Rh2DG237200

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0008632
Physical Location & Seq
Reverse (-)
23743475 .. 23749311
5837 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG237200.1

Sequence Viewer

Length: 1272 bp
ATGGGCACTCTCTCGCATGCGATCGCGCCCAGGCTCGCCGCCCTCCACCACCACCGCCGCCGTCACGGTAATGGAGTATTCTCCTCCTCCTCCGTCTCTTCTTCTTCTCGAACCTCGCGCTCTTCTTCTTCTTCTGCTTCGGGATTGACGACGAAGAAGAAGAGCAGCAGCGTTCGAGTCTTGGCAGGGATCAAGCACAACCACGGCGACCTCGATTTCAGCGATCCTGACTGGAAATCGAAGTTCCAGAGCGATTTCGAGAAGCGCTTCGCCATCCCTCACATCACCGACGTCTTCCCCGACGCCGTTCCCATTCCCTCCACCTTCTGCCTCAAGATGAGAACTCCGGTCATCGAAGACTTCGCCGGCGGATATCCGTCCGACGAGGAGTGGCACGGTTACATAAATAACAACGATCGCGTACTTCTCAAGACTATATACTACTCGTCACCTACTTCTGCTGGTGCCGAATGCATTGATCCTAACTGTACTTGGGTGGAGCAATGGGTTCATCGTGCTGGGCCACGAGAAAAGATATATTTTAAACCTGAAGAAGTGAAAGCAGCAATTGTAACATGCGGAGGGCTCTGTCCTGGTCTTAATGATGTCATCAGACAGATTGTCATTACACTTGAAATATATGGTGTAAAAAAGATTGTTGGTATTCCTTACGGTTATCGTGGCTTTTCTGATCCAGAATTAGCCGAAATGCCACTCTCAAGGAAGGTGGTTCAAAATGTTCATCTTTCTGGTGGAAGCTTGTTAGGAGTTTCACGTGGAGGCCCCAGCGTTAGTGAAATAGTGGACAATTTAGAGGAAAGAGGGATCAATATGCTCTTTGTTTTGGGTGGAAACGGTACACATGCTGGAGCCGATGCAATACACACTGAGTGCCGCAAAAGACAGTTAAGGGTGGCTATAGTTGGTGTGCCGAAAACCATAGACAATGACATTTTGTTAATGGACAAAACATTTGGTTTCGATACTGCTGTTGAAGAAGCCCAGCGAGCAATAAATTCAGCATATATTGAGGCACATAGTGCTTACCATGGTATTGGCGTTGTGAAATTGATGGGTCGTAGCAGTGGATTCATAGCAATGCACGCATCCCTGGCTAGTGGACAAATTGACATATGTTTGATACCTGAGGTACCTTTTCAAATACATGGCCCTCATGGTATTCTGCACCATCTGAAATACCTGATTGAAACAAAGGGATCAGCTGTGGTCTGTGTAGCAGAGGGAGCAGGGCAGGTAAGTTATAATTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003872 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008443 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009536 GO:0009987 GO:0016052 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019200 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044262 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046835 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0061615 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko00051 ko00052 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04152 ko05230 map00010 map00030 map00051 map00052 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04152 map05230
Pfam Domains
Protein Families
PFK

Protein Analysis

423

Amino Acids

45.97

Weight (kDa)

7.7

Isoelectric Point (pI)

41.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PFK PF00365 188 - 417 1.5e-56 Phosphofructokinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1263
AatII GACGTC 1 cut(s) 294
Acc36I ACCTGC 1 cut(s) 1243
Acc65I GGTACC 1 cut(s) 1150
AccB1I GGYRCC 2 cut(s) 464, 1150
AccII CGCG 3 cut(s) 26, 118, 420
AciI CCGC 6 cut(s) 39, 55, 58, 369, 579, 895
AclWI GGATC 6 cut(s) 197, 218, 473, 686, 833, 1225
AcsI RAATTY 1 cut(s) 1015
AcuI CTGAAG 1 cut(s) 570
AcvI CACGTG 1 cut(s) 776
AcyI GRCGYC 2 cut(s) 291, 303
AdeI CACNNNGTG 2 cut(s) 891, 1040
AfaI GTAC 4 cut(s) 423, 490, 859, 1152
AfeI AGCGCT 1 cut(s) 266
AgsI TTSAA 5 cut(s) 635, 734, 995, 1160, 1208
AhdI GACNNNNNGTC 1 cut(s) 620
AjnI CCWGG 3 cut(s) 29, 592, 1110
AluBI AGCT 2 cut(s) 759, 1223
AluI AGCT 2 cut(s) 759, 1223
Alw26I GTCTC 1 cut(s) 100
AlwI GGATC 6 cut(s) 197, 218, 473, 686, 833, 1225
Aor51HI AGCGCT 1 cut(s) 266
AoxI GGCC 3 cut(s) 521, 781, 1168
ApeKI GCWGC 3 cut(s) 165, 168, 563
ApoI RAATTY 1 cut(s) 1015
ArsI GACNNNNNNTTYG 2 cut(s) 956, 988
AsiSI GCGATCGC 1 cut(s) 24
Asp700I GAANNNNTTC 1 cut(s) 266
Asp718I GGTACC 1 cut(s) 1150
AspLEI GCGC 3 cut(s) 28, 120, 267
AspS9I GGNCC 3 cut(s) 521, 782, 1169
AsuHPI GGTGA 2 cut(s) 277, 441
AxyI CCTNAGG 1 cut(s) 1146
BaeGI GKGCMC 1 cut(s) 8
BaeI ACNNNNGTAYC 2 cut(s) 975, 1008
BanI GGYRCC 2 cut(s) 464, 1150
BanII GRGCYC 1 cut(s) 588
BauI CACGAG 1 cut(s) 525
BbrPI CACGTG 1 cut(s) 776
BbsI GAAGAC 2 cut(s) 286, 363
BbvI GCAGC 3 cut(s) 177, 180, 575
BccI CCATC 3 cut(s) 281, 1066, 1197
BceAI ACGGC 3 cut(s) 45, 220, 290
BciT130I CCWGG 3 cut(s) 31, 594, 1112
BcoDI GTCTC 1 cut(s) 100
BfaI CTAG 1 cut(s) 1116
BfmI CTRYAG 1 cut(s) 918
BfoI RGCGCY 1 cut(s) 268
BfuAI ACCTGC 1 cut(s) 1243
BisI GCNGC 6 cut(s) 39, 58, 166, 169, 564, 895
BlsI GCNGC 6 cut(s) 40, 59, 167, 170, 565, 896
Bme1390I CCNGG 3 cut(s) 31, 594, 1112
BmeRI GACNNNNNGTC 1 cut(s) 620
BmgT120I GGNCC 3 cut(s) 521, 782, 1169
BmiI GGNNCC 4 cut(s) 466, 784, 871, 1152
BmrFI CCNGG 3 cut(s) 31, 594, 1112
BmsI GCATC 2 cut(s) 865, 1115
BpiI GAAGAC 2 cut(s) 286, 363
BpmI CTGGAG 1 cut(s) 888
BpuEI CTTGAG 3 cut(s) 317, 413, 703
BsaAI YACGTR 1 cut(s) 776
BsaHI GRCGYC 2 cut(s) 291, 303
BsaJI CCNNGG 4 cut(s) 29, 202, 1048, 1110
BsaWI WCCGGW 1 cut(s) 346
Bse118I RCCGGY 1 cut(s) 365
Bse1I ACTGG 1 cut(s) 236
Bse21I CCTNAGG 1 cut(s) 1146
Bse3DI GCAATG 2 cut(s) 509, 1104
BseBI CCWGG 3 cut(s) 31, 594, 1112
BseDI CCNNGG 4 cut(s) 29, 202, 1048, 1110
BseGI GGATG 2 cut(s) 273, 1106
BseMI GCAATG 2 cut(s) 509, 1104
BseMII CTCAG 2 cut(s) 879, 1137
BseNI ACTGG 1 cut(s) 236
BseRI GAGGAG 4 cut(s) 73, 76, 79, 401
BseSI GKGCMC 1 cut(s) 8
BseXI GCAGC 3 cut(s) 177, 180, 575
BseYI CCCAGC 3 cut(s) 518, 785, 1002
BsgI GTGCAG 1 cut(s) 1169
Bsh1236I CGCG 3 cut(s) 26, 118, 420
Bsh1285I CGRYCG 2 cut(s) 24, 418
BshFI GGCC 3 cut(s) 523, 783, 1170
BshNI GGYRCC 2 cut(s) 464, 1150
BsiEI CGRYCG 2 cut(s) 24, 418
BsiSI CCGG 2 cut(s) 347, 366
BsmAI GTCTC 1 cut(s) 100
BsmBI CGTCTC 1 cut(s) 100
BsmI GAATGC 1 cut(s) 476
BsnI GGCC 3 cut(s) 523, 783, 1170
Bsp1286I GDGCHC 2 cut(s) 8, 588
Bsp143I GATC 8 cut(s) 21, 189, 223, 415, 478, 691, 825, 1217
Bsp19I CCATGG 1 cut(s) 1048
BspACI CCGC 6 cut(s) 39, 55, 58, 369, 579, 895
BspANI GGCC 3 cut(s) 523, 783, 1170
BspCNI CTCAG 2 cut(s) 880, 1138
BspFNI CGCG 3 cut(s) 26, 118, 420
BspLI GGNNCC 4 cut(s) 466, 784, 871, 1152
BspMI ACCTGC 1 cut(s) 1243
BspPI GGATC 6 cut(s) 197, 218, 473, 686, 833, 1225
BspQI GCTCTTC 2 cut(s) 127, 155
BspT107I GGYRCC 2 cut(s) 464, 1150
BsrDI GCAATG 2 cut(s) 509, 1104
BsrFI RCCGGY 1 cut(s) 365
BsrI ACTGG 1 cut(s) 236
BssAI RCCGGY 1 cut(s) 365
BssECI CCNNGG 4 cut(s) 29, 202, 1048, 1110
BssMI GATC 8 cut(s) 21, 189, 223, 415, 478, 691, 825, 1217
BssNI GRCGYC 2 cut(s) 291, 303
BssSI CACGAG 1 cut(s) 525
BssT1I CCWWGG 1 cut(s) 1048
Bst2BI CACGAG 1 cut(s) 525
Bst2UI CCWGG 3 cut(s) 31, 594, 1112
Bst4CI ACNGT 6 cut(s) 68, 398, 488, 674, 857, 906
Bst6I CTCTTC 3 cut(s) 103, 127, 155
BstACI GRCGYC 2 cut(s) 291, 303
BstAPI GCANNNNNTGC 1 cut(s) 1040
BstBAI YACGTR 1 cut(s) 776
BstC8I GCNNGC 5 cut(s) 18, 36, 367, 1008, 1104
BstDEI CTNAG 2 cut(s) 888, 1146
BstDSI CCRYGG 2 cut(s) 202, 1048
BstF5I GGATG 2 cut(s) 273, 1106
BstFNI CGCG 3 cut(s) 26, 118, 420
BstH2I RGCGCY 1 cut(s) 268
BstHHI GCGC 3 cut(s) 28, 120, 267
BstKTI GATC 8 cut(s) 24, 192, 226, 418, 481, 694, 828, 1220
BstMAI GTCTC 1 cut(s) 100
BstMBI GATC 8 cut(s) 21, 189, 223, 415, 478, 691, 825, 1217
BstMCI CGRYCG 2 cut(s) 24, 418
BstMWI GCNNNNNNNGC 5 cut(s) 1007, 1040, 1103, 1112, 1244
BstNI CCWGG 3 cut(s) 31, 594, 1112
BstNSI RCATGY 3 cut(s) 20, 579, 866
BstSCI CCNGG 3 cut(s) 29, 592, 1110
BstSFI CTRYAG 1 cut(s) 918
BstSLI GKGCMC 1 cut(s) 8
BstUI CGCG 3 cut(s) 26, 118, 420
BstV1I GCAGC 3 cut(s) 177, 180, 575
BstV2I GAAGAC 2 cut(s) 286, 363
BstXI CCANNNNNNTGG 1 cut(s) 1055
Bsu36I CCTNAGG 1 cut(s) 1146
BsuRI GGCC 3 cut(s) 523, 783, 1170
BtgI CCRYGG 2 cut(s) 202, 1048
BtsCI GGATG 2 cut(s) 273, 1106
BtsI GCAGTG 1 cut(s) 1090
BtsIMutI CAGTG 2 cut(s) 885, 1090
BveI ACCTGC 1 cut(s) 1243
Cac8I GCNNGC 5 cut(s) 18, 36, 367, 1008, 1104
CfoI GCGC 3 cut(s) 28, 120, 267
Cfr10I RCCGGY 1 cut(s) 365
Cfr13I GGNCC 3 cut(s) 521, 782, 1169
CseI GACGC 1 cut(s) 311
Csp6I GTAC 4 cut(s) 422, 489, 858, 1151
CspCI CAANNNNNGTGG 2 cut(s) 708, 743
CviAII CATG 6 cut(s) 17, 576, 863, 1049, 1166, 1175
CviQI GTAC 4 cut(s) 422, 489, 858, 1151
DdeI CTNAG 2 cut(s) 888, 1146
DpnI GATC 8 cut(s) 23, 191, 225, 417, 480, 693, 827, 1219
DpnII GATC 8 cut(s) 21, 189, 223, 415, 478, 691, 825, 1217
DraI TTTAAA 1 cut(s) 544
DraIII CACNNNGTG 2 cut(s) 891, 1040
DriI GACNNNNNGTC 1 cut(s) 620
Eam1104I CTCTTC 3 cut(s) 103, 127, 155
Eam1105I GACNNNNNGTC 1 cut(s) 620
EarI CTCTTC 3 cut(s) 103, 127, 155
EciI GGCGGA 1 cut(s) 384
Eco130I CCWWGG 1 cut(s) 1048
Eco24I GRGCYC 1 cut(s) 588
Eco32I GATATC 1 cut(s) 374
Eco47III AGCGCT 1 cut(s) 266
Eco57I CTGAAG 1 cut(s) 570
Eco72I CACGTG 1 cut(s) 776
Eco81I CCTNAGG 1 cut(s) 1146
EcoO109I RGGNCCY 1 cut(s) 782
EcoRII CCWGG 3 cut(s) 29, 592, 1110
EcoRV GATATC 1 cut(s) 374
EcoT14I CCWWGG 1 cut(s) 1048
EcoT22I ATGCAT 1 cut(s) 476
EcoT38I GRGCYC 1 cut(s) 588
ErhI CCWWGG 1 cut(s) 1048
Esp3I CGTCTC 1 cut(s) 100
FaeI CATG 6 cut(s) 20, 579, 866, 1052, 1169, 1178
FatI CATG 6 cut(s) 16, 575, 862, 1048, 1165, 1174
FauNDI CATATG 1 cut(s) 1133
Fnu4HI GCNGC 6 cut(s) 39, 58, 166, 169, 564, 895
FokI GGATG 2 cut(s) 260, 1093
FriOI GRGCYC 1 cut(s) 588
Fsp4HI GCNGC 6 cut(s) 39, 58, 166, 169, 564, 895
FspBI CTAG 1 cut(s) 1116
GlaI GCGC 3 cut(s) 27, 119, 266
GluI GCNGC 6 cut(s) 39, 58, 166, 169, 564, 895
GsaI CCCAGC 3 cut(s) 522, 789, 1006
GsuI CTGGAG 1 cut(s) 888
HaeII RGCGCY 1 cut(s) 268
HaeIII GGCC 3 cut(s) 523, 783, 1170
HapII CCGG 2 cut(s) 347, 366
HgaI GACGC 1 cut(s) 311
HhaI GCGC 3 cut(s) 28, 120, 267
Hin1I GRCGYC 2 cut(s) 291, 303
Hin1II CATG 6 cut(s) 20, 579, 866, 1052, 1169, 1178
Hin6I GCGC 3 cut(s) 26, 118, 265
HinP1I GCGC 3 cut(s) 26, 118, 265
HindIII AAGCTT 1 cut(s) 757
HinfI GANTC 2 cut(s) 177, 1089
HpaII CCGG 2 cut(s) 347, 366
HphI GGTGA 2 cut(s) 277, 441
Hpy166II GTNNAC 3 cut(s) 805, 860, 1121
Hpy188I TCNGA 4 cut(s) 382, 614, 691, 1194
Hpy188III TCNNGA 8 cut(s) 108, 141, 227, 247, 259, 334, 430, 695
Hpy8I GTNNAC 3 cut(s) 805, 860, 1121
Hpy99I CGWCG 4 cut(s) 154, 293, 305, 386
HpyAV CCTTC 2 cut(s) 334, 718
HpyCH4III ACNGT 6 cut(s) 68, 398, 488, 674, 857, 906
HpyCH4IV ACGT 2 cut(s) 291, 775
HpyCH4V TGCA 4 cut(s) 474, 878, 1102, 1186
HpyF10VI GCNNNNNNNGC 5 cut(s) 1007, 1040, 1103, 1112, 1244
HpyF3I CTNAG 2 cut(s) 888, 1146
HpySE526I ACGT 2 cut(s) 291, 775
Hsp92I GRCGYC 2 cut(s) 291, 303
Hsp92II CATG 6 cut(s) 20, 579, 866, 1052, 1169, 1178
HspAI GCGC 3 cut(s) 26, 118, 265
KpnI GGTACC 1 cut(s) 1154
KroI GCCGGC 1 cut(s) 365
KroNI GCCGGC 1 cut(s) 367
Kzo9I GATC 8 cut(s) 21, 189, 223, 415, 478, 691, 825, 1217
LguI GCTCTTC 2 cut(s) 127, 155
LmnI GCTCC 3 cut(s) 499, 869, 1244
Lsp1109I GCAGC 3 cut(s) 177, 180, 575
LweI GCATC 2 cut(s) 865, 1115
MaeI CTAG 1 cut(s) 1116
MaeII ACGT 2 cut(s) 291, 775
MaeIII GTNAC 4 cut(s) 62, 398, 447, 571
MalI GATC 8 cut(s) 23, 191, 225, 417, 480, 693, 827, 1219
MboI GATC 8 cut(s) 21, 189, 223, 415, 478, 691, 825, 1217
MfeI CAATTG 1 cut(s) 567
MhlI GDGCHC 2 cut(s) 8, 588
MluCI AATT 7 cut(s) 567, 698, 808, 1015, 1067, 1125, 1264
MlyI GAGTC 1 cut(s) 186
MmeI TCCRAC 1 cut(s) 405
Mph1103I ATGCAT 1 cut(s) 476
MreI CGCCGGCG 1 cut(s) 365
MroNI GCCGGC 1 cut(s) 365
MroXI GAANNNNTTC 1 cut(s) 266
MseI TTAA 4 cut(s) 543, 600, 908, 959
MslI CAYNNNNRTG 2 cut(s) 69, 1097
MspA1I CMGCKG 1 cut(s) 1223
MspI CCGG 2 cut(s) 347, 366
MspR9I CCNGG 3 cut(s) 31, 594, 1112
MunI CAATTG 1 cut(s) 567
Mva1269I GAATGC 1 cut(s) 476
MvaI CCWGG 3 cut(s) 31, 594, 1112
MvnI CGCG 3 cut(s) 26, 118, 420
MwoI GCNNNNNNNGC 5 cut(s) 1007, 1040, 1103, 1112, 1244
NaeI GCCGGC 1 cut(s) 367
NcoI CCATGG 1 cut(s) 1048
NdeI CATATG 1 cut(s) 1133
NdeII GATC 8 cut(s) 21, 189, 223, 415, 478, 691, 825, 1217
NgoMIV GCCGGC 1 cut(s) 365
NlaIII CATG 6 cut(s) 20, 579, 866, 1052, 1169, 1178
NlaIV GGNNCC 4 cut(s) 466, 784, 871, 1152
NmuCI GTSAC 2 cut(s) 62, 447
NsiI ATGCAT 1 cut(s) 476
NspI RCATGY 3 cut(s) 20, 579, 866
PaeI GCATGC 1 cut(s) 20
PciSI GCTCTTC 2 cut(s) 127, 155
PcsI WCGNNNNNNNCGW 4 cut(s) 146, 210, 219, 297
PctI GAATGC 1 cut(s) 476
PdiI GCCGGC 1 cut(s) 367
PdmI GAANNNNTTC 1 cut(s) 266
PfeI GAWTC 1 cut(s) 1089
PkrI GCNGC 6 cut(s) 40, 59, 167, 170, 565, 896
Ple19I CGATCG 2 cut(s) 24, 418
PleI GAGTC 1 cut(s) 185
PmaCI CACGTG 1 cut(s) 776
PmlI CACGTG 1 cut(s) 776
PpsI GAGTC 1 cut(s) 185
Ppu21I YACGTR 1 cut(s) 776
PsiI TTATAA 1 cut(s) 1263
Psp6I CCWGG 3 cut(s) 29, 592, 1110
PspCI CACGTG 1 cut(s) 776
PspFI CCCAGC 3 cut(s) 518, 785, 1002
PspGI CCWGG 3 cut(s) 29, 592, 1110
PspN4I GGNNCC 4 cut(s) 466, 784, 871, 1152
PspPI GGNCC 3 cut(s) 521, 782, 1169
PvuI CGATCG 2 cut(s) 24, 418
PvuII CAGCTG 1 cut(s) 1223
RgaI GCGATCGC 1 cut(s) 24
RsaI GTAC 4 cut(s) 423, 490, 859, 1152
RsaNI GTAC 4 cut(s) 422, 489, 858, 1151
RseI CAYNNNNRTG 2 cut(s) 69, 1097
SapI GCTCTTC 2 cut(s) 127, 155
SaqAI TTAA 4 cut(s) 543, 600, 908, 959
SatI GCNGC 6 cut(s) 39, 58, 166, 169, 564, 895
Sau3AI GATC 8 cut(s) 21, 189, 223, 415, 478, 691, 825, 1217
Sau96I GGNCC 3 cut(s) 521, 782, 1169
SchI GAGTC 1 cut(s) 186
ScrFI CCNGG 3 cut(s) 31, 594, 1112
SduI GDGCHC 2 cut(s) 8, 588
SfaAI GCGATCGC 1 cut(s) 24
SfaNI GCATC 2 cut(s) 865, 1115
SfcI CTRYAG 1 cut(s) 918
SgfI GCGATCGC 1 cut(s) 24
SgrAI CRCCGGYG 1 cut(s) 365
SmiMI CAYNNNNRTG 2 cut(s) 69, 1097
SmlI CTYRAG 3 cut(s) 332, 428, 718
SmoI CTYRAG 3 cut(s) 332, 428, 718
SphI GCATGC 1 cut(s) 20
Sse9I AATT 7 cut(s) 567, 698, 808, 1015, 1067, 1125, 1264
SsiI CCGC 6 cut(s) 39, 55, 58, 369, 579, 895
SspMI CTAG 1 cut(s) 1116
StyD4I CCNGG 3 cut(s) 29, 592, 1110
StyI CCWWGG 1 cut(s) 1048
TaaI ACNGT 6 cut(s) 68, 398, 488, 674, 857, 906
TaiI ACGT 2 cut(s) 294, 778
TaqI TCGA 7 cut(s) 109, 175, 213, 239, 258, 354, 981
TasI AATT 7 cut(s) 567, 698, 808, 1015, 1067, 1125, 1264
TatI WGTACW 1 cut(s) 488
TauI GCSGC 3 cut(s) 41, 60, 897
TfiI GAWTC 1 cut(s) 1089
Tru1I TTAA 4 cut(s) 543, 600, 908, 959
Tru9I TTAA 4 cut(s) 543, 600, 908, 959
TscAI CASTG 2 cut(s) 892, 1090
TseFI GTSAC 2 cut(s) 62, 447
TseI GCWGC 3 cut(s) 165, 168, 563
Tsp45I GTSAC 2 cut(s) 62, 447
TspDTI ATGAA 3 cut(s) 500, 731, 1081
TspGWI ACGGA 2 cut(s) 82, 366
TspRI CASTG 2 cut(s) 892, 1090
XapI RAATTY 1 cut(s) 1015
XceI RCATGY 3 cut(s) 20, 579, 866
XmnI GAANNNNTTC 1 cut(s) 266
XspI CTAG 1 cut(s) 1116
ZraI GACGTC 1 cut(s) 292
Zsp2I ATGCAT 1 cut(s) 476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.