Rh6DG402900

Belongs to the ClpA ClpB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0009882
Physical Location & Seq
Reverse (-)
59147932 .. 59152554
4623 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG402900.1

Sequence Viewer

Length: 2919 bp
ATGGCTAGCCGGCAAGCGACGAAGCTCAGCAAATCGGCGATGGTTTTGGCGGCCATGAACGGCGCCTCCAAAACCTCAAGAAGTTCGCTCTCTCGGTGTCGTGAAACTGCGGTGGCTGCCTCCAAGTTTTCCATGGGCGGCTCCGTCAATGTCGTTTCGGCCAAGTATGTGGCCAACAGTCTCAGTCGGAGCTTTCACTCTTCGGGTCCTCGTTTCAACTCCGCCAGCAGCGCTGCTCAGGCTCAGCAGAATGAGTTCACTGAGATGGCATGGGAGGGCATAATTGGGGCTGTGGAAGCCGCCAGAAATAGCAAGCAACAAGTGGTGGAATCTGAGCACCTGATGAAAGCTGTCTTGGAGCAGAAGGATGGTTTGGCCAGGAGAATCTTCACCAAGGCTGGTATAGACAACACCACTGTTCTCCAGGCCACCGATGACTTCATTGCTCAGCAACCCAAGGTGATGGGTGCGACCAGTGGACCTATAATAGGCACACATCTCGGCGCTCTGTTGGAGAATGCCCGAAGGCAGAAGAAAGAAATGAATGATGATTTTGTGTCTGTGGAGCACCTTGTGTTGGCGTTTCAGTCCGACACACGGTTTGGGCAGCAATTGTTCAAAAACCTCCAGCTTACTGACAAGGATTTGAAGGAGGCTGTTAAACATGTTCGTGGAAATCAGAGAGTCACTGATCAAAATCCTGAAGGGAAATATGAGGCTTTGACTAAATATGGGAACGACTTGACTGAGCTCGCCAGTCGTGGCAAACTTGATCCAGTTATTGGACGGGATGATGAAATACGGCGTTGTATCCAAATACTGTCCAGGAGAACAAAAAATAATCCTGTTATTATTGGAGAGCCTGGTGTCGGAAAAACTGCAATCGCTGAAGGATTAGCTCAACGGATTGTGCGTGGGGATGTTCCGGAACCCCTATTGAACAGAAAGTTGATTTCTTTAGATATGGGTTCATTGGTCGCTGGAGCCAAGTTTCGCGGTGACTTTGAGGAAAGATTGAAAGCTGTATTGAAGGAAGTTACTGCTTCAAATGGACAGATCATATTATTCATTGATGAGATTCATACTGTTGTAGGAGCAGGGGCTACAAGTGGTGCAATGGATGCTGGGAACTTGTTGAAACCAATGCTTGGGCGGGGTGAACTACGGTGTATAGGAGCAACCACTTTGAATGAGTACAGAAAATACATTGAGAAGGATCCTGCACTGGAGCGTAGGTTTCAGCAGGTTTTTTGTGGTCAGCCATCTGTCGAAGACACGATATCCATTCTTCGTGGGCTGCGTGAGCGGTACGAGCTGCATCATGGTGTGAAAATATCAGATAGTGCCCTTGTTTCTGCAGCAGTTCTTTCTGACAGATACATTACAGAACGATTTTTGCCTGACAAAGCCATTGATCTTGTTGATGAAGCTGCTGCAAAGCTGAAAATGGAGATCACATCCAAGCCAACTGAGTTAGATGAGGTAGACAGAGCTGTACTGAAGTTGGAGATGGAGAAGCTATCTTTGCAAAATGACACAGATAAATCATCAAAAGAAAGGTTAAGCAAGCTGGAGAGTGACCTCGCATTGCTCAAACAGAAACAAAAAGAATTTAATGAACAGTGGGATCGTGAGAAGGCTCTCATGACTCGAATACGATCAATTAAAGAAGAGATTGATAGAGTAAACCAAGAGATGGAAGCTGCTGAACGTGCATATGACCTAAGTCGTGCTGCTGAGCTCAAATATGGAACTCTAATGTCCCTGCAACGCCAGTTAGAAGAGGCTGAGAAAAACCTTTCTGAGTACCAGAAGTCTGGAAAGTCTTTCCTACGAGAAGAAGTCACCGATCTTGATATTGCTGAGATTGTAAGCAAATGGACTGGTATACCTTTGTCAAACCTTCAACAGTCGGAAAGAGACAAGCTAGTCATGCTAGAACAGGTACTTCATAAGAGGGTAGTGGGTCAAGATATAGCCGTGAAATCGGTAGCTGATGCAATCAGACGTTCAAGGGCTGGTCTATCAGACCCAAACCGACCTATTGCAAGTTTCATGTTTTTGGGACCTACTGGTGTGGGGAAAACAGAGCTTGCAAAGGCCTTGGCCAGCTATCTCTTCAACACAGAAAATGCTCTGGTTAGGATTGATATGAGTGAGTACATGGAAAAGCATGCAGTTTCACGTTTGGTGGGTGCTCCGCCTGGTTATGTTGGTTATGAAGAAGGTGGACAACTAACAGAAGTGGTTCGTCGAAGACCCTATTGTGTAGTGCTATTCGATGAAATAGAGAAAGCACATCAGGATGTGTTCAATATTTTGTTACAATTGCTGGATGATGGAAGGATAACCGACTCACAAGGAAGGACTGTAAGCTTCACCAATTGTGTTGTGATAATGACCTCAAATCTTGGCTCCCACTATATACTTGAAACCCTTCGTAATACTCAAGACAGCAAGGATGCAGTTTATGAGTTGATGAAAAGACAAGTGGTTGAATTGGCAAGACAGACTTTCCGCCCTGAGTTTTTGAATCGAGTTGATGAGTTTATCGTGTTCCAGCCTTTGGACTCCAAAGAGATCTGTAGCATTGTTGACATACAGATGAACCGGGTTAAAGATAGGCTGAAGCAAAAGAAAATCGAGCTTCATTATACAAAGGAAGCTGTGGAGCTTCTTGGTAAATTGGGCTTTGATCCGAACTTTGGAGCAAGGCCAGTAAAGAGGGTGATACAGCAGCTGGTTGAGAATGAAATTGCAATGGGACTGTTGAGAGGCGATTTCAGTGAGGAGGATGCGATCATTGTTGATGCTGAGGTGTCCCCATCCGCCAAAGATATTCCTCCACAGAAGAGGTTGCGCATCAGGAAATTGGAGAATGCTTCTTCTAGTGTAGATGACATGGTTGCGAATGACTGA

Protein Analysis

972

Amino Acids

108.5

Weight (kDa)

6.74

Isoelectric Point (pI)

36.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clp_N PF02861 85 - 208 2.1e-28 Clp repeat (R) N-terminal domain
NBD_SMAX1 PF23569 265 - 365 1.2e-06 SMAX1 nucleotide binding domain
AAA PF00004 284 - 415 8.1e-13 ATPase family associated with various cellular activities (AAA)
AAA_lid_9 PF17871 422 - 524 5.8e-35 AAA lid domain
AAA_2 PF07724 680 - 849 6.7e-69 AAA domain (Cdc48 subfamily)
AAA_5 PF07728 685 - 806 3.4e-12 AAA domain (dynein-related subfamily)
AAA PF00004 686 - 808 3.1e-10 ATPase family associated with various cellular activities (AAA)
ClpB_D2-small PF10431 856 - 936 1.7e-23 C-terminal, D2-small domain, of ClpB protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0009882)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G25140
fragaria_vesca FvH4_2g40970 FvH4_2g40970 FvH4_2g40970 FvH4_2g40970
malus_domestica MD08G1058100.v1.1 MD15G1038200.v1.1
prunus_persica Prupe.1G392200_v2.0.a1
pyrus_communis pycom08g04820 pycom15g03540
rosa_chinensis RchiOBHm_Chr6g0299601
rosa_laevigata RLG00000011406
rosa_multiflora Rmu_sc0002895.1_g000001
rosa_roxburghii Rroxscaffold_7G00168520
rosa_rugosa Rorug06G0290800 Rorug06G0290900
rosa_samantha Rh6AG401700 Rh6BG410100 Rh6CG415700 Rh6DG402900
rosa_wichuraiana Rw6G035150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1928
Acc16I TGCGCA 1 cut(s) 2861
Acc36I ACCTGC 1 cut(s) 1234
AccB1I GGYRCC 1 cut(s) 62
AccB7I CCANNNNNTGG 4 cut(s) 782, 1148, 1696, 2566
AccBSI CCGCTC 1 cut(s) 1306
AccI GTMKAC 2 cut(s) 1485, 1888
AccII CGCG 1 cut(s) 996
AccIII TCCGGA 1 cut(s) 925
AclWI GGATC 5 cut(s) 767, 1211, 1224, 1635, 2690
AcoI YGGCCR 5 cut(s) 51, 159, 171, 375, 2106
AcsI RAATTY 1 cut(s) 1610
AcuI CTGAAG 4 cut(s) 723, 909, 1520, 2648
AcyI GRCGYC 1 cut(s) 63
AdeI CACNNNGTG 1 cut(s) 574
AfaI GTAC 6 cut(s) 1196, 1310, 1497, 1808, 1947, 2162
AfeI AGCGCT 1 cut(s) 232
AfiI CCNNNNNNNGG 9 cut(s) 488, 577, 597, 782, 869, 1148, 1696, 2566, 2705
AflIII ACRYGT 1 cut(s) 664
AjnI CCWGG 5 cut(s) 377, 423, 824, 862, 2203
AjuI GAANNNNNNNTTGG 4 cut(s) 338, 356, 370, 388
AloI GAACNNNNNNTCC 2 cut(s) 50, 82
Alw21I GWGCWC 5 cut(s) 339, 570, 753, 1743, 2200
Alw26I GTCTC 2 cut(s) 185, 1914
AlwI GGATC 5 cut(s) 767, 1211, 1224, 1635, 2690
AlwNI CAGNNNCTG 1 cut(s) 2740
Aor13HI TCCGGA 1 cut(s) 925
Aor51HI AGCGCT 1 cut(s) 232
AoxI GGCC 8 cut(s) 51, 159, 171, 375, 426, 2100, 2106, 2714
ApoI RAATTY 1 cut(s) 1610
ArsI GACNNNNNNTTYG 2 cut(s) 584, 616
Asp700I GAANNNNTTC 3 cut(s) 254, 2247, 2436
AspLEI GCGC 4 cut(s) 65, 233, 506, 2862
AspS9I GGNCC 3 cut(s) 206, 479, 2066
AsuC2I CCSGG 1 cut(s) 2612
AsuHPI GGTGA 7 cut(s) 382, 472, 1010, 1169, 1837, 2371, 2740
AsuNHI GCTAGC 1 cut(s) 5
AvaII GGWCC 3 cut(s) 206, 479, 2066
BaeGI GKGCMC 1 cut(s) 1348
BalI TGGCCA 3 cut(s) 173, 377, 2108
BamHI GGATCC 1 cut(s) 1216
BanI GGYRCC 1 cut(s) 62
BanII GRGCYC 2 cut(s) 753, 1743
BbsI GAAGAC 2 cut(s) 1278, 2263
Bbv12I GWGCWC 5 cut(s) 339, 570, 753, 1743, 2200
BbvCI CCTCAGC 1 cut(s) 2814
BccI CCATC 9 cut(s) 34, 259, 362, 457, 1270, 1504, 1690, 2333, 2833
BceAI ACGGC 3 cut(s) 76, 818, 1964
BcgI CGANNNNNNTGC 2 cut(s) 481, 515
BciT130I CCWGG 5 cut(s) 379, 425, 826, 864, 2205
BciVI GTATCC 1 cut(s) 821
BclI TGATCA 1 cut(s) 691
BcnI CCSGG 1 cut(s) 2612
BcoDI GTCTC 2 cut(s) 185, 1914
BfaI CTAG 4 cut(s) 6, 1928, 1937, 2889
BfmI CTRYAG 2 cut(s) 1356, 2584
BfoI RGCGCY 3 cut(s) 66, 234, 507
BfuAI ACCTGC 1 cut(s) 1234
BfuI GTATCC 1 cut(s) 821
BglII AGATCT 1 cut(s) 2580
BlpI GCTNAGC 4 cut(s) 26, 243, 447, 1737
Bme1390I CCNGG 6 cut(s) 379, 425, 826, 864, 2205, 2612
Bme18I GGWCC 3 cut(s) 206, 479, 2066
BmgT120I GGNCC 3 cut(s) 206, 479, 2066
BmiI GGNNCC 8 cut(s) 64, 142, 207, 930, 985, 1218, 2067, 2416
BmrFI CCNGG 6 cut(s) 379, 425, 826, 864, 2205, 2612
BmsI GCATC 7 cut(s) 1111, 1327, 1987, 2452, 2785, 2800, 2871
BmtI GCTAGC 1 cut(s) 9
BoxI GACNNNNGTC 1 cut(s) 1725
BpiI GAAGAC 2 cut(s) 1278, 2263
BpmI CTGGAG 5 cut(s) 407, 611, 1002, 1247, 1592
Bpu10I CCTNAGC 2 cut(s) 237, 2814
Bpu1102I GCTNAGC 4 cut(s) 26, 243, 447, 1737
BpuEI CTTGAG 2 cut(s) 61, 2433
BpuMI CCSGG 1 cut(s) 2612
BsaBI GATNNNNATC 1 cut(s) 696
BsaHI GRCGYC 1 cut(s) 63
BsaJI CCNNGG 4 cut(s) 132, 393, 456, 2103
BsaWI WCCGGW 1 cut(s) 925
BsaXI ACNNNNNCTCC 4 cut(s) 50, 80, 1220, 1250
Bsc4I CCNNNNNNNGG 9 cut(s) 488, 577, 597, 782, 869, 1148, 1696, 2566, 2705
Bse118I RCCGGY 1 cut(s) 9
Bse1I ACTGG 8 cut(s) 474, 756, 776, 1230, 1774, 1888, 2077, 2717
Bse3DI GCAATG 4 cut(s) 441, 1122, 1586, 2766
Bse8I GATNNNNATC 1 cut(s) 696
BseAI TCCGGA 1 cut(s) 925
BseBI CCWGG 5 cut(s) 379, 425, 826, 864, 2205
BseDI CCNNGG 4 cut(s) 132, 393, 456, 2103
BseJI GATNNNNATC 1 cut(s) 696
BseLI CCNNNNNNNGG 9 cut(s) 488, 577, 597, 782, 869, 1148, 1696, 2566, 2705
BseMI GCAATG 4 cut(s) 441, 1122, 1586, 2766
BseNI ACTGG 8 cut(s) 474, 756, 776, 1230, 1774, 1888, 2077, 2717
BseRI GAGGAG 1 cut(s) 2804
BseSI GKGCMC 1 cut(s) 1348
BseYI CCCAGC 1 cut(s) 1124
BsgI GTGCAG 1 cut(s) 1206
Bsh1236I CGCG 1 cut(s) 996
BshFI GGCC 8 cut(s) 53, 161, 173, 377, 428, 2102, 2108, 2716
BshNI GGYRCC 1 cut(s) 62
BsiHKAI GWGCWC 5 cut(s) 339, 570, 753, 1743, 2200
BsiSI CCGG 3 cut(s) 10, 926, 2611
BslFI GGGAC 4 cut(s) 1747, 2079, 2778, 2806
BslI CCNNNNNNNGG 9 cut(s) 488, 577, 597, 782, 869, 1148, 1696, 2566, 2705
BsmAI GTCTC 2 cut(s) 185, 1914
BsmFI GGGAC 4 cut(s) 1747, 2079, 2778, 2806
BsmI GAATGC 2 cut(s) 523, 2884
BsnI GGCC 8 cut(s) 53, 161, 173, 377, 428, 2102, 2108, 2716
Bsp1286I GDGCHC 6 cut(s) 339, 570, 753, 1348, 1743, 2200
Bsp13I TCCGGA 1 cut(s) 925
Bsp1720I GCTNAGC 4 cut(s) 26, 243, 447, 1737
Bsp19I CCATGG 1 cut(s) 132
BspANI GGCC 8 cut(s) 53, 161, 173, 377, 428, 2102, 2108, 2716
BspEI TCCGGA 1 cut(s) 925
BspFNI CGCG 1 cut(s) 996
BspHI TCATGA 1 cut(s) 1644
BspLI GGNNCC 8 cut(s) 64, 142, 207, 930, 985, 1218, 2067, 2416
BspMAI CTGCAG 1 cut(s) 1360
BspMI ACCTGC 1 cut(s) 1234
BspOI GCTAGC 1 cut(s) 9
BspPI GGATC 5 cut(s) 767, 1211, 1224, 1635, 2690
BspT107I GGYRCC 1 cut(s) 62
BsrBI CCGCTC 1 cut(s) 1306
BsrDI GCAATG 4 cut(s) 441, 1122, 1586, 2766
BsrFI RCCGGY 1 cut(s) 9
BsrI ACTGG 8 cut(s) 474, 756, 776, 1230, 1774, 1888, 2077, 2717
BssAI RCCGGY 1 cut(s) 9
BssECI CCNNGG 4 cut(s) 132, 393, 456, 2103
BssNAI GTATAC 1 cut(s) 1889
BssNI GRCGYC 1 cut(s) 63
BssT1I CCWWGG 4 cut(s) 132, 393, 456, 2103
Bst1107I GTATAC 1 cut(s) 1889
Bst2UI CCWGG 5 cut(s) 379, 425, 826, 864, 2205
Bst6I CTCTTC 5 cut(s) 205, 1665, 1776, 2123, 2846
BstACI GRCGYC 1 cut(s) 63
BstAPI GCANNNNNTGC 1 cut(s) 1121
BstDSI CCRYGG 1 cut(s) 132
BstENI CCTNNNNNAGG 1 cut(s) 486
BstFNI CGCG 1 cut(s) 996
BstH2I RGCGCY 3 cut(s) 66, 234, 507
BstHHI GCGC 4 cut(s) 65, 233, 506, 2862
BstMAI GTCTC 2 cut(s) 185, 1914
BstNI CCWGG 5 cut(s) 379, 425, 826, 864, 2205
BstNSI RCATGY 2 cut(s) 668, 2177
BstPAI GACNNNNGTC 1 cut(s) 1725
BstSCI CCNGG 6 cut(s) 377, 423, 824, 862, 2203, 2610
BstSFI CTRYAG 2 cut(s) 1356, 2584
BstSLI GKGCMC 1 cut(s) 1348
BstUI CGCG 1 cut(s) 996
BstV2I GAAGAC 2 cut(s) 1278, 2263
BstX2I RGATCY 2 cut(s) 1216, 2580
BstXI CCANNNNNNTGG 3 cut(s) 169, 463, 1817
BstYI RGATCY 2 cut(s) 1216, 2580
BstZ17I GTATAC 1 cut(s) 1889
BsuI GTATCC 1 cut(s) 821
BsuRI GGCC 8 cut(s) 53, 161, 173, 377, 428, 2102, 2108, 2716
BtgI CCRYGG 1 cut(s) 132
BtgZI GCGATG 1 cut(s) 53
BtsIMutI CAGTG 7 cut(s) 258, 414, 481, 687, 1223, 1628, 2791
BveI ACCTGC 1 cut(s) 1234
CaiI CAGNNNCTG 1 cut(s) 2740
CciI TCATGA 1 cut(s) 1644
CfoI GCGC 4 cut(s) 65, 233, 506, 2862
Cfr10I RCCGGY 1 cut(s) 9
Cfr13I GGNCC 3 cut(s) 206, 479, 2066
Csp6I GTAC 6 cut(s) 1195, 1309, 1496, 1807, 1946, 2161
CviQI GTAC 6 cut(s) 1195, 1309, 1496, 1807, 1946, 2161
DinI GGCGCC 1 cut(s) 64
DraIII CACNNNGTG 1 cut(s) 574
DrdI GACNNNNNNGTC 1 cut(s) 1928
DseDI GACNNNNNNGTC 1 cut(s) 1928
EaeI YGGCCR 5 cut(s) 51, 159, 171, 375, 2106
Eam1104I CTCTTC 5 cut(s) 205, 1665, 1776, 2123, 2846
EarI CTCTTC 5 cut(s) 205, 1665, 1776, 2123, 2846
EciI GGCGGA 4 cut(s) 211, 2190, 2507, 2818
Ecl136II GAGCTC 2 cut(s) 751, 1741
Eco130I CCWWGG 4 cut(s) 132, 393, 456, 2103
Eco147I AGGCCT 1 cut(s) 2102
Eco24I GRGCYC 2 cut(s) 753, 1743
Eco32I GATATC 1 cut(s) 1281
Eco47I GGWCC 3 cut(s) 206, 479, 2066
Eco47III AGCGCT 1 cut(s) 232
Eco53kI GAGCTC 2 cut(s) 751, 1741
Eco57I CTGAAG 4 cut(s) 723, 909, 1520, 2648
EcoICRI GAGCTC 2 cut(s) 751, 1741
EcoNI CCTNNNNNAGG 1 cut(s) 486
EcoO109I RGGNCCY 2 cut(s) 206, 2066
EcoRII CCWGG 5 cut(s) 377, 423, 824, 862, 2203
EcoRV GATATC 1 cut(s) 1281
EcoT14I CCWWGG 4 cut(s) 132, 393, 456, 2103
EcoT38I GRGCYC 2 cut(s) 753, 1743
EgeI GGCGCC 1 cut(s) 64
EheI GGCGCC 1 cut(s) 64
ErhI CCWWGG 4 cut(s) 132, 393, 456, 2103
FalI AAGNNNNNCTT 2 cut(s) 2497, 2529
FaqI GGGAC 4 cut(s) 1747, 2079, 2778, 2806
FauI CCCGC 1 cut(s) 1146
FauNDI CATATG 1 cut(s) 1717
FbaI TGATCA 1 cut(s) 691
FblI GTMKAC 2 cut(s) 1485, 1888
FriOI GRGCYC 2 cut(s) 753, 1743
FspBI CTAG 4 cut(s) 6, 1928, 1937, 2889
FspI TGCGCA 1 cut(s) 2861
GlaI GCGC 4 cut(s) 64, 232, 505, 2861
GsaI CCCAGC 1 cut(s) 1128
GsuI CTGGAG 5 cut(s) 407, 611, 1002, 1247, 1592
HaeII RGCGCY 3 cut(s) 66, 234, 507
HaeIII GGCC 8 cut(s) 53, 161, 173, 377, 428, 2102, 2108, 2716
HapII CCGG 3 cut(s) 10, 926, 2611
HhaI GCGC 4 cut(s) 65, 233, 506, 2862
Hin1I GRCGYC 1 cut(s) 63
Hin6I GCGC 4 cut(s) 63, 231, 504, 2860
HinP1I GCGC 4 cut(s) 63, 231, 504, 2860
HincII GTYRAC 1 cut(s) 2596
HindII GTYRAC 1 cut(s) 2596
HindIII AAGCTT 1 cut(s) 2374
HinfI GANTC 8 cut(s) 329, 384, 684, 1078, 1648, 2354, 2533, 2570
HpaII CCGG 3 cut(s) 10, 926, 2611
HphI GGTGA 7 cut(s) 382, 472, 1010, 1169, 1837, 2371, 2740
Hpy166II GTNNAC 8 cut(s) 258, 479, 1160, 1486, 1687, 1889, 2231, 2596
Hpy8I GTNNAC 8 cut(s) 258, 479, 1160, 1486, 1687, 1889, 2231, 2596
Hpy99I CGWCG 2 cut(s) 22, 2256
HpyCH4IV ACGT 3 cut(s) 1711, 2008, 2185
HpySE526I ACGT 3 cut(s) 1711, 2008, 2185
Hsp92I GRCGYC 1 cut(s) 63
HspAI GCGC 4 cut(s) 63, 231, 504, 2860
KasI GGCGCC 1 cut(s) 62
Kpn2I TCCGGA 1 cut(s) 925
KroI GCCGGC 1 cut(s) 9
KroNI GCCGGC 1 cut(s) 11
Ksp22I TGATCA 1 cut(s) 691
LweI GCATC 7 cut(s) 1111, 1327, 1987, 2452, 2785, 2800, 2871
MaeI CTAG 4 cut(s) 6, 1928, 1937, 2889
MaeII ACGT 3 cut(s) 1711, 2008, 2185
MaeIII GTNAC 6 cut(s) 685, 998, 1036, 1577, 1843, 2322
MbiI CCGCTC 1 cut(s) 1306
MfeI CAATTG 3 cut(s) 611, 2327, 2383
MflI RGATCY 2 cut(s) 1216, 2580
MhlI GDGCHC 6 cut(s) 339, 570, 753, 1348, 1743, 2200
MlsI TGGCCA 3 cut(s) 173, 377, 2108
MluNI TGGCCA 3 cut(s) 173, 377, 2108
Mly113I GGCGCC 1 cut(s) 63
MlyI GAGTC 4 cut(s) 693, 1642, 2348, 2564
MmeI TCCRAC 6 cut(s) 167, 492, 615, 850, 1485, 1893
Mox20I TGGCCA 3 cut(s) 173, 377, 2108
MroI TCCGGA 1 cut(s) 925
MroNI GCCGGC 1 cut(s) 9
MroXI GAANNNNTTC 3 cut(s) 254, 2247, 2436
MscI TGGCCA 3 cut(s) 173, 377, 2108
MseI TTAA 5 cut(s) 660, 1562, 1614, 1665, 2616
MslI CAYNNNNRTG 5 cut(s) 263, 669, 1323, 2304, 2603
Msp20I TGGCCA 3 cut(s) 173, 377, 2108
MspA1I CMGCKG 1 cut(s) 2740
MspI CCGG 3 cut(s) 10, 926, 2611
MspR9I CCNGG 6 cut(s) 379, 425, 826, 864, 2205, 2612
MunI CAATTG 3 cut(s) 611, 2327, 2383
Mva1269I GAATGC 2 cut(s) 523, 2884
MvaI CCWGG 5 cut(s) 379, 425, 826, 864, 2205
MvnI CGCG 1 cut(s) 996
NaeI GCCGGC 1 cut(s) 11
NarI GGCGCC 1 cut(s) 63
NciI CCSGG 1 cut(s) 2612
NcoI CCATGG 1 cut(s) 132
NdeI CATATG 1 cut(s) 1717
NgoMIV GCCGGC 1 cut(s) 9
NheI GCTAGC 1 cut(s) 5
NlaIV GGNNCC 8 cut(s) 64, 142, 207, 930, 985, 1218, 2067, 2416
NmeAIII GCCGAG 1 cut(s) 480
NmuCI GTSAC 4 cut(s) 685, 998, 1577, 1843
NsbI TGCGCA 1 cut(s) 2861
NspI RCATGY 2 cut(s) 668, 2177
PaeI GCATGC 1 cut(s) 2177
PagI TCATGA 1 cut(s) 1644
PceI AGGCCT 1 cut(s) 2102
PciI ACATGT 1 cut(s) 664
PcsI WCGNNNNNNNCGW 2 cut(s) 910, 1297
PctI GAATGC 2 cut(s) 523, 2884
PdiI GCCGGC 1 cut(s) 11
PdmI GAANNNNTTC 3 cut(s) 254, 2247, 2436
PfeI GAWTC 4 cut(s) 329, 384, 1078, 2533
PflMI CCANNNNNTGG 4 cut(s) 782, 1148, 1696, 2566
PfoI TCCNGGA 1 cut(s) 824
PleI GAGTC 4 cut(s) 692, 1642, 2348, 2564
PluTI GGCGCC 1 cut(s) 66
PpsI GAGTC 4 cut(s) 692, 1642, 2348, 2564
PpuMI RGGWCCY 2 cut(s) 206, 2066
PscI ACATGT 1 cut(s) 664
PshAI GACNNNNGTC 1 cut(s) 1725
Psp124BI GAGCTC 2 cut(s) 753, 1743
Psp5II RGGWCCY 2 cut(s) 206, 2066
Psp6I CCWGG 5 cut(s) 377, 423, 824, 862, 2203
PspFI CCCAGC 1 cut(s) 1124
PspGI CCWGG 5 cut(s) 377, 423, 824, 862, 2203
PspN4I GGNNCC 8 cut(s) 64, 142, 207, 930, 985, 1218, 2067, 2416
PspPI GGNCC 3 cut(s) 206, 479, 2066
PspPPI RGGWCCY 2 cut(s) 206, 2066
PstI CTGCAG 1 cut(s) 1360
PstNI CAGNNNCTG 1 cut(s) 2740
PsuI RGATCY 2 cut(s) 1216, 2580
PvuII CAGCTG 1 cut(s) 2740
RsaI GTAC 6 cut(s) 1196, 1310, 1497, 1808, 1947, 2162
RsaNI GTAC 6 cut(s) 1195, 1309, 1496, 1807, 1946, 2161
RseI CAYNNNNRTG 5 cut(s) 263, 669, 1323, 2304, 2603
SacI GAGCTC 2 cut(s) 753, 1743
SaqAI TTAA 5 cut(s) 660, 1562, 1614, 1665, 2616
Sau96I GGNCC 3 cut(s) 206, 479, 2066
SchI GAGTC 4 cut(s) 693, 1642, 2348, 2564
ScrFI CCNGG 6 cut(s) 379, 425, 826, 864, 2205, 2612
SduI GDGCHC 6 cut(s) 339, 570, 753, 1348, 1743, 2200
SfaNI GCATC 7 cut(s) 1111, 1327, 1987, 2452, 2785, 2800, 2871
SfcI CTRYAG 2 cut(s) 1356, 2584
SfoI GGCGCC 1 cut(s) 64
SinI GGWCC 3 cut(s) 206, 479, 2066
SmiMI CAYNNNNRTG 5 cut(s) 263, 669, 1323, 2304, 2603
SmlI CTYRAG 2 cut(s) 76, 2448
SmoI CTYRAG 2 cut(s) 76, 2448
SphI GCATGC 1 cut(s) 2177
SseBI AGGCCT 1 cut(s) 2102
SspDI GGCGCC 1 cut(s) 62
SspI AATATT 1 cut(s) 2317
SspMI CTAG 4 cut(s) 6, 1928, 1937, 2889
SstI GAGCTC 2 cut(s) 753, 1743
StuI AGGCCT 1 cut(s) 2102
StyD4I CCNGG 6 cut(s) 377, 423, 824, 862, 2203, 2610
StyI CCWWGG 4 cut(s) 132, 393, 456, 2103
TaiI ACGT 3 cut(s) 1714, 2011, 2188
TaqI TCGA 6 cut(s) 1269, 1651, 2254, 2280, 2536, 2643
TatI WGTACW 3 cut(s) 1194, 1495, 2160
TauI GCSGC 3 cut(s) 53, 141, 302
TfiI GAWTC 4 cut(s) 329, 384, 1078, 2533
Tru1I TTAA 5 cut(s) 660, 1562, 1614, 1665, 2616
Tru9I TTAA 5 cut(s) 660, 1562, 1614, 1665, 2616
TscAI CASTG 7 cut(s) 265, 421, 481, 694, 1230, 1628, 2791
TseFI GTSAC 4 cut(s) 685, 998, 1577, 1843
Tsp45I GTSAC 4 cut(s) 685, 998, 1577, 1843
TspGWI ACGGA 2 cut(s) 133, 919
TspRI CASTG 7 cut(s) 265, 421, 481, 694, 1230, 1628, 2791
Van91I CCANNNNNTGG 4 cut(s) 782, 1148, 1696, 2566
VpaK11BI GGWCC 3 cut(s) 206, 479, 2066
XagI CCTNNNNNAGG 1 cut(s) 486
XapI RAATTY 1 cut(s) 1610
XceI RCATGY 2 cut(s) 668, 2177
XcmI CCANNNNNNNNNTGG 1 cut(s) 130
XmiI GTMKAC 2 cut(s) 1485, 1888
XmnI GAANNNNTTC 3 cut(s) 254, 2247, 2436
XspI CTAG 4 cut(s) 6, 1928, 1937, 2889
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.