MD01G1037400.v1.1

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0008632
Physical Location & Seq
Forward (+)
13000725 .. 13006694
5970 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1037400.v1.1.491

Sequence Viewer

Length: 1587 bp
ATGGCCACTCTCTCCCACGCGATCGCCCCGCCGGGACTCGGCCTCCACCGCCGCGCTTCTCACGGATTCTCTCCGCCGTCTCTGAGACTACTGACTACTTCCAGTGGAGTCACCAAGAACAGCAGCCGCGTCTTCAATCAGAGCAAGAGCCATAGCCGGCAGCAGATCGATTTCAGTGATCCCGACTGGAAATCCAAGTTCCAGAAGGATTTCGAGAAGCGGTTTAACATCCCTCACATCACTGATGTCTTCCCTGACGCCGTTCCCATTCCTTCCACCTTCTGCTTACAAATGAGGACTCCGGTGATTGAAGACTTTGCAGGTGGGTACCCGTCGGACGAAGAGTGGCACGGCTACATAAATAACAATGACCGGGTACTCCTTAAGACCATATACTACTCGTCGCATACGTCTGCTGGTGCTGAATGCATTGATCCTAATTGTACTTGGGTGGAGCAATGGGTTCATCGTGCTGGGCCTCGGGAAAAAATATATTTTAAACCAGAAGAAGTGAAAGCAGCAATTGTAACATGCGGAGGGCTCTGCCCTGGTCTTAATGACGTCATCAGACAGATCGTCATTACACTTGAAATATACGGTGTCAAACAGATTGTGGGGATTCCTTATGGTTACCGCGGGTTTTCTAACAATGAATTAGCTGAAATGCCACTATCTAGGAAAGTGGTTCAAAATGTTCATCTTTCTGGTGGAAGCTTGTTAGGAGTTTCACGTGGAGGTCCCAGTGTTAGTGACATTGTGGACAGTTTGGAGGAAAGAGGGATCAACATGGTTTTTGTTTTGGGTGGAAACGGCACACATGCTGGAGCCGATGCAATACACAATGAGTGCCGTAAAAGACAGTTAAGAGTGGCTGTAGTTGGTGTGCCGAAAACCATAGACAATGATATTTTGCTGATGGACAAAACTTTTGGTTTCGATACAGCTGTTGAAGAAGCTCAACGGGCAATAAATTCAGCATACATTGAGGCACATAGTGCTTACCATGGCGTTGGCATTGTTAAATTGATGGGTCGTAGCAGTGGATTCATAGCAATGCATGCATCCCTGGCTAGTGGACAAATTGACATATGTTTGATACCTGAGGTACCTTTTCAAATACATGGCCCTCATGGTATTTTGCACCATCTAAAATACCTAATTCAAACAAAGGGATCAGCTGTGGTCTGCGTAGCAGAGGGAGCAGGGCAGAATTTAATAGAGAAAACCAATGCTACTGATGCATCTGGAAATATGGTATATGGGGATATTGGCGTACATATTCAGCAAGAGACAAAAAAGTATTTTAAAGAGGCTGGTGTTCCCGTTGACGTGAAGTATATAGACCCCACATACATGATCCGTGCGTGTCGTGCAAATGCATCAGACGGAATACTTTGCGCTGTATTAGGGCAAAATGCTGTTCACGGGGCGTTCGCTGGATACAGTGGAATCACAGTTGGCACTTGCAACACTCATTATGTATACCTTCCAATTCCTGAGGTCATATCTCATCCCAGGCTGGTGGATCCGAACAGCCGAATGTGGCATCGTTGCTTGACTTCCACAGGTCAGCCGGACTTTATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003872 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008443 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009536 GO:0009987 GO:0016052 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019200 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044262 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046835 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0061615 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko00051 ko00052 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04152 ko05230 map00010 map00030 map00051 map00052 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04152 map05230
Pfam Domains
Protein Families
PFK

Protein Analysis

529

Amino Acids

57.69

Weight (kDa)

6.9

Isoelectric Point (pI)

36.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PFK PF00365 173 - 475 6.7e-61 Phosphofructokinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 311
AatII GACGTC 1 cut(s) 564
Acc36I ACCTGC 1 cut(s) 311
Acc65I GGTACC 2 cut(s) 327, 1105
AccB1I GGYRCC 2 cut(s) 327, 1105
AccI GTMKAC 1 cut(s) 1482
AccII CGCG 4 cut(s) 20, 54, 129, 636
AciI CCGC 9 cut(s) 29, 49, 52, 74, 127, 220, 534, 634, 636
AclWI GGATC 7 cut(s) 173, 428, 788, 1180, 1351, 1520, 1533
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 970, 1210
AcvI CACGTG 1 cut(s) 731
AcyI GRCGYC 2 cut(s) 258, 561
AdeI CACNNNGTG 1 cut(s) 995
AfaI GTAC 5 cut(s) 329, 378, 445, 1107, 1275
AfiI CCNNNNNNNGG 1 cut(s) 38
AflII CTTAAG 1 cut(s) 383
AgsI TTSAA 7 cut(s) 136, 311, 590, 689, 950, 1115, 1163
AhdI GACNNNNNGTC 1 cut(s) 575
AjiI CACGTC 1 cut(s) 1330
AjnI CCWGG 3 cut(s) 547, 1065, 1514
AluBI AGCT 5 cut(s) 659, 714, 944, 956, 1178
AluI AGCT 5 cut(s) 659, 714, 944, 956, 1178
Alw26I GTCTC 3 cut(s) 79, 84, 1283
AlwI GGATC 7 cut(s) 173, 428, 788, 1180, 1351, 1520, 1533
Ama87I CYCGRG 1 cut(s) 480
AoxI GGCC 4 cut(s) 3, 40, 476, 1123
ApeKI GCWGC 3 cut(s) 123, 160, 518
ApoI RAATTY 2 cut(s) 970, 1210
ArsI GACNNNNNNTTYG 2 cut(s) 911, 943
AsiSI GCGATCGC 1 cut(s) 24
Asp700I GAANNNNTTC 1 cut(s) 209
Asp718I GGTACC 2 cut(s) 327, 1105
AspLEI GCGC 2 cut(s) 56, 1400
AspS9I GGNCC 3 cut(s) 476, 737, 1124
AsuC2I CCSGG 2 cut(s) 33, 374
AsuHPI GGTGA 2 cut(s) 103, 316
AvaI CYCGRG 1 cut(s) 480
AvaII GGWCC 1 cut(s) 737
AxyI CCTNAGG 2 cut(s) 1101, 1497
BaeI ACNNNNGTAYC 2 cut(s) 930, 963
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 1525
BanI GGYRCC 2 cut(s) 327, 1105
BanII GRGCYC 1 cut(s) 543
BbrPI CACGTG 1 cut(s) 731
BbsI GAAGAC 3 cut(s) 124, 241, 318
BbvI GCAGC 3 cut(s) 135, 172, 530
BccI CCATC 3 cut(s) 910, 1021, 1152
BceAI ACGGC 5 cut(s) 61, 245, 367, 826, 834
BciT130I CCWGG 3 cut(s) 549, 1067, 1516
BciVI GTATCC 1 cut(s) 1433
BcnI CCSGG 2 cut(s) 33, 374
BcoDI GTCTC 3 cut(s) 79, 84, 1283
BfaI CTAG 3 cut(s) 675, 1071, 1585
BfmI CTRYAG 1 cut(s) 873
BfrI CTTAAG 1 cut(s) 383
BfuAI ACCTGC 1 cut(s) 311
BfuI GTATCC 1 cut(s) 1433
BisI GCNGC 5 cut(s) 52, 124, 127, 161, 519
BlsI GCNGC 5 cut(s) 53, 125, 128, 162, 520
Bme1390I CCNGG 5 cut(s) 33, 374, 549, 1067, 1516
Bme18I GGWCC 1 cut(s) 737
BmeRI GACNNNNNGTC 1 cut(s) 575
BmeT110I CYCGRG 1 cut(s) 480
BmgBI CACGTC 1 cut(s) 1330
BmgT120I GGNCC 3 cut(s) 476, 737, 1124
BmiI GGNNCC 5 cut(s) 329, 739, 826, 1107, 1527
BmrFI CCNGG 5 cut(s) 33, 374, 549, 1067, 1516
BmrI ACTGGG 1 cut(s) 735
BmsI GCATC 6 cut(s) 820, 1070, 1228, 1250, 1388, 1555
BmuI ACTGGG 1 cut(s) 735
BpiI GAAGAC 3 cut(s) 124, 241, 318
BpmI CTGGAG 1 cut(s) 843
BpuMI CCSGG 2 cut(s) 33, 374
Bsa29I ATCGAT 1 cut(s) 168
BsaAI YACGTR 1 cut(s) 731
BsaHI GRCGYC 2 cut(s) 258, 561
BsaJI CCNNGG 6 cut(s) 479, 547, 634, 1003, 1065, 1514
BsaWI WCCGGW 1 cut(s) 301
BsaXI ACNNNNNCTCC 2 cut(s) 27, 57
Bsc4I CCNNNNNNNGG 1 cut(s) 38
Bse118I RCCGGY 1 cut(s) 156
Bse1I ACTGG 3 cut(s) 102, 191, 741
Bse21I CCTNAGG 2 cut(s) 1101, 1497
Bse3DI GCAATG 2 cut(s) 464, 1059
BseBI CCWGG 3 cut(s) 549, 1067, 1516
BseCI ATCGAT 1 cut(s) 168
BseDI CCNNGG 6 cut(s) 479, 547, 634, 1003, 1065, 1514
BseGI GGATG 3 cut(s) 228, 1061, 1510
BseLI CCNNNNNNNGG 1 cut(s) 38
BseMI GCAATG 2 cut(s) 464, 1059
BseMII CTCAG 3 cut(s) 74, 1092, 1488
BseNI ACTGG 3 cut(s) 102, 191, 741
BseXI GCAGC 3 cut(s) 135, 172, 530
BseYI CCCAGC 1 cut(s) 473
Bsh1236I CGCG 4 cut(s) 20, 54, 129, 636
Bsh1285I CGRYCG 1 cut(s) 24
BshFI GGCC 4 cut(s) 5, 42, 478, 1125
BshNI GGYRCC 2 cut(s) 327, 1105
BshVI ATCGAT 1 cut(s) 168
BsiEI CGRYCG 1 cut(s) 24
BsiHKCI CYCGRG 1 cut(s) 480
BsiSI CCGG 5 cut(s) 32, 157, 302, 373, 1574
BslFI GGGAC 2 cut(s) 48, 723
BslI CCNNNNNNNGG 1 cut(s) 38
BsmAI GTCTC 3 cut(s) 79, 84, 1283
BsmBI CGTCTC 1 cut(s) 84
BsmFI GGGAC 2 cut(s) 48, 723
BsmI GAATGC 1 cut(s) 431
BsnI GGCC 4 cut(s) 5, 42, 478, 1125
BsoBI CYCGRG 1 cut(s) 480
Bsp1286I GDGCHC 1 cut(s) 543
Bsp143I GATC 9 cut(s) 21, 165, 178, 433, 573, 780, 1172, 1356, 1525
Bsp19I CCATGG 1 cut(s) 1003
BspACI CCGC 9 cut(s) 29, 49, 52, 74, 127, 220, 534, 634, 636
BspANI GGCC 4 cut(s) 5, 42, 478, 1125
BspCNI CTCAG 3 cut(s) 75, 1093, 1489
BspDI ATCGAT 1 cut(s) 168
BspFNI CGCG 4 cut(s) 20, 54, 129, 636
BspLI GGNNCC 5 cut(s) 329, 739, 826, 1107, 1527
BspMI ACCTGC 1 cut(s) 311
BspPI GGATC 7 cut(s) 173, 428, 788, 1180, 1351, 1520, 1533
BspT107I GGYRCC 2 cut(s) 327, 1105
BspTI CTTAAG 1 cut(s) 383
BsrDI GCAATG 2 cut(s) 464, 1059
BsrFI RCCGGY 1 cut(s) 156
BsrI ACTGG 3 cut(s) 102, 191, 741
BssAI RCCGGY 1 cut(s) 156
BssECI CCNNGG 6 cut(s) 479, 547, 634, 1003, 1065, 1514
BssMI GATC 9 cut(s) 21, 165, 178, 433, 573, 780, 1172, 1356, 1525
BssNAI GTATAC 1 cut(s) 1483
BssNI GRCGYC 2 cut(s) 258, 561
BssT1I CCWWGG 1 cut(s) 1003
Bst1107I GTATAC 1 cut(s) 1483
Bst2UI CCWGG 3 cut(s) 549, 1067, 1516
Bst4CI ACNGT 5 cut(s) 599, 764, 861, 1445, 1456
Bst6I CTCTTC 1 cut(s) 336
BstACI GRCGYC 2 cut(s) 258, 561
BstAFI CTTAAG 1 cut(s) 383
BstAPI GCANNNNNTGC 2 cut(s) 995, 1058
BstBAI YACGTR 1 cut(s) 731
BstC8I GCNNGC 2 cut(s) 158, 1059
BstDEI CTNAG 3 cut(s) 83, 1101, 1497
BstDSI CCRYGG 2 cut(s) 634, 1003
BstEII GGTNACC 1 cut(s) 629
BstF5I GGATG 3 cut(s) 228, 1061, 1510
BstFNI CGCG 4 cut(s) 20, 54, 129, 636
BstHHI GCGC 2 cut(s) 56, 1400
BstKTI GATC 9 cut(s) 24, 168, 181, 436, 576, 783, 1175, 1359, 1528
BstMAI GTCTC 3 cut(s) 79, 84, 1283
BstMBI GATC 9 cut(s) 21, 165, 178, 433, 573, 780, 1172, 1356, 1525
BstMCI CGRYCG 1 cut(s) 24
BstMWI GCNNNNNNNGC 8 cut(s) 48, 962, 995, 1058, 1067, 1199, 1238, 1370
BstNI CCWGG 3 cut(s) 549, 1067, 1516
BstNSI RCATGY 3 cut(s) 534, 821, 1061
BstPI GGTNACC 1 cut(s) 629
BstSCI CCNGG 5 cut(s) 31, 372, 547, 1065, 1514
BstSFI CTRYAG 1 cut(s) 873
BstUI CGCG 4 cut(s) 20, 54, 129, 636
BstV1I GCAGC 3 cut(s) 135, 172, 530
BstV2I GAAGAC 3 cut(s) 124, 241, 318
BstX2I RGATCY 1 cut(s) 1525
BstXI CCANNNNNNTGG 2 cut(s) 1010, 1522
BstYI RGATCY 1 cut(s) 1525
BstZ17I GTATAC 1 cut(s) 1483
Bsu15I ATCGAT 1 cut(s) 168
Bsu36I CCTNAGG 2 cut(s) 1101, 1497
BsuI GTATCC 1 cut(s) 1433
BsuRI GGCC 4 cut(s) 5, 42, 478, 1125
BsuTUI ATCGAT 1 cut(s) 168
BtgI CCRYGG 2 cut(s) 634, 1003
BtrI CACGTC 1 cut(s) 1330
BtsCI GGATG 3 cut(s) 228, 1061, 1510
BtsI GCAGTG 1 cut(s) 1045
BtsIMutI CAGTG 6 cut(s) 109, 181, 240, 748, 1045, 1450
BveI ACCTGC 1 cut(s) 311
Cac8I GCNNGC 2 cut(s) 158, 1059
CfoI GCGC 2 cut(s) 56, 1400
Cfr10I RCCGGY 1 cut(s) 156
Cfr13I GGNCC 3 cut(s) 476, 737, 1124
Cfr42I CCGCGG 1 cut(s) 637
ClaI ATCGAT 1 cut(s) 168
CseI GACGC 2 cut(s) 118, 266
Csp6I GTAC 5 cut(s) 328, 377, 444, 1106, 1274
CviAII CATG 8 cut(s) 531, 787, 818, 1004, 1058, 1121, 1130, 1354
CviQI GTAC 5 cut(s) 328, 377, 444, 1106, 1274
DdeI CTNAG 3 cut(s) 83, 1101, 1497
DpnI GATC 9 cut(s) 23, 167, 180, 435, 575, 782, 1174, 1358, 1527
DpnII GATC 9 cut(s) 21, 165, 178, 433, 573, 780, 1172, 1356, 1525
DraI TTTAAA 2 cut(s) 499, 1306
DraIII CACNNNGTG 1 cut(s) 995
DriI GACNNNNNGTC 1 cut(s) 575
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 336
Eam1105I GACNNNNNGTC 1 cut(s) 575
EarI CTCTTC 1 cut(s) 336
EciI GGCGGA 1 cut(s) 63
Eco130I CCWWGG 1 cut(s) 1003
Eco24I GRGCYC 1 cut(s) 543
Eco47I GGWCC 1 cut(s) 737
Eco72I CACGTG 1 cut(s) 731
Eco81I CCTNAGG 2 cut(s) 1101, 1497
Eco88I CYCGRG 1 cut(s) 480
Eco91I GGTNACC 1 cut(s) 629
EcoO109I RGGNCCY 1 cut(s) 737
EcoO65I GGTNACC 1 cut(s) 629
EcoRII CCWGG 3 cut(s) 547, 1065, 1514
EcoT14I CCWWGG 1 cut(s) 1003
EcoT22I ATGCAT 5 cut(s) 431, 1059, 1063, 1243, 1381
EcoT38I GRGCYC 1 cut(s) 543
ErhI CCWWGG 1 cut(s) 1003
Esp3I CGTCTC 1 cut(s) 84
FaeI CATG 8 cut(s) 534, 790, 821, 1007, 1061, 1124, 1133, 1357
FaqI GGGAC 2 cut(s) 48, 723
FatI CATG 8 cut(s) 530, 786, 817, 1003, 1057, 1120, 1129, 1353
FauI CCCGC 2 cut(s) 36, 629
FauNDI CATATG 1 cut(s) 1088
FblI GTMKAC 1 cut(s) 1482
Fnu4HI GCNGC 5 cut(s) 52, 124, 127, 161, 519
FokI GGATG 3 cut(s) 215, 1048, 1497
FriOI GRGCYC 1 cut(s) 543
Fsp4HI GCNGC 5 cut(s) 52, 124, 127, 161, 519
FspBI CTAG 3 cut(s) 675, 1071, 1585
GlaI GCGC 2 cut(s) 55, 1399
GluI GCNGC 5 cut(s) 52, 124, 127, 161, 519
GsaI CCCAGC 1 cut(s) 477
GsuI CTGGAG 1 cut(s) 843
HaeIII GGCC 4 cut(s) 5, 42, 478, 1125
HapII CCGG 5 cut(s) 32, 157, 302, 373, 1574
HgaI GACGC 2 cut(s) 118, 266
HhaI GCGC 2 cut(s) 56, 1400
Hin1I GRCGYC 2 cut(s) 258, 561
Hin1II CATG 8 cut(s) 534, 790, 821, 1007, 1061, 1124, 1133, 1357
Hin6I GCGC 2 cut(s) 54, 1398
HinP1I GCGC 2 cut(s) 54, 1398
HincII GTYRAC 1 cut(s) 1327
HindII GTYRAC 1 cut(s) 1327
HindIII AAGCTT 1 cut(s) 712
HinfI GANTC 7 cut(s) 36, 66, 108, 298, 619, 1044, 1449
HpaII CCGG 5 cut(s) 32, 157, 302, 373, 1574
HphI GGTGA 2 cut(s) 103, 316
Hpy166II GTNNAC 5 cut(s) 760, 1076, 1327, 1423, 1483
Hpy188I TCNGA 6 cut(s) 84, 141, 337, 569, 1384, 1530
Hpy188III TCNNGA 6 cut(s) 182, 202, 214, 482, 1245, 1496
Hpy8I GTNNAC 5 cut(s) 760, 1076, 1327, 1423, 1483
Hpy99I CGWCG 2 cut(s) 337, 406
HpyAV CCTTC 4 cut(s) 199, 282, 289, 1496
HpyCH4III ACNGT 5 cut(s) 599, 764, 861, 1445, 1456
HpyCH4IV ACGT 4 cut(s) 410, 561, 730, 1329
HpyF10VI GCNNNNNNNGC 8 cut(s) 48, 962, 995, 1058, 1067, 1199, 1238, 1370
HpyF3I CTNAG 3 cut(s) 83, 1101, 1497
HpySE526I ACGT 4 cut(s) 410, 561, 730, 1329
Hsp92I GRCGYC 2 cut(s) 258, 561
Hsp92II CATG 8 cut(s) 534, 790, 821, 1007, 1061, 1124, 1133, 1357
HspAI GCGC 2 cut(s) 54, 1398
KpnI GGTACC 2 cut(s) 331, 1109
KroI GCCGGC 1 cut(s) 156
KroNI GCCGGC 1 cut(s) 158
KspI CCGCGG 1 cut(s) 637
Kzo9I GATC 9 cut(s) 21, 165, 178, 433, 573, 780, 1172, 1356, 1525
LmnI GCTCC 3 cut(s) 454, 824, 1199
Lsp1109I GCAGC 3 cut(s) 135, 172, 530
LweI GCATC 6 cut(s) 820, 1070, 1228, 1250, 1388, 1555
MaeI CTAG 3 cut(s) 675, 1071, 1585
MaeII ACGT 4 cut(s) 410, 561, 730, 1329
MaeIII GTNAC 4 cut(s) 109, 526, 629, 749
MalI GATC 9 cut(s) 23, 167, 180, 435, 575, 782, 1174, 1358, 1527
MboI GATC 9 cut(s) 21, 165, 178, 433, 573, 780, 1172, 1356, 1525
MboII GAAGA 6 cut(s) 124, 241, 323, 353, 518, 962
MfeI CAATTG 1 cut(s) 522
MflI RGATCY 1 cut(s) 1525
MhlI GDGCHC 1 cut(s) 543
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 9 cut(s) 439, 522, 653, 970, 1022, 1080, 1158, 1210, 1491
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 3 cut(s) 30, 117, 292
MmeI TCCRAC 1 cut(s) 315
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 5 cut(s) 431, 1059, 1063, 1243, 1381
MroNI GCCGGC 1 cut(s) 156
MroXI GAANNNNTTC 1 cut(s) 209
MscI TGGCCA 1 cut(s) 5
MseI TTAA 8 cut(s) 225, 384, 498, 555, 863, 1020, 1214, 1305
MslI CAYNNNNRTG 2 cut(s) 1052, 1352
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 3 cut(s) 636, 944, 1178
MspCI CTTAAG 1 cut(s) 383
MspI CCGG 5 cut(s) 32, 157, 302, 373, 1574
MspR9I CCNGG 5 cut(s) 33, 374, 549, 1067, 1516
MunI CAATTG 1 cut(s) 522
Mva1269I GAATGC 1 cut(s) 431
MvaI CCWGG 3 cut(s) 549, 1067, 1516
MvnI CGCG 4 cut(s) 20, 54, 129, 636
MwoI GCNNNNNNNGC 8 cut(s) 48, 962, 995, 1058, 1067, 1199, 1238, 1370
NaeI GCCGGC 1 cut(s) 158
NciI CCSGG 2 cut(s) 33, 374
NcoI CCATGG 1 cut(s) 1003
NdeI CATATG 1 cut(s) 1088
NdeII GATC 9 cut(s) 21, 165, 178, 433, 573, 780, 1172, 1356, 1525
NgoMIV GCCGGC 1 cut(s) 156
NlaIII CATG 8 cut(s) 534, 790, 821, 1007, 1061, 1124, 1133, 1357
NlaIV GGNNCC 5 cut(s) 329, 739, 826, 1107, 1527
NmeAIII GCCGAG 1 cut(s) 18
NmuCI GTSAC 2 cut(s) 109, 749
NsiI ATGCAT 5 cut(s) 431, 1059, 1063, 1243, 1381
NspI RCATGY 3 cut(s) 534, 821, 1061
PaeI GCATGC 1 cut(s) 1061
PaqCI CACCTGC 1 cut(s) 311
PcsI WCGNNNNNNNCGW 1 cut(s) 407
PctI GAATGC 1 cut(s) 431
PdiI GCCGGC 1 cut(s) 158
PdmI GAANNNNTTC 1 cut(s) 209
PfeI GAWTC 4 cut(s) 66, 619, 1044, 1449
PkrI GCNGC 5 cut(s) 53, 125, 128, 162, 520
Ple19I CGATCG 1 cut(s) 24
PleI GAGTC 3 cut(s) 30, 116, 292
PmaCI CACGTG 1 cut(s) 731
PmlI CACGTG 1 cut(s) 731
PpsI GAGTC 3 cut(s) 30, 116, 292
Ppu21I YACGTR 1 cut(s) 731
PpuMI RGGWCCY 1 cut(s) 737
Psp5II RGGWCCY 1 cut(s) 737
Psp6I CCWGG 3 cut(s) 547, 1065, 1514
PspCI CACGTG 1 cut(s) 731
PspEI GGTNACC 1 cut(s) 629
PspFI CCCAGC 1 cut(s) 473
PspGI CCWGG 3 cut(s) 547, 1065, 1514
PspN4I GGNNCC 5 cut(s) 329, 739, 826, 1107, 1527
PspPI GGNCC 3 cut(s) 476, 737, 1124
PspPPI RGGWCCY 1 cut(s) 737
PsuI RGATCY 1 cut(s) 1525
PvuI CGATCG 1 cut(s) 24
PvuII CAGCTG 2 cut(s) 944, 1178
RgaI GCGATCGC 1 cut(s) 24
RsaI GTAC 5 cut(s) 329, 378, 445, 1107, 1275
RsaNI GTAC 5 cut(s) 328, 377, 444, 1106, 1274
RseI CAYNNNNRTG 2 cut(s) 1052, 1352
SacII CCGCGG 1 cut(s) 637
SaqAI TTAA 8 cut(s) 225, 384, 498, 555, 863, 1020, 1214, 1305
SatI GCNGC 5 cut(s) 52, 124, 127, 161, 519
Sau3AI GATC 9 cut(s) 21, 165, 178, 433, 573, 780, 1172, 1356, 1525
Sau96I GGNCC 3 cut(s) 476, 737, 1124
SchI GAGTC 3 cut(s) 30, 117, 292
ScrFI CCNGG 5 cut(s) 33, 374, 549, 1067, 1516
SduI GDGCHC 1 cut(s) 543
SfaAI GCGATCGC 1 cut(s) 24
SfaNI GCATC 6 cut(s) 820, 1070, 1228, 1250, 1388, 1555
SfcI CTRYAG 1 cut(s) 873
Sfr303I CCGCGG 1 cut(s) 637
SgfI GCGATCGC 1 cut(s) 24
SgrBI CCGCGG 1 cut(s) 637
SinI GGWCC 1 cut(s) 737
SmiMI CAYNNNNRTG 2 cut(s) 1052, 1352
SmlI CTYRAG 1 cut(s) 383
SmoI CTYRAG 1 cut(s) 383
SphI GCATGC 1 cut(s) 1061
Sse9I AATT 9 cut(s) 439, 522, 653, 970, 1022, 1080, 1158, 1210, 1491
SsiI CCGC 9 cut(s) 29, 49, 52, 74, 127, 220, 534, 634, 636
SspMI CTAG 3 cut(s) 675, 1071, 1585
StyD4I CCNGG 5 cut(s) 31, 372, 547, 1065, 1514
StyI CCWWGG 1 cut(s) 1003
TaaI ACNGT 5 cut(s) 599, 764, 861, 1445, 1456
TaiI ACGT 4 cut(s) 413, 564, 733, 1332
TaqI TCGA 3 cut(s) 168, 213, 936
TasI AATT 9 cut(s) 439, 522, 653, 970, 1022, 1080, 1158, 1210, 1491
TatI WGTACW 1 cut(s) 443
TauI GCSGC 2 cut(s) 54, 129
TfiI GAWTC 4 cut(s) 66, 619, 1044, 1449
Tru1I TTAA 8 cut(s) 225, 384, 498, 555, 863, 1020, 1214, 1305
Tru9I TTAA 8 cut(s) 225, 384, 498, 555, 863, 1020, 1214, 1305
TscAI CASTG 6 cut(s) 109, 181, 247, 748, 1045, 1450
TseFI GTSAC 2 cut(s) 109, 749
TseI GCWGC 3 cut(s) 123, 160, 518
Tsp45I GTSAC 2 cut(s) 109, 749
TspDTI ATGAA 4 cut(s) 455, 666, 686, 1036
TspGWI ACGGA 3 cut(s) 78, 1349, 1401
TspRI CASTG 6 cut(s) 109, 181, 247, 748, 1045, 1450
Vha464I CTTAAG 1 cut(s) 383
VpaK11BI GGWCC 1 cut(s) 737
XapI RAATTY 2 cut(s) 970, 1210
XceI RCATGY 3 cut(s) 534, 821, 1061
XmiI GTMKAC 1 cut(s) 1482
XmnI GAANNNNTTC 1 cut(s) 209
XspI CTAG 3 cut(s) 675, 1071, 1585
ZraI GACGTC 1 cut(s) 562
Zsp2I ATGCAT 5 cut(s) 431, 1059, 1063, 1243, 1381
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.