AT2G32580

Protein of unknown function (DUF1068)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0010168
Physical Location & Seq
Forward (+)
13827425 .. 13829317
1893 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G32580.2

Sequence Viewer

Length: 552 bp
ATGGCGAAGCACACGGCAGCGTTGAAGGTGGGACTAGCTTTACTGGCCCTCAGCATGATTGGCTACATACTTGGTCCACCGCTCTACTGGCACCTCACGGAAGCCTTAGCCGTTTCAGCCACTTCTTGCTCTGCTTGCGTCTGTGATTGCTCTTCGTTGCCTCTTCTCACCATCCCAACTGGACTCAGCAATGGTTCATTCACTGATTGTGCAAAGCGTGATCCAGAGGTGAACGAAGACACAGAGAAGAACTACGCTGAACTTTTAACAGAGGAACTGAAGCAGCGTGAAGCAGCATCGATGGAGAAACACAAACGAGTAGACACGGGTCTGCTAGAGGCCAAGAAGATAACATCATCTTACCAAAAGGAGGCAGATAAGTGTAACTCGGGTATGGAGACTTGTGAAGAAGCAAGAGAGAAGGCGGAGAAAGCACTTGTGGAGCAGAAGAAGCTGACTTCTATGTGGGAACAGAGAGCTCGTCAGAAAGGATACAAAGATGGAGCCACCAAGTCAACTGTTAAATCCAAAAGCGGCACTGAGGTTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1068

Protein Analysis

183

Amino Acids

19.97

Weight (kDa)

6.83

Isoelectric Point (pI)

30.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1068 PF06364 3 - 105 2.7e-48 Protein of unknown function (DUF1068)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010168)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05070 AT2G32580 AT2G32580
fragaria_vesca FvH4_6g42750
malus_domestica MD09G1063300.v1.1 MD09G1071500.v1.1 MD17G1100600.v1.1
prunus_persica Prupe.3G213900_v2.0.a1
pyrus_communis pycom09g03460 pycom12g05330 pycom17g09540
rosa_chinensis RchiOBHm_Chr2g0158641
rosa_laevigata RLG00000021102
rosa_multiflora Rmu_sc0039916.1_g000001
rosa_roxburghii Rroxscaffold_2G00091150
rosa_rugosa Rorug02G0471000
rosa_samantha Rh2AG536800 Rh2BG550000 Rh2CG520500 Rh2DG559600
rosa_wichuraiana Rw2G044440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 90
AccBSI CCGCTC 1 cut(s) 82
AccI GTMKAC 1 cut(s) 321
AciI CCGC 3 cut(s) 80, 425, 534
AclWI GGATC 1 cut(s) 215
AcuI CTGAAG 1 cut(s) 299
AfiI CCNNNNNNNGG 1 cut(s) 370
AgsI TTSAA 1 cut(s) 25
AluBI AGCT 3 cut(s) 38, 454, 479
AluI AGCT 3 cut(s) 38, 454, 479
Alw21I GWGCWC 1 cut(s) 481
Alw26I GTCTC 1 cut(s) 392
AlwI GGATC 1 cut(s) 215
Ama87I CYCGRG 1 cut(s) 388
AoxI GGCC 2 cut(s) 45, 339
ApeKI GCWGC 3 cut(s) 17, 283, 293
AspS9I GGNCC 2 cut(s) 46, 74
AsuHPI GGTGA 2 cut(s) 160, 241
AvaI CYCGRG 1 cut(s) 388
AvaII GGWCC 1 cut(s) 74
BanI GGYRCC 1 cut(s) 90
BanII GRGCYC 1 cut(s) 481
BbsI GAAGAC 1 cut(s) 243
Bbv12I GWGCWC 1 cut(s) 481
BbvCI CCTCAGC 1 cut(s) 50
BbvI GCAGC 3 cut(s) 29, 295, 305
BccI CCATC 3 cut(s) 179, 295, 494
BceAI ACGGC 2 cut(s) 30, 95
BciVI GTATCC 1 cut(s) 485
BcoDI GTCTC 1 cut(s) 392
BfaI CTAG 2 cut(s) 35, 335
BfuI GTATCC 1 cut(s) 485
BisI GCNGC 4 cut(s) 18, 284, 294, 535
BlsI GCNGC 4 cut(s) 19, 285, 295, 536
Bme18I GGWCC 1 cut(s) 74
BmeT110I CYCGRG 1 cut(s) 388
BmgT120I GGNCC 2 cut(s) 46, 74
BmiI GGNNCC 2 cut(s) 92, 505
BmsI GCATC 1 cut(s) 305
BoxI GACNNNNGTC 1 cut(s) 327
BpiI GAAGAC 1 cut(s) 243
Bpu10I CCTNAGC 2 cut(s) 50, 106
Bsa29I ATCGAT 1 cut(s) 299
Bsc4I CCNNNNNNNGG 1 cut(s) 370
Bse1I ACTGG 3 cut(s) 48, 92, 184
Bse3DI GCAATG 1 cut(s) 196
BseCI ATCGAT 1 cut(s) 299
BseGI GGATG 1 cut(s) 171
BseLI CCNNNNNNNGG 1 cut(s) 370
BseMI GCAATG 1 cut(s) 196
BseMII CTCAG 3 cut(s) 64, 199, 531
BseNI ACTGG 3 cut(s) 48, 92, 184
BseXI GCAGC 3 cut(s) 29, 295, 305
BshFI GGCC 2 cut(s) 47, 341
BshNI GGYRCC 1 cut(s) 90
BshVI ATCGAT 1 cut(s) 299
BsiHKAI GWGCWC 1 cut(s) 481
BsiHKCI CYCGRG 1 cut(s) 388
BslFI GGGAC 1 cut(s) 45
BslI CCNNNNNNNGG 1 cut(s) 370
BsmAI GTCTC 1 cut(s) 392
BsmFI GGGAC 1 cut(s) 45
BsnI GGCC 2 cut(s) 47, 341
BsoBI CYCGRG 1 cut(s) 388
Bsp1286I GDGCHC 1 cut(s) 481
Bsp143I GATC 1 cut(s) 220
BspACI CCGC 3 cut(s) 80, 425, 534
BspANI GGCC 2 cut(s) 47, 341
BspCNI CTCAG 3 cut(s) 63, 198, 532
BspDI ATCGAT 1 cut(s) 299
BspLI GGNNCC 2 cut(s) 92, 505
BspPI GGATC 1 cut(s) 215
BspQI GCTCTTC 1 cut(s) 157
BspT107I GGYRCC 1 cut(s) 90
BsrBI CCGCTC 1 cut(s) 82
BsrDI GCAATG 1 cut(s) 196
BsrI ACTGG 3 cut(s) 48, 92, 184
BssMI GATC 1 cut(s) 220
Bst4CI ACNGT 1 cut(s) 520
Bst6I CTCTTC 2 cut(s) 157, 168
BstC8I GCNNGC 1 cut(s) 136
BstDEI CTNAG 4 cut(s) 50, 106, 185, 540
BstF5I GGATG 1 cut(s) 171
BstKTI GATC 1 cut(s) 223
BstMAI GTCTC 1 cut(s) 392
BstMBI GATC 1 cut(s) 220
BstMWI GCNNNNNNNGC 7 cut(s) 44, 60, 88, 116, 135, 431, 451
BstPAI GACNNNNGTC 1 cut(s) 327
BstV1I GCAGC 3 cut(s) 29, 295, 305
BstV2I GAAGAC 1 cut(s) 243
Bsu15I ATCGAT 1 cut(s) 299
BsuI GTATCC 1 cut(s) 485
BsuRI GGCC 2 cut(s) 47, 341
BsuTUI ATCGAT 1 cut(s) 299
BtsCI GGATG 1 cut(s) 171
BtsIMutI CAGTG 2 cut(s) 201, 537
Cac8I GCNNGC 1 cut(s) 136
Cfr13I GGNCC 2 cut(s) 46, 74
ClaI ATCGAT 1 cut(s) 299
CseI GACGC 1 cut(s) 127
CviAII CATG 1 cut(s) 55
DdeI CTNAG 4 cut(s) 50, 106, 185, 540
DpnI GATC 1 cut(s) 222
DpnII GATC 1 cut(s) 220
Eam1104I CTCTTC 2 cut(s) 157, 168
EarI CTCTTC 2 cut(s) 157, 168
EciI GGCGGA 1 cut(s) 440
Ecl136II GAGCTC 1 cut(s) 479
Eco24I GRGCYC 1 cut(s) 481
Eco47I GGWCC 1 cut(s) 74
Eco53kI GAGCTC 1 cut(s) 479
Eco57I CTGAAG 1 cut(s) 299
Eco88I CYCGRG 1 cut(s) 388
EcoICRI GAGCTC 1 cut(s) 479
EcoT38I GRGCYC 1 cut(s) 481
FaeI CATG 1 cut(s) 58
FaiI YATR 4 cut(s) 56, 68, 395, 464
FaqI GGGAC 1 cut(s) 45
FatI CATG 1 cut(s) 54
FblI GTMKAC 1 cut(s) 321
Fnu4HI GCNGC 4 cut(s) 18, 284, 294, 535
FokI GGATG 1 cut(s) 158
FriOI GRGCYC 1 cut(s) 481
Fsp4HI GCNGC 4 cut(s) 18, 284, 294, 535
FspBI CTAG 2 cut(s) 35, 335
GluI GCNGC 4 cut(s) 18, 284, 294, 535
HaeIII GGCC 2 cut(s) 47, 341
HgaI GACGC 1 cut(s) 127
Hin1II CATG 1 cut(s) 58
HincII GTYRAC 1 cut(s) 516
HindII GTYRAC 1 cut(s) 516
HinfI GANTC 1 cut(s) 183
HphI GGTGA 2 cut(s) 160, 241
Hpy166II GTNNAC 4 cut(s) 77, 232, 322, 516
Hpy188I TCNGA 1 cut(s) 486
Hpy188III TCNNGA 1 cut(s) 224
Hpy8I GTNNAC 4 cut(s) 77, 232, 322, 516
HpyAV CCTTC 2 cut(s) 19, 415
HpyCH4III ACNGT 1 cut(s) 520
HpyCH4V TGCA 1 cut(s) 212
HpyF10VI GCNNNNNNNGC 7 cut(s) 44, 60, 88, 116, 135, 431, 451
HpyF3I CTNAG 4 cut(s) 50, 106, 185, 540
Hsp92II CATG 1 cut(s) 58
Kzo9I GATC 1 cut(s) 220
LguI GCTCTTC 1 cut(s) 157
LmnI GCTCC 2 cut(s) 442, 503
LpnPI CCDG 4 cut(s) 29, 73, 165, 237
Lsp1109I GCAGC 3 cut(s) 29, 295, 305
LweI GCATC 1 cut(s) 305
MaeI CTAG 2 cut(s) 35, 335
MaeIII GTNAC 1 cut(s) 383
MalI GATC 1 cut(s) 222
MbiI CCGCTC 1 cut(s) 82
MboI GATC 1 cut(s) 220
MboII GAAGA 7 cut(s) 144, 155, 248, 259, 358, 419, 460
MhlI GDGCHC 1 cut(s) 481
MlyI GAGTC 1 cut(s) 177
MnlI CCTC 8 cut(s) 59, 104, 171, 220, 265, 331, 364, 535
MseI TTAA 3 cut(s) 266, 522, 550
MwoI GCNNNNNNNGC 7 cut(s) 44, 60, 88, 116, 135, 431, 451
NdeII GATC 1 cut(s) 220
NlaIII CATG 1 cut(s) 58
NlaIV GGNNCC 2 cut(s) 92, 505
PciSI GCTCTTC 1 cut(s) 157
PkrI GCNGC 4 cut(s) 19, 285, 295, 536
PleI GAGTC 1 cut(s) 177
PpsI GAGTC 1 cut(s) 177
PshAI GACNNNNGTC 1 cut(s) 327
Psp124BI GAGCTC 1 cut(s) 481
PspN4I GGNNCC 2 cut(s) 92, 505
PspPI GGNCC 2 cut(s) 46, 74
SacI GAGCTC 1 cut(s) 481
SapI GCTCTTC 1 cut(s) 157
SaqAI TTAA 3 cut(s) 266, 522, 550
SatI GCNGC 4 cut(s) 18, 284, 294, 535
Sau3AI GATC 1 cut(s) 220
Sau96I GGNCC 2 cut(s) 46, 74
SchI GAGTC 1 cut(s) 177
SduI GDGCHC 1 cut(s) 481
SetI ASST 7 cut(s) 30, 40, 96, 231, 456, 481, 546
SfaNI GCATC 1 cut(s) 305
SinI GGWCC 1 cut(s) 74
SsiI CCGC 3 cut(s) 80, 425, 534
SspMI CTAG 2 cut(s) 35, 335
SstI GAGCTC 1 cut(s) 481
TaaI ACNGT 1 cut(s) 520
TaqI TCGA 1 cut(s) 299
TauI GCSGC 1 cut(s) 537
Tru1I TTAA 3 cut(s) 266, 522, 550
Tru9I TTAA 3 cut(s) 266, 522, 550
TscAI CASTG 2 cut(s) 208, 544
TseI GCWGC 3 cut(s) 17, 283, 293
TspDTI ATGAA 1 cut(s) 186
TspGWI ACGGA 1 cut(s) 113
TspRI CASTG 2 cut(s) 208, 544
VpaK11BI GGWCC 1 cut(s) 74
XcmI CCANNNNNNNNNTGG 1 cut(s) 84
XmiI GTMKAC 1 cut(s) 321
XspI CTAG 2 cut(s) 35, 335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.