Rroxscaffold_6G00408020

Essential component of the cytosolic iron-sulfur (Fe S) protein assembly machinery. Required for the maturation of extramitochondrial Fe S proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0007416
Physical Location & Seq
Forward (+)
30540843 .. 30544946
4104 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00408020.1

Sequence Viewer

Length: 999 bp
ATGGAGGGTCTCAAGGAGATTCAGAAGCTGGAGGGCCACACTGATAGGGTGTGGAGCCTCGCGTGGAACCCACTCTCCGGTCTCGACGGAGCCCCCTTAAGCTCGCTTCATGCAGCGGCGATAAGACCGTTCGCAGTTCTTGATGATCCGCACACCAGGACCGTCCGATCGTGCGCTTGGTCGCCGTCCGGGAAGCTACTGGCCACTGCCAGCTTCGATGGCACCACCGCAATTTTTCAGGACGTTGGGGATGATTATGAGTGCGTTTCGACTCTAGAGGGTCATGAAAATGAAGTGAAGAGTGTGTCTTGGAATGCGTCTGGGTCTTTGCTTGCGACTTGTGGGAGAGATAAGACTGTGTGGATATGGGAGGTGTTACCCGGCAATGAGTTTGAGTGCGTTGCGGTGTTGCAAGGCCATACACAAGATGTGAAAATGGTTCAGTGGCATCCGACTATGGAGCTTTTGTTTTCGTGTAGCTATGACAACACTGTCAAGATATGGGCGGATGATGGTGAGGATGATAATTGGGTGTGTGTGCAAACGTTAGCTCAATCTAACAGTGGTCACTCTTCTACTGTATGGGCTCTATCGTTTAATGCTACTGGAGATAAAATGGTTACCTGCAGTGATGATCTTACCTTAAAGATATGGGGAACAGACAATGGGAAGATGCTATCTTCTGATGGCTTTGCACCTTGGAGTCATCTTTGTACTGTTGCGGGTTATCATGATAGGACAATATTTTCAGTTCATTGGTCAAGGGACAATATCATTGCCACTGGAGCTGCTGATGATGCCATAAGATTCTTTGTGGAGAATAATGAGTGTGATACTTTGGTTGATGGGCCTTCCTATAATTTGCTTCTGAAGAAGGAAAAGGCCCATGACATGGATATAAATTCGGTACAGTGGAGCCCTGGGGAGAATAGGCTTCTTGCTTCTGCAGCTGATGATGGGACGATTAAAATATGGGAGCTGACATCTGTAAGCTGCTGA

Protein Analysis

332

Amino Acids

36.51

Weight (kDa)

4.61

Isoelectric Point (pI)

26.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 5 - 127 6.3e-12 CDC20/Fizzy WD40 domain
WD40 PF00400 50 - 78 4e-06 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 50 - 218 1.4e-27 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 50 - 227 7.5e-36 WDR5 beta-propeller domain
WD40_Prp19 PF24814 50 - 129 6.9e-11 Prp19 WD40 domain
Beta-prop_EML PF23409 51 - 268 1.1e-07 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_THOC3 PF25174 51 - 104 8.1e-09 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 52 - 228 1.7e-19 CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_1st PF25171 53 - 169 3.8e-06 WDR36/Utp21 first beta-propeller
Beta-prop_EIPR1 PF23609 53 - 168 3.9e-06 EIPR1 beta-propeller
WD40_MABP1-WDR62_2nd PF24782 53 - 225 6.5e-11 MABP1/WDR62 second WD40 domain
Beta-prop_EML_2 PF23414 53 - 169 4.3e-12 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_2nd PF25172 54 - 220 2e-20 WDR3 second beta-propeller domain
WD40_Gbeta PF25391 55 - 219 7.7e-10 G protein beta WD-40 repeat protein
Beta-prop_TEP1_2nd PF25047 83 - 220 6.1e-12 TEP-1 second beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 86 - 168 1.5e-08 CAF1B/HIR1 beta-propeller domain
WD40 PF00400 87 - 123 1.2e-10 WD domain, G-beta repeat
Beta-prop_Aladin PF25460 92 - 199 4.1e-08 Aladin seven-bladed propeller
Beta-prop_THOC3 PF25174 95 - 169 2.3e-19 THOC3 beta-propeller domain
WD40_Prp19 PF24814 107 - 227 3.2e-19 Prp19 WD40 domain
Beta-prop_CAF1B_HIR1 PF24105 111 - 227 8e-10 CAF1B/HIR1 beta-propeller domain
WD40_WDHD1_1st PF24817 130 - 191 7.1e-06 WDHD1 first WD40 domain
WD40 PF00400 132 - 168 9.9e-10 WD domain, G-beta repeat
EIF3I PF24805 136 - 328 8.2e-11 EIF3I
WDR55 PF24796 142 - 269 6.7e-06 WDR55
WD40 PF00400 179 - 218 5.5e-07 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 183 - 271 9.8e-08 WDR5 beta-propeller domain
WD40_WDHD1_1st PF24817 187 - 330 1.6e-07 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 190 - 273 4.5e-09 THOC3 beta-propeller domain
Beta-prop_EML_2 PF23414 194 - 328 1.3e-07 Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz PF24807 222 - 329 1.3e-06 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 244 - 330 5.2e-10 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 244 - 331 4.5e-08 WDR5 beta-propeller domain
WD40_Prp19 PF24814 249 - 329 7.7e-06 Prp19 WD40 domain
WD40 PF00400 293 - 325 7.6e-07 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 491
Acc36I ACCTGC 1 cut(s) 632
AccB1I GGYRCC 1 cut(s) 221
AccB7I CCANNNNNTGG 1 cut(s) 892
AccII CGCG 1 cut(s) 62
AciI CCGC 6 cut(s) 116, 149, 228, 404, 506, 722
AclI AACGTT 1 cut(s) 545
AclWI GGATC 1 cut(s) 140
AcoI YGGCCR 1 cut(s) 201
AcsI RAATTY 1 cut(s) 901
AcuI CTGAAG 1 cut(s) 890
AfaI GTAC 2 cut(s) 715, 909
AfiI CCNNNNNNNGG 2 cut(s) 77, 892
AflII CTTAAG 1 cut(s) 97
AjnI CCWGG 2 cut(s) 155, 919
AloI GAACNNNNNNTCC 2 cut(s) 59, 91
Alw26I GTCTC 2 cut(s) 14, 86
AlwI GGATC 1 cut(s) 140
AlwNI CAGNNNCTG 1 cut(s) 28
AoxI GGCC 5 cut(s) 34, 201, 415, 848, 882
ApeKI GCWGC 4 cut(s) 113, 788, 947, 993
ApoI RAATTY 1 cut(s) 901
AspLEI GCGC 1 cut(s) 176
AspS9I GGNCC 4 cut(s) 34, 159, 848, 883
AsuC2I CCSGG 2 cut(s) 190, 381
AsuHPI GGTGA 1 cut(s) 527
AvaII GGWCC 1 cut(s) 159
BaeI ACNNNNGTAYC 2 cut(s) 825, 858
BalI TGGCCA 1 cut(s) 203
BanI GGYRCC 1 cut(s) 221
BanII GRGCYC 3 cut(s) 94, 589, 920
BbvI GCAGC 4 cut(s) 125, 775, 959, 980
BccI CCATC 5 cut(s) 212, 506, 680, 839, 950
BceAI ACGGC 1 cut(s) 169
BciT130I CCWGG 2 cut(s) 157, 921
BcnI CCSGG 2 cut(s) 190, 381
BcoDI GTCTC 2 cut(s) 14, 86
BfaI CTAG 1 cut(s) 275
BfmI CTRYAG 2 cut(s) 625, 945
BfrI CTTAAG 1 cut(s) 97
BfuAI ACCTGC 1 cut(s) 632
BisI GCNGC 5 cut(s) 114, 117, 789, 948, 994
BlsI GCNGC 5 cut(s) 115, 118, 790, 949, 995
Bme1390I CCNGG 4 cut(s) 157, 190, 381, 921
Bme18I GGWCC 1 cut(s) 159
BmgT120I GGNCC 4 cut(s) 34, 159, 848, 883
BmiI GGNNCC 5 cut(s) 56, 68, 91, 223, 917
BmrFI CCNGG 4 cut(s) 157, 190, 381, 921
BmsI GCATC 3 cut(s) 457, 663, 787
BpmI CTGGAG 3 cut(s) 50, 627, 804
BpuMI CCSGG 2 cut(s) 190, 381
BsaI GGTCTC 2 cut(s) 14, 86
BsaJI CCNNGG 3 cut(s) 698, 919, 920
BsaWI WCCGGW 1 cut(s) 77
BsaXI ACNNNNNCTCC 4 cut(s) 59, 89, 452, 482
Bsc4I CCNNNNNNNGG 2 cut(s) 77, 892
Bse1I ACTGG 3 cut(s) 204, 610, 787
Bse3DI GCAATG 2 cut(s) 391, 774
BseBI CCWGG 2 cut(s) 157, 921
BseDI CCNNGG 3 cut(s) 698, 919, 920
BseGI GGATG 4 cut(s) 256, 448, 514, 526
BseLI CCNNNNNNNGG 2 cut(s) 77, 892
BseMI GCAATG 2 cut(s) 391, 774
BseNI ACTGG 3 cut(s) 204, 610, 787
BseXI GCAGC 4 cut(s) 125, 775, 959, 980
Bsh1236I CGCG 1 cut(s) 62
Bsh1285I CGRYCG 1 cut(s) 170
BshFI GGCC 5 cut(s) 36, 203, 417, 850, 884
BshNI GGYRCC 1 cut(s) 221
BsiEI CGRYCG 1 cut(s) 170
BsiSI CCGG 3 cut(s) 78, 189, 381
BslFI GGGAC 2 cut(s) 779, 973
BslI CCNNNNNNNGG 2 cut(s) 77, 892
BsmAI GTCTC 2 cut(s) 14, 86
BsmFI GGGAC 2 cut(s) 779, 973
BsmI GAATGC 1 cut(s) 319
BsnI GGCC 5 cut(s) 36, 203, 417, 850, 884
Bso31I GGTCTC 2 cut(s) 14, 86
Bsp1286I GDGCHC 3 cut(s) 94, 589, 920
Bsp143I GATC 3 cut(s) 145, 167, 634
BspACI CCGC 6 cut(s) 116, 149, 228, 404, 506, 722
BspANI GGCC 5 cut(s) 36, 203, 417, 850, 884
BspFNI CGCG 1 cut(s) 62
BspHI TCATGA 2 cut(s) 283, 730
BspLI GGNNCC 5 cut(s) 56, 68, 91, 223, 917
BspMAI CTGCAG 2 cut(s) 629, 949
BspMI ACCTGC 1 cut(s) 632
BspPI GGATC 1 cut(s) 140
BspT107I GGYRCC 1 cut(s) 221
BspTI CTTAAG 1 cut(s) 97
BspTNI GGTCTC 2 cut(s) 14, 86
BsrDI GCAATG 2 cut(s) 391, 774
BsrI ACTGG 3 cut(s) 204, 610, 787
BssECI CCNNGG 3 cut(s) 698, 919, 920
BssMI GATC 3 cut(s) 145, 167, 634
BssT1I CCWWGG 1 cut(s) 698
Bst2UI CCWGG 2 cut(s) 157, 921
Bst4CI ACNGT 8 cut(s) 129, 163, 358, 493, 563, 580, 718, 912
Bst6I CTCTTC 2 cut(s) 293, 577
BstAFI CTTAAG 1 cut(s) 97
BstC8I GCNNGC 3 cut(s) 104, 211, 333
BstEII GGTNACC 1 cut(s) 619
BstF5I GGATG 4 cut(s) 256, 448, 514, 526
BstFNI CGCG 1 cut(s) 62
BstHHI GCGC 1 cut(s) 176
BstKTI GATC 3 cut(s) 148, 170, 637
BstMAI GTCTC 2 cut(s) 14, 86
BstMBI GATC 3 cut(s) 145, 167, 634
BstMCI CGRYCG 1 cut(s) 170
BstMWI GCNNNNNNNGC 4 cut(s) 219, 785, 797, 947
BstNI CCWGG 2 cut(s) 157, 921
BstPI GGTNACC 1 cut(s) 619
BstSCI CCNGG 4 cut(s) 155, 188, 379, 919
BstSFI CTRYAG 2 cut(s) 625, 945
BstUI CGCG 1 cut(s) 62
BstV1I GCAGC 4 cut(s) 125, 775, 959, 980
BsuRI GGCC 5 cut(s) 36, 203, 417, 850, 884
BtsCI GGATG 4 cut(s) 256, 448, 514, 526
BtsI GCAGTG 2 cut(s) 204, 634
BtsIMutI CAGTG 8 cut(s) 39, 204, 449, 489, 568, 634, 780, 917
BveI ACCTGC 1 cut(s) 632
Cac8I GCNNGC 3 cut(s) 104, 211, 333
CaiI CAGNNNCTG 1 cut(s) 28
CciI TCATGA 2 cut(s) 283, 730
CfoI GCGC 1 cut(s) 176
Cfr13I GGNCC 4 cut(s) 34, 159, 848, 883
CseI GACGC 1 cut(s) 306
Csp6I GTAC 2 cut(s) 714, 908
CviAII CATG 5 cut(s) 110, 284, 731, 887, 892
CviQI GTAC 2 cut(s) 714, 908
DpnI GATC 3 cut(s) 147, 169, 636
DpnII GATC 3 cut(s) 145, 167, 634
DrdI GACNNNNNNGTC 1 cut(s) 491
DseDI GACNNNNNNGTC 1 cut(s) 491
EaeI YGGCCR 1 cut(s) 201
Eam1104I CTCTTC 2 cut(s) 293, 577
EarI CTCTTC 2 cut(s) 293, 577
EciI GGCGGA 1 cut(s) 521
Eco130I CCWWGG 1 cut(s) 698
Eco24I GRGCYC 3 cut(s) 94, 589, 920
Eco31I GGTCTC 2 cut(s) 14, 86
Eco47I GGWCC 1 cut(s) 159
Eco57I CTGAAG 1 cut(s) 890
Eco91I GGTNACC 1 cut(s) 619
EcoO65I GGTNACC 1 cut(s) 619
EcoRII CCWGG 2 cut(s) 155, 919
EcoT14I CCWWGG 1 cut(s) 698
EcoT38I GRGCYC 3 cut(s) 94, 589, 920
ErhI CCWWGG 1 cut(s) 698
FaeI CATG 5 cut(s) 113, 287, 734, 890, 895
FaqI GGGAC 2 cut(s) 779, 973
FatI CATG 5 cut(s) 109, 283, 730, 886, 891
FauI CCCGC 1 cut(s) 715
Fnu4HI GCNGC 5 cut(s) 114, 117, 789, 948, 994
FokI GGATG 4 cut(s) 263, 435, 521, 533
FriOI GRGCYC 3 cut(s) 94, 589, 920
Fsp4HI GCNGC 5 cut(s) 114, 117, 789, 948, 994
FspBI CTAG 1 cut(s) 275
GlaI GCGC 1 cut(s) 175
GluI GCNGC 5 cut(s) 114, 117, 789, 948, 994
GsuI CTGGAG 3 cut(s) 50, 627, 804
HaeIII GGCC 5 cut(s) 36, 203, 417, 850, 884
HapII CCGG 3 cut(s) 78, 189, 381
HgaI GACGC 1 cut(s) 306
HhaI GCGC 1 cut(s) 176
Hin1II CATG 5 cut(s) 113, 287, 734, 890, 895
Hin6I GCGC 1 cut(s) 174
HinP1I GCGC 1 cut(s) 174
HinfI GANTC 4 cut(s) 19, 271, 703, 807
HpaII CCGG 3 cut(s) 78, 189, 381
HphI GGTGA 1 cut(s) 527
Hpy188I TCNGA 5 cut(s) 24, 167, 453, 685, 870
Hpy188III TCNNGA 7 cut(s) 83, 140, 239, 275, 284, 496, 731
Hpy99I CGWCG 1 cut(s) 89
HpyAV CCTTC 2 cut(s) 861, 868
HpyCH4III ACNGT 8 cut(s) 129, 163, 358, 493, 563, 580, 718, 912
HpyCH4IV ACGT 2 cut(s) 243, 545
HpyCH4V TGCA 6 cut(s) 113, 412, 541, 627, 695, 947
HpyF10VI GCNNNNNNNGC 4 cut(s) 219, 785, 797, 947
HpySE526I ACGT 2 cut(s) 243, 545
Hsp92II CATG 5 cut(s) 113, 287, 734, 890, 895
HspAI GCGC 1 cut(s) 174
Kzo9I GATC 3 cut(s) 145, 167, 634
LmnI GCTCC 6 cut(s) 54, 89, 460, 785, 915, 976
Lsp1109I GCAGC 4 cut(s) 125, 775, 959, 980
LweI GCATC 3 cut(s) 457, 663, 787
MaeI CTAG 1 cut(s) 275
MaeII ACGT 2 cut(s) 243, 545
MaeIII GTNAC 3 cut(s) 375, 566, 619
MalI GATC 3 cut(s) 147, 169, 636
MboI GATC 3 cut(s) 145, 167, 634
MboII GAAGA 5 cut(s) 310, 564, 672, 682, 883
MhlI GDGCHC 3 cut(s) 94, 589, 920
MlsI TGGCCA 1 cut(s) 203
MluCI AATT 4 cut(s) 231, 526, 859, 901
MluNI TGGCCA 1 cut(s) 203
MlyI GAGTC 2 cut(s) 265, 712
MmeI TCCRAC 1 cut(s) 476
MnlI CCTC 5 cut(s) 25, 68, 271, 364, 511
Mox20I TGGCCA 1 cut(s) 203
MscI TGGCCA 1 cut(s) 203
MseI TTAA 4 cut(s) 98, 597, 644, 966
MslI CAYNNNNRTG 1 cut(s) 288
Msp20I TGGCCA 1 cut(s) 203
MspA1I CMGCKG 2 cut(s) 116, 950
MspCI CTTAAG 1 cut(s) 97
MspI CCGG 3 cut(s) 78, 189, 381
MspR9I CCNGG 4 cut(s) 157, 190, 381, 921
Mva1269I GAATGC 1 cut(s) 319
MvaI CCWGG 2 cut(s) 157, 921
MvnI CGCG 1 cut(s) 62
MwoI GCNNNNNNNGC 4 cut(s) 219, 785, 797, 947
NciI CCSGG 2 cut(s) 190, 381
NdeII GATC 3 cut(s) 145, 167, 634
NlaIII CATG 5 cut(s) 113, 287, 734, 890, 895
NlaIV GGNNCC 5 cut(s) 56, 68, 91, 223, 917
NmuCI GTSAC 1 cut(s) 566
PagI TCATGA 2 cut(s) 283, 730
PasI CCCWGGG 1 cut(s) 920
PctI GAATGC 1 cut(s) 319
PfeI GAWTC 2 cut(s) 19, 807
PflMI CCANNNNNTGG 1 cut(s) 892
PfoI TCCNGGA 1 cut(s) 188
PkrI GCNGC 5 cut(s) 115, 118, 790, 949, 995
Ple19I CGATCG 1 cut(s) 170
PleI GAGTC 2 cut(s) 265, 711
PpsI GAGTC 2 cut(s) 265, 711
Psp1406I AACGTT 1 cut(s) 545
Psp6I CCWGG 2 cut(s) 155, 919
PspEI GGTNACC 1 cut(s) 619
PspGI CCWGG 2 cut(s) 155, 919
PspN4I GGNNCC 5 cut(s) 56, 68, 91, 223, 917
PspPI GGNCC 4 cut(s) 34, 159, 848, 883
PstI CTGCAG 2 cut(s) 629, 949
PstNI CAGNNNCTG 1 cut(s) 28
PvuI CGATCG 1 cut(s) 170
PvuII CAGCTG 1 cut(s) 950
RsaI GTAC 2 cut(s) 715, 909
RsaNI GTAC 2 cut(s) 714, 908
RseI CAYNNNNRTG 1 cut(s) 288
SaqAI TTAA 4 cut(s) 98, 597, 644, 966
SatI GCNGC 5 cut(s) 114, 117, 789, 948, 994
Sau3AI GATC 3 cut(s) 145, 167, 634
Sau96I GGNCC 4 cut(s) 34, 159, 848, 883
SchI GAGTC 2 cut(s) 265, 712
ScrFI CCNGG 4 cut(s) 157, 190, 381, 921
SduI GDGCHC 3 cut(s) 94, 589, 920
SfaNI GCATC 3 cut(s) 457, 663, 787
SfcI CTRYAG 2 cut(s) 625, 945
SinI GGWCC 1 cut(s) 159
SmiMI CAYNNNNRTG 1 cut(s) 288
SmlI CTYRAG 2 cut(s) 11, 97
SmoI CTYRAG 2 cut(s) 11, 97
Sse9I AATT 4 cut(s) 231, 526, 859, 901
SsiI CCGC 6 cut(s) 116, 149, 228, 404, 506, 722
SspI AATATT 1 cut(s) 744
SspMI CTAG 1 cut(s) 275
StyD4I CCNGG 4 cut(s) 155, 188, 379, 919
StyI CCWWGG 1 cut(s) 698
TaaI ACNGT 8 cut(s) 129, 163, 358, 493, 563, 580, 718, 912
TaiI ACGT 2 cut(s) 246, 548
TaqI TCGA 3 cut(s) 84, 216, 269
TasI AATT 4 cut(s) 231, 526, 859, 901
TatI WGTACW 1 cut(s) 713
TauI GCSGC 1 cut(s) 119
TfiI GAWTC 2 cut(s) 19, 807
Tru1I TTAA 4 cut(s) 98, 597, 644, 966
Tru9I TTAA 4 cut(s) 98, 597, 644, 966
TscAI CASTG 8 cut(s) 46, 211, 449, 496, 568, 634, 787, 917
TseFI GTSAC 1 cut(s) 566
TseI GCWGC 4 cut(s) 113, 788, 947, 993
Tsp45I GTSAC 1 cut(s) 566
TspDTI ATGAA 4 cut(s) 98, 300, 306, 743
TspGWI ACGGA 1 cut(s) 102
TspRI CASTG 8 cut(s) 46, 211, 449, 496, 568, 634, 787, 917
Van91I CCANNNNNTGG 1 cut(s) 892
Vha464I CTTAAG 1 cut(s) 97
VpaK11BI GGWCC 1 cut(s) 159
XapI RAATTY 1 cut(s) 901
XbaI TCTAGA 1 cut(s) 274
XspI CTAG 1 cut(s) 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.