RchiOBHm_Chr2g0160141

SPX and EXS domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0003754
Physical Location & Seq
Reverse (-)
76088428 .. 76094924
6497 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ52862

Sequence Viewer

Length: 1407 bp
ATGTTTGGAGGTCTTGAGACAGTTCCTTCCAATAGTCCGCACTTAAGAAATTATAGAAAAAATCTTTCAGGTACAAGTGAAGTTGAGAATGGTGCTGAGGAGGGTTTTGTGCATCTAATAGAGGGAAACGATATGAAGGGAAGCACACCATTGAGTACGGTTGCAATAATGCCGTCTCCGACTTTGCTGTGGAGATTCAAGGTCCTGCTGTTCTTCGCTTGGGGTTTCATTTGTTGCAAGATTGGATGGGATTCTGTCATGAGAATGAGTGTGGACTTGCGAGATTTATTTCTGTACGAAGCATTTTTGTACTACAATCCTCTTCTTCTTGTGACTATGATGGTTTGGTTTTGGGGAATTAATCTATGGGTGTTCGCGCAGTCCCAAGTCAGTTATCCTAAAATTTTTGAGCTTGATCAAAATCACTTGACTCACAGAGAAATATGGAAGTGTGCCACATGGATGACCATTGTTGTGCCAACAAGCATGACGGCGTATCTTTATCTCTACTCACATGGAGAAGTGTCGCTGGCTGCATCACAACCAGTACTATTATATGCTGCAGTTGCAATGATTTTGATATTCCCTTTTGATATTTTCTATCTGTCAACTCGCTTTTACTTTTTGAGGACTCTTTGGCGTATCGTTCTCCCTCTACAGGCAATATCCTTTTCTGACTTTTTCGTGGCCGATATTCTTACCTCCATGTCGAAGGTGCTTTCAGATTTGGAGCGATCAGTATGTCGAATGCTCCATCGACAGGTTGCCACTATAGCATGGTTTGAAGCTGATTCAGTTTGTGGCAGTCACTCTGTTGCAATCCCTTTGGTTCTTGTGTTGCCGTATCTTTTTCGTTTGTTCCAATGCCTTCGACAGTACAAGGATACTGGAGAGAGAACATCCCTTTTGAATGCTTTAAAGTATTCGACTGCAGTACCTGTGATTTTTCTCTCGGCCCTTAAATATCACATCTTCCCTGAAAAGTGGACAAACTTCTATCGGCCCCTTTGGCTTCTCTCGGCCATTTTGAACTCTTTGTACTCATTTTACTGGGATGTGACTAGAGATTGGGACTTGAGTGGCTTCACTCGGATTTTCAAGTCCAACAAATCACATATCTTGTCAAACCTCATACATGGACGGAAATGGGTTTATTTCTGGGTGATAACTAGCAACTTAATACTGCGTTGTACATGGACATACAAGCTGTCTGCCCATCTCCGTCACAATTATCTTACTGTGTTTGCAATTACTGCCTTGGAGATTTTCCGCCGCTTTCAATGGGTTTTCTTCCGTGTGGAAAAGGAGTGGATCAAGATGAGCTCGAAGTCTAACATTCAACTTTCCATGAGTGACATCCCAAATGAGGAAGACAGATTACTTGTTTCTAATGGCCACAGTGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EXS

Protein Analysis

468

Amino Acids

54.74

Weight (kDa)

9.0

Isoelectric Point (pI)

39.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EXS PF03124 102 - 438 2.7e-94 EXS family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 377
AciI CCGC 3 cut(s) 38, 1270, 1273
AclWI GGATC 1 cut(s) 1319
AcoI YGGCCR 3 cut(s) 687, 1020, 1393
AcsI RAATTY 1 cut(s) 402
AfaI GTAC 9 cut(s) 73, 157, 296, 311, 549, 878, 936, 1040, 1192
AfiI CCNNNNNNNGG 3 cut(s) 658, 760, 1366
AflII CTTAAG 1 cut(s) 43
AgsI TTSAA 7 cut(s) 199, 785, 910, 1030, 1099, 1280, 1340
AluBI AGCT 4 cut(s) 412, 788, 1207, 1323
AluI AGCT 4 cut(s) 412, 788, 1207, 1323
Alw21I GWGCWC 1 cut(s) 1325
Alw26I GTCTC 2 cut(s) 11, 180
AlwI GGATC 1 cut(s) 1319
AlwNI CAGNNNCTG 1 cut(s) 938
AoxI GGCC 5 cut(s) 687, 954, 1001, 1020, 1393
ApeKI GCWGC 2 cut(s) 533, 560
ApoI RAATTY 1 cut(s) 402
AseI ATTAAT 1 cut(s) 360
AspLEI GCGC 1 cut(s) 379
AspS9I GGNCC 3 cut(s) 202, 955, 1002
AsuHPI GGTGA 1 cut(s) 1174
AvaII GGWCC 1 cut(s) 202
BaeI ACNNNNGTAYC 2 cut(s) 918, 951
BalI TGGCCA 1 cut(s) 1395
BanII GRGCYC 1 cut(s) 1325
BarI GAAGNNNNNNTAC 2 cut(s) 1362, 1394
BbsI GAAGAC 1 cut(s) 1377
Bbv12I GWGCWC 1 cut(s) 1325
BbvCI CCTCAGC 1 cut(s) 96
BbvI GCAGC 2 cut(s) 520, 547
BccI CCATC 4 cut(s) 240, 334, 762, 1224
BceAI ACGGC 3 cut(s) 157, 507, 826
BciVI GTATCC 1 cut(s) 877
BclI TGATCA 1 cut(s) 415
BcoDI GTCTC 2 cut(s) 11, 180
BfaI CTAG 2 cut(s) 1062, 1170
BfmI CTRYAG 4 cut(s) 561, 656, 771, 930
BfrI CTTAAG 1 cut(s) 43
BfuI GTATCC 1 cut(s) 877
BglI GCCNNNNNGGC 1 cut(s) 1009
BisI GCNGC 3 cut(s) 534, 561, 1273
BlsI GCNGC 3 cut(s) 535, 562, 1274
BmcAI AGTACT 1 cut(s) 549
Bme18I GGWCC 1 cut(s) 202
BmgT120I GGNCC 3 cut(s) 202, 955, 1002
BmiI GGNNCC 1 cut(s) 1004
BmrI ACTGGG 1 cut(s) 1060
BmsI GCATC 2 cut(s) 121, 545
BmuI ACTGGG 1 cut(s) 1060
BpiI GAAGAC 1 cut(s) 1377
BpmI CTGGAG 1 cut(s) 909
Bpu10I CCTNAGC 1 cut(s) 96
BpuEI CTTGAG 2 cut(s) 35, 1096
BsaBI GATNNNNATC 1 cut(s) 420
BsaJI CCNNGG 1 cut(s) 1257
Bsc4I CCNNNNNNNGG 3 cut(s) 658, 760, 1366
Bse1I ACTGG 3 cut(s) 545, 892, 1055
Bse3DI GCAATG 1 cut(s) 576
Bse8I GATNNNNATC 1 cut(s) 420
BseDI CCNNGG 1 cut(s) 1257
BseGI GGATG 5 cut(s) 251, 468, 899, 1060, 1356
BseJI GATNNNNATC 1 cut(s) 420
BseLI CCNNNNNNNGG 3 cut(s) 658, 760, 1366
BseMI GCAATG 1 cut(s) 576
BseMII CTCAG 1 cut(s) 87
BseNI ACTGG 3 cut(s) 545, 892, 1055
BseRI GAGGAG 1 cut(s) 113
BseXI GCAGC 2 cut(s) 520, 547
Bsh1236I CGCG 1 cut(s) 377
BshFI GGCC 5 cut(s) 689, 956, 1003, 1022, 1395
BsiHKAI GWGCWC 1 cut(s) 1325
BslFI GGGAC 2 cut(s) 367, 1085
BslI CCNNNNNNNGG 3 cut(s) 658, 760, 1366
BsmAI GTCTC 2 cut(s) 11, 180
BsmBI CGTCTC 1 cut(s) 180
BsmFI GGGAC 2 cut(s) 367, 1085
BsmI GAATGC 2 cut(s) 753, 916
BsnI GGCC 5 cut(s) 689, 956, 1003, 1022, 1395
Bsp1286I GDGCHC 1 cut(s) 1325
Bsp1407I TGTACA 1 cut(s) 1190
Bsp143I GATC 3 cut(s) 415, 734, 1311
BspACI CCGC 3 cut(s) 38, 1270, 1273
BspANI GGCC 5 cut(s) 689, 956, 1003, 1022, 1395
BspCNI CTCAG 1 cut(s) 88
BspFNI CGCG 1 cut(s) 377
BspHI TCATGA 1 cut(s) 258
BspLI GGNNCC 1 cut(s) 1004
BspMAI CTGCAG 2 cut(s) 565, 934
BspPI GGATC 1 cut(s) 1319
BspTI CTTAAG 1 cut(s) 43
BsrDI GCAATG 1 cut(s) 576
BsrGI TGTACA 1 cut(s) 1190
BsrI ACTGG 3 cut(s) 545, 892, 1055
BssECI CCNNGG 1 cut(s) 1257
BssMI GATC 3 cut(s) 415, 734, 1311
BssT1I CCWWGG 1 cut(s) 1257
Bst4CI ACNGT 5 cut(s) 22, 160, 876, 1240, 1400
Bst6I CTCTTC 1 cut(s) 327
BstAFI CTTAAG 1 cut(s) 43
BstAPI GCANNNNNTGC 1 cut(s) 1253
BstAUI TGTACA 1 cut(s) 1190
BstC8I GCNNGC 1 cut(s) 531
BstDEI CTNAG 1 cut(s) 96
BstF5I GGATG 5 cut(s) 251, 468, 899, 1060, 1356
BstFNI CGCG 1 cut(s) 377
BstHHI GCGC 1 cut(s) 379
BstKTI GATC 3 cut(s) 418, 737, 1314
BstMAI GTCTC 2 cut(s) 11, 180
BstMBI GATC 3 cut(s) 415, 734, 1311
BstMWI GCNNNNNNNGC 4 cut(s) 566, 773, 1009, 1253
BstSFI CTRYAG 4 cut(s) 561, 656, 771, 930
BstUI CGCG 1 cut(s) 377
BstV1I GCAGC 2 cut(s) 520, 547
BstV2I GAAGAC 1 cut(s) 1377
BsuI GTATCC 1 cut(s) 877
BsuRI GGCC 5 cut(s) 689, 956, 1003, 1022, 1395
BtsCI GGATG 5 cut(s) 251, 468, 899, 1060, 1356
BtsIMutI CAGTG 1 cut(s) 1405
Cac8I GCNNGC 1 cut(s) 531
CaiI CAGNNNCTG 1 cut(s) 938
CciI TCATGA 1 cut(s) 258
CfoI GCGC 1 cut(s) 379
Cfr13I GGNCC 3 cut(s) 202, 955, 1002
Csp6I GTAC 9 cut(s) 72, 156, 295, 310, 548, 877, 935, 1039, 1191
CviAII CATG 9 cut(s) 259, 459, 487, 515, 706, 777, 1136, 1194, 1348
CviQI GTAC 9 cut(s) 72, 156, 295, 310, 548, 877, 935, 1039, 1191
DdeI CTNAG 1 cut(s) 96
DpnI GATC 3 cut(s) 417, 736, 1313
DpnII GATC 3 cut(s) 415, 734, 1311
DraI TTTAAA 1 cut(s) 918
EaeI YGGCCR 3 cut(s) 687, 1020, 1393
Eam1104I CTCTTC 1 cut(s) 327
EarI CTCTTC 1 cut(s) 327
EciI GGCGGA 1 cut(s) 1259
Ecl136II GAGCTC 1 cut(s) 1323
Eco130I CCWWGG 1 cut(s) 1257
Eco24I GRGCYC 1 cut(s) 1325
Eco47I GGWCC 1 cut(s) 202
Eco53kI GAGCTC 1 cut(s) 1323
EcoICRI GAGCTC 1 cut(s) 1323
EcoO109I RGGNCCY 1 cut(s) 202
EcoT14I CCWWGG 1 cut(s) 1257
EcoT38I GRGCYC 1 cut(s) 1325
ErhI CCWWGG 1 cut(s) 1257
Esp3I CGTCTC 1 cut(s) 180
FaeI CATG 9 cut(s) 262, 462, 490, 518, 709, 780, 1139, 1197, 1351
FaqI GGGAC 2 cut(s) 367, 1085
FatI CATG 9 cut(s) 258, 458, 486, 514, 705, 776, 1135, 1193, 1347
FbaI TGATCA 1 cut(s) 415
Fnu4HI GCNGC 3 cut(s) 534, 561, 1273
FokI GGATG 5 cut(s) 258, 475, 886, 1067, 1343
FriOI GRGCYC 1 cut(s) 1325
Fsp4HI GCNGC 3 cut(s) 534, 561, 1273
FspBI CTAG 2 cut(s) 1062, 1170
GlaI GCGC 1 cut(s) 378
GluI GCNGC 3 cut(s) 534, 561, 1273
GsuI CTGGAG 1 cut(s) 909
HaeIII GGCC 5 cut(s) 689, 956, 1003, 1022, 1395
HhaI GCGC 1 cut(s) 379
Hin1II CATG 9 cut(s) 262, 462, 490, 518, 709, 780, 1139, 1197, 1351
Hin6I GCGC 1 cut(s) 377
HinP1I GCGC 1 cut(s) 377
HincII GTYRAC 1 cut(s) 609
HindII GTYRAC 1 cut(s) 609
HinfI GANTC 5 cut(s) 195, 251, 430, 631, 791
HphI GGTGA 1 cut(s) 1174
Hpy166II GTNNAC 3 cut(s) 274, 609, 987
Hpy188I TCNGA 4 cut(s) 180, 676, 724, 1092
Hpy188III TCNNGA 3 cut(s) 14, 259, 1315
Hpy8I GTNNAC 3 cut(s) 274, 609, 987
HpyAV CCTTC 4 cut(s) 36, 130, 706, 878
HpyCH4III ACNGT 5 cut(s) 22, 160, 876, 1240, 1400
HpyCH4V TGCA 9 cut(s) 112, 164, 237, 536, 563, 569, 818, 932, 1247
HpyF10VI GCNNNNNNNGC 4 cut(s) 566, 773, 1009, 1253
HpyF3I CTNAG 1 cut(s) 96
Hsp92II CATG 9 cut(s) 262, 462, 490, 518, 709, 780, 1139, 1197, 1351
HspAI GCGC 1 cut(s) 377
Ksp22I TGATCA 1 cut(s) 415
Kzo9I GATC 3 cut(s) 415, 734, 1311
LmnI GCTCC 2 cut(s) 730, 756
Lsp1109I GCAGC 2 cut(s) 520, 547
LweI GCATC 2 cut(s) 121, 545
MaeI CTAG 2 cut(s) 1062, 1170
MaeIII GTNAC 5 cut(s) 331, 806, 1057, 1223, 1352
MalI GATC 3 cut(s) 417, 736, 1313
MboI GATC 3 cut(s) 415, 734, 1311
MboII GAAGA 6 cut(s) 205, 314, 317, 964, 1282, 1382
MhlI GDGCHC 1 cut(s) 1325
MlsI TGGCCA 1 cut(s) 1395
MluCI AATT 5 cut(s) 49, 357, 402, 1228, 1248
MluNI TGGCCA 1 cut(s) 1395
MlyI GAGTC 2 cut(s) 424, 625
MmeI TCCRAC 2 cut(s) 203, 1128
MnlI CCTC 9 cut(s) 91, 94, 115, 330, 621, 663, 712, 1139, 1360
Mox20I TGGCCA 1 cut(s) 1395
MscI TGGCCA 1 cut(s) 1395
MseI TTAA 5 cut(s) 44, 360, 917, 960, 1178
MslI CAYNNNNRTG 3 cut(s) 263, 461, 473
Msp20I TGGCCA 1 cut(s) 1395
MspCI CTTAAG 1 cut(s) 43
Mva1269I GAATGC 2 cut(s) 753, 916
MvnI CGCG 1 cut(s) 377
MwoI GCNNNNNNNGC 4 cut(s) 566, 773, 1009, 1253
NdeII GATC 3 cut(s) 415, 734, 1311
NlaIII CATG 9 cut(s) 262, 462, 490, 518, 709, 780, 1139, 1197, 1351
NlaIV GGNNCC 1 cut(s) 1004
NmeAIII GCCGAG 2 cut(s) 932, 998
NmuCI GTSAC 5 cut(s) 331, 806, 1057, 1223, 1352
PagI TCATGA 1 cut(s) 258
PctI GAATGC 2 cut(s) 753, 916
PfeI GAWTC 3 cut(s) 195, 251, 791
PkrI GCNGC 3 cut(s) 535, 562, 1274
PleI GAGTC 2 cut(s) 424, 625
PpsI GAGTC 2 cut(s) 424, 625
PpuMI RGGWCCY 1 cut(s) 202
PshBI ATTAAT 1 cut(s) 360
Psp124BI GAGCTC 1 cut(s) 1325
Psp5II RGGWCCY 1 cut(s) 202
PspN4I GGNNCC 1 cut(s) 1004
PspPI GGNCC 3 cut(s) 202, 955, 1002
PspPPI RGGWCCY 1 cut(s) 202
PsrI GAACNNNNNNTAC 2 cut(s) 1022, 1054
PstI CTGCAG 2 cut(s) 565, 934
PstNI CAGNNNCTG 1 cut(s) 938
RsaI GTAC 9 cut(s) 73, 157, 296, 311, 549, 878, 936, 1040, 1192
RsaNI GTAC 9 cut(s) 72, 156, 295, 310, 548, 877, 935, 1039, 1191
RseI CAYNNNNRTG 3 cut(s) 263, 461, 473
SacI GAGCTC 1 cut(s) 1325
SaqAI TTAA 5 cut(s) 44, 360, 917, 960, 1178
SatI GCNGC 3 cut(s) 534, 561, 1273
Sau3AI GATC 3 cut(s) 415, 734, 1311
Sau96I GGNCC 3 cut(s) 202, 955, 1002
ScaI AGTACT 1 cut(s) 549
SchI GAGTC 2 cut(s) 424, 625
SduI GDGCHC 1 cut(s) 1325
SfaNI GCATC 2 cut(s) 121, 545
SfcI CTRYAG 4 cut(s) 561, 656, 771, 930
SinI GGWCC 1 cut(s) 202
SmiMI CAYNNNNRTG 3 cut(s) 263, 461, 473
SmlI CTYRAG 3 cut(s) 14, 43, 1075
SmoI CTYRAG 3 cut(s) 14, 43, 1075
Sse9I AATT 5 cut(s) 49, 357, 402, 1228, 1248
SsiI CCGC 3 cut(s) 38, 1270, 1273
SspMI CTAG 2 cut(s) 1062, 1170
SstI GAGCTC 1 cut(s) 1325
StyI CCWWGG 1 cut(s) 1257
TaaI ACNGT 5 cut(s) 22, 160, 876, 1240, 1400
TaqI TCGA 6 cut(s) 710, 745, 757, 871, 926, 1325
TasI AATT 5 cut(s) 49, 357, 402, 1228, 1248
TatI WGTACW 5 cut(s) 309, 547, 876, 1038, 1190
TauI GCSGC 1 cut(s) 1275
TfiI GAWTC 3 cut(s) 195, 251, 791
Tru1I TTAA 5 cut(s) 44, 360, 917, 960, 1178
Tru9I TTAA 5 cut(s) 44, 360, 917, 960, 1178
TscAI CASTG 1 cut(s) 1405
TseFI GTSAC 5 cut(s) 331, 806, 1057, 1223, 1352
TseI GCWGC 2 cut(s) 533, 560
Tsp45I GTSAC 5 cut(s) 331, 806, 1057, 1223, 1352
TspDTI ATGAA 2 cut(s) 149, 217
TspGWI ACGGA 3 cut(s) 1156, 1211, 1283
TspRI CASTG 1 cut(s) 1405
Vha464I CTTAAG 1 cut(s) 43
VpaK11BI GGWCC 1 cut(s) 202
VspI ATTAAT 1 cut(s) 360
XapI RAATTY 1 cut(s) 402
XspI CTAG 2 cut(s) 1062, 1170
ZrmI AGTACT 1 cut(s) 549
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.