AT2G25140

Belongs to the ClpA ClpB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0009882
Physical Location & Seq
Reverse (-)
10697629 .. 10702203
4575 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G25140.1

Sequence Viewer

Length: 2895 bp
ATGGCGCTAAGGAGATTATCAAAGTCTGTTTCGTCAGCCATCAAAGCTCAATACACTCTCTCGAGACCCTCTCCTCTCCTCCGTTCTCGCTCTCTCTCTTCCTCTCCTCACTACACCTCTATTGGCCGGCCAACAAATTCATTCATCGGAAAAATCAATAACTCTTCTATCACTCATGCGACTACTACTCATGGTCAACTCTTCCCTCTTTCTTCTCCGCGACGCTTTTGTACTACCACAGCTCAGGTTAATCAAAATGAGTTTACGGAGATGGCATGGGAAGGGCTTATCAACGCCTTTGACGCGGCTCGTGAATCGAAACAACAGATTGTGGAATCTGAACATTTGATGAAAGCTCTTCTTGAGCAAAAAGATGGTATGGCTAGAAAGATTTTCACTAAAGCTGGAATTGACAATTCCTCTGTTCTTCAAGCTACAGACTTGTTCATATCTAAACAACCAACGGTTAGTGATGCAAGTGGTCAGAGACTAGGGTCATCTCTTAGTGTTATATTAGAAAATGCAAAGAGGCATAAGAAAGATATGTTGGATAGTTATGTATCCGTGGAGCACTTTTTGTTGGCTTATTATTCAGATACAAGGTTTGGACAAGAGTTTTTCAGGGACATGAAACTTGATATTCAAGTGCTAAAGGATGCTATTAAAGATGTACGCGGTGATCAACGAGTAACTGATCGGAATCCTGAAAGCAAATATCAAGCACTTGAAAAGTATGGAAATGATTTAACTGAGATGGCTAGACGAGGGAAGCTGGATCCTGTCATTGGGCGGGATGATGAAATTAGGCGTTGCATCCAGATATTATGTAGGAGAACAAAAAACAATCCGGTTATTATTGGTGAACCTGGTGTAGGGAAAACTGCAATTGCAGAGGGGTTGGCTCAACGAATTGTGCGAGGAGATGTCCCTGAACCTCTCATGAATCGAAAGTTGATATCTCTCGACATGGGTTCACTTCTTGCTGGTGCCAAATTTCGAGGAGATTTTGAGGAGAGGTTGAAAGCAGTAATGAAGGAAGTATCTGCTTCCAATGGGCAAACAATCTTGTTTATTGATGAGATCCACACTGTTGTTGGTGCAGGAGCTATGGATGGAGCAATGGATGCAAGCAACCTCTTGAAACCAATGCTTGGACGAGGTGAACTGAGATGCATTGGTGCAACTACACTGACCGAATACCGCAAATATATAGAGAAAGACCCAGCTCTAGAGCGTAGGTTTCAACAAGTGTTATGTGTTCAGCCCTCTGTTGAAGATACAATCTCGATTCTTCGTGGGTTAAGAGAGCGGTACGAGTTACACCATGGCGTTACTATATCAGATAGTGCCCTTGTTTCAGCAGCAGTTCTTGCAGACCGCTACATCACCGAACGCTTTCTGCCAGACAAAGCTATTGATCTTGTTGACGAAGCTGGTGCAAAGCTGAAGATGGAGATCACTTCTAAACCAACTGAACTAGATGGAATAGATAGAGCTGTGATCAAATTGGAGATGGAAAAGCTTTCTCTTAAAAATGATACTGATAAAGCATCTAAAGAACGGCTACAGAAGATTGAGAATGATCTGTCCACGCTCAAACAGAAACAGAAAGAACTCAATGTCCAATGGGAGAAAGAAAAGTCGCTCATGACAAAAATACGTTCGTTTAAGGAAGAGATTGATCGAGTTAACCTCGAAATTGAATCTGCTGAACGTGAATATGATCTAAACCGAGCTGCTGAGCTGAAGTATGGAACACTTTTGTCCCTCCAACGCCAATTAGAAGAAGCTGAGAAGAATCTCACGAATTTCCGACAGTTTGGACAGTCACTACTCCGAGAAGTGGTTACCGATCTTGACATAGCAGAAATAGTAAGCAAGTGGACTGGTATTCCACTATCTAATCTTCAGCAATCAGAGAGAGAGAAGTTAGTGATGTTGGAAGAAGTGCTCCACCATAGAGTTATTGGTCAAGACATGGCTGTAAAATCAGTAGCAGATGCTATCCGACGTTCAAGGGCTGGTCTTTCTGATCCTAACCGCCCAATAGCTAGTTTCATGTTCATGGGTCCAACAGGTGTTGGTAAGACCGAGCTTGCCAAGGCTCTAGCGGGATACCTTTTTAACACTGAAAATGCAATAGTGAGGGTCGATATGAGTGAGTACATGGAAAAACACTCAGTCTCACGGCTTGTTGGTGCACCACCTGGCTATGTCGGTTATGAAGAAGGTGGGCAGTTAACTGAAGTTGTTCGCAGGAGACCTTACTCGGTGGTTCTGTTTGATGAGATCGAGAAAGCCCACCCTGATGTCTTTAATATCTTGCTACAACTGCTAGACGATGGAAGAATAACTGATTCTCAAGGAAGGACCGTAAGTTTCAAAAACTGTGTTGTAATTATGACGTCGAATATAGGATCTCACCACATACTTGAGACCCTCCGCAACAATGAAGACAGCAAAGAAGCAGTTTATGAGATAATGAAGCGGCAAGTTGTGGAGTTAGCAAGACAAAATTTCAGGCCTGAGTTCATGAACAGAATTGACGAGTACATAGTTTTCCAGCCGCTGGATTCTAACGAAATCTCCAAAATCGTAGAGTTACAGATGAGACGGGTGAAGAATAGCTTGGAACAGAAGAAAATCAAACTTCAGTATACCAAAGAGGCAGTGGATCTCCTTGCTCAGCTTGGCTTTGACCCTAACTACGGGGCAAGGCCGGTGAAGCGAGTGATTCAACAGATGGTGGAGAATGAGATCGCTGTCGGGATTTTGAAAGGAGATTTTGCAGAAGAAGATACCGTTCTTGTCGATGTTGACCACTTGGCTAGTGACAACAAATTGGTTATCAAGAAGCTTGAGAGTAATGCTTCAGCTGAAGAAATGGCCGCTTAA

Protein Analysis

964

Amino Acids

108.66

Weight (kDa)

6.54

Isoelectric Point (pI)

40.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clp_N PF02861 87 - 203 1.6e-32 Clp repeat (R) N-terminal domain
NBD_SMAX1 PF23569 266 - 365 9.3e-07 SMAX1 nucleotide binding domain
AAA PF00004 285 - 419 1.3e-13 ATPase family associated with various cellular activities (AAA)
AAA_lid_9 PF17871 423 - 525 4.3e-34 AAA lid domain
AAA_2 PF07724 681 - 850 3.2e-69 AAA domain (Cdc48 subfamily)
AAA_5 PF07728 686 - 807 3.7e-12 AAA domain (dynein-related subfamily)
AAA PF00004 687 - 815 8.4e-10 ATPase family associated with various cellular activities (AAA)
ClpB_D2-small PF10431 857 - 937 8.1e-25 C-terminal, D2-small domain, of ClpB protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0009882)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G25140
fragaria_vesca FvH4_2g40970 FvH4_2g40970 FvH4_2g40970 FvH4_2g40970
malus_domestica MD08G1058100.v1.1 MD15G1038200.v1.1
prunus_persica Prupe.1G392200_v2.0.a1
pyrus_communis pycom08g04820 pycom15g03540
rosa_chinensis RchiOBHm_Chr6g0299601
rosa_laevigata RLG00000011406
rosa_multiflora Rmu_sc0002895.1_g000001
rosa_roxburghii Rroxscaffold_7G00168520
rosa_rugosa Rorug06G0290800 Rorug06G0290900
rosa_samantha Rh6AG401700 Rh6BG410100 Rh6CG415700 Rh6DG402900
rosa_wichuraiana Rw6G035150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2408
AccB1I GGYRCC 1 cut(s) 986
AccB7I CCANNNNNTGG 2 cut(s) 1151, 2569
AccBSI CCGCTC 1 cut(s) 1309
AccI GTMKAC 1 cut(s) 2657
AccII CGCG 3 cut(s) 220, 305, 675
AclWI GGATC 6 cut(s) 770, 783, 1075, 2027, 2425, 2682
AcoI YGGCCR 3 cut(s) 124, 128, 2886
AcsI RAATTY 4 cut(s) 136, 992, 1807, 2515
AcuI CTGAAG 6 cut(s) 1466, 1766, 1892, 2265, 2636, 2856
AcyI GRCGYC 1 cut(s) 2405
AfaI GTAC 5 cut(s) 232, 672, 1313, 2165, 2552
AfiI CCNNNNNNNGG 6 cut(s) 785, 872, 1151, 1843, 2569, 2708
AjnI CCWGG 2 cut(s) 865, 2206
AjuI GAANNNNNNNTTGG 4 cut(s) 530, 562, 2612, 2644
AloI GAACNNNNNNTCC 2 cut(s) 1605, 1637
Alw21I GWGCWC 3 cut(s) 573, 1953, 2203
Alw26I GTCTC 6 cut(s) 58, 481, 2188, 2254, 2429, 2605
Alw44I GTGCAC 1 cut(s) 2199
AlwI GGATC 6 cut(s) 770, 783, 1075, 2027, 2425, 2682
AlwNI CAGNNNCTG 1 cut(s) 2569
Ama87I CYCGRG 1 cut(s) 61
AoxI GGCC 5 cut(s) 124, 128, 2522, 2717, 2886
ApaLI GTGCAC 1 cut(s) 2199
ApeKI GCWGC 2 cut(s) 1361, 1736
ApoI RAATTY 4 cut(s) 136, 992, 1807, 2515
Asp700I GAANNNNTTC 2 cut(s) 1395, 2250
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 2 cut(s) 2069, 2370
AsuHPI GGTGA 7 cut(s) 689, 872, 1172, 1378, 2414, 2629, 2734
AvaI CYCGRG 1 cut(s) 61
AvaII GGWCC 2 cut(s) 2069, 2370
BaeGI GKGCMC 2 cut(s) 1351, 2203
BamHI GGATCC 1 cut(s) 775
BanI GGYRCC 1 cut(s) 986
BauI CACGAG 1 cut(s) 309
BbsI GAAGAC 1 cut(s) 2460
Bbv12I GWGCWC 3 cut(s) 573, 1953, 2203
BbvI GCAGC 2 cut(s) 1373, 1723
BceAI ACGGC 2 cut(s) 1577, 2204
BcgI CGANNNNNNTGC 2 cut(s) 1418, 1452
BciT130I CCWGG 2 cut(s) 867, 2208
BciVI GTATCC 2 cut(s) 571, 2108
BclI TGATCA 2 cut(s) 679, 1500
BcoDI GTCTC 6 cut(s) 58, 481, 2188, 2254, 2429, 2605
BfaI CTAG 9 cut(s) 384, 491, 759, 1229, 1478, 2052, 2108, 2336, 2829
BfmI CTRYAG 2 cut(s) 435, 1565
BfoI RGCGCY 1 cut(s) 8
BfuI GTATCC 2 cut(s) 571, 2108
BisI GCNGC 6 cut(s) 306, 1362, 1737, 2489, 2567, 2889
BlpI GCTNAGC 2 cut(s) 1740, 2685
BlsI GCNGC 6 cut(s) 307, 1363, 1738, 2490, 2568, 2890
Bme1390I CCNGG 2 cut(s) 867, 2208
Bme18I GGWCC 2 cut(s) 2069, 2370
BmeT110I CYCGRG 1 cut(s) 61
BmgT120I GGNCC 2 cut(s) 2069, 2370
BmiI GGNNCC 3 cut(s) 777, 988, 2070
BmrFI CCNGG 2 cut(s) 867, 2208
BmsI GCATC 7 cut(s) 463, 646, 822, 1114, 1160, 1559, 1990
BoxI GACNNNNGTC 1 cut(s) 493
BpiI GAAGAC 1 cut(s) 2460
BplI GAGNNNNNCTC 4 cut(s) 55, 87, 1677, 1709
Bpu10I CCTNAGC 2 cut(s) 8, 243
Bpu1102I GCTNAGC 2 cut(s) 1740, 2685
BpuEI CTTGAG 4 cut(s) 383, 2346, 2453, 2879
BsaBI GATNNNNATC 2 cut(s) 699, 1454
BsaHI GRCGYC 1 cut(s) 2405
BsaI GGTCTC 3 cut(s) 58, 2254, 2429
BsaJI CCNNGG 3 cut(s) 564, 1324, 2100
BsaWI WCCGGW 1 cut(s) 847
BsaXI ACNNNNNCTCC 2 cut(s) 2570, 2600
Bsc4I CCNNNNNNNGG 6 cut(s) 785, 872, 1151, 1843, 2569, 2708
Bse118I RCCGGY 2 cut(s) 126, 2719
Bse1I ACTGG 1 cut(s) 1891
Bse3DI GCAATG 1 cut(s) 1125
Bse8I GATNNNNATC 2 cut(s) 699, 1454
BseBI CCWGG 2 cut(s) 867, 2208
BseDI CCNNGG 3 cut(s) 564, 1324, 2100
BseGI GGATG 5 cut(s) 661, 799, 813, 1117, 1129
BseJI GATNNNNATC 2 cut(s) 699, 1454
BseLI CCNNNNNNNGG 6 cut(s) 785, 872, 1151, 1843, 2569, 2708
BseMI GCAATG 1 cut(s) 1125
BseMII CTCAG 8 cut(s) 257, 741, 1157, 1731, 1782, 2193, 2517, 2699
BseNI ACTGG 1 cut(s) 1891
BseRI GAGGAG 6 cut(s) 63, 68, 96, 933, 1014, 1025
BseSI GKGCMC 2 cut(s) 1351, 2203
BseXI GCAGC 2 cut(s) 1373, 1723
BseYI CCCAGC 1 cut(s) 1222
BsgI GTGCAG 1 cut(s) 1119
Bsh1236I CGCG 3 cut(s) 220, 305, 675
BshFI GGCC 5 cut(s) 126, 130, 2524, 2719, 2888
BshNI GGYRCC 1 cut(s) 986
BsiHKAI GWGCWC 3 cut(s) 573, 1953, 2203
BsiHKCI CYCGRG 1 cut(s) 61
BsiSI CCGG 3 cut(s) 127, 848, 2720
BslFI GGGAC 3 cut(s) 638, 911, 1750
BslI CCNNNNNNNGG 6 cut(s) 785, 872, 1151, 1843, 2569, 2708
BsmAI GTCTC 6 cut(s) 58, 481, 2188, 2254, 2429, 2605
BsmBI CGTCTC 1 cut(s) 2605
BsmFI GGGAC 3 cut(s) 638, 911, 1750
BsnI GGCC 5 cut(s) 126, 130, 2524, 2719, 2888
Bso31I GGTCTC 3 cut(s) 58, 2254, 2429
BsoBI CYCGRG 1 cut(s) 61
Bsp1286I GDGCHC 4 cut(s) 573, 1351, 1953, 2203
Bsp1720I GCTNAGC 2 cut(s) 1740, 2685
Bsp19I CCATGG 1 cut(s) 1324
BspANI GGCC 5 cut(s) 126, 130, 2524, 2719, 2888
BspCNI CTCAG 8 cut(s) 256, 742, 1158, 1732, 1783, 2192, 2518, 2698
BspFNI CGCG 3 cut(s) 220, 305, 675
BspHI TCATGA 3 cut(s) 939, 1647, 2532
BspLI GGNNCC 3 cut(s) 777, 988, 2070
BspPI GGATC 6 cut(s) 770, 783, 1075, 2027, 2425, 2682
BspQI GCTCTTC 1 cut(s) 363
BspT107I GGYRCC 1 cut(s) 986
BspTNI GGTCTC 3 cut(s) 58, 2254, 2429
BsrBI CCGCTC 1 cut(s) 1309
BsrDI GCAATG 1 cut(s) 1125
BsrFI RCCGGY 2 cut(s) 126, 2719
BsrI ACTGG 1 cut(s) 1891
BssAI RCCGGY 2 cut(s) 126, 2719
BssECI CCNNGG 3 cut(s) 564, 1324, 2100
BssNAI GTATAC 1 cut(s) 2658
BssNI GRCGYC 1 cut(s) 2405
BssSI CACGAG 1 cut(s) 309
BssT1I CCWWGG 2 cut(s) 1324, 2100
Bst1107I GTATAC 1 cut(s) 2658
Bst2BI CACGAG 1 cut(s) 309
Bst2UI CCWGG 2 cut(s) 867, 2208
Bst4CI ACNGT 7 cut(s) 466, 1090, 1818, 1827, 2374, 2390, 2803
Bst6I CTCTTC 5 cut(s) 103, 169, 206, 363, 1668
BstACI GRCGYC 1 cut(s) 2405
BstAPI GCANNNNNTGC 2 cut(s) 1124, 1370
BstC8I GCNNGC 3 cut(s) 128, 1129, 2097
BstDSI CCRYGG 2 cut(s) 564, 1324
BstEII GGTNACC 1 cut(s) 1846
BstENI CCTNNNNNAGG 1 cut(s) 870
BstF5I GGATG 5 cut(s) 661, 799, 813, 1117, 1129
BstFNI CGCG 3 cut(s) 220, 305, 675
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstMAI GTCTC 6 cut(s) 58, 481, 2188, 2254, 2429, 2605
BstMWI GCNNNNNNNGC 6 cut(s) 44, 302, 1124, 1370, 2332, 2725
BstNI CCWGG 2 cut(s) 867, 2208
BstPAI GACNNNNGTC 1 cut(s) 493
BstPI GGTNACC 1 cut(s) 1846
BstSCI CCNGG 2 cut(s) 865, 2206
BstSFI CTRYAG 2 cut(s) 435, 1565
BstSLI GKGCMC 2 cut(s) 1351, 2203
BstUI CGCG 3 cut(s) 220, 305, 675
BstV1I GCAGC 2 cut(s) 1373, 1723
BstV2I GAAGAC 1 cut(s) 2460
BstX2I RGATCY 4 cut(s) 775, 1080, 2417, 2674
BstYI RGATCY 4 cut(s) 775, 1080, 2417, 2674
BstZ17I GTATAC 1 cut(s) 2658
BsuI GTATCC 2 cut(s) 571, 2108
BsuRI GGCC 5 cut(s) 126, 130, 2524, 2719, 2888
BtgI CCRYGG 2 cut(s) 564, 1324
BtsCI GGATG 5 cut(s) 661, 799, 813, 1117, 1129
BtsI GCAGTG 1 cut(s) 2676
BtsIMutI CAGTG 4 cut(s) 1086, 1187, 2127, 2676
Cac8I GCNNGC 3 cut(s) 128, 1129, 2097
CaiI CAGNNNCTG 1 cut(s) 2569
CciI TCATGA 3 cut(s) 939, 1647, 2532
CfoI GCGC 1 cut(s) 7
Cfr10I RCCGGY 2 cut(s) 126, 2719
Cfr13I GGNCC 2 cut(s) 2069, 2370
CseI GACGC 2 cut(s) 231, 311
CsiI ACCWGGT 1 cut(s) 865
Csp6I GTAC 5 cut(s) 231, 671, 1312, 2164, 2551
CviQI GTAC 5 cut(s) 231, 671, 1312, 2164, 2551
EaeI YGGCCR 3 cut(s) 124, 128, 2886
Eam1104I CTCTTC 5 cut(s) 103, 169, 206, 363, 1668
EarI CTCTTC 5 cut(s) 103, 169, 206, 363, 1668
Eco130I CCWWGG 2 cut(s) 1324, 2100
Eco147I AGGCCT 1 cut(s) 2524
Eco31I GGTCTC 3 cut(s) 58, 2254, 2429
Eco32I GATATC 1 cut(s) 957
Eco47I GGWCC 2 cut(s) 2069, 2370
Eco57I CTGAAG 6 cut(s) 1466, 1766, 1892, 2265, 2636, 2856
Eco88I CYCGRG 1 cut(s) 61
Eco91I GGTNACC 1 cut(s) 1846
EcoNI CCTNNNNNAGG 1 cut(s) 870
EcoO65I GGTNACC 1 cut(s) 1846
EcoRII CCWGG 2 cut(s) 865, 2206
EcoRV GATATC 1 cut(s) 957
EcoT14I CCWWGG 2 cut(s) 1324, 2100
EcoT22I ATGCAT 1 cut(s) 1175
ErhI CCWWGG 2 cut(s) 1324, 2100
Esp3I CGTCTC 1 cut(s) 2605
FalI AAGNNNNNCTT 4 cut(s) 345, 377, 2612, 2644
FaqI GGGAC 3 cut(s) 638, 911, 1750
FauI CCCGC 2 cut(s) 783, 2104
FbaI TGATCA 2 cut(s) 679, 1500
FblI GTMKAC 1 cut(s) 2657
Fnu4HI GCNGC 6 cut(s) 306, 1362, 1737, 2489, 2567, 2889
FokI GGATG 5 cut(s) 668, 800, 806, 1124, 1136
FseI GGCCGGCC 1 cut(s) 130
Fsp4HI GCNGC 6 cut(s) 306, 1362, 1737, 2489, 2567, 2889
FspBI CTAG 9 cut(s) 384, 491, 759, 1229, 1478, 2052, 2108, 2336, 2829
GlaI GCGC 1 cut(s) 6
GluI GCNGC 6 cut(s) 306, 1362, 1737, 2489, 2567, 2889
GsaI CCCAGC 1 cut(s) 1226
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 5 cut(s) 126, 130, 2524, 2719, 2888
HapII CCGG 3 cut(s) 127, 848, 2720
HgaI GACGC 2 cut(s) 231, 311
HhaI GCGC 1 cut(s) 7
Hin1I GRCGYC 1 cut(s) 2405
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HincII GTYRAC 5 cut(s) 197, 1426, 1690, 2241, 2818
HindII GTYRAC 5 cut(s) 197, 1426, 1690, 2241, 2818
HindIII AAGCTT 2 cut(s) 1520, 2855
HpaI GTTAAC 2 cut(s) 1690, 2241
HpaII CCGG 3 cut(s) 127, 848, 2720
HphI GGTGA 7 cut(s) 689, 872, 1172, 1378, 2414, 2629, 2734
Hpy99I CGWCG 3 cut(s) 225, 2013, 2410
HpyAV CCTTC 4 cut(s) 275, 1027, 2222, 2361
HpyCH4III ACNGT 7 cut(s) 466, 1090, 1818, 1827, 2374, 2390, 2803
HpyCH4IV ACGT 4 cut(s) 1660, 1714, 2011, 2405
HpyF10VI GCNNNNNNNGC 6 cut(s) 44, 302, 1124, 1370, 2332, 2725
HpySE526I ACGT 4 cut(s) 1660, 1714, 2011, 2405
Hsp92I GRCGYC 1 cut(s) 2405
HspAI GCGC 1 cut(s) 5
KroI GCCGGC 1 cut(s) 126
KroNI GCCGGC 1 cut(s) 128
Ksp22I TGATCA 2 cut(s) 679, 1500
KspAI GTTAAC 2 cut(s) 1690, 2241
LguI GCTCTTC 1 cut(s) 363
LmnI GCTCC 4 cut(s) 568, 1103, 1115, 1956
Lsp1109I GCAGC 2 cut(s) 1373, 1723
LweI GCATC 7 cut(s) 463, 646, 822, 1114, 1160, 1559, 1990
MabI ACCWGGT 1 cut(s) 865
MaeI CTAG 9 cut(s) 384, 491, 759, 1229, 1478, 2052, 2108, 2336, 2829
MaeII ACGT 4 cut(s) 1660, 1714, 2011, 2405
MaeIII GTNAC 7 cut(s) 688, 1317, 1330, 1827, 1846, 2601, 2831
MbiI CCGCTC 1 cut(s) 1309
MfeI CAATTG 1 cut(s) 885
MflI RGATCY 4 cut(s) 775, 1080, 2417, 2674
MhlI GDGCHC 4 cut(s) 573, 1351, 1953, 2203
MmeI TCCRAC 6 cut(s) 528, 1795, 1837, 1920, 2032, 2096
Mph1103I ATGCAT 1 cut(s) 1175
MroNI GCCGGC 1 cut(s) 126
MroXI GAANNNNTTC 2 cut(s) 1395, 2250
MslI CAYNNNNRTG 2 cut(s) 2063, 2307
MspA1I CMGCKG 2 cut(s) 2569, 2876
MspI CCGG 3 cut(s) 127, 848, 2720
MspR9I CCNGG 2 cut(s) 867, 2208
MunI CAATTG 1 cut(s) 885
MvaI CCWGG 2 cut(s) 867, 2208
MvnI CGCG 3 cut(s) 220, 305, 675
MwoI GCNNNNNNNGC 6 cut(s) 44, 302, 1124, 1370, 2332, 2725
NaeI GCCGGC 1 cut(s) 128
NcoI CCATGG 1 cut(s) 1324
NgoMIV GCCGGC 1 cut(s) 126
NlaIV GGNNCC 3 cut(s) 777, 988, 2070
NmuCI GTSAC 2 cut(s) 1827, 2831
NsiI ATGCAT 1 cut(s) 1175
PaeR7I CTCGAG 1 cut(s) 61
PagI TCATGA 3 cut(s) 939, 1647, 2532
PceI AGGCCT 1 cut(s) 2524
PciSI GCTCTTC 1 cut(s) 363
PcsI WCGNNNNNNNCGW 1 cut(s) 913
PdiI GCCGGC 1 cut(s) 128
PdmI GAANNNNTTC 2 cut(s) 1395, 2250
PflMI CCANNNNNTGG 2 cut(s) 1151, 2569
PkrI GCNGC 6 cut(s) 307, 1363, 1738, 2490, 2568, 2890
PshAI GACNNNNGTC 1 cut(s) 493
Psp6I CCWGG 2 cut(s) 865, 2206
PspEI GGTNACC 1 cut(s) 1846
PspFI CCCAGC 1 cut(s) 1222
PspGI CCWGG 2 cut(s) 865, 2206
PspN4I GGNNCC 3 cut(s) 777, 988, 2070
PspPI GGNCC 2 cut(s) 2069, 2370
PstNI CAGNNNCTG 1 cut(s) 2569
PsuI RGATCY 4 cut(s) 775, 1080, 2417, 2674
PvuII CAGCTG 1 cut(s) 2876
RigI GGCCGGCC 1 cut(s) 130
RsaI GTAC 5 cut(s) 232, 672, 1313, 2165, 2552
RsaNI GTAC 5 cut(s) 231, 671, 1312, 2164, 2551
RseI CAYNNNNRTG 2 cut(s) 2063, 2307
SapI GCTCTTC 1 cut(s) 363
SatI GCNGC 6 cut(s) 306, 1362, 1737, 2489, 2567, 2889
Sau96I GGNCC 2 cut(s) 2069, 2370
ScrFI CCNGG 2 cut(s) 867, 2208
SduI GDGCHC 4 cut(s) 573, 1351, 1953, 2203
SexAI ACCWGGT 1 cut(s) 865
SfaNI GCATC 7 cut(s) 463, 646, 822, 1114, 1160, 1559, 1990
SfcI CTRYAG 2 cut(s) 435, 1565
Sfr274I CTCGAG 1 cut(s) 61
SinI GGWCC 2 cut(s) 2069, 2370
SlaI CTCGAG 1 cut(s) 61
SmiMI CAYNNNNRTG 2 cut(s) 2063, 2307
SmlI CTYRAG 5 cut(s) 61, 362, 2361, 2432, 2858
SmoI CTYRAG 5 cut(s) 61, 362, 2361, 2432, 2858
SseBI AGGCCT 1 cut(s) 2524
SspMI CTAG 9 cut(s) 384, 491, 759, 1229, 1478, 2052, 2108, 2336, 2829
StuI AGGCCT 1 cut(s) 2524
StyD4I CCNGG 2 cut(s) 865, 2206
StyI CCWWGG 2 cut(s) 1324, 2100
TaaI ACNGT 7 cut(s) 466, 1090, 1818, 1827, 2374, 2390, 2803
TaiI ACGT 4 cut(s) 1663, 1717, 2014, 2408
TaqII GACCGA 2 cut(s) 1208, 2105
TatI WGTACW 3 cut(s) 230, 2163, 2550
TauI GCSGC 4 cut(s) 308, 2491, 2569, 2891
TscAI CASTG 4 cut(s) 1093, 1194, 2134, 2676
TseFI GTSAC 2 cut(s) 1827, 2831
TseI GCWGC 2 cut(s) 1361, 1736
Tsp45I GTSAC 2 cut(s) 1827, 2831
TspGWI ACGGA 3 cut(s) 71, 281, 553
TspRI CASTG 4 cut(s) 1093, 1194, 2134, 2676
Van91I CCANNNNNTGG 2 cut(s) 1151, 2569
VneI GTGCAC 1 cut(s) 2199
VpaK11BI GGWCC 2 cut(s) 2069, 2370
XagI CCTNNNNNAGG 1 cut(s) 870
XapI RAATTY 4 cut(s) 136, 992, 1807, 2515
XbaI TCTAGA 1 cut(s) 1228
XcmI CCANNNNNNNNNTGG 3 cut(s) 1091, 1964, 2668
XhoI CTCGAG 1 cut(s) 61
XmiI GTMKAC 1 cut(s) 2657
XmnI GAANNNNTTC 2 cut(s) 1395, 2250
XspI CTAG 9 cut(s) 384, 491, 759, 1229, 1478, 2052, 2108, 2336, 2829
ZraI GACGTC 1 cut(s) 2406
Zsp2I ATGCAT 1 cut(s) 1175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.