AT2G31110

PMR5 N terminal Domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0007940
Physical Location & Seq
Reverse (-)
13258329 .. 13262256
3928 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G31110.1

Sequence Viewer

Length: 651 bp
ATGACTGTAAAACCAAAAGAAAAGGATTATGGAGTGACGATAAATTTGTATAGAACACAGTTTCTAGTAGATGTGGTTCAAGAAAAGGCAGGACGAGTGCTTGTGCTTGACTCCATCAAACAAGCCGATGCTTGGCTTGGCATGGATGTTCTTATCTTCAACTCGTGGCACTGGTGGACTCACACCTCCGGACTTCAGCCCTGGGATTATATGAGGGAAGGAAACCAATTGTACAAAGACATGAACAGGCTTGTGGCTTATTACAAAGGACTTAACACATGGGCTCGTTGGATTAACAATAACATTGTCCCTTCACGTACTCAAGTCTTCTTTCAAGGTGTTTCTCCTGTCCACTATGACGGAAGAGAGTGGAACGAACCATTGAAGTCGTGCAATGGTCAAACTCAGCCGTTCATGGGACAAAGATATCCAGGAGGATTGCCCTTAGGTTGGGTTGTGGTTAACAAAGTGTTGAGCCGAATTAGAAAACCGGTCCATCTTCTCGATCTCACTACTCTCTCGGAGTATCGCAAAGACGCACATCCAAGTCTCTACAATGGTATCTCAAAGGATCTAGACTGTAGCCATTGGTGCCTCCCTGGACTCCCTGATACTTGGAACTTACTTCTCTACTCATCTCTTACTTCCTAG

Protein Analysis

216

Amino Acids

25.08

Weight (kDa)

8.9

Isoelectric Point (pI)

31.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PC-Esterase PF13839 8 - 212 4.1e-66 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 591
AccIII TCCGGA 1 cut(s) 188
AclWI GGATC 1 cut(s) 579
AcsI RAATTY 1 cut(s) 43
AcuI CTGAAG 1 cut(s) 179
AfaI GTAC 2 cut(s) 233, 319
AfiI CCNNNNNNNGG 3 cut(s) 132, 416, 450
AgeI ACCGGT 1 cut(s) 490
AgsI TTSAA 4 cut(s) 80, 160, 335, 385
AjnI CCWGG 3 cut(s) 200, 430, 598
Alw26I GTCTC 1 cut(s) 554
AlwI GGATC 1 cut(s) 579
Aor13HI TCCGGA 1 cut(s) 188
ApoI RAATTY 1 cut(s) 43
ArsI GACNNNNNNTTYG 2 cut(s) 28, 60
AsiGI ACCGGT 1 cut(s) 490
AspS9I GGNCC 1 cut(s) 493
AvaII GGWCC 1 cut(s) 493
AxyI CCTNAGG 1 cut(s) 445
BaeI ACNNNNGTAYC 2 cut(s) 544, 577
BanI GGYRCC 1 cut(s) 591
BanII GRGCYC 1 cut(s) 286
BauI CACGAG 1 cut(s) 163
BbsI GAAGAC 1 cut(s) 319
BccI CCATC 2 cut(s) 122, 504
BceAI ACGGC 1 cut(s) 394
BciT130I CCWGG 3 cut(s) 202, 432, 600
BcoDI GTCTC 1 cut(s) 554
BfaI CTAG 3 cut(s) 65, 575, 649
BfmI CTRYAG 1 cut(s) 580
Bme1390I CCNGG 3 cut(s) 202, 432, 600
Bme18I GGWCC 1 cut(s) 493
BmgT120I GGNCC 1 cut(s) 493
BmiI GGNNCC 1 cut(s) 593
BmrFI CCNGG 3 cut(s) 202, 432, 600
BmsI GCATC 1 cut(s) 118
BpiI GAAGAC 1 cut(s) 319
BpuEI CTTGAG 1 cut(s) 306
BsaAI YACGTR 1 cut(s) 317
BsaJI CCNNGG 3 cut(s) 200, 201, 598
BsaWI WCCGGW 2 cut(s) 188, 490
Bsc4I CCNNNNNNNGG 3 cut(s) 132, 416, 450
Bse118I RCCGGY 1 cut(s) 490
Bse1I ACTGG 1 cut(s) 176
Bse21I CCTNAGG 1 cut(s) 445
Bse3DI GCAATG 1 cut(s) 400
BseAI TCCGGA 1 cut(s) 188
BseBI CCWGG 3 cut(s) 202, 432, 600
BseDI CCNNGG 3 cut(s) 200, 201, 598
BseGI GGATG 2 cut(s) 151, 541
BseLI CCNNNNNNNGG 3 cut(s) 132, 416, 450
BseMI GCAATG 1 cut(s) 400
BseMII CTCAG 1 cut(s) 419
BseNI ACTGG 1 cut(s) 176
BshNI GGYRCC 1 cut(s) 591
BshTI ACCGGT 1 cut(s) 490
BsiSI CCGG 2 cut(s) 189, 491
BslFI GGGAC 2 cut(s) 293, 432
BslI CCNNNNNNNGG 3 cut(s) 132, 416, 450
BsmAI GTCTC 1 cut(s) 554
BsmFI GGGAC 2 cut(s) 293, 432
Bsp1286I GDGCHC 1 cut(s) 286
Bsp13I TCCGGA 1 cut(s) 188
Bsp1407I TGTACA 1 cut(s) 231
Bsp143I GATC 2 cut(s) 505, 571
BspCNI CTCAG 1 cut(s) 418
BspEI TCCGGA 1 cut(s) 188
BspLI GGNNCC 1 cut(s) 593
BspPI GGATC 1 cut(s) 579
BspT107I GGYRCC 1 cut(s) 591
BsrDI GCAATG 1 cut(s) 400
BsrFI RCCGGY 1 cut(s) 490
BsrGI TGTACA 1 cut(s) 231
BsrI ACTGG 1 cut(s) 176
BssAI RCCGGY 1 cut(s) 490
BssECI CCNNGG 3 cut(s) 200, 201, 598
BssMI GATC 2 cut(s) 505, 571
BssSI CACGAG 1 cut(s) 163
Bst2BI CACGAG 1 cut(s) 163
Bst2UI CCWGG 3 cut(s) 202, 432, 600
Bst4CI ACNGT 3 cut(s) 7, 60, 581
Bst6I CTCTTC 1 cut(s) 358
BstAUI TGTACA 1 cut(s) 231
BstBAI YACGTR 1 cut(s) 317
BstDEI CTNAG 2 cut(s) 405, 445
BstF5I GGATG 2 cut(s) 151, 541
BstKTI GATC 2 cut(s) 508, 574
BstMAI GTCTC 1 cut(s) 554
BstMBI GATC 2 cut(s) 505, 571
BstMWI GCNNNNNNNGC 1 cut(s) 591
BstNI CCWGG 3 cut(s) 202, 432, 600
BstSCI CCNGG 3 cut(s) 200, 430, 598
BstSFI CTRYAG 1 cut(s) 580
BstV2I GAAGAC 1 cut(s) 319
BstX2I RGATCY 1 cut(s) 571
BstYI RGATCY 1 cut(s) 571
Bsu36I CCTNAGG 1 cut(s) 445
BtsCI GGATG 2 cut(s) 151, 541
BtsIMutI CAGTG 1 cut(s) 169
Cfr10I RCCGGY 1 cut(s) 490
Cfr13I GGNCC 1 cut(s) 493
CseI GACGC 1 cut(s) 545
Csp6I GTAC 2 cut(s) 232, 318
CspAI ACCGGT 1 cut(s) 490
CviAII CATG 4 cut(s) 142, 241, 279, 415
CviJI RGCY 9 cut(s) 125, 136, 199, 250, 257, 284, 409, 477, 585
CviKI_1 RGCY 9 cut(s) 125, 136, 199, 250, 257, 284, 409, 477, 585
CviQI GTAC 2 cut(s) 232, 318
DdeI CTNAG 2 cut(s) 405, 445
DpnI GATC 2 cut(s) 507, 573
DpnII GATC 2 cut(s) 505, 571
Eam1104I CTCTTC 1 cut(s) 358
EarI CTCTTC 1 cut(s) 358
Eco24I GRGCYC 1 cut(s) 286
Eco32I GATATC 1 cut(s) 428
Eco47I GGWCC 1 cut(s) 493
Eco57I CTGAAG 1 cut(s) 179
Eco81I CCTNAGG 1 cut(s) 445
EcoRII CCWGG 3 cut(s) 200, 430, 598
EcoRV GATATC 1 cut(s) 428
EcoT38I GRGCYC 1 cut(s) 286
FaeI CATG 4 cut(s) 145, 244, 282, 418
FaiI YATR 9 cut(s) 30, 51, 143, 210, 212, 242, 280, 357, 416
FaqI GGGAC 2 cut(s) 293, 432
FatI CATG 4 cut(s) 141, 240, 278, 414
FokI GGATG 2 cut(s) 158, 528
FriOI GRGCYC 1 cut(s) 286
FspBI CTAG 3 cut(s) 65, 575, 649
HapII CCGG 2 cut(s) 189, 491
HgaI GACGC 1 cut(s) 545
Hin1II CATG 4 cut(s) 145, 244, 282, 418
HincII GTYRAC 1 cut(s) 463
HindII GTYRAC 1 cut(s) 463
HinfI GANTC 3 cut(s) 110, 178, 603
HpaI GTTAAC 1 cut(s) 463
HpaII CCGG 2 cut(s) 189, 491
Hpy166II GTNNAC 3 cut(s) 177, 352, 463
Hpy188I TCNGA 1 cut(s) 523
Hpy188III TCNNGA 4 cut(s) 80, 189, 503, 575
Hpy8I GTNNAC 3 cut(s) 177, 352, 463
HpyAV CCTTC 2 cut(s) 212, 321
HpyCH4III ACNGT 3 cut(s) 7, 60, 581
HpyCH4IV ACGT 1 cut(s) 316
HpyCH4V TGCA 1 cut(s) 393
HpyF10VI GCNNNNNNNGC 1 cut(s) 591
HpyF3I CTNAG 2 cut(s) 405, 445
HpySE526I ACGT 1 cut(s) 316
Hsp92II CATG 4 cut(s) 145, 244, 282, 418
Kpn2I TCCGGA 1 cut(s) 188
KspAI GTTAAC 1 cut(s) 463
Kzo9I GATC 2 cut(s) 505, 571
LweI GCATC 1 cut(s) 118
MaeI CTAG 3 cut(s) 65, 575, 649
MaeII ACGT 1 cut(s) 316
MaeIII GTNAC 1 cut(s) 34
MalI GATC 2 cut(s) 507, 573
MboI GATC 2 cut(s) 505, 571
MboII GAAGA 4 cut(s) 148, 319, 375, 491
MfeI CAATTG 1 cut(s) 227
MflI RGATCY 1 cut(s) 571
MhlI GDGCHC 1 cut(s) 286
MluCI AATT 3 cut(s) 43, 227, 480
MlyI GAGTC 3 cut(s) 104, 172, 597
MmeI TCCRAC 1 cut(s) 269
MnlI CCTC 4 cut(s) 196, 207, 428, 605
MroI TCCGGA 1 cut(s) 188
MseI TTAA 3 cut(s) 273, 294, 462
MspI CCGG 2 cut(s) 189, 491
MspR9I CCNGG 3 cut(s) 202, 432, 600
MunI CAATTG 1 cut(s) 227
MvaI CCWGG 3 cut(s) 202, 432, 600
MwoI GCNNNNNNNGC 1 cut(s) 591
NdeII GATC 2 cut(s) 505, 571
NlaIII CATG 4 cut(s) 145, 244, 282, 418
NlaIV GGNNCC 1 cut(s) 593
NmuCI GTSAC 1 cut(s) 34
PasI CCCWGGG 1 cut(s) 201
PfoI TCCNGGA 1 cut(s) 430
PinAI ACCGGT 1 cut(s) 490
PleI GAGTC 3 cut(s) 104, 172, 597
PpsI GAGTC 3 cut(s) 104, 172, 597
Ppu21I YACGTR 1 cut(s) 317
Psp6I CCWGG 3 cut(s) 200, 430, 598
PspGI CCWGG 3 cut(s) 200, 430, 598
PspN4I GGNNCC 1 cut(s) 593
PspPI GGNCC 1 cut(s) 493
PsrI GAACNNNNNNTAC 2 cut(s) 60, 92
PsuI RGATCY 1 cut(s) 571
RsaI GTAC 2 cut(s) 233, 319
RsaNI GTAC 2 cut(s) 232, 318
SaqAI TTAA 3 cut(s) 273, 294, 462
Sau3AI GATC 2 cut(s) 505, 571
Sau96I GGNCC 1 cut(s) 493
SchI GAGTC 3 cut(s) 104, 172, 597
ScrFI CCNGG 3 cut(s) 202, 432, 600
SduI GDGCHC 1 cut(s) 286
SetI ASST 4 cut(s) 188, 319, 340, 451
SfaNI GCATC 1 cut(s) 118
SfcI CTRYAG 1 cut(s) 580
SinI GGWCC 1 cut(s) 493
SmlI CTYRAG 1 cut(s) 321
SmoI CTYRAG 1 cut(s) 321
Sse9I AATT 3 cut(s) 43, 227, 480
SspMI CTAG 3 cut(s) 65, 575, 649
StyD4I CCNGG 3 cut(s) 200, 430, 598
TaaI ACNGT 3 cut(s) 7, 60, 581
TaiI ACGT 1 cut(s) 319
TaqI TCGA 1 cut(s) 504
TasI AATT 3 cut(s) 43, 227, 480
TatI WGTACW 1 cut(s) 231
Tru1I TTAA 3 cut(s) 273, 294, 462
Tru9I TTAA 3 cut(s) 273, 294, 462
TscAI CASTG 1 cut(s) 176
TseFI GTSAC 1 cut(s) 34
Tsp45I GTSAC 1 cut(s) 34
TspDTI ATGAA 2 cut(s) 257, 403
TspGWI ACGGA 1 cut(s) 375
TspRI CASTG 1 cut(s) 176
VpaK11BI GGWCC 1 cut(s) 493
XapI RAATTY 1 cut(s) 43
XbaI TCTAGA 1 cut(s) 574
XspI CTAG 3 cut(s) 65, 575, 649
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.