Rroxscaffold_2G00090020

SPX and EXS domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0003754
Physical Location & Seq
Forward (+)
11865464 .. 11870771
5308 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00090020.1

Sequence Viewer

Length: 1422 bp
ATGTTTGGAGGTCTTGAGACAGTTCCTTCCAATAGTCCGCACTTAAGAAAGTCGGGTAGCAGACCTGTTGTCTCTGATCTGGGTACAAGTGAAGTTGAGAATGTTGCTGAGGAGGGTTTTGTGCATCTAATAGAGGGAAACGATATGAAGGGTGTAAGCACACCATTGAGTACGGTTGCAATAATGCCGTCTCCGACTTTGCTGTGGAGATTCAAGGTCCTGCTGTTCTTTGCTTGGGGTTTCATTTGTTGCAAGATTGGATGGGATTCTGTCATGAGAATGAGTGTGGACTTGCGAGATTTATTTCTGTACGAGGCATTTTTGTACTACAATCCTCTTCTTCTTGTGACTATGATGGTTTGGTTTTGGGGAATTAATCTATGGGTGTTCGCGCAGTCCCAAGTCAGTTATCCTAAAATTTTTGAGCTTGATCAAAATCACTTGACTCACAGAGAAATATGGAAGTGTGCCACATGGATGACCATTGTTGTGCCAACAAGCATGACGGCGTATCTTTATCTCTACTCACATGGAGAAGTGTCGCTGGCTGCCTCACAACCAGTACTATTATATGCTGCAGTTGCAATGATTTTGATATTCCCTTTTGATATTTTCTATCTGTCAACTCGCTTTTACTTTTTGAGGACTCTTTGGCGTATCGTTCTCCCTCTACAGGCAATATCCTTTTCTGACTTTTTCGTGGCTGATATTCTTACCTCCATGTCGAAGGTGCTTTCAGATTTGGAGCGATCAGTATGTCGAATGCTCCATCGACAGGTTGCCACTATAGCATGGTTTGAAGCTGATTCAGTTTGTGGCAGTCACTCTGTTGCAATCCCTTTGGTTCTTGTGTTGCCGTATCTTTTTCGTTTGTTCCAATGCCTTCGACAGTACAAGGATACTGGAGAGAAAACATCCCTTTTGAATGCTTTAAAGTATTCGACTGCAGTACCTGTGATTTTTCTCTCAGCCCTTAAATATCACGTCTTCCCTGAAAAGTGGACAAACTTCTATCGGCCCCTTTGGCTTCTCTCGGCCATTTTGAACTCTTTGTACTCATTTTACTGGGATGTGACTAGAGATTGGGACTTGAGTGGTTTCACTCGGATTTTCAAGTCCAACAAATCACATATCTTGTCAAACCTCATGCATGGACGGAAATGGGTTTATTTCTGGGTGATAACTAGCAACTTAATACTGCGTTGTACATGGACATACAAGCTGTCTGCCCATCTCCGTCACAATTATCTTACTGTGTTTGCAATTACTGCCTTGGAGATTTTCCGCCGCTTTCAATGGGTTTTCTTCCGTGTGGAAAAGGAGTGGATCAAGATGAGCTCGAAGTCTAACATTCAACTTTCCATGAGTGACATCCCAAATGAGGAAGACAGATTACTTGTTTCTAATGGCCACAGTGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EXS

Protein Analysis

473

Amino Acids

55.1

Weight (kDa)

8.9

Isoelectric Point (pI)

40.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EXS PF03124 107 - 443 1.1e-94 EXS family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 392
AciI CCGC 3 cut(s) 38, 1285, 1288
AclWI GGATC 1 cut(s) 1334
AcoI YGGCCR 2 cut(s) 1035, 1408
AcsI RAATTY 1 cut(s) 417
AfaI GTAC 9 cut(s) 85, 172, 311, 326, 564, 893, 951, 1055, 1207
AfiI CCNNNNNNNGG 3 cut(s) 673, 775, 1381
AflII CTTAAG 1 cut(s) 43
AgsI TTSAA 7 cut(s) 214, 800, 925, 1045, 1114, 1295, 1355
AhdI GACNNNNNGTC 1 cut(s) 68
AjiI CACGTC 1 cut(s) 985
AluBI AGCT 4 cut(s) 427, 803, 1222, 1338
AluI AGCT 4 cut(s) 427, 803, 1222, 1338
Alw21I GWGCWC 1 cut(s) 1340
Alw26I GTCTC 3 cut(s) 11, 76, 195
AlwI GGATC 1 cut(s) 1334
AlwNI CAGNNNCTG 1 cut(s) 953
AoxI GGCC 3 cut(s) 1016, 1035, 1408
ApeKI GCWGC 2 cut(s) 548, 575
ApoI RAATTY 1 cut(s) 417
AseI ATTAAT 1 cut(s) 375
AspLEI GCGC 1 cut(s) 394
AspS9I GGNCC 2 cut(s) 217, 1017
AsuHPI GGTGA 1 cut(s) 1189
AvaII GGWCC 1 cut(s) 217
BaeI ACNNNNGTAYC 2 cut(s) 933, 966
BalI TGGCCA 1 cut(s) 1410
BanII GRGCYC 1 cut(s) 1340
BarI GAAGNNNNNNTAC 2 cut(s) 1377, 1409
BbsI GAAGAC 2 cut(s) 979, 1392
Bbv12I GWGCWC 1 cut(s) 1340
BbvCI CCTCAGC 1 cut(s) 108
BbvI GCAGC 2 cut(s) 535, 562
BccI CCATC 4 cut(s) 255, 349, 777, 1239
BceAI ACGGC 3 cut(s) 172, 522, 841
BciVI GTATCC 1 cut(s) 892
BclI TGATCA 1 cut(s) 430
BcoDI GTCTC 3 cut(s) 11, 76, 195
BfaI CTAG 2 cut(s) 1077, 1185
BfmI CTRYAG 4 cut(s) 576, 671, 786, 945
BfrI CTTAAG 1 cut(s) 43
BfuI GTATCC 1 cut(s) 892
BglI GCCNNNNNGGC 1 cut(s) 1024
BisI GCNGC 3 cut(s) 549, 576, 1288
BlsI GCNGC 3 cut(s) 550, 577, 1289
BmcAI AGTACT 1 cut(s) 564
Bme18I GGWCC 1 cut(s) 217
BmeRI GACNNNNNGTC 1 cut(s) 68
BmgBI CACGTC 1 cut(s) 985
BmgT120I GGNCC 2 cut(s) 217, 1017
BmiI GGNNCC 1 cut(s) 1019
BmrI ACTGGG 1 cut(s) 1075
BmsI GCATC 1 cut(s) 133
BmuI ACTGGG 1 cut(s) 1075
BpiI GAAGAC 2 cut(s) 979, 1392
BpmI CTGGAG 1 cut(s) 924
Bpu10I CCTNAGC 1 cut(s) 108
BpuEI CTTGAG 2 cut(s) 35, 1111
BsaBI GATNNNNATC 1 cut(s) 435
BsaJI CCNNGG 1 cut(s) 1272
Bsc4I CCNNNNNNNGG 3 cut(s) 673, 775, 1381
Bse1I ACTGG 3 cut(s) 560, 907, 1070
Bse3DI GCAATG 1 cut(s) 591
Bse8I GATNNNNATC 1 cut(s) 435
BseDI CCNNGG 1 cut(s) 1272
BseGI GGATG 5 cut(s) 266, 483, 914, 1075, 1371
BseJI GATNNNNATC 1 cut(s) 435
BseLI CCNNNNNNNGG 3 cut(s) 673, 775, 1381
BseMI GCAATG 1 cut(s) 591
BseMII CTCAG 2 cut(s) 99, 981
BseNI ACTGG 3 cut(s) 560, 907, 1070
BseRI GAGGAG 1 cut(s) 125
BseXI GCAGC 2 cut(s) 535, 562
Bsh1236I CGCG 1 cut(s) 392
BshFI GGCC 3 cut(s) 1018, 1037, 1410
BsiHKAI GWGCWC 1 cut(s) 1340
BslFI GGGAC 2 cut(s) 382, 1100
BslI CCNNNNNNNGG 3 cut(s) 673, 775, 1381
BsmAI GTCTC 3 cut(s) 11, 76, 195
BsmBI CGTCTC 1 cut(s) 195
BsmFI GGGAC 2 cut(s) 382, 1100
BsmI GAATGC 2 cut(s) 768, 931
BsnI GGCC 3 cut(s) 1018, 1037, 1410
Bsp1286I GDGCHC 1 cut(s) 1340
Bsp1407I TGTACA 1 cut(s) 1205
Bsp143I GATC 4 cut(s) 76, 430, 749, 1326
BspACI CCGC 3 cut(s) 38, 1285, 1288
BspANI GGCC 3 cut(s) 1018, 1037, 1410
BspCNI CTCAG 2 cut(s) 100, 980
BspFNI CGCG 1 cut(s) 392
BspHI TCATGA 1 cut(s) 273
BspLI GGNNCC 1 cut(s) 1019
BspMAI CTGCAG 2 cut(s) 580, 949
BspPI GGATC 1 cut(s) 1334
BspTI CTTAAG 1 cut(s) 43
BsrDI GCAATG 1 cut(s) 591
BsrGI TGTACA 1 cut(s) 1205
BsrI ACTGG 3 cut(s) 560, 907, 1070
BssECI CCNNGG 1 cut(s) 1272
BssMI GATC 4 cut(s) 76, 430, 749, 1326
BssT1I CCWWGG 1 cut(s) 1272
Bst4CI ACNGT 5 cut(s) 22, 175, 891, 1255, 1415
Bst6I CTCTTC 1 cut(s) 342
BstAFI CTTAAG 1 cut(s) 43
BstAPI GCANNNNNTGC 1 cut(s) 1268
BstAUI TGTACA 1 cut(s) 1205
BstC8I GCNNGC 1 cut(s) 546
BstDEI CTNAG 2 cut(s) 108, 967
BstF5I GGATG 5 cut(s) 266, 483, 914, 1075, 1371
BstFNI CGCG 1 cut(s) 392
BstHHI GCGC 1 cut(s) 394
BstKTI GATC 4 cut(s) 79, 433, 752, 1329
BstMAI GTCTC 3 cut(s) 11, 76, 195
BstMBI GATC 4 cut(s) 76, 430, 749, 1326
BstMWI GCNNNNNNNGC 4 cut(s) 581, 788, 1024, 1268
BstSFI CTRYAG 4 cut(s) 576, 671, 786, 945
BstUI CGCG 1 cut(s) 392
BstV1I GCAGC 2 cut(s) 535, 562
BstV2I GAAGAC 2 cut(s) 979, 1392
BsuI GTATCC 1 cut(s) 892
BsuRI GGCC 3 cut(s) 1018, 1037, 1410
BtrI CACGTC 1 cut(s) 985
BtsCI GGATG 5 cut(s) 266, 483, 914, 1075, 1371
BtsIMutI CAGTG 1 cut(s) 1420
Cac8I GCNNGC 1 cut(s) 546
CaiI CAGNNNCTG 1 cut(s) 953
CciI TCATGA 1 cut(s) 273
CfoI GCGC 1 cut(s) 394
Cfr13I GGNCC 2 cut(s) 217, 1017
Csp6I GTAC 9 cut(s) 84, 171, 310, 325, 563, 892, 950, 1054, 1206
CviQI GTAC 9 cut(s) 84, 171, 310, 325, 563, 892, 950, 1054, 1206
DdeI CTNAG 2 cut(s) 108, 967
DpnI GATC 4 cut(s) 78, 432, 751, 1328
DpnII GATC 4 cut(s) 76, 430, 749, 1326
DraI TTTAAA 1 cut(s) 933
DriI GACNNNNNGTC 1 cut(s) 68
EaeI YGGCCR 2 cut(s) 1035, 1408
Eam1104I CTCTTC 1 cut(s) 342
Eam1105I GACNNNNNGTC 1 cut(s) 68
EarI CTCTTC 1 cut(s) 342
EciI GGCGGA 1 cut(s) 1274
Ecl136II GAGCTC 1 cut(s) 1338
Eco130I CCWWGG 1 cut(s) 1272
Eco24I GRGCYC 1 cut(s) 1340
Eco47I GGWCC 1 cut(s) 217
Eco53kI GAGCTC 1 cut(s) 1338
EcoICRI GAGCTC 1 cut(s) 1338
EcoO109I RGGNCCY 1 cut(s) 217
EcoT14I CCWWGG 1 cut(s) 1272
EcoT22I ATGCAT 1 cut(s) 1152
EcoT38I GRGCYC 1 cut(s) 1340
ErhI CCWWGG 1 cut(s) 1272
Esp3I CGTCTC 1 cut(s) 195
FaqI GGGAC 2 cut(s) 382, 1100
FbaI TGATCA 1 cut(s) 430
Fnu4HI GCNGC 3 cut(s) 549, 576, 1288
FokI GGATG 5 cut(s) 273, 490, 901, 1082, 1358
FriOI GRGCYC 1 cut(s) 1340
Fsp4HI GCNGC 3 cut(s) 549, 576, 1288
FspBI CTAG 2 cut(s) 1077, 1185
GlaI GCGC 1 cut(s) 393
GluI GCNGC 3 cut(s) 549, 576, 1288
GsuI CTGGAG 1 cut(s) 924
HaeIII GGCC 3 cut(s) 1018, 1037, 1410
HhaI GCGC 1 cut(s) 394
Hin6I GCGC 1 cut(s) 392
HinP1I GCGC 1 cut(s) 392
HincII GTYRAC 1 cut(s) 624
HindII GTYRAC 1 cut(s) 624
HinfI GANTC 5 cut(s) 210, 266, 445, 646, 806
HphI GGTGA 1 cut(s) 1189
Hpy166II GTNNAC 3 cut(s) 289, 624, 1002
Hpy188I TCNGA 5 cut(s) 76, 195, 691, 739, 1107
Hpy188III TCNNGA 3 cut(s) 14, 274, 1330
Hpy8I GTNNAC 3 cut(s) 289, 624, 1002
HpyAV CCTTC 4 cut(s) 36, 142, 721, 893
HpyCH4III ACNGT 5 cut(s) 22, 175, 891, 1255, 1415
HpyCH4IV ACGT 1 cut(s) 984
HpyCH4V TGCA 9 cut(s) 124, 179, 252, 578, 584, 833, 947, 1150, 1262
HpyF10VI GCNNNNNNNGC 4 cut(s) 581, 788, 1024, 1268
HpyF3I CTNAG 2 cut(s) 108, 967
HpySE526I ACGT 1 cut(s) 984
HspAI GCGC 1 cut(s) 392
Ksp22I TGATCA 1 cut(s) 430
Kzo9I GATC 4 cut(s) 76, 430, 749, 1326
LmnI GCTCC 2 cut(s) 745, 771
Lsp1109I GCAGC 2 cut(s) 535, 562
LweI GCATC 1 cut(s) 133
MaeI CTAG 2 cut(s) 1077, 1185
MaeII ACGT 1 cut(s) 984
MaeIII GTNAC 5 cut(s) 346, 821, 1072, 1238, 1367
MalI GATC 4 cut(s) 78, 432, 751, 1328
MboI GATC 4 cut(s) 76, 430, 749, 1326
MboII GAAGA 5 cut(s) 329, 332, 979, 1297, 1397
MhlI GDGCHC 1 cut(s) 1340
MlsI TGGCCA 1 cut(s) 1410
MluCI AATT 4 cut(s) 372, 417, 1243, 1263
MluNI TGGCCA 1 cut(s) 1410
MlyI GAGTC 2 cut(s) 439, 640
MmeI TCCRAC 2 cut(s) 218, 1143
Mox20I TGGCCA 1 cut(s) 1410
Mph1103I ATGCAT 1 cut(s) 1152
MscI TGGCCA 1 cut(s) 1410
MseI TTAA 5 cut(s) 44, 375, 932, 975, 1193
MslI CAYNNNNRTG 3 cut(s) 278, 476, 488
Msp20I TGGCCA 1 cut(s) 1410
MspCI CTTAAG 1 cut(s) 43
Mva1269I GAATGC 2 cut(s) 768, 931
MvnI CGCG 1 cut(s) 392
MwoI GCNNNNNNNGC 4 cut(s) 581, 788, 1024, 1268
NdeII GATC 4 cut(s) 76, 430, 749, 1326
NlaIV GGNNCC 1 cut(s) 1019
NmeAIII GCCGAG 1 cut(s) 1013
NmuCI GTSAC 5 cut(s) 346, 821, 1072, 1238, 1367
NsiI ATGCAT 1 cut(s) 1152
PagI TCATGA 1 cut(s) 273
PctI GAATGC 2 cut(s) 768, 931
PfeI GAWTC 3 cut(s) 210, 266, 806
PkrI GCNGC 3 cut(s) 550, 577, 1289
PleI GAGTC 2 cut(s) 439, 640
PpsI GAGTC 2 cut(s) 439, 640
PpuMI RGGWCCY 1 cut(s) 217
PshBI ATTAAT 1 cut(s) 375
Psp124BI GAGCTC 1 cut(s) 1340
Psp5II RGGWCCY 1 cut(s) 217
PspN4I GGNNCC 1 cut(s) 1019
PspPI GGNCC 2 cut(s) 217, 1017
PspPPI RGGWCCY 1 cut(s) 217
PsrI GAACNNNNNNTAC 2 cut(s) 1037, 1069
PstI CTGCAG 2 cut(s) 580, 949
PstNI CAGNNNCTG 1 cut(s) 953
RsaI GTAC 9 cut(s) 85, 172, 311, 326, 564, 893, 951, 1055, 1207
RsaNI GTAC 9 cut(s) 84, 171, 310, 325, 563, 892, 950, 1054, 1206
RseI CAYNNNNRTG 3 cut(s) 278, 476, 488
SacI GAGCTC 1 cut(s) 1340
SaqAI TTAA 5 cut(s) 44, 375, 932, 975, 1193
SatI GCNGC 3 cut(s) 549, 576, 1288
Sau3AI GATC 4 cut(s) 76, 430, 749, 1326
Sau96I GGNCC 2 cut(s) 217, 1017
ScaI AGTACT 1 cut(s) 564
SchI GAGTC 2 cut(s) 439, 640
SduI GDGCHC 1 cut(s) 1340
SfaNI GCATC 1 cut(s) 133
SfcI CTRYAG 4 cut(s) 576, 671, 786, 945
SinI GGWCC 1 cut(s) 217
SmiMI CAYNNNNRTG 3 cut(s) 278, 476, 488
SmlI CTYRAG 3 cut(s) 14, 43, 1090
SmoI CTYRAG 3 cut(s) 14, 43, 1090
Sse9I AATT 4 cut(s) 372, 417, 1243, 1263
SsiI CCGC 3 cut(s) 38, 1285, 1288
SspMI CTAG 2 cut(s) 1077, 1185
SstI GAGCTC 1 cut(s) 1340
StyI CCWWGG 1 cut(s) 1272
TaaI ACNGT 5 cut(s) 22, 175, 891, 1255, 1415
TaiI ACGT 1 cut(s) 987
TaqI TCGA 6 cut(s) 725, 760, 772, 886, 941, 1340
TasI AATT 4 cut(s) 372, 417, 1243, 1263
TatI WGTACW 5 cut(s) 324, 562, 891, 1053, 1205
TauI GCSGC 1 cut(s) 1290
TfiI GAWTC 3 cut(s) 210, 266, 806
Tru1I TTAA 5 cut(s) 44, 375, 932, 975, 1193
Tru9I TTAA 5 cut(s) 44, 375, 932, 975, 1193
TscAI CASTG 1 cut(s) 1420
TseFI GTSAC 5 cut(s) 346, 821, 1072, 1238, 1367
TseI GCWGC 2 cut(s) 548, 575
Tsp45I GTSAC 5 cut(s) 346, 821, 1072, 1238, 1367
TspDTI ATGAA 2 cut(s) 161, 232
TspGWI ACGGA 3 cut(s) 1171, 1226, 1298
TspRI CASTG 1 cut(s) 1420
Vha464I CTTAAG 1 cut(s) 43
VpaK11BI GGWCC 1 cut(s) 217
VspI ATTAAT 1 cut(s) 375
XapI RAATTY 1 cut(s) 417
XspI CTAG 2 cut(s) 1077, 1185
ZrmI AGTACT 1 cut(s) 564
Zsp2I ATGCAT 1 cut(s) 1152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.