MD17G1092500.v1.1

SPX and EXS domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0003754
Physical Location & Seq
Forward (+)
7770306 .. 7775827
5522 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1092500.v1.1.491

Sequence Viewer

Length: 1431 bp
ATGTTTGGAGGTGTTGCGACAGTGCCTTCCAATAGTCCTCACTTAAGAAAGTCGGGTAGCAGACCTGTTGTCTCTGATCTGGGTACAAGTGAGCTCGAAAATGATGCACAGGAGGGCTTCTTGCGTCTGATAGAGGGAAACGATAGGAAGGGAGGAAACATGCCAAGCATACCAATGAGTACTGCTGCAATAATGCCATCTCCAGCTATGTTATGGAGATTTAAGGTCCTACTGTTCTGTGTATGGGGTTTCATTTGTTGCAAGGTTGGATGGGATTCTGTGATGAGGATGAGTGTGGATCTGCGAGATTTATTTCTATACGAGGCATTTTTGTATTATAATCCTCTACTTCTTGTGACTATGATGGTTTGGTTTTGGGGAGTCAATCTATGGGTTTTTGCTCAGGGCAATGTAAACTATGCCAAAATTTTTGATCTTGATCAAAATCATTTGACCCACAGAGAAATATGGAAGTGTGCGACCTGGATGACAATTGTTGTGCCGACAAGCTTGACAGCATATCTTTATCTCTATTCGCATGGAGAAGTGTCGCTTGCTGCATCACAACCAGTACTATTGTATGCTGCTGTTGCAATGCTTTTGATATTCCCTTTTGACATTTTCTATTTGTCATCTCGCTTTTACTTGTTAAGGACTCTATGGCGTATAGTTCTCCCACTACAGGCAATATCATTTTCTGACTTTTTCCTGGCTGATATTTTGACCTCCATGTCTAAGGTGCTTTCAGATTTGGAGCGATCAGTATGTCGAATGGTCCATCGACAGGTTGCCACTATTGCATGGTTTGAAGCTGACTCAGTTTGTGGAAGTCATTCTGTTGCAATCCCTGTAGTTCTTGTTTTGCCTTACCTTTTTCGTTTGTTCCAATGTCTTCGACAATATAAGGATACTGGGGAGAAAACAACCCTTCTAAATGCTTTGAAGTATTCTACCGCGGTACCTGTGATTTTTCTTTCAGCCCTCAAATATCATGTCTTCCCTGAAAAGTGGACGACCTTTTATCGGCCCCTCTGGCTTCTCTCAGGTGTTTTGAACTCCCTGTACTCGTTCTACTGGGATGTGACTAGAGATTGGGACTTAAGTGGTTTCACTCGGATTTTCAAGTTTAGCAAACAACATTTCTTGTCAAACGTCATACATGGACGGAAATGGGTTTACTTCTGGGTGATATTTAGCAACCTGATTCTGCGTTGTACCTGGACATACAAGCTCTCTTCCCATCTTCGCCACAATTACCTTACCGTGTTTGCAATCACTGCCCTGGAGATTTTCCGCCGCTTCCAATGGGTTTTCTTCCGTGTAGAAAACGAGGTGAACAAGATGAGTTCGAAGTCAAACATTCAGCTTTCCATGATAGACAACCCAAATGAGGAAGACAGATTACTCGTCTCTAATGGCCACAGCGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EXS

Protein Analysis

477

Amino Acids

55.27

Weight (kDa)

9.04

Isoelectric Point (pI)

41.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EXS PF03124 110 - 445 2.6e-94 EXS family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 339
AasI GACNNNNNNGTC 1 cut(s) 730
Acc65I GGTACC 1 cut(s) 958
AccB1I GGYRCC 1 cut(s) 958
AccII CGCG 1 cut(s) 956
AciI CCGC 4 cut(s) 954, 956, 1294, 1297
AclWI GGATC 1 cut(s) 306
AcoI YGGCCR 1 cut(s) 1417
AcsI RAATTY 1 cut(s) 426
AdeI CACNNNGTG 1 cut(s) 1426
AfaI GTAC 6 cut(s) 85, 181, 573, 960, 1064, 1216
AfiI CCNNNNNNNGG 4 cut(s) 682, 784, 1023, 1390
AflII CTTAAG 2 cut(s) 43, 1099
AgsI TTSAA 4 cut(s) 809, 943, 1054, 1123
AhdI GACNNNNNGTC 1 cut(s) 68
AjnI CCWGG 4 cut(s) 482, 708, 1217, 1281
AluBI AGCT 6 cut(s) 94, 206, 510, 812, 1231, 1366
AluI AGCT 6 cut(s) 94, 206, 510, 812, 1231, 1366
Alw21I GWGCWC 1 cut(s) 96
Alw26I GTCTC 2 cut(s) 76, 1414
AlwI GGATC 1 cut(s) 306
AoxI GGCC 2 cut(s) 1025, 1417
ApeKI GCWGC 3 cut(s) 185, 557, 584
ApoI RAATTY 1 cut(s) 426
ArsI GACNNNNNNTTYG 2 cut(s) 978, 1010
Asp700I GAANNNNTTC 1 cut(s) 832
Asp718I GGTACC 1 cut(s) 958
AspS9I GGNCC 3 cut(s) 226, 775, 1026
AsuHPI GGTGA 2 cut(s) 1198, 1345
AsuII TTCGAA 1 cut(s) 1349
AvaII GGWCC 2 cut(s) 226, 775
BaeI ACNNNNGTAYC 2 cut(s) 942, 975
BalI TGGCCA 1 cut(s) 1419
BanI GGYRCC 1 cut(s) 958
BanII GRGCYC 1 cut(s) 96
BarI GAAGNNNNNNTAC 2 cut(s) 1386, 1418
BbsI GAAGAC 3 cut(s) 884, 988, 1401
Bbv12I GWGCWC 1 cut(s) 96
BbvI GCAGC 3 cut(s) 172, 544, 571
BccI CCATC 5 cut(s) 205, 264, 358, 786, 1248
BcgI CGANNNNNNTGC 2 cut(s) 86, 120
BciT130I CCWGG 4 cut(s) 484, 710, 1219, 1283
BciVI GTATCC 1 cut(s) 901
BclI TGATCA 1 cut(s) 439
BcoDI GTCTC 2 cut(s) 76, 1414
BfaI CTAG 1 cut(s) 1086
BfmI CTRYAG 2 cut(s) 680, 849
BfrI CTTAAG 2 cut(s) 43, 1099
BfuI GTATCC 1 cut(s) 901
BglI GCCNNNNNGGC 1 cut(s) 1033
BisI GCNGC 4 cut(s) 186, 558, 585, 1297
BlsI GCNGC 4 cut(s) 187, 559, 586, 1298
BmcAI AGTACT 2 cut(s) 181, 573
Bme1390I CCNGG 4 cut(s) 484, 710, 1219, 1283
Bme18I GGWCC 2 cut(s) 226, 775
BmeRI GACNNNNNGTC 1 cut(s) 68
BmgT120I GGNCC 3 cut(s) 226, 775, 1026
BmiI GGNNCC 2 cut(s) 960, 1028
BmrFI CCNGG 4 cut(s) 484, 710, 1219, 1283
BmrI ACTGGG 2 cut(s) 921, 1084
BmsI GCATC 2 cut(s) 94, 569
BmuI ACTGGG 2 cut(s) 921, 1084
BpiI GAAGAC 3 cut(s) 884, 988, 1401
BpmI CTGGAG 2 cut(s) 186, 1304
Bpu10I CCTNAGC 1 cut(s) 402
Bpu14I TTCGAA 1 cut(s) 1349
BsaBI GATNNNNATC 2 cut(s) 438, 444
BsaJI CCNNGG 2 cut(s) 954, 1281
Bsc4I CCNNNNNNNGG 4 cut(s) 682, 784, 1023, 1390
Bse1I ACTGG 3 cut(s) 569, 916, 1079
Bse3DI GCAATG 2 cut(s) 415, 600
Bse8I GATNNNNATC 2 cut(s) 438, 444
BseBI CCWGG 4 cut(s) 484, 710, 1219, 1283
BseDI CCNNGG 2 cut(s) 954, 1281
BseGI GGATG 4 cut(s) 275, 294, 492, 1084
BseJI GATNNNNATC 2 cut(s) 438, 444
BseLI CCNNNNNNNGG 4 cut(s) 682, 784, 1023, 1390
BseMI GCAATG 2 cut(s) 415, 600
BseMII CTCAG 3 cut(s) 416, 831, 1056
BseNI ACTGG 3 cut(s) 569, 916, 1079
BseXI GCAGC 3 cut(s) 172, 544, 571
Bsh1236I CGCG 1 cut(s) 956
BshFI GGCC 2 cut(s) 1027, 1419
BshNI GGYRCC 1 cut(s) 958
BsiHKAI GWGCWC 1 cut(s) 96
BslFI GGGAC 1 cut(s) 1109
BslI CCNNNNNNNGG 4 cut(s) 682, 784, 1023, 1390
BsmAI GTCTC 2 cut(s) 76, 1414
BsmBI CGTCTC 1 cut(s) 1414
BsmFI GGGAC 1 cut(s) 1109
BsnI GGCC 2 cut(s) 1027, 1419
Bsp119I TTCGAA 1 cut(s) 1349
Bsp1286I GDGCHC 1 cut(s) 96
Bsp143I GATC 5 cut(s) 76, 298, 433, 439, 758
BspACI CCGC 4 cut(s) 954, 956, 1294, 1297
BspANI GGCC 2 cut(s) 1027, 1419
BspCNI CTCAG 3 cut(s) 415, 830, 1055
BspFNI CGCG 1 cut(s) 956
BspLI GGNNCC 2 cut(s) 960, 1028
BspPI GGATC 1 cut(s) 306
BspT104I TTCGAA 1 cut(s) 1349
BspT107I GGYRCC 1 cut(s) 958
BspTI CTTAAG 2 cut(s) 43, 1099
BsrDI GCAATG 2 cut(s) 415, 600
BsrI ACTGG 3 cut(s) 569, 916, 1079
BssECI CCNNGG 2 cut(s) 954, 1281
BssMI GATC 5 cut(s) 76, 298, 433, 439, 758
Bst2UI CCWGG 4 cut(s) 484, 710, 1219, 1283
Bst4CI ACNGT 3 cut(s) 22, 234, 1264
Bst6I CTCTTC 1 cut(s) 1240
BstAFI CTTAAG 2 cut(s) 43, 1099
BstAPI GCANNNNNTGC 1 cut(s) 1277
BstBI TTCGAA 1 cut(s) 1349
BstC8I GCNNGC 1 cut(s) 555
BstDEI CTNAG 4 cut(s) 402, 735, 817, 1042
BstDSI CCRYGG 1 cut(s) 954
BstF5I GGATG 4 cut(s) 275, 294, 492, 1084
BstFNI CGCG 1 cut(s) 956
BstKTI GATC 5 cut(s) 79, 301, 436, 442, 761
BstMAI GTCTC 2 cut(s) 76, 1414
BstMBI GATC 5 cut(s) 76, 298, 433, 439, 758
BstMWI GCNNNNNNNGC 4 cut(s) 590, 797, 1033, 1277
BstNI CCWGG 4 cut(s) 484, 710, 1219, 1283
BstNSI RCATGY 1 cut(s) 163
BstSCI CCNGG 4 cut(s) 482, 708, 1217, 1281
BstSFI CTRYAG 2 cut(s) 680, 849
BstUI CGCG 1 cut(s) 956
BstV1I GCAGC 3 cut(s) 172, 544, 571
BstV2I GAAGAC 3 cut(s) 884, 988, 1401
BstX2I RGATCY 1 cut(s) 298
BstYI RGATCY 1 cut(s) 298
BsuI GTATCC 1 cut(s) 901
BsuRI GGCC 2 cut(s) 1027, 1419
BtgI CCRYGG 1 cut(s) 954
BtsCI GGATG 4 cut(s) 275, 294, 492, 1084
BtsI GCAGTG 1 cut(s) 1275
BtsIMutI CAGTG 2 cut(s) 27, 1275
Cac8I GCNNGC 1 cut(s) 555
Cfr13I GGNCC 3 cut(s) 226, 775, 1026
Cfr42I CCGCGG 1 cut(s) 957
CseI GACGC 1 cut(s) 113
Csp6I GTAC 6 cut(s) 84, 180, 572, 959, 1063, 1215
CviAII CATG 7 cut(s) 160, 539, 730, 801, 992, 1160, 1372
CviQI GTAC 6 cut(s) 84, 180, 572, 959, 1063, 1215
DdeI CTNAG 4 cut(s) 402, 735, 817, 1042
DpnI GATC 5 cut(s) 78, 300, 435, 441, 760
DpnII GATC 5 cut(s) 76, 298, 433, 439, 758
DraIII CACNNNGTG 1 cut(s) 1426
DrdI GACNNNNNNGTC 1 cut(s) 730
DriI GACNNNNNGTC 1 cut(s) 68
DseDI GACNNNNNNGTC 1 cut(s) 730
EaeI YGGCCR 1 cut(s) 1417
Eam1104I CTCTTC 1 cut(s) 1240
Eam1105I GACNNNNNGTC 1 cut(s) 68
EarI CTCTTC 1 cut(s) 1240
EciI GGCGGA 1 cut(s) 1283
Ecl136II GAGCTC 1 cut(s) 94
Eco24I GRGCYC 1 cut(s) 96
Eco47I GGWCC 2 cut(s) 226, 775
Eco53kI GAGCTC 1 cut(s) 94
EcoICRI GAGCTC 1 cut(s) 94
EcoO109I RGGNCCY 1 cut(s) 226
EcoRII CCWGG 4 cut(s) 482, 708, 1217, 1281
EcoT38I GRGCYC 1 cut(s) 96
Esp3I CGTCTC 1 cut(s) 1414
FaeI CATG 7 cut(s) 163, 542, 733, 804, 995, 1163, 1375
FalI AAGNNNNNCTT 2 cut(s) 537, 569
FaqI GGGAC 1 cut(s) 1109
FatI CATG 7 cut(s) 159, 538, 729, 800, 991, 1159, 1371
FbaI TGATCA 1 cut(s) 439
Fnu4HI GCNGC 4 cut(s) 186, 558, 585, 1297
FokI GGATG 4 cut(s) 282, 301, 499, 1091
FriOI GRGCYC 1 cut(s) 96
Fsp4HI GCNGC 4 cut(s) 186, 558, 585, 1297
FspBI CTAG 1 cut(s) 1086
GluI GCNGC 4 cut(s) 186, 558, 585, 1297
GsuI CTGGAG 2 cut(s) 186, 1304
HaeIII GGCC 2 cut(s) 1027, 1419
HgaI GACGC 1 cut(s) 113
Hin1II CATG 7 cut(s) 163, 542, 733, 804, 995, 1163, 1375
HindIII AAGCTT 1 cut(s) 508
HinfI GANTC 5 cut(s) 275, 381, 655, 815, 1204
HphI GGTGA 2 cut(s) 1198, 1345
Hpy166II GTNNAC 4 cut(s) 415, 1011, 1177, 1336
Hpy188I TCNGA 5 cut(s) 76, 129, 700, 748, 1116
Hpy188III TCNNGA 1 cut(s) 437
Hpy8I GTNNAC 4 cut(s) 415, 1011, 1177, 1336
HpyAV CCTTC 3 cut(s) 36, 142, 938
HpyCH4III ACNGT 3 cut(s) 22, 234, 1264
HpyCH4IV ACGT 1 cut(s) 1152
HpyCH4V TGCA 8 cut(s) 107, 188, 261, 560, 593, 800, 842, 1271
HpyF10VI GCNNNNNNNGC 4 cut(s) 590, 797, 1033, 1277
HpyF3I CTNAG 4 cut(s) 402, 735, 817, 1042
HpySE526I ACGT 1 cut(s) 1152
Hsp92II CATG 7 cut(s) 163, 542, 733, 804, 995, 1163, 1375
KpnI GGTACC 1 cut(s) 962
Ksp22I TGATCA 1 cut(s) 439
KspI CCGCGG 1 cut(s) 957
Kzo9I GATC 5 cut(s) 76, 298, 433, 439, 758
LmnI GCTCC 1 cut(s) 754
Lsp1109I GCAGC 3 cut(s) 172, 544, 571
LweI GCATC 2 cut(s) 94, 569
MaeI CTAG 1 cut(s) 1086
MaeII ACGT 1 cut(s) 1152
MaeIII GTNAC 2 cut(s) 355, 1081
MalI GATC 5 cut(s) 78, 300, 435, 441, 760
MboI GATC 5 cut(s) 76, 298, 433, 439, 758
MboII GAAGA 6 cut(s) 884, 988, 1227, 1235, 1306, 1406
MfeI CAATTG 1 cut(s) 492
MflI RGATCY 1 cut(s) 298
MhlI GDGCHC 1 cut(s) 96
MlsI TGGCCA 1 cut(s) 1419
MluCI AATT 3 cut(s) 426, 492, 1252
MluNI TGGCCA 1 cut(s) 1419
MlyI GAGTC 3 cut(s) 390, 649, 809
MmeI TCCRAC 1 cut(s) 247
Mox20I TGGCCA 1 cut(s) 1419
MroXI GAANNNNTTC 1 cut(s) 832
MscI TGGCCA 1 cut(s) 1419
MseI TTAA 4 cut(s) 44, 222, 650, 1100
MslI CAYNNNNRTG 1 cut(s) 173
Msp20I TGGCCA 1 cut(s) 1419
MspA1I CMGCKG 1 cut(s) 956
MspCI CTTAAG 2 cut(s) 43, 1099
MspR9I CCNGG 4 cut(s) 484, 710, 1219, 1283
MunI CAATTG 1 cut(s) 492
MvaI CCWGG 4 cut(s) 484, 710, 1219, 1283
MvnI CGCG 1 cut(s) 956
MwoI GCNNNNNNNGC 4 cut(s) 590, 797, 1033, 1277
NdeII GATC 5 cut(s) 76, 298, 433, 439, 758
NlaIII CATG 7 cut(s) 163, 542, 733, 804, 995, 1163, 1375
NlaIV GGNNCC 2 cut(s) 960, 1028
NmuCI GTSAC 2 cut(s) 355, 1081
NspI RCATGY 1 cut(s) 163
NspV TTCGAA 1 cut(s) 1349
PdmI GAANNNNTTC 1 cut(s) 832
PfeI GAWTC 2 cut(s) 275, 1204
PkrI GCNGC 4 cut(s) 187, 559, 586, 1298
PleI GAGTC 3 cut(s) 389, 649, 809
PpsI GAGTC 3 cut(s) 389, 649, 809
PpuMI RGGWCCY 1 cut(s) 226
PsiI TTATAA 1 cut(s) 339
Psp124BI GAGCTC 1 cut(s) 96
Psp5II RGGWCCY 1 cut(s) 226
Psp6I CCWGG 4 cut(s) 482, 708, 1217, 1281
PspGI CCWGG 4 cut(s) 482, 708, 1217, 1281
PspN4I GGNNCC 2 cut(s) 960, 1028
PspPI GGNCC 3 cut(s) 226, 775, 1026
PspPPI RGGWCCY 1 cut(s) 226
PsrI GAACNNNNNNTAC 2 cut(s) 1046, 1078
PsuI RGATCY 1 cut(s) 298
RsaI GTAC 6 cut(s) 85, 181, 573, 960, 1064, 1216
RsaNI GTAC 6 cut(s) 84, 180, 572, 959, 1063, 1215
RseI CAYNNNNRTG 1 cut(s) 173
SacI GAGCTC 1 cut(s) 96
SacII CCGCGG 1 cut(s) 957
SaqAI TTAA 4 cut(s) 44, 222, 650, 1100
SatI GCNGC 4 cut(s) 186, 558, 585, 1297
Sau3AI GATC 5 cut(s) 76, 298, 433, 439, 758
Sau96I GGNCC 3 cut(s) 226, 775, 1026
ScaI AGTACT 2 cut(s) 181, 573
SchI GAGTC 3 cut(s) 390, 649, 809
ScrFI CCNGG 4 cut(s) 484, 710, 1219, 1283
SduI GDGCHC 1 cut(s) 96
SfaNI GCATC 2 cut(s) 94, 569
SfcI CTRYAG 2 cut(s) 680, 849
Sfr303I CCGCGG 1 cut(s) 957
SfuI TTCGAA 1 cut(s) 1349
SgrBI CCGCGG 1 cut(s) 957
SinI GGWCC 2 cut(s) 226, 775
SmiMI CAYNNNNRTG 1 cut(s) 173
SmlI CTYRAG 2 cut(s) 43, 1099
SmoI CTYRAG 2 cut(s) 43, 1099
Sse9I AATT 3 cut(s) 426, 492, 1252
SsiI CCGC 4 cut(s) 954, 956, 1294, 1297
SspMI CTAG 1 cut(s) 1086
SstI GAGCTC 1 cut(s) 96
StyD4I CCNGG 4 cut(s) 482, 708, 1217, 1281
TaaI ACNGT 3 cut(s) 22, 234, 1264
TaiI ACGT 1 cut(s) 1155
TaqI TCGA 5 cut(s) 96, 769, 781, 895, 1349
TasI AATT 3 cut(s) 426, 492, 1252
TatI WGTACW 3 cut(s) 179, 571, 1062
TauI GCSGC 1 cut(s) 1299
TfiI GAWTC 2 cut(s) 275, 1204
Tru1I TTAA 4 cut(s) 44, 222, 650, 1100
Tru9I TTAA 4 cut(s) 44, 222, 650, 1100
TscAI CASTG 2 cut(s) 27, 1282
TseFI GTSAC 2 cut(s) 355, 1081
TseI GCWGC 3 cut(s) 185, 557, 584
Tsp45I GTSAC 2 cut(s) 355, 1081
TspDTI ATGAA 1 cut(s) 241
TspGWI ACGGA 2 cut(s) 1180, 1307
TspRI CASTG 2 cut(s) 27, 1282
Vha464I CTTAAG 2 cut(s) 43, 1099
VpaK11BI GGWCC 2 cut(s) 226, 775
XapI RAATTY 1 cut(s) 426
XceI RCATGY 1 cut(s) 163
XcmI CCANNNNNNNNNTGG 1 cut(s) 210
XmnI GAANNNNTTC 1 cut(s) 832
XspI CTAG 1 cut(s) 1086
ZrmI AGTACT 2 cut(s) 181, 573
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.