AT2G26060

Essential component of the cytosolic iron-sulfur (Fe S) protein assembly machinery. Required for the maturation of extramitochondrial Fe S proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0007416
Physical Location & Seq
Forward (+)
11102223 .. 11105364
3142 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G26060.2

Sequence Viewer

Length: 1059 bp
ATGGATTTGATGGAGAAGAACTTGGAGTTAGTGGAGATTCAGAAACTAGAAGGCCACACCGATCGTGTTTGGAGCGTAGCTTGGAACCCCGTTTCTTCTCACGCCGACGGTGTTTCACCGATTCTTGCTTCTTGCAGTGGCGATAACACTGTTCGAATCTGGGAACAAAGCTCTCTCTCTCGCTCCTGGACTTGCAAGACAGTTTTGGAAGAGACGCATACAAGAACTGTGAGGTCGTGCGCTTGGTCACCCTCAGGACAGTTATTGGCCACTGCAAGTTTTGATGGTACCACTGGCATTTGGAAGAATTACGGTTCTGAGTTTGAGTGTATTTCCACTTTGGAGGGACATGAAAACGAAGTCAAAAGTGTATCATGGAATGCATCTGGTTCATGCCTTGCAACATGTAGTAGAGATAAGTCTGTTTGGATTTGGGAAGTGCTTGAAGGGAATGAATATGACTGTGCTGCGGTATTAACTGGGCATACACAAGATGTGAAGATGGTTCAGTGGCATCCCACCATGGATGTTTTATTTTCTTGCAGTTATGATAACACCATCAAGGTTTGGTGGTCTGAAGATGATGATGGTGAGTATCAATGTGTCCAAACCTTAGGTGAATCTAACAACGGTCACTCTTCAACGGTATGGTCCATCTCATTTAACGCTGCAGGGGACAAGATGGTCACTTGTAGTGATGATCTAACCTTGAAGATATGGGGGACAGATATTGCCAAGATGCAGTCTGGTGAAGAATATGCACCTTGGATTCATCTTTGTACTCTCTCTGGCTATCATGACCGTACCATATACTCAGCTCACTGGTCAAGGGACGACATTATTGCCAGTGGAGCAGGCGATAATGCTATACGGTTGTTTGTGGACAGCAAACATGACTCTGTTGATGGACCTTCATATAATCTTTTGCTGAAGAAGAATAAAGCACATGAAAATGATGTAAACTCTGTCCAATGGTCACCCGGTGAGGGGAACCGGTTGCTTGCGTCGGCTAGTGATGATGGGATGGTCAAGATTTGGCAGCTTGCAACTAAACCGTGA

Protein Analysis

352

Amino Acids

39.13

Weight (kDa)

4.81

Isoelectric Point (pI)

34.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 10 - 67 5.3e-07 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 12 - 101 6.3e-09 CDC20/Fizzy WD40 domain
WD40 PF00400 13 - 54 4.3e-09 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 13 - 102 5.8e-14 WDR5 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 13 - 241 2e-24 WDR3 second beta-propeller domain
WD40_Gbeta PF25391 15 - 240 2.4e-10 G protein beta WD-40 repeat protein
Beta-prop_CAF1B_HIR1 PF24105 21 - 189 6e-14 CAF1B/HIR1 beta-propeller domain
Beta-prop_EML_2 PF23414 39 - 193 8.1e-17 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_TEP1_2nd PF25047 39 - 101 2.8e-06 TEP-1 second beta-propeller
WD40 PF00400 64 - 101 1.1e-07 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 68 - 240 1.1e-27 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 71 - 152 8.7e-13 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 71 - 193 8.5e-16 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 71 - 246 1.1e-34 WDR5 beta-propeller domain
EIF3I PF24805 71 - 152 1.8e-07 EIF3I
Beta-prop_THOC3 PF25174 73 - 126 7.5e-10 THOC3 beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 74 - 187 1.3e-07 MABP1/WDR62 second WD40 domain
WDR55 PF24796 76 - 150 9.2e-07 WDR55
Beta-prop_WDR19_1st PF23389 80 - 178 8e-06 WDR19 first beta-propeller
Beta-prop_TEP1_2nd PF25047 106 - 194 7.3e-09 TEP-1 second beta-propeller
WD40 PF00400 109 - 145 3.9e-10 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 117 - 194 2e-18 THOC3 beta-propeller domain
Beta-prop_Aladin PF25460 118 - 191 4.1e-07 Aladin seven-bladed propeller
Beta-prop_EIPR1 PF23609 131 - 240 3.4e-06 EIPR1 beta-propeller
WD40_Prp19 PF24814 154 - 241 4.6e-13 Prp19 WD40 domain
WD40 PF00400 155 - 190 1.5e-08 WD domain, G-beta repeat
EIF3I PF24805 158 - 270 1.2e-11 EIF3I
WDR55 PF24796 164 - 293 9.7e-06 WDR55
WD40 PF00400 201 - 240 4.6e-07 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 212 - 293 1e-07 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 683
Acc65I GGTACC 1 cut(s) 287
AccB1I GGYRCC 1 cut(s) 287
AciI CCGC 1 cut(s) 470
AcoI YGGCCR 1 cut(s) 267
AcuI CTGAAG 2 cut(s) 597, 950
AdeI CACNNNGTG 1 cut(s) 983
AfaI GTAC 3 cut(s) 289, 781, 805
AfiI CCNNNNNNNGG 2 cut(s) 986, 987
AflIII ACRYGT 1 cut(s) 404
AgeI ACCGGT 1 cut(s) 993
AgsI TTSAA 3 cut(s) 446, 642, 712
AjnI CCWGG 1 cut(s) 185
AluBI AGCT 4 cut(s) 80, 171, 818, 1042
AluI AGCT 4 cut(s) 80, 171, 818, 1042
Alw26I GTCTC 1 cut(s) 206
AoxI GGCC 2 cut(s) 52, 267
ApeKI GCWGC 3 cut(s) 467, 668, 1039
AsiGI ACCGGT 1 cut(s) 993
Asp718I GGTACC 1 cut(s) 287
AspLEI GCGC 1 cut(s) 242
AspS9I GGNCC 2 cut(s) 651, 908
AsuC2I CCSGG 1 cut(s) 981
AsuHPI GGTGA 7 cut(s) 108, 240, 602, 629, 761, 969, 995
AsuII TTCGAA 1 cut(s) 154
AvaII GGWCC 2 cut(s) 651, 908
AxyI CCTNAGG 2 cut(s) 253, 613
BalI TGGCCA 1 cut(s) 269
BanI GGYRCC 1 cut(s) 287
BbvI GCAGC 3 cut(s) 454, 655, 1051
BcgI CGANNNNNNTGC 2 cut(s) 824, 858
BciT130I CCWGG 1 cut(s) 187
BcnI CCSGG 1 cut(s) 981
BcoDI GTCTC 1 cut(s) 206
BfaI CTAG 2 cut(s) 47, 1011
BfmI CTRYAG 1 cut(s) 669
BisI GCNGC 3 cut(s) 468, 669, 1040
BlsI GCNGC 3 cut(s) 469, 670, 1041
Bme1390I CCNGG 2 cut(s) 187, 981
Bme18I GGWCC 2 cut(s) 651, 908
BmgT120I GGNCC 2 cut(s) 651, 908
BmiI GGNNCC 3 cut(s) 86, 289, 992
BmrFI CCNGG 2 cut(s) 187, 981
BmrI ACTGGG 1 cut(s) 489
BmsI GCATC 3 cut(s) 392, 523, 729
BmuI ACTGGG 1 cut(s) 489
Bpu14I TTCGAA 1 cut(s) 154
BpuMI CCSGG 1 cut(s) 981
BsaJI CCNNGG 2 cut(s) 522, 764
BsaWI WCCGGW 1 cut(s) 993
Bsc4I CCNNNNNNNGG 2 cut(s) 986, 987
Bse118I RCCGGY 1 cut(s) 993
Bse1I ACTGG 4 cut(s) 298, 484, 827, 846
Bse21I CCTNAGG 2 cut(s) 253, 613
BseBI CCWGG 1 cut(s) 187
BseDI CCNNGG 2 cut(s) 522, 764
BseGI GGATG 3 cut(s) 514, 532, 1029
BseLI CCNNNNNNNGG 2 cut(s) 986, 987
BseMII CTCAG 3 cut(s) 267, 309, 828
BseNI ACTGG 4 cut(s) 298, 484, 827, 846
BseXI GCAGC 3 cut(s) 454, 655, 1051
Bsh1285I CGRYCG 1 cut(s) 64
BshFI GGCC 2 cut(s) 54, 269
BshNI GGYRCC 1 cut(s) 287
BshTI ACCGGT 1 cut(s) 993
BsiEI CGRYCG 1 cut(s) 64
BsiSI CCGG 2 cut(s) 981, 994
BslFI GGGAC 4 cut(s) 360, 689, 736, 845
BslI CCNNNNNNNGG 2 cut(s) 986, 987
BsmAI GTCTC 1 cut(s) 206
BsmBI CGTCTC 1 cut(s) 206
BsmFI GGGAC 4 cut(s) 360, 689, 736, 845
BsmI GAATGC 1 cut(s) 385
BsnI GGCC 2 cut(s) 54, 269
Bsp119I TTCGAA 1 cut(s) 154
Bsp143I GATC 2 cut(s) 61, 700
Bsp19I CCATGG 1 cut(s) 522
BspACI CCGC 1 cut(s) 470
BspANI GGCC 2 cut(s) 54, 269
BspCNI CTCAG 3 cut(s) 266, 310, 827
BspHI TCATGA 1 cut(s) 796
BspLI GGNNCC 3 cut(s) 86, 289, 992
BspMAI CTGCAG 1 cut(s) 673
BspT104I TTCGAA 1 cut(s) 154
BspT107I GGYRCC 1 cut(s) 287
BsrFI RCCGGY 1 cut(s) 993
BsrI ACTGG 4 cut(s) 298, 484, 827, 846
BssAI RCCGGY 1 cut(s) 993
BssECI CCNNGG 2 cut(s) 522, 764
BssMI GATC 2 cut(s) 61, 700
BssT1I CCWWGG 2 cut(s) 522, 764
Bst2UI CCWGG 1 cut(s) 187
Bst6I CTCTTC 2 cut(s) 204, 643
BstBI TTCGAA 1 cut(s) 154
BstC8I GCNNGC 3 cut(s) 856, 1002, 1044
BstDEI CTNAG 4 cut(s) 253, 318, 613, 814
BstDSI CCRYGG 1 cut(s) 522
BstEII GGTNACC 2 cut(s) 246, 975
BstF5I GGATG 3 cut(s) 514, 532, 1029
BstHHI GCGC 1 cut(s) 242
BstKTI GATC 2 cut(s) 64, 703
BstMAI GTCTC 1 cut(s) 206
BstMBI GATC 2 cut(s) 61, 700
BstMCI CGRYCG 1 cut(s) 64
BstMWI GCNNNNNNNGC 1 cut(s) 851
BstNI CCWGG 1 cut(s) 187
BstNSI RCATGY 1 cut(s) 408
BstPI GGTNACC 2 cut(s) 246, 975
BstSCI CCNGG 2 cut(s) 185, 979
BstSFI CTRYAG 1 cut(s) 669
BstV1I GCAGC 3 cut(s) 454, 655, 1051
Bsu36I CCTNAGG 2 cut(s) 253, 613
BsuRI GGCC 2 cut(s) 54, 269
BtgI CCRYGG 1 cut(s) 522
BtsCI GGATG 3 cut(s) 514, 532, 1029
BtsI GCAGTG 2 cut(s) 142, 270
BtsIMutI CAGTG 7 cut(s) 142, 147, 270, 291, 515, 820, 853
Cac8I GCNNGC 3 cut(s) 856, 1002, 1044
CciI TCATGA 1 cut(s) 796
CfoI GCGC 1 cut(s) 242
Cfr10I RCCGGY 1 cut(s) 993
Cfr13I GGNCC 2 cut(s) 651, 908
CseI GACGC 2 cut(s) 223, 993
Csp6I GTAC 3 cut(s) 288, 780, 804
CspAI ACCGGT 1 cut(s) 993
CviAII CATG 8 cut(s) 350, 375, 393, 405, 523, 797, 893, 947
CviJI RGCY 8 cut(s) 54, 80, 171, 269, 792, 818, 1010, 1042
CviKI_1 RGCY 8 cut(s) 54, 80, 171, 269, 792, 818, 1010, 1042
CviQI GTAC 3 cut(s) 288, 780, 804
DdeI CTNAG 4 cut(s) 253, 318, 613, 814
DpnI GATC 2 cut(s) 63, 702
DpnII GATC 2 cut(s) 61, 700
DraIII CACNNNGTG 1 cut(s) 983
DrdI GACNNNNNNGTC 1 cut(s) 683
DseDI GACNNNNNNGTC 1 cut(s) 683
EaeI YGGCCR 1 cut(s) 267
Eam1104I CTCTTC 2 cut(s) 204, 643
EarI CTCTTC 2 cut(s) 204, 643
Eco130I CCWWGG 2 cut(s) 522, 764
Eco47I GGWCC 2 cut(s) 651, 908
Eco57I CTGAAG 2 cut(s) 597, 950
Eco81I CCTNAGG 2 cut(s) 253, 613
Eco91I GGTNACC 2 cut(s) 246, 975
EcoO65I GGTNACC 2 cut(s) 246, 975
EcoRII CCWGG 1 cut(s) 185
EcoT14I CCWWGG 2 cut(s) 522, 764
EcoT22I ATGCAT 1 cut(s) 385
ErhI CCWWGG 2 cut(s) 522, 764
Esp3I CGTCTC 1 cut(s) 206
FaeI CATG 8 cut(s) 353, 378, 396, 408, 526, 800, 896, 950
FaqI GGGAC 4 cut(s) 360, 689, 736, 845
FatI CATG 8 cut(s) 349, 374, 392, 404, 522, 796, 892, 946
Fnu4HI GCNGC 3 cut(s) 468, 669, 1040
FokI GGATG 3 cut(s) 501, 539, 1036
Fsp4HI GCNGC 3 cut(s) 468, 669, 1040
FspBI CTAG 2 cut(s) 47, 1011
GlaI GCGC 1 cut(s) 241
GluI GCNGC 3 cut(s) 468, 669, 1040
HaeIII GGCC 2 cut(s) 54, 269
HapII CCGG 2 cut(s) 981, 994
HgaI GACGC 2 cut(s) 223, 993
HhaI GCGC 1 cut(s) 242
Hin1II CATG 8 cut(s) 353, 378, 396, 408, 526, 800, 896, 950
Hin6I GCGC 1 cut(s) 240
HinP1I GCGC 1 cut(s) 240
HinfI GANTC 6 cut(s) 37, 121, 156, 620, 769, 896
HpaII CCGG 2 cut(s) 981, 994
HphI GGTGA 7 cut(s) 108, 240, 602, 629, 761, 969, 995
Hpy166II GTNNAC 2 cut(s) 883, 961
Hpy188I TCNGA 3 cut(s) 42, 319, 577
Hpy188III TCNNGA 3 cut(s) 255, 797, 1030
Hpy8I GTNNAC 2 cut(s) 883, 961
Hpy99I CGWCG 2 cut(s) 110, 1009
HpyAV CCTTC 3 cut(s) 44, 440, 921
HpyF10VI GCNNNNNNNGC 1 cut(s) 851
HpyF3I CTNAG 4 cut(s) 253, 318, 613, 814
Hsp92II CATG 8 cut(s) 353, 378, 396, 408, 526, 800, 896, 950
HspAI GCGC 1 cut(s) 240
KpnI GGTACC 1 cut(s) 291
Kzo9I GATC 2 cut(s) 61, 700
LmnI GCTCC 3 cut(s) 72, 188, 851
Lsp1109I GCAGC 3 cut(s) 454, 655, 1051
LweI GCATC 3 cut(s) 392, 523, 729
MaeI CTAG 2 cut(s) 47, 1011
MaeIII GTNAC 4 cut(s) 246, 632, 685, 975
MalI GATC 2 cut(s) 63, 702
MboI GATC 2 cut(s) 61, 700
MlsI TGGCCA 1 cut(s) 269
MluCI AATT 1 cut(s) 307
MluNI TGGCCA 1 cut(s) 269
MlyI GAGTC 1 cut(s) 890
MnlI CCTC 4 cut(s) 225, 262, 337, 979
Mox20I TGGCCA 1 cut(s) 269
Mph1103I ATGCAT 1 cut(s) 385
MscI TGGCCA 1 cut(s) 269
MseI TTAA 2 cut(s) 476, 663
MslI CAYNNNNRTG 1 cut(s) 951
Msp20I TGGCCA 1 cut(s) 269
MspI CCGG 2 cut(s) 981, 994
MspR9I CCNGG 2 cut(s) 187, 981
Mva1269I GAATGC 1 cut(s) 385
MvaI CCWGG 1 cut(s) 187
MwoI GCNNNNNNNGC 1 cut(s) 851
NciI CCSGG 1 cut(s) 981
NcoI CCATGG 1 cut(s) 522
NdeII GATC 2 cut(s) 61, 700
NlaIII CATG 8 cut(s) 353, 378, 396, 408, 526, 800, 896, 950
NlaIV GGNNCC 3 cut(s) 86, 289, 992
NmuCI GTSAC 4 cut(s) 246, 632, 685, 975
NsiI ATGCAT 1 cut(s) 385
NspI RCATGY 1 cut(s) 408
NspV TTCGAA 1 cut(s) 154
PagI TCATGA 1 cut(s) 796
PciI ACATGT 1 cut(s) 404
PctI GAATGC 1 cut(s) 385
PfeI GAWTC 5 cut(s) 37, 121, 156, 620, 769
PfoI TCCNGGA 1 cut(s) 185
PinAI ACCGGT 1 cut(s) 993
PkrI GCNGC 3 cut(s) 469, 670, 1041
Ple19I CGATCG 1 cut(s) 64
PleI GAGTC 1 cut(s) 890
PpsI GAGTC 1 cut(s) 890
PscI ACATGT 1 cut(s) 404
Psp6I CCWGG 1 cut(s) 185
PspEI GGTNACC 2 cut(s) 246, 975
PspGI CCWGG 1 cut(s) 185
PspN4I GGNNCC 3 cut(s) 86, 289, 992
PspPI GGNCC 2 cut(s) 651, 908
PstI CTGCAG 1 cut(s) 673
PvuI CGATCG 1 cut(s) 64
RsaI GTAC 3 cut(s) 289, 781, 805
RsaNI GTAC 3 cut(s) 288, 780, 804
RseI CAYNNNNRTG 1 cut(s) 951
SaqAI TTAA 2 cut(s) 476, 663
SatI GCNGC 3 cut(s) 468, 669, 1040
Sau3AI GATC 2 cut(s) 61, 700
Sau96I GGNCC 2 cut(s) 651, 908
SchI GAGTC 1 cut(s) 890
ScrFI CCNGG 2 cut(s) 187, 981
SfaNI GCATC 3 cut(s) 392, 523, 729
SfcI CTRYAG 1 cut(s) 669
SfuI TTCGAA 1 cut(s) 154
SinI GGWCC 2 cut(s) 651, 908
SmiMI CAYNNNNRTG 1 cut(s) 951
Sse9I AATT 1 cut(s) 307
SsiI CCGC 1 cut(s) 470
SspMI CTAG 2 cut(s) 47, 1011
StyD4I CCNGG 2 cut(s) 185, 979
StyI CCWWGG 2 cut(s) 522, 764
TaqI TCGA 1 cut(s) 154
TasI AATT 1 cut(s) 307
TatI WGTACW 1 cut(s) 779
TfiI GAWTC 5 cut(s) 37, 121, 156, 620, 769
Tru1I TTAA 2 cut(s) 476, 663
Tru9I TTAA 2 cut(s) 476, 663
TscAI CASTG 7 cut(s) 142, 154, 277, 298, 515, 827, 853
TseFI GTSAC 4 cut(s) 246, 632, 685, 975
TseI GCWGC 3 cut(s) 467, 668, 1039
Tsp45I GTSAC 4 cut(s) 246, 632, 685, 975
TspDTI ATGAA 6 cut(s) 366, 381, 468, 761, 903, 963
TspRI CASTG 7 cut(s) 142, 154, 277, 298, 515, 827, 853
VpaK11BI GGWCC 2 cut(s) 651, 908
XceI RCATGY 1 cut(s) 408
XspI CTAG 2 cut(s) 47, 1011
Zsp2I ATGCAT 1 cut(s) 385
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.