Rw6G035150

Belongs to the ClpA ClpB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0009882
Physical Location & Seq
Reverse (-)
59737980 .. 59743129
5150 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G035150.1

Sequence Viewer

Length: 2949 bp
ATGGCTAGCCGGCAAGCGACGAAGCTCAGCAAATCGGCGATGGTTTTGGCGGCCATGAACGGCGCCTCCAAAACCTCAACAAGTTCCCTCTCTCGGTGTCGTGAAACTGCGGTGGCTGCCTCCAAGTTTTCCATGGGCGGCTCCGTGGCTCCCTTCAATGTCGTTTGGGATCGAAATCATGTCGTTTCGGCCAAGTATGTGGCAAACACTCTCAGTCGGAGCTTTCACTCTTCGGGTCCTCGTTTCAACTCCGCCAGCAGCGCTGCTCAGGCTCAGCAGAATGAGTTCACTGAGATGGCATGGGAGGGCATAATTGGGGCTGTGGAAGCCGCCAGAAATAGCAAGCAACAAGTGGTGGAATCTGAGCACTTGATGAAAGCTATCCTGGAGCAGAAGGATGGTTTGGCCAGGAGAATCTTCACCAAGGCTGGTGTAGACAACACCACTGTTCTGCAGGCCACCGATGACTTCATTGCTCAGCAACCCAAGGTGATGGGTGCGACCAGTGGACCTATAATAGGCACACATCTCGGCGCTCTGTTGGAGAATGCCCGAAGGCAGAAGAAAGAAATGAATGATGATTTTGTGTCTGTGGAGCACCTTGTGTTGGCGTTTCAGTCCGACACACGGTTTGGCCAGCAATTGTTCAAAAACCTCCAGCTTACTGACAAGGATTTGAAGGAGGCTGTTAAACATGTTCGTGGAAATCAGAGAGTCACCGATCAAAATCCTGAAGGGAAATATGAGGCTTTGACTAAATATGGGAACGACTTGACTGAGCTCGCCAGTCGTGGCAAACTTGATCCAGTTATTGGACGGGATGATGAAATACGCCGTTGTATCCAAATACTGTCCAGGAGAACAAAAAATAATCCCGTTATTATTGGAGAGCCTGGTGTCGGAAAAACTGCAATCGCTGAAGGATTAGCTCAACGGATTGTGCGTGGGGATGTTCCGGAACCCCTATTGAACAGAAAGTTGATTTCTTTAGATATGGGTTCATTGGTCGCTGGAGCCAAGTTTCGTGGTGACTTTGAGGAAAGATTGAAAGCTGTATTGAAGGAAGTTACTGCTTCAAATGGACAGATCATATTATTCATTGATGAGATTCATACTGTTGTAGGAGCAGGGGCTACAAGTGGTGCAATGGATGCTGGGAACTTGTTGAAACCAATGCTTGGGCGGGGTGAACTACGGTGTATAGGAGCAACCACGTTGAATGAGTACAGAAAATACATTGAGAAGGATCCTGCACTGGAGCGTAGGTTTCAGCAGGTTTTTTGTGGTCAGCCATCTGTCGAAGACACGATATCCATTCTTCGTGGGTTGCGTGAGCGGTACGAGCTGCATCATGGTGTGAAAATATCAGATAGTGCCCTTGTTTCTGCAGCAGTTCTTTCTGACAGATACATTACAGAACGATTTTTGCCTGACAAAGCCATTGATCTTGTTGATGAAGCTGCTGCAAAGCTGAAAATGGAGATCACATCCAAGCCAACTGAGTTAGATGAGGTAGACAGAGCTGTACTGAAGTTGGAGATGGAGAAGCTATCTTTGCAAAATGACACAGATAAATCATCGAAAGAAAGGTTAAGCAAGCTGGAGAGTGACCTCGCATTGCTCAAACAGAAACAAAAAGAATTTAATGAACAGTGGGATCTTGAGAAGGCTCTCATGACTCGAATACGATCAATTAAAGAAGAGATTGATAGAGTAAACCAAGAGATGGAAGCTGCTGAACGTGCCTATGACCTAAGTCGTGCTGCTGAGCTCAAATATGGAACTCTAATGTCCCTGCAACGCCAGTTAGAAGAGGCGGAGAAAAACCTTTCTGAGTACCAGAAATCTGGAAAATCTTTCCTACGAGAAGAAGTCACCGATCTTGATATTGCTGAGATTGTAAGCAAATGGACTGGTATACCCTTGTCAAACCTTCAACAGTCGGAAAGAGACAAGCTAGTCATGCTAGAACAGGTACTTCATAAGAGGGTAGTGGGTCAAGATATAGCCGTGAAGTCGGTAGCTGATGCAATCAGACGTTCAAGGGCTGGTCTATCAGACCCAAACCGACCTATTGCAAGTTTCATGTTTTTGGGACCTACTGGTGTGGGGAAAACAGAGCTTGCAAAGGCCTTGGCCAGCTATCTCTTCAACACAGAAAATGCTCTGGTTAGGATTGATATGAGTGAGTACATGGAAAAGCATGCAGTTTCACGTTTGGTGGGTGCTCCGCCTGGTTATGTTGGTTATGAAGAAGGTGGACAACTAACAGAAGTGGTTCGTCGAAGACCCTATTGTGTAGTGCTATTCGATGAAATAGAGAAAGCACATCAGGACGTGTTCAATATTTTGTTACAATTGCTGGATGATGGAAGGATAACTGACTCACAAGGAAGGACTGTAAGCTTCACCAATTGTGTTGTGATAATGACCTCAAATCTTGGTTCCCACTATATACTTGAAACTCTTCGTAATACTCAAGACAGCAAGGATGCAGTATATGAGTTGATGAAAAGACAAGTGGTTGAATTGGCGAGACAGACTTTCCGCCCTGAGTTTTTGAATCGCGTTGATGAGTTTATCGTGTTCCAGCCTTTGGACTCCAAAGAGATCTGTAACATTGTTGAGATACAGATGAACCGGGTTAAAGATAGGCTGAAGCAAAAGAAAATCGAGCTTCATTATACAAAGGAAGCTATGGAGCTTCTTGGTAAATTGGGCTTTGATCCGAACTTTGGAGCAAGGCCAGTAAAGAGGGTGATACAGCAGCTGGTTGAGAATGAAATTGCAATGGGACTGTTGAGAGGCGATTTCAGTGAGGAGGATGCGATCATTGTTGATGCTGAGGTGTCCCCATCCGCCAAAGATATTCCTCCACAGAAGAGGTTGCGCATCAGGAAATTGGAGAATGCTTCTTCTAGTGTAGATGACATGGTTGCGAATGACTGA

Protein Analysis

982

Amino Acids

109.71

Weight (kDa)

6.49

Isoelectric Point (pI)

37.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clp_N PF02861 95 - 218 3.6e-29 Clp repeat (R) N-terminal domain
NBD_SMAX1 PF23569 275 - 375 1.3e-06 SMAX1 nucleotide binding domain
AAA PF00004 294 - 425 8.2e-13 ATPase family associated with various cellular activities (AAA)
AAA_lid_9 PF17871 432 - 534 5.9e-35 AAA lid domain
AAA_2 PF07724 690 - 859 6.8e-69 AAA domain (Cdc48 subfamily)
AAA_5 PF07728 695 - 816 3.5e-12 AAA domain (dynein-related subfamily)
AAA PF00004 696 - 818 3.1e-10 ATPase family associated with various cellular activities (AAA)
ClpB_D2-small PF10431 866 - 946 4e-23 C-terminal, D2-small domain, of ClpB protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0009882)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G25140
fragaria_vesca FvH4_2g40970 FvH4_2g40970 FvH4_2g40970 FvH4_2g40970
malus_domestica MD08G1058100.v1.1 MD15G1038200.v1.1
prunus_persica Prupe.1G392200_v2.0.a1
pyrus_communis pycom08g04820 pycom15g03540
rosa_chinensis RchiOBHm_Chr6g0299601
rosa_laevigata RLG00000011406
rosa_multiflora Rmu_sc0002895.1_g000001
rosa_roxburghii Rroxscaffold_7G00168520
rosa_rugosa Rorug06G0290800 Rorug06G0290900
rosa_samantha Rh6AG401700 Rh6BG410100 Rh6CG415700 Rh6DG402900
rosa_wichuraiana Rw6G035150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1958
Acc16I TGCGCA 1 cut(s) 2891
Acc36I ACCTGC 1 cut(s) 1264
AccB1I GGYRCC 1 cut(s) 62
AccB7I CCANNNNNTGG 4 cut(s) 812, 1178, 1726, 2596
AccBSI CCGCTC 1 cut(s) 1336
AccI GTMKAC 3 cut(s) 435, 1515, 1918
AccII CGCG 1 cut(s) 2568
AccIII TCCGGA 1 cut(s) 955
AclWI GGATC 6 cut(s) 177, 797, 1241, 1254, 1665, 2720
AcoI YGGCCR 5 cut(s) 51, 189, 405, 634, 2136
AcsI RAATTY 1 cut(s) 1640
AcuI CTGAAG 4 cut(s) 753, 939, 1550, 2678
AcyI GRCGYC 1 cut(s) 63
AdeI CACNNNGTG 1 cut(s) 604
AfaI GTAC 6 cut(s) 1226, 1340, 1527, 1838, 1977, 2192
AfeI AGCGCT 1 cut(s) 262
AflIII ACRYGT 2 cut(s) 694, 2337
AjiI CACGTC 1 cut(s) 2338
AjnI CCWGG 5 cut(s) 384, 407, 854, 892, 2233
AjuI GAANNNNNNNTTGG 2 cut(s) 386, 418
AloI GAACNNNNNNTCC 2 cut(s) 50, 82
Alw21I GWGCWC 5 cut(s) 369, 600, 783, 1773, 2230
Alw26I GTCTC 2 cut(s) 1944, 2530
AlwI GGATC 6 cut(s) 177, 797, 1241, 1254, 1665, 2720
AlwNI CAGNNNCTG 1 cut(s) 2770
Aor13HI TCCGGA 1 cut(s) 955
Aor51HI AGCGCT 1 cut(s) 262
AoxI GGCC 8 cut(s) 51, 189, 405, 456, 634, 2130, 2136, 2744
ApoI RAATTY 1 cut(s) 1640
ArsI GACNNNNNNTTYG 2 cut(s) 614, 646
Asp700I GAANNNNTTC 3 cut(s) 284, 2277, 2466
AspLEI GCGC 4 cut(s) 65, 263, 536, 2892
AspS9I GGNCC 3 cut(s) 236, 509, 2096
AsuC2I CCSGG 1 cut(s) 2642
AsuHPI GGTGA 8 cut(s) 412, 502, 709, 1040, 1199, 1867, 2401, 2770
AsuNHI GCTAGC 1 cut(s) 5
AvaII GGWCC 3 cut(s) 236, 509, 2096
BaeGI GKGCMC 1 cut(s) 1378
BalI TGGCCA 3 cut(s) 407, 636, 2138
BamHI GGATCC 1 cut(s) 1246
BanI GGYRCC 1 cut(s) 62
BanII GRGCYC 2 cut(s) 783, 1773
BbsI GAAGAC 2 cut(s) 1308, 2293
Bbv12I GWGCWC 5 cut(s) 369, 600, 783, 1773, 2230
BbvCI CCTCAGC 1 cut(s) 2844
BccI CCATC 9 cut(s) 34, 289, 392, 487, 1300, 1534, 1720, 2363, 2863
BceAI ACGGC 3 cut(s) 76, 819, 1994
BcgI CGANNNNNNTGC 2 cut(s) 511, 545
BciT130I CCWGG 5 cut(s) 386, 409, 856, 894, 2235
BciVI GTATCC 1 cut(s) 851
BcnI CCSGG 1 cut(s) 2642
BcoDI GTCTC 2 cut(s) 1944, 2530
BfaI CTAG 4 cut(s) 6, 1958, 1967, 2919
BfmI CTRYAG 2 cut(s) 452, 1386
BfoI RGCGCY 3 cut(s) 66, 264, 537
BfuAI ACCTGC 1 cut(s) 1264
BfuI GTATCC 1 cut(s) 851
BglII AGATCT 1 cut(s) 2610
BlpI GCTNAGC 4 cut(s) 26, 273, 477, 1767
Bme1390I CCNGG 6 cut(s) 386, 409, 856, 894, 2235, 2642
Bme18I GGWCC 3 cut(s) 236, 509, 2096
BmgBI CACGTC 1 cut(s) 2338
BmgT120I GGNCC 3 cut(s) 236, 509, 2096
BmiI GGNNCC 9 cut(s) 64, 142, 150, 237, 960, 1015, 1248, 2097, 2446
BmrFI CCNGG 6 cut(s) 386, 409, 856, 894, 2235, 2642
BmsI GCATC 7 cut(s) 1141, 1357, 2017, 2482, 2815, 2830, 2901
BmtI GCTAGC 1 cut(s) 9
BoxI GACNNNNGTC 1 cut(s) 1755
BpiI GAAGAC 2 cut(s) 1308, 2293
BpmI CTGGAG 5 cut(s) 407, 641, 1032, 1277, 1622
Bpu10I CCTNAGC 2 cut(s) 267, 2844
Bpu1102I GCTNAGC 4 cut(s) 26, 273, 477, 1767
BpuEI CTTGAG 2 cut(s) 1682, 2463
BpuMI CCSGG 1 cut(s) 2642
BsaBI GATNNNNATC 2 cut(s) 174, 726
BsaHI GRCGYC 1 cut(s) 63
BsaJI CCNNGG 5 cut(s) 132, 144, 423, 486, 2133
BsaWI WCCGGW 1 cut(s) 955
BsaXI ACNNNNNCTCC 4 cut(s) 50, 80, 1250, 1280
Bse118I RCCGGY 1 cut(s) 9
Bse1I ACTGG 8 cut(s) 504, 786, 806, 1260, 1804, 1918, 2107, 2747
Bse3DI GCAATG 4 cut(s) 471, 1152, 1616, 2796
Bse8I GATNNNNATC 2 cut(s) 174, 726
BseAI TCCGGA 1 cut(s) 955
BseBI CCWGG 5 cut(s) 386, 409, 856, 894, 2235
BseDI CCNNGG 5 cut(s) 132, 144, 423, 486, 2133
BseGI GGATG 9 cut(s) 403, 826, 955, 1156, 1487, 2371, 2497, 2830, 2855
BseJI GATNNNNATC 2 cut(s) 174, 726
BseMI GCAATG 4 cut(s) 471, 1152, 1616, 2796
BseNI ACTGG 8 cut(s) 504, 786, 806, 1260, 1804, 1918, 2107, 2747
BseRI GAGGAG 1 cut(s) 2834
BseSI GKGCMC 1 cut(s) 1378
BseYI CCCAGC 1 cut(s) 1154
BsgI GTGCAG 1 cut(s) 1236
Bsh1236I CGCG 1 cut(s) 2568
BshFI GGCC 8 cut(s) 53, 191, 407, 458, 636, 2132, 2138, 2746
BshNI GGYRCC 1 cut(s) 62
BsiHKAI GWGCWC 5 cut(s) 369, 600, 783, 1773, 2230
BsiSI CCGG 3 cut(s) 10, 956, 2641
BslFI GGGAC 4 cut(s) 1777, 2109, 2808, 2836
BsmAI GTCTC 2 cut(s) 1944, 2530
BsmFI GGGAC 4 cut(s) 1777, 2109, 2808, 2836
BsmI GAATGC 2 cut(s) 553, 2914
BsnI GGCC 8 cut(s) 53, 191, 407, 458, 636, 2132, 2138, 2746
Bsp1286I GDGCHC 6 cut(s) 369, 600, 783, 1378, 1773, 2230
Bsp13I TCCGGA 1 cut(s) 955
Bsp1720I GCTNAGC 4 cut(s) 26, 273, 477, 1767
Bsp19I CCATGG 1 cut(s) 132
BspANI GGCC 8 cut(s) 53, 191, 407, 458, 636, 2132, 2138, 2746
BspEI TCCGGA 1 cut(s) 955
BspFNI CGCG 1 cut(s) 2568
BspHI TCATGA 1 cut(s) 1674
BspLI GGNNCC 9 cut(s) 64, 142, 150, 237, 960, 1015, 1248, 2097, 2446
BspMAI CTGCAG 2 cut(s) 456, 1390
BspMI ACCTGC 1 cut(s) 1264
BspOI GCTAGC 1 cut(s) 9
BspPI GGATC 6 cut(s) 177, 797, 1241, 1254, 1665, 2720
BspT107I GGYRCC 1 cut(s) 62
BsrBI CCGCTC 1 cut(s) 1336
BsrDI GCAATG 4 cut(s) 471, 1152, 1616, 2796
BsrFI RCCGGY 1 cut(s) 9
BsrI ACTGG 8 cut(s) 504, 786, 806, 1260, 1804, 1918, 2107, 2747
BssAI RCCGGY 1 cut(s) 9
BssECI CCNNGG 5 cut(s) 132, 144, 423, 486, 2133
BssNAI GTATAC 1 cut(s) 1919
BssNI GRCGYC 1 cut(s) 63
BssT1I CCWWGG 4 cut(s) 132, 423, 486, 2133
Bst1107I GTATAC 1 cut(s) 1919
Bst2UI CCWGG 5 cut(s) 386, 409, 856, 894, 2235
Bst4CI ACNGT 9 cut(s) 448, 630, 852, 1117, 1197, 1653, 1941, 2401, 2799
Bst6I CTCTTC 6 cut(s) 235, 1695, 1806, 2153, 2472, 2876
BstACI GRCGYC 1 cut(s) 63
BstAPI GCANNNNNTGC 1 cut(s) 1151
BstDSI CCRYGG 2 cut(s) 132, 144
BstENI CCTNNNNNAGG 1 cut(s) 516
BstF5I GGATG 9 cut(s) 403, 826, 955, 1156, 1487, 2371, 2497, 2830, 2855
BstFNI CGCG 1 cut(s) 2568
BstH2I RGCGCY 3 cut(s) 66, 264, 537
BstHHI GCGC 4 cut(s) 65, 263, 536, 2892
BstMAI GTCTC 2 cut(s) 1944, 2530
BstMWI GCNNNNNNNGC 9 cut(s) 116, 260, 269, 326, 1151, 1342, 1555, 1742, 1963
BstNI CCWGG 5 cut(s) 386, 409, 856, 894, 2235
BstNSI RCATGY 2 cut(s) 698, 2207
BstPAI GACNNNNGTC 1 cut(s) 1755
BstSCI CCNGG 6 cut(s) 384, 407, 854, 892, 2233, 2640
BstSFI CTRYAG 2 cut(s) 452, 1386
BstSLI GKGCMC 1 cut(s) 1378
BstUI CGCG 1 cut(s) 2568
BstV2I GAAGAC 2 cut(s) 1308, 2293
BstX2I RGATCY 3 cut(s) 1246, 1657, 2610
BstXI CCANNNNNNTGG 3 cut(s) 199, 493, 1847
BstYI RGATCY 3 cut(s) 1246, 1657, 2610
BstZ17I GTATAC 1 cut(s) 1919
BsuI GTATCC 1 cut(s) 851
BsuRI GGCC 8 cut(s) 53, 191, 407, 458, 636, 2132, 2138, 2746
BtgI CCRYGG 2 cut(s) 132, 144
BtgZI GCGATG 1 cut(s) 53
BtrI CACGTC 1 cut(s) 2338
BtsCI GGATG 9 cut(s) 403, 826, 955, 1156, 1487, 2371, 2497, 2830, 2855
BtsIMutI CAGTG 6 cut(s) 288, 444, 511, 1253, 1658, 2821
BveI ACCTGC 1 cut(s) 1264
CaiI CAGNNNCTG 1 cut(s) 2770
CciI TCATGA 1 cut(s) 1674
CfoI GCGC 4 cut(s) 65, 263, 536, 2892
Cfr10I RCCGGY 1 cut(s) 9
Cfr13I GGNCC 3 cut(s) 236, 509, 2096
Csp6I GTAC 6 cut(s) 1225, 1339, 1526, 1837, 1976, 2191
CspCI CAANNNNNGTGG 2 cut(s) 1006, 1041
CviQI GTAC 6 cut(s) 1225, 1339, 1526, 1837, 1976, 2191
DinI GGCGCC 1 cut(s) 64
DraIII CACNNNGTG 1 cut(s) 604
DrdI GACNNNNNNGTC 1 cut(s) 1958
DseDI GACNNNNNNGTC 1 cut(s) 1958
EaeI YGGCCR 5 cut(s) 51, 189, 405, 634, 2136
Eam1104I CTCTTC 6 cut(s) 235, 1695, 1806, 2153, 2472, 2876
EarI CTCTTC 6 cut(s) 235, 1695, 1806, 2153, 2472, 2876
EciI GGCGGA 5 cut(s) 241, 1832, 2220, 2537, 2848
Ecl136II GAGCTC 2 cut(s) 781, 1771
Eco130I CCWWGG 4 cut(s) 132, 423, 486, 2133
Eco147I AGGCCT 1 cut(s) 2132
Eco24I GRGCYC 2 cut(s) 783, 1773
Eco32I GATATC 1 cut(s) 1311
Eco47I GGWCC 3 cut(s) 236, 509, 2096
Eco47III AGCGCT 1 cut(s) 262
Eco53kI GAGCTC 2 cut(s) 781, 1771
Eco57I CTGAAG 4 cut(s) 753, 939, 1550, 2678
EcoICRI GAGCTC 2 cut(s) 781, 1771
EcoNI CCTNNNNNAGG 1 cut(s) 516
EcoO109I RGGNCCY 2 cut(s) 236, 2096
EcoRII CCWGG 5 cut(s) 384, 407, 854, 892, 2233
EcoRV GATATC 1 cut(s) 1311
EcoT14I CCWWGG 4 cut(s) 132, 423, 486, 2133
EcoT38I GRGCYC 2 cut(s) 783, 1773
EgeI GGCGCC 1 cut(s) 64
EheI GGCGCC 1 cut(s) 64
ErhI CCWWGG 4 cut(s) 132, 423, 486, 2133
FaqI GGGAC 4 cut(s) 1777, 2109, 2808, 2836
FauI CCCGC 1 cut(s) 1176
FblI GTMKAC 3 cut(s) 435, 1515, 1918
FokI GGATG 9 cut(s) 410, 833, 962, 1163, 1474, 2378, 2504, 2837, 2842
FriOI GRGCYC 2 cut(s) 783, 1773
FspBI CTAG 4 cut(s) 6, 1958, 1967, 2919
FspI TGCGCA 1 cut(s) 2891
GlaI GCGC 4 cut(s) 64, 262, 535, 2891
GsaI CCCAGC 1 cut(s) 1158
GsuI CTGGAG 5 cut(s) 407, 641, 1032, 1277, 1622
HaeII RGCGCY 3 cut(s) 66, 264, 537
HaeIII GGCC 8 cut(s) 53, 191, 407, 458, 636, 2132, 2138, 2746
HapII CCGG 3 cut(s) 10, 956, 2641
HhaI GCGC 4 cut(s) 65, 263, 536, 2892
Hin1I GRCGYC 1 cut(s) 63
Hin6I GCGC 4 cut(s) 63, 261, 534, 2890
HinP1I GCGC 4 cut(s) 63, 261, 534, 2890
HindIII AAGCTT 1 cut(s) 2404
HinfI GANTC 8 cut(s) 359, 414, 714, 1108, 1678, 2384, 2563, 2600
HpaII CCGG 3 cut(s) 10, 956, 2641
HphI GGTGA 8 cut(s) 412, 502, 709, 1040, 1199, 1867, 2401, 2770
Hpy166II GTNNAC 8 cut(s) 288, 436, 509, 1190, 1516, 1717, 1919, 2261
Hpy8I GTNNAC 8 cut(s) 288, 436, 509, 1190, 1516, 1717, 1919, 2261
Hpy99I CGWCG 2 cut(s) 22, 2286
HpyCH4III ACNGT 9 cut(s) 448, 630, 852, 1117, 1197, 1653, 1941, 2401, 2799
HpyCH4IV ACGT 5 cut(s) 1214, 1741, 2038, 2215, 2337
HpyF10VI GCNNNNNNNGC 9 cut(s) 116, 260, 269, 326, 1151, 1342, 1555, 1742, 1963
HpySE526I ACGT 5 cut(s) 1214, 1741, 2038, 2215, 2337
Hsp92I GRCGYC 1 cut(s) 63
HspAI GCGC 4 cut(s) 63, 261, 534, 2890
KasI GGCGCC 1 cut(s) 62
Kpn2I TCCGGA 1 cut(s) 955
KroI GCCGGC 1 cut(s) 9
KroNI GCCGGC 1 cut(s) 11
LweI GCATC 7 cut(s) 1141, 1357, 2017, 2482, 2815, 2830, 2901
MaeI CTAG 4 cut(s) 6, 1958, 1967, 2919
MaeII ACGT 5 cut(s) 1214, 1741, 2038, 2215, 2337
MaeIII GTNAC 7 cut(s) 715, 1028, 1066, 1607, 1873, 2352, 2615
MbiI CCGCTC 1 cut(s) 1336
MfeI CAATTG 3 cut(s) 641, 2357, 2413
MflI RGATCY 3 cut(s) 1246, 1657, 2610
MhlI GDGCHC 6 cut(s) 369, 600, 783, 1378, 1773, 2230
MlsI TGGCCA 3 cut(s) 407, 636, 2138
MluNI TGGCCA 3 cut(s) 407, 636, 2138
Mly113I GGCGCC 1 cut(s) 63
MlyI GAGTC 4 cut(s) 723, 1672, 2378, 2594
MmeI TCCRAC 6 cut(s) 197, 522, 645, 880, 1515, 1923
Mox20I TGGCCA 3 cut(s) 407, 636, 2138
MroI TCCGGA 1 cut(s) 955
MroNI GCCGGC 1 cut(s) 9
MroXI GAANNNNTTC 3 cut(s) 284, 2277, 2466
MscI TGGCCA 3 cut(s) 407, 636, 2138
MseI TTAA 5 cut(s) 690, 1592, 1644, 1695, 2646
MslI CAYNNNNRTG 3 cut(s) 293, 699, 1353
Msp20I TGGCCA 3 cut(s) 407, 636, 2138
MspA1I CMGCKG 1 cut(s) 2770
MspI CCGG 3 cut(s) 10, 956, 2641
MspR9I CCNGG 6 cut(s) 386, 409, 856, 894, 2235, 2642
MunI CAATTG 3 cut(s) 641, 2357, 2413
Mva1269I GAATGC 2 cut(s) 553, 2914
MvaI CCWGG 5 cut(s) 386, 409, 856, 894, 2235
MvnI CGCG 1 cut(s) 2568
MwoI GCNNNNNNNGC 9 cut(s) 116, 260, 269, 326, 1151, 1342, 1555, 1742, 1963
NaeI GCCGGC 1 cut(s) 11
NarI GGCGCC 1 cut(s) 63
NciI CCSGG 1 cut(s) 2642
NcoI CCATGG 1 cut(s) 132
NgoMIV GCCGGC 1 cut(s) 9
NheI GCTAGC 1 cut(s) 5
NlaIV GGNNCC 9 cut(s) 64, 142, 150, 237, 960, 1015, 1248, 2097, 2446
NmeAIII GCCGAG 1 cut(s) 510
NmuCI GTSAC 4 cut(s) 715, 1028, 1607, 1873
NsbI TGCGCA 1 cut(s) 2891
NspI RCATGY 2 cut(s) 698, 2207
PaeI GCATGC 1 cut(s) 2207
PagI TCATGA 1 cut(s) 1674
PceI AGGCCT 1 cut(s) 2132
PciI ACATGT 1 cut(s) 694
PcsI WCGNNNNNNNCGW 2 cut(s) 940, 1327
PctI GAATGC 2 cut(s) 553, 2914
PdiI GCCGGC 1 cut(s) 11
PdmI GAANNNNTTC 3 cut(s) 284, 2277, 2466
PfeI GAWTC 4 cut(s) 359, 414, 1108, 2563
PflMI CCANNNNNTGG 4 cut(s) 812, 1178, 1726, 2596
PfoI TCCNGGA 2 cut(s) 384, 854
PleI GAGTC 4 cut(s) 722, 1672, 2378, 2594
PluTI GGCGCC 1 cut(s) 66
PpsI GAGTC 4 cut(s) 722, 1672, 2378, 2594
PpuMI RGGWCCY 2 cut(s) 236, 2096
PscI ACATGT 1 cut(s) 694
PshAI GACNNNNGTC 1 cut(s) 1755
Psp124BI GAGCTC 2 cut(s) 783, 1773
Psp5II RGGWCCY 2 cut(s) 236, 2096
Psp6I CCWGG 5 cut(s) 384, 407, 854, 892, 2233
PspFI CCCAGC 1 cut(s) 1154
PspGI CCWGG 5 cut(s) 384, 407, 854, 892, 2233
PspN4I GGNNCC 9 cut(s) 64, 142, 150, 237, 960, 1015, 1248, 2097, 2446
PspPI GGNCC 3 cut(s) 236, 509, 2096
PspPPI RGGWCCY 2 cut(s) 236, 2096
PstI CTGCAG 2 cut(s) 456, 1390
PstNI CAGNNNCTG 1 cut(s) 2770
PsuI RGATCY 3 cut(s) 1246, 1657, 2610
PvuII CAGCTG 1 cut(s) 2770
RsaI GTAC 6 cut(s) 1226, 1340, 1527, 1838, 1977, 2192
RsaNI GTAC 6 cut(s) 1225, 1339, 1526, 1837, 1976, 2191
RseI CAYNNNNRTG 3 cut(s) 293, 699, 1353
SacI GAGCTC 2 cut(s) 783, 1773
SaqAI TTAA 5 cut(s) 690, 1592, 1644, 1695, 2646
Sau96I GGNCC 3 cut(s) 236, 509, 2096
SchI GAGTC 4 cut(s) 723, 1672, 2378, 2594
ScrFI CCNGG 6 cut(s) 386, 409, 856, 894, 2235, 2642
SduI GDGCHC 6 cut(s) 369, 600, 783, 1378, 1773, 2230
SfaNI GCATC 7 cut(s) 1141, 1357, 2017, 2482, 2815, 2830, 2901
SfcI CTRYAG 2 cut(s) 452, 1386
SfoI GGCGCC 1 cut(s) 64
SinI GGWCC 3 cut(s) 236, 509, 2096
SmiMI CAYNNNNRTG 3 cut(s) 293, 699, 1353
SmlI CTYRAG 2 cut(s) 1661, 2478
SmoI CTYRAG 2 cut(s) 1661, 2478
SphI GCATGC 1 cut(s) 2207
SseBI AGGCCT 1 cut(s) 2132
SspDI GGCGCC 1 cut(s) 62
SspI AATATT 1 cut(s) 2347
SspMI CTAG 4 cut(s) 6, 1958, 1967, 2919
SstI GAGCTC 2 cut(s) 783, 1773
StuI AGGCCT 1 cut(s) 2132
StyD4I CCNGG 6 cut(s) 384, 407, 854, 892, 2233, 2640
StyI CCWWGG 4 cut(s) 132, 423, 486, 2133
TaaI ACNGT 9 cut(s) 448, 630, 852, 1117, 1197, 1653, 1941, 2401, 2799
TaiI ACGT 5 cut(s) 1217, 1744, 2041, 2218, 2340
TaqI TCGA 7 cut(s) 172, 1299, 1580, 1681, 2284, 2310, 2673
TatI WGTACW 3 cut(s) 1224, 1525, 2190
TauI GCSGC 3 cut(s) 53, 141, 332
TfiI GAWTC 4 cut(s) 359, 414, 1108, 2563
Tru1I TTAA 5 cut(s) 690, 1592, 1644, 1695, 2646
Tru9I TTAA 5 cut(s) 690, 1592, 1644, 1695, 2646
TscAI CASTG 6 cut(s) 295, 451, 511, 1260, 1658, 2821
TseFI GTSAC 4 cut(s) 715, 1028, 1607, 1873
Tsp45I GTSAC 4 cut(s) 715, 1028, 1607, 1873
TspGWI ACGGA 2 cut(s) 133, 949
TspRI CASTG 6 cut(s) 295, 451, 511, 1260, 1658, 2821
Van91I CCANNNNNTGG 4 cut(s) 812, 1178, 1726, 2596
VpaK11BI GGWCC 3 cut(s) 236, 509, 2096
XagI CCTNNNNNAGG 1 cut(s) 516
XapI RAATTY 1 cut(s) 1640
XceI RCATGY 2 cut(s) 698, 2207
XcmI CCANNNNNNNNNTGG 1 cut(s) 130
XmiI GTMKAC 3 cut(s) 435, 1515, 1918
XmnI GAANNNNTTC 3 cut(s) 284, 2277, 2466
XspI CTAG 4 cut(s) 6, 1958, 1967, 2919
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.