Rw2G017760

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0008632
Physical Location & Seq
Reverse (-)
22096480 .. 22101602
5123 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G017760.1

Sequence Viewer

Length: 1614 bp
ATGGGCACTCTCTCGCATGCGATCGCGCCCAGGCTCGCCGCCCTCCACCACCACCGCCGCCGTCACGGTAATGGAGTATTCTCCTCCTCCTCCGTCTCTTCTTCTTCTTCTTCTTCTGCTTCGGGATTGACGACGAAGAAGAGGAGCAGCAGCGTTCGAGTCTTGGCAGGGATCAAGCACAACCACAGCGACCTCGATTTCAGCGATCCTGACTGGAAATCAAAGTTCCAGAGCGATTTCGAGAAGCGCTTCGCCATCCCTCACATCACCGACGTCTTCCCCGGCGCCGTTCCCATTCCCTCCACCTTCTGCCTCAAGATGAGAACTCCGGTCATCGAAGACTTCGCCGGCGGATATCCCTCCGACGAGGAGTGGCACGGTTACATAAATAACAACGATCGCGTACTTCTCAAGACTATATACTACTCGTCACCTACGTCTGCTGGTGCCGAATGCATTGATCCTAACTGTACTTGGGTGGAGCAATGGGTTCATCGTGCTGGGCCACGAGAAAAGATATATTTTAAACCTGAAGAAGTGAAAGCAGCAATTGTAACATGCGGAGGGCTCTGTCCTGGTCTTAATGATGTCATCAGACAGATTGTCATTACACTTGAAATATATGGTGTAAAAAAGATTGTTGGTATTCCTTACGGTTATCGTGGCTTTTCTGATCCAGAATTAGCCGAAATGCCACTCTCAAGGAAGGTGGTTCAAAATGTTCATCTTTCTGGTGGAAGCTTGTTAGGAGTTTCACGTGGAGGCCCCAGCGTTAGTGAAATAGTGGACAATTTAGAGGAAAGAGGGATCAATATGCTCTTTGTTTTGGGTGGAAACGGCACACATGCTGGAGCCGATGCAATACACACTGAGTGCCGCAAAAGACAGTTAAGGGTGGCTATAGTTGGTGTGCCGAAAACCATAGACAATGACATTTTGTTAATGGACAAAACATTTGGTTTCGATACCGCTGTTGAAGAAGCCCAGCGAGCAATAAATTCAGCATATATTGAGGCACATAGTGCTTACCATGGTATTGGCGTTGTGAAATTGATGGGTCGTAGCAGTGGATTCATAGCAATGCACGCATCCCTGGCTAGTGGACAAATTGACATATGTTTGATACCTGAGGTACCTTTTCAAATACATGGCCCTCATGGTATTCTGCACCATCTGAAATACCTGATTGAAACAAAGGGATCAGCTGTGGTCTGTGTAGCAGAGGGAGCAGGGCAGAATTTACTACAGAAAACCAATGCTACTGATGCATCAGGAAATGTTGTATTTGGGGATATTGGAGTACATATTCAACAAGAGACAAAGAAACATTTTAAGGAGCATGGTGTTCCTGTTGATGTGAAATATATAGACCCCACATACATGATTCGTGCATGTCGTGCAAATGCATCAGATGGAATTCTTTGCGCTGTGCTAGGGCAGAATGCCGTTCATGGTGCATTTGCTGGATATAGTGGCATTACAATTGGCATATGCAACACTCATTATGTATATCTTCCGATTCCTGAGGTTATTTCTCATCCCCGACTGGTGGATCCCAACAGTCGAATGTGGCACCGTTGCTTGACTTCAACAGGCCAGCCAGACTTCATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003872 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008443 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009536 GO:0009987 GO:0016052 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019200 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044262 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046835 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0061615 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko00051 ko00052 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04152 ko05230 map00010 map00030 map00051 map00052 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04152 map05230
Pfam Domains
Protein Families
PFK

Protein Analysis

537

Amino Acids

58.37

Weight (kDa)

7.38

Isoelectric Point (pI)

38.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PFK PF00365 182 - 484 6.8e-62 Phosphofructokinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 276
Acc65I GGTACC 1 cut(s) 1132
AccB1I GGYRCC 4 cut(s) 284, 446, 1132, 1572
AccII CGCG 2 cut(s) 26, 402
AciI CCGC 7 cut(s) 39, 55, 58, 351, 561, 877, 969
AclWI GGATC 8 cut(s) 179, 200, 455, 668, 815, 1207, 1547, 1560
AcsI RAATTY 3 cut(s) 997, 1237, 1416
AcuI CTGAAG 1 cut(s) 552
AcvI CACGTG 1 cut(s) 758
AcyI GRCGYC 2 cut(s) 273, 285
AdeI CACNNNGTG 2 cut(s) 873, 1022
AfaI GTAC 4 cut(s) 405, 472, 1134, 1302
AfeI AGCGCT 1 cut(s) 248
AfiI CCNNNNNNNGG 1 cut(s) 1549
AgsI TTSAA 7 cut(s) 617, 716, 977, 1142, 1190, 1310, 1590
AhdI GACNNNNNGTC 1 cut(s) 602
AjnI CCWGG 3 cut(s) 29, 574, 1092
AluBI AGCT 2 cut(s) 741, 1205
AluI AGCT 2 cut(s) 741, 1205
Alw26I GTCTC 2 cut(s) 100, 1310
AlwI GGATC 8 cut(s) 179, 200, 455, 668, 815, 1207, 1547, 1560
Aor51HI AGCGCT 1 cut(s) 248
AoxI GGCC 4 cut(s) 503, 763, 1150, 1594
ApeKI GCWGC 3 cut(s) 147, 150, 545
ApoI RAATTY 3 cut(s) 997, 1237, 1416
ArsI GACNNNNNNTTYG 2 cut(s) 938, 970
AsiSI GCGATCGC 1 cut(s) 24
Asp700I GAANNNNTTC 1 cut(s) 248
Asp718I GGTACC 1 cut(s) 1132
AspLEI GCGC 4 cut(s) 28, 249, 287, 1427
AspS9I GGNCC 3 cut(s) 503, 764, 1151
AsuC2I CCSGG 1 cut(s) 282
AsuHPI GGTGA 2 cut(s) 259, 423
AxyI CCTNAGG 2 cut(s) 1128, 1524
BaeGI GKGCMC 1 cut(s) 8
BaeI ACNNNNGTAYC 2 cut(s) 957, 990
BamHI GGATCC 1 cut(s) 1552
BanI GGYRCC 4 cut(s) 284, 446, 1132, 1572
BanII GRGCYC 1 cut(s) 570
BauI CACGAG 1 cut(s) 507
BbrPI CACGTG 1 cut(s) 758
BbsI GAAGAC 2 cut(s) 268, 345
BbvI GCAGC 3 cut(s) 159, 162, 557
BccI CCATC 4 cut(s) 263, 1048, 1179, 1406
BceAI ACGGC 4 cut(s) 45, 272, 853, 1430
BciT130I CCWGG 3 cut(s) 31, 576, 1094
BcnI CCSGG 1 cut(s) 282
BcoDI GTCTC 2 cut(s) 100, 1310
BfaI CTAG 2 cut(s) 1098, 1433
BfmI CTRYAG 2 cut(s) 900, 1244
BfoI RGCGCY 2 cut(s) 250, 288
BisI GCNGC 6 cut(s) 39, 58, 148, 151, 546, 877
BlsI GCNGC 6 cut(s) 40, 59, 149, 152, 547, 878
Bme1390I CCNGG 4 cut(s) 31, 282, 576, 1094
BmeRI GACNNNNNGTC 1 cut(s) 602
BmgT120I GGNCC 3 cut(s) 503, 764, 1151
BmiI GGNNCC 7 cut(s) 286, 448, 766, 853, 1134, 1554, 1574
BmrFI CCNGG 4 cut(s) 31, 282, 576, 1094
BmsI GCATC 5 cut(s) 847, 1097, 1255, 1277, 1415
BpiI GAAGAC 2 cut(s) 268, 345
BpmI CTGGAG 1 cut(s) 870
BpuEI CTTGAG 3 cut(s) 299, 395, 685
BpuMI CCSGG 1 cut(s) 282
BsaAI YACGTR 1 cut(s) 758
BsaHI GRCGYC 2 cut(s) 273, 285
BsaJI CCNNGG 4 cut(s) 29, 280, 1030, 1092
BsaWI WCCGGW 1 cut(s) 328
Bsc4I CCNNNNNNNGG 1 cut(s) 1549
Bse118I RCCGGY 1 cut(s) 347
Bse1I ACTGG 2 cut(s) 218, 1551
Bse21I CCTNAGG 2 cut(s) 1128, 1524
Bse3DI GCAATG 2 cut(s) 491, 1086
BseBI CCWGG 3 cut(s) 31, 576, 1094
BseDI CCNNGG 4 cut(s) 29, 280, 1030, 1092
BseGI GGATG 3 cut(s) 255, 1088, 1537
BseLI CCNNNNNNNGG 1 cut(s) 1549
BseMI GCAATG 2 cut(s) 491, 1086
BseMII CTCAG 3 cut(s) 861, 1119, 1515
BseNI ACTGG 2 cut(s) 218, 1551
BseRI GAGGAG 5 cut(s) 73, 76, 79, 157, 383
BseSI GKGCMC 1 cut(s) 8
BseXI GCAGC 3 cut(s) 159, 162, 557
BseYI CCCAGC 3 cut(s) 500, 767, 984
BsgI GTGCAG 1 cut(s) 1151
Bsh1236I CGCG 2 cut(s) 26, 402
Bsh1285I CGRYCG 2 cut(s) 24, 400
BshFI GGCC 4 cut(s) 505, 765, 1152, 1596
BshNI GGYRCC 4 cut(s) 284, 446, 1132, 1572
BsiEI CGRYCG 2 cut(s) 24, 400
BsiSI CCGG 3 cut(s) 282, 329, 348
BslI CCNNNNNNNGG 1 cut(s) 1549
BsmAI GTCTC 2 cut(s) 100, 1310
BsmBI CGTCTC 1 cut(s) 100
BsmI GAATGC 2 cut(s) 458, 1447
BsnI GGCC 4 cut(s) 505, 765, 1152, 1596
Bsp1286I GDGCHC 2 cut(s) 8, 570
Bsp143I GATC 9 cut(s) 21, 171, 205, 397, 460, 673, 807, 1199, 1552
Bsp19I CCATGG 1 cut(s) 1030
BspACI CCGC 7 cut(s) 39, 55, 58, 351, 561, 877, 969
BspANI GGCC 4 cut(s) 505, 765, 1152, 1596
BspCNI CTCAG 3 cut(s) 862, 1120, 1516
BspFNI CGCG 2 cut(s) 26, 402
BspLI GGNNCC 7 cut(s) 286, 448, 766, 853, 1134, 1554, 1574
BspPI GGATC 8 cut(s) 179, 200, 455, 668, 815, 1207, 1547, 1560
BspT107I GGYRCC 4 cut(s) 284, 446, 1132, 1572
BsrDI GCAATG 2 cut(s) 491, 1086
BsrFI RCCGGY 1 cut(s) 347
BsrI ACTGG 2 cut(s) 218, 1551
BssAI RCCGGY 1 cut(s) 347
BssECI CCNNGG 4 cut(s) 29, 280, 1030, 1092
BssMI GATC 9 cut(s) 21, 171, 205, 397, 460, 673, 807, 1199, 1552
BssNI GRCGYC 2 cut(s) 273, 285
BssSI CACGAG 1 cut(s) 507
BssT1I CCWWGG 1 cut(s) 1030
Bst2BI CACGAG 1 cut(s) 507
Bst2UI CCWGG 3 cut(s) 31, 576, 1094
Bst4CI ACNGT 7 cut(s) 68, 380, 470, 656, 888, 1562, 1577
Bst6I CTCTTC 2 cut(s) 103, 134
BstACI GRCGYC 2 cut(s) 273, 285
BstAPI GCANNNNNTGC 2 cut(s) 1022, 1397
BstBAI YACGTR 1 cut(s) 758
BstC8I GCNNGC 6 cut(s) 18, 36, 349, 990, 1086, 1598
BstDEI CTNAG 3 cut(s) 870, 1128, 1524
BstDSI CCRYGG 1 cut(s) 1030
BstF5I GGATG 3 cut(s) 255, 1088, 1537
BstFNI CGCG 2 cut(s) 26, 402
BstH2I RGCGCY 2 cut(s) 250, 288
BstHHI GCGC 4 cut(s) 28, 249, 287, 1427
BstKTI GATC 9 cut(s) 24, 174, 208, 400, 463, 676, 810, 1202, 1555
BstMAI GTCTC 2 cut(s) 100, 1310
BstMBI GATC 9 cut(s) 21, 171, 205, 397, 460, 673, 807, 1199, 1552
BstMCI CGRYCG 2 cut(s) 24, 400
BstMWI GCNNNNNNNGC 7 cut(s) 989, 1022, 1085, 1094, 1226, 1265, 1397
BstNI CCWGG 3 cut(s) 31, 576, 1094
BstNSI RCATGY 4 cut(s) 20, 561, 848, 1395
BstSCI CCNGG 4 cut(s) 29, 280, 574, 1092
BstSFI CTRYAG 2 cut(s) 900, 1244
BstSLI GKGCMC 1 cut(s) 8
BstUI CGCG 2 cut(s) 26, 402
BstV1I GCAGC 3 cut(s) 159, 162, 557
BstV2I GAAGAC 2 cut(s) 268, 345
BstX2I RGATCY 1 cut(s) 1552
BstXI CCANNNNNNTGG 1 cut(s) 1037
BstYI RGATCY 1 cut(s) 1552
Bsu36I CCTNAGG 2 cut(s) 1128, 1524
BsuRI GGCC 4 cut(s) 505, 765, 1152, 1596
BtgI CCRYGG 1 cut(s) 1030
BtsCI GGATG 3 cut(s) 255, 1088, 1537
BtsI GCAGTG 1 cut(s) 1072
BtsIMutI CAGTG 2 cut(s) 867, 1072
Cac8I GCNNGC 6 cut(s) 18, 36, 349, 990, 1086, 1598
CfoI GCGC 4 cut(s) 28, 249, 287, 1427
Cfr10I RCCGGY 1 cut(s) 347
Cfr13I GGNCC 3 cut(s) 503, 764, 1151
Csp6I GTAC 4 cut(s) 404, 471, 1133, 1301
CspCI CAANNNNNGTGG 2 cut(s) 690, 725
CviQI GTAC 4 cut(s) 404, 471, 1133, 1301
DdeI CTNAG 3 cut(s) 870, 1128, 1524
DinI GGCGCC 1 cut(s) 286
DpnI GATC 9 cut(s) 23, 173, 207, 399, 462, 675, 809, 1201, 1554
DpnII GATC 9 cut(s) 21, 171, 205, 397, 460, 673, 807, 1199, 1552
DraI TTTAAA 1 cut(s) 526
DraIII CACNNNGTG 2 cut(s) 873, 1022
DriI GACNNNNNGTC 1 cut(s) 602
Eam1104I CTCTTC 2 cut(s) 103, 134
Eam1105I GACNNNNNGTC 1 cut(s) 602
EarI CTCTTC 2 cut(s) 103, 134
EciI GGCGGA 1 cut(s) 366
Eco130I CCWWGG 1 cut(s) 1030
Eco24I GRGCYC 1 cut(s) 570
Eco32I GATATC 1 cut(s) 356
Eco47III AGCGCT 1 cut(s) 248
Eco57I CTGAAG 1 cut(s) 552
Eco72I CACGTG 1 cut(s) 758
Eco81I CCTNAGG 2 cut(s) 1128, 1524
EcoO109I RGGNCCY 1 cut(s) 764
EcoRI GAATTC 1 cut(s) 1416
EcoRII CCWGG 3 cut(s) 29, 574, 1092
EcoRV GATATC 1 cut(s) 356
EcoT14I CCWWGG 1 cut(s) 1030
EcoT22I ATGCAT 3 cut(s) 458, 1270, 1408
EcoT38I GRGCYC 1 cut(s) 570
EgeI GGCGCC 1 cut(s) 286
EheI GGCGCC 1 cut(s) 286
ErhI CCWWGG 1 cut(s) 1030
Esp3I CGTCTC 1 cut(s) 100
FauNDI CATATG 2 cut(s) 1115, 1490
Fnu4HI GCNGC 6 cut(s) 39, 58, 148, 151, 546, 877
FokI GGATG 3 cut(s) 242, 1075, 1524
FriOI GRGCYC 1 cut(s) 570
Fsp4HI GCNGC 6 cut(s) 39, 58, 148, 151, 546, 877
FspBI CTAG 2 cut(s) 1098, 1433
GlaI GCGC 4 cut(s) 27, 248, 286, 1426
GluI GCNGC 6 cut(s) 39, 58, 148, 151, 546, 877
GsaI CCCAGC 3 cut(s) 504, 771, 988
GsuI CTGGAG 1 cut(s) 870
HaeII RGCGCY 2 cut(s) 250, 288
HaeIII GGCC 4 cut(s) 505, 765, 1152, 1596
HapII CCGG 3 cut(s) 282, 329, 348
HhaI GCGC 4 cut(s) 28, 249, 287, 1427
Hin1I GRCGYC 2 cut(s) 273, 285
Hin6I GCGC 4 cut(s) 26, 247, 285, 1425
HinP1I GCGC 4 cut(s) 26, 247, 285, 1425
HindIII AAGCTT 1 cut(s) 739
HinfI GANTC 4 cut(s) 159, 1071, 1384, 1519
HpaII CCGG 3 cut(s) 282, 329, 348
HphI GGTGA 2 cut(s) 259, 423
Hpy166II GTNNAC 2 cut(s) 787, 1103
Hpy188I TCNGA 6 cut(s) 364, 596, 673, 1176, 1411, 1518
Hpy188III TCNNGA 9 cut(s) 123, 209, 229, 241, 316, 412, 677, 1272, 1523
Hpy8I GTNNAC 2 cut(s) 787, 1103
Hpy99I CGWCG 3 cut(s) 136, 275, 368
HpyAV CCTTC 2 cut(s) 316, 700
HpyCH4III ACNGT 7 cut(s) 68, 380, 470, 656, 888, 1562, 1577
HpyCH4IV ACGT 3 cut(s) 273, 437, 757
HpyF10VI GCNNNNNNNGC 7 cut(s) 989, 1022, 1085, 1094, 1226, 1265, 1397
HpyF3I CTNAG 3 cut(s) 870, 1128, 1524
HpySE526I ACGT 3 cut(s) 273, 437, 757
Hsp92I GRCGYC 2 cut(s) 273, 285
HspAI GCGC 4 cut(s) 26, 247, 285, 1425
KasI GGCGCC 1 cut(s) 284
KpnI GGTACC 1 cut(s) 1136
KroI GCCGGC 1 cut(s) 347
KroNI GCCGGC 1 cut(s) 349
Kzo9I GATC 9 cut(s) 21, 171, 205, 397, 460, 673, 807, 1199, 1552
LmnI GCTCC 5 cut(s) 144, 481, 851, 1226, 1336
Lsp1109I GCAGC 3 cut(s) 159, 162, 557
LweI GCATC 5 cut(s) 847, 1097, 1255, 1277, 1415
MaeI CTAG 2 cut(s) 1098, 1433
MaeII ACGT 3 cut(s) 273, 437, 757
MaeIII GTNAC 4 cut(s) 62, 380, 429, 553
MalI GATC 9 cut(s) 23, 173, 207, 399, 462, 675, 809, 1201, 1554
MboI GATC 9 cut(s) 21, 171, 205, 397, 460, 673, 807, 1199, 1552
MfeI CAATTG 2 cut(s) 549, 1482
MflI RGATCY 1 cut(s) 1552
MhlI GDGCHC 2 cut(s) 8, 570
MluCI AATT 9 cut(s) 549, 680, 790, 997, 1049, 1107, 1237, 1416, 1482
Mly113I GGCGCC 1 cut(s) 285
MlyI GAGTC 1 cut(s) 168
MmeI TCCRAC 1 cut(s) 387
Mph1103I ATGCAT 3 cut(s) 458, 1270, 1408
MreI CGCCGGCG 1 cut(s) 347
MroNI GCCGGC 1 cut(s) 347
MroXI GAANNNNTTC 1 cut(s) 248
MseI TTAA 5 cut(s) 525, 582, 890, 941, 1332
MslI CAYNNNNRTG 3 cut(s) 69, 1079, 1379
MspA1I CMGCKG 2 cut(s) 971, 1205
MspI CCGG 3 cut(s) 282, 329, 348
MspR9I CCNGG 4 cut(s) 31, 282, 576, 1094
MunI CAATTG 2 cut(s) 549, 1482
Mva1269I GAATGC 2 cut(s) 458, 1447
MvaI CCWGG 3 cut(s) 31, 576, 1094
MvnI CGCG 2 cut(s) 26, 402
MwoI GCNNNNNNNGC 7 cut(s) 989, 1022, 1085, 1094, 1226, 1265, 1397
NaeI GCCGGC 1 cut(s) 349
NarI GGCGCC 1 cut(s) 285
NciI CCSGG 1 cut(s) 282
NcoI CCATGG 1 cut(s) 1030
NdeI CATATG 2 cut(s) 1115, 1490
NdeII GATC 9 cut(s) 21, 171, 205, 397, 460, 673, 807, 1199, 1552
NgoMIV GCCGGC 1 cut(s) 347
NlaIV GGNNCC 7 cut(s) 286, 448, 766, 853, 1134, 1554, 1574
NmuCI GTSAC 2 cut(s) 62, 429
NsiI ATGCAT 3 cut(s) 458, 1270, 1408
NspI RCATGY 4 cut(s) 20, 561, 848, 1395
PaeI GCATGC 1 cut(s) 20
PcsI WCGNNNNNNNCGW 4 cut(s) 128, 201, 434, 1393
PctI GAATGC 2 cut(s) 458, 1447
PdiI GCCGGC 1 cut(s) 349
PdmI GAANNNNTTC 1 cut(s) 248
PfeI GAWTC 3 cut(s) 1071, 1384, 1519
PkrI GCNGC 6 cut(s) 40, 59, 149, 152, 547, 878
Ple19I CGATCG 2 cut(s) 24, 400
PleI GAGTC 1 cut(s) 167
PluTI GGCGCC 1 cut(s) 288
PmaCI CACGTG 1 cut(s) 758
PmlI CACGTG 1 cut(s) 758
PpsI GAGTC 1 cut(s) 167
Ppu21I YACGTR 1 cut(s) 758
Psp6I CCWGG 3 cut(s) 29, 574, 1092
PspCI CACGTG 1 cut(s) 758
PspFI CCCAGC 3 cut(s) 500, 767, 984
PspGI CCWGG 3 cut(s) 29, 574, 1092
PspN4I GGNNCC 7 cut(s) 286, 448, 766, 853, 1134, 1554, 1574
PspPI GGNCC 3 cut(s) 503, 764, 1151
PsuI RGATCY 1 cut(s) 1552
PvuI CGATCG 2 cut(s) 24, 400
PvuII CAGCTG 1 cut(s) 1205
RgaI GCGATCGC 1 cut(s) 24
RsaI GTAC 4 cut(s) 405, 472, 1134, 1302
RsaNI GTAC 4 cut(s) 404, 471, 1133, 1301
RseI CAYNNNNRTG 3 cut(s) 69, 1079, 1379
SaqAI TTAA 5 cut(s) 525, 582, 890, 941, 1332
SatI GCNGC 6 cut(s) 39, 58, 148, 151, 546, 877
Sau3AI GATC 9 cut(s) 21, 171, 205, 397, 460, 673, 807, 1199, 1552
Sau96I GGNCC 3 cut(s) 503, 764, 1151
SchI GAGTC 1 cut(s) 168
ScrFI CCNGG 4 cut(s) 31, 282, 576, 1094
SduI GDGCHC 2 cut(s) 8, 570
SfaAI GCGATCGC 1 cut(s) 24
SfaNI GCATC 5 cut(s) 847, 1097, 1255, 1277, 1415
SfcI CTRYAG 2 cut(s) 900, 1244
SfoI GGCGCC 1 cut(s) 286
SgfI GCGATCGC 1 cut(s) 24
SgrAI CRCCGGYG 1 cut(s) 347
SmiMI CAYNNNNRTG 3 cut(s) 69, 1079, 1379
SmlI CTYRAG 3 cut(s) 314, 410, 700
SmoI CTYRAG 3 cut(s) 314, 410, 700
SphI GCATGC 1 cut(s) 20
Sse9I AATT 9 cut(s) 549, 680, 790, 997, 1049, 1107, 1237, 1416, 1482
SsiI CCGC 7 cut(s) 39, 55, 58, 351, 561, 877, 969
SspDI GGCGCC 1 cut(s) 284
SspMI CTAG 2 cut(s) 1098, 1433
StyD4I CCNGG 4 cut(s) 29, 280, 574, 1092
StyI CCWWGG 1 cut(s) 1030
TaaI ACNGT 7 cut(s) 68, 380, 470, 656, 888, 1562, 1577
TaiI ACGT 3 cut(s) 276, 440, 760
TaqI TCGA 6 cut(s) 157, 195, 240, 336, 963, 1564
TasI AATT 9 cut(s) 549, 680, 790, 997, 1049, 1107, 1237, 1416, 1482
TatI WGTACW 2 cut(s) 470, 1300
TauI GCSGC 3 cut(s) 41, 60, 879
TfiI GAWTC 3 cut(s) 1071, 1384, 1519
Tru1I TTAA 5 cut(s) 525, 582, 890, 941, 1332
Tru9I TTAA 5 cut(s) 525, 582, 890, 941, 1332
TscAI CASTG 2 cut(s) 874, 1072
TseFI GTSAC 2 cut(s) 62, 429
TseI GCWGC 3 cut(s) 147, 150, 545
Tsp45I GTSAC 2 cut(s) 62, 429
TspDTI ATGAA 5 cut(s) 482, 713, 1063, 1439, 1597
TspGWI ACGGA 1 cut(s) 82
TspRI CASTG 2 cut(s) 874, 1072
XapI RAATTY 3 cut(s) 997, 1237, 1416
XceI RCATGY 4 cut(s) 20, 561, 848, 1395
XmnI GAANNNNTTC 1 cut(s) 248
XspI CTAG 2 cut(s) 1098, 1433
ZraI GACGTC 1 cut(s) 274
Zsp2I ATGCAT 3 cut(s) 458, 1270, 1408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.