(s) 275
TseI GCWGC 2 cut(s) 214, 359
TspDTI ATGAA 2 cut(s) 134, 341
XapI RAATTY 3 cut(s) 131, 271, 420
XceI RCATGY 1 cut(s) 266
XspI CTAG 1 cut(s) 339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.