RLG00000011406

Belongs to the ClpA ClpB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0009882
Physical Location & Seq
Forward (+)
9166327 .. 9170586
4260 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011406

Sequence Viewer

Length: 2940 bp
ATGGCTAGCCGGCAAGCGACGAAGCTCAGCAAATCGGCGATGGTTTTGGCGGCCATGAACGGCGCCTCAAGAAATTCCCTCTCTCGGTGTCGTGAAACTGCAGTGGCTGCCTCCAAGTTTTCCATGGGCGGCTCCGTGGCTCCCTTCAATGTCGTTTGGGATCGAAATCATGTCGTTTCGGCCAAGTATGTGGCAAACACTCTCACTCGGAGCTTTCACTCTTCGGGTCCTCGTTTCAGCTCCGCCAGCAGCGCTGCTCAGGCTCAGCAGAATGAGTTCACTGAGATGGCATGGGAGGGCATAATTGGGGCTGTGGAAGCCGCCAGAAATAGCAAGCAACAAGTGGTGGAATCTGAGCACTTGATGAAAGCTATCTTGGAGCAGAAGGATGGTTTGGCCAGGAGAATCTTCACCAAGGCTGGTGTAGACAACACCACTGTTCTCCAGGCCACCGATGACTTCATTGCTCAGCAACCCAAGGTGATGGGTGCGACCAGTGGACCTATAATAGGCACACATCTCGGCGCTCTGTTGGAGAATGCCCGATGGCAGAAGAAAGAAATGAATGATGATTTTGTGTCTGTGGAGCACCTTGTGTTGGCGTTTCAGTCCGACACACGGTTTGGGCAGCAATTGTTCAAAAACCTCCAGCTTAGTGACAAGGATTTGAAGGAGGCTGTTAAACATGTTCGTGGAAATCAGAGAGTCACTGATCAAAATCCTGAAGGGAAGTATGAGGCTTTGACTAAATATGGGAACGACTTGACTGAGCTCGCCAGTCGTGGCAAACTTGATCCAGTTATTGGACGGGATGATGAAATACGGCGTTGTATCCAAATACTGTCCAGGAGAACAAAAAATAATCCTGTTATTATTGGAGAGCCTGGTGTCGGAAAAACTGCAATCGCTGAAGGATTAGCTCAACGGATTGTGCGTGGGGATGTTCCGGAACCCCTATTGAACAGAAAGTTGATTTCTTTAGATATGGGTTCATTGGTCGCTGGAGCCAAGTTTCGCGGTGACTTTGAGGAAAGATTGAAAGCTGTATTGAAGGAAGTTACTGCTTCAAATGGACAGATCATATTATTCATTGATGAGATTCATACTGTTGTAGGAGCTGGGGCTACAAGTGGTGCAATGGATGCTGGTAACTTGTTGAAACCAATGCTTGGGCGGGGTGAACTACGGTGTATAGGAGCAACCACTTTGAATGAGTACAGAAAATACATTGAGAAGGATCCTGCACTGGAGCGTAGGTTTCAGCAGGTTTTTTGTGGTCAGCCATCTGTCGAAGACACAATATCCATTCTTCGAGGGTTGCGTGAGCGGTACGAGCTGCATCATGGTGTGAAAATATCAGATAGTGCCCTTGTTTCTGCAGCAGTTCTTTCTGACAGATACATTACGGAACGATTTTTGCCTGACAAAGCCATTGATCTTGTTGATGAAGCTGCTGCAAAGCTGAAAATGGAGATCACATCCAAGCCAACTGAGTTAGATGAGGTAGACAGAGCTGTACTGAAGTTGGAGATGGAGAAGCTATCTTTGCAAAATGACACTGATAAATCATCGAAAGAAAGGTTAAGCAAGCTGGAGAGTGACCTCGCATTGCTCAAACAGAAACAAAAAGAATTTAATGAACAGTGGGATCGTGAGATGGCTCTCATGACTCGAATACGATCAATTAAAGAAGAGATTGATAGAGTAAACCAAGAGATGGAAGCTGCTGAACGTGCATATGACCTAAGTCGTGCTGCCGAGCTCAAATATGGAACTCTAATGTCCCTGCAACGCCAGTTAGAAGAAGCTGAGAAAAACCTTTCTGAGTACCAGAAGTCTGGAAAATCTTTCCTACGAGAAGAAGTCACCGATCTTGATATTGCTGAGATTGTAAGCAAATGGACTGGTATACCTTTGTCAAACCTTCAACAGTCGGAAAGAGACAAGCTCGTCATGCTAGAACAGGTACTTCATAAGAGGGTAGTGGGTCAAGATATAGCGGTGAAATCGGTGGCTGATGCAATCAGACGTTCAAGGGCTGGTCTATCAGACCCAAACCGACCTATTGCAAGTTTCATGTTTTTGGGACCTACTGGTGTGGGGAAAACAGAGCTTGCAAAGGCCTTGGCCAGCTATCTCTTCAACACAGAAAATGCTCTGGTTAGGATTGATATGAGTGAGTACATGGAAAAGCATGCAGTTTCACGTTTGGTGGGTGCTCCACCTGGTTATGTTGGTTATGAAGAAGGTGGACAACTAACAGAAGTGGTTCGTCGAAGACCCTATTGTGTAGTGCTATTCGATGAAATAGAGAAAGCACATCAGGATGTGTTCAATATTTTGTTACAATTGCTGGATGATGGAAGGATAACCGACTCACAAGGAAGGACTGTAAGCTTCACCAATTGTGTTGTGATAATGACCTCAAATCTTGGCTCCCACTATATACTTGAAACTCTTCGTAATACTCAAGACAGCAAGGATGCAGTTTATGAGTTGATGAAAAGACAAGTGGTTGAATTGGCGAGACAGACTTTCCGCCCTGAGTTTTTGAATCGAGTTGATGAGTTTATCGTGTTCCAGCCTTTGGACTCCAAAGAGATCTGTAACATTGTTGACATACAGATGAACCGGGTTAAAGATAGGCTGAAGCAAAAGAAAATTGAGCTTCATTATACAAAGGAAGCTGTGGAGCTTCTTGGTAAATTGGGCTTTGATCCGAACTTTGGAGCAAGGCCAGTAAAGAGGGTGATACAGCAGCTGGTTGAGAATGAAATTGCAATGGGACTGTTGAGAGGCGATTTCAGTGATGAGGATGCGATCATTGTTGATGCTGAGGTGTCCCCATCCGCCAAAGATATTCCTCCACAGAAGAGGTTGCGCATCAGGAAATTGGAGAATGCTTCTTCTAGTGTAGATGACATGGTTGCGAATGACTGA

Protein Analysis

980

Amino Acids

109.46

Weight (kDa)

6.38

Isoelectric Point (pI)

36.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clp_N PF02861 92 - 215 2.4e-28 Clp repeat (R) N-terminal domain
NBD_SMAX1 PF23569 272 - 372 1.3e-06 SMAX1 nucleotide binding domain
AAA PF00004 291 - 422 8.1e-13 ATPase family associated with various cellular activities (AAA)
AAA_lid_9 PF17871 429 - 531 5.8e-35 AAA lid domain
AAA_2 PF07724 687 - 856 6.8e-69 AAA domain (Cdc48 subfamily)
AAA_5 PF07728 692 - 813 3.5e-12 AAA domain (dynein-related subfamily)
AAA PF00004 693 - 815 3.1e-10 ATPase family associated with various cellular activities (AAA)
ClpB_D2-small PF10431 863 - 943 1.7e-23 C-terminal, D2-small domain, of ClpB protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0009882)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G25140
fragaria_vesca FvH4_2g40970 FvH4_2g40970 FvH4_2g40970 FvH4_2g40970
malus_domestica MD08G1058100.v1.1 MD15G1038200.v1.1
prunus_persica Prupe.1G392200_v2.0.a1
pyrus_communis pycom08g04820 pycom15g03540
rosa_chinensis RchiOBHm_Chr6g0299601
rosa_laevigata RLG00000011406
rosa_multiflora Rmu_sc0002895.1_g000001
rosa_roxburghii Rroxscaffold_7G00168520
rosa_rugosa Rorug06G0290800 Rorug06G0290900
rosa_samantha Rh6AG401700 Rh6BG410100 Rh6CG415700 Rh6DG402900
rosa_wichuraiana Rw6G035150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1949
Acc16I TGCGCA 1 cut(s) 2882
Acc36I ACCTGC 1 cut(s) 1255
AccB1I GGYRCC 1 cut(s) 62
AccB7I CCANNNNNTGG 4 cut(s) 803, 1169, 1717, 2587
AccBSI CCGCTC 1 cut(s) 1327
AccI GTMKAC 3 cut(s) 426, 1506, 1909
AccII CGCG 1 cut(s) 1017
AccIII TCCGGA 1 cut(s) 946
AclWI GGATC 6 cut(s) 168, 788, 1232, 1245, 1656, 2711
AcoI YGGCCR 4 cut(s) 51, 180, 396, 2127
AcsI RAATTY 2 cut(s) 73, 1631
AcuI CTGAAG 4 cut(s) 744, 930, 1541, 2669
AcyI GRCGYC 1 cut(s) 63
AdeI CACNNNGTG 1 cut(s) 595
AfaI GTAC 6 cut(s) 1217, 1331, 1518, 1829, 1968, 2183
AfeI AGCGCT 1 cut(s) 253
AflIII ACRYGT 1 cut(s) 685
AjnI CCWGG 5 cut(s) 398, 444, 845, 883, 2224
AjuI GAANNNNNNNTTGG 4 cut(s) 359, 377, 391, 409
Alw21I GWGCWC 5 cut(s) 360, 591, 774, 1764, 2221
Alw26I GTCTC 2 cut(s) 1935, 2521
AlwI GGATC 6 cut(s) 168, 788, 1232, 1245, 1656, 2711
AlwNI CAGNNNCTG 2 cut(s) 107, 2761
Aor13HI TCCGGA 1 cut(s) 946
Aor51HI AGCGCT 1 cut(s) 253
AoxI GGCC 7 cut(s) 51, 180, 396, 447, 2121, 2127, 2735
ApoI RAATTY 2 cut(s) 73, 1631
ArsI GACNNNNNNTTYG 2 cut(s) 605, 637
Asp700I GAANNNNTTC 3 cut(s) 275, 2268, 2457
AspLEI GCGC 4 cut(s) 65, 254, 527, 2883
AspS9I GGNCC 3 cut(s) 227, 500, 2087
AsuC2I CCSGG 1 cut(s) 2633
AsuHPI GGTGA 8 cut(s) 403, 493, 1031, 1190, 1858, 2014, 2392, 2761
AsuNHI GCTAGC 1 cut(s) 5
AvaII GGWCC 3 cut(s) 227, 500, 2087
BaeGI GKGCMC 1 cut(s) 1369
BalI TGGCCA 2 cut(s) 398, 2129
BamHI GGATCC 1 cut(s) 1237
BanI GGYRCC 1 cut(s) 62
BanII GRGCYC 2 cut(s) 774, 1764
BbsI GAAGAC 2 cut(s) 1299, 2284
Bbv12I GWGCWC 5 cut(s) 360, 591, 774, 1764, 2221
BbvCI CCTCAGC 1 cut(s) 2835
BceAI ACGGC 2 cut(s) 76, 839
BcgI CGANNNNNNTGC 2 cut(s) 502, 536
BciT130I CCWGG 5 cut(s) 400, 446, 847, 885, 2226
BciVI GTATCC 1 cut(s) 842
BclI TGATCA 1 cut(s) 712
BcnI CCSGG 1 cut(s) 2633
BcoDI GTCTC 2 cut(s) 1935, 2521
BfaI CTAG 3 cut(s) 6, 1958, 2910
BfmI CTRYAG 2 cut(s) 99, 1377
BfoI RGCGCY 3 cut(s) 66, 255, 528
BfuAI ACCTGC 1 cut(s) 1255
BfuI GTATCC 1 cut(s) 842
BglII AGATCT 1 cut(s) 2601
BlpI GCTNAGC 3 cut(s) 26, 264, 468
Bme1390I CCNGG 6 cut(s) 400, 446, 847, 885, 2226, 2633
Bme18I GGWCC 3 cut(s) 227, 500, 2087
BmgT120I GGNCC 3 cut(s) 227, 500, 2087
BmiI GGNNCC 9 cut(s) 64, 133, 141, 228, 951, 1006, 1239, 2088, 2437
BmrFI CCNGG 6 cut(s) 400, 446, 847, 885, 2226, 2633
BmsI GCATC 7 cut(s) 1132, 1348, 2008, 2473, 2806, 2821, 2892
BmtI GCTAGC 1 cut(s) 9
BoxI GACNNNNGTC 1 cut(s) 1746
BpiI GAAGAC 2 cut(s) 1299, 2284
BplI GAGNNNNNCTC 2 cut(s) 1932, 1964
BpmI CTGGAG 5 cut(s) 428, 632, 1023, 1268, 1613
Bpu10I CCTNAGC 2 cut(s) 258, 2835
Bpu1102I GCTNAGC 3 cut(s) 26, 264, 468
BpuEI CTTGAG 2 cut(s) 52, 2454
BpuMI CCSGG 1 cut(s) 2633
BsaBI GATNNNNATC 2 cut(s) 165, 717
BsaHI GRCGYC 1 cut(s) 63
BsaJI CCNNGG 5 cut(s) 123, 135, 414, 477, 2124
BsaWI WCCGGW 1 cut(s) 946
BsaXI ACNNNNNCTCC 2 cut(s) 1241, 1271
Bse118I RCCGGY 1 cut(s) 9
Bse1I ACTGG 8 cut(s) 495, 777, 797, 1251, 1795, 1909, 2098, 2738
Bse3DI GCAATG 4 cut(s) 462, 1143, 1607, 2787
Bse8I GATNNNNATC 2 cut(s) 165, 717
BseAI TCCGGA 1 cut(s) 946
BseBI CCWGG 5 cut(s) 400, 446, 847, 885, 2226
BseDI CCNNGG 5 cut(s) 123, 135, 414, 477, 2124
BseJI GATNNNNATC 2 cut(s) 165, 717
BseMI GCAATG 4 cut(s) 462, 1143, 1607, 2787
BseNI ACTGG 8 cut(s) 495, 777, 797, 1251, 1795, 1909, 2098, 2738
BseSI GKGCMC 1 cut(s) 1369
BseYI CCCAGC 1 cut(s) 1118
BsgI GTGCAG 1 cut(s) 1227
Bsh1236I CGCG 1 cut(s) 1017
BshFI GGCC 7 cut(s) 53, 182, 398, 449, 2123, 2129, 2737
BshNI GGYRCC 1 cut(s) 62
BsiHKAI GWGCWC 5 cut(s) 360, 591, 774, 1764, 2221
BsiSI CCGG 3 cut(s) 10, 947, 2632
BslFI GGGAC 4 cut(s) 1768, 2100, 2799, 2827
BsmAI GTCTC 2 cut(s) 1935, 2521
BsmFI GGGAC 4 cut(s) 1768, 2100, 2799, 2827
BsmI GAATGC 2 cut(s) 544, 2905
BsnI GGCC 7 cut(s) 53, 182, 398, 449, 2123, 2129, 2737
Bsp1286I GDGCHC 6 cut(s) 360, 591, 774, 1369, 1764, 2221
Bsp13I TCCGGA 1 cut(s) 946
Bsp1720I GCTNAGC 3 cut(s) 26, 264, 468
Bsp19I CCATGG 1 cut(s) 123
BspANI GGCC 7 cut(s) 53, 182, 398, 449, 2123, 2129, 2737
BspEI TCCGGA 1 cut(s) 946
BspFNI CGCG 1 cut(s) 1017
BspHI TCATGA 1 cut(s) 1665
BspLI GGNNCC 9 cut(s) 64, 133, 141, 228, 951, 1006, 1239, 2088, 2437
BspMAI CTGCAG 2 cut(s) 103, 1381
BspMI ACCTGC 1 cut(s) 1255
BspOI GCTAGC 1 cut(s) 9
BspPI GGATC 6 cut(s) 168, 788, 1232, 1245, 1656, 2711
BspT107I GGYRCC 1 cut(s) 62
BsrBI CCGCTC 1 cut(s) 1327
BsrDI GCAATG 4 cut(s) 462, 1143, 1607, 2787
BsrFI RCCGGY 1 cut(s) 9
BsrI ACTGG 8 cut(s) 495, 777, 797, 1251, 1795, 1909, 2098, 2738
BssAI RCCGGY 1 cut(s) 9
BssECI CCNNGG 5 cut(s) 123, 135, 414, 477, 2124
BssNAI GTATAC 1 cut(s) 1910
BssNI GRCGYC 1 cut(s) 63
BssT1I CCWWGG 4 cut(s) 123, 414, 477, 2124
Bst1107I GTATAC 1 cut(s) 1910
Bst2UI CCWGG 5 cut(s) 400, 446, 847, 885, 2226
Bst4CI ACNGT 9 cut(s) 439, 621, 843, 1108, 1188, 1644, 1932, 2392, 2790
Bst6I CTCTTC 5 cut(s) 226, 1686, 2144, 2463, 2867
BstACI GRCGYC 1 cut(s) 63
BstAPI GCANNNNNTGC 2 cut(s) 107, 1142
BstDSI CCRYGG 2 cut(s) 123, 135
BstENI CCTNNNNNAGG 1 cut(s) 507
BstFNI CGCG 1 cut(s) 1017
BstH2I RGCGCY 3 cut(s) 66, 255, 528
BstHHI GCGC 4 cut(s) 65, 254, 527, 2883
BstMAI GTCTC 2 cut(s) 1935, 2521
BstNI CCWGG 5 cut(s) 400, 446, 847, 885, 2226
BstNSI RCATGY 2 cut(s) 689, 2198
BstPAI GACNNNNGTC 1 cut(s) 1746
BstSCI CCNGG 6 cut(s) 398, 444, 845, 883, 2224, 2631
BstSFI CTRYAG 2 cut(s) 99, 1377
BstSLI GKGCMC 1 cut(s) 1369
BstUI CGCG 1 cut(s) 1017
BstV2I GAAGAC 2 cut(s) 1299, 2284
BstX2I RGATCY 2 cut(s) 1237, 2601
BstXI CCANNNNNNTGG 3 cut(s) 190, 484, 1838
BstYI RGATCY 2 cut(s) 1237, 2601
BstZ17I GTATAC 1 cut(s) 1910
BsuI GTATCC 1 cut(s) 842
BsuRI GGCC 7 cut(s) 53, 182, 398, 449, 2123, 2129, 2737
BtgI CCRYGG 2 cut(s) 123, 135
BtgZI GCGATG 1 cut(s) 53
BtsI GCAGTG 1 cut(s) 108
BtsIMutI CAGTG 9 cut(s) 108, 279, 435, 502, 708, 1244, 1557, 1649, 2812
BveI ACCTGC 1 cut(s) 1255
CaiI CAGNNNCTG 2 cut(s) 107, 2761
CciI TCATGA 1 cut(s) 1665
CfoI GCGC 4 cut(s) 65, 254, 527, 2883
Cfr10I RCCGGY 1 cut(s) 9
Cfr13I GGNCC 3 cut(s) 227, 500, 2087
CsiI ACCWGGT 1 cut(s) 2224
Csp6I GTAC 6 cut(s) 1216, 1330, 1517, 1828, 1967, 2182
CviQI GTAC 6 cut(s) 1216, 1330, 1517, 1828, 1967, 2182
DinI GGCGCC 1 cut(s) 64
DraIII CACNNNGTG 1 cut(s) 595
DrdI GACNNNNNNGTC 1 cut(s) 1949
DseDI GACNNNNNNGTC 1 cut(s) 1949
EaeI YGGCCR 4 cut(s) 51, 180, 396, 2127
Eam1104I CTCTTC 5 cut(s) 226, 1686, 2144, 2463, 2867
EarI CTCTTC 5 cut(s) 226, 1686, 2144, 2463, 2867
EciI GGCGGA 3 cut(s) 232, 2528, 2839
Ecl136II GAGCTC 2 cut(s) 772, 1762
Eco130I CCWWGG 4 cut(s) 123, 414, 477, 2124
Eco147I AGGCCT 1 cut(s) 2123
Eco24I GRGCYC 2 cut(s) 774, 1764
Eco47I GGWCC 3 cut(s) 227, 500, 2087
Eco47III AGCGCT 1 cut(s) 253
Eco53kI GAGCTC 2 cut(s) 772, 1762
Eco57I CTGAAG 4 cut(s) 744, 930, 1541, 2669
EcoICRI GAGCTC 2 cut(s) 772, 1762
EcoNI CCTNNNNNAGG 1 cut(s) 507
EcoO109I RGGNCCY 2 cut(s) 227, 2087
EcoRII CCWGG 5 cut(s) 398, 444, 845, 883, 2224
EcoT14I CCWWGG 4 cut(s) 123, 414, 477, 2124
EcoT38I GRGCYC 2 cut(s) 774, 1764
EgeI GGCGCC 1 cut(s) 64
EheI GGCGCC 1 cut(s) 64
ErhI CCWWGG 4 cut(s) 123, 414, 477, 2124
FaqI GGGAC 4 cut(s) 1768, 2100, 2799, 2827
FauI CCCGC 1 cut(s) 1167
FauNDI CATATG 1 cut(s) 1738
FbaI TGATCA 1 cut(s) 712
FblI GTMKAC 3 cut(s) 426, 1506, 1909
FriOI GRGCYC 2 cut(s) 774, 1764
FspBI CTAG 3 cut(s) 6, 1958, 2910
FspI TGCGCA 1 cut(s) 2882
GlaI GCGC 4 cut(s) 64, 253, 526, 2882
GsaI CCCAGC 1 cut(s) 1122
GsuI CTGGAG 5 cut(s) 428, 632, 1023, 1268, 1613
HaeII RGCGCY 3 cut(s) 66, 255, 528
HaeIII GGCC 7 cut(s) 53, 182, 398, 449, 2123, 2129, 2737
HapII CCGG 3 cut(s) 10, 947, 2632
HhaI GCGC 4 cut(s) 65, 254, 527, 2883
Hin1I GRCGYC 1 cut(s) 63
Hin6I GCGC 4 cut(s) 63, 252, 525, 2881
HinP1I GCGC 4 cut(s) 63, 252, 525, 2881
HincII GTYRAC 1 cut(s) 2617
HindII GTYRAC 1 cut(s) 2617
HindIII AAGCTT 1 cut(s) 2395
HinfI GANTC 8 cut(s) 350, 405, 705, 1099, 1669, 2375, 2554, 2591
HpaII CCGG 3 cut(s) 10, 947, 2632
HphI GGTGA 8 cut(s) 403, 493, 1031, 1190, 1858, 2014, 2392, 2761
Hpy166II GTNNAC 9 cut(s) 279, 427, 500, 1181, 1507, 1708, 1910, 2252, 2617
Hpy8I GTNNAC 9 cut(s) 279, 427, 500, 1181, 1507, 1708, 1910, 2252, 2617
Hpy99I CGWCG 2 cut(s) 22, 2277
HpyCH4III ACNGT 9 cut(s) 439, 621, 843, 1108, 1188, 1644, 1932, 2392, 2790
HpyCH4IV ACGT 3 cut(s) 1732, 2029, 2206
HpySE526I ACGT 3 cut(s) 1732, 2029, 2206
Hsp92I GRCGYC 1 cut(s) 63
HspAI GCGC 4 cut(s) 63, 252, 525, 2881
KasI GGCGCC 1 cut(s) 62
Kpn2I TCCGGA 1 cut(s) 946
KroI GCCGGC 1 cut(s) 9
KroNI GCCGGC 1 cut(s) 11
Ksp22I TGATCA 1 cut(s) 712
LweI GCATC 7 cut(s) 1132, 1348, 2008, 2473, 2806, 2821, 2892
MabI ACCWGGT 1 cut(s) 2224
MaeI CTAG 3 cut(s) 6, 1958, 2910
MaeII ACGT 3 cut(s) 1732, 2029, 2206
MaeIII GTNAC 9 cut(s) 656, 706, 1019, 1057, 1148, 1598, 1864, 2343, 2606
MbiI CCGCTC 1 cut(s) 1327
MfeI CAATTG 3 cut(s) 632, 2348, 2404
MflI RGATCY 2 cut(s) 1237, 2601
MhlI GDGCHC 6 cut(s) 360, 591, 774, 1369, 1764, 2221
MlsI TGGCCA 2 cut(s) 398, 2129
MluNI TGGCCA 2 cut(s) 398, 2129
Mly113I GGCGCC 1 cut(s) 63
MlyI GAGTC 4 cut(s) 714, 1663, 2369, 2585
MmeI TCCRAC 5 cut(s) 513, 636, 871, 1506, 1914
Mox20I TGGCCA 2 cut(s) 398, 2129
MroI TCCGGA 1 cut(s) 946
MroNI GCCGGC 1 cut(s) 9
MroXI GAANNNNTTC 3 cut(s) 275, 2268, 2457
MscI TGGCCA 2 cut(s) 398, 2129
MseI TTAA 5 cut(s) 681, 1583, 1635, 1686, 2637
MslI CAYNNNNRTG 5 cut(s) 284, 690, 1344, 2325, 2624
Msp20I TGGCCA 2 cut(s) 398, 2129
MspA1I CMGCKG 1 cut(s) 2761
MspI CCGG 3 cut(s) 10, 947, 2632
MspR9I CCNGG 6 cut(s) 400, 446, 847, 885, 2226, 2633
MunI CAATTG 3 cut(s) 632, 2348, 2404
Mva1269I GAATGC 2 cut(s) 544, 2905
MvaI CCWGG 5 cut(s) 400, 446, 847, 885, 2226
MvnI CGCG 1 cut(s) 1017
NaeI GCCGGC 1 cut(s) 11
NarI GGCGCC 1 cut(s) 63
NciI CCSGG 1 cut(s) 2633
NcoI CCATGG 1 cut(s) 123
NdeI CATATG 1 cut(s) 1738
NgoMIV GCCGGC 1 cut(s) 9
NheI GCTAGC 1 cut(s) 5
NlaIV GGNNCC 9 cut(s) 64, 133, 141, 228, 951, 1006, 1239, 2088, 2437
NmeAIII GCCGAG 2 cut(s) 501, 1783
NmuCI GTSAC 5 cut(s) 656, 706, 1019, 1598, 1864
NsbI TGCGCA 1 cut(s) 2882
NspI RCATGY 2 cut(s) 689, 2198
PaeI GCATGC 1 cut(s) 2198
PagI TCATGA 1 cut(s) 1665
PceI AGGCCT 1 cut(s) 2123
PciI ACATGT 1 cut(s) 685
PcsI WCGNNNNNNNCGW 2 cut(s) 931, 1318
PctI GAATGC 2 cut(s) 544, 2905
PdiI GCCGGC 1 cut(s) 11
PdmI GAANNNNTTC 3 cut(s) 275, 2268, 2457
PfeI GAWTC 4 cut(s) 350, 405, 1099, 2554
PflMI CCANNNNNTGG 4 cut(s) 803, 1169, 1717, 2587
PfoI TCCNGGA 1 cut(s) 845
PleI GAGTC 4 cut(s) 713, 1663, 2369, 2585
PluTI GGCGCC 1 cut(s) 66
PpsI GAGTC 4 cut(s) 713, 1663, 2369, 2585
PpuMI RGGWCCY 2 cut(s) 227, 2087
PscI ACATGT 1 cut(s) 685
PshAI GACNNNNGTC 1 cut(s) 1746
Psp124BI GAGCTC 2 cut(s) 774, 1764
Psp5II RGGWCCY 2 cut(s) 227, 2087
Psp6I CCWGG 5 cut(s) 398, 444, 845, 883, 2224
PspFI CCCAGC 1 cut(s) 1118
PspGI CCWGG 5 cut(s) 398, 444, 845, 883, 2224
PspN4I GGNNCC 9 cut(s) 64, 133, 141, 228, 951, 1006, 1239, 2088, 2437
PspPI GGNCC 3 cut(s) 227, 500, 2087
PspPPI RGGWCCY 2 cut(s) 227, 2087
PstI CTGCAG 2 cut(s) 103, 1381
PstNI CAGNNNCTG 2 cut(s) 107, 2761
PsuI RGATCY 2 cut(s) 1237, 2601
PvuII CAGCTG 1 cut(s) 2761
RsaI GTAC 6 cut(s) 1217, 1331, 1518, 1829, 1968, 2183
RsaNI GTAC 6 cut(s) 1216, 1330, 1517, 1828, 1967, 2182
RseI CAYNNNNRTG 5 cut(s) 284, 690, 1344, 2325, 2624
SacI GAGCTC 2 cut(s) 774, 1764
SaqAI TTAA 5 cut(s) 681, 1583, 1635, 1686, 2637
Sau96I GGNCC 3 cut(s) 227, 500, 2087
SchI GAGTC 4 cut(s) 714, 1663, 2369, 2585
ScrFI CCNGG 6 cut(s) 400, 446, 847, 885, 2226, 2633
SduI GDGCHC 6 cut(s) 360, 591, 774, 1369, 1764, 2221
SexAI ACCWGGT 1 cut(s) 2224
SfaNI GCATC 7 cut(s) 1132, 1348, 2008, 2473, 2806, 2821, 2892
SfcI CTRYAG 2 cut(s) 99, 1377
SfoI GGCGCC 1 cut(s) 64
SinI GGWCC 3 cut(s) 227, 500, 2087
SmiMI CAYNNNNRTG 5 cut(s) 284, 690, 1344, 2325, 2624
SmlI CTYRAG 2 cut(s) 67, 2469
SmoI CTYRAG 2 cut(s) 67, 2469
SphI GCATGC 1 cut(s) 2198
SseBI AGGCCT 1 cut(s) 2123
SspDI GGCGCC 1 cut(s) 62
SspI AATATT 1 cut(s) 2338
SspMI CTAG 3 cut(s) 6, 1958, 2910
SstI GAGCTC 2 cut(s) 774, 1764
StuI AGGCCT 1 cut(s) 2123
StyD4I CCNGG 6 cut(s) 398, 444, 845, 883, 2224, 2631
StyI CCWWGG 4 cut(s) 123, 414, 477, 2124
TaaI ACNGT 9 cut(s) 439, 621, 843, 1108, 1188, 1644, 1932, 2392, 2790
TaiI ACGT 3 cut(s) 1735, 2032, 2209
TaqI TCGA 8 cut(s) 163, 1290, 1312, 1571, 1672, 2275, 2301, 2557
TatI WGTACW 3 cut(s) 1215, 1516, 2181
TauI GCSGC 3 cut(s) 53, 132, 323
TfiI GAWTC 4 cut(s) 350, 405, 1099, 2554
Tru1I TTAA 5 cut(s) 681, 1583, 1635, 1686, 2637
Tru9I TTAA 5 cut(s) 681, 1583, 1635, 1686, 2637
TscAI CASTG 9 cut(s) 108, 286, 442, 502, 715, 1251, 1564, 1649, 2812
TseFI GTSAC 5 cut(s) 656, 706, 1019, 1598, 1864
Tsp45I GTSAC 5 cut(s) 656, 706, 1019, 1598, 1864
TspGWI ACGGA 3 cut(s) 124, 940, 1421
TspRI CASTG 9 cut(s) 108, 286, 442, 502, 715, 1251, 1564, 1649, 2812
Van91I CCANNNNNTGG 4 cut(s) 803, 1169, 1717, 2587
VpaK11BI GGWCC 3 cut(s) 227, 500, 2087
XagI CCTNNNNNAGG 1 cut(s) 507
XapI RAATTY 2 cut(s) 73, 1631
XceI RCATGY 2 cut(s) 689, 2198
XcmI CCANNNNNNNNNTGG 1 cut(s) 121
XmiI GTMKAC 3 cut(s) 426, 1506, 1909
XmnI GAANNNNTTC 3 cut(s) 275, 2268, 2457
XspI CTAG 3 cut(s) 6, 1958, 2910
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.