AT2G32295

EXS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0003754
Physical Location & Seq
Forward (+)
13718772 .. 13722223
3452 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G32295.1

Sequence Viewer

Length: 1392 bp
ATGTTTGGGGGTGTCTTGTCTATGCCTGTCAATAATCCTCACTTGCGCAAATCTGGAAGTAGACACATCGTCACTAATCTCGGAGATAATGACTTGAAGACTGCAAGTATGCTTCTGAGTACTTATGCGAAATTGCAAACTCCTATATTCCTTCGAAGTCTCAAGGTTGCTCTGTATATTGGTGGTCTTTATGTTTGTGGAAAGGTTGGATTGGAATCTGTAATGAAAATGGGCGTAGAGACAAGAGAACTGTTCTTTTATGAGACATTTCTGTATTATAACCCTCTCCTTCTCATTACGCTGATGGTCTGGCTCTGGGGTGTTAATTTGTGGGTTTTTTCTCGGTCTGGAGTAGATTATGCAGCAATCTTTTATCTAGGATCAGATCATCTTAGTCACAGAGAGATATGGAAGTGTGCCAGGTGGATGACAATAATTATATTGACTAGCATGACTGCCTATCTGTATCTATACTCGCACGGAGATGTAAAGTTGGCTGCATCTCAACCAGTGGTTTTGTATTTCTCAGCTGTGATTATTCTGATAATCCCTTTCAATATCTTCTACATGTCGTCTCGCTACTATTTGCTTTGGACTTTTTGGCGAATACTCTTCCCTGTGCATGCAGTGACTTTTTCAGACTTCTTTCTAGCTGATATCCTGACTTCAATGTCAAAGGTTTTGTCGGATTTAGAACGTTCAGTATGTCGTATGGTCCATCGACAGGTGGCTACTGTTGCATGGTTTGAAGCTGATTCGGTTTGCGGGAGTCATTCTGCTGCAATTCCCTTGGTTCTTGTTCTACCTTATCTTTTCCGGCTGTTCCAATGCATTCGTCAGTACAAAGATAGCAAGGACATTGCAAACATCTACAATGCTGGGAAGTATTTAACAGCGGTGCCTGTCATCTTTCTCTCAGCCCTCAAGTATTATATCGATCCAGATACATGGACTTACTCCATACAGCCGGCATGGATCCTCTCTGGTCTAGCTAACACTTTCTTCTCCTTCTTTTGGGATATATTACGCGATTGGGATCTAAGTGTCTTCACTCGGATTTTCAAATTCACCAGACCAAATCTTTTCTCCCATCTACTATATGGACGCCGTTGGGTATATGTTTGGGTAATCGGAAGCAATCTGGTACTAAGGTGGACATGGACATATAAGTTATCTGCTCATCTCCGTAACAACTACATAACGGTCTTCATCATCACTGCCTTGGAGATTTACAGGCGATTCCAATGGGCGTTTTTCCGTATAGAGAACGTGTGGTATAAAATCAACAATCCCAAGCATACTGCTCATCAATCTAATCCTCTTTCACTCCAACATGATATCGACAGTGAACATGAGAAATTACTCGCGCATAGTCACAGCCCCGGTGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EXS

Protein Analysis

463

Amino Acids

54.05

Weight (kDa)

9.35

Isoelectric Point (pI)

43.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EXS PF03124 90 - 425 6.2e-87 EXS family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 279
AasI GACNNNNNNGTC 1 cut(s) 670
Acc16I TGCGCA 1 cut(s) 47
AccB1I GGYRCC 1 cut(s) 898
AccI GTMKAC 1 cut(s) 61
AccII CGCG 2 cut(s) 1029, 1367
AciI CCGC 2 cut(s) 765, 896
AclI AACGTT 1 cut(s) 697
AclWI GGATC 5 cut(s) 388, 932, 970, 983, 1044
AcsI RAATTY 1 cut(s) 1064
AcyI GRCGYC 1 cut(s) 1105
AfaI GTAC 3 cut(s) 121, 842, 1146
AfiI CCNNNNNNNGG 2 cut(s) 724, 1014
AflIII ACRYGT 2 cut(s) 567, 1269
AgsI TTSAA 5 cut(s) 97, 556, 669, 749, 1063
AhdI GACNNNNNGTC 1 cut(s) 68
AjnI CCWGG 1 cut(s) 419
AluBI AGCT 4 cut(s) 530, 653, 752, 992
AluI AGCT 4 cut(s) 530, 653, 752, 992
Alw26I GTCTC 4 cut(s) 164, 233, 257, 579
AlwI GGATC 5 cut(s) 388, 932, 970, 983, 1044
ApeKI GCWGC 3 cut(s) 362, 497, 779
ApoI RAATTY 1 cut(s) 1064
ArsI GACNNNNNNTTYG 2 cut(s) 668, 700
AspLEI GCGC 2 cut(s) 48, 1369
AspS9I GGNCC 1 cut(s) 715
AsuC2I CCSGG 1 cut(s) 1383
AsuHPI GGTGA 1 cut(s) 1060
AsuII TTCGAA 1 cut(s) 154
AvaII GGWCC 1 cut(s) 715
BaeI ACNNNNGTAYC 2 cut(s) 1136, 1169
BamHI GGATCC 1 cut(s) 975
BanI GGYRCC 1 cut(s) 898
BbsI GAAGAC 3 cut(s) 104, 1039, 1198
BbvI GCAGC 3 cut(s) 374, 484, 766
BccI CCATC 3 cut(s) 298, 726, 1098
BceAI ACGGC 1 cut(s) 1092
BciT130I CCWGG 1 cut(s) 421
BcnI CCSGG 1 cut(s) 1383
BcoDI GTCTC 4 cut(s) 164, 233, 257, 579
BfaI CTAG 4 cut(s) 377, 447, 650, 989
BisI GCNGC 3 cut(s) 363, 498, 780
BlsI GCNGC 3 cut(s) 364, 499, 781
BmcAI AGTACT 1 cut(s) 121
Bme1390I CCNGG 2 cut(s) 421, 1383
Bme18I GGWCC 1 cut(s) 715
BmeRI GACNNNNNGTC 1 cut(s) 68
BmgT120I GGNCC 1 cut(s) 715
BmiI GGNNCC 2 cut(s) 900, 977
BmrFI CCNGG 2 cut(s) 421, 1383
BmsI GCATC 1 cut(s) 509
BpiI GAAGAC 3 cut(s) 104, 1039, 1198
BpmI CTGGAG 1 cut(s) 369
Bpu14I TTCGAA 1 cut(s) 154
BpuEI CTTGAG 2 cut(s) 146, 908
BpuMI CCSGG 1 cut(s) 1383
Bsa29I ATCGAT 1 cut(s) 936
BsaBI GATNNNNATC 2 cut(s) 214, 1035
BsaHI GRCGYC 1 cut(s) 1105
BsaJI CCNNGG 3 cut(s) 789, 1221, 1381
Bsc4I CCNNNNNNNGG 2 cut(s) 724, 1014
Bse118I RCCGGY 1 cut(s) 967
Bse1I ACTGG 1 cut(s) 509
Bse3DI GCAATG 1 cut(s) 858
Bse8I GATNNNNATC 2 cut(s) 214, 1035
BseBI CCWGG 1 cut(s) 421
BseCI ATCGAT 1 cut(s) 936
BseDI CCNNGG 3 cut(s) 789, 1221, 1381
BseGI GGATG 1 cut(s) 432
BseJI GATNNNNATC 2 cut(s) 214, 1035
BseLI CCNNNNNNNGG 2 cut(s) 724, 1014
BseMI GCAATG 1 cut(s) 858
BseMII CTCAG 3 cut(s) 107, 540, 930
BseNI ACTGG 1 cut(s) 509
BseXI GCAGC 3 cut(s) 374, 484, 766
BseYI CCCAGC 1 cut(s) 878
Bsh1236I CGCG 2 cut(s) 1029, 1367
BshNI GGYRCC 1 cut(s) 898
BshVI ATCGAT 1 cut(s) 936
BsiSI CCGG 3 cut(s) 817, 968, 1383
BslI CCNNNNNNNGG 2 cut(s) 724, 1014
BsmAI GTCTC 4 cut(s) 164, 233, 257, 579
BsmBI CGTCTC 1 cut(s) 579
BsmI GAATGC 1 cut(s) 831
Bsp119I TTCGAA 1 cut(s) 154
Bsp143I GATC 5 cut(s) 380, 385, 937, 975, 1036
BspACI CCGC 2 cut(s) 765, 896
BspCNI CTCAG 3 cut(s) 108, 539, 929
BspDI ATCGAT 1 cut(s) 936
BspFNI CGCG 2 cut(s) 1029, 1367
BspLI GGNNCC 2 cut(s) 900, 977
BspPI GGATC 5 cut(s) 388, 932, 970, 983, 1044
BspT104I TTCGAA 1 cut(s) 154
BspT107I GGYRCC 1 cut(s) 898
BsrDI GCAATG 1 cut(s) 858
BsrFI RCCGGY 1 cut(s) 967
BsrI ACTGG 1 cut(s) 509
BssAI RCCGGY 1 cut(s) 967
BssECI CCNNGG 3 cut(s) 789, 1221, 1381
BssMI GATC 5 cut(s) 380, 385, 937, 975, 1036
BssNI GRCGYC 1 cut(s) 1105
BssT1I CCWWGG 2 cut(s) 789, 1221
Bst2UI CCWGG 1 cut(s) 421
Bst4CI ACNGT 4 cut(s) 252, 736, 1204, 1346
Bst6I CTCTTC 1 cut(s) 617
BstACI GRCGYC 1 cut(s) 1105
BstBI TTCGAA 1 cut(s) 154
BstC8I GCNNGC 2 cut(s) 624, 969
BstDEI CTNAG 6 cut(s) 116, 392, 526, 916, 1040, 1148
BstF5I GGATG 1 cut(s) 432
BstFNI CGCG 2 cut(s) 1029, 1367
BstHHI GCGC 2 cut(s) 48, 1369
BstKTI GATC 5 cut(s) 383, 388, 940, 978, 1039
BstMAI GTCTC 4 cut(s) 164, 233, 257, 579
BstMBI GATC 5 cut(s) 380, 385, 937, 975, 1036
BstMWI GCNNNNNNNGC 1 cut(s) 737
BstNI CCWGG 1 cut(s) 421
BstNSI RCATGY 2 cut(s) 571, 626
BstSCI CCNGG 2 cut(s) 419, 1381
BstUI CGCG 2 cut(s) 1029, 1367
BstV1I GCAGC 3 cut(s) 374, 484, 766
BstV2I GAAGAC 3 cut(s) 104, 1039, 1198
BstX2I RGATCY 2 cut(s) 975, 1036
BstXI CCANNNNNNTGG 1 cut(s) 948
BstYI RGATCY 2 cut(s) 975, 1036
Bsu15I ATCGAT 1 cut(s) 936
BsuTUI ATCGAT 1 cut(s) 936
BtsCI GGATG 1 cut(s) 432
BtsI GCAGTG 2 cut(s) 633, 1215
BtsIMutI CAGTG 4 cut(s) 516, 633, 1215, 1351
Cac8I GCNNGC 2 cut(s) 624, 969
CfoI GCGC 2 cut(s) 48, 1369
Cfr10I RCCGGY 1 cut(s) 967
Cfr13I GGNCC 1 cut(s) 715
ClaI ATCGAT 1 cut(s) 936
CseI GACGC 1 cut(s) 1113
Csp6I GTAC 3 cut(s) 120, 841, 1145
CviAII CATG 9 cut(s) 451, 568, 623, 741, 948, 972, 1158, 1334, 1352
CviQI GTAC 3 cut(s) 120, 841, 1145
DdeI CTNAG 6 cut(s) 116, 392, 526, 916, 1040, 1148
DpnI GATC 5 cut(s) 382, 387, 939, 977, 1038
DpnII GATC 5 cut(s) 380, 385, 937, 975, 1036
DrdI GACNNNNNNGTC 1 cut(s) 670
DriI GACNNNNNGTC 1 cut(s) 68
DseDI GACNNNNNNGTC 1 cut(s) 670
Eam1104I CTCTTC 1 cut(s) 617
Eam1105I GACNNNNNGTC 1 cut(s) 68
EarI CTCTTC 1 cut(s) 617
Eco130I CCWWGG 2 cut(s) 789, 1221
Eco32I GATATC 2 cut(s) 658, 1339
Eco47I GGWCC 1 cut(s) 715
EcoRII CCWGG 1 cut(s) 419
EcoRV GATATC 2 cut(s) 658, 1339
EcoT14I CCWWGG 2 cut(s) 789, 1221
EcoT22I ATGCAT 1 cut(s) 833
ErhI CCWWGG 2 cut(s) 789, 1221
Esp3I CGTCTC 1 cut(s) 579
FaeI CATG 9 cut(s) 454, 571, 626, 744, 951, 975, 1161, 1337, 1355
FatI CATG 9 cut(s) 450, 567, 622, 740, 947, 971, 1157, 1333, 1351
FauI CCCGC 1 cut(s) 758
FblI GTMKAC 1 cut(s) 61
Fnu4HI GCNGC 3 cut(s) 363, 498, 780
FokI GGATG 1 cut(s) 439
Fsp4HI GCNGC 3 cut(s) 363, 498, 780
FspBI CTAG 4 cut(s) 377, 447, 650, 989
FspI TGCGCA 1 cut(s) 47
GlaI GCGC 2 cut(s) 47, 1368
GluI GCNGC 3 cut(s) 363, 498, 780
GsaI CCCAGC 1 cut(s) 882
GsuI CTGGAG 1 cut(s) 369
HapII CCGG 3 cut(s) 817, 968, 1383
HgaI GACGC 1 cut(s) 1113
HhaI GCGC 2 cut(s) 48, 1369
Hin1I GRCGYC 1 cut(s) 1105
Hin1II CATG 9 cut(s) 454, 571, 626, 744, 951, 975, 1161, 1337, 1355
Hin6I GCGC 2 cut(s) 46, 1367
HinP1I GCGC 2 cut(s) 46, 1367
HinfI GANTC 4 cut(s) 215, 755, 769, 1239
HpaII CCGG 3 cut(s) 817, 968, 1383
HphI GGTGA 1 cut(s) 1060
Hpy166II GTNNAC 3 cut(s) 62, 1155, 1349
Hpy188I TCNGA 8 cut(s) 83, 117, 385, 543, 640, 688, 1056, 1133
Hpy188III TCNNGA 4 cut(s) 54, 348, 661, 941
Hpy8I GTNNAC 3 cut(s) 62, 1155, 1349
HpyAV CCTTC 3 cut(s) 161, 299, 1018
HpyCH4III ACNGT 4 cut(s) 252, 736, 1204, 1346
HpyCH4IV ACGT 2 cut(s) 697, 1269
HpyF10VI GCNNNNNNNGC 1 cut(s) 737
HpyF3I CTNAG 6 cut(s) 116, 392, 526, 916, 1040, 1148
HpySE526I ACGT 2 cut(s) 697, 1269
Hsp92I GRCGYC 1 cut(s) 1105
Hsp92II CATG 9 cut(s) 454, 571, 626, 744, 951, 975, 1161, 1337, 1355
HspAI GCGC 2 cut(s) 46, 1367
KroI GCCGGC 1 cut(s) 967
KroNI GCCGGC 1 cut(s) 969
Kzo9I GATC 5 cut(s) 380, 385, 937, 975, 1036
Lsp1109I GCAGC 3 cut(s) 374, 484, 766
LweI GCATC 1 cut(s) 509
MaeI CTAG 4 cut(s) 377, 447, 650, 989
MaeII ACGT 2 cut(s) 697, 1269
MaeIII GTNAC 5 cut(s) 70, 395, 628, 1187, 1373
MalI GATC 5 cut(s) 382, 387, 939, 977, 1038
MboI GATC 5 cut(s) 380, 385, 937, 975, 1036
MboII GAAGA 6 cut(s) 109, 553, 604, 994, 1039, 1198
MflI RGATCY 2 cut(s) 975, 1036
MluCI AATT 6 cut(s) 131, 325, 435, 783, 1064, 1358
MlyI GAGTC 1 cut(s) 778
MmeI TCCRAC 3 cut(s) 187, 666, 1354
MnlI CCTC 5 cut(s) 48, 294, 932, 989, 1329
Mph1103I ATGCAT 1 cut(s) 833
MroNI GCCGGC 1 cut(s) 967
MseI TTAA 2 cut(s) 324, 890
MslI CAYNNNNRTG 1 cut(s) 483
MspA1I CMGCKG 2 cut(s) 530, 896
MspI CCGG 3 cut(s) 817, 968, 1383
MspR9I CCNGG 2 cut(s) 421, 1383
Mva1269I GAATGC 1 cut(s) 831
MvaI CCWGG 1 cut(s) 421
MvnI CGCG 2 cut(s) 1029, 1367
MwoI GCNNNNNNNGC 1 cut(s) 737
NaeI GCCGGC 1 cut(s) 969
NciI CCSGG 1 cut(s) 1383
NdeII GATC 5 cut(s) 380, 385, 937, 975, 1036
NgoMIV GCCGGC 1 cut(s) 967
NlaIII CATG 9 cut(s) 454, 571, 626, 744, 951, 975, 1161, 1337, 1355
NlaIV GGNNCC 2 cut(s) 900, 977
NmuCI GTSAC 4 cut(s) 70, 395, 628, 1373
NsbI TGCGCA 1 cut(s) 47
NsiI ATGCAT 1 cut(s) 833
NspI RCATGY 2 cut(s) 571, 626
NspV TTCGAA 1 cut(s) 154
PaeI GCATGC 1 cut(s) 626
PciI ACATGT 1 cut(s) 567
PctI GAATGC 1 cut(s) 831
PdiI GCCGGC 1 cut(s) 969
PfeI GAWTC 3 cut(s) 215, 755, 1239
PkrI GCNGC 3 cut(s) 364, 499, 781
PleI GAGTC 1 cut(s) 777
PpsI GAGTC 1 cut(s) 777
PscI ACATGT 1 cut(s) 567
PsiI TTATAA 1 cut(s) 279
Psp1406I AACGTT 1 cut(s) 697
Psp6I CCWGG 1 cut(s) 419
PspFI CCCAGC 1 cut(s) 878
PspGI CCWGG 1 cut(s) 419
PspN4I GGNNCC 2 cut(s) 900, 977
PspPI GGNCC 1 cut(s) 715
PsuI RGATCY 2 cut(s) 975, 1036
PvuII CAGCTG 1 cut(s) 530
RsaI GTAC 3 cut(s) 121, 842, 1146
RsaNI GTAC 3 cut(s) 120, 841, 1145
RseI CAYNNNNRTG 1 cut(s) 483
SaqAI TTAA 2 cut(s) 324, 890
SatI GCNGC 3 cut(s) 363, 498, 780
Sau3AI GATC 5 cut(s) 380, 385, 937, 975, 1036
Sau96I GGNCC 1 cut(s) 715
ScaI AGTACT 1 cut(s) 121
SchI GAGTC 1 cut(s) 778
ScrFI CCNGG 2 cut(s) 421, 1383
SfaNI GCATC 1 cut(s) 509
SfuI TTCGAA 1 cut(s) 154
SinI GGWCC 1 cut(s) 715
SmiMI CAYNNNNRTG 1 cut(s) 483
SmlI CTYRAG 2 cut(s) 161, 923
SmoI CTYRAG 2 cut(s) 161, 923
SphI GCATGC 1 cut(s) 626
Sse9I AATT 6 cut(s) 131, 325, 435, 783, 1064, 1358
SsiI CCGC 2 cut(s) 765, 896
SspMI CTAG 4 cut(s) 377, 447, 650, 989
StyD4I CCNGG 2 cut(s) 419, 1381
StyI CCWWGG 2 cut(s) 789, 1221
TaaI ACNGT 4 cut(s) 252, 736, 1204, 1346
TaiI ACGT 2 cut(s) 700, 1272
TaqI TCGA 4 cut(s) 154, 721, 936, 1341
TaqII GACCGA 1 cut(s) 333
TasI AATT 6 cut(s) 131, 325, 435, 783, 1064, 1358
TatI WGTACW 2 cut(s) 119, 840
TfiI GAWTC 3 cut(s) 215, 755, 1239
Tru1I TTAA 2 cut(s) 324, 890
Tru9I TTAA 2 cut(s) 324, 890
TscAI CASTG 4 cut(s) 516, 633, 1222, 1351
TseFI GTSAC 4 cut(s) 70, 395, 628, 1373
TseI GCWGC 3 cut(s) 362, 497, 779
Tsp45I GTSAC 4 cut(s) 70, 395, 628, 1373
TspDTI ATGAA 2 cut(s) 239, 1198
TspGWI ACGGA 3 cut(s) 495, 1175, 1247
TspRI CASTG 4 cut(s) 516, 633, 1222, 1351
VpaK11BI GGWCC 1 cut(s) 715
XapI RAATTY 1 cut(s) 1064
XceI RCATGY 2 cut(s) 571, 626
XcmI CCANNNNNNNNNTGG 1 cut(s) 1097
XmiI GTMKAC 1 cut(s) 61
XspI CTAG 4 cut(s) 377, 447, 650, 989
ZrmI AGTACT 1 cut(s) 121
Zsp2I ATGCAT 1 cut(s) 833
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.