AT4G32990

Essential component of the cytosolic iron-sulfur (Fe S) protein assembly machinery. Required for the maturation of extramitochondrial Fe S proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0007416
Physical Location & Seq
Forward (+)
15920230 .. 15923010
2781 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G32990.1

Sequence Viewer

Length: 987 bp
ATGAAAGTGATGGATAAGAATTTAGGGTTAGAGGAGGTTCAGAAACTTGAAGGTCACACTGATCGTGTTTGGAACGTCGCCTGGAATCCCGCCGCTGACGGTGTTATTGCTTCCTGCAGTGCCGATAAGACCGTACGAATCTGGGAGCAGAGTTCTCTTACTCGTTCCTGGACCTGCAAGCTTGGTCACCGTCTGGGAAGTTTCGATGGCAATACTTGCGTTTGGGAAAATTTTGCTACTGACTCCGAGTCTGTCTCTGTTTTACGGGGACATGAAAGCGAAGTCAAAAGTGTATCATGGAATGCATCTGGTTCGTTGCTTGCAACTTGTGGTAGAGACAAGTCTGTCTGGATTTGGGAAATACAACCAGAGGAGGACGATGAATTTGATACTATTGCAGTGTTAACTGGTCATTCAGAAGATGTTAAGATGGTCTTGTGGCATCCCACCATGGATGTTTTGTTCTCTTGCAGTTATGATAACACTATCAAGATTTGGTGCTCTGAGGATGAAGATGGTGATTATAATTGTGTACAAACCTTAAGTGAACTTAACAATGGTCATTCTTCTACCGTCTGGAGTATCTCTTTTAATGCTGCAGGGGACAAGATGGTCACTTGTAGTGATGATCTAGCAGTGAAAATATGGAAGACAGATATTTCAAGGATGCAATCTGGTGAAGGATATGTACCCTGGACTCATGTTTGTACACTCTCTGGTTTTCACGATCGTACCATATATTCAGTTCATTGGTCAAGGGACGGTGTTATTGCTAGTGGAGCAGGCGATGATACTATACAGTTATTTGTGGACAGCGACAGTGACTCTGTTGATGGACCTTCATACAAACTTTTGGTTAAGAAGGAGAAAGCACATGAAATGGATGTAAATTCGGTCCAATGGGCACCTGATAAGGAAAGCCGGTTGCTTGCCTCGGCCAGCGACGATAAAATGGTTAAGATTTGGAAACTTGCCTCGGAGCCATGA

Protein Analysis

328

Amino Acids

36.6

Weight (kDa)

4.7

Isoelectric Point (pI)

31.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_EIPR1 PF23609 7 - 48 3.7e-06 EIPR1 beta-propeller
WD40 PF00400 12 - 48 2.2e-08 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 12 - 59 1.1e-09 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 12 - 52 1.1e-06 WDR5 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 13 - 170 8e-10 TEP-1 second beta-propeller
WD40_Prp19 PF24814 13 - 53 8.2e-07 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 23 - 168 5.7e-08 Echinoderm microtubule-associated protein second beta-propeller
WD40_MABP1-WDR62_2nd PF24782 33 - 246 4.6e-07 MABP1/WDR62 second WD40 domain
WD40_CDC20-Fz PF24807 64 - 269 1.9e-14 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 65 - 217 2e-22 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 67 - 221 2.6e-24 WDR5 beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 78 - 167 8.5e-09 CAF1B/HIR1 beta-propeller domain
WD40 PF00400 83 - 119 4.8e-09 WD domain, G-beta repeat
Beta-prop_Aladin PF25460 83 - 169 7.3e-07 Aladin seven-bladed propeller
Beta-prop_WDR3_2nd PF25172 90 - 218 5.6e-13 WDR3 second beta-propeller domain
Beta-prop_EIPR1 PF23609 91 - 166 7.6e-06 EIPR1 beta-propeller
Beta-prop_THOC3 PF25174 91 - 170 1.5e-18 THOC3 beta-propeller domain
WD40_Prp19 PF24814 130 - 268 1.8e-13 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 130 - 266 1.1e-07 WDHD1 first WD40 domain
WD40 PF00400 131 - 166 7.2e-08 WD domain, G-beta repeat
EIF3I PF24805 134 - 219 1.6e-08 EIF3I
WD40 PF00400 177 - 216 1.4e-06 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 235 - 326 1.5e-06 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 240 - 324 9e-07 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 240 - 326 2e-10 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 242 - 325 4.1e-11 THOC3 beta-propeller domain
WD40_Prp19 PF24814 248 - 325 1.7e-06 Prp19 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 525
AasI GACNNNNNNGTC 2 cut(s) 344, 611
Acc36I ACCTGC 1 cut(s) 182
AccB1I GGYRCC 1 cut(s) 904
AciI CCGC 2 cut(s) 90, 93
AcoI YGGCCR 1 cut(s) 936
AcsI RAATTY 4 cut(s) 19, 229, 383, 889
AfaI GTAC 5 cut(s) 135, 534, 690, 709, 733
AflII CTTAAG 1 cut(s) 541
AgsI TTSAA 2 cut(s) 50, 663
AhdI GACNNNNNGTC 1 cut(s) 247
AjnI CCWGG 3 cut(s) 80, 167, 692
AloI GAACNNNNNNTCC 2 cut(s) 446, 478
AluBI AGCT 1 cut(s) 181
AluI AGCT 1 cut(s) 181
Alw21I GWGCWC 1 cut(s) 503
Alw26I GTCTC 2 cut(s) 259, 330
AoxI GGCC 1 cut(s) 936
ApeKI GCWGC 1 cut(s) 596
ApoI RAATTY 4 cut(s) 19, 229, 383, 889
ArsI GACNNNNNNTTYG 2 cut(s) 368, 400
AspS9I GGNCC 3 cut(s) 171, 836, 895
AsuHPI GGTGA 3 cut(s) 179, 530, 689
AvaII GGWCC 3 cut(s) 171, 836, 895
BaeGI GKGCMC 1 cut(s) 907
BaeI ACNNNNGTAYC 2 cut(s) 715, 748
BanI GGYRCC 1 cut(s) 904
BarI GAAGNNNNNNTAC 2 cut(s) 672, 704
BbsI GAAGAC 1 cut(s) 656
Bbv12I GWGCWC 1 cut(s) 503
BbvI GCAGC 1 cut(s) 583
BccI CCATC 6 cut(s) 4, 200, 424, 509, 604, 827
BcgI CGANNNNNNTGC 2 cut(s) 294, 328
BciT130I CCWGG 3 cut(s) 82, 169, 694
BcoDI GTCTC 2 cut(s) 259, 330
BfaI CTAG 2 cut(s) 632, 774
BfmI CTRYAG 2 cut(s) 115, 597
BfrI CTTAAG 1 cut(s) 541
BfuAI ACCTGC 1 cut(s) 182
BisI GCNGC 2 cut(s) 93, 597
BlsI GCNGC 2 cut(s) 94, 598
Bme1390I CCNGG 3 cut(s) 82, 169, 694
Bme18I GGWCC 3 cut(s) 171, 836, 895
BmeRI GACNNNNNGTC 1 cut(s) 247
BmgT120I GGNCC 3 cut(s) 171, 836, 895
BmiI GGNNCC 2 cut(s) 906, 981
BmrFI CCNGG 3 cut(s) 82, 169, 694
BmsI GCATC 3 cut(s) 314, 451, 657
BpiI GAAGAC 1 cut(s) 656
BplI GAGNNNNNCTC 2 cut(s) 239, 271
BpmI CTGGAG 1 cut(s) 598
BsaJI CCNNGG 4 cut(s) 450, 692, 933, 975
Bse118I RCCGGY 1 cut(s) 921
Bse1I ACTGG 1 cut(s) 412
BseBI CCWGG 3 cut(s) 82, 169, 694
BseDI CCNNGG 4 cut(s) 450, 692, 933, 975
BseGI GGATG 5 cut(s) 442, 460, 514, 672, 889
BseMII CTCAG 1 cut(s) 495
BseNI ACTGG 1 cut(s) 412
BseRI GAGGAG 2 cut(s) 47, 386
BseSI GKGCMC 1 cut(s) 907
BseXI GCAGC 1 cut(s) 583
Bsh1285I CGRYCG 1 cut(s) 730
BshFI GGCC 1 cut(s) 938
BshNI GGYRCC 1 cut(s) 904
BsiEI CGRYCG 1 cut(s) 730
BsiHKAI GWGCWC 1 cut(s) 503
BsiSI CCGG 1 cut(s) 922
BsiWI CGTACG 1 cut(s) 133
BslFI GGGAC 3 cut(s) 282, 617, 773
BsmAI GTCTC 2 cut(s) 259, 330
BsmFI GGGAC 3 cut(s) 282, 617, 773
BsmI GAATGC 1 cut(s) 307
BsnI GGCC 1 cut(s) 938
Bsp1286I GDGCHC 2 cut(s) 503, 907
Bsp1407I TGTACA 2 cut(s) 532, 707
Bsp143I GATC 3 cut(s) 61, 628, 727
Bsp19I CCATGG 1 cut(s) 450
BspACI CCGC 2 cut(s) 90, 93
BspANI GGCC 1 cut(s) 938
BspCNI CTCAG 1 cut(s) 496
BspLI GGNNCC 2 cut(s) 906, 981
BspMAI CTGCAG 2 cut(s) 119, 601
BspMI ACCTGC 1 cut(s) 182
BspT107I GGYRCC 1 cut(s) 904
BspTI CTTAAG 1 cut(s) 541
BsrFI RCCGGY 1 cut(s) 921
BsrGI TGTACA 2 cut(s) 532, 707
BsrI ACTGG 1 cut(s) 412
BssAI RCCGGY 1 cut(s) 921
BssECI CCNNGG 4 cut(s) 450, 692, 933, 975
BssMI GATC 3 cut(s) 61, 628, 727
BssT1I CCWWGG 1 cut(s) 450
Bst2UI CCWGG 3 cut(s) 82, 169, 694
Bst4CI ACNGT 7 cut(s) 101, 133, 191, 574, 764, 801, 821
BstAFI CTTAAG 1 cut(s) 541
BstAPI GCANNNNNTGC 1 cut(s) 216
BstAUI TGTACA 2 cut(s) 532, 707
BstC8I GCNNGC 5 cut(s) 179, 321, 784, 930, 940
BstDEI CTNAG 1 cut(s) 504
BstDSI CCRYGG 1 cut(s) 450
BstEII GGTNACC 1 cut(s) 185
BstF5I GGATG 5 cut(s) 442, 460, 514, 672, 889
BstKTI GATC 3 cut(s) 64, 631, 730
BstMAI GTCTC 2 cut(s) 259, 330
BstMBI GATC 3 cut(s) 61, 628, 727
BstMCI CGRYCG 1 cut(s) 730
BstMWI GCNNNNNNNGC 2 cut(s) 216, 779
BstNI CCWGG 3 cut(s) 82, 169, 694
BstPI GGTNACC 1 cut(s) 185
BstSCI CCNGG 3 cut(s) 80, 167, 692
BstSFI CTRYAG 2 cut(s) 115, 597
BstSLI GKGCMC 1 cut(s) 907
BstV1I GCAGC 1 cut(s) 583
BstV2I GAAGAC 1 cut(s) 656
BsuRI GGCC 1 cut(s) 938
BtgI CCRYGG 1 cut(s) 450
BtgZI GCGATG 1 cut(s) 801
BtsCI GGATG 5 cut(s) 442, 460, 514, 672, 889
BtsI GCAGTG 3 cut(s) 124, 405, 642
BtsIMutI CAGTG 5 cut(s) 57, 124, 405, 642, 826
BveI ACCTGC 1 cut(s) 182
Cac8I GCNNGC 5 cut(s) 179, 321, 784, 930, 940
Cfr10I RCCGGY 1 cut(s) 921
Cfr13I GGNCC 3 cut(s) 171, 836, 895
Csp6I GTAC 5 cut(s) 134, 533, 689, 708, 732
CviAII CATG 6 cut(s) 272, 297, 451, 701, 875, 984
CviJI RGCY 4 cut(s) 181, 921, 938, 982
CviKI_1 RGCY 4 cut(s) 181, 921, 938, 982
CviQI GTAC 5 cut(s) 134, 533, 689, 708, 732
DdeI CTNAG 1 cut(s) 504
DpnI GATC 3 cut(s) 63, 630, 729
DpnII GATC 3 cut(s) 61, 628, 727
DrdI GACNNNNNNGTC 2 cut(s) 344, 611
DriI GACNNNNNGTC 1 cut(s) 247
DseDI GACNNNNNNGTC 2 cut(s) 344, 611
EaeI YGGCCR 1 cut(s) 936
Eam1105I GACNNNNNGTC 1 cut(s) 247
Eco130I CCWWGG 1 cut(s) 450
Eco47I GGWCC 3 cut(s) 171, 836, 895
Eco91I GGTNACC 1 cut(s) 185
EcoO65I GGTNACC 1 cut(s) 185
EcoRII CCWGG 3 cut(s) 80, 167, 692
EcoT14I CCWWGG 1 cut(s) 450
EcoT22I ATGCAT 1 cut(s) 307
ErhI CCWWGG 1 cut(s) 450
FaeI CATG 6 cut(s) 275, 300, 454, 704, 878, 987
FalI AAGNNNNNCTT 2 cut(s) 419, 451
FaqI GGGAC 3 cut(s) 282, 617, 773
FatI CATG 6 cut(s) 271, 296, 450, 700, 874, 983
FauI CCCGC 1 cut(s) 97
Fnu4HI GCNGC 2 cut(s) 93, 597
FokI GGATG 5 cut(s) 429, 467, 521, 679, 896
Fsp4HI GCNGC 2 cut(s) 93, 597
FspBI CTAG 2 cut(s) 632, 774
GluI GCNGC 2 cut(s) 93, 597
GsuI CTGGAG 1 cut(s) 598
HaeIII GGCC 1 cut(s) 938
HapII CCGG 1 cut(s) 922
Hin1II CATG 6 cut(s) 275, 300, 454, 704, 878, 987
HincII GTYRAC 1 cut(s) 405
HindII GTYRAC 1 cut(s) 405
HindIII AAGCTT 1 cut(s) 179
HinfI GANTC 6 cut(s) 85, 138, 242, 248, 697, 824
HpaI GTTAAC 1 cut(s) 405
HpaII CCGG 1 cut(s) 922
HphI GGTGA 3 cut(s) 179, 530, 689
Hpy166II GTNNAC 5 cut(s) 405, 533, 548, 710, 811
Hpy188I TCNGA 5 cut(s) 42, 247, 418, 505, 979
Hpy188III TCNNGA 4 cut(s) 349, 490, 577, 725
Hpy8I GTNNAC 5 cut(s) 405, 533, 548, 710, 811
Hpy99I CGWCG 2 cut(s) 80, 947
HpyAV CCTTC 4 cut(s) 44, 674, 849, 856
HpyCH4III ACNGT 7 cut(s) 101, 133, 191, 574, 764, 801, 821
HpyCH4IV ACGT 1 cut(s) 75
HpyCH4V TGCA 8 cut(s) 117, 177, 305, 323, 398, 471, 599, 670
HpyF10VI GCNNNNNNNGC 2 cut(s) 216, 779
HpyF3I CTNAG 1 cut(s) 504
HpySE526I ACGT 1 cut(s) 75
Hsp92II CATG 6 cut(s) 275, 300, 454, 704, 878, 987
KspAI GTTAAC 1 cut(s) 405
Kzo9I GATC 3 cut(s) 61, 628, 727
LmnI GCTCC 3 cut(s) 145, 779, 979
Lsp1109I GCAGC 1 cut(s) 583
LweI GCATC 3 cut(s) 314, 451, 657
MaeI CTAG 2 cut(s) 632, 774
MaeII ACGT 1 cut(s) 75
MaeIII GTNAC 4 cut(s) 53, 185, 613, 821
MalI GATC 3 cut(s) 63, 630, 729
MboI GATC 3 cut(s) 61, 628, 727
MboII GAAGA 4 cut(s) 431, 524, 558, 661
MhlI GDGCHC 2 cut(s) 503, 907
MluCI AATT 5 cut(s) 19, 229, 383, 526, 889
MlyI GAGTC 4 cut(s) 236, 257, 691, 818
MnlI CCTC 7 cut(s) 25, 28, 364, 367, 499, 943, 985
Mph1103I ATGCAT 1 cut(s) 307
MseI TTAA 7 cut(s) 404, 426, 542, 552, 591, 858, 957
MspA1I CMGCKG 1 cut(s) 95
MspCI CTTAAG 1 cut(s) 541
MspI CCGG 1 cut(s) 922
MspR9I CCNGG 3 cut(s) 82, 169, 694
Mva1269I GAATGC 1 cut(s) 307
MvaI CCWGG 3 cut(s) 82, 169, 694
MwoI GCNNNNNNNGC 2 cut(s) 216, 779
NcoI CCATGG 1 cut(s) 450
NdeII GATC 3 cut(s) 61, 628, 727
NlaIII CATG 6 cut(s) 275, 300, 454, 704, 878, 987
NlaIV GGNNCC 2 cut(s) 906, 981
NmeAIII GCCGAG 1 cut(s) 914
NmuCI GTSAC 4 cut(s) 53, 185, 613, 821
NsiI ATGCAT 1 cut(s) 307
PctI GAATGC 1 cut(s) 307
PfeI GAWTC 2 cut(s) 85, 138
Pfl23II CGTACG 1 cut(s) 133
PfoI TCCNGGA 1 cut(s) 167
PkrI GCNGC 2 cut(s) 94, 598
Ple19I CGATCG 1 cut(s) 730
PleI GAGTC 4 cut(s) 236, 256, 691, 818
PpsI GAGTC 4 cut(s) 236, 256, 691, 818
PsiI TTATAA 1 cut(s) 525
Psp6I CCWGG 3 cut(s) 80, 167, 692
PspEI GGTNACC 1 cut(s) 185
PspGI CCWGG 3 cut(s) 80, 167, 692
PspLI CGTACG 1 cut(s) 133
PspN4I GGNNCC 2 cut(s) 906, 981
PspPI GGNCC 3 cut(s) 171, 836, 895
PstI CTGCAG 2 cut(s) 119, 601
PvuI CGATCG 1 cut(s) 730
RsaI GTAC 5 cut(s) 135, 534, 690, 709, 733
RsaNI GTAC 5 cut(s) 134, 533, 689, 708, 732
SaqAI TTAA 7 cut(s) 404, 426, 542, 552, 591, 858, 957
SatI GCNGC 2 cut(s) 93, 597
Sau3AI GATC 3 cut(s) 61, 628, 727
Sau96I GGNCC 3 cut(s) 171, 836, 895
SchI GAGTC 4 cut(s) 236, 257, 691, 818
ScrFI CCNGG 3 cut(s) 82, 169, 694
SduI GDGCHC 2 cut(s) 503, 907
SetI ASST 8 cut(s) 39, 55, 78, 176, 183, 542, 841, 910
SfaNI GCATC 3 cut(s) 314, 451, 657
SfcI CTRYAG 2 cut(s) 115, 597
SinI GGWCC 3 cut(s) 171, 836, 895
SmlI CTYRAG 1 cut(s) 541
SmoI CTYRAG 1 cut(s) 541
Sse9I AATT 5 cut(s) 19, 229, 383, 526, 889
SsiI CCGC 2 cut(s) 90, 93
SspMI CTAG 2 cut(s) 632, 774
StyD4I CCNGG 3 cut(s) 80, 167, 692
StyI CCWWGG 1 cut(s) 450
TaaI ACNGT 7 cut(s) 101, 133, 191, 574, 764, 801, 821
TaiI ACGT 1 cut(s) 78
TaqI TCGA 1 cut(s) 204
TaqII GACCGA 1 cut(s) 883
TasI AATT 5 cut(s) 19, 229, 383, 526, 889
TatI WGTACW 2 cut(s) 532, 707
TauI GCSGC 1 cut(s) 95
TfiI GAWTC 2 cut(s) 85, 138
Tru1I TTAA 7 cut(s) 404, 426, 542, 552, 591, 858, 957
Tru9I TTAA 7 cut(s) 404, 426, 542, 552, 591, 858, 957
TscAI CASTG 5 cut(s) 64, 124, 405, 642, 826
TseFI GTSAC 4 cut(s) 53, 185, 613, 821
TseI GCWGC 1 cut(s) 596
Tsp45I GTSAC 4 cut(s) 53, 185, 613, 821
TspDTI ATGAA 7 cut(s) 17, 288, 396, 525, 737, 831, 891
TspRI CASTG 5 cut(s) 64, 124, 405, 642, 826
Vha464I CTTAAG 1 cut(s) 541
VpaK11BI GGWCC 3 cut(s) 171, 836, 895
XapI RAATTY 4 cut(s) 19, 229, 383, 889
XspI CTAG 2 cut(s) 632, 774
Zsp2I ATGCAT 1 cut(s) 307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.