FvH4_6g18650

Essential component of the cytosolic iron-sulfur (Fe S) protein assembly machinery. Required for the maturation of extramitochondrial Fe S proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0007416
Physical Location & Seq
Reverse (-)
12316288 .. 12320568
4281 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g18650.t1

Sequence Viewer

Length: 1041 bp
ATGGAGACTCTCAAGCAGATTCAGAAGCTCGAGGGCCACACCGACCGGGTCTGGAGCCTCGCGTGGAACCCACTCTCCGGTCTCAACGGCCACCCCCTAAAACTCGCCTCATGCAGCGGCGATAAAACCGTCCGTATCTGGGAACGCAAACCCTCCAACTCCTGGGAATGCCAGGCAGTTCTGGATGATCCGCACAGCAGGACCGTCCGATCGTGCTCTTGGTCCCCCTCCGGTAAAATCCTAGCCACCGCCAGCTTCGACGGCACCACCGCGATTTTCCAGGACGTTGGGGACGATTACGAGTGCGTCTCCACTTTAGAGGGTCACGAGAATGAAGTGAAGAGTGTGTCTTGGAATGCGTCCGGGTCTCTGCTTGCGACTTGCGGGAGGGACAAGACTGTGTGGATATGGGAGGTGTTGCCGGGGAATGAGTTTGAGTGCGTTGCGGTGTTGCAAGGCCATACGCAAGATGTGAAGATGGTTCAGTGGCATCCCTCTGTGGAGCTTTTGTTTTCGTGTAGCTATGACAACACTGTCAAGATATGGGCAGATGATGGTGAGGATGATAACTGGGTGTGTGTGCAAACGTTAGCTCAATCTAACAGTGGTCACTCTTCTACTGTATGGGCTCTATCATTTAATGCTACGGGAGACAAAATGGTTACCTGCAGTGATGATCTTACCCTAAAGATATGGGGAACAGACAACGGGAAGATGCTATCTTCGGATGGCTTTGCACCTTGGAGTCATCTTTGCACTATTGCGGGTTATCATGACAGGACTATATTTTCAGTTCATTGGTCAAGGGACAATATCATTGCCAGTGGAGCTGCTGATGATGCCATAAGATTTTTTGTGGAGAATAATGAGAGCGATAGTTTGGTCAATGGACCTTCCTATAACTTGCTTCTCAAGAAGGAGAAAGCCCACGACATGGATATAAATTCAGTGCAGTGGTGCCCTGGGGAGAATAGGCTTCTTGCTTCTGCAGCTGATGACGGGACGATCAAAATATGGGAGCTGACATCTGTTAGCTGCTGA

Protein Analysis

347

Amino Acids

38.3

Weight (kDa)

4.82

Isoelectric Point (pI)

26.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR5 PF25175 2 - 93 4.5e-12 WDR5 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 3 - 234 2.6e-25 WDR3 second beta-propeller domain
WD40_CDC20-Fz PF24807 3 - 84 6.1e-07 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 4 - 139 6.3e-13 WDHD1 first WD40 domain
WD40_Prp19 PF24814 4 - 139 7.2e-18 Prp19 WD40 domain
WD40 PF00400 5 - 47 5.6e-09 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 5 - 52 2e-07 THOC3 beta-propeller domain
WD40_Gbeta PF25391 8 - 233 6.2e-12 G protein beta WD-40 repeat protein
Beta-prop_Aladin PF25460 16 - 139 1.5e-07 Aladin seven-bladed propeller
Beta-prop_EML_2 PF23414 27 - 183 1.2e-16 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 29 - 114 1.4e-06 CAF1B/HIR1 beta-propeller domain
WD40 PF00400 56 - 92 5.2e-06 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 58 - 232 4.2e-27 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 61 - 242 3.6e-19 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 64 - 241 5.1e-35 WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 65 - 183 2.1e-06 WDR36/Utp21 first beta-propeller
Beta-prop_THOC3 PF25174 65 - 119 5.7e-09 THOC3 beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 66 - 239 4.8e-11 MABP1/WDR62 second WD40 domain
Beta-prop_TEP1_2nd PF25047 97 - 234 5e-12 TEP-1 second beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 100 - 183 2.1e-08 CAF1B/HIR1 beta-propeller domain
WD40_Prp19 PF24814 101 - 167 3.9e-10 Prp19 WD40 domain
WD40 PF00400 101 - 137 1.3e-10 WD domain, G-beta repeat
EIF3I PF24805 105 - 183 3.5e-08 EIF3I
Beta-prop_Aladin PF25460 106 - 213 1.3e-07 Aladin seven-bladed propeller
Beta-prop_THOC3 PF25174 109 - 183 1e-18 THOC3 beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 125 - 240 9e-10 CAF1B/HIR1 beta-propeller domain
WD40_Prp19 PF24814 146 - 241 1.3e-14 Prp19 WD40 domain
WD40 PF00400 146 - 182 1.8e-09 WD domain, G-beta repeat
EIF3I PF24805 150 - 342 7.6e-11 EIF3I
WD40 PF00400 193 - 232 5.9e-07 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 197 - 285 4.3e-08 WDR5 beta-propeller domain
Beta-prop_EML_2 PF23414 208 - 342 7.6e-07 Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz PF24807 234 - 344 5.1e-08 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 258 - 345 7.7e-08 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 258 - 344 1e-09 THOC3 beta-propeller domain
WD40 PF00400 307 - 339 3.6e-06 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 533
Acc36I ACCTGC 1 cut(s) 674
AccB1I GGYRCC 2 cut(s) 263, 957
AccB7I CCANNNNNTGG 2 cut(s) 162, 934
AccII CGCG 2 cut(s) 62, 272
AciI CCGC 7 cut(s) 117, 191, 249, 270, 384, 446, 764
AclI AACGTT 1 cut(s) 587
AclWI GGATC 1 cut(s) 182
AcoI YGGCCR 1 cut(s) 88
AcsI RAATTY 1 cut(s) 943
AfiI CCNNNNNNNGG 4 cut(s) 77, 139, 162, 934
AjnI CCWGG 4 cut(s) 161, 171, 279, 961
AloI GAACNNNNNNTCC 2 cut(s) 59, 91
AluBI AGCT 9 cut(s) 28, 255, 505, 522, 593, 830, 992, 1021, 1035
AluI AGCT 9 cut(s) 28, 255, 505, 522, 593, 830, 992, 1021, 1035
Alw21I GWGCWC 1 cut(s) 218
Alw26I GTCTC 4 cut(s) 86, 313, 372, 645
AlwI GGATC 1 cut(s) 182
Ama87I CYCGRG 1 cut(s) 29
AoxI GGCC 3 cut(s) 34, 88, 457
ApeKI GCWGC 4 cut(s) 114, 830, 989, 1035
ApoI RAATTY 1 cut(s) 943
AspS9I GGNCC 4 cut(s) 34, 201, 222, 890
AsuC2I CCSGG 3 cut(s) 47, 364, 423
AsuHPI GGTGA 1 cut(s) 569
AvaI CYCGRG 1 cut(s) 29
AvaII GGWCC 3 cut(s) 201, 222, 890
BaeGI GKGCMC 1 cut(s) 962
BanI GGYRCC 2 cut(s) 263, 957
BanII GRGCYC 1 cut(s) 631
BauI CACGAG 1 cut(s) 326
Bbv12I GWGCWC 1 cut(s) 218
BbvI GCAGC 4 cut(s) 126, 817, 1001, 1022
BccI CCATC 3 cut(s) 472, 548, 722
BceAI ACGGC 2 cut(s) 103, 277
BciT130I CCWGG 4 cut(s) 163, 173, 281, 963
BcnI CCSGG 3 cut(s) 47, 364, 423
BcoDI GTCTC 4 cut(s) 86, 313, 372, 645
BfaI CTAG 1 cut(s) 242
BfmI CTRYAG 2 cut(s) 667, 987
BfuAI ACCTGC 1 cut(s) 674
BisI GCNGC 5 cut(s) 115, 118, 831, 990, 1036
BlsI GCNGC 5 cut(s) 116, 119, 832, 991, 1037
Bme1390I CCNGG 7 cut(s) 47, 163, 173, 281, 364, 423, 963
Bme18I GGWCC 3 cut(s) 201, 222, 890
BmeT110I CYCGRG 1 cut(s) 29
BmgT120I GGNCC 4 cut(s) 34, 201, 222, 890
BmiI GGNNCC 5 cut(s) 56, 68, 224, 265, 959
BmrFI CCNGG 7 cut(s) 47, 163, 173, 281, 364, 423, 963
BmrI ACTGGG 1 cut(s) 580
BmsI GCATC 3 cut(s) 499, 705, 829
BmuI ACTGGG 1 cut(s) 580
BplI GAGNNNNNCTC 2 cut(s) 293, 325
BpmI CTGGAG 1 cut(s) 73
BpuEI CTTGAG 1 cut(s) 896
BpuMI CCSGG 3 cut(s) 47, 364, 423
BsaI GGTCTC 2 cut(s) 86, 372
BsaJI CCNNGG 5 cut(s) 162, 422, 740, 961, 962
BsaWI WCCGGW 2 cut(s) 77, 230
BsaXI ACNNNNNCTCC 4 cut(s) 59, 89, 494, 524
Bsc4I CCNNNNNNNGG 4 cut(s) 77, 139, 162, 934
Bse1I ACTGG 2 cut(s) 575, 822
Bse3DI GCAATG 1 cut(s) 816
BseBI CCWGG 4 cut(s) 163, 173, 281, 963
BseDI CCNNGG 5 cut(s) 162, 422, 740, 961, 962
BseGI GGATG 4 cut(s) 190, 490, 568, 733
BseLI CCNNNNNNNGG 4 cut(s) 77, 139, 162, 934
BseMI GCAATG 1 cut(s) 816
BseNI ACTGG 2 cut(s) 575, 822
BseSI GKGCMC 1 cut(s) 962
BseXI GCAGC 4 cut(s) 126, 817, 1001, 1022
BsgI GTGCAG 1 cut(s) 971
Bsh1236I CGCG 2 cut(s) 62, 272
Bsh1285I CGRYCG 2 cut(s) 46, 212
BshFI GGCC 3 cut(s) 36, 90, 459
BshNI GGYRCC 2 cut(s) 263, 957
BsiEI CGRYCG 2 cut(s) 46, 212
BsiHKAI GWGCWC 1 cut(s) 218
BsiHKCI CYCGRG 1 cut(s) 29
BsiSI CCGG 5 cut(s) 46, 78, 231, 363, 422
BslFI GGGAC 5 cut(s) 208, 305, 404, 821, 1015
BslI CCNNNNNNNGG 4 cut(s) 77, 139, 162, 934
BsmAI GTCTC 4 cut(s) 86, 313, 372, 645
BsmBI CGTCTC 1 cut(s) 313
BsmFI GGGAC 5 cut(s) 208, 305, 404, 821, 1015
BsmI GAATGC 2 cut(s) 173, 361
BsnI GGCC 3 cut(s) 36, 90, 459
Bso31I GGTCTC 2 cut(s) 86, 372
BsoBI CYCGRG 1 cut(s) 29
Bsp1286I GDGCHC 3 cut(s) 218, 631, 962
Bsp143I GATC 4 cut(s) 187, 209, 676, 1005
BspACI CCGC 7 cut(s) 117, 191, 249, 270, 384, 446, 764
BspANI GGCC 3 cut(s) 36, 90, 459
BspFNI CGCG 2 cut(s) 62, 272
BspHI TCATGA 1 cut(s) 772
BspLI GGNNCC 5 cut(s) 56, 68, 224, 265, 959
BspMAI CTGCAG 2 cut(s) 671, 991
BspMI ACCTGC 1 cut(s) 674
BspPI GGATC 1 cut(s) 182
BspT107I GGYRCC 2 cut(s) 263, 957
BspTNI GGTCTC 2 cut(s) 86, 372
BsrDI GCAATG 1 cut(s) 816
BsrI ACTGG 2 cut(s) 575, 822
BssECI CCNNGG 5 cut(s) 162, 422, 740, 961, 962
BssMI GATC 4 cut(s) 187, 209, 676, 1005
BssSI CACGAG 1 cut(s) 326
BssT1I CCWWGG 1 cut(s) 740
Bst2BI CACGAG 1 cut(s) 326
Bst2UI CCWGG 4 cut(s) 163, 173, 281, 963
Bst4CI ACNGT 6 cut(s) 130, 205, 400, 535, 605, 622
Bst6I CTCTTC 2 cut(s) 335, 619
BstC8I GCNNGC 2 cut(s) 253, 375
BstEII GGTNACC 1 cut(s) 661
BstF5I GGATG 4 cut(s) 190, 490, 568, 733
BstFNI CGCG 2 cut(s) 62, 272
BstKTI GATC 4 cut(s) 190, 212, 679, 1008
BstMAI GTCTC 4 cut(s) 86, 313, 372, 645
BstMBI GATC 4 cut(s) 187, 209, 676, 1005
BstMCI CGRYCG 2 cut(s) 46, 212
BstMWI GCNNNNNNNGC 4 cut(s) 261, 827, 839, 989
BstNI CCWGG 4 cut(s) 163, 173, 281, 963
BstPI GGTNACC 1 cut(s) 661
BstSCI CCNGG 7 cut(s) 45, 161, 171, 279, 362, 421, 961
BstSFI CTRYAG 2 cut(s) 667, 987
BstSLI GKGCMC 1 cut(s) 962
BstUI CGCG 2 cut(s) 62, 272
BstV1I GCAGC 4 cut(s) 126, 817, 1001, 1022
BstXI CCANNNNNNTGG 1 cut(s) 287
BsuRI GGCC 3 cut(s) 36, 90, 459
BtsCI GGATG 4 cut(s) 190, 490, 568, 733
BtsI GCAGTG 2 cut(s) 676, 959
BtsIMutI CAGTG 7 cut(s) 491, 531, 610, 676, 829, 954, 959
BveI ACCTGC 1 cut(s) 674
Cac8I GCNNGC 2 cut(s) 253, 375
CciI TCATGA 1 cut(s) 772
Cfr13I GGNCC 4 cut(s) 34, 201, 222, 890
CseI GACGC 2 cut(s) 295, 348
CviAII CATG 3 cut(s) 111, 773, 934
DpnI GATC 4 cut(s) 189, 211, 678, 1007
DpnII GATC 4 cut(s) 187, 209, 676, 1005
DrdI GACNNNNNNGTC 1 cut(s) 533
DseDI GACNNNNNNGTC 1 cut(s) 533
EaeI YGGCCR 1 cut(s) 88
Eam1104I CTCTTC 2 cut(s) 335, 619
EarI CTCTTC 2 cut(s) 335, 619
Eco130I CCWWGG 1 cut(s) 740
Eco24I GRGCYC 1 cut(s) 631
Eco31I GGTCTC 2 cut(s) 86, 372
Eco47I GGWCC 3 cut(s) 201, 222, 890
Eco88I CYCGRG 1 cut(s) 29
Eco91I GGTNACC 1 cut(s) 661
EcoO65I GGTNACC 1 cut(s) 661
EcoRII CCWGG 4 cut(s) 161, 171, 279, 961
EcoT14I CCWWGG 1 cut(s) 740
EcoT38I GRGCYC 1 cut(s) 631
ErhI CCWWGG 1 cut(s) 740
Esp3I CGTCTC 1 cut(s) 313
FaeI CATG 3 cut(s) 114, 776, 937
FaqI GGGAC 5 cut(s) 208, 305, 404, 821, 1015
FatI CATG 3 cut(s) 110, 772, 933
FauI CCCGC 2 cut(s) 377, 757
Fnu4HI GCNGC 5 cut(s) 115, 118, 831, 990, 1036
FokI GGATG 4 cut(s) 197, 477, 575, 740
FriOI GRGCYC 1 cut(s) 631
Fsp4HI GCNGC 5 cut(s) 115, 118, 831, 990, 1036
FspBI CTAG 1 cut(s) 242
GluI GCNGC 5 cut(s) 115, 118, 831, 990, 1036
GsuI CTGGAG 1 cut(s) 73
HaeIII GGCC 3 cut(s) 36, 90, 459
HapII CCGG 5 cut(s) 46, 78, 231, 363, 422
HgaI GACGC 2 cut(s) 295, 348
Hin1II CATG 3 cut(s) 114, 776, 937
HinfI GANTC 3 cut(s) 7, 19, 745
HpaII CCGG 5 cut(s) 46, 78, 231, 363, 422
HphI GGTGA 1 cut(s) 569
Hpy188I TCNGA 3 cut(s) 24, 209, 727
Hpy188III TCNNGA 6 cut(s) 52, 182, 326, 538, 773, 913
Hpy99I CGWCG 1 cut(s) 263
HpyAV CCTTC 2 cut(s) 903, 910
HpyCH4III ACNGT 6 cut(s) 130, 205, 400, 535, 605, 622
HpyCH4IV ACGT 2 cut(s) 285, 587
HpyCH4V TGCA 8 cut(s) 114, 454, 583, 669, 737, 756, 952, 989
HpyF10VI GCNNNNNNNGC 4 cut(s) 261, 827, 839, 989
HpySE526I ACGT 2 cut(s) 285, 587
Hsp92II CATG 3 cut(s) 114, 776, 937
Kzo9I GATC 4 cut(s) 187, 209, 676, 1005
LmnI GCTCC 4 cut(s) 54, 502, 827, 1018
Lsp1109I GCAGC 4 cut(s) 126, 817, 1001, 1022
LweI GCATC 3 cut(s) 499, 705, 829
MaeI CTAG 1 cut(s) 242
MaeII ACGT 2 cut(s) 285, 587
MaeIII GTNAC 3 cut(s) 323, 608, 661
MalI GATC 4 cut(s) 189, 211, 678, 1007
MboI GATC 4 cut(s) 187, 209, 676, 1005
MboII GAAGA 5 cut(s) 352, 487, 606, 714, 724
MhlI GDGCHC 3 cut(s) 218, 631, 962
MluCI AATT 1 cut(s) 943
MlyI GAGTC 1 cut(s) 754
MmeI TCCRAC 1 cut(s) 180
MseI TTAA 1 cut(s) 639
MslI CAYNNNNRTG 1 cut(s) 330
MspA1I CMGCKG 2 cut(s) 117, 992
MspI CCGG 5 cut(s) 46, 78, 231, 363, 422
MspR9I CCNGG 7 cut(s) 47, 163, 173, 281, 364, 423, 963
Mva1269I GAATGC 2 cut(s) 173, 361
MvaI CCWGG 4 cut(s) 163, 173, 281, 963
MvnI CGCG 2 cut(s) 62, 272
MwoI GCNNNNNNNGC 4 cut(s) 261, 827, 839, 989
NciI CCSGG 3 cut(s) 47, 364, 423
NdeII GATC 4 cut(s) 187, 209, 676, 1005
NlaIII CATG 3 cut(s) 114, 776, 937
NlaIV GGNNCC 5 cut(s) 56, 68, 224, 265, 959
NmuCI GTSAC 2 cut(s) 323, 608
PaeR7I CTCGAG 1 cut(s) 29
PagI TCATGA 1 cut(s) 772
PasI CCCWGGG 1 cut(s) 962
PcsI WCGNNNNNNNCGW 1 cut(s) 291
PctI GAATGC 2 cut(s) 173, 361
PfeI GAWTC 1 cut(s) 19
PflFI GACNNNGTC 1 cut(s) 47
PflMI CCANNNNNTGG 2 cut(s) 162, 934
PfoI TCCNGGA 1 cut(s) 279
PkrI GCNGC 5 cut(s) 116, 119, 832, 991, 1037
Ple19I CGATCG 1 cut(s) 212
PleI GAGTC 1 cut(s) 753
PpsI GAGTC 1 cut(s) 753
Psp1406I AACGTT 1 cut(s) 587
Psp6I CCWGG 4 cut(s) 161, 171, 279, 961
PspEI GGTNACC 1 cut(s) 661
PspGI CCWGG 4 cut(s) 161, 171, 279, 961
PspN4I GGNNCC 5 cut(s) 56, 68, 224, 265, 959
PspPI GGNCC 4 cut(s) 34, 201, 222, 890
PspXI VCTCGAGB 1 cut(s) 29
PstI CTGCAG 2 cut(s) 671, 991
PsyI GACNNNGTC 1 cut(s) 47
PvuI CGATCG 1 cut(s) 212
PvuII CAGCTG 1 cut(s) 992
RseI CAYNNNNRTG 1 cut(s) 330
SaqAI TTAA 1 cut(s) 639
SatI GCNGC 5 cut(s) 115, 118, 831, 990, 1036
Sau3AI GATC 4 cut(s) 187, 209, 676, 1005
Sau96I GGNCC 4 cut(s) 34, 201, 222, 890
SchI GAGTC 1 cut(s) 754
ScrFI CCNGG 7 cut(s) 47, 163, 173, 281, 364, 423, 963
SduI GDGCHC 3 cut(s) 218, 631, 962
SfaNI GCATC 3 cut(s) 499, 705, 829
SfcI CTRYAG 2 cut(s) 667, 987
Sfr274I CTCGAG 1 cut(s) 29
SinI GGWCC 3 cut(s) 201, 222, 890
SlaI CTCGAG 1 cut(s) 29
SmiMI CAYNNNNRTG 1 cut(s) 330
SmlI CTYRAG 3 cut(s) 11, 29, 911
SmoI CTYRAG 3 cut(s) 11, 29, 911
Sse9I AATT 1 cut(s) 943
SsiI CCGC 7 cut(s) 117, 191, 249, 270, 384, 446, 764
SspMI CTAG 1 cut(s) 242
StyD4I CCNGG 7 cut(s) 45, 161, 171, 279, 362, 421, 961
StyI CCWWGG 1 cut(s) 740
TaaI ACNGT 6 cut(s) 130, 205, 400, 535, 605, 622
TaiI ACGT 2 cut(s) 288, 590
TaqI TCGA 2 cut(s) 30, 258
TasI AATT 1 cut(s) 943
TauI GCSGC 1 cut(s) 120
TfiI GAWTC 1 cut(s) 19
Tru1I TTAA 1 cut(s) 639
Tru9I TTAA 1 cut(s) 639
TscAI CASTG 7 cut(s) 491, 538, 610, 676, 829, 954, 959
TseFI GTSAC 2 cut(s) 323, 608
TseI GCWGC 4 cut(s) 114, 830, 989, 1035
Tsp45I GTSAC 2 cut(s) 323, 608
TspDTI ATGAA 2 cut(s) 348, 785
TspGWI ACGGA 1 cut(s) 122
TspRI CASTG 7 cut(s) 491, 538, 610, 676, 829, 954, 959
Tth111I GACNNNGTC 1 cut(s) 47
Van91I CCANNNNNTGG 2 cut(s) 162, 934
VpaK11BI GGWCC 3 cut(s) 201, 222, 890
XapI RAATTY 1 cut(s) 943
XhoI CTCGAG 1 cut(s) 29
XspI CTAG 1 cut(s) 242
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.