FvH4_1g19980

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0008632
Physical Location & Seq
Reverse (-)
12103072 .. 12108918
5847 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g19980.t2

Sequence Viewer

Length: 1614 bp
ATGGGTACTCTCTCGCATGCGATCGCCCCCAGGCTCGCCTCCCCCGCCGTCCACCGCCGCCGTCACGGTAATGGAATATTCTCCTCCTCCGTCTCCTCCTCTTCTCCTTCTTCTCGAAACTCGCGCTCTGCTTCGAGATTGACGAAGAGGAAGAGCGTGAGAGTCGTCGCCGGAATCAAGAACAACCGTGACATCGACTTCAGCGATCCTGATTGGAAATCAAAGTTCCAGAGCGATTTCGAGAAGCGCTTCGCCATCCCTCACATCACCGACGTCTTCCCCGACGCCGTTCCCATTCCCTCCACCTTCTGCCTCAAGATGAGAACTCCGGTGATCGAAGACTTCGCCGGCGGTTATCCCTCCGACGAGGAGTGGCACGGGTACATAAATAACAACGATCGCGTACTTCTCAAGACTATATACTACTCATCACCTACCTCTGCCGGTGCCGAATGCATTGATCCTAATTGTACTTGGGTGGAGCAATGGGTTCATCGTGCTGGGCCACGAGAAAAGATATATTTTAAACCAGAAGAAGTGAAAGCAGCAATTGTCACATGCGGAGGGCTCTGTCCTGGTCTTAATGATGTCATCAGACAGATTGTCATTACACTTGAAATATATGGTGTAAAAAAGATTGTCGGGATTCCTTATGGTTATCGTGGATTTTCTGATAAAGAATTGGCCGAAATGCCACTATCAAGGAAGGTGGTTCAAAATGTTCATCTTTCTGGTGGAAGCTTGTTAGGAGTTTCACGTGGAGGTCCCAGCGTTAGTGAGATTGTGGACAATTTACAGGAAAGAGGGATCAACATGCTCTTTGTTTTGGGTGGAAACGGCACACATGCTGGAGCTGATGCAATACACACTGAGTGCCGCAAAAGACAGTTAAGGGTGGCTATAGTTGGTGTGCCGAAAACCATAGACAATGACATTTTGTTGATGGACAAAACATTTGGTTTTGATACTGCTGTTGAAGAAGCACAGCGAGCAATAAATTCAGCATATATTGAGGCACATAGTGCTTACCATGGTATTGGCATTGTCAAATTGATGGGTCGGAGTAGTGGATTCATAGCAATGCATGCATCCCTGGCTAGTGGACAAATTGACATATGTTTGATACCTGAGGTACCTTTTCAAATACATGGCCCTCATGGTATTCTGCACCATCTGAAATACCTGATTGAAACAAAGGGATCAGCTGTGGTCTGTGTAGCAGAGGGAGCAGGTCAGAATTTAATACAGAAAACTAATGCTAAAGATGCATCGGGAAATGTTGTATTTGGGGATATTGGAGTACATATTCAACAAGAGACAAAGAAACATTTCAAGGAGCTTGGTGTTCCTGTTGATGTGAAATATATAGACCCCACTTACATGATTCGTGCATGTCGTGCAAATGCATCAGATGGTATTCTTTGCGCTGTGCTAGGGCAGAATGCTGTTCATGGTGCATTCGCTGGATATAGTGGCATCACAATTGGCATATGCAACACTCATTATGTATATCTTCCAATTCCTGAGGTTATTTCTCATTCTCGACTGGTGGATCCCAACAGTCGAATGTGGCACCGTTGCCTGACGTCAACAGGCCAGCCAGACTTCATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003872 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008443 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009536 GO:0009987 GO:0016052 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019200 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044262 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046835 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0061615 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko00051 ko00052 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04152 ko05230 map00010 map00030 map00051 map00052 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04152 map05230
Pfam Domains
Protein Families
PFK

Protein Analysis

538

Amino Acids

58.62

Weight (kDa)

8.6

Isoelectric Point (pI)

39.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PFK PF00365 182 - 411 6.6e-57 Phosphofructokinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 276, 1589
Acc36I ACCTGC 1 cut(s) 1220
Acc65I GGTACC 1 cut(s) 1132
AccB1I GGYRCC 3 cut(s) 446, 1132, 1572
AccII CGCG 2 cut(s) 124, 402
AciI CCGC 6 cut(s) 45, 55, 58, 351, 561, 877
AclWI GGATC 6 cut(s) 200, 455, 815, 1207, 1547, 1560
AcoI YGGCCR 1 cut(s) 684
AcsI RAATTY 2 cut(s) 997, 1237
AcuI CTGAAG 1 cut(s) 184
AcvI CACGTG 1 cut(s) 758
AcyI GRCGYC 3 cut(s) 273, 285, 1586
AdeI CACNNNGTG 2 cut(s) 873, 1022
AfaI GTAC 6 cut(s) 7, 383, 405, 472, 1134, 1302
AfeI AGCGCT 1 cut(s) 248
AgsI TTSAA 7 cut(s) 617, 716, 977, 1142, 1190, 1310, 1333
AhdI GACNNNNNGTC 1 cut(s) 602
AjnI CCWGG 3 cut(s) 29, 574, 1092
AluBI AGCT 4 cut(s) 741, 854, 1205, 1339
AluI AGCT 4 cut(s) 741, 854, 1205, 1339
Alw26I GTCTC 2 cut(s) 97, 1310
AlwI GGATC 6 cut(s) 200, 455, 815, 1207, 1547, 1560
Aor51HI AGCGCT 1 cut(s) 248
AoxI GGCC 4 cut(s) 503, 684, 1150, 1594
ApeKI GCWGC 1 cut(s) 545
ApoI RAATTY 2 cut(s) 997, 1237
ArsI GACNNNNNNTTYG 2 cut(s) 938, 970
AsiSI GCGATCGC 1 cut(s) 24
Asp700I GAANNNNTTC 2 cut(s) 248, 1328
Asp718I GGTACC 1 cut(s) 1132
AspLEI GCGC 3 cut(s) 126, 249, 1427
AspS9I GGNCC 3 cut(s) 503, 764, 1151
AsuHPI GGTGA 3 cut(s) 259, 343, 423
AvaII GGWCC 1 cut(s) 764
AxyI CCTNAGG 2 cut(s) 1128, 1524
BaeI ACNNNNGTAYC 2 cut(s) 957, 990
BamHI GGATCC 1 cut(s) 1552
BanI GGYRCC 3 cut(s) 446, 1132, 1572
BanII GRGCYC 1 cut(s) 570
BauI CACGAG 1 cut(s) 507
BbrPI CACGTG 1 cut(s) 758
BbsI GAAGAC 2 cut(s) 268, 345
BbvI GCAGC 1 cut(s) 557
BccI CCATC 5 cut(s) 263, 937, 1048, 1179, 1406
BceAI ACGGC 4 cut(s) 32, 45, 272, 853
BciT130I CCWGG 3 cut(s) 31, 576, 1094
BcoDI GTCTC 2 cut(s) 97, 1310
BfaI CTAG 2 cut(s) 1098, 1433
BfmI CTRYAG 1 cut(s) 900
BfoI RGCGCY 1 cut(s) 250
BfuAI ACCTGC 1 cut(s) 1220
BisI GCNGC 3 cut(s) 58, 546, 877
BlsI GCNGC 3 cut(s) 59, 547, 878
Bme1390I CCNGG 3 cut(s) 31, 576, 1094
Bme18I GGWCC 1 cut(s) 764
BmeRI GACNNNNNGTC 1 cut(s) 602
BmgT120I GGNCC 3 cut(s) 503, 764, 1151
BmiI GGNNCC 5 cut(s) 448, 766, 1134, 1554, 1574
BmrFI CCNGG 3 cut(s) 31, 576, 1094
BmsI GCATC 6 cut(s) 847, 1097, 1255, 1277, 1415, 1485
BpiI GAAGAC 2 cut(s) 268, 345
BpmI CTGGAG 1 cut(s) 870
BpuEI CTTGAG 2 cut(s) 299, 395
BsaAI YACGTR 1 cut(s) 758
BsaHI GRCGYC 3 cut(s) 273, 285, 1586
BsaJI CCNNGG 3 cut(s) 29, 1030, 1092
BsaWI WCCGGW 1 cut(s) 328
Bse118I RCCGGY 2 cut(s) 347, 443
Bse1I ACTGG 1 cut(s) 1551
Bse21I CCTNAGG 2 cut(s) 1128, 1524
Bse3DI GCAATG 2 cut(s) 491, 1086
BseBI CCWGG 3 cut(s) 31, 576, 1094
BseDI CCNNGG 3 cut(s) 29, 1030, 1092
BseGI GGATG 2 cut(s) 255, 1088
BseMI GCAATG 2 cut(s) 491, 1086
BseMII CTCAG 3 cut(s) 861, 1119, 1515
BseNI ACTGG 1 cut(s) 1551
BseRI GAGGAG 5 cut(s) 73, 76, 85, 88, 383
BseXI GCAGC 1 cut(s) 557
BseYI CCCAGC 2 cut(s) 500, 767
BsgI GTGCAG 1 cut(s) 1151
Bsh1236I CGCG 2 cut(s) 124, 402
Bsh1285I CGRYCG 2 cut(s) 24, 400
BshFI GGCC 4 cut(s) 505, 686, 1152, 1596
BshNI GGYRCC 3 cut(s) 446, 1132, 1572
BsiEI CGRYCG 2 cut(s) 24, 400
BsiSI CCGG 4 cut(s) 171, 329, 348, 444
BslFI GGGAC 1 cut(s) 750
BsmAI GTCTC 2 cut(s) 97, 1310
BsmBI CGTCTC 1 cut(s) 97
BsmFI GGGAC 1 cut(s) 750
BsmI GAATGC 3 cut(s) 458, 1447, 1457
BsnI GGCC 4 cut(s) 505, 686, 1152, 1596
Bsp1286I GDGCHC 1 cut(s) 570
Bsp143I GATC 8 cut(s) 21, 205, 333, 397, 460, 807, 1199, 1552
Bsp19I CCATGG 1 cut(s) 1030
BspACI CCGC 6 cut(s) 45, 55, 58, 351, 561, 877
BspANI GGCC 4 cut(s) 505, 686, 1152, 1596
BspCNI CTCAG 3 cut(s) 862, 1120, 1516
BspFNI CGCG 2 cut(s) 124, 402
BspLI GGNNCC 5 cut(s) 448, 766, 1134, 1554, 1574
BspMI ACCTGC 1 cut(s) 1220
BspPI GGATC 6 cut(s) 200, 455, 815, 1207, 1547, 1560
BspQI GCTCTTC 1 cut(s) 146
BspT107I GGYRCC 3 cut(s) 446, 1132, 1572
BsrDI GCAATG 2 cut(s) 491, 1086
BsrFI RCCGGY 2 cut(s) 347, 443
BsrI ACTGG 1 cut(s) 1551
BssAI RCCGGY 2 cut(s) 347, 443
BssECI CCNNGG 3 cut(s) 29, 1030, 1092
BssMI GATC 8 cut(s) 21, 205, 333, 397, 460, 807, 1199, 1552
BssNI GRCGYC 3 cut(s) 273, 285, 1586
BssSI CACGAG 1 cut(s) 507
BssT1I CCWWGG 1 cut(s) 1030
Bst2BI CACGAG 1 cut(s) 507
Bst2UI CCWGG 3 cut(s) 31, 576, 1094
Bst4CI ACNGT 5 cut(s) 68, 188, 888, 1562, 1577
Bst6I CTCTTC 3 cut(s) 106, 140, 146
BstACI GRCGYC 3 cut(s) 273, 285, 1586
BstAPI GCANNNNNTGC 3 cut(s) 1022, 1085, 1397
BstBAI YACGTR 1 cut(s) 758
BstC8I GCNNGC 6 cut(s) 18, 36, 349, 990, 1086, 1598
BstDEI CTNAG 3 cut(s) 870, 1128, 1524
BstDSI CCRYGG 1 cut(s) 1030
BstF5I GGATG 2 cut(s) 255, 1088
BstFNI CGCG 2 cut(s) 124, 402
BstH2I RGCGCY 1 cut(s) 250
BstHHI GCGC 3 cut(s) 126, 249, 1427
BstKTI GATC 8 cut(s) 24, 208, 336, 400, 463, 810, 1202, 1555
BstMAI GTCTC 2 cut(s) 97, 1310
BstMBI GATC 8 cut(s) 21, 205, 333, 397, 460, 807, 1199, 1552
BstMCI CGRYCG 2 cut(s) 24, 400
BstMWI GCNNNNNNNGC 8 cut(s) 44, 989, 1022, 1085, 1094, 1226, 1265, 1397
BstNI CCWGG 3 cut(s) 31, 576, 1094
BstNSI RCATGY 6 cut(s) 20, 561, 817, 848, 1088, 1395
BstSCI CCNGG 3 cut(s) 29, 574, 1092
BstSFI CTRYAG 1 cut(s) 900
BstUI CGCG 2 cut(s) 124, 402
BstV1I GCAGC 1 cut(s) 557
BstV2I GAAGAC 2 cut(s) 268, 345
BstX2I RGATCY 1 cut(s) 1552
BstXI CCANNNNNNTGG 1 cut(s) 1037
BstYI RGATCY 1 cut(s) 1552
Bsu36I CCTNAGG 2 cut(s) 1128, 1524
BsuRI GGCC 4 cut(s) 505, 686, 1152, 1596
BtgI CCRYGG 1 cut(s) 1030
BtsCI GGATG 2 cut(s) 255, 1088
BtsIMutI CAGTG 1 cut(s) 867
BveI ACCTGC 1 cut(s) 1220
Cac8I GCNNGC 6 cut(s) 18, 36, 349, 990, 1086, 1598
CfoI GCGC 3 cut(s) 126, 249, 1427
Cfr10I RCCGGY 2 cut(s) 347, 443
Cfr13I GGNCC 3 cut(s) 503, 764, 1151
CseI GACGC 1 cut(s) 293
Csp6I GTAC 6 cut(s) 6, 382, 404, 471, 1133, 1301
CspCI CAANNNNNGTGG 2 cut(s) 690, 725
CviQI GTAC 6 cut(s) 6, 382, 404, 471, 1133, 1301
DdeI CTNAG 3 cut(s) 870, 1128, 1524
DpnI GATC 8 cut(s) 23, 207, 335, 399, 462, 809, 1201, 1554
DpnII GATC 8 cut(s) 21, 205, 333, 397, 460, 807, 1199, 1552
DraI TTTAAA 1 cut(s) 526
DraIII CACNNNGTG 2 cut(s) 873, 1022
DriI GACNNNNNGTC 1 cut(s) 602
EaeI YGGCCR 1 cut(s) 684
Eam1104I CTCTTC 3 cut(s) 106, 140, 146
Eam1105I GACNNNNNGTC 1 cut(s) 602
EarI CTCTTC 3 cut(s) 106, 140, 146
Eco130I CCWWGG 1 cut(s) 1030
Eco24I GRGCYC 1 cut(s) 570
Eco47I GGWCC 1 cut(s) 764
Eco47III AGCGCT 1 cut(s) 248
Eco57I CTGAAG 1 cut(s) 184
Eco72I CACGTG 1 cut(s) 758
Eco81I CCTNAGG 2 cut(s) 1128, 1524
EcoO109I RGGNCCY 1 cut(s) 764
EcoRII CCWGG 3 cut(s) 29, 574, 1092
EcoT14I CCWWGG 1 cut(s) 1030
EcoT22I ATGCAT 5 cut(s) 458, 1086, 1090, 1270, 1408
EcoT38I GRGCYC 1 cut(s) 570
ErhI CCWWGG 1 cut(s) 1030
Esp3I CGTCTC 1 cut(s) 97
FaqI GGGAC 1 cut(s) 750
FauI CCCGC 1 cut(s) 52
FauNDI CATATG 2 cut(s) 1115, 1490
Fnu4HI GCNGC 3 cut(s) 58, 546, 877
FokI GGATG 2 cut(s) 242, 1075
FriOI GRGCYC 1 cut(s) 570
Fsp4HI GCNGC 3 cut(s) 58, 546, 877
FspBI CTAG 2 cut(s) 1098, 1433
GlaI GCGC 3 cut(s) 125, 248, 1426
GluI GCNGC 3 cut(s) 58, 546, 877
GsaI CCCAGC 2 cut(s) 504, 771
GsuI CTGGAG 1 cut(s) 870
HaeII RGCGCY 1 cut(s) 250
HaeIII GGCC 4 cut(s) 505, 686, 1152, 1596
HapII CCGG 4 cut(s) 171, 329, 348, 444
HgaI GACGC 1 cut(s) 293
HhaI GCGC 3 cut(s) 126, 249, 1427
Hin1I GRCGYC 3 cut(s) 273, 285, 1586
Hin6I GCGC 3 cut(s) 124, 247, 1425
HinP1I GCGC 3 cut(s) 124, 247, 1425
HincII GTYRAC 1 cut(s) 1590
HindII GTYRAC 1 cut(s) 1590
HindIII AAGCTT 1 cut(s) 739
HinfI GANTC 5 cut(s) 162, 174, 646, 1071, 1384
HpaII CCGG 4 cut(s) 171, 329, 348, 444
HphI GGTGA 3 cut(s) 259, 343, 423
Hpy166II GTNNAC 4 cut(s) 52, 787, 1103, 1590
Hpy188I TCNGA 7 cut(s) 364, 596, 673, 1062, 1176, 1236, 1411
Hpy8I GTNNAC 4 cut(s) 52, 787, 1103, 1590
Hpy99I CGWCG 4 cut(s) 170, 275, 287, 368
HpyAV CCTTC 3 cut(s) 117, 316, 700
HpyCH4III ACNGT 5 cut(s) 68, 188, 888, 1562, 1577
HpyCH4IV ACGT 3 cut(s) 273, 757, 1586
HpyF10VI GCNNNNNNNGC 8 cut(s) 44, 989, 1022, 1085, 1094, 1226, 1265, 1397
HpyF3I CTNAG 3 cut(s) 870, 1128, 1524
HpySE526I ACGT 3 cut(s) 273, 757, 1586
Hsp92I GRCGYC 3 cut(s) 273, 285, 1586
HspAI GCGC 3 cut(s) 124, 247, 1425
KpnI GGTACC 1 cut(s) 1136
KroI GCCGGC 1 cut(s) 347
KroNI GCCGGC 1 cut(s) 349
Kzo9I GATC 8 cut(s) 21, 205, 333, 397, 460, 807, 1199, 1552
LguI GCTCTTC 1 cut(s) 146
LmnI GCTCC 4 cut(s) 481, 851, 1226, 1336
Lsp1109I GCAGC 1 cut(s) 557
LweI GCATC 6 cut(s) 847, 1097, 1255, 1277, 1415, 1485
MaeI CTAG 2 cut(s) 1098, 1433
MaeII ACGT 3 cut(s) 273, 757, 1586
MaeIII GTNAC 3 cut(s) 62, 188, 553
MalI GATC 8 cut(s) 23, 207, 335, 399, 462, 809, 1201, 1554
MboI GATC 8 cut(s) 21, 205, 333, 397, 460, 807, 1199, 1552
MboII GAAGA 9 cut(s) 93, 102, 157, 163, 268, 350, 545, 989, 1505
MfeI CAATTG 2 cut(s) 549, 1482
MflI RGATCY 1 cut(s) 1552
MhlI GDGCHC 1 cut(s) 570
MlyI GAGTC 1 cut(s) 171
MmeI TCCRAC 2 cut(s) 387, 1040
Mph1103I ATGCAT 5 cut(s) 458, 1086, 1090, 1270, 1408
MreI CGCCGGCG 1 cut(s) 347
MroNI GCCGGC 1 cut(s) 347
MroXI GAANNNNTTC 2 cut(s) 248, 1328
MseI TTAA 4 cut(s) 525, 582, 890, 1241
MslI CAYNNNNRTG 3 cut(s) 69, 1079, 1379
MspA1I CMGCKG 1 cut(s) 1205
MspI CCGG 4 cut(s) 171, 329, 348, 444
MspR9I CCNGG 3 cut(s) 31, 576, 1094
MunI CAATTG 2 cut(s) 549, 1482
Mva1269I GAATGC 3 cut(s) 458, 1447, 1457
MvaI CCWGG 3 cut(s) 31, 576, 1094
MvnI CGCG 2 cut(s) 124, 402
MwoI GCNNNNNNNGC 8 cut(s) 44, 989, 1022, 1085, 1094, 1226, 1265, 1397
NaeI GCCGGC 1 cut(s) 349
NcoI CCATGG 1 cut(s) 1030
NdeI CATATG 2 cut(s) 1115, 1490
NdeII GATC 8 cut(s) 21, 205, 333, 397, 460, 807, 1199, 1552
NgoMIV GCCGGC 1 cut(s) 347
NlaIV GGNNCC 5 cut(s) 448, 766, 1134, 1554, 1574
NmuCI GTSAC 3 cut(s) 62, 188, 553
NsiI ATGCAT 5 cut(s) 458, 1086, 1090, 1270, 1408
NspI RCATGY 6 cut(s) 20, 561, 817, 848, 1088, 1395
PaeI GCATGC 2 cut(s) 20, 1088
PciSI GCTCTTC 1 cut(s) 146
PcsI WCGNNNNNNNCGW 4 cut(s) 140, 201, 279, 1393
PctI GAATGC 3 cut(s) 458, 1447, 1457
PdiI GCCGGC 1 cut(s) 349
PdmI GAANNNNTTC 2 cut(s) 248, 1328
PfeI GAWTC 4 cut(s) 174, 646, 1071, 1384
PkrI GCNGC 3 cut(s) 59, 547, 878
Ple19I CGATCG 2 cut(s) 24, 400
PleI GAGTC 1 cut(s) 170
PmaCI CACGTG 1 cut(s) 758
PmlI CACGTG 1 cut(s) 758
PpsI GAGTC 1 cut(s) 170
Ppu21I YACGTR 1 cut(s) 758
PpuMI RGGWCCY 1 cut(s) 764
Psp5II RGGWCCY 1 cut(s) 764
Psp6I CCWGG 3 cut(s) 29, 574, 1092
PspCI CACGTG 1 cut(s) 758
PspFI CCCAGC 2 cut(s) 500, 767
PspGI CCWGG 3 cut(s) 29, 574, 1092
PspN4I GGNNCC 5 cut(s) 448, 766, 1134, 1554, 1574
PspPI GGNCC 3 cut(s) 503, 764, 1151
PspPPI RGGWCCY 1 cut(s) 764
PsuI RGATCY 1 cut(s) 1552
PvuI CGATCG 2 cut(s) 24, 400
PvuII CAGCTG 1 cut(s) 1205
RgaI GCGATCGC 1 cut(s) 24
RsaI GTAC 6 cut(s) 7, 383, 405, 472, 1134, 1302
RsaNI GTAC 6 cut(s) 6, 382, 404, 471, 1133, 1301
RseI CAYNNNNRTG 3 cut(s) 69, 1079, 1379
SapI GCTCTTC 1 cut(s) 146
SaqAI TTAA 4 cut(s) 525, 582, 890, 1241
SatI GCNGC 3 cut(s) 58, 546, 877
Sau3AI GATC 8 cut(s) 21, 205, 333, 397, 460, 807, 1199, 1552
Sau96I GGNCC 3 cut(s) 503, 764, 1151
SchI GAGTC 1 cut(s) 171
ScrFI CCNGG 3 cut(s) 31, 576, 1094
SduI GDGCHC 1 cut(s) 570
SfaAI GCGATCGC 1 cut(s) 24
SfaNI GCATC 6 cut(s) 847, 1097, 1255, 1277, 1415, 1485
SfcI CTRYAG 1 cut(s) 900
SgfI GCGATCGC 1 cut(s) 24
SgrAI CRCCGGYG 1 cut(s) 347
SinI GGWCC 1 cut(s) 764
SmiMI CAYNNNNRTG 3 cut(s) 69, 1079, 1379
SmlI CTYRAG 2 cut(s) 314, 410
SmoI CTYRAG 2 cut(s) 314, 410
SphI GCATGC 2 cut(s) 20, 1088
SsiI CCGC 6 cut(s) 45, 55, 58, 351, 561, 877
SspI AATATT 1 cut(s) 78
SspMI CTAG 2 cut(s) 1098, 1433
StyD4I CCNGG 3 cut(s) 29, 574, 1092
StyI CCWWGG 1 cut(s) 1030
TaaI ACNGT 5 cut(s) 68, 188, 888, 1562, 1577
TaiI ACGT 3 cut(s) 276, 760, 1589
TaqI TCGA 7 cut(s) 115, 134, 195, 240, 336, 1543, 1564
TatI WGTACW 2 cut(s) 470, 1300
TauI GCSGC 2 cut(s) 60, 879
TfiI GAWTC 4 cut(s) 174, 646, 1071, 1384
Tru1I TTAA 4 cut(s) 525, 582, 890, 1241
Tru9I TTAA 4 cut(s) 525, 582, 890, 1241
TscAI CASTG 1 cut(s) 874
TseFI GTSAC 3 cut(s) 62, 188, 553
TseI GCWGC 1 cut(s) 545
Tsp45I GTSAC 3 cut(s) 62, 188, 553
TspDTI ATGAA 5 cut(s) 482, 713, 1063, 1439, 1597
TspGWI ACGGA 1 cut(s) 79
TspRI CASTG 1 cut(s) 874
VpaK11BI GGWCC 1 cut(s) 764
XapI RAATTY 2 cut(s) 997, 1237
XceI RCATGY 6 cut(s) 20, 561, 817, 848, 1088, 1395
XmnI GAANNNNTTC 2 cut(s) 248, 1328
XspI CTAG 2 cut(s) 1098, 1433
ZraI GACGTC 2 cut(s) 274, 1587
Zsp2I ATGCAT 5 cut(s) 458, 1086, 1090, 1270, 1408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.