MD12G1035900.v1.1

Essential component of the cytosolic iron-sulfur (Fe S) protein assembly machinery. Required for the maturation of extramitochondrial Fe S proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0007416
Physical Location & Seq
Reverse (-)
3893943 .. 3898283
4341 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1035900.v1.1.491

Sequence Viewer

Length: 1044 bp
ATGGAGTTAGCAAAAGAGCTGCGAGAGATTCAGAAGCTAGAGGGCCACACTGATAGGGTCTGGAGCCTCGCCTGGAATCCGGCCACCGGTGTCGCCGGAATTCCCCTTTCCTTCGCTTCCTGCAGCGGCGATAAGACCGTTCGTATTTGGGAACAAAACCTATCCACTTCTTCTTGGGATTGTAAGGCAGTTCTGGATGAAACGCACACCAGAACCGTCCGATCGTGCGCTTGGTCCCCATCTGGGAAGTTATTGGCCACTGCAAGCTTCGATGCCACCACTGCAATTTGGGAAAATGTTGGAGGCGATTACGAATGTGTTGCCAGTCTAGAGGGTCATGAAAACGAAGTGAAAAGTGTGTGTTGGAATCCATCTGGGTCTATGCTTACGACTTGCGGGCGGGATAAAACTGTATGGGTATGGGAAGCGCTACCCGGGAATGAGTTCGACTGTGTTTCAGTGTTGCAAGGCCATACACAGGATGTGAAGATGGTTCAGTGGCATCCCACTAGGAATCTTATATTTTCTTGTAGCTATGATAACACTGTCAAGATATGGGCAGATGATGGTGATGATGATGATTGGGCGTGTGTTCAGACATTAGGTGAAACTAACAATGGTCACTCTTCTACCATCTGGGCTCTATCGTTTAATGCCAGTGGAGACAAAATGGTCACCTGCAGTGATGATCTTACTTTAAAGATATGGGGATCAGACGATGCAAAGATGTCTGCTGATGGCTTTGCACCTTGGCGACATCTTTGCACTCTTTCAGGTTATCATGACCGGACAATATTTTCAGTTCATTGGTCAAGGGACAATATCATAGCCAGTGGAGCTGCTGATGATGCCATAAGATTTTTTGTGGAGAACGATGATCAAGATGGTTTGGTTGATGGACCTTCGTATAAATTGCTTTTGAGGAAGGAGAATGCCCATGACATGGATATCAATTCTGTGCAATGGAGCCCTGGGGAGGACCGGGTTCTTGCTTCTGCAGCCGATGACGGGACGATTAAAATATGGGAGCTGACATCTGAATAG

Protein Analysis

348

Amino Acids

38.45

Weight (kDa)

4.59

Isoelectric Point (pI)

27.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR5 PF25175 5 - 99 1.4e-13 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 8 - 98 5.6e-08 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 8 - 53 1.7e-06 THOC3 beta-propeller domain
WD40_Prp19 PF24814 8 - 98 1.9e-10 Prp19 WD40 domain
WD40 PF00400 9 - 50 6.3e-08 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 9 - 98 4.9e-07 WDR3 first beta-propeller domain
Beta-prop_WDR3_2nd PF25172 10 - 238 4.7e-24 WDR3 second beta-propeller domain
WD40_Gbeta PF25391 12 - 236 3.2e-10 G protein beta WD-40 repeat protein
Beta-prop_EML_2 PF23414 20 - 187 2.8e-15 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_TEP1_2nd PF25047 29 - 98 7.3e-06 TEP-1 second beta-propeller
WD40_MABP1-WDR62_2nd PF24782 35 - 288 3.9e-08 MABP1/WDR62 second WD40 domain
Beta-prop_CAF1B_HIR1 PF24105 36 - 142 9.8e-09 CAF1B/HIR1 beta-propeller domain
WD40_WDHD1_1st PF24817 55 - 124 2.3e-07 WDHD1 first WD40 domain
WD40 PF00400 61 - 97 1.1e-06 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 65 - 236 4e-26 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 67 - 236 6e-34 WDR5 beta-propeller domain
WD40_Prp19 PF24814 67 - 143 1.1e-10 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 69 - 147 4.8e-18 THOC3 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 103 - 187 2.7e-07 TEP-1 second beta-propeller
WD40_CDC20-Fz PF24807 104 - 186 4.7e-11 CDC20/Fizzy WD40 domain
Beta-prop_CAF1B_HIR1 PF24105 104 - 186 4.2e-08 CAF1B/HIR1 beta-propeller domain
WD40 PF00400 105 - 141 7.7e-11 WD domain, G-beta repeat
WD40_Prp19 PF24814 105 - 186 1.6e-14 Prp19 WD40 domain
Beta-prop_Aladin PF25460 114 - 189 7e-08 Aladin seven-bladed propeller
Beta-prop_THOC3 PF25174 115 - 187 1.2e-16 THOC3 beta-propeller domain
WD40_Prp19 PF24814 129 - 237 2.4e-16 Prp19 WD40 domain
WD40 PF00400 150 - 186 5.4e-10 WD domain, G-beta repeat
WD40_WDHD1_1st PF24817 151 - 284 4.8e-06 WDHD1 first WD40 domain
EIF3I PF24805 154 - 345 1.8e-08 EIF3I
WD40 PF00400 197 - 236 7e-07 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 201 - 288 5.9e-09 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 204 - 288 5.2e-06 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 208 - 288 3e-08 THOC3 beta-propeller domain
Beta-prop_EML_2 PF23414 212 - 346 1.8e-06 Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz PF24807 259 - 345 9.2e-06 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 261 - 346 1.2e-09 THOC3 beta-propeller domain
WD40 PF00400 309 - 342 1.9e-06 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 686
AasI GACNNNNNNGTC 1 cut(s) 671
Acc36I ACCTGC 1 cut(s) 686
AccB7I CCANNNNNTGG 1 cut(s) 943
AciI CCGC 3 cut(s) 126, 396, 400
AclWI GGATC 1 cut(s) 718
AcoI YGGCCR 2 cut(s) 81, 255
AcsI RAATTY 1 cut(s) 99
AfeI AGCGCT 1 cut(s) 429
AfiI CCNNNNNNNGG 6 cut(s) 86, 243, 478, 943, 976, 1008
AgeI ACCGGT 1 cut(s) 86
AjnI CCWGG 2 cut(s) 71, 970
AluBI AGCT 6 cut(s) 19, 37, 267, 534, 839, 1030
AluI AGCT 6 cut(s) 19, 37, 267, 534, 839, 1030
Alw26I GTCTC 1 cut(s) 657
AlwI GGATC 1 cut(s) 718
Ama87I CYCGRG 1 cut(s) 434
Aor51HI AGCGCT 1 cut(s) 429
AoxI GGCC 4 cut(s) 43, 81, 255, 469
ApeKI GCWGC 4 cut(s) 19, 123, 839, 998
ApoI RAATTY 1 cut(s) 99
AsiGI ACCGGT 1 cut(s) 86
Asp700I GAANNNNTTC 1 cut(s) 443
AspLEI GCGC 2 cut(s) 230, 430
AspS9I GGNCC 4 cut(s) 43, 234, 899, 979
AsuC2I CCSGG 3 cut(s) 435, 436, 983
AsuHPI GGTGA 3 cut(s) 581, 617, 667
AvaI CYCGRG 1 cut(s) 434
AvaII GGWCC 3 cut(s) 234, 899, 979
BalI TGGCCA 1 cut(s) 257
BanII GRGCYC 2 cut(s) 643, 971
BbvI GCAGC 4 cut(s) 6, 135, 826, 1010
BccI CCATC 8 cut(s) 247, 379, 484, 560, 641, 731, 878, 890
BcgI CGANNNNNNTGC 4 cut(s) 302, 336, 744, 778
BciT130I CCWGG 2 cut(s) 73, 972
BclI TGATCA 1 cut(s) 877
BcnI CCSGG 3 cut(s) 435, 436, 983
BcoDI GTCTC 1 cut(s) 657
BfaI CTAG 3 cut(s) 38, 329, 510
BfmI CTRYAG 3 cut(s) 121, 679, 996
BfoI RGCGCY 1 cut(s) 431
BfuAI ACCTGC 1 cut(s) 686
BisI GCNGC 5 cut(s) 20, 124, 127, 840, 999
BlsI GCNGC 5 cut(s) 21, 125, 128, 841, 1000
Bme1390I CCNGG 5 cut(s) 73, 435, 436, 972, 983
Bme18I GGWCC 3 cut(s) 234, 899, 979
BmeT110I CYCGRG 1 cut(s) 434
BmgT120I GGNCC 4 cut(s) 43, 234, 899, 979
BmiI GGNNCC 3 cut(s) 65, 236, 968
BmrFI CCNGG 5 cut(s) 73, 435, 436, 972, 983
BmsI GCATC 4 cut(s) 262, 511, 709, 838
BpmI CTGGAG 1 cut(s) 82
BpuMI CCSGG 3 cut(s) 435, 436, 983
BsaJI CCNNGG 4 cut(s) 434, 749, 970, 971
BsaWI WCCGGW 2 cut(s) 86, 786
Bsc4I CCNNNNNNNGG 6 cut(s) 86, 243, 478, 943, 976, 1008
Bse118I RCCGGY 1 cut(s) 86
Bse1I ACTGG 3 cut(s) 324, 657, 831
Bse3DI GCAATG 1 cut(s) 968
BseBI CCWGG 2 cut(s) 73, 972
BseDI CCNNGG 4 cut(s) 434, 749, 970, 971
BseGI GGATG 3 cut(s) 202, 487, 502
BseLI CCNNNNNNNGG 6 cut(s) 86, 243, 478, 943, 976, 1008
BseMI GCAATG 1 cut(s) 968
BseNI ACTGG 3 cut(s) 324, 657, 831
BseXI GCAGC 4 cut(s) 6, 135, 826, 1010
Bsh1285I CGRYCG 1 cut(s) 224
BshFI GGCC 4 cut(s) 45, 83, 257, 471
BshTI ACCGGT 1 cut(s) 86
BsiEI CGRYCG 1 cut(s) 224
BsiHKCI CYCGRG 1 cut(s) 434
BsiSI CCGG 6 cut(s) 80, 87, 96, 435, 787, 982
BslFI GGGAC 3 cut(s) 220, 830, 1024
BslI CCNNNNNNNGG 6 cut(s) 86, 243, 478, 943, 976, 1008
BsmAI GTCTC 1 cut(s) 657
BsmFI GGGAC 3 cut(s) 220, 830, 1024
BsmI GAATGC 1 cut(s) 937
BsnI GGCC 4 cut(s) 45, 83, 257, 471
BsoBI CYCGRG 1 cut(s) 434
Bsp1286I GDGCHC 2 cut(s) 643, 971
Bsp143I GATC 4 cut(s) 221, 688, 710, 877
BspACI CCGC 3 cut(s) 126, 396, 400
BspANI GGCC 4 cut(s) 45, 83, 257, 471
BspHI TCATGA 2 cut(s) 337, 781
BspLI GGNNCC 3 cut(s) 65, 236, 968
BspMAI CTGCAG 3 cut(s) 125, 683, 1000
BspMI ACCTGC 1 cut(s) 686
BspPI GGATC 1 cut(s) 718
BsrDI GCAATG 1 cut(s) 968
BsrFI RCCGGY 1 cut(s) 86
BsrI ACTGG 3 cut(s) 324, 657, 831
BssAI RCCGGY 1 cut(s) 86
BssECI CCNNGG 4 cut(s) 434, 749, 970, 971
BssMI GATC 4 cut(s) 221, 688, 710, 877
BssT1I CCWWGG 1 cut(s) 749
Bst2UI CCWGG 2 cut(s) 73, 972
Bst4CI ACNGT 5 cut(s) 139, 217, 412, 452, 547
Bst6I CTCTTC 1 cut(s) 631
BstC8I GCNNGC 2 cut(s) 265, 398
BstEII GGTNACC 1 cut(s) 673
BstF5I GGATG 3 cut(s) 202, 487, 502
BstH2I RGCGCY 1 cut(s) 431
BstHHI GCGC 2 cut(s) 230, 430
BstKTI GATC 4 cut(s) 224, 691, 713, 880
BstMAI GTCTC 1 cut(s) 657
BstMBI GATC 4 cut(s) 221, 688, 710, 877
BstMCI CGRYCG 1 cut(s) 224
BstMWI GCNNNNNNNGC 4 cut(s) 281, 836, 848, 998
BstNI CCWGG 2 cut(s) 73, 972
BstPI GGTNACC 1 cut(s) 673
BstSCI CCNGG 5 cut(s) 71, 433, 434, 970, 981
BstSFI CTRYAG 3 cut(s) 121, 679, 996
BstV1I GCAGC 4 cut(s) 6, 135, 826, 1010
BsuRI GGCC 4 cut(s) 45, 83, 257, 471
BtsCI GGATG 3 cut(s) 202, 487, 502
BtsI GCAGTG 3 cut(s) 258, 279, 688
BtsIMutI CAGTG 9 cut(s) 48, 258, 279, 465, 503, 543, 664, 688, 838
BveI ACCTGC 1 cut(s) 686
Cac8I GCNNGC 2 cut(s) 265, 398
CciI TCATGA 2 cut(s) 337, 781
CfoI GCGC 2 cut(s) 230, 430
Cfr10I RCCGGY 1 cut(s) 86
Cfr13I GGNCC 4 cut(s) 43, 234, 899, 979
Cfr9I CCCGGG 1 cut(s) 434
CspAI ACCGGT 1 cut(s) 86
CviAII CATG 4 cut(s) 338, 782, 938, 943
DpnI GATC 4 cut(s) 223, 690, 712, 879
DpnII GATC 4 cut(s) 221, 688, 710, 877
DraI TTTAAA 1 cut(s) 699
DrdI GACNNNNNNGTC 1 cut(s) 671
DseDI GACNNNNNNGTC 1 cut(s) 671
EaeI YGGCCR 2 cut(s) 81, 255
Eam1104I CTCTTC 1 cut(s) 631
EarI CTCTTC 1 cut(s) 631
Eco130I CCWWGG 1 cut(s) 749
Eco24I GRGCYC 2 cut(s) 643, 971
Eco32I GATATC 1 cut(s) 949
Eco47I GGWCC 3 cut(s) 234, 899, 979
Eco47III AGCGCT 1 cut(s) 429
Eco88I CYCGRG 1 cut(s) 434
Eco91I GGTNACC 1 cut(s) 673
EcoO65I GGTNACC 1 cut(s) 673
EcoRI GAATTC 1 cut(s) 99
EcoRII CCWGG 2 cut(s) 71, 970
EcoRV GATATC 1 cut(s) 949
EcoT14I CCWWGG 1 cut(s) 749
EcoT38I GRGCYC 2 cut(s) 643, 971
ErhI CCWWGG 1 cut(s) 749
FaeI CATG 4 cut(s) 341, 785, 941, 946
FaqI GGGAC 3 cut(s) 220, 830, 1024
FatI CATG 4 cut(s) 337, 781, 937, 942
FauI CCCGC 2 cut(s) 389, 393
FbaI TGATCA 1 cut(s) 877
Fnu4HI GCNGC 5 cut(s) 20, 124, 127, 840, 999
FokI GGATG 3 cut(s) 209, 489, 494
FriOI GRGCYC 2 cut(s) 643, 971
Fsp4HI GCNGC 5 cut(s) 20, 124, 127, 840, 999
FspBI CTAG 3 cut(s) 38, 329, 510
GlaI GCGC 2 cut(s) 229, 429
GluI GCNGC 5 cut(s) 20, 124, 127, 840, 999
GsuI CTGGAG 1 cut(s) 82
HaeII RGCGCY 1 cut(s) 431
HaeIII GGCC 4 cut(s) 45, 83, 257, 471
HapII CCGG 6 cut(s) 80, 87, 96, 435, 787, 982
HhaI GCGC 2 cut(s) 230, 430
Hin1II CATG 4 cut(s) 341, 785, 941, 946
Hin6I GCGC 2 cut(s) 228, 428
HinP1I GCGC 2 cut(s) 228, 428
HindIII AAGCTT 1 cut(s) 265
HinfI GANTC 4 cut(s) 28, 76, 367, 514
HpaII CCGG 6 cut(s) 80, 87, 96, 435, 787, 982
HphI GGTGA 3 cut(s) 581, 617, 667
Hpy188I TCNGA 5 cut(s) 33, 221, 597, 715, 1039
Hpy188III TCNNGA 7 cut(s) 61, 194, 329, 338, 550, 782, 881
HpyAV CCTTC 3 cut(s) 121, 912, 919
HpyCH4III ACNGT 5 cut(s) 139, 217, 412, 452, 547
HpyF10VI GCNNNNNNNGC 4 cut(s) 281, 836, 848, 998
Hsp92II CATG 4 cut(s) 341, 785, 941, 946
HspAI GCGC 2 cut(s) 228, 428
Ksp22I TGATCA 1 cut(s) 877
Kzo9I GATC 4 cut(s) 221, 688, 710, 877
LmnI GCTCC 4 cut(s) 63, 836, 966, 1027
Lsp1109I GCAGC 4 cut(s) 6, 135, 826, 1010
LweI GCATC 4 cut(s) 262, 511, 709, 838
MaeI CTAG 3 cut(s) 38, 329, 510
MaeIII GTNAC 2 cut(s) 620, 673
MalI GATC 4 cut(s) 223, 690, 712, 879
MboI GATC 4 cut(s) 221, 688, 710, 877
MboII GAAGA 3 cut(s) 162, 499, 618
MhlI GDGCHC 2 cut(s) 643, 971
MlsI TGGCCA 1 cut(s) 257
MluCI AATT 4 cut(s) 99, 285, 911, 952
MluNI TGGCCA 1 cut(s) 257
MmeI TCCRAC 2 cut(s) 280, 344
MnlI CCTC 6 cut(s) 34, 77, 296, 325, 915, 970
Mox20I TGGCCA 1 cut(s) 257
MroXI GAANNNNTTC 1 cut(s) 443
MscI TGGCCA 1 cut(s) 257
MseI TTAA 3 cut(s) 651, 698, 1017
Msp20I TGGCCA 1 cut(s) 257
MspA1I CMGCKG 1 cut(s) 126
MspI CCGG 6 cut(s) 80, 87, 96, 435, 787, 982
MspR9I CCNGG 5 cut(s) 73, 435, 436, 972, 983
Mva1269I GAATGC 1 cut(s) 937
MvaI CCWGG 2 cut(s) 73, 972
MwoI GCNNNNNNNGC 4 cut(s) 281, 836, 848, 998
NciI CCSGG 3 cut(s) 435, 436, 983
NdeII GATC 4 cut(s) 221, 688, 710, 877
NlaIII CATG 4 cut(s) 341, 785, 941, 946
NlaIV GGNNCC 3 cut(s) 65, 236, 968
NmuCI GTSAC 2 cut(s) 620, 673
PagI TCATGA 2 cut(s) 337, 781
PaqCI CACCTGC 1 cut(s) 686
PasI CCCWGGG 1 cut(s) 971
PctI GAATGC 1 cut(s) 937
PdmI GAANNNNTTC 1 cut(s) 443
PfeI GAWTC 4 cut(s) 28, 76, 367, 514
PflMI CCANNNNNTGG 1 cut(s) 943
PinAI ACCGGT 1 cut(s) 86
PkrI GCNGC 5 cut(s) 21, 125, 128, 841, 1000
Ple19I CGATCG 1 cut(s) 224
Psp6I CCWGG 2 cut(s) 71, 970
PspEI GGTNACC 1 cut(s) 673
PspGI CCWGG 2 cut(s) 71, 970
PspN4I GGNNCC 3 cut(s) 65, 236, 968
PspPI GGNCC 4 cut(s) 43, 234, 899, 979
PstI CTGCAG 3 cut(s) 125, 683, 1000
PvuI CGATCG 1 cut(s) 224
SaqAI TTAA 3 cut(s) 651, 698, 1017
SatI GCNGC 5 cut(s) 20, 124, 127, 840, 999
Sau3AI GATC 4 cut(s) 221, 688, 710, 877
Sau96I GGNCC 4 cut(s) 43, 234, 899, 979
ScrFI CCNGG 5 cut(s) 73, 435, 436, 972, 983
SduI GDGCHC 2 cut(s) 643, 971
SfaNI GCATC 4 cut(s) 262, 511, 709, 838
SfcI CTRYAG 3 cut(s) 121, 679, 996
SgrAI CRCCGGYG 1 cut(s) 86
SinI GGWCC 3 cut(s) 234, 899, 979
SmaI CCCGGG 1 cut(s) 436
Sse9I AATT 4 cut(s) 99, 285, 911, 952
SsiI CCGC 3 cut(s) 126, 396, 400
SspI AATATT 1 cut(s) 795
SspMI CTAG 3 cut(s) 38, 329, 510
StyD4I CCNGG 5 cut(s) 71, 433, 434, 970, 981
StyI CCWWGG 1 cut(s) 749
TaaI ACNGT 5 cut(s) 139, 217, 412, 452, 547
TaqI TCGA 2 cut(s) 270, 447
TasI AATT 4 cut(s) 99, 285, 911, 952
TauI GCSGC 1 cut(s) 129
TfiI GAWTC 4 cut(s) 28, 76, 367, 514
Tru1I TTAA 3 cut(s) 651, 698, 1017
Tru9I TTAA 3 cut(s) 651, 698, 1017
TscAI CASTG 9 cut(s) 55, 265, 286, 465, 503, 550, 664, 688, 838
TseFI GTSAC 2 cut(s) 620, 673
TseI GCWGC 4 cut(s) 19, 123, 839, 998
Tsp45I GTSAC 2 cut(s) 620, 673
TspDTI ATGAA 3 cut(s) 213, 354, 794
TspMI CCCGGG 1 cut(s) 434
TspRI CASTG 9 cut(s) 55, 265, 286, 465, 503, 550, 664, 688, 838
Van91I CCANNNNNTGG 1 cut(s) 943
VpaK11BI GGWCC 3 cut(s) 234, 899, 979
XapI RAATTY 1 cut(s) 99
XbaI TCTAGA 1 cut(s) 328
XmaI CCCGGG 1 cut(s) 434
XmnI GAANNNNTTC 1 cut(s) 443
XspI CTAG 3 cut(s) 38, 329, 510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.