MD15G1038200.v1.1

Belongs to the ClpA ClpB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0009882
Physical Location & Seq
Forward (+)
2663551 .. 2667930
4380 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1038200.v1.1.491

Sequence Viewer

Length: 2901 bp
ATGGCGAGCAGGAGAGCCACCACGACACTCACCAAGTCGGCCTTAACTCTGGCGTCCCTGAACGCCACTAAATCCTCGAGAATCCCTCTCTCTCGGAGTCGCGCAATCGCCGTCGCCGCATCCGCCCGGACCTTCGGCAGCTCTGCCGTTCCCTTCTCTCGCCCCAGCTTTGTTCCCGAGGGCAGAAATGTCGCTTCGGCCAAGTATTTGGCAAGCGCCTTCACTCGCAGCTTCCACTCTACGACTCCCAGTTTCTCCTCAGCCACGAGCTCCTCTCAGGCCAATCAGAATGAGTTCACTGAGATGGCGTGGGAGGGCATTGTTGGTGCCGTTGACGCCGCCCGATATAGCAAGCAGCAGGTGGTGGAATCTGAGCACTTGATGAAAGCACTTTTGGAGCAGAAGGATGGTTTGGCCCGGAGAATTTTCACCAAGGCTGGGCTCGACAACACTACGGTTCTTCAAGCAACTGACGACTTCATAGATAAGCAACCCAAGGTGACGGGTGGCACCAGTGGCCCTATTATGGGTTCACATCTGGTTGGTTTGTTGGACAATGCTCGGCGGCAGAAGAAAGATATGAAAGATGATTTCGTTTCAGTGGAGCACCTTGTGTTGGCGTTTCAGGCCGATGCGCGGTTTGGGCAGCAATTGTTCAGAAACCTCCAGCTTAGTGATAAGGATTTGAAGGAGGCTGTTAAAGATGTTCGTGGCAATCAGAGAGCCACTGATCAAAATCCTGAAGGAAAATATGAGGCCTTGGATAAGTACGGGAATGATTTGACTGAACTCGCTAGGCGTGGCAAACTTGATCCTGTCATAGGCCGAGATGATGAAATACGGCGGTGTATCCAGATATTGTCTAGGAGAACGAAGAATAATCCTGTTATTATTGGTGAGCCTGGTGTGGGAAAAACAGCAATCGCTGAAGGATTAGCTCAACGCATTGTGCGTGGTGATGTTCCAGAACCGCTATTGAATAGAAAGTTGATTTCTTTAGATATGGGATCGTTGGTTGCTGGTGCCAAATTTCGTGGGGATTTTGAGGAAAGGTTGAAAGCTGTTCTGAAGGAAGAGCTGGTTTGGGCTACAAGTGGTGCGATGGATGCTGGGAACTTGTTGAAGCCAATGCTTGGGCGAGGTGAGCTTCGGTGTATAGGAGCAACTACATTGAATGAGTGCAGAAAATACATTGAGAAGGATGCTGCACTTGAGCGTAGATTTCAACAAGTATTTTGTGGTCAGCCATCTGTGGAAGACACAATTTCTATTCTTCGTGGCTTGCGTGAGCGCTATGAACTGCATCATGGTGTGAAAATATCAGATAGTGCTCTTGTTTCTGCAGCAGTTCTCTCAGACAGATACATTACAGAACGTTTTTTGCCTGACAAAGCCATTGATCTGGTTGATGAAGCTGCTGCAAAGCTGAAGATGGAGATCACATCTAAGCCAACCGAGTTGGATGAGGTAGACCGAGCTGTGTTGAAGTTGGAGATGGAGAAGCTATCAGTGCAAAATGACACTGATAAATCATCGAAAGAAAGGTTAAGCAAGTTGGAAAGTGACCTGGCAGTGCTGAAACAGAAACAGAAAGAACTAAGTGAACAATGGGATCGTGAAAAGGCTCTCATGACTCGCATACGATCAATTAAAGAAGAGATTGATAGAGTAAACCAAGAGATGGAAGCTGCTGAACGAGACTACAACCTAAATCGTGCTGCTGAGCTTAAGTATGGAACGCTAATGTCCCTACAACGCCAGTTAGAAGAGGCTGAGAACAACCTTGCCGAGTATCGGAAGTCTGGAAACTCTATGCTTCGTGAAGAAGTCACAGATCTTGATATTGCTGAAATTGTAAGCAAATGGACTGGCATACCCTTGTCAAACCTTCAACAATCAGAAAGAGACAAGCTCGTTATGCTGGAACAGGTTCTTCATAAGAGGGTGATTGGTCAAGATATAGCTGTGAAATCCGTAGCGGACGCAATTCGACGTTCAAGGGCAGGTCTATCTGACCCAAATCGACCAATAGCTAGTTTTATGTTTATGGGTCCTACTGGTGTGGGAAAAACCGAGCTTGCAAAGGCCTTGGCTAGCTGTCTCTTTAACACAGAAAATGCTCTTGTCAGAATTGATATGAGTGAGTACATGGAAAAACATGCGGTTTCACGCTTGGTTGGGGCACCACCTGGTTACGTCGGTTACGAAGAAGGTGGGCAGCTAACAGAAGTGGTTCGTCGAAGACCTTATTGTGTAGTGCTATTCGATGAAATTGAGAAAGCGCATCATGATGTCTTCAACATTTTGTTACAACTGTTGGATGATGGGAGGATAACCGACTCCCAAGGGCGAACTGTAAGCTTCACCAATTGTGTCGTGATAATGACCTCGAACCTGGGCTCCCACTACATACTCGAAACTCTTCGTAATGCACACGACAGCAAGGATGCAGTTTATCAAGTGATGAAAGGACAGGTCGTTGAGTTGGCAAGACAAGCTTTCCGCCCTGAATTTATGAACCGAATCGATGAGTATATTGTGTTCCAGCCTTTGGATTCGAAAGAGATTGGTAACATTGTCGAGATACAGATGAACCGACTGAAAGACAGGCTGAAGCAGAGGAAAATTGATCTTCATTACACGGAGGAAGCCGTGGAGCTTCTCGGGACACTAGGCTTCGATCCCAACTTTGGCGCAAGGCCGGTGAAGCGGGTGATACAACAGTTGGTTGAGAATGAAATTGCAATGGGAGTCCTGAGAGGAGATTTCAACGAGGAAGACTCGCTTATTGTCGATGCCGAGGCGTCCCCGTCCGCCAAAGACCTTCCTCCCCAGAAGAGGTTGCGGATCGAGAAATTGGAGAGCACTTCTGCTGCAGATGCCATGGTTGTGAACGACTGA

Protein Analysis

967

Amino Acids

107.83

Weight (kDa)

6.35

Isoelectric Point (pI)

40.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clp_N PF02861 98 - 218 8.4e-29 Clp repeat (R) N-terminal domain
AAA_lid_9 PF17871 417 - 519 1.2e-34 AAA lid domain
AAA_2 PF07724 675 - 844 2.7e-68 AAA domain (Cdc48 subfamily)
AAA_5 PF07728 680 - 801 9.6e-12 AAA domain (dynein-related subfamily)
AAA PF00004 681 - 803 1.4e-09 ATPase family associated with various cellular activities (AAA)
ClpB_D2-small PF10431 851 - 930 5.1e-23 C-terminal, D2-small domain, of ClpB protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0009882)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G25140
fragaria_vesca FvH4_2g40970 FvH4_2g40970 FvH4_2g40970 FvH4_2g40970
malus_domestica MD08G1058100.v1.1 MD15G1038200.v1.1
prunus_persica Prupe.1G392200_v2.0.a1
pyrus_communis pycom08g04820 pycom15g03540
rosa_chinensis RchiOBHm_Chr6g0299601
rosa_laevigata RLG00000011406
rosa_multiflora Rmu_sc0002895.1_g000001
rosa_roxburghii Rroxscaffold_7G00168520
rosa_rugosa Rorug06G0290800 Rorug06G0290900
rosa_samantha Rh6AG401700 Rh6BG410100 Rh6CG415700 Rh6DG402900
rosa_wichuraiana Rw6G035150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 349
Acc36I ACCTGC 2 cut(s) 349, 1994
AccB1I GGYRCC 4 cut(s) 326, 509, 1022, 2182
AccB7I CCANNNNNTGG 3 cut(s) 1133, 1681, 2551
AccI GTMKAC 1 cut(s) 1470
AccII CGCG 2 cut(s) 102, 637
AclI AACGTT 1 cut(s) 1375
AclWI GGATC 5 cut(s) 806, 1015, 1620, 2675, 2855
AcoI YGGCCR 1 cut(s) 198
AcsI RAATTY 3 cut(s) 423, 1028, 2510
AcuI CTGAAG 5 cut(s) 762, 948, 1088, 1448, 2633
AcyI GRCGYC 3 cut(s) 53, 336, 2804
AdeI CACNNNGTG 1 cut(s) 613
AfaI GTAC 2 cut(s) 770, 2147
AfeI AGCGCT 1 cut(s) 1292
AflII CTTAAG 1 cut(s) 1727
AjnI CCWGG 4 cut(s) 901, 1566, 2188, 2394
AjuI GAANNNNNNNTTGG 4 cut(s) 377, 395, 409, 427
AloI GAACNNNNNNTCC 2 cut(s) 2384, 2416
Alw21I GWGCWC 5 cut(s) 272, 378, 609, 1333, 2867
Alw26I GTCTC 3 cut(s) 1692, 1899, 2105
AlwI GGATC 5 cut(s) 806, 1015, 1620, 2675, 2855
Ama87I CYCGRG 3 cut(s) 76, 176, 2663
Aor51HI AGCGCT 1 cut(s) 1292
ApoI RAATTY 3 cut(s) 423, 1028, 2510
Asp700I GAANNNNTTC 4 cut(s) 293, 1929, 2232, 2421
AspLEI GCGC 6 cut(s) 104, 218, 637, 1293, 2284, 2696
AspS9I GGNCC 4 cut(s) 129, 415, 518, 2051
AsuC2I CCSGG 2 cut(s) 127, 418
AsuII TTCGAA 1 cut(s) 2558
AsuNHI GCTAGC 1 cut(s) 2093
AvaI CYCGRG 3 cut(s) 76, 176, 2663
AvaII GGWCC 2 cut(s) 129, 2051
BaeGI GKGCMC 1 cut(s) 2185
BaeI ACNNNNGTAYC 2 cut(s) 2708, 2741
BanI GGYRCC 4 cut(s) 326, 509, 1022, 2182
BanII GRGCYC 3 cut(s) 272, 444, 2402
BauI CACGAG 1 cut(s) 265
BbsI GAAGAC 4 cut(s) 1263, 2248, 2287, 2784
Bbv12I GWGCWC 5 cut(s) 272, 378, 609, 1333, 2867
BbvCI CCTCAGC 1 cut(s) 259
BccI CCATC 8 cut(s) 298, 401, 1096, 1255, 1426, 1489, 1675, 2318
BceAI ACGGC 5 cut(s) 95, 131, 314, 857, 2636
BcgI CGANNNNNNTGC 2 cut(s) 172, 206
BciT130I CCWGG 4 cut(s) 903, 1568, 2190, 2396
BciVI GTATCC 1 cut(s) 860
BclI TGATCA 1 cut(s) 730
BcnI CCSGG 2 cut(s) 127, 418
BcoDI GTCTC 3 cut(s) 1692, 1899, 2105
BfaI CTAG 5 cut(s) 795, 864, 2034, 2094, 2672
BfmI CTRYAG 2 cut(s) 1341, 2874
BfoI RGCGCY 2 cut(s) 219, 1294
BfrI CTTAAG 1 cut(s) 1727
BfuAI ACCTGC 2 cut(s) 349, 1994
BfuI GTATCC 1 cut(s) 860
BglII AGATCT 1 cut(s) 1834
BlpI GCTNAGC 1 cut(s) 1722
Bme1390I CCNGG 6 cut(s) 127, 418, 903, 1568, 2190, 2396
Bme18I GGWCC 2 cut(s) 129, 2051
BmeT110I CYCGRG 3 cut(s) 76, 176, 2663
BmgT120I GGNCC 4 cut(s) 129, 415, 518, 2051
BmiI GGNNCC 6 cut(s) 328, 511, 1024, 2052, 2184, 2401
BmrFI CCNGG 6 cut(s) 127, 418, 903, 1568, 2190, 2396
BmrI ACTGGG 1 cut(s) 243
BmsI GCATC 9 cut(s) 128, 622, 1096, 1192, 1312, 2293, 2437, 2785, 2869
BmtI GCTAGC 1 cut(s) 2097
BmuI ACTGGG 1 cut(s) 243
BpiI GAAGAC 4 cut(s) 1263, 2248, 2287, 2784
BplI GAGNNNNNCTC 8 cut(s) 70, 102, 259, 291, 1896, 1928, 2765, 2797
BpmI CTGGAG 1 cut(s) 650
Bpu10I CCTNAGC 1 cut(s) 259
Bpu1102I GCTNAGC 1 cut(s) 1722
Bpu14I TTCGAA 1 cut(s) 2558
BpuEI CTTGAG 1 cut(s) 1232
BpuMI CCSGG 2 cut(s) 127, 418
Bsa29I ATCGAT 1 cut(s) 2526
BsaBI GATNNNNATC 2 cut(s) 735, 1436
BsaHI GRCGYC 3 cut(s) 53, 336, 2804
BsaXI ACNNNNNCTCC 2 cut(s) 2384, 2414
Bse118I RCCGGY 1 cut(s) 2701
Bse1I ACTGG 5 cut(s) 249, 513, 1759, 1873, 2062
Bse3DI GCAATG 1 cut(s) 2751
Bse8I GATNNNNATC 2 cut(s) 735, 1436
BseBI CCWGG 4 cut(s) 903, 1568, 2190, 2396
BseCI ATCGAT 1 cut(s) 2526
BseGI GGATG 7 cut(s) 119, 412, 1111, 1207, 1468, 2326, 2452
BseJI GATNNNNATC 2 cut(s) 735, 1436
BseMI GCAATG 1 cut(s) 2751
BseMII CTCAG 8 cut(s) 273, 290, 291, 363, 1368, 1713, 1764, 2747
BseNI ACTGG 5 cut(s) 249, 513, 1759, 1873, 2062
BseRI GAGGAG 3 cut(s) 247, 262, 2775
BseSI GKGCMC 1 cut(s) 2185
BseYI CCCAGC 3 cut(s) 164, 437, 1109
BsgI GTGCAG 2 cut(s) 1191, 1201
Bsh1236I CGCG 2 cut(s) 102, 637
BshNI GGYRCC 4 cut(s) 326, 509, 1022, 2182
BshVI ATCGAT 1 cut(s) 2526
BsiHKAI GWGCWC 5 cut(s) 272, 378, 609, 1333, 2867
BsiHKCI CYCGRG 3 cut(s) 76, 176, 2663
BsiSI CCGG 3 cut(s) 127, 418, 2702
BslFI GGGAC 4 cut(s) 40, 1732, 2680, 2791
BsmAI GTCTC 3 cut(s) 1692, 1899, 2105
BsmFI GGGAC 4 cut(s) 40, 1732, 2680, 2791
BsoBI CYCGRG 3 cut(s) 76, 176, 2663
Bsp119I TTCGAA 1 cut(s) 2558
Bsp1286I GDGCHC 8 cut(s) 272, 378, 444, 609, 1333, 2185, 2402, 2867
Bsp1720I GCTNAGC 1 cut(s) 1722
Bsp19I CCATGG 1 cut(s) 2883
BspCNI CTCAG 8 cut(s) 272, 289, 292, 364, 1367, 1714, 1765, 2748
BspDI ATCGAT 1 cut(s) 2526
BspFNI CGCG 2 cut(s) 102, 637
BspHI TCATGA 2 cut(s) 1629, 2287
BspLI GGNNCC 6 cut(s) 328, 511, 1024, 2052, 2184, 2401
BspMAI CTGCAG 2 cut(s) 1345, 2878
BspMI ACCTGC 2 cut(s) 349, 1994
BspOI GCTAGC 1 cut(s) 2097
BspPI GGATC 5 cut(s) 806, 1015, 1620, 2675, 2855
BspQI GCTCTTC 1 cut(s) 1068
BspT104I TTCGAA 1 cut(s) 2558
BspT107I GGYRCC 4 cut(s) 326, 509, 1022, 2182
BspTI CTTAAG 1 cut(s) 1727
BsrDI GCAATG 1 cut(s) 2751
BsrFI RCCGGY 1 cut(s) 2701
BsrI ACTGG 5 cut(s) 249, 513, 1759, 1873, 2062
BssAI RCCGGY 1 cut(s) 2701
BssNI GRCGYC 3 cut(s) 53, 336, 2804
BssSI CACGAG 1 cut(s) 265
BssT1I CCWWGG 6 cut(s) 432, 495, 759, 2088, 2344, 2883
Bst2BI CACGAG 1 cut(s) 265
Bst2UI CCWGG 4 cut(s) 903, 1568, 2190, 2396
Bst4CI ACNGT 4 cut(s) 457, 2316, 2356, 2724
Bst6I CTCTTC 5 cut(s) 1068, 1650, 1761, 2427, 2831
BstACI GRCGYC 3 cut(s) 53, 336, 2804
BstAFI CTTAAG 1 cut(s) 1727
BstBI TTCGAA 1 cut(s) 2558
BstC8I GCNNGC 6 cut(s) 7, 214, 353, 1283, 2079, 2095
BstDSI CCRYGG 2 cut(s) 2652, 2883
BstENI CCTNNNNNAGG 1 cut(s) 819
BstF5I GGATG 7 cut(s) 119, 412, 1111, 1207, 1468, 2326, 2452
BstFNI CGCG 2 cut(s) 102, 637
BstH2I RGCGCY 2 cut(s) 219, 1294
BstHHI GCGC 6 cut(s) 104, 218, 637, 1293, 2284, 2696
BstMAI GTCTC 3 cut(s) 1692, 1899, 2105
BstNI CCWGG 4 cut(s) 903, 1568, 2190, 2396
BstNSI RCATGY 1 cut(s) 2162
BstSCI CCNGG 6 cut(s) 125, 416, 901, 1566, 2188, 2394
BstSFI CTRYAG 2 cut(s) 1341, 2874
BstSLI GKGCMC 1 cut(s) 2185
BstUI CGCG 2 cut(s) 102, 637
BstV2I GAAGAC 4 cut(s) 1263, 2248, 2287, 2784
BstX2I RGATCY 1 cut(s) 1834
BstXI CCANNNNNNTGG 2 cut(s) 208, 1402
BstYI RGATCY 1 cut(s) 1834
Bsu15I ATCGAT 1 cut(s) 2526
BsuI GTATCC 1 cut(s) 860
BsuTUI ATCGAT 1 cut(s) 2526
BtgI CCRYGG 2 cut(s) 2652, 2883
BtgZI GCGATG 1 cut(s) 1115
BtsCI GGATG 7 cut(s) 119, 412, 1111, 1207, 1468, 2326, 2452
BtsI GCAGTG 1 cut(s) 1578
BtsIMutI CAGTG 7 cut(s) 297, 520, 606, 726, 1515, 1521, 1578
BveI ACCTGC 2 cut(s) 349, 1994
Cac8I GCNNGC 6 cut(s) 7, 214, 353, 1283, 2079, 2095
CciI TCATGA 2 cut(s) 1629, 2287
CfoI GCGC 6 cut(s) 104, 218, 637, 1293, 2284, 2696
Cfr10I RCCGGY 1 cut(s) 2701
Cfr13I GGNCC 4 cut(s) 129, 415, 518, 2051
ClaI ATCGAT 1 cut(s) 2526
CseI GACGC 4 cut(s) 42, 344, 1991, 2793
CsiI ACCWGGT 1 cut(s) 2188
Csp6I GTAC 2 cut(s) 769, 2146
CspCI CAANNNNNGTGG 2 cut(s) 1015, 1050
CviAII CATG 6 cut(s) 1307, 1630, 2149, 2159, 2288, 2884
CviQI GTAC 2 cut(s) 769, 2146
DraIII CACNNNGTG 1 cut(s) 613
EaeI YGGCCR 1 cut(s) 198
Eam1104I CTCTTC 5 cut(s) 1068, 1650, 1761, 2427, 2831
EarI CTCTTC 5 cut(s) 1068, 1650, 1761, 2427, 2831
EciI GGCGGA 3 cut(s) 112, 2492, 2803
Ecl136II GAGCTC 1 cut(s) 270
Eco130I CCWWGG 6 cut(s) 432, 495, 759, 2088, 2344, 2883
Eco147I AGGCCT 2 cut(s) 758, 2087
Eco24I GRGCYC 3 cut(s) 272, 444, 2402
Eco47I GGWCC 2 cut(s) 129, 2051
Eco47III AGCGCT 1 cut(s) 1292
Eco53kI GAGCTC 1 cut(s) 270
Eco57I CTGAAG 5 cut(s) 762, 948, 1088, 1448, 2633
Eco88I CYCGRG 3 cut(s) 76, 176, 2663
EcoICRI GAGCTC 1 cut(s) 270
EcoNI CCTNNNNNAGG 1 cut(s) 819
EcoO109I RGGNCCY 1 cut(s) 2051
EcoRII CCWGG 4 cut(s) 901, 1566, 2188, 2394
EcoT14I CCWWGG 6 cut(s) 432, 495, 759, 2088, 2344, 2883
EcoT38I GRGCYC 3 cut(s) 272, 444, 2402
ErhI CCWWGG 6 cut(s) 432, 495, 759, 2088, 2344, 2883
FaeI CATG 6 cut(s) 1310, 1633, 2152, 2162, 2291, 2887
FalI AAGNNNNNCTT 6 cut(s) 26, 58, 2482, 2514, 2769, 2801
FaqI GGGAC 4 cut(s) 40, 1732, 2680, 2791
FatI CATG 6 cut(s) 1306, 1629, 2148, 2158, 2287, 2883
FauI CCCGC 1 cut(s) 2703
FbaI TGATCA 1 cut(s) 730
FblI GTMKAC 1 cut(s) 1470
FokI GGATG 7 cut(s) 106, 419, 1118, 1214, 1475, 2333, 2459
FriOI GRGCYC 3 cut(s) 272, 444, 2402
FspBI CTAG 5 cut(s) 795, 864, 2034, 2094, 2672
GlaI GCGC 6 cut(s) 103, 217, 636, 1292, 2283, 2695
GsaI CCCAGC 3 cut(s) 168, 441, 1113
GsuI CTGGAG 1 cut(s) 650
HaeII RGCGCY 2 cut(s) 219, 1294
HapII CCGG 3 cut(s) 127, 418, 2702
HgaI GACGC 4 cut(s) 42, 344, 1991, 2793
HhaI GCGC 6 cut(s) 104, 218, 637, 1293, 2284, 2696
Hin1I GRCGYC 3 cut(s) 53, 336, 2804
Hin1II CATG 6 cut(s) 1310, 1633, 2152, 2162, 2291, 2887
Hin6I GCGC 6 cut(s) 102, 216, 635, 1291, 2282, 2694
HinP1I GCGC 6 cut(s) 102, 216, 635, 1291, 2282, 2694
HincII GTYRAC 1 cut(s) 334
HindII GTYRAC 1 cut(s) 334
HindIII AAGCTT 2 cut(s) 2359, 2496
HpaII CCGG 3 cut(s) 127, 418, 2702
Hpy166II GTNNAC 7 cut(s) 297, 334, 533, 1471, 1604, 1672, 2893
Hpy8I GTNNAC 7 cut(s) 297, 334, 533, 1471, 1604, 1672, 2893
Hpy99I CGWCG 4 cut(s) 116, 1995, 2201, 2241
HpyCH4III ACNGT 4 cut(s) 457, 2316, 2356, 2724
HpyCH4IV ACGT 3 cut(s) 1375, 1993, 2196
HpySE526I ACGT 3 cut(s) 1375, 1993, 2196
Hsp92I GRCGYC 3 cut(s) 53, 336, 2804
Hsp92II CATG 6 cut(s) 1310, 1633, 2152, 2162, 2291, 2887
HspAI GCGC 6 cut(s) 102, 216, 635, 1291, 2282, 2694
Ksp22I TGATCA 1 cut(s) 730
LguI GCTCTTC 1 cut(s) 1068
LmnI GCTCC 6 cut(s) 275, 397, 604, 1160, 2405, 2656
LweI GCATC 9 cut(s) 128, 622, 1096, 1192, 1312, 2293, 2437, 2785, 2869
MabI ACCWGGT 1 cut(s) 2188
MaeI CTAG 5 cut(s) 795, 864, 2034, 2094, 2672
MaeII ACGT 3 cut(s) 1375, 1993, 2196
MaeIII GTNAC 7 cut(s) 499, 1562, 1828, 2192, 2201, 2307, 2570
MfeI CAATTG 2 cut(s) 650, 2368
MflI RGATCY 1 cut(s) 1834
MhlI GDGCHC 8 cut(s) 272, 378, 444, 609, 1333, 2185, 2402, 2867
MlyI GAGTC 6 cut(s) 106, 238, 1627, 2333, 2760, 2774
MmeI TCCRAC 5 cut(s) 531, 1440, 1470, 1536, 2298
MroXI GAANNNNTTC 4 cut(s) 293, 1929, 2232, 2421
MseI TTAA 6 cut(s) 44, 699, 1547, 1650, 1728, 2106
MslI CAYNNNNRTG 4 cut(s) 302, 1308, 2289, 2888
MspCI CTTAAG 1 cut(s) 1727
MspI CCGG 3 cut(s) 127, 418, 2702
MspR9I CCNGG 6 cut(s) 127, 418, 903, 1568, 2190, 2396
MunI CAATTG 2 cut(s) 650, 2368
MvaI CCWGG 4 cut(s) 903, 1568, 2190, 2396
MvnI CGCG 2 cut(s) 102, 637
NciI CCSGG 2 cut(s) 127, 418
NcoI CCATGG 1 cut(s) 2883
NheI GCTAGC 1 cut(s) 2093
NlaIII CATG 6 cut(s) 1310, 1633, 2152, 2162, 2291, 2887
NlaIV GGNNCC 6 cut(s) 328, 511, 1024, 2052, 2184, 2401
NmeAIII GCCGAG 4 cut(s) 541, 851, 1813, 2824
NmuCI GTSAC 3 cut(s) 499, 1562, 1828
NspI RCATGY 1 cut(s) 2162
NspV TTCGAA 1 cut(s) 2558
PaeR7I CTCGAG 1 cut(s) 76
PagI TCATGA 2 cut(s) 1629, 2287
PaqCI CACCTGC 1 cut(s) 349
PceI AGGCCT 2 cut(s) 758, 2087
PciSI GCTCTTC 1 cut(s) 1068
PcsI WCGNNNNNNNCGW 3 cut(s) 949, 1282, 2202
PdmI GAANNNNTTC 4 cut(s) 293, 1929, 2232, 2421
PfeI GAWTC 4 cut(s) 81, 368, 2523, 2555
PflMI CCANNNNNTGG 3 cut(s) 1133, 1681, 2551
PleI GAGTC 6 cut(s) 105, 238, 1627, 2333, 2759, 2774
PpsI GAGTC 6 cut(s) 105, 238, 1627, 2333, 2759, 2774
PpuMI RGGWCCY 1 cut(s) 2051
Psp124BI GAGCTC 1 cut(s) 272
Psp1406I AACGTT 1 cut(s) 1375
Psp5II RGGWCCY 1 cut(s) 2051
Psp6I CCWGG 4 cut(s) 901, 1566, 2188, 2394
PspFI CCCAGC 3 cut(s) 164, 437, 1109
PspGI CCWGG 4 cut(s) 901, 1566, 2188, 2394
PspN4I GGNNCC 6 cut(s) 328, 511, 1024, 2052, 2184, 2401
PspPI GGNCC 4 cut(s) 129, 415, 518, 2051
PspPPI RGGWCCY 1 cut(s) 2051
PsrI GAACNNNNNNTAC 2 cut(s) 2525, 2557
PstI CTGCAG 2 cut(s) 1345, 2878
PsuI RGATCY 1 cut(s) 1834
RsaI GTAC 2 cut(s) 770, 2147
RsaNI GTAC 2 cut(s) 769, 2146
RseI CAYNNNNRTG 4 cut(s) 302, 1308, 2289, 2888
SacI GAGCTC 1 cut(s) 272
SapI GCTCTTC 1 cut(s) 1068
SaqAI TTAA 6 cut(s) 44, 699, 1547, 1650, 1728, 2106
Sau96I GGNCC 4 cut(s) 129, 415, 518, 2051
SchI GAGTC 6 cut(s) 106, 238, 1627, 2333, 2760, 2774
ScrFI CCNGG 6 cut(s) 127, 418, 903, 1568, 2190, 2396
SduI GDGCHC 8 cut(s) 272, 378, 444, 609, 1333, 2185, 2402, 2867
SexAI ACCWGGT 1 cut(s) 2188
SfaNI GCATC 9 cut(s) 128, 622, 1096, 1192, 1312, 2293, 2437, 2785, 2869
SfcI CTRYAG 2 cut(s) 1341, 2874
Sfr274I CTCGAG 1 cut(s) 76
SfuI TTCGAA 1 cut(s) 2558
SinI GGWCC 2 cut(s) 129, 2051
SlaI CTCGAG 1 cut(s) 76
SmiMI CAYNNNNRTG 4 cut(s) 302, 1308, 2289, 2888
SmlI CTYRAG 3 cut(s) 76, 1211, 1727
SmoI CTYRAG 3 cut(s) 76, 1211, 1727
SseBI AGGCCT 2 cut(s) 758, 2087
SspMI CTAG 5 cut(s) 795, 864, 2034, 2094, 2672
SstI GAGCTC 1 cut(s) 272
StuI AGGCCT 2 cut(s) 758, 2087
StyD4I CCNGG 6 cut(s) 125, 416, 901, 1566, 2188, 2394
StyI CCWWGG 6 cut(s) 432, 495, 759, 2088, 2344, 2883
TaaI ACNGT 4 cut(s) 457, 2316, 2356, 2724
TaiI ACGT 3 cut(s) 1378, 1996, 2199
TaqII GACCGA 1 cut(s) 1488
TatI WGTACW 1 cut(s) 2145
TauI GCSGC 3 cut(s) 119, 341, 568
TfiI GAWTC 4 cut(s) 81, 368, 2523, 2555
Tru1I TTAA 6 cut(s) 44, 699, 1547, 1650, 1728, 2106
Tru9I TTAA 6 cut(s) 44, 699, 1547, 1650, 1728, 2106
TscAI CASTG 7 cut(s) 304, 520, 606, 733, 1515, 1528, 1578
TseFI GTSAC 3 cut(s) 499, 1562, 1828
Tsp45I GTSAC 3 cut(s) 499, 1562, 1828
TspGWI ACGGA 2 cut(s) 1963, 2657
TspRI CASTG 7 cut(s) 304, 520, 606, 733, 1515, 1528, 1578
Van91I CCANNNNNTGG 3 cut(s) 1133, 1681, 2551
Vha464I CTTAAG 1 cut(s) 1727
VpaK11BI GGWCC 2 cut(s) 129, 2051
XagI CCTNNNNNAGG 1 cut(s) 819
XapI RAATTY 3 cut(s) 423, 1028, 2510
XceI RCATGY 1 cut(s) 2162
XhoI CTCGAG 1 cut(s) 76
XmiI GTMKAC 1 cut(s) 1470
XmnI GAANNNNTTC 4 cut(s) 293, 1929, 2232, 2421
XspI CTAG 5 cut(s) 795, 864, 2034, 2094, 2672
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.