MD15G1118000.v1.1

Protein trichome birefringence-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0007940
Physical Location & Seq
Forward (+)
8480618 .. 8481154
537 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1118000.v1.1.491

Sequence Viewer

Length: 537 bp
ATGGACGTCCTGATCTTCAACTCTTGGCATTGGTGGACCCACACCGGCAAGTCTCAGCCATTTGATTATTTTCGTGATGGGACAAAACCGTACAAAGACATGGATCGTTTGACAGCATATTACAAGGGGCTTTCCACATGGGCTAGATGGGTTGATTCAAATGTTGATACTTCAAGAACCAAAGTCTTCTTCCAGGGCATTTCTCCAACACATTATCAGGGAAAAGAATGTAATTCGCCAAAGAAGACTTGCTCAGGAGAACTTGGACCGTTGTCAGGATCAACCTACCCAGCAGGGGCACCTCCATCTGCTGTTGTTGTCTACAAGGTGCTAAGCACGATTAAGAACCCGGTTTACTTGCTCGACATTACAACGCTCTCGCAGTTGAGAAATGATGCTCATCCCTCAACTTATAGTGGCGATCATTCGGGCAATAACTGCAGCCACTGGTGCCTGCCCAGGTTGCCTGATACCTGGAACCAACTCCTATATGCAGCTCTCCATTTCTTTCTGCCCAGCGAGGAGACTCCGACCTAA

Protein Analysis

179

Amino Acids

20.17

Weight (kDa)

7.71

Isoelectric Point (pI)

33.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PC-Esterase PF13839 1 - 165 1.5e-53 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 9
AccB1I GGYRCC 2 cut(s) 298, 450
AccI GTMKAC 1 cut(s) 321
AclWI GGATC 2 cut(s) 111, 286
AcyI GRCGYC 1 cut(s) 6
AfaI GTAC 1 cut(s) 92
AfiI CCNNNNNNNGG 2 cut(s) 275, 295
AgsI TTSAA 3 cut(s) 19, 159, 174
AjnI CCWGG 3 cut(s) 192, 458, 473
AjuI GAANNNNNNNTTGG 2 cut(s) 173, 205
AluBI AGCT 1 cut(s) 497
AluI AGCT 1 cut(s) 497
Alw26I GTCTC 2 cut(s) 57, 518
AlwI GGATC 2 cut(s) 111, 286
AlwNI CAGNNNCTG 1 cut(s) 447
ApeKI GCWGC 2 cut(s) 441, 494
AspS9I GGNCC 2 cut(s) 36, 266
AsuC2I CCSGG 1 cut(s) 350
AvaII GGWCC 2 cut(s) 36, 266
BaeGI GKGCMC 1 cut(s) 301
BanI GGYRCC 2 cut(s) 298, 450
BbsI GAAGAC 2 cut(s) 178, 251
BbvI GCAGC 2 cut(s) 453, 506
BccI CCATC 3 cut(s) 71, 141, 313
BciT130I CCWGG 3 cut(s) 194, 460, 475
BcnI CCSGG 1 cut(s) 350
BcoDI GTCTC 2 cut(s) 57, 518
BfaI CTAG 1 cut(s) 144
BfmI CTRYAG 1 cut(s) 439
BisI GCNGC 2 cut(s) 442, 495
BlpI GCTNAGC 1 cut(s) 332
BlsI GCNGC 2 cut(s) 443, 496
Bme1390I CCNGG 4 cut(s) 194, 350, 460, 475
Bme18I GGWCC 2 cut(s) 36, 266
BmgT120I GGNCC 2 cut(s) 36, 266
BmiI GGNNCC 4 cut(s) 38, 300, 452, 479
BmrFI CCNGG 4 cut(s) 194, 350, 460, 475
BmsI GCATC 1 cut(s) 385
BoxI GACNNNNGTC 1 cut(s) 271
BpiI GAAGAC 2 cut(s) 178, 251
Bpu10I CCTNAGC 1 cut(s) 253
Bpu1102I GCTNAGC 1 cut(s) 332
BpuMI CCSGG 1 cut(s) 350
BsaBI GATNNNNATC 1 cut(s) 399
BsaHI GRCGYC 1 cut(s) 6
BsaJI CCNNGG 2 cut(s) 193, 458
Bsc4I CCNNNNNNNGG 2 cut(s) 275, 295
Bse118I RCCGGY 1 cut(s) 44
Bse1I ACTGG 1 cut(s) 452
Bse8I GATNNNNATC 1 cut(s) 399
BseBI CCWGG 3 cut(s) 194, 460, 475
BseDI CCNNGG 2 cut(s) 193, 458
BseGI GGATG 1 cut(s) 400
BseJI GATNNNNATC 1 cut(s) 399
BseLI CCNNNNNNNGG 2 cut(s) 275, 295
BseMII CTCAG 2 cut(s) 68, 267
BseNI ACTGG 1 cut(s) 452
BseRI GAGGAG 1 cut(s) 536
BseSI GKGCMC 1 cut(s) 301
BseXI GCAGC 2 cut(s) 453, 506
BseYI CCCAGC 2 cut(s) 289, 515
BshNI GGYRCC 2 cut(s) 298, 450
BsiSI CCGG 2 cut(s) 45, 350
BslFI GGGAC 1 cut(s) 94
BslI CCNNNNNNNGG 2 cut(s) 275, 295
BsmAI GTCTC 2 cut(s) 57, 518
BsmFI GGGAC 1 cut(s) 94
Bsp1286I GDGCHC 1 cut(s) 301
Bsp143I GATC 4 cut(s) 12, 103, 278, 421
Bsp1720I GCTNAGC 1 cut(s) 332
BspCNI CTCAG 2 cut(s) 67, 266
BspLI GGNNCC 4 cut(s) 38, 300, 452, 479
BspMAI CTGCAG 1 cut(s) 443
BspPI GGATC 2 cut(s) 111, 286
BspT107I GGYRCC 2 cut(s) 298, 450
BsrFI RCCGGY 1 cut(s) 44
BsrI ACTGG 1 cut(s) 452
BssAI RCCGGY 1 cut(s) 44
BssECI CCNNGG 2 cut(s) 193, 458
BssMI GATC 4 cut(s) 12, 103, 278, 421
BssNI GRCGYC 1 cut(s) 6
Bst2UI CCWGG 3 cut(s) 194, 460, 475
Bst4CI ACNGT 2 cut(s) 90, 270
BstACI GRCGYC 1 cut(s) 6
BstAPI GCANNNNNTGC 1 cut(s) 438
BstC8I GCNNGC 1 cut(s) 455
BstDEI CTNAG 3 cut(s) 54, 253, 332
BstF5I GGATG 1 cut(s) 400
BstKTI GATC 4 cut(s) 15, 106, 281, 424
BstMAI GTCTC 2 cut(s) 57, 518
BstMBI GATC 4 cut(s) 12, 103, 278, 421
BstMWI GCNNNNNNNGC 3 cut(s) 438, 450, 463
BstNI CCWGG 3 cut(s) 194, 460, 475
BstPAI GACNNNNGTC 1 cut(s) 271
BstSCI CCNGG 4 cut(s) 192, 348, 458, 473
BstSFI CTRYAG 1 cut(s) 439
BstSLI GKGCMC 1 cut(s) 301
BstV1I GCAGC 2 cut(s) 453, 506
BstV2I GAAGAC 2 cut(s) 178, 251
BtsCI GGATG 1 cut(s) 400
BtsIMutI CAGTG 1 cut(s) 445
Cac8I GCNNGC 1 cut(s) 455
CaiI CAGNNNCTG 1 cut(s) 447
Cfr10I RCCGGY 1 cut(s) 44
Cfr13I GGNCC 2 cut(s) 36, 266
Csp6I GTAC 1 cut(s) 91
CviAII CATG 2 cut(s) 100, 138
CviJI RGCY 5 cut(s) 58, 130, 143, 444, 497
CviKI_1 RGCY 5 cut(s) 58, 130, 143, 444, 497
CviQI GTAC 1 cut(s) 91
DdeI CTNAG 3 cut(s) 54, 253, 332
DpnI GATC 4 cut(s) 14, 105, 280, 423
DpnII GATC 4 cut(s) 12, 103, 278, 421
Eco47I GGWCC 2 cut(s) 36, 266
EcoRII CCWGG 3 cut(s) 192, 458, 473
FaeI CATG 2 cut(s) 103, 141
FaiI YATR 6 cut(s) 101, 118, 139, 414, 490, 492
FaqI GGGAC 1 cut(s) 94
FatI CATG 2 cut(s) 99, 137
FblI GTMKAC 1 cut(s) 321
Fnu4HI GCNGC 2 cut(s) 442, 495
FokI GGATG 1 cut(s) 387
Fsp4HI GCNGC 2 cut(s) 442, 495
FspBI CTAG 1 cut(s) 144
GluI GCNGC 2 cut(s) 442, 495
GsaI CCCAGC 2 cut(s) 293, 519
HapII CCGG 2 cut(s) 45, 350
Hin1I GRCGYC 1 cut(s) 6
Hin1II CATG 2 cut(s) 103, 141
HinfI GANTC 2 cut(s) 155, 526
HpaII CCGG 2 cut(s) 45, 350
Hpy166II GTNNAC 3 cut(s) 36, 322, 355
Hpy188I TCNGA 1 cut(s) 531
Hpy188III TCNNGA 5 cut(s) 10, 74, 174, 255, 276
Hpy8I GTNNAC 3 cut(s) 36, 322, 355
HpyCH4III ACNGT 2 cut(s) 90, 270
HpyCH4IV ACGT 1 cut(s) 6
HpyCH4V TGCA 2 cut(s) 441, 494
HpyF10VI GCNNNNNNNGC 3 cut(s) 438, 450, 463
HpyF3I CTNAG 3 cut(s) 54, 253, 332
HpySE526I ACGT 1 cut(s) 6
Hsp92I GRCGYC 1 cut(s) 6
Hsp92II CATG 2 cut(s) 103, 141
Kzo9I GATC 4 cut(s) 12, 103, 278, 421
Lsp1109I GCAGC 2 cut(s) 453, 506
LweI GCATC 1 cut(s) 385
MaeI CTAG 1 cut(s) 144
MaeII ACGT 1 cut(s) 6
MalI GATC 4 cut(s) 14, 105, 280, 423
MboI GATC 4 cut(s) 12, 103, 278, 421
MboII GAAGA 4 cut(s) 7, 178, 181, 256
MhlI GDGCHC 1 cut(s) 301
MluCI AATT 1 cut(s) 232
MlyI GAGTC 1 cut(s) 520
MmeI TCCRAC 1 cut(s) 230
MnlI CCTC 3 cut(s) 312, 415, 514
MseI TTAA 1 cut(s) 342
MspI CCGG 2 cut(s) 45, 350
MspR9I CCNGG 4 cut(s) 194, 350, 460, 475
MvaI CCWGG 3 cut(s) 194, 460, 475
MwoI GCNNNNNNNGC 3 cut(s) 438, 450, 463
NciI CCSGG 1 cut(s) 350
NdeII GATC 4 cut(s) 12, 103, 278, 421
NlaIII CATG 2 cut(s) 103, 141
NlaIV GGNNCC 4 cut(s) 38, 300, 452, 479
PfeI GAWTC 1 cut(s) 155
PkrI GCNGC 2 cut(s) 443, 496
PleI GAGTC 1 cut(s) 520
PpsI GAGTC 1 cut(s) 520
PshAI GACNNNNGTC 1 cut(s) 271
Psp6I CCWGG 3 cut(s) 192, 458, 473
PspFI CCCAGC 2 cut(s) 289, 515
PspGI CCWGG 3 cut(s) 192, 458, 473
PspN4I GGNNCC 4 cut(s) 38, 300, 452, 479
PspPI GGNCC 2 cut(s) 36, 266
PsrI GAACNNNNNNTAC 2 cut(s) 338, 370
PstI CTGCAG 1 cut(s) 443
PstNI CAGNNNCTG 1 cut(s) 447
RsaI GTAC 1 cut(s) 92
RsaNI GTAC 1 cut(s) 91
SaqAI TTAA 1 cut(s) 342
SatI GCNGC 2 cut(s) 442, 495
Sau3AI GATC 4 cut(s) 12, 103, 278, 421
Sau96I GGNCC 2 cut(s) 36, 266
SchI GAGTC 1 cut(s) 520
ScrFI CCNGG 4 cut(s) 194, 350, 460, 475
SduI GDGCHC 1 cut(s) 301
SetI ASST 8 cut(s) 9, 287, 304, 330, 464, 476, 499, 536
SfaNI GCATC 1 cut(s) 385
SfcI CTRYAG 1 cut(s) 439
SinI GGWCC 2 cut(s) 36, 266
Sse9I AATT 1 cut(s) 232
SspMI CTAG 1 cut(s) 144
StyD4I CCNGG 4 cut(s) 192, 348, 458, 473
TaaI ACNGT 2 cut(s) 90, 270
TaiI ACGT 1 cut(s) 9
TaqI TCGA 1 cut(s) 363
TasI AATT 1 cut(s) 232
TfiI GAWTC 1 cut(s) 155
Tru1I TTAA 1 cut(s) 342
Tru9I TTAA 1 cut(s) 342
TscAI CASTG 1 cut(s) 452
TseI GCWGC 2 cut(s) 441, 494
TspRI CASTG 1 cut(s) 452
VpaK11BI GGWCC 2 cut(s) 36, 266
XmiI GTMKAC 1 cut(s) 321
XspI CTAG 1 cut(s) 144
ZraI GACGTC 1 cut(s) 7
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.