MD14G1022600.v1.1

Essential component of the cytosolic iron-sulfur (Fe S) protein assembly machinery. Required for the maturation of extramitochondrial Fe S proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0007416
Physical Location & Seq
Reverse (-)
2051078 .. 2054946
3869 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1022600.v1.1.491

Sequence Viewer

Length: 1050 bp
ATGGAGTTAGTGAAGGAGCTCCGAGAGATTCAGACGCTACAGGGCCACACTGATAGGGTATGGAGCCTCGCCTGGAATCCGGCCACGGGTGTCGCTGGAATCCCCCTTTCCTTCGCTTCCTGCAGCGGCGATAAGACCGTTCGTATTTGGGAACAAAACCCCTCAACTTCTTCTTGGGATTGCAAGGCAGTCCTGGATGAAACACACACCAGAACCGTCCGATCGTGCGCTTGGTCCCCATCTGGGAAGTTATTGGCCACTGCAAGCTTCGATGCCACTACTGCAATTTGGGAAAATGTTGGGGGCGATTATGAATGTGTTGCCAGTCTAGAGGGTCATGAAAACGAAGTGAAAAGTGTGTGTTGGAATCCATCTGGGTCTATGCTTACGACTTGCGGTCGAGATAAAAGTGTATGGGTATGGGAAGTGCTGCCCGGGAACGAGTTCGACTGTGTTTCAGTTTTGCAAGGCCATACACAAGATGTGAAGATGGTTCAGTGGCATCCCACTAGGAATCTTATATTTTCTTGTAGCTATGATAACACTGTCAAGATATGGGCTGATGATGGTGACGATGATGATTGGGCATGTGTTCAAACATTAGGTGAAACTAACAATGGTCACTCTTCTACTGTCTGGGCTCTATCATTTAATGCCAGTGGAGACAAGATGGTTACCTGCAGTGATGATCTTACTCTAAAGATATGGGGATCAGACGATACAAAGATGCCTGCTGATGGTTTTGCACCTTGGCGACATCTTTGCACTCTTTCAGGTTATCATGACCGGACAATATTTTCAGTTCATTGGTCAAGGGACAATATCATAGCCAGTGGAGCTGCAGATGATGCAATAAGATTTTTCGTGGAGAATGATGAACAAGATGGTTTGGTTGATGGGCCTTCATATAAATTGCTTTTGAGGAAGGAAAATGCCCATGACATGGATATCAATTCGGTGCAATGGAGCCCCGGGGAGGATCAGTTTCTTGCTTCTGCAGCGGATGACGGGACGATTAAAATATGGGAGCTGACATCTGATAGCTGCTAA

Protein Analysis

350

Amino Acids

38.69

Weight (kDa)

4.52

Isoelectric Point (pI)

30.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR5 PF25175 5 - 99 5.7e-14 WDR5 beta-propeller domain
WD40_WDHD1_1st PF24817 6 - 143 9.3e-12 WDHD1 first WD40 domain
WD40_Gbeta PF25391 7 - 236 7.1e-11 G protein beta WD-40 repeat protein
WD40_CDC20-Fz PF24807 8 - 98 4.4e-08 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 8 - 143 8.4e-17 Prp19 WD40 domain
WD40 PF00400 9 - 50 1.9e-08 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 9 - 54 1.2e-06 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 12 - 238 2.7e-23 WDR3 second beta-propeller domain
Beta-prop_EML_2 PF23414 20 - 187 4.1e-16 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 37 - 186 4.3e-14 CAF1B/HIR1 beta-propeller domain
WD40 PF00400 61 - 97 1.4e-06 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 67 - 240 4.2e-34 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 67 - 236 1.3e-26 WDR3 first beta-propeller domain
Beta-prop_EML PF23409 68 - 284 1.1e-09 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_THOC3 PF25174 69 - 148 1e-17 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 69 - 239 2.1e-16 CDC20/Fizzy WD40 domain
Beta-prop_TEP1_2nd PF25047 104 - 187 2.3e-08 TEP-1 second beta-propeller
WD40 PF00400 105 - 141 2.2e-10 WD domain, G-beta repeat
WD40_Prp19 PF24814 105 - 186 4.3e-14 Prp19 WD40 domain
Beta-prop_Aladin PF25460 114 - 189 1.2e-08 Aladin seven-bladed propeller
Beta-prop_THOC3 PF25174 117 - 187 6.9e-16 THOC3 beta-propeller domain
WD40_Prp19 PF24814 129 - 236 2.2e-16 Prp19 WD40 domain
WD40 PF00400 150 - 186 5.5e-10 WD domain, G-beta repeat
EIF3I PF24805 154 - 238 6.7e-08 EIF3I
WD40 PF00400 197 - 236 4.3e-07 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 201 - 288 8.2e-09 WDR5 beta-propeller domain
WD40_WDHD1_1st PF24817 205 - 349 3.8e-08 WDHD1 first WD40 domain
Beta-prop_WDR3_1st PF25173 206 - 288 5.2e-06 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 208 - 288 2.2e-08 THOC3 beta-propeller domain
Beta-prop_EML_2 PF23414 212 - 346 7.9e-07 Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz PF24807 260 - 347 4.5e-06 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 261 - 348 4e-10 THOC3 beta-propeller domain
WD40 PF00400 309 - 342 2.3e-06 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 310 - 349 5e-06 WDR5 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 686
AccB7I CCANNNNNTGG 1 cut(s) 943
AciI CCGC 3 cut(s) 126, 396, 1001
AclWI GGATC 2 cut(s) 718, 987
AcoI YGGCCR 2 cut(s) 81, 255
AfiI CCNNNNNNNGG 5 cut(s) 86, 243, 737, 943, 976
AgsI TTSAA 1 cut(s) 596
AhdI GACNNNNNGTC 1 cut(s) 396
AjnI CCWGG 2 cut(s) 71, 192
AluBI AGCT 6 cut(s) 19, 267, 534, 839, 1030, 1044
AluI AGCT 6 cut(s) 19, 267, 534, 839, 1030, 1044
Alw21I GWGCWC 1 cut(s) 21
Alw26I GTCTC 1 cut(s) 657
AlwI GGATC 2 cut(s) 718, 987
Ama87I CYCGRG 2 cut(s) 434, 971
AoxI GGCC 5 cut(s) 43, 81, 255, 469, 899
ApeKI GCWGC 5 cut(s) 123, 430, 839, 998, 1044
Asp700I GAANNNNTTC 1 cut(s) 443
AspLEI GCGC 1 cut(s) 230
AspS9I GGNCC 3 cut(s) 43, 234, 899
AsuC2I CCSGG 4 cut(s) 435, 436, 972, 973
AsuHPI GGTGA 2 cut(s) 581, 617
AvaI CYCGRG 2 cut(s) 434, 971
AvaII GGWCC 1 cut(s) 234
BalI TGGCCA 1 cut(s) 257
BanII GRGCYC 3 cut(s) 21, 643, 971
Bbv12I GWGCWC 1 cut(s) 21
BbvI GCAGC 5 cut(s) 135, 417, 826, 1010, 1031
BccI CCATC 8 cut(s) 247, 379, 484, 560, 664, 731, 878, 890
BcgI CGANNNNNNTGC 2 cut(s) 744, 778
BciT130I CCWGG 2 cut(s) 73, 194
BcnI CCSGG 4 cut(s) 435, 436, 972, 973
BcoDI GTCTC 1 cut(s) 657
BfaI CTAG 2 cut(s) 329, 510
BfmI CTRYAG 5 cut(s) 38, 121, 679, 840, 996
BfuAI ACCTGC 1 cut(s) 686
BisI GCNGC 6 cut(s) 124, 127, 431, 840, 999, 1045
BlsI GCNGC 6 cut(s) 125, 128, 432, 841, 1000, 1046
Bme1390I CCNGG 6 cut(s) 73, 194, 435, 436, 972, 973
Bme18I GGWCC 1 cut(s) 234
BmeRI GACNNNNNGTC 1 cut(s) 396
BmeT110I CYCGRG 2 cut(s) 434, 971
BmgT120I GGNCC 3 cut(s) 43, 234, 899
BmiI GGNNCC 3 cut(s) 65, 236, 968
BmrFI CCNGG 6 cut(s) 73, 194, 435, 436, 972, 973
BmsI GCATC 4 cut(s) 262, 511, 717, 838
BpuMI CCSGG 4 cut(s) 435, 436, 972, 973
BsaJI CCNNGG 6 cut(s) 84, 434, 749, 970, 971, 972
BsaWI WCCGGW 1 cut(s) 786
BsaXI ACNNNNNCTCC 2 cut(s) 968, 998
Bsc4I CCNNNNNNNGG 5 cut(s) 86, 243, 737, 943, 976
Bse1I ACTGG 3 cut(s) 324, 657, 831
Bse3DI GCAATG 1 cut(s) 968
BseBI CCWGG 2 cut(s) 73, 194
BseDI CCNNGG 6 cut(s) 84, 434, 749, 970, 971, 972
BseGI GGATG 3 cut(s) 202, 502, 1009
BseLI CCNNNNNNNGG 5 cut(s) 86, 243, 737, 943, 976
BseMI GCAATG 1 cut(s) 968
BseNI ACTGG 3 cut(s) 324, 657, 831
BseXI GCAGC 5 cut(s) 135, 417, 826, 1010, 1031
Bsh1285I CGRYCG 2 cut(s) 224, 400
BshFI GGCC 5 cut(s) 45, 83, 257, 471, 901
BsiEI CGRYCG 2 cut(s) 224, 400
BsiHKAI GWGCWC 1 cut(s) 21
BsiHKCI CYCGRG 2 cut(s) 434, 971
BsiSI CCGG 4 cut(s) 80, 435, 787, 972
BslFI GGGAC 3 cut(s) 220, 830, 1024
BslI CCNNNNNNNGG 5 cut(s) 86, 243, 737, 943, 976
BsmAI GTCTC 1 cut(s) 657
BsmFI GGGAC 3 cut(s) 220, 830, 1024
BsnI GGCC 5 cut(s) 45, 83, 257, 471, 901
BsoBI CYCGRG 2 cut(s) 434, 971
Bsp1286I GDGCHC 3 cut(s) 21, 643, 971
Bsp143I GATC 4 cut(s) 221, 688, 710, 979
BspACI CCGC 3 cut(s) 126, 396, 1001
BspANI GGCC 5 cut(s) 45, 83, 257, 471, 901
BspHI TCATGA 2 cut(s) 337, 781
BspLI GGNNCC 3 cut(s) 65, 236, 968
BspMAI CTGCAG 4 cut(s) 125, 683, 844, 1000
BspMI ACCTGC 1 cut(s) 686
BspPI GGATC 2 cut(s) 718, 987
BsrDI GCAATG 1 cut(s) 968
BsrI ACTGG 3 cut(s) 324, 657, 831
BssECI CCNNGG 6 cut(s) 84, 434, 749, 970, 971, 972
BssMI GATC 4 cut(s) 221, 688, 710, 979
BssT1I CCWWGG 1 cut(s) 749
Bst2UI CCWGG 2 cut(s) 73, 194
Bst4CI ACNGT 5 cut(s) 139, 217, 452, 547, 634
Bst6I CTCTTC 1 cut(s) 631
BstAPI GCANNNNNTGC 1 cut(s) 848
BstC8I GCNNGC 2 cut(s) 265, 732
BstDSI CCRYGG 1 cut(s) 84
BstEII GGTNACC 1 cut(s) 673
BstF5I GGATG 3 cut(s) 202, 502, 1009
BstHHI GCGC 1 cut(s) 230
BstKTI GATC 4 cut(s) 224, 691, 713, 982
BstMAI GTCTC 1 cut(s) 657
BstMBI GATC 4 cut(s) 221, 688, 710, 979
BstMCI CGRYCG 2 cut(s) 224, 400
BstMWI GCNNNNNNNGC 4 cut(s) 281, 836, 848, 998
BstNI CCWGG 2 cut(s) 73, 194
BstNSI RCATGY 1 cut(s) 591
BstPI GGTNACC 1 cut(s) 673
BstSCI CCNGG 6 cut(s) 71, 192, 433, 434, 970, 971
BstSFI CTRYAG 5 cut(s) 38, 121, 679, 840, 996
BstV1I GCAGC 5 cut(s) 135, 417, 826, 1010, 1031
BsuRI GGCC 5 cut(s) 45, 83, 257, 471, 901
BtgI CCRYGG 1 cut(s) 84
BtsCI GGATG 3 cut(s) 202, 502, 1009
BtsI GCAGTG 2 cut(s) 258, 688
BtsIMutI CAGTG 7 cut(s) 48, 258, 503, 543, 664, 688, 838
BveI ACCTGC 1 cut(s) 686
Cac8I GCNNGC 2 cut(s) 265, 732
CciI TCATGA 2 cut(s) 337, 781
CfoI GCGC 1 cut(s) 230
Cfr13I GGNCC 3 cut(s) 43, 234, 899
Cfr9I CCCGGG 2 cut(s) 434, 971
CseI GACGC 1 cut(s) 43
CviAII CATG 5 cut(s) 338, 588, 782, 938, 943
DpnI GATC 4 cut(s) 223, 690, 712, 981
DpnII GATC 4 cut(s) 221, 688, 710, 979
DriI GACNNNNNGTC 1 cut(s) 396
EaeI YGGCCR 2 cut(s) 81, 255
Eam1104I CTCTTC 1 cut(s) 631
Eam1105I GACNNNNNGTC 1 cut(s) 396
EarI CTCTTC 1 cut(s) 631
Ecl136II GAGCTC 1 cut(s) 19
Eco130I CCWWGG 1 cut(s) 749
Eco24I GRGCYC 3 cut(s) 21, 643, 971
Eco32I GATATC 1 cut(s) 949
Eco47I GGWCC 1 cut(s) 234
Eco53kI GAGCTC 1 cut(s) 19
Eco88I CYCGRG 2 cut(s) 434, 971
Eco91I GGTNACC 1 cut(s) 673
EcoICRI GAGCTC 1 cut(s) 19
EcoO65I GGTNACC 1 cut(s) 673
EcoRII CCWGG 2 cut(s) 71, 192
EcoRV GATATC 1 cut(s) 949
EcoT14I CCWWGG 1 cut(s) 749
EcoT38I GRGCYC 3 cut(s) 21, 643, 971
ErhI CCWWGG 1 cut(s) 749
FaeI CATG 5 cut(s) 341, 591, 785, 941, 946
FaqI GGGAC 3 cut(s) 220, 830, 1024
FatI CATG 5 cut(s) 337, 587, 781, 937, 942
Fnu4HI GCNGC 6 cut(s) 124, 127, 431, 840, 999, 1045
FokI GGATG 3 cut(s) 209, 489, 1016
FriOI GRGCYC 3 cut(s) 21, 643, 971
Fsp4HI GCNGC 6 cut(s) 124, 127, 431, 840, 999, 1045
FspBI CTAG 2 cut(s) 329, 510
GlaI GCGC 1 cut(s) 229
GluI GCNGC 6 cut(s) 124, 127, 431, 840, 999, 1045
HaeIII GGCC 5 cut(s) 45, 83, 257, 471, 901
HapII CCGG 4 cut(s) 80, 435, 787, 972
HgaI GACGC 1 cut(s) 43
HhaI GCGC 1 cut(s) 230
Hin1II CATG 5 cut(s) 341, 591, 785, 941, 946
Hin6I GCGC 1 cut(s) 228
HinP1I GCGC 1 cut(s) 228
HindIII AAGCTT 1 cut(s) 265
HinfI GANTC 5 cut(s) 28, 76, 99, 367, 514
HpaII CCGG 4 cut(s) 80, 435, 787, 972
HphI GGTGA 2 cut(s) 581, 617
Hpy188I TCNGA 5 cut(s) 23, 33, 221, 715, 1039
Hpy188III TCNNGA 5 cut(s) 329, 338, 401, 550, 782
HpyAV CCTTC 4 cut(s) 7, 121, 912, 919
HpyCH4III ACNGT 5 cut(s) 139, 217, 452, 547, 634
HpyF10VI GCNNNNNNNGC 4 cut(s) 281, 836, 848, 998
Hsp92II CATG 5 cut(s) 341, 591, 785, 941, 946
HspAI GCGC 1 cut(s) 228
Kzo9I GATC 4 cut(s) 221, 688, 710, 979
LmnI GCTCC 6 cut(s) 16, 24, 63, 836, 966, 1027
Lsp1109I GCAGC 5 cut(s) 135, 417, 826, 1010, 1031
LweI GCATC 4 cut(s) 262, 511, 717, 838
MaeI CTAG 2 cut(s) 329, 510
MaeIII GTNAC 3 cut(s) 569, 620, 673
MalI GATC 4 cut(s) 223, 690, 712, 981
MboI GATC 4 cut(s) 221, 688, 710, 979
MboII GAAGA 3 cut(s) 162, 499, 618
MhlI GDGCHC 3 cut(s) 21, 643, 971
MlsI TGGCCA 1 cut(s) 257
MluCI AATT 3 cut(s) 285, 911, 952
MluNI TGGCCA 1 cut(s) 257
MmeI TCCRAC 1 cut(s) 344
MnlI CCTC 5 cut(s) 77, 172, 325, 915, 970
Mox20I TGGCCA 1 cut(s) 257
MroXI GAANNNNTTC 1 cut(s) 443
MscI TGGCCA 1 cut(s) 257
MseI TTAA 2 cut(s) 651, 1017
Msp20I TGGCCA 1 cut(s) 257
MspA1I CMGCKG 2 cut(s) 126, 1001
MspI CCGG 4 cut(s) 80, 435, 787, 972
MspR9I CCNGG 6 cut(s) 73, 194, 435, 436, 972, 973
MvaI CCWGG 2 cut(s) 73, 194
MwoI GCNNNNNNNGC 4 cut(s) 281, 836, 848, 998
NciI CCSGG 4 cut(s) 435, 436, 972, 973
NdeII GATC 4 cut(s) 221, 688, 710, 979
NlaIII CATG 5 cut(s) 341, 591, 785, 941, 946
NlaIV GGNNCC 3 cut(s) 65, 236, 968
NmuCI GTSAC 2 cut(s) 569, 620
NspI RCATGY 1 cut(s) 591
PagI TCATGA 2 cut(s) 337, 781
PdmI GAANNNNTTC 1 cut(s) 443
PfeI GAWTC 5 cut(s) 28, 76, 99, 367, 514
PflMI CCANNNNNTGG 1 cut(s) 943
PfoI TCCNGGA 1 cut(s) 192
PkrI GCNGC 6 cut(s) 125, 128, 432, 841, 1000, 1046
Ple19I CGATCG 1 cut(s) 224
Psp124BI GAGCTC 1 cut(s) 21
Psp6I CCWGG 2 cut(s) 71, 192
PspEI GGTNACC 1 cut(s) 673
PspGI CCWGG 2 cut(s) 71, 192
PspN4I GGNNCC 3 cut(s) 65, 236, 968
PspPI GGNCC 3 cut(s) 43, 234, 899
PstI CTGCAG 4 cut(s) 125, 683, 844, 1000
PvuI CGATCG 1 cut(s) 224
SacI GAGCTC 1 cut(s) 21
SaqAI TTAA 2 cut(s) 651, 1017
SatI GCNGC 6 cut(s) 124, 127, 431, 840, 999, 1045
Sau3AI GATC 4 cut(s) 221, 688, 710, 979
Sau96I GGNCC 3 cut(s) 43, 234, 899
ScrFI CCNGG 6 cut(s) 73, 194, 435, 436, 972, 973
SduI GDGCHC 3 cut(s) 21, 643, 971
SfaNI GCATC 4 cut(s) 262, 511, 717, 838
SfcI CTRYAG 5 cut(s) 38, 121, 679, 840, 996
SinI GGWCC 1 cut(s) 234
SmaI CCCGGG 2 cut(s) 436, 973
Sse9I AATT 3 cut(s) 285, 911, 952
SsiI CCGC 3 cut(s) 126, 396, 1001
SspI AATATT 1 cut(s) 795
SspMI CTAG 2 cut(s) 329, 510
SstI GAGCTC 1 cut(s) 21
StyD4I CCNGG 6 cut(s) 71, 192, 433, 434, 970, 971
StyI CCWWGG 1 cut(s) 749
TaaI ACNGT 5 cut(s) 139, 217, 452, 547, 634
TaqI TCGA 3 cut(s) 270, 400, 447
TasI AATT 3 cut(s) 285, 911, 952
TauI GCSGC 1 cut(s) 129
TfiI GAWTC 5 cut(s) 28, 76, 99, 367, 514
Tru1I TTAA 2 cut(s) 651, 1017
Tru9I TTAA 2 cut(s) 651, 1017
TscAI CASTG 7 cut(s) 55, 265, 503, 550, 664, 688, 838
TseFI GTSAC 2 cut(s) 569, 620
TseI GCWGC 5 cut(s) 123, 430, 839, 998, 1044
Tsp45I GTSAC 2 cut(s) 569, 620
TspDTI ATGAA 6 cut(s) 213, 327, 354, 794, 891, 894
TspMI CCCGGG 2 cut(s) 434, 971
TspRI CASTG 7 cut(s) 55, 265, 503, 550, 664, 688, 838
Van91I CCANNNNNTGG 1 cut(s) 943
VpaK11BI GGWCC 1 cut(s) 234
XbaI TCTAGA 1 cut(s) 328
XceI RCATGY 1 cut(s) 591
XmaI CCCGGG 2 cut(s) 434, 971
XmnI GAANNNNTTC 1 cut(s) 443
XspI CTAG 2 cut(s) 329, 510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.