pycom12g05330

maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0010168
Physical Location & Seq
Forward (+)
5188857 .. 5189568
712 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g05330.1

Sequence Viewer

Length: 453 bp
ATGCTCCAAAAACCTAGCCAAAACCCTTGTTTTGCAGGTTATCCAATGCTCCAAAACCTAGCCAGCCAAAGGCAAATTGGTTTGCAGTACTGTTACCCTTCATTTGCTAACAATTCTCAACTTTTTTTTGTGTATTGTTTAGTAGAATCAGGAATTTACCCTTTTGTACGCGTGTCCGCCATCGTAGAAAGGAAGGGGATGGGTGGCACTCTTCTTCTGCTTCACTCGGGTGGATCTCTGGAATTCTCCTTAGGAGTTGAAGCTTTGGAAAATCAGCAACGAGCTCATATGGCTCTGCTGGAGGCTAAGAAGATTGCATCCCAGTATCAAAAGGAAGCAGACAAGTGCAATTCAGGGATGGAAACATGTGAAGAAGCAAGGGAGAAAGCTGAAGCAACAGTAGAGGCACAGCGGAAACAGACTGCACGTGCTGCCTCTTCCGTTTTGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DRIM DUF1068

Protein Analysis

151

Amino Acids

16.56

Weight (kDa)

7.61

Isoelectric Point (pI)

31.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1068 PF06364 86 - 142 1.8e-23 Protein of unknown function (DUF1068)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010168)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05070 AT2G32580 AT2G32580
fragaria_vesca FvH4_6g42750
malus_domestica MD09G1063300.v1.1 MD09G1071500.v1.1 MD17G1100600.v1.1
prunus_persica Prupe.3G213900_v2.0.a1
pyrus_communis pycom09g03460 pycom12g05330 pycom17g09540
rosa_chinensis RchiOBHm_Chr2g0158641
rosa_laevigata RLG00000021102
rosa_multiflora Rmu_sc0039916.1_g000001
rosa_roxburghii Rroxscaffold_2G00091150
rosa_rugosa Rorug02G0471000
rosa_samantha Rh2AG536800 Rh2BG550000 Rh2CG520500 Rh2DG559600
rosa_wichuraiana Rw2G044440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 26
AccII CGCG 1 cut(s) 171
AciI CCGC 2 cut(s) 177, 412
AclWI GGATC 1 cut(s) 241
AcsI RAATTY 2 cut(s) 153, 242
AcuI CTGAAG 1 cut(s) 411
AcvI CACGTG 1 cut(s) 428
AfaI GTAC 2 cut(s) 89, 168
AfiI CCNNNNNNNGG 1 cut(s) 69
AflIII ACRYGT 2 cut(s) 169, 365
AgsI TTSAA 1 cut(s) 260
AleI CACNNNNGTG 1 cut(s) 228
AluBI AGCT 3 cut(s) 263, 284, 389
AluI AGCT 3 cut(s) 263, 284, 389
Alw21I GWGCWC 1 cut(s) 286
AlwI GGATC 1 cut(s) 241
Ama87I CYCGRG 1 cut(s) 226
ApeKI GCWGC 1 cut(s) 431
ApoI RAATTY 2 cut(s) 153, 242
AvaI CYCGRG 1 cut(s) 226
AxyI CCTNAGG 1 cut(s) 250
BanII GRGCYC 1 cut(s) 286
BbrPI CACGTG 1 cut(s) 428
Bbv12I GWGCWC 1 cut(s) 286
BbvI GCAGC 1 cut(s) 418
BccI CCATC 3 cut(s) 188, 193, 352
BfaI CTAG 2 cut(s) 15, 59
BfuAI ACCTGC 1 cut(s) 26
BisI GCNGC 1 cut(s) 432
BlsI GCNGC 1 cut(s) 433
BmcAI AGTACT 1 cut(s) 89
BmeT110I CYCGRG 1 cut(s) 226
BmrI ACTGGG 1 cut(s) 316
BmsI GCATC 1 cut(s) 326
BmuI ACTGGG 1 cut(s) 316
BpmI CTGGAG 1 cut(s) 320
BsaAI YACGTR 1 cut(s) 428
Bsc4I CCNNNNNNNGG 1 cut(s) 69
Bse1I ACTGG 1 cut(s) 322
Bse21I CCTNAGG 1 cut(s) 250
BseGI GGATG 3 cut(s) 204, 317, 363
BseLI CCNNNNNNNGG 1 cut(s) 69
BseNI ACTGG 1 cut(s) 322
BseXI GCAGC 1 cut(s) 418
BsgI GTGCAG 1 cut(s) 408
Bsh1236I CGCG 1 cut(s) 171
BsiHKAI GWGCWC 1 cut(s) 286
BsiHKCI CYCGRG 1 cut(s) 226
BslI CCNNNNNNNGG 1 cut(s) 69
BsoBI CYCGRG 1 cut(s) 226
Bsp1286I GDGCHC 1 cut(s) 286
Bsp143I GATC 1 cut(s) 233
BspACI CCGC 2 cut(s) 177, 412
BspFNI CGCG 1 cut(s) 171
BspMI ACCTGC 1 cut(s) 26
BspPI GGATC 1 cut(s) 241
BsrI ACTGG 1 cut(s) 322
BssMI GATC 1 cut(s) 233
Bst4CI ACNGT 2 cut(s) 92, 400
Bst6I CTCTTC 2 cut(s) 216, 442
BstAPI GCANNNNNTGC 1 cut(s) 431
BstBAI YACGTR 1 cut(s) 428
BstC8I GCNNGC 1 cut(s) 64
BstDEI CTNAG 2 cut(s) 250, 306
BstF5I GGATG 3 cut(s) 204, 317, 363
BstFNI CGCG 1 cut(s) 171
BstKTI GATC 1 cut(s) 236
BstMBI GATC 1 cut(s) 233
BstMWI GCNNNNNNNGC 2 cut(s) 290, 431
BstNSI RCATGY 1 cut(s) 369
BstUI CGCG 1 cut(s) 171
BstV1I GCAGC 1 cut(s) 418
BstX2I RGATCY 1 cut(s) 233
BstYI RGATCY 1 cut(s) 233
Bsu36I CCTNAGG 1 cut(s) 250
BtsCI GGATG 3 cut(s) 204, 317, 363
BveI ACCTGC 1 cut(s) 26
Cac8I GCNNGC 1 cut(s) 64
Csp6I GTAC 2 cut(s) 88, 167
CviAII CATG 1 cut(s) 366
CviJI RGCY 8 cut(s) 18, 62, 66, 263, 284, 293, 305, 389
CviKI_1 RGCY 8 cut(s) 18, 62, 66, 263, 284, 293, 305, 389
CviQI GTAC 2 cut(s) 88, 167
DdeI CTNAG 2 cut(s) 250, 306
DpnI GATC 1 cut(s) 235
DpnII GATC 1 cut(s) 233
Eam1104I CTCTTC 2 cut(s) 216, 442
EarI CTCTTC 2 cut(s) 216, 442
EciI GGCGGA 1 cut(s) 166
Ecl136II GAGCTC 1 cut(s) 284
Eco24I GRGCYC 1 cut(s) 286
Eco53kI GAGCTC 1 cut(s) 284
Eco57I CTGAAG 1 cut(s) 411
Eco72I CACGTG 1 cut(s) 428
Eco81I CCTNAGG 1 cut(s) 250
Eco88I CYCGRG 1 cut(s) 226
EcoICRI GAGCTC 1 cut(s) 284
EcoRI GAATTC 1 cut(s) 242
EcoT38I GRGCYC 1 cut(s) 286
FaeI CATG 1 cut(s) 369
FaiI YATR 3 cut(s) 288, 290, 367
FatI CATG 1 cut(s) 365
FauNDI CATATG 1 cut(s) 288
Fnu4HI GCNGC 1 cut(s) 432
FokI GGATG 3 cut(s) 211, 304, 370
FriOI GRGCYC 1 cut(s) 286
Fsp4HI GCNGC 1 cut(s) 432
FspBI CTAG 2 cut(s) 15, 59
GluI GCNGC 1 cut(s) 432
GsuI CTGGAG 1 cut(s) 320
Hin1II CATG 1 cut(s) 369
HindIII AAGCTT 1 cut(s) 261
HinfI GANTC 1 cut(s) 146
Hpy188III TCNNGA 2 cut(s) 150, 239
HpyAV CCTTC 2 cut(s) 108, 187
HpyCH4III ACNGT 2 cut(s) 92, 400
HpyCH4IV ACGT 1 cut(s) 427
HpyCH4V TGCA 5 cut(s) 35, 85, 317, 348, 425
HpyF10VI GCNNNNNNNGC 2 cut(s) 290, 431
HpyF3I CTNAG 2 cut(s) 250, 306
HpySE526I ACGT 1 cut(s) 427
Hsp92II CATG 1 cut(s) 369
Kzo9I GATC 1 cut(s) 233
LmnI GCTCC 2 cut(s) 9, 54
LpnPI CCDG 7 cut(s) 21, 76, 135, 224, 284, 335, 339
Lsp1109I GCAGC 1 cut(s) 418
LweI GCATC 1 cut(s) 326
MaeI CTAG 2 cut(s) 15, 59
MaeII ACGT 1 cut(s) 427
MaeIII GTNAC 1 cut(s) 92
MalI GATC 1 cut(s) 235
MboI GATC 1 cut(s) 233
MboII GAAGA 5 cut(s) 203, 206, 322, 383, 429
MflI RGATCY 1 cut(s) 233
MhlI GDGCHC 1 cut(s) 286
MluCI AATT 5 cut(s) 75, 112, 153, 242, 349
MluI ACGCGT 1 cut(s) 169
MnlI CCTC 3 cut(s) 295, 397, 445
MslI CAYNNNNRTG 1 cut(s) 228
MspA1I CMGCKG 1 cut(s) 412
MvnI CGCG 1 cut(s) 171
MwoI GCNNNNNNNGC 2 cut(s) 290, 431
NdeI CATATG 1 cut(s) 288
NdeII GATC 1 cut(s) 233
NlaIII CATG 1 cut(s) 369
NspI RCATGY 1 cut(s) 369
OliI CACNNNNGTG 1 cut(s) 228
PciI ACATGT 1 cut(s) 365
PfeI GAWTC 1 cut(s) 146
PkrI GCNGC 1 cut(s) 433
PmaCI CACGTG 1 cut(s) 428
PmlI CACGTG 1 cut(s) 428
Ppu21I YACGTR 1 cut(s) 428
PscI ACATGT 1 cut(s) 365
Psp124BI GAGCTC 1 cut(s) 286
PspCI CACGTG 1 cut(s) 428
PsuI RGATCY 1 cut(s) 233
RsaI GTAC 2 cut(s) 89, 168
RsaNI GTAC 2 cut(s) 88, 167
RseI CAYNNNNRTG 1 cut(s) 228
SacI GAGCTC 1 cut(s) 286
SatI GCNGC 1 cut(s) 432
Sau3AI GATC 1 cut(s) 233
ScaI AGTACT 1 cut(s) 89
SduI GDGCHC 1 cut(s) 286
SetI ASST 7 cut(s) 16, 40, 60, 265, 286, 391, 430
SfaNI GCATC 1 cut(s) 326
SmiMI CAYNNNNRTG 1 cut(s) 228
Sse9I AATT 5 cut(s) 75, 112, 153, 242, 349
SsiI CCGC 2 cut(s) 177, 412
SspMI CTAG 2 cut(s) 15, 59
SstI GAGCTC 1 cut(s) 286
TaaI ACNGT 2 cut(s) 92, 400
TaiI ACGT 1 cut(s) 430
TasI AATT 5 cut(s) 75, 112, 153, 242, 349
TatI WGTACW 1 cut(s) 87
TfiI GAWTC 1 cut(s) 146
TseI GCWGC 1 cut(s) 431
TspDTI ATGAA 1 cut(s) 90
TspGWI ACGGA 1 cut(s) 430
XapI RAATTY 2 cut(s) 153, 242
XceI RCATGY 1 cut(s) 369
XcmI CCANNNNNNNNNTGG 1 cut(s) 74
XspI CTAG 2 cut(s) 15, 59
ZrmI AGTACT 1 cut(s) 89
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.