RLG00000017938

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0008632
Physical Location & Seq
Reverse (-)
23252115 .. 23256753
4639 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017938

Sequence Viewer

Length: 1638 bp
ATGGCCACTCTGTCGCATGCGATCGCGCCCAGGCCTGCCGCCCTCCACCACCACGGCCGTCACGGTAATGGAGTATTCTCCTCCTCCTCCGTCTCTTCTTCTTCTCGAAGCTCGCGCTCTTCTTCTTCTTCTTCTTCTTCTGCTTCGGGATTGACGACGAAGAAGAAGAGCAGCAGCGTTCGAGTCTTGGCCGGGATCAAGCACGACCACCGCGACCTTGATTTCAGCGATCCTGACTGGAAATCAAAGTTCCAGAGCGATTTCGAGAAGCGCTTCGCCATCCCTCACATCACCGACGTCTTCCCCGACGCCGTTCCCATTCCCTCCACCTTCTGCCTCAAGATGAGAACTCCGGTCATCGAAGACTTCGCCGGCGGATATCCCTCCGACGAGGAGTGGCACGGTTACATAAATAACAACGATCGCGTACTTCTCAAGACTATATACTACTCGTCACCTACGTCTGCTGGTGCCGAATGCATTGATCCTAACTGTACTTGGGTGGAGCAATGGGTTCATCGTGCCGGGCCACGAGAAAAGATATATTTTAAACCCGAAGAAGTGAAAGCAGCAATTGTAACATGCGGAGGGCTCTGTCCTGGTCTTAATGATGTCATCAGACAGATTGTCATCACACTTGAAATATATGGTGTAAAAAAGATTGTTGGTATTCCTTACGGTTATCGTGGCTTTTCTGATCCAGAATTAGCCGAAATGCCACTCTCAAGGAAGGTGGTTCAAAATGTTCATCTTTCTGGTGGAAGCTTGTTAGGAGTTTCACGTGGAGGCCCCAGCGTTAGTGAAATAGTGGACAATTTAGAGGAAAGAGGGATCAATATGCTCTTTGTTTTGGGTGGAAACGGCACACATGCTGGAGCCGACGCAATACACACTGAGTGCCGCAAAAGACAGTTAAGGGTGGCTATAGTTGGTGTGCCGAAAACCATAGACAATGACATTTTGTTAATGGACAAAACATTTGGTTTCGATACTGCTGTTGAAGAAGCCCAGCGAGCAATAAATTCAGCATATATTGAGGCACATAGTGCTTACCATGGTATTGGCATTGTGAAATTGATGGGTCGTAGCAGTGGATTCATAGCAATGCACGCATCCCTGGCTAGTGGACAAATTGACATATGTTTGATACCTGAGGTACCTTTTCAAATACATGGCCCTCATGGTATTCTGCACCATCTGAAATACCTGATTGAAACAAAGGGATCAGCTGTGGTCTGTGTAGCAGAGGGAGCAGGGCAGAATTTACTACAGAAAACCAATGCTACTGATGCATCAGGAAATGTTGTATTTGGGGATATTGGAGTACATATTCAACAACAGACAAAGAAACATTTTAAGGAGCATGGTGTTCCTGTTGATGTGAAATATATAGACCCCACATACATGATTCGTGCATGTCGTGCAAATGCATCAGATGGAATTCTTTGCGCTGTGCTAGGGCAGAATGCTGTTCATGGTGCATTTGCTGGATATAGTGGCATCACAATTGGCATATGCAACACTCATTATGTATATCTTCCGATTCCTGAGGTTATTTCTCATCCCCGACTGGTGGATCCCAACAGTCGAATGTGGCACCGTTGCTTGACTTCAACAGGCCAGCCAGACTTCATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003872 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008443 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009536 GO:0009987 GO:0016052 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019200 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044262 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046835 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0061615 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko00051 ko00052 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04152 ko05230 map00010 map00030 map00051 map00052 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04152 map05230
Pfam Domains
Protein Families
PFK

Protein Analysis

546

Amino Acids

59.15

Weight (kDa)

7.38

Isoelectric Point (pI)

39.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PFK PF00365 190 - 492 8.7e-62 Phosphofructokinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 300
Acc65I GGTACC 1 cut(s) 1156
AccB1I GGYRCC 3 cut(s) 470, 1156, 1596
AccII CGCG 4 cut(s) 26, 115, 213, 426
AciI CCGC 5 cut(s) 39, 211, 375, 585, 901
AclWI GGATC 8 cut(s) 203, 224, 479, 692, 839, 1231, 1571, 1584
AcoI YGGCCR 3 cut(s) 3, 55, 189
AcsI RAATTY 3 cut(s) 1021, 1261, 1440
AcvI CACGTG 1 cut(s) 782
AcyI GRCGYC 2 cut(s) 297, 309
AdeI CACNNNGTG 2 cut(s) 897, 1046
AfaI GTAC 4 cut(s) 429, 496, 1158, 1326
AfeI AGCGCT 1 cut(s) 272
AfiI CCNNNNNNNGG 1 cut(s) 1573
AgsI TTSAA 7 cut(s) 641, 740, 1001, 1166, 1214, 1334, 1614
AhdI GACNNNNNGTC 1 cut(s) 626
AjnI CCWGG 3 cut(s) 29, 598, 1116
AluBI AGCT 3 cut(s) 111, 765, 1229
AluI AGCT 3 cut(s) 111, 765, 1229
Alw26I GTCTC 1 cut(s) 97
AlwI GGATC 8 cut(s) 203, 224, 479, 692, 839, 1231, 1571, 1584
Aor51HI AGCGCT 1 cut(s) 272
AoxI GGCC 8 cut(s) 3, 32, 55, 189, 527, 787, 1174, 1618
ApeKI GCWGC 3 cut(s) 171, 174, 569
ApoI RAATTY 3 cut(s) 1021, 1261, 1440
ArsI GACNNNNNNTTYG 2 cut(s) 962, 994
AsiSI GCGATCGC 1 cut(s) 24
Asp700I GAANNNNTTC 1 cut(s) 272
Asp718I GGTACC 1 cut(s) 1156
AspLEI GCGC 4 cut(s) 28, 117, 273, 1451
AspS9I GGNCC 3 cut(s) 527, 788, 1175
AsuC2I CCSGG 2 cut(s) 193, 526
AsuHPI GGTGA 2 cut(s) 283, 447
AxyI CCTNAGG 2 cut(s) 1152, 1548
BaeI ACNNNNGTAYC 2 cut(s) 981, 1014
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 1576
BanI GGYRCC 3 cut(s) 470, 1156, 1596
BanII GRGCYC 1 cut(s) 594
BauI CACGAG 1 cut(s) 531
BbrPI CACGTG 1 cut(s) 782
BbsI GAAGAC 2 cut(s) 292, 369
BbvI GCAGC 3 cut(s) 183, 186, 581
BccI CCATC 4 cut(s) 287, 1072, 1203, 1430
BceAI ACGGC 4 cut(s) 42, 70, 296, 877
BciT130I CCWGG 3 cut(s) 31, 600, 1118
BcnI CCSGG 2 cut(s) 193, 526
BcoDI GTCTC 1 cut(s) 97
BfaI CTAG 2 cut(s) 1122, 1457
BfmI CTRYAG 2 cut(s) 924, 1268
BfoI RGCGCY 1 cut(s) 274
BisI GCNGC 5 cut(s) 39, 172, 175, 570, 901
BlsI GCNGC 5 cut(s) 40, 173, 176, 571, 902
Bme1390I CCNGG 5 cut(s) 31, 193, 526, 600, 1118
BmeRI GACNNNNNGTC 1 cut(s) 626
BmgT120I GGNCC 3 cut(s) 527, 788, 1175
BmiI GGNNCC 6 cut(s) 472, 790, 877, 1158, 1578, 1598
BmrFI CCNGG 5 cut(s) 31, 193, 526, 600, 1118
BmsI GCATC 5 cut(s) 1121, 1279, 1301, 1439, 1509
BpiI GAAGAC 2 cut(s) 292, 369
BpmI CTGGAG 1 cut(s) 894
BpuEI CTTGAG 3 cut(s) 323, 419, 709
BpuMI CCSGG 2 cut(s) 193, 526
BsaAI YACGTR 1 cut(s) 782
BsaBI GATNNNNATC 1 cut(s) 629
BsaHI GRCGYC 2 cut(s) 297, 309
BsaJI CCNNGG 4 cut(s) 29, 52, 1054, 1116
BsaWI WCCGGW 1 cut(s) 352
Bsc4I CCNNNNNNNGG 1 cut(s) 1573
Bse118I RCCGGY 1 cut(s) 371
Bse1I ACTGG 2 cut(s) 242, 1575
Bse21I CCTNAGG 2 cut(s) 1152, 1548
Bse3DI GCAATG 2 cut(s) 515, 1110
Bse8I GATNNNNATC 1 cut(s) 629
BseBI CCWGG 3 cut(s) 31, 600, 1118
BseDI CCNNGG 4 cut(s) 29, 52, 1054, 1116
BseGI GGATG 3 cut(s) 279, 1112, 1561
BseJI GATNNNNATC 1 cut(s) 629
BseLI CCNNNNNNNGG 1 cut(s) 1573
BseMI GCAATG 2 cut(s) 515, 1110
BseMII CTCAG 3 cut(s) 885, 1143, 1539
BseNI ACTGG 2 cut(s) 242, 1575
BseRI GAGGAG 4 cut(s) 70, 73, 76, 407
BseX3I CGGCCG 1 cut(s) 55
BseXI GCAGC 3 cut(s) 183, 186, 581
BseYI CCCAGC 2 cut(s) 791, 1008
BsgI GTGCAG 1 cut(s) 1175
Bsh1236I CGCG 4 cut(s) 26, 115, 213, 426
Bsh1285I CGRYCG 3 cut(s) 24, 58, 424
BshFI GGCC 8 cut(s) 5, 34, 57, 191, 529, 789, 1176, 1620
BshNI GGYRCC 3 cut(s) 470, 1156, 1596
BsiEI CGRYCG 3 cut(s) 24, 58, 424
BsiSI CCGG 4 cut(s) 192, 353, 372, 525
BslI CCNNNNNNNGG 1 cut(s) 1573
BsmAI GTCTC 1 cut(s) 97
BsmBI CGTCTC 1 cut(s) 97
BsmI GAATGC 2 cut(s) 482, 1471
BsnI GGCC 8 cut(s) 5, 34, 57, 191, 529, 789, 1176, 1620
Bsp1286I GDGCHC 1 cut(s) 594
Bsp143I GATC 9 cut(s) 21, 195, 229, 421, 484, 697, 831, 1223, 1576
Bsp19I CCATGG 1 cut(s) 1054
BspACI CCGC 5 cut(s) 39, 211, 375, 585, 901
BspANI GGCC 8 cut(s) 5, 34, 57, 191, 529, 789, 1176, 1620
BspCNI CTCAG 3 cut(s) 886, 1144, 1540
BspFNI CGCG 4 cut(s) 26, 115, 213, 426
BspLI GGNNCC 6 cut(s) 472, 790, 877, 1158, 1578, 1598
BspPI GGATC 8 cut(s) 203, 224, 479, 692, 839, 1231, 1571, 1584
BspQI GCTCTTC 2 cut(s) 124, 161
BspT107I GGYRCC 3 cut(s) 470, 1156, 1596
BsrDI GCAATG 2 cut(s) 515, 1110
BsrFI RCCGGY 1 cut(s) 371
BsrI ACTGG 2 cut(s) 242, 1575
BssAI RCCGGY 1 cut(s) 371
BssECI CCNNGG 4 cut(s) 29, 52, 1054, 1116
BssMI GATC 9 cut(s) 21, 195, 229, 421, 484, 697, 831, 1223, 1576
BssNI GRCGYC 2 cut(s) 297, 309
BssSI CACGAG 1 cut(s) 531
BssT1I CCWWGG 1 cut(s) 1054
Bst2BI CACGAG 1 cut(s) 531
Bst2UI CCWGG 3 cut(s) 31, 600, 1118
Bst4CI ACNGT 7 cut(s) 65, 404, 494, 680, 912, 1586, 1601
Bst6I CTCTTC 3 cut(s) 100, 124, 161
BstACI GRCGYC 2 cut(s) 297, 309
BstAPI GCANNNNNTGC 2 cut(s) 1046, 1421
BstBAI YACGTR 1 cut(s) 782
BstC8I GCNNGC 7 cut(s) 18, 36, 113, 373, 1014, 1110, 1622
BstDEI CTNAG 3 cut(s) 894, 1152, 1548
BstDSI CCRYGG 2 cut(s) 52, 1054
BstF5I GGATG 3 cut(s) 279, 1112, 1561
BstFNI CGCG 4 cut(s) 26, 115, 213, 426
BstH2I RGCGCY 1 cut(s) 274
BstHHI GCGC 4 cut(s) 28, 117, 273, 1451
BstKTI GATC 9 cut(s) 24, 198, 232, 424, 487, 700, 834, 1226, 1579
BstMAI GTCTC 1 cut(s) 97
BstMBI GATC 9 cut(s) 21, 195, 229, 421, 484, 697, 831, 1223, 1576
BstMCI CGRYCG 3 cut(s) 24, 58, 424
BstMWI GCNNNNNNNGC 7 cut(s) 1013, 1046, 1109, 1118, 1250, 1289, 1421
BstNI CCWGG 3 cut(s) 31, 600, 1118
BstNSI RCATGY 4 cut(s) 20, 585, 872, 1419
BstSCI CCNGG 5 cut(s) 29, 191, 524, 598, 1116
BstSFI CTRYAG 2 cut(s) 924, 1268
BstUI CGCG 4 cut(s) 26, 115, 213, 426
BstV1I GCAGC 3 cut(s) 183, 186, 581
BstV2I GAAGAC 2 cut(s) 292, 369
BstX2I RGATCY 1 cut(s) 1576
BstXI CCANNNNNNTGG 1 cut(s) 1061
BstYI RGATCY 1 cut(s) 1576
BstZI CGGCCG 1 cut(s) 55
Bsu36I CCTNAGG 2 cut(s) 1152, 1548
BsuRI GGCC 8 cut(s) 5, 34, 57, 191, 529, 789, 1176, 1620
BtgI CCRYGG 2 cut(s) 52, 1054
BtsCI GGATG 3 cut(s) 279, 1112, 1561
BtsI GCAGTG 1 cut(s) 1096
BtsIMutI CAGTG 2 cut(s) 891, 1096
Cac8I GCNNGC 7 cut(s) 18, 36, 113, 373, 1014, 1110, 1622
CfoI GCGC 4 cut(s) 28, 117, 273, 1451
Cfr10I RCCGGY 1 cut(s) 371
Cfr13I GGNCC 3 cut(s) 527, 788, 1175
CseI GACGC 2 cut(s) 317, 890
Csp6I GTAC 4 cut(s) 428, 495, 1157, 1325
CspCI CAANNNNNGTGG 2 cut(s) 714, 749
CviQI GTAC 4 cut(s) 428, 495, 1157, 1325
DdeI CTNAG 3 cut(s) 894, 1152, 1548
DpnI GATC 9 cut(s) 23, 197, 231, 423, 486, 699, 833, 1225, 1578
DpnII GATC 9 cut(s) 21, 195, 229, 421, 484, 697, 831, 1223, 1576
DraI TTTAAA 1 cut(s) 550
DraIII CACNNNGTG 2 cut(s) 897, 1046
DriI GACNNNNNGTC 1 cut(s) 626
EaeI YGGCCR 3 cut(s) 3, 55, 189
EagI CGGCCG 1 cut(s) 55
Eam1104I CTCTTC 3 cut(s) 100, 124, 161
Eam1105I GACNNNNNGTC 1 cut(s) 626
EarI CTCTTC 3 cut(s) 100, 124, 161
EciI GGCGGA 1 cut(s) 390
EclXI CGGCCG 1 cut(s) 55
Eco130I CCWWGG 1 cut(s) 1054
Eco147I AGGCCT 1 cut(s) 34
Eco24I GRGCYC 1 cut(s) 594
Eco32I GATATC 1 cut(s) 380
Eco47III AGCGCT 1 cut(s) 272
Eco52I CGGCCG 1 cut(s) 55
Eco72I CACGTG 1 cut(s) 782
Eco81I CCTNAGG 2 cut(s) 1152, 1548
EcoO109I RGGNCCY 1 cut(s) 788
EcoRI GAATTC 1 cut(s) 1440
EcoRII CCWGG 3 cut(s) 29, 598, 1116
EcoRV GATATC 1 cut(s) 380
EcoT14I CCWWGG 1 cut(s) 1054
EcoT22I ATGCAT 3 cut(s) 482, 1294, 1432
EcoT38I GRGCYC 1 cut(s) 594
ErhI CCWWGG 1 cut(s) 1054
Esp3I CGTCTC 1 cut(s) 97
FauNDI CATATG 2 cut(s) 1139, 1514
Fnu4HI GCNGC 5 cut(s) 39, 172, 175, 570, 901
FokI GGATG 3 cut(s) 266, 1099, 1548
FriOI GRGCYC 1 cut(s) 594
Fsp4HI GCNGC 5 cut(s) 39, 172, 175, 570, 901
FspBI CTAG 2 cut(s) 1122, 1457
GlaI GCGC 4 cut(s) 27, 116, 272, 1450
GluI GCNGC 5 cut(s) 39, 172, 175, 570, 901
GsaI CCCAGC 2 cut(s) 795, 1012
GsuI CTGGAG 1 cut(s) 894
HaeII RGCGCY 1 cut(s) 274
HaeIII GGCC 8 cut(s) 5, 34, 57, 191, 529, 789, 1176, 1620
HapII CCGG 4 cut(s) 192, 353, 372, 525
HgaI GACGC 2 cut(s) 317, 890
HhaI GCGC 4 cut(s) 28, 117, 273, 1451
Hin1I GRCGYC 2 cut(s) 297, 309
Hin6I GCGC 4 cut(s) 26, 115, 271, 1449
HinP1I GCGC 4 cut(s) 26, 115, 271, 1449
HindIII AAGCTT 1 cut(s) 763
HinfI GANTC 4 cut(s) 183, 1095, 1408, 1543
HpaII CCGG 4 cut(s) 192, 353, 372, 525
HphI GGTGA 2 cut(s) 283, 447
Hpy166II GTNNAC 2 cut(s) 811, 1127
Hpy188I TCNGA 6 cut(s) 388, 620, 697, 1200, 1435, 1542
Hpy8I GTNNAC 2 cut(s) 811, 1127
Hpy99I CGWCG 5 cut(s) 160, 299, 311, 392, 884
HpyAV CCTTC 2 cut(s) 340, 724
HpyCH4III ACNGT 7 cut(s) 65, 404, 494, 680, 912, 1586, 1601
HpyCH4IV ACGT 3 cut(s) 297, 461, 781
HpyCH4V TGCA 9 cut(s) 480, 1108, 1192, 1292, 1415, 1424, 1430, 1481, 1518
HpyF10VI GCNNNNNNNGC 7 cut(s) 1013, 1046, 1109, 1118, 1250, 1289, 1421
HpyF3I CTNAG 3 cut(s) 894, 1152, 1548
HpySE526I ACGT 3 cut(s) 297, 461, 781
Hsp92I GRCGYC 2 cut(s) 297, 309
HspAI GCGC 4 cut(s) 26, 115, 271, 1449
KpnI GGTACC 1 cut(s) 1160
KroI GCCGGC 1 cut(s) 371
KroNI GCCGGC 1 cut(s) 373
Kzo9I GATC 9 cut(s) 21, 195, 229, 421, 484, 697, 831, 1223, 1576
LguI GCTCTTC 2 cut(s) 124, 161
LmnI GCTCC 4 cut(s) 505, 875, 1250, 1360
Lsp1109I GCAGC 3 cut(s) 183, 186, 581
LweI GCATC 5 cut(s) 1121, 1279, 1301, 1439, 1509
MaeI CTAG 2 cut(s) 1122, 1457
MaeII ACGT 3 cut(s) 297, 461, 781
MaeIII GTNAC 4 cut(s) 59, 404, 453, 577
MalI GATC 9 cut(s) 23, 197, 231, 423, 486, 699, 833, 1225, 1578
MboI GATC 9 cut(s) 21, 195, 229, 421, 484, 697, 831, 1223, 1576
MfeI CAATTG 2 cut(s) 573, 1506
MflI RGATCY 1 cut(s) 1576
MhlI GDGCHC 1 cut(s) 594
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 9 cut(s) 573, 704, 814, 1021, 1073, 1131, 1261, 1440, 1506
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 192
MmeI TCCRAC 1 cut(s) 411
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 3 cut(s) 482, 1294, 1432
MreI CGCCGGCG 1 cut(s) 371
MroNI GCCGGC 1 cut(s) 371
MroXI GAANNNNTTC 1 cut(s) 272
MscI TGGCCA 1 cut(s) 5
MseI TTAA 5 cut(s) 549, 606, 914, 965, 1356
MslI CAYNNNNRTG 3 cut(s) 66, 1103, 1403
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 1229
MspI CCGG 4 cut(s) 192, 353, 372, 525
MspR9I CCNGG 5 cut(s) 31, 193, 526, 600, 1118
MunI CAATTG 2 cut(s) 573, 1506
Mva1269I GAATGC 2 cut(s) 482, 1471
MvaI CCWGG 3 cut(s) 31, 600, 1118
MvnI CGCG 4 cut(s) 26, 115, 213, 426
MwoI GCNNNNNNNGC 7 cut(s) 1013, 1046, 1109, 1118, 1250, 1289, 1421
NaeI GCCGGC 1 cut(s) 373
NciI CCSGG 2 cut(s) 193, 526
NcoI CCATGG 1 cut(s) 1054
NdeI CATATG 2 cut(s) 1139, 1514
NdeII GATC 9 cut(s) 21, 195, 229, 421, 484, 697, 831, 1223, 1576
NgoMIV GCCGGC 1 cut(s) 371
NlaIV GGNNCC 6 cut(s) 472, 790, 877, 1158, 1578, 1598
NmuCI GTSAC 2 cut(s) 59, 453
NsiI ATGCAT 3 cut(s) 482, 1294, 1432
NspI RCATGY 4 cut(s) 20, 585, 872, 1419
PaeI GCATGC 1 cut(s) 20
PceI AGGCCT 1 cut(s) 34
PciSI GCTCTTC 2 cut(s) 124, 161
PcsI WCGNNNNNNNCGW 5 cut(s) 152, 210, 303, 458, 1417
PctI GAATGC 2 cut(s) 482, 1471
PdiI GCCGGC 1 cut(s) 373
PdmI GAANNNNTTC 1 cut(s) 272
PfeI GAWTC 3 cut(s) 1095, 1408, 1543
PkrI GCNGC 5 cut(s) 40, 173, 176, 571, 902
Ple19I CGATCG 2 cut(s) 24, 424
PleI GAGTC 1 cut(s) 191
PmaCI CACGTG 1 cut(s) 782
PmlI CACGTG 1 cut(s) 782
PpsI GAGTC 1 cut(s) 191
Ppu21I YACGTR 1 cut(s) 782
Psp6I CCWGG 3 cut(s) 29, 598, 1116
PspCI CACGTG 1 cut(s) 782
PspFI CCCAGC 2 cut(s) 791, 1008
PspGI CCWGG 3 cut(s) 29, 598, 1116
PspN4I GGNNCC 6 cut(s) 472, 790, 877, 1158, 1578, 1598
PspPI GGNCC 3 cut(s) 527, 788, 1175
PsuI RGATCY 1 cut(s) 1576
PvuI CGATCG 2 cut(s) 24, 424
PvuII CAGCTG 1 cut(s) 1229
RgaI GCGATCGC 1 cut(s) 24
RsaI GTAC 4 cut(s) 429, 496, 1158, 1326
RsaNI GTAC 4 cut(s) 428, 495, 1157, 1325
RseI CAYNNNNRTG 3 cut(s) 66, 1103, 1403
SapI GCTCTTC 2 cut(s) 124, 161
SaqAI TTAA 5 cut(s) 549, 606, 914, 965, 1356
SatI GCNGC 5 cut(s) 39, 172, 175, 570, 901
Sau3AI GATC 9 cut(s) 21, 195, 229, 421, 484, 697, 831, 1223, 1576
Sau96I GGNCC 3 cut(s) 527, 788, 1175
SchI GAGTC 1 cut(s) 192
ScrFI CCNGG 5 cut(s) 31, 193, 526, 600, 1118
SduI GDGCHC 1 cut(s) 594
SfaAI GCGATCGC 1 cut(s) 24
SfaNI GCATC 5 cut(s) 1121, 1279, 1301, 1439, 1509
SfcI CTRYAG 2 cut(s) 924, 1268
SgfI GCGATCGC 1 cut(s) 24
SgrAI CRCCGGYG 1 cut(s) 371
SmiMI CAYNNNNRTG 3 cut(s) 66, 1103, 1403
SmlI CTYRAG 3 cut(s) 338, 434, 724
SmoI CTYRAG 3 cut(s) 338, 434, 724
SphI GCATGC 1 cut(s) 20
Sse9I AATT 9 cut(s) 573, 704, 814, 1021, 1073, 1131, 1261, 1440, 1506
SseBI AGGCCT 1 cut(s) 34
SsiI CCGC 5 cut(s) 39, 211, 375, 585, 901
SspMI CTAG 2 cut(s) 1122, 1457
StuI AGGCCT 1 cut(s) 34
StyD4I CCNGG 5 cut(s) 29, 191, 524, 598, 1116
StyI CCWWGG 1 cut(s) 1054
TaaI ACNGT 7 cut(s) 65, 404, 494, 680, 912, 1586, 1601
TaiI ACGT 3 cut(s) 300, 464, 784
TaqI TCGA 6 cut(s) 106, 181, 264, 360, 987, 1588
TasI AATT 9 cut(s) 573, 704, 814, 1021, 1073, 1131, 1261, 1440, 1506
TatI WGTACW 2 cut(s) 494, 1324
TauI GCSGC 2 cut(s) 41, 903
TfiI GAWTC 3 cut(s) 1095, 1408, 1543
Tru1I TTAA 5 cut(s) 549, 606, 914, 965, 1356
Tru9I TTAA 5 cut(s) 549, 606, 914, 965, 1356
TscAI CASTG 2 cut(s) 898, 1096
TseFI GTSAC 2 cut(s) 59, 453
TseI GCWGC 3 cut(s) 171, 174, 569
Tsp45I GTSAC 2 cut(s) 59, 453
TspDTI ATGAA 5 cut(s) 506, 737, 1087, 1463, 1621
TspGWI ACGGA 1 cut(s) 79
TspRI CASTG 2 cut(s) 898, 1096
XapI RAATTY 3 cut(s) 1021, 1261, 1440
XceI RCATGY 4 cut(s) 20, 585, 872, 1419
XmnI GAANNNNTTC 1 cut(s) 272
XspI CTAG 2 cut(s) 1122, 1457
ZraI GACGTC 1 cut(s) 298
Zsp2I ATGCAT 3 cut(s) 482, 1294, 1432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.