RchiOBHm_Chr2g0111921

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0008632
Physical Location & Seq
Reverse (-)
23650716 .. 23655986
5271 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ48546

Sequence Viewer

Length: 1635 bp
ATGGGCACTCTCTCGCATGCGATCGCGCCCGGGCTCGCCGCCCTCCACCACCACCGCCGCCGTCACGGTAATGGAGTATTCTCCTCCTCCTCCGTCTCTTCTTCTTCTCGAACCTCGCGCTCTTCTTCTTCTTCTGCTTCGCGATTGACGACGAAGAAGAAGAGCAGCAGCGTTCGAGTCTTGGCAGGGATCAAGCACAACAACCAGCGCGACCTCGATTTCAGCGATCCTGACTGGAAATCAAAGTTCCAGAGCGATTTCGAGAAGCGCTTCGCCATCCCTCACATCACCGACGTCTTCCCCGACGCCGTTCCCATTCCCTCCACCTTCTGCCTCAAGATGAGAACTCCGGTCATCGAAGACTTCGCCGGCGGATATCCGTCCGACGAGGAGTGGCACGGTTACATAAATAACAACGATCGCGTACTTCTCAAGACTATATACTACTCGTCACCTACGTCTGCTGGTGCCGAATGCATTGATCCTAACTGTACTTGGGTGGAGCAATGGGTTCATCGTGCTGGGCCACGAGAAAAGATATATTTTAAACCCGAAGAAGTGAAAGCAGCAATTGTAACATGCGGAGGGCTCTGTCCTGGTCTTAATGATGTCATCAGACAGATTGTCATTACACTTGAAATATATGGTGTAAAAAAGATTGTTGGTATTCCTTACGGTTATCGTGGCTTTTCTGATCCAGAATTAGCCGAAATGCCACTCTCAAGGAAGGTGGTTCAAAATGTTCATCTTTCTGGTGGAAGCTTGTTAGGAGTTTCACGTGGAGGCCCCAGCGTTAGTGAAATAGTGGACAATTTAGAGGAAAGAGGGATCAATATGCTCTTTGTTTTGGGTGGAAACGGTACACATGCTGGAGCTGATGCAATACACACTGAGTGCCGCAAAAGACAGTTAAGGGTGGCTATAGTTGGTGTGCCGAAAACCATAGACAATGACATTTTGTTAATGGACAAAACATTTGGTTTCGATACTGCTGTTGAAGAAGCCCAGCGAGCAATAAATTCAGCATATATTGAGGCACATAGTGCTTACCATGGTATTGGCGTTGTGAAATTGATGGGTCGTAGCAGTGGATTCATTGCAATGCACGCATCCCTGGCTAGTGGACAAATTGACATATGTTTGATACCTGAGGTACCTTTTCAAATACATGGCCCTCATGGTATTCTGCACCATCTGAAATACCTGATTGAAACAAAGGGATCAGCTGTGGTCTGTGTAGCAGAGGGAGCAGGGCAGAATTTACTACAGAAAACCAATGCTACTGATGCATCAGGAAATGTTGTATTTGGGGATATTGGAGTACATATTCAACAAGAGACAAAGAAACATTTTAAGGAGCATGGTGTTCCTGTTGATGTGAAATATATAGACCCCACATACATGATTCGTGCATGTCGTGCAAATGCATCAGATGGAATTCTTTGCGCTGTGCTAGGGCAGAATGCTGTTCATGGTGCATTTGCTGGATATAGTGGCATTACAATTGGCATATGCAACACTCATTATGTATATCTTCCGATTCCTGAGGTTATTTCTCATCCCCGACTGGTGGATCCCAACAGTCGAATGTGGCACCGTTGCTTGACTTCAACAGGCCAGCCAGACTTCATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003872 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008443 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009536 GO:0009987 GO:0016052 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019200 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044262 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046835 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0061615 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko00051 ko00052 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04152 ko05230 map00010 map00030 map00051 map00052 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04152 map05230
Pfam Domains
Protein Families
PFK

Protein Analysis

544

Amino Acids

59.25

Weight (kDa)

7.96

Isoelectric Point (pI)

39.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PFK PF00365 189 - 491 7e-62 Phosphofructokinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 297
Acc65I GGTACC 1 cut(s) 1153
AccB1I GGYRCC 3 cut(s) 467, 1153, 1593
AccII CGCG 5 cut(s) 26, 118, 142, 210, 423
AciI CCGC 6 cut(s) 39, 55, 58, 372, 582, 898
AclWI GGATC 8 cut(s) 197, 221, 476, 689, 836, 1228, 1568, 1581
AcsI RAATTY 3 cut(s) 1018, 1258, 1437
AcvI CACGTG 1 cut(s) 779
AcyI GRCGYC 2 cut(s) 294, 306
AdeI CACNNNGTG 2 cut(s) 894, 1043
AfaI GTAC 5 cut(s) 426, 493, 862, 1155, 1323
AfeI AGCGCT 1 cut(s) 269
AfiI CCNNNNNNNGG 1 cut(s) 1570
AgsI TTSAA 7 cut(s) 638, 737, 998, 1163, 1211, 1331, 1611
AhdI GACNNNNNGTC 1 cut(s) 623
AjnI CCWGG 2 cut(s) 595, 1113
AluBI AGCT 3 cut(s) 762, 875, 1226
AluI AGCT 3 cut(s) 762, 875, 1226
Alw26I GTCTC 2 cut(s) 100, 1331
AlwI GGATC 8 cut(s) 197, 221, 476, 689, 836, 1228, 1568, 1581
Ama87I CYCGRG 1 cut(s) 29
Aor51HI AGCGCT 1 cut(s) 269
AoxI GGCC 4 cut(s) 524, 784, 1171, 1615
ApeKI GCWGC 3 cut(s) 165, 168, 566
ApoI RAATTY 3 cut(s) 1018, 1258, 1437
ArsI GACNNNNNNTTYG 2 cut(s) 959, 991
AsiSI GCGATCGC 1 cut(s) 24
Asp700I GAANNNNTTC 1 cut(s) 269
Asp718I GGTACC 1 cut(s) 1153
AspLEI GCGC 5 cut(s) 28, 120, 210, 270, 1448
AspS9I GGNCC 3 cut(s) 524, 785, 1172
AsuC2I CCSGG 2 cut(s) 30, 31
AsuHPI GGTGA 2 cut(s) 280, 444
AvaI CYCGRG 1 cut(s) 29
AxyI CCTNAGG 2 cut(s) 1149, 1545
BaeGI GKGCMC 1 cut(s) 8
BaeI ACNNNNGTAYC 2 cut(s) 978, 1011
BamHI GGATCC 1 cut(s) 1573
BanI GGYRCC 3 cut(s) 467, 1153, 1593
BanII GRGCYC 2 cut(s) 36, 591
BauI CACGAG 1 cut(s) 528
BbrPI CACGTG 1 cut(s) 779
BbsI GAAGAC 2 cut(s) 289, 366
BbvI GCAGC 3 cut(s) 177, 180, 578
BccI CCATC 4 cut(s) 284, 1069, 1200, 1427
BceAI ACGGC 2 cut(s) 45, 293
BciT130I CCWGG 2 cut(s) 597, 1115
BcnI CCSGG 2 cut(s) 30, 31
BcoDI GTCTC 2 cut(s) 100, 1331
BfaI CTAG 2 cut(s) 1119, 1454
BfmI CTRYAG 2 cut(s) 921, 1265
BfoI RGCGCY 1 cut(s) 271
BisI GCNGC 6 cut(s) 39, 58, 166, 169, 567, 898
BlsI GCNGC 6 cut(s) 40, 59, 167, 170, 568, 899
Bme1390I CCNGG 4 cut(s) 30, 31, 597, 1115
BmeRI GACNNNNNGTC 1 cut(s) 623
BmeT110I CYCGRG 1 cut(s) 29
BmgT120I GGNCC 3 cut(s) 524, 785, 1172
BmiI GGNNCC 5 cut(s) 469, 787, 1155, 1575, 1595
BmrFI CCNGG 4 cut(s) 30, 31, 597, 1115
BmsI GCATC 5 cut(s) 868, 1118, 1276, 1298, 1436
BpiI GAAGAC 2 cut(s) 289, 366
BpmI CTGGAG 1 cut(s) 891
BpuEI CTTGAG 3 cut(s) 320, 416, 706
BpuMI CCSGG 2 cut(s) 30, 31
BsaAI YACGTR 1 cut(s) 779
BsaHI GRCGYC 2 cut(s) 294, 306
BsaJI CCNNGG 3 cut(s) 29, 1051, 1113
BsaWI WCCGGW 1 cut(s) 349
Bsc4I CCNNNNNNNGG 1 cut(s) 1570
Bse118I RCCGGY 1 cut(s) 368
Bse1I ACTGG 2 cut(s) 239, 1572
Bse21I CCTNAGG 2 cut(s) 1149, 1545
Bse3DI GCAATG 3 cut(s) 512, 1095, 1107
BseBI CCWGG 2 cut(s) 597, 1115
BseDI CCNNGG 3 cut(s) 29, 1051, 1113
BseGI GGATG 3 cut(s) 276, 1109, 1558
BseLI CCNNNNNNNGG 1 cut(s) 1570
BseMI GCAATG 3 cut(s) 512, 1095, 1107
BseMII CTCAG 3 cut(s) 882, 1140, 1536
BseNI ACTGG 2 cut(s) 239, 1572
BseRI GAGGAG 4 cut(s) 73, 76, 79, 404
BseSI GKGCMC 1 cut(s) 8
BseXI GCAGC 3 cut(s) 177, 180, 578
BseYI CCCAGC 3 cut(s) 521, 788, 1005
BsgI GTGCAG 1 cut(s) 1172
Bsh1236I CGCG 5 cut(s) 26, 118, 142, 210, 423
Bsh1285I CGRYCG 2 cut(s) 24, 421
BshFI GGCC 4 cut(s) 526, 786, 1173, 1617
BshNI GGYRCC 3 cut(s) 467, 1153, 1593
BsiEI CGRYCG 2 cut(s) 24, 421
BsiHKCI CYCGRG 1 cut(s) 29
BsiSI CCGG 3 cut(s) 30, 350, 369
BslI CCNNNNNNNGG 1 cut(s) 1570
BsmAI GTCTC 2 cut(s) 100, 1331
BsmBI CGTCTC 1 cut(s) 100
BsmI GAATGC 2 cut(s) 479, 1468
BsnI GGCC 4 cut(s) 526, 786, 1173, 1617
BsoBI CYCGRG 1 cut(s) 29
Bsp1286I GDGCHC 3 cut(s) 8, 36, 591
Bsp143I GATC 9 cut(s) 21, 189, 226, 418, 481, 694, 828, 1220, 1573
Bsp19I CCATGG 1 cut(s) 1051
Bsp68I TCGCGA 1 cut(s) 142
BspACI CCGC 6 cut(s) 39, 55, 58, 372, 582, 898
BspANI GGCC 4 cut(s) 526, 786, 1173, 1617
BspCNI CTCAG 3 cut(s) 883, 1141, 1537
BspFNI CGCG 5 cut(s) 26, 118, 142, 210, 423
BspLI GGNNCC 5 cut(s) 469, 787, 1155, 1575, 1595
BspPI GGATC 8 cut(s) 197, 221, 476, 689, 836, 1228, 1568, 1581
BspQI GCTCTTC 2 cut(s) 127, 155
BspT107I GGYRCC 3 cut(s) 467, 1153, 1593
BsrDI GCAATG 3 cut(s) 512, 1095, 1107
BsrFI RCCGGY 1 cut(s) 368
BsrI ACTGG 2 cut(s) 239, 1572
BssAI RCCGGY 1 cut(s) 368
BssECI CCNNGG 3 cut(s) 29, 1051, 1113
BssMI GATC 9 cut(s) 21, 189, 226, 418, 481, 694, 828, 1220, 1573
BssNI GRCGYC 2 cut(s) 294, 306
BssSI CACGAG 1 cut(s) 528
BssT1I CCWWGG 1 cut(s) 1051
Bst2BI CACGAG 1 cut(s) 528
Bst2UI CCWGG 2 cut(s) 597, 1115
Bst4CI ACNGT 8 cut(s) 68, 401, 491, 677, 860, 909, 1583, 1598
Bst6I CTCTTC 3 cut(s) 103, 127, 155
BstACI GRCGYC 2 cut(s) 294, 306
BstAPI GCANNNNNTGC 2 cut(s) 1043, 1418
BstBAI YACGTR 1 cut(s) 779
BstC8I GCNNGC 6 cut(s) 18, 36, 370, 1011, 1107, 1619
BstDEI CTNAG 3 cut(s) 891, 1149, 1545
BstDSI CCRYGG 1 cut(s) 1051
BstF5I GGATG 3 cut(s) 276, 1109, 1558
BstFNI CGCG 5 cut(s) 26, 118, 142, 210, 423
BstH2I RGCGCY 1 cut(s) 271
BstHHI GCGC 5 cut(s) 28, 120, 210, 270, 1448
BstKTI GATC 9 cut(s) 24, 192, 229, 421, 484, 697, 831, 1223, 1576
BstMAI GTCTC 2 cut(s) 100, 1331
BstMBI GATC 9 cut(s) 21, 189, 226, 418, 481, 694, 828, 1220, 1573
BstMCI CGRYCG 2 cut(s) 24, 421
BstMWI GCNNNNNNNGC 7 cut(s) 1010, 1043, 1106, 1115, 1247, 1286, 1418
BstNI CCWGG 2 cut(s) 597, 1115
BstNSI RCATGY 4 cut(s) 20, 582, 869, 1416
BstSCI CCNGG 4 cut(s) 28, 29, 595, 1113
BstSFI CTRYAG 2 cut(s) 921, 1265
BstSLI GKGCMC 1 cut(s) 8
BstUI CGCG 5 cut(s) 26, 118, 142, 210, 423
BstV1I GCAGC 3 cut(s) 177, 180, 578
BstV2I GAAGAC 2 cut(s) 289, 366
BstX2I RGATCY 1 cut(s) 1573
BstXI CCANNNNNNTGG 1 cut(s) 1058
BstYI RGATCY 1 cut(s) 1573
Bsu36I CCTNAGG 2 cut(s) 1149, 1545
BsuRI GGCC 4 cut(s) 526, 786, 1173, 1617
BtgI CCRYGG 1 cut(s) 1051
BtsCI GGATG 3 cut(s) 276, 1109, 1558
BtsI GCAGTG 1 cut(s) 1093
BtsIMutI CAGTG 2 cut(s) 888, 1093
BtuMI TCGCGA 1 cut(s) 142
Cac8I GCNNGC 6 cut(s) 18, 36, 370, 1011, 1107, 1619
CfoI GCGC 5 cut(s) 28, 120, 210, 270, 1448
Cfr10I RCCGGY 1 cut(s) 368
Cfr13I GGNCC 3 cut(s) 524, 785, 1172
Cfr9I CCCGGG 1 cut(s) 29
CseI GACGC 1 cut(s) 314
Csp6I GTAC 5 cut(s) 425, 492, 861, 1154, 1322
CspCI CAANNNNNGTGG 2 cut(s) 711, 746
CviQI GTAC 5 cut(s) 425, 492, 861, 1154, 1322
DdeI CTNAG 3 cut(s) 891, 1149, 1545
DpnI GATC 9 cut(s) 23, 191, 228, 420, 483, 696, 830, 1222, 1575
DpnII GATC 9 cut(s) 21, 189, 226, 418, 481, 694, 828, 1220, 1573
DraI TTTAAA 1 cut(s) 547
DraIII CACNNNGTG 2 cut(s) 894, 1043
DriI GACNNNNNGTC 1 cut(s) 623
Eam1104I CTCTTC 3 cut(s) 103, 127, 155
Eam1105I GACNNNNNGTC 1 cut(s) 623
EarI CTCTTC 3 cut(s) 103, 127, 155
EciI GGCGGA 1 cut(s) 387
Eco130I CCWWGG 1 cut(s) 1051
Eco24I GRGCYC 2 cut(s) 36, 591
Eco32I GATATC 1 cut(s) 377
Eco47III AGCGCT 1 cut(s) 269
Eco72I CACGTG 1 cut(s) 779
Eco81I CCTNAGG 2 cut(s) 1149, 1545
Eco88I CYCGRG 1 cut(s) 29
EcoO109I RGGNCCY 1 cut(s) 785
EcoRI GAATTC 1 cut(s) 1437
EcoRII CCWGG 2 cut(s) 595, 1113
EcoRV GATATC 1 cut(s) 377
EcoT14I CCWWGG 1 cut(s) 1051
EcoT22I ATGCAT 3 cut(s) 479, 1291, 1429
EcoT38I GRGCYC 2 cut(s) 36, 591
ErhI CCWWGG 1 cut(s) 1051
Esp3I CGTCTC 1 cut(s) 100
FauNDI CATATG 2 cut(s) 1136, 1511
Fnu4HI GCNGC 6 cut(s) 39, 58, 166, 169, 567, 898
FokI GGATG 3 cut(s) 263, 1096, 1545
FriOI GRGCYC 2 cut(s) 36, 591
Fsp4HI GCNGC 6 cut(s) 39, 58, 166, 169, 567, 898
FspBI CTAG 2 cut(s) 1119, 1454
GlaI GCGC 5 cut(s) 27, 119, 209, 269, 1447
GluI GCNGC 6 cut(s) 39, 58, 166, 169, 567, 898
GsaI CCCAGC 3 cut(s) 525, 792, 1009
GsuI CTGGAG 1 cut(s) 891
HaeII RGCGCY 1 cut(s) 271
HaeIII GGCC 4 cut(s) 526, 786, 1173, 1617
HapII CCGG 3 cut(s) 30, 350, 369
HgaI GACGC 1 cut(s) 314
HhaI GCGC 5 cut(s) 28, 120, 210, 270, 1448
Hin1I GRCGYC 2 cut(s) 294, 306
Hin6I GCGC 5 cut(s) 26, 118, 208, 268, 1446
HinP1I GCGC 5 cut(s) 26, 118, 208, 268, 1446
HindIII AAGCTT 1 cut(s) 760
HinfI GANTC 4 cut(s) 177, 1092, 1405, 1540
HpaII CCGG 3 cut(s) 30, 350, 369
HphI GGTGA 2 cut(s) 280, 444
Hpy166II GTNNAC 3 cut(s) 808, 863, 1124
Hpy188I TCNGA 6 cut(s) 385, 617, 694, 1197, 1432, 1539
Hpy8I GTNNAC 3 cut(s) 808, 863, 1124
Hpy99I CGWCG 4 cut(s) 154, 296, 308, 389
HpyAV CCTTC 2 cut(s) 337, 721
HpyCH4III ACNGT 8 cut(s) 68, 401, 491, 677, 860, 909, 1583, 1598
HpyCH4IV ACGT 3 cut(s) 294, 458, 778
HpyF10VI GCNNNNNNNGC 7 cut(s) 1010, 1043, 1106, 1115, 1247, 1286, 1418
HpyF3I CTNAG 3 cut(s) 891, 1149, 1545
HpySE526I ACGT 3 cut(s) 294, 458, 778
Hsp92I GRCGYC 2 cut(s) 294, 306
HspAI GCGC 5 cut(s) 26, 118, 208, 268, 1446
KpnI GGTACC 1 cut(s) 1157
KroI GCCGGC 1 cut(s) 368
KroNI GCCGGC 1 cut(s) 370
Kzo9I GATC 9 cut(s) 21, 189, 226, 418, 481, 694, 828, 1220, 1573
LguI GCTCTTC 2 cut(s) 127, 155
LmnI GCTCC 4 cut(s) 502, 872, 1247, 1357
Lsp1109I GCAGC 3 cut(s) 177, 180, 578
LweI GCATC 5 cut(s) 868, 1118, 1276, 1298, 1436
MaeI CTAG 2 cut(s) 1119, 1454
MaeII ACGT 3 cut(s) 294, 458, 778
MaeIII GTNAC 4 cut(s) 62, 401, 450, 574
MalI GATC 9 cut(s) 23, 191, 228, 420, 483, 696, 830, 1222, 1575
MboI GATC 9 cut(s) 21, 189, 226, 418, 481, 694, 828, 1220, 1573
MfeI CAATTG 2 cut(s) 570, 1503
MflI RGATCY 1 cut(s) 1573
MhlI GDGCHC 3 cut(s) 8, 36, 591
MluCI AATT 9 cut(s) 570, 701, 811, 1018, 1070, 1128, 1258, 1437, 1503
MlyI GAGTC 1 cut(s) 186
MmeI TCCRAC 1 cut(s) 408
Mph1103I ATGCAT 3 cut(s) 479, 1291, 1429
MreI CGCCGGCG 1 cut(s) 368
MroNI GCCGGC 1 cut(s) 368
MroXI GAANNNNTTC 1 cut(s) 269
MseI TTAA 5 cut(s) 546, 603, 911, 962, 1353
MslI CAYNNNNRTG 3 cut(s) 69, 1100, 1400
MspA1I CMGCKG 1 cut(s) 1226
MspI CCGG 3 cut(s) 30, 350, 369
MspR9I CCNGG 4 cut(s) 30, 31, 597, 1115
MunI CAATTG 2 cut(s) 570, 1503
Mva1269I GAATGC 2 cut(s) 479, 1468
MvaI CCWGG 2 cut(s) 597, 1115
MvnI CGCG 5 cut(s) 26, 118, 142, 210, 423
MwoI GCNNNNNNNGC 7 cut(s) 1010, 1043, 1106, 1115, 1247, 1286, 1418
NaeI GCCGGC 1 cut(s) 370
NciI CCSGG 2 cut(s) 30, 31
NcoI CCATGG 1 cut(s) 1051
NdeI CATATG 2 cut(s) 1136, 1511
NdeII GATC 9 cut(s) 21, 189, 226, 418, 481, 694, 828, 1220, 1573
NgoMIV GCCGGC 1 cut(s) 368
NlaIV GGNNCC 5 cut(s) 469, 787, 1155, 1575, 1595
NmuCI GTSAC 2 cut(s) 62, 450
NruI TCGCGA 1 cut(s) 142
NsiI ATGCAT 3 cut(s) 479, 1291, 1429
NspI RCATGY 4 cut(s) 20, 582, 869, 1416
PaeI GCATGC 1 cut(s) 20
PciSI GCTCTTC 2 cut(s) 127, 155
PcsI WCGNNNNNNNCGW 5 cut(s) 146, 222, 300, 455, 1414
PctI GAATGC 2 cut(s) 479, 1468
PdiI GCCGGC 1 cut(s) 370
PdmI GAANNNNTTC 1 cut(s) 269
PfeI GAWTC 3 cut(s) 1092, 1405, 1540
PkrI GCNGC 6 cut(s) 40, 59, 167, 170, 568, 899
Ple19I CGATCG 2 cut(s) 24, 421
PleI GAGTC 1 cut(s) 185
PmaCI CACGTG 1 cut(s) 779
PmlI CACGTG 1 cut(s) 779
PpsI GAGTC 1 cut(s) 185
Ppu21I YACGTR 1 cut(s) 779
Psp6I CCWGG 2 cut(s) 595, 1113
PspCI CACGTG 1 cut(s) 779
PspFI CCCAGC 3 cut(s) 521, 788, 1005
PspGI CCWGG 2 cut(s) 595, 1113
PspN4I GGNNCC 5 cut(s) 469, 787, 1155, 1575, 1595
PspPI GGNCC 3 cut(s) 524, 785, 1172
PsuI RGATCY 1 cut(s) 1573
PvuI CGATCG 2 cut(s) 24, 421
PvuII CAGCTG 1 cut(s) 1226
RgaI GCGATCGC 1 cut(s) 24
RruI TCGCGA 1 cut(s) 142
RsaI GTAC 5 cut(s) 426, 493, 862, 1155, 1323
RsaNI GTAC 5 cut(s) 425, 492, 861, 1154, 1322
RseI CAYNNNNRTG 3 cut(s) 69, 1100, 1400
SapI GCTCTTC 2 cut(s) 127, 155
SaqAI TTAA 5 cut(s) 546, 603, 911, 962, 1353
SatI GCNGC 6 cut(s) 39, 58, 166, 169, 567, 898
Sau3AI GATC 9 cut(s) 21, 189, 226, 418, 481, 694, 828, 1220, 1573
Sau96I GGNCC 3 cut(s) 524, 785, 1172
SchI GAGTC 1 cut(s) 186
ScrFI CCNGG 4 cut(s) 30, 31, 597, 1115
SduI GDGCHC 3 cut(s) 8, 36, 591
SfaAI GCGATCGC 1 cut(s) 24
SfaNI GCATC 5 cut(s) 868, 1118, 1276, 1298, 1436
SfcI CTRYAG 2 cut(s) 921, 1265
SgfI GCGATCGC 1 cut(s) 24
SgrAI CRCCGGYG 1 cut(s) 368
SmaI CCCGGG 1 cut(s) 31
SmiMI CAYNNNNRTG 3 cut(s) 69, 1100, 1400
SmlI CTYRAG 3 cut(s) 335, 431, 721
SmoI CTYRAG 3 cut(s) 335, 431, 721
SphI GCATGC 1 cut(s) 20
SrfI GCCCGGGC 1 cut(s) 31
Sse9I AATT 9 cut(s) 570, 701, 811, 1018, 1070, 1128, 1258, 1437, 1503
SsiI CCGC 6 cut(s) 39, 55, 58, 372, 582, 898
SspMI CTAG 2 cut(s) 1119, 1454
StyD4I CCNGG 4 cut(s) 28, 29, 595, 1113
StyI CCWWGG 1 cut(s) 1051
TaaI ACNGT 8 cut(s) 68, 401, 491, 677, 860, 909, 1583, 1598
TaiI ACGT 3 cut(s) 297, 461, 781
TaqI TCGA 7 cut(s) 109, 175, 216, 261, 357, 984, 1585
TasI AATT 9 cut(s) 570, 701, 811, 1018, 1070, 1128, 1258, 1437, 1503
TatI WGTACW 2 cut(s) 491, 1321
TauI GCSGC 3 cut(s) 41, 60, 900
TfiI GAWTC 3 cut(s) 1092, 1405, 1540
Tru1I TTAA 5 cut(s) 546, 603, 911, 962, 1353
Tru9I TTAA 5 cut(s) 546, 603, 911, 962, 1353
TscAI CASTG 2 cut(s) 895, 1093
TseFI GTSAC 2 cut(s) 62, 450
TseI GCWGC 3 cut(s) 165, 168, 566
Tsp45I GTSAC 2 cut(s) 62, 450
TspDTI ATGAA 5 cut(s) 503, 734, 1084, 1460, 1618
TspGWI ACGGA 2 cut(s) 82, 369
TspMI CCCGGG 1 cut(s) 29
TspRI CASTG 2 cut(s) 895, 1093
XapI RAATTY 3 cut(s) 1018, 1258, 1437
XceI RCATGY 4 cut(s) 20, 582, 869, 1416
XmaI CCCGGG 1 cut(s) 29
XmnI GAANNNNTTC 1 cut(s) 269
XspI CTAG 2 cut(s) 1119, 1454
ZraI GACGTC 1 cut(s) 295
Zsp2I ATGCAT 3 cut(s) 479, 1291, 1429
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.