MD09G1104800.v1.1

SPX and EXS domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0003754
Physical Location & Seq
Forward (+)
7644596 .. 7649703
5108 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1104800.v1.1.491

Sequence Viewer

Length: 1431 bp
ATGTTTGGAGGTGTTGCGACAGTGCCTTCCAATAGTCCTCACTTAAGAAAGTCGGGTAGCAGACCTGTTGTCTCTGATCTGGGTACAAGTGAGCTTGAAAATGGTGCAGAGGAGGGTTTCTTGCGTCTGATAGAGGGAAACGATATGAAGGGTGGAAGCATGCCAAGCATACCAAGGAGTACTGCTGCAATAATGCCATCTCCAGCTATGTTATGGAGATTTAAGGTCCTACTGTTCTTTGCTTGGGGTTTCATTTGTTGCAAGGTTGGATGGGATTCTGTCATGAGGATGAGTGCGGATCTGCGAGATTTATTTCTATATGAGGCATTTTTGTATTATAATCCTCTACTTCTTGTGACTATGATGGTTTGGTTTTGGGGAGTCAATCTATGGGTTTTTGCTCAAGGCAATGTAAACTATGCCAAAATTTTTGATCTTGATCAAAATCATTTGACCCACAGAGAAATATGGAAGTGTGCGACATGGATGACAATTGTTGTGCCAACAAGCATGACAGCTTATCTTTATCTCTATTCGCATGGAGAAGTGTCACTTGCTGCATCACAACCAGTACTATTATATGCTGCTGTTGCAATGCTTTTGATATTCCCTTTTGACATTTTCTATTTGTCATCTCGCTTTTACTTGTTAAGGACTCTTTGGCGTATAGTTCTCCCCTTACAGGCAATATCATTTTCTGACTTTTTCCTGGCTGATATTTTGACCTCCATGTCTAAGGTGCTTTCAGATTTGGAGCGATCAGTATGTCGAATGGTCCATCGACAGGTTGCCACTATTGCATGGTTTGAAGCTGACTCAGTTTGTGGAAGTCACTCTGTTGCAATCCCTGTGGTTCTTGTTTTGCCTTACCTTTTTCGTTTGTTTCAATGCCTTCGACAATATAAGGATACTGGGGAGAAAACAACCCTTCTAAATGCTTTGAAGTATTCTACTGCAGTACCTGTGATTTTTCTTTCAGCCCTCAAATATCATGTCTTCCCTGAAAAGTGGACGACCTTTTATCGGCCCCTCTGGCTTCTCTCAGGTGTTTTGAACTCTTTGTACTCGTTCTACTGGGACGTGACTAGAGATTGGGACTTAAGTGGTTTCACTCGGATTTTCAAGATCAGCAAGCAACAATTCTTGTCAAACCTCATACATGGACGGAAATGGGTTTACTTCTGGGTGATAGGTAGCAACCTGATTCTGCGTTGTACCTGGACATACAAGCTCTCTTCCCATCTCCGCCACAATTACCTTACCGTATTCGCAATCACTGCCTTGGAGATTTTCCGCCGCTTCCAATGGGTTTTCTTCCGCGTAGAAAACGAGGTGAACAAGATGAGCTCTAAATCAAACATTCAGCTTTCCATGGCCGACAATCCAAATGAGGAAGACAGTTTACTCGTCTCTAATGGCCACAGCGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EXS

Protein Analysis

477

Amino Acids

54.96

Weight (kDa)

9.0

Isoelectric Point (pI)

43.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EXS PF03124 110 - 445 1.6e-94 EXS family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 339
AasI GACNNNNNNGTC 1 cut(s) 730
AccII CGCG 1 cut(s) 1320
AciI CCGC 5 cut(s) 296, 1246, 1294, 1297, 1318
AclWI GGATC 1 cut(s) 306
AcoI YGGCCR 2 cut(s) 1374, 1417
AcsI RAATTY 1 cut(s) 426
AfaI GTAC 6 cut(s) 85, 181, 573, 960, 1064, 1216
AfiI CCNNNNNNNGG 3 cut(s) 682, 784, 1023
AflII CTTAAG 2 cut(s) 43, 1099
AgsI TTSAA 6 cut(s) 98, 809, 887, 943, 1054, 1123
AhdI GACNNNNNGTC 1 cut(s) 68
AjiI CACGTC 1 cut(s) 1081
AjnI CCWGG 2 cut(s) 708, 1217
AluBI AGCT 7 cut(s) 94, 206, 518, 812, 1231, 1347, 1366
AluI AGCT 7 cut(s) 94, 206, 518, 812, 1231, 1347, 1366
Alw21I GWGCWC 1 cut(s) 1349
Alw26I GTCTC 2 cut(s) 76, 1414
AlwI GGATC 1 cut(s) 306
AlwNI CAGNNNCTG 1 cut(s) 962
AoxI GGCC 3 cut(s) 1025, 1374, 1417
ApeKI GCWGC 3 cut(s) 185, 557, 584
ApoI RAATTY 1 cut(s) 426
ArsI GACNNNNNNTTYG 2 cut(s) 978, 1010
AspS9I GGNCC 3 cut(s) 226, 775, 1026
AsuHPI GGTGA 2 cut(s) 1198, 1345
AvaII GGWCC 2 cut(s) 226, 775
BaeI ACNNNNGTAYC 2 cut(s) 942, 975
BalI TGGCCA 1 cut(s) 1419
BanII GRGCYC 1 cut(s) 1349
BarI GAAGNNNNNNTAC 2 cut(s) 1386, 1418
BbsI GAAGAC 2 cut(s) 988, 1401
Bbv12I GWGCWC 1 cut(s) 1349
BbvI GCAGC 3 cut(s) 172, 544, 571
BccI CCATC 5 cut(s) 205, 264, 358, 786, 1248
BciT130I CCWGG 2 cut(s) 710, 1219
BciVI GTATCC 1 cut(s) 901
BclI TGATCA 1 cut(s) 439
BcoDI GTCTC 2 cut(s) 76, 1414
BfaI CTAG 1 cut(s) 1086
BfmI CTRYAG 1 cut(s) 954
BfrI CTTAAG 2 cut(s) 43, 1099
BfuI GTATCC 1 cut(s) 901
BglI GCCNNNNNGGC 1 cut(s) 1033
BisI GCNGC 4 cut(s) 186, 558, 585, 1297
BlsI GCNGC 4 cut(s) 187, 559, 586, 1298
BmcAI AGTACT 2 cut(s) 181, 573
Bme1390I CCNGG 2 cut(s) 710, 1219
Bme18I GGWCC 2 cut(s) 226, 775
BmeRI GACNNNNNGTC 1 cut(s) 68
BmgBI CACGTC 1 cut(s) 1081
BmgT120I GGNCC 3 cut(s) 226, 775, 1026
BmiI GGNNCC 1 cut(s) 1028
BmrFI CCNGG 2 cut(s) 710, 1219
BmrI ACTGGG 2 cut(s) 921, 1084
BmsI GCATC 1 cut(s) 569
BmuI ACTGGG 2 cut(s) 921, 1084
BpiI GAAGAC 2 cut(s) 988, 1401
BpmI CTGGAG 1 cut(s) 186
BpuEI CTTGAG 1 cut(s) 387
BsaBI GATNNNNATC 2 cut(s) 438, 444
BsaJI CCNNGG 3 cut(s) 173, 1281, 1371
Bsc4I CCNNNNNNNGG 3 cut(s) 682, 784, 1023
Bse1I ACTGG 3 cut(s) 569, 916, 1079
Bse3DI GCAATG 2 cut(s) 415, 600
Bse8I GATNNNNATC 2 cut(s) 438, 444
BseBI CCWGG 2 cut(s) 710, 1219
BseDI CCNNGG 3 cut(s) 173, 1281, 1371
BseGI GGATG 3 cut(s) 275, 294, 492
BseJI GATNNNNATC 2 cut(s) 438, 444
BseLI CCNNNNNNNGG 3 cut(s) 682, 784, 1023
BseMI GCAATG 2 cut(s) 415, 600
BseMII CTCAG 2 cut(s) 831, 1056
BseNI ACTGG 3 cut(s) 569, 916, 1079
BseRI GAGGAG 1 cut(s) 125
BseXI GCAGC 3 cut(s) 172, 544, 571
BsgI GTGCAG 1 cut(s) 126
Bsh1236I CGCG 1 cut(s) 1320
BshFI GGCC 3 cut(s) 1027, 1376, 1419
BsiHKAI GWGCWC 1 cut(s) 1349
BslFI GGGAC 2 cut(s) 1091, 1109
BslI CCNNNNNNNGG 3 cut(s) 682, 784, 1023
BsmAI GTCTC 2 cut(s) 76, 1414
BsmBI CGTCTC 1 cut(s) 1414
BsmFI GGGAC 2 cut(s) 1091, 1109
BsnI GGCC 3 cut(s) 1027, 1376, 1419
Bsp1286I GDGCHC 1 cut(s) 1349
Bsp143I GATC 6 cut(s) 76, 298, 433, 439, 758, 1125
Bsp19I CCATGG 1 cut(s) 1371
BspACI CCGC 5 cut(s) 296, 1246, 1294, 1297, 1318
BspANI GGCC 3 cut(s) 1027, 1376, 1419
BspCNI CTCAG 2 cut(s) 830, 1055
BspFNI CGCG 1 cut(s) 1320
BspHI TCATGA 1 cut(s) 282
BspLI GGNNCC 1 cut(s) 1028
BspMAI CTGCAG 1 cut(s) 958
BspPI GGATC 1 cut(s) 306
BspTI CTTAAG 2 cut(s) 43, 1099
BsrDI GCAATG 2 cut(s) 415, 600
BsrI ACTGG 3 cut(s) 569, 916, 1079
BssECI CCNNGG 3 cut(s) 173, 1281, 1371
BssMI GATC 6 cut(s) 76, 298, 433, 439, 758, 1125
BssT1I CCWWGG 3 cut(s) 173, 1281, 1371
Bst2UI CCWGG 2 cut(s) 710, 1219
Bst4CI ACNGT 4 cut(s) 22, 234, 1264, 1400
Bst6I CTCTTC 1 cut(s) 1240
BstAFI CTTAAG 2 cut(s) 43, 1099
BstAPI GCANNNNNTGC 1 cut(s) 1277
BstC8I GCNNGC 2 cut(s) 161, 1133
BstDEI CTNAG 3 cut(s) 735, 817, 1042
BstDSI CCRYGG 1 cut(s) 1371
BstF5I GGATG 3 cut(s) 275, 294, 492
BstFNI CGCG 1 cut(s) 1320
BstKTI GATC 6 cut(s) 79, 301, 436, 442, 761, 1128
BstMAI GTCTC 2 cut(s) 76, 1414
BstMBI GATC 6 cut(s) 76, 298, 433, 439, 758, 1125
BstMWI GCNNNNNNNGC 5 cut(s) 165, 590, 797, 1033, 1277
BstNI CCWGG 2 cut(s) 710, 1219
BstNSI RCATGY 1 cut(s) 163
BstSCI CCNGG 2 cut(s) 708, 1217
BstSFI CTRYAG 1 cut(s) 954
BstUI CGCG 1 cut(s) 1320
BstV1I GCAGC 3 cut(s) 172, 544, 571
BstV2I GAAGAC 2 cut(s) 988, 1401
BstX2I RGATCY 1 cut(s) 298
BstYI RGATCY 1 cut(s) 298
BsuI GTATCC 1 cut(s) 901
BsuRI GGCC 3 cut(s) 1027, 1376, 1419
BtgI CCRYGG 1 cut(s) 1371
BtrI CACGTC 1 cut(s) 1081
BtsCI GGATG 3 cut(s) 275, 294, 492
BtsI GCAGTG 1 cut(s) 1275
BtsIMutI CAGTG 2 cut(s) 27, 1275
Cac8I GCNNGC 2 cut(s) 161, 1133
CaiI CAGNNNCTG 1 cut(s) 962
CciI TCATGA 1 cut(s) 282
Cfr13I GGNCC 3 cut(s) 226, 775, 1026
CseI GACGC 1 cut(s) 113
Csp6I GTAC 6 cut(s) 84, 180, 572, 959, 1063, 1215
CspCI CAANNNNNGTGG 2 cut(s) 831, 866
CviQI GTAC 6 cut(s) 84, 180, 572, 959, 1063, 1215
DdeI CTNAG 3 cut(s) 735, 817, 1042
DpnI GATC 6 cut(s) 78, 300, 435, 441, 760, 1127
DpnII GATC 6 cut(s) 76, 298, 433, 439, 758, 1125
DrdI GACNNNNNNGTC 1 cut(s) 730
DriI GACNNNNNGTC 1 cut(s) 68
DseDI GACNNNNNNGTC 1 cut(s) 730
EaeI YGGCCR 2 cut(s) 1374, 1417
Eam1104I CTCTTC 1 cut(s) 1240
Eam1105I GACNNNNNGTC 1 cut(s) 68
EarI CTCTTC 1 cut(s) 1240
EciI GGCGGA 2 cut(s) 1235, 1283
Ecl136II GAGCTC 1 cut(s) 1347
Eco130I CCWWGG 3 cut(s) 173, 1281, 1371
Eco24I GRGCYC 1 cut(s) 1349
Eco47I GGWCC 2 cut(s) 226, 775
Eco53kI GAGCTC 1 cut(s) 1347
EcoICRI GAGCTC 1 cut(s) 1347
EcoO109I RGGNCCY 1 cut(s) 226
EcoRII CCWGG 2 cut(s) 708, 1217
EcoT14I CCWWGG 3 cut(s) 173, 1281, 1371
EcoT38I GRGCYC 1 cut(s) 1349
ErhI CCWWGG 3 cut(s) 173, 1281, 1371
Esp3I CGTCTC 1 cut(s) 1414
FalI AAGNNNNNCTT 2 cut(s) 537, 569
FaqI GGGAC 2 cut(s) 1091, 1109
FbaI TGATCA 1 cut(s) 439
Fnu4HI GCNGC 4 cut(s) 186, 558, 585, 1297
FokI GGATG 3 cut(s) 282, 301, 499
FriOI GRGCYC 1 cut(s) 1349
Fsp4HI GCNGC 4 cut(s) 186, 558, 585, 1297
FspBI CTAG 1 cut(s) 1086
GluI GCNGC 4 cut(s) 186, 558, 585, 1297
GsuI CTGGAG 1 cut(s) 186
HaeIII GGCC 3 cut(s) 1027, 1376, 1419
HgaI GACGC 1 cut(s) 113
HinfI GANTC 5 cut(s) 275, 381, 655, 815, 1204
HphI GGTGA 2 cut(s) 1198, 1345
Hpy166II GTNNAC 5 cut(s) 415, 1011, 1177, 1336, 1403
Hpy188I TCNGA 5 cut(s) 76, 129, 700, 748, 1116
Hpy188III TCNNGA 3 cut(s) 283, 437, 1123
Hpy8I GTNNAC 5 cut(s) 415, 1011, 1177, 1336, 1403
HpyAV CCTTC 4 cut(s) 36, 142, 902, 938
HpyCH4III ACNGT 4 cut(s) 22, 234, 1264, 1400
HpyCH4IV ACGT 1 cut(s) 1080
HpyCH4V TGCA 8 cut(s) 107, 188, 261, 560, 593, 800, 842, 956
HpyF10VI GCNNNNNNNGC 5 cut(s) 165, 590, 797, 1033, 1277
HpyF3I CTNAG 3 cut(s) 735, 817, 1042
HpySE526I ACGT 1 cut(s) 1080
Ksp22I TGATCA 1 cut(s) 439
Kzo9I GATC 6 cut(s) 76, 298, 433, 439, 758, 1125
LmnI GCTCC 1 cut(s) 754
Lsp1109I GCAGC 3 cut(s) 172, 544, 571
LweI GCATC 1 cut(s) 569
MaeI CTAG 1 cut(s) 1086
MaeII ACGT 1 cut(s) 1080
MaeIII GTNAC 4 cut(s) 355, 549, 830, 1081
MalI GATC 6 cut(s) 78, 300, 435, 441, 760, 1127
MboI GATC 6 cut(s) 76, 298, 433, 439, 758, 1125
MboII GAAGA 4 cut(s) 988, 1227, 1306, 1406
MfeI CAATTG 1 cut(s) 492
MflI RGATCY 1 cut(s) 298
MhlI GDGCHC 1 cut(s) 1349
MlsI TGGCCA 1 cut(s) 1419
MluCI AATT 4 cut(s) 426, 492, 1139, 1252
MluNI TGGCCA 1 cut(s) 1419
MlyI GAGTC 3 cut(s) 390, 649, 809
MmeI TCCRAC 1 cut(s) 247
Mox20I TGGCCA 1 cut(s) 1419
MscI TGGCCA 1 cut(s) 1419
MseI TTAA 4 cut(s) 44, 222, 650, 1100
MslI CAYNNNNRTG 1 cut(s) 287
Msp20I TGGCCA 1 cut(s) 1419
MspCI CTTAAG 2 cut(s) 43, 1099
MspR9I CCNGG 2 cut(s) 710, 1219
MunI CAATTG 1 cut(s) 492
MvaI CCWGG 2 cut(s) 710, 1219
MvnI CGCG 1 cut(s) 1320
MwoI GCNNNNNNNGC 5 cut(s) 165, 590, 797, 1033, 1277
NcoI CCATGG 1 cut(s) 1371
NdeII GATC 6 cut(s) 76, 298, 433, 439, 758, 1125
NlaIV GGNNCC 1 cut(s) 1028
NmuCI GTSAC 4 cut(s) 355, 549, 830, 1081
NspI RCATGY 1 cut(s) 163
PaeI GCATGC 1 cut(s) 163
PagI TCATGA 1 cut(s) 282
PfeI GAWTC 2 cut(s) 275, 1204
PkrI GCNGC 4 cut(s) 187, 559, 586, 1298
PleI GAGTC 3 cut(s) 389, 649, 809
PpsI GAGTC 3 cut(s) 389, 649, 809
PpuMI RGGWCCY 1 cut(s) 226
PsiI TTATAA 1 cut(s) 339
Psp124BI GAGCTC 1 cut(s) 1349
Psp5II RGGWCCY 1 cut(s) 226
Psp6I CCWGG 2 cut(s) 708, 1217
PspGI CCWGG 2 cut(s) 708, 1217
PspN4I GGNNCC 1 cut(s) 1028
PspPI GGNCC 3 cut(s) 226, 775, 1026
PspPPI RGGWCCY 1 cut(s) 226
PsrI GAACNNNNNNTAC 2 cut(s) 1046, 1078
PstI CTGCAG 1 cut(s) 958
PstNI CAGNNNCTG 1 cut(s) 962
PsuI RGATCY 1 cut(s) 298
RsaI GTAC 6 cut(s) 85, 181, 573, 960, 1064, 1216
RsaNI GTAC 6 cut(s) 84, 180, 572, 959, 1063, 1215
RseI CAYNNNNRTG 1 cut(s) 287
SacI GAGCTC 1 cut(s) 1349
SaqAI TTAA 4 cut(s) 44, 222, 650, 1100
SatI GCNGC 4 cut(s) 186, 558, 585, 1297
Sau3AI GATC 6 cut(s) 76, 298, 433, 439, 758, 1125
Sau96I GGNCC 3 cut(s) 226, 775, 1026
ScaI AGTACT 2 cut(s) 181, 573
SchI GAGTC 3 cut(s) 390, 649, 809
ScrFI CCNGG 2 cut(s) 710, 1219
SduI GDGCHC 1 cut(s) 1349
SfaNI GCATC 1 cut(s) 569
SfcI CTRYAG 1 cut(s) 954
SinI GGWCC 2 cut(s) 226, 775
SmiMI CAYNNNNRTG 1 cut(s) 287
SmlI CTYRAG 3 cut(s) 43, 402, 1099
SmoI CTYRAG 3 cut(s) 43, 402, 1099
SphI GCATGC 1 cut(s) 163
Sse9I AATT 4 cut(s) 426, 492, 1139, 1252
SsiI CCGC 5 cut(s) 296, 1246, 1294, 1297, 1318
SspMI CTAG 1 cut(s) 1086
SstI GAGCTC 1 cut(s) 1349
StyD4I CCNGG 2 cut(s) 708, 1217
StyI CCWWGG 3 cut(s) 173, 1281, 1371
TaaI ACNGT 4 cut(s) 22, 234, 1264, 1400
TaiI ACGT 1 cut(s) 1083
TaqI TCGA 3 cut(s) 769, 781, 895
TasI AATT 4 cut(s) 426, 492, 1139, 1252
TatI WGTACW 3 cut(s) 179, 571, 1062
TauI GCSGC 1 cut(s) 1299
TfiI GAWTC 2 cut(s) 275, 1204
Tru1I TTAA 4 cut(s) 44, 222, 650, 1100
Tru9I TTAA 4 cut(s) 44, 222, 650, 1100
TscAI CASTG 2 cut(s) 27, 1282
TseFI GTSAC 4 cut(s) 355, 549, 830, 1081
TseI GCWGC 3 cut(s) 185, 557, 584
Tsp45I GTSAC 4 cut(s) 355, 549, 830, 1081
TspDTI ATGAA 2 cut(s) 161, 241
TspGWI ACGGA 1 cut(s) 1180
TspRI CASTG 2 cut(s) 27, 1282
Vha464I CTTAAG 2 cut(s) 43, 1099
VpaK11BI GGWCC 2 cut(s) 226, 775
XapI RAATTY 1 cut(s) 426
XceI RCATGY 1 cut(s) 163
XcmI CCANNNNNNNNNTGG 1 cut(s) 210
XspI CTAG 1 cut(s) 1086
ZrmI AGTACT 2 cut(s) 181, 573
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.