Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0007940

Physical Location & Seq

Genomic Coordinates Forward (+)

28120210 .. 28123846

Gene Structure 3637 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1232900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1131 bp

ATGGGTTTCCTATTCAGAGCTCTGCTTTTAACTCTGTTTTTAGTTTCCCACCAAACACAAGCACAGGAATTCAATTCCTCTTTCTCCTCCAATAATGCTTCTTCTGCTTCTGCTACCAGTACTACTAGTACTACCAGCTCAGTCGCCAGAAGGCTCGCAGGAAAATGCAACTGGTTCCGCGGTACATGGGTCTACGATGCTTCTGCTAAACCATCCTACTATTCCTCCACCTGTCCCTTCTTAGATCCACAGTTCGACTGCCTAAAGTACGGCCGCCGCGACACCGCTTTCCTCAAATACAGATGGCAACCCTTCTCCTGCGCCCTTCCCAGGTTCAATGGGTTGTATTTCTTGGAGAAATGGAGGGGGAAGAAGATCATGTTTGTGGGTGACTCAATGAGTTTCAATCATTGGGTGTCTTTAACATGTATGCTTCATGCCTGGGTGCCAAATTCCAGGACTTCATATGTTAAGACAAATAGTTTATCTTCAGTCACATTCCAGGACTATGGAGTACAAATACTGCTGTATCGCACACCATACCTGGTAGATCTTGTCAACGAGAACGTGGGACGAGTGTTGAAGCTTGACTCAATTACGAATGGCAAAGCGTGGAGAGGCATGGACATGCTGATCTTCGACACGTGGCACTGGTGGACTCACACCGGAAGAACACAGCCATGGGACTACCTGGAAGAAAGGGGTAAATTGTACAAAGATATGAACCGTCTGGTCGCATTTTACAAGGGAATGACAACTTGGGGCAGATGGGTCGACCGATATGTCGATCCTTCCAAAACCAAGGTCTTCTTCCAAGGCATTTCTCCTACCCATTACGAGGGCAAGGAGTGGAATCAGCCAACAAGATCCTGCGCAAGTGAAACGCAACCATTCTTTGAGAAAAAGTATCCAGCAGGAGTACCTTTGTCTTGGGTTGTAGTCAACAAAGTGCTGGCTAGGATTAAGAAGCCAGTCTATCTTCTCGACATCACGCTTCTTTCACAAATCCGCAAAGATGCACACCCATCCAAGTACAGCGGCCACCACGGCGGCACGGACTGCAGCCACTGGTGCCTTCCCGGTCTGCCGGATACTTGGAATCAGCTCCTCTATGCTGCTCTATTTGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005794 GO:0005911 GO:0008150 GO:0008374 GO:0009506 GO:0009987 GO:0012505 GO:0016407 GO:0016413 GO:0016740 GO:0016746 GO:0016747 GO:0030054 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0055044 GO:0071554

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PC-Esterase PMR5N

Protein Analysis

377

Amino Acids

43.45

Weight (kDa)

9.49

Isoelectric Point (pI)

28.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMR5N	PF14416	55 - 107	2e-20	PMR5 N terminal Domain
PC-Esterase	PF13839	109 - 372	9.7e-95	GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007940)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G31110 AT2G42570
fragaria_vesca	FvH4_6g27120 FvH4_6g27120 FvH4_6g27120
malus_domestica	MD09G1240100.v1.1 MD15G1118000.v1.1 MD16G1270900.v1.1 MD17G1232900.v1.1
prunus_persica	Prupe.3G123200_v2.0.a1 Prupe.3G123200_v2.0.a1
pyrus_communis	pycom09g15850 pycom17g23570 pycom17g23580
rosa_chinensis	RchiOBHm_Chr2g0129071
rosa_laevigata	RLG00000019051
rosa_multiflora	Rmu_sc0004255.1_g000011 Rmu_ssc0000449.1_g000014
rosa_roxburghii	Rroxscaffold_2G00114910
rosa_rugosa	Rorug02G0284000
rosa_samantha	Rh2AG336500 Rh2BG344600 Rh2DG361400
rosa_wichuraiana	Rw2G027290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	782
Acc16I	TGCGCA	1 cut(s)	874
AccB1I	GGYRCC	2 cut(s)	445, 1071
AccI	GTMKAC	2 cut(s)	192, 774
AccII	CGCG	2 cut(s)	180, 279
AciI	CCGC	8 cut(s)	178, 180, 274, 277, 285, 1009, 1038, 1050
AclWI	GGATC	3 cut(s)	239, 782, 861
AcoI	YGGCCR	2 cut(s)	271, 1039
AcsI	RAATTY	2 cut(s)	68, 451
AcuI	CTGAAG	1 cut(s)	474
AcvI	CACGTG	1 cut(s)	645
AfaI	GTAC	8 cut(s)	121, 130, 184, 269, 516, 713, 921, 1034
AfiI	CCNNNNNNNGG	2 cut(s)	330, 838
AflIII	ACRYGT	2 cut(s)	425, 642
AgsI	TTSAA	4 cut(s)	73, 337, 406, 583
AhlI	ACTAGT	1 cut(s)	125
AjnI	CCWGG	6 cut(s)	329, 440, 455, 501, 543, 690
AjuI	GAANNNNNNNTTGG	4 cut(s)	742, 774, 794, 826
AluBI	AGCT	4 cut(s)	20, 138, 586, 1105
AluI	AGCT	4 cut(s)	20, 138, 586, 1105
Alw21I	GWGCWC	1 cut(s)	22
AlwI	GGATC	3 cut(s)	239, 782, 861
AlwNI	CAGNNNCTG	1 cut(s)	1068
AoxI	GGCC	2 cut(s)	271, 1039
ApeKI	GCWGC	2 cut(s)	1062, 1115
ApoI	RAATTY	2 cut(s)	68, 451
ArsI	GACNNNNNNTTYG	2 cut(s)	789, 821
AspLEI	GCGC	2 cut(s)	323, 875
AsuC2I	CCSGG	1 cut(s)	1080
AsuHPI	GGTGA	1 cut(s)	401
BaeI	ACNNNNGTAYC	4 cut(s)	174, 207, 512, 545
BanI	GGYRCC	2 cut(s)	445, 1071
BanII	GRGCYC	1 cut(s)	22
BbrPI	CACGTG	1 cut(s)	645
BbsI	GAAGAC	1 cut(s)	799
Bbv12I	GWGCWC	1 cut(s)	22
BbvI	GCAGC	2 cut(s)	1074, 1102
BccI	CCATC	4 cut(s)	220, 297, 762, 1033
BceAI	ACGGC	2 cut(s)	286, 1063
BcgI	CGANNNNNNTGC	4 cut(s)	185, 219, 754, 788
BciT130I	CCWGG	6 cut(s)	331, 442, 457, 503, 545, 692
BciVI	GTATCC	2 cut(s)	918, 1084
BcnI	CCSGG	1 cut(s)	1080
BcuI	ACTAGT	1 cut(s)	125
BfaI	CTAG	2 cut(s)	126, 957
BfmI	CTRYAG	1 cut(s)	1060
BfuI	GTATCC	2 cut(s)	918, 1084
BglI	GCCNNNNNGGC	1 cut(s)	1047
BglII	AGATCT	1 cut(s)	550
BisI	GCNGC	6 cut(s)	274, 277, 1039, 1051, 1063, 1116
BlsI	GCNGC	6 cut(s)	275, 278, 1040, 1052, 1064, 1117
BmcAI	AGTACT	2 cut(s)	121, 130
Bme1390I	CCNGG	7 cut(s)	331, 442, 457, 503, 545, 692, 1080
BmiI	GGNNCC	3 cut(s)	176, 447, 1073
BmrFI	CCNGG	7 cut(s)	331, 442, 457, 503, 545, 692, 1080
BmsI	GCATC	2 cut(s)	187, 1006
BpiI	GAAGAC	1 cut(s)	799
BpuMI	CCSGG	1 cut(s)	1080
BsaAI	YACGTR	1 cut(s)	645
BsaJI	CCNNGG	7 cut(s)	178, 329, 441, 680, 801, 814, 1045
BsaWI	WCCGGW	1 cut(s)	665
BsaXI	ACNNNNNCTCC	2 cut(s)	209, 239
Bsc4I	CCNNNNNNNGG	2 cut(s)	330, 838
Bse1I	ACTGG	5 cut(s)	117, 176, 656, 971, 1073
BseBI	CCWGG	6 cut(s)	331, 442, 457, 503, 545, 692
BseDI	CCNNGG	7 cut(s)	178, 329, 441, 680, 801, 814, 1045
BseGI	GGATG	2 cut(s)	212, 1025
BseLI	CCNNNNNNNGG	2 cut(s)	330, 838
BseMII	CTCAG	1 cut(s)	153
BseNI	ACTGG	5 cut(s)	117, 176, 656, 971, 1073
BseRI	GAGGAG	2 cut(s)	76, 1097
BseX3I	CGGCCG	1 cut(s)	271
BseXI	GCAGC	2 cut(s)	1074, 1102
Bsh1236I	CGCG	2 cut(s)	180, 279
Bsh1285I	CGRYCG	2 cut(s)	274, 778
BshFI	GGCC	2 cut(s)	273, 1041
BshNI	GGYRCC	2 cut(s)	445, 1071
BsiEI	CGRYCG	2 cut(s)	274, 778
BsiHKAI	GWGCWC	1 cut(s)	22
BsiSI	CCGG	3 cut(s)	666, 1080, 1088
BslFI	GGGAC	3 cut(s)	219, 585, 698
BslI	CCNNNNNNNGG	2 cut(s)	330, 838
BsmFI	GGGAC	3 cut(s)	219, 585, 698
BsnI	GGCC	2 cut(s)	273, 1041
Bsp1286I	GDGCHC	1 cut(s)	22
Bsp1407I	TGTACA	1 cut(s)	711
Bsp143I	GATC	6 cut(s)	244, 375, 550, 633, 787, 866
Bsp19I	CCATGG	1 cut(s)	680
BspACI	CCGC	8 cut(s)	178, 180, 274, 277, 285, 1009, 1038, 1050
BspANI	GGCC	2 cut(s)	273, 1041
BspCNI	CTCAG	1 cut(s)	152
BspFNI	CGCG	2 cut(s)	180, 279
BspLI	GGNNCC	3 cut(s)	176, 447, 1073
BspMAI	CTGCAG	1 cut(s)	1064
BspPI	GGATC	3 cut(s)	239, 782, 861
BspT107I	GGYRCC	2 cut(s)	445, 1071
BsrGI	TGTACA	1 cut(s)	711
BsrI	ACTGG	5 cut(s)	117, 176, 656, 971, 1073
BssECI	CCNNGG	7 cut(s)	178, 329, 441, 680, 801, 814, 1045
BssMI	GATC	6 cut(s)	244, 375, 550, 633, 787, 866
BssT1I	CCWWGG	3 cut(s)	680, 801, 814
Bst2UI	CCWGG	6 cut(s)	331, 442, 457, 503, 545, 692
Bst4CI	ACNGT	2 cut(s)	252, 728
BstAPI	GCANNNNNTGC	1 cut(s)	1059
BstAUI	TGTACA	1 cut(s)	711
BstBAI	YACGTR	1 cut(s)	645
BstC8I	GCNNGC	2 cut(s)	156, 954
BstDEI	CTNAG	2 cut(s)	139, 241
BstDSI	CCRYGG	3 cut(s)	178, 680, 1045
BstF5I	GGATG	2 cut(s)	212, 1025
BstFNI	CGCG	2 cut(s)	180, 279
BstHHI	GCGC	2 cut(s)	323, 875
BstKTI	GATC	6 cut(s)	247, 378, 553, 636, 790, 869
BstMBI	GATC	6 cut(s)	244, 375, 550, 633, 787, 866
BstMCI	CGRYCG	2 cut(s)	274, 778
BstMWI	GCNNNNNNNGC	5 cut(s)	104, 1047, 1059, 1071, 1124
BstNI	CCWGG	6 cut(s)	331, 442, 457, 503, 545, 692
BstNSI	RCATGY	2 cut(s)	429, 631
BstSCI	CCNGG	7 cut(s)	329, 440, 455, 501, 543, 690, 1078
BstSFI	CTRYAG	1 cut(s)	1060
BstUI	CGCG	2 cut(s)	180, 279
BstV1I	GCAGC	2 cut(s)	1074, 1102
BstV2I	GAAGAC	1 cut(s)	799
BstX2I	RGATCY	3 cut(s)	244, 550, 866
BstXI	CCANNNNNNTGG	1 cut(s)	509
BstYI	RGATCY	3 cut(s)	244, 550, 866
BstZI	CGGCCG	1 cut(s)	271
BsuI	GTATCC	2 cut(s)	918, 1084
BsuRI	GGCC	2 cut(s)	273, 1041
BtgI	CCRYGG	3 cut(s)	178, 680, 1045
BtsCI	GGATG	2 cut(s)	212, 1025
BtsIMutI	CAGTG	2 cut(s)	649, 1066
Cac8I	GCNNGC	2 cut(s)	156, 954
CaiI	CAGNNNCTG	1 cut(s)	1068
CfoI	GCGC	2 cut(s)	323, 875
Cfr42I	CCGCGG	1 cut(s)	181
CsiI	ACCWGGT	1 cut(s)	543
Csp6I	GTAC	8 cut(s)	120, 129, 183, 268, 515, 712, 920, 1033
CviAII	CATG	7 cut(s)	186, 379, 426, 437, 622, 628, 681
CviQI	GTAC	8 cut(s)	120, 129, 183, 268, 515, 712, 920, 1033
DdeI	CTNAG	2 cut(s)	139, 241
DpnI	GATC	6 cut(s)	246, 377, 552, 635, 789, 868
DpnII	GATC	6 cut(s)	244, 375, 550, 633, 787, 866
DrdI	GACNNNNNNGTC	1 cut(s)	782
DseDI	GACNNNNNNGTC	1 cut(s)	782
EaeI	YGGCCR	2 cut(s)	271, 1039
EagI	CGGCCG	1 cut(s)	271
Ecl136II	GAGCTC	1 cut(s)	20
EclXI	CGGCCG	1 cut(s)	271
Eco130I	CCWWGG	3 cut(s)	680, 801, 814
Eco24I	GRGCYC	1 cut(s)	22
Eco52I	CGGCCG	1 cut(s)	271
Eco53kI	GAGCTC	1 cut(s)	20
Eco57I	CTGAAG	1 cut(s)	474
Eco72I	CACGTG	1 cut(s)	645
EcoICRI	GAGCTC	1 cut(s)	20
EcoRI	GAATTC	1 cut(s)	68
EcoRII	CCWGG	6 cut(s)	329, 440, 455, 501, 543, 690
EcoT14I	CCWWGG	3 cut(s)	680, 801, 814
EcoT38I	GRGCYC	1 cut(s)	22
ErhI	CCWWGG	3 cut(s)	680, 801, 814
FaeI	CATG	7 cut(s)	189, 382, 429, 440, 625, 631, 684
FalI	AAGNNNNNCTT	2 cut(s)	794, 826
FaqI	GGGAC	3 cut(s)	219, 585, 698
FatI	CATG	7 cut(s)	185, 378, 425, 436, 621, 627, 680
FauNDI	CATATG	1 cut(s)	466
FblI	GTMKAC	2 cut(s)	192, 774
Fnu4HI	GCNGC	6 cut(s)	274, 277, 1039, 1051, 1063, 1116
FokI	GGATG	2 cut(s)	199, 1012
FriOI	GRGCYC	1 cut(s)	22
Fsp4HI	GCNGC	6 cut(s)	274, 277, 1039, 1051, 1063, 1116
FspBI	CTAG	2 cut(s)	126, 957
FspI	TGCGCA	1 cut(s)	874
GlaI	GCGC	2 cut(s)	322, 874
GluI	GCNGC	6 cut(s)	274, 277, 1039, 1051, 1063, 1116
HaeIII	GGCC	2 cut(s)	273, 1041
HapII	CCGG	3 cut(s)	666, 1080, 1088
HhaI	GCGC	2 cut(s)	323, 875
Hin1II	CATG	7 cut(s)	189, 382, 429, 440, 625, 631, 684
Hin6I	GCGC	2 cut(s)	321, 873
HinP1I	GCGC	2 cut(s)	321, 873
HincII	GTYRAC	3 cut(s)	559, 775, 943
HindII	GTYRAC	3 cut(s)	559, 775, 943
HindIII	AAGCTT	1 cut(s)	584
HinfI	GANTC	5 cut(s)	392, 590, 658, 853, 1099
HpaII	CCGG	3 cut(s)	666, 1080, 1088
HphI	GGTGA	1 cut(s)	401
Hpy166II	GTNNAC	5 cut(s)	193, 559, 657, 775, 943
Hpy188I	TCNGA	1 cut(s)	17
Hpy188III	TCNNGA	1 cut(s)	983
Hpy8I	GTNNAC	5 cut(s)	193, 559, 657, 775, 943
HpyAV	CCTTC	6 cut(s)	144, 247, 322, 335, 801, 1085
HpyCH4III	ACNGT	2 cut(s)	252, 728
HpyCH4IV	ACGT	2 cut(s)	567, 644
HpyCH4V	TGCA	3 cut(s)	168, 1019, 1062
HpyF10VI	GCNNNNNNNGC	5 cut(s)	104, 1047, 1059, 1071, 1124
HpyF3I	CTNAG	2 cut(s)	139, 241
HpySE526I	ACGT	2 cut(s)	567, 644
Hsp92II	CATG	7 cut(s)	189, 382, 429, 440, 625, 631, 684
HspAI	GCGC	2 cut(s)	321, 873
KspI	CCGCGG	1 cut(s)	181
Kzo9I	GATC	6 cut(s)	244, 375, 550, 633, 787, 866
LmnI	GCTCC	1 cut(s)	1110
Lsp1109I	GCAGC	2 cut(s)	1074, 1102
LweI	GCATC	2 cut(s)	187, 1006
MabI	ACCWGGT	1 cut(s)	543
MaeI	CTAG	2 cut(s)	126, 957
MaeII	ACGT	2 cut(s)	567, 644
MaeIII	GTNAC	2 cut(s)	389, 493
MalI	GATC	6 cut(s)	246, 377, 552, 635, 789, 868
MboI	GATC	6 cut(s)	244, 375, 550, 633, 787, 866
MflI	RGATCY	3 cut(s)	244, 550, 866
MhlI	GDGCHC	1 cut(s)	22
MluCI	AATT	5 cut(s)	68, 73, 451, 594, 707
MlyI	GAGTC	3 cut(s)	386, 584, 652
MnlI	CCTC	8 cut(s)	88, 97, 235, 302, 357, 611, 832, 1118
MseI	TTAA	4 cut(s)	29, 422, 471, 963
MslI	CAYNNNNRTG	3 cut(s)	383, 626, 679
MspA1I	CMGCKG	2 cut(s)	180, 1038
MspI	CCGG	3 cut(s)	666, 1080, 1088
MspR9I	CCNGG	7 cut(s)	331, 442, 457, 503, 545, 692, 1080
MvaI	CCWGG	6 cut(s)	331, 442, 457, 503, 545, 692
MvnI	CGCG	2 cut(s)	180, 279
MwoI	GCNNNNNNNGC	5 cut(s)	104, 1047, 1059, 1071, 1124
NciI	CCSGG	1 cut(s)	1080
NcoI	CCATGG	1 cut(s)	680
NdeI	CATATG	1 cut(s)	466
NdeII	GATC	6 cut(s)	244, 375, 550, 633, 787, 866
NlaIII	CATG	7 cut(s)	189, 382, 429, 440, 625, 631, 684
NlaIV	GGNNCC	3 cut(s)	176, 447, 1073
NmuCI	GTSAC	2 cut(s)	389, 493
NsbI	TGCGCA	1 cut(s)	874
NspI	RCATGY	2 cut(s)	429, 631
PciI	ACATGT	1 cut(s)	425
PcsI	WCGNNNNNNNCGW	1 cut(s)	276
PfeI	GAWTC	2 cut(s)	853, 1099
PfoI	TCCNGGA	2 cut(s)	455, 501
PkrI	GCNGC	6 cut(s)	275, 278, 1040, 1052, 1064, 1117
PleI	GAGTC	3 cut(s)	386, 584, 652
PmaCI	CACGTG	1 cut(s)	645
PmlI	CACGTG	1 cut(s)	645
PpsI	GAGTC	3 cut(s)	386, 584, 652
Ppu21I	YACGTR	1 cut(s)	645
PscI	ACATGT	1 cut(s)	425
Psp124BI	GAGCTC	1 cut(s)	22
Psp6I	CCWGG	6 cut(s)	329, 440, 455, 501, 543, 690
PspCI	CACGTG	1 cut(s)	645
PspGI	CCWGG	6 cut(s)	329, 440, 455, 501, 543, 690
PspN4I	GGNNCC	3 cut(s)	176, 447, 1073
PstI	CTGCAG	1 cut(s)	1064
PstNI	CAGNNNCTG	1 cut(s)	1068
PsuI	RGATCY	3 cut(s)	244, 550, 866
RsaI	GTAC	8 cut(s)	121, 130, 184, 269, 516, 713, 921, 1034
RsaNI	GTAC	8 cut(s)	120, 129, 183, 268, 515, 712, 920, 1033
RseI	CAYNNNNRTG	3 cut(s)	383, 626, 679
SacI	GAGCTC	1 cut(s)	22
SacII	CCGCGG	1 cut(s)	181
SalI	GTCGAC	1 cut(s)	773
SaqAI	TTAA	4 cut(s)	29, 422, 471, 963
SatI	GCNGC	6 cut(s)	274, 277, 1039, 1051, 1063, 1116
Sau3AI	GATC	6 cut(s)	244, 375, 550, 633, 787, 866
ScaI	AGTACT	2 cut(s)	121, 130
SchI	GAGTC	3 cut(s)	386, 584, 652
ScrFI	CCNGG	7 cut(s)	331, 442, 457, 503, 545, 692, 1080
SduI	GDGCHC	1 cut(s)	22
SexAI	ACCWGGT	1 cut(s)	543
SfaNI	GCATC	2 cut(s)	187, 1006
SfcI	CTRYAG	1 cut(s)	1060
Sfr303I	CCGCGG	1 cut(s)	181
SgrBI	CCGCGG	1 cut(s)	181
SmiMI	CAYNNNNRTG	3 cut(s)	383, 626, 679
SpeI	ACTAGT	1 cut(s)	125
Sse9I	AATT	5 cut(s)	68, 73, 451, 594, 707
SsiI	CCGC	8 cut(s)	178, 180, 274, 277, 285, 1009, 1038, 1050
SspMI	CTAG	2 cut(s)	126, 957
SstI	GAGCTC	1 cut(s)	22
StyD4I	CCNGG	7 cut(s)	329, 440, 455, 501, 543, 690, 1078
StyI	CCWWGG	3 cut(s)	680, 801, 814
TaaI	ACNGT	2 cut(s)	252, 728
TaiI	ACGT	2 cut(s)	570, 647
TaqI	TCGA	5 cut(s)	255, 639, 774, 786, 984
TaqII	GACCGA	1 cut(s)	792
TasI	AATT	5 cut(s)	68, 73, 451, 594, 707
TatI	WGTACW	5 cut(s)	119, 128, 514, 711, 1032
TauI	GCSGC	4 cut(s)	276, 279, 1041, 1053
TfiI	GAWTC	2 cut(s)	853, 1099
Tru1I	TTAA	4 cut(s)	29, 422, 471, 963
Tru9I	TTAA	4 cut(s)	29, 422, 471, 963
TscAI	CASTG	2 cut(s)	656, 1073
TseFI	GTSAC	2 cut(s)	389, 493
TseI	GCWGC	2 cut(s)	1062, 1115
Tsp45I	GTSAC	2 cut(s)	389, 493
TspDTI	ATGAA	3 cut(s)	425, 453, 737
TspGWI	ACGGA	1 cut(s)	1070
TspRI	CASTG	2 cut(s)	656, 1073
XapI	RAATTY	2 cut(s)	68, 451
XceI	RCATGY	2 cut(s)	429, 631
XmiI	GTMKAC	2 cut(s)	192, 774
XspI	CTAG	2 cut(s)	126, 957
ZrmI	AGTACT	2 cut(s)	121, 130

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.