AT2G33250

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0010950
Physical Location & Seq
Forward (+)
14097369 .. 14098751
1383 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G33250.1

Sequence Viewer

Length: 900 bp
ATGGCGGATAGAGGAGCTCTTTCGTTACCGCGAGCTTCTACTTATCTTAGTTTCTCAAACCTCCATGTCTGGCGATTCCACAACCCTAGATCTTATCCTCCCTCTCGTTCTCAACTCCTCTGTTTCTCTTCCTCATGCTCTAGAGTAACTTCGGCGAAGATAATTCAGCTGAACCGCCGGAGATTCAGCTATAAAATATGCGCCGCCGCTGATGACGGTAGGGGATACCGTTTGACGGAAAGGGGAAAAAGGGGAAGGAAGCGGCGGGAGCTATGGGAAGAACTTTTTGAAGATAATGTTGAAGACGATGATGATGAAGATGATGGTGGTGGTGGAATTGGTAGTGCTAATTTTGACCTGTGGAAGATCTTAGAGGAAATTGTAGATAATGTTTGGATTTTAAAGGCATTCAAATCGTATGGATATCTCCTCCCATTCATCATCTTATCGCTTTTCTTCAGCACCGGACCCAAAGCTTTCCTCGTGTCACTAGCCGTAGCAATCGGGCCATCATTGTTATTTTATGCGTTCCAGAAGTTGATTGGTTGGGATAAACGCAGAGGTACATCAATAGCAAATCAATTCGGGATAGAGGAAGAAGAAGAAGAAGTGGAGAGAAGTAGTAGCAGGATTCGATACAACCCATCAACCGTTAGAAACAATGTCAATGGCCGTGGAGTTAACCGTAGTTCAGCTGGAATGGCTTCTAAGTTTGGTGGGTGGGACGAGTTAGACGGGCTTGGTACCACCATTCCCGAACGGCCAACAAGCGAACCGAAGAAGAAACCGTTACCGAAGAGGAAGAGAGTAAGAAGAGAAAAGGCAGCAGAACCATTATTGTTGAGATTGTTGGTGTCTTTGTTTCCATTCTTAAGCACATACACAAACATGCTCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

299

Amino Acids

34.0

Weight (kDa)

9.66

Isoelectric Point (pI)

57.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010950)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G33250 AT3G04310 AT3G04310
fragaria_vesca FvH4_4g05190 FvH4_4g05190
malus_domestica MD13G1225800.v1.1 MD16G1230800.v1.1
prunus_persica Prupe.1G052600_v2.0.a1
pyrus_communis pycom16g19270
rosa_chinensis RchiOBHm_Chr4g0396101
rosa_laevigata RLG00000009572
rosa_multiflora Rmu_sc0006325.1_g000019 Rmu_sc0007010.1_g000013 Rmu_sc0009634.1_g000010
rosa_roxburghii Rroxscaffold_5G00340900
rosa_rugosa Rorug03G0352600
rosa_samantha Rh4BG064200 Rh4CG070800 Rh4DG061200
rosa_wichuraiana Rw4G005330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 743
AccB1I GGYRCC 1 cut(s) 743
AccII CGCG 1 cut(s) 31
AciI CCGC 7 cut(s) 5, 29, 175, 204, 207, 262, 265
AcoI YGGCCR 2 cut(s) 670, 761
AcuI CTGAAG 1 cut(s) 442
AfaI GTAC 2 cut(s) 565, 745
AfiI CCNNNNNNNGG 1 cut(s) 235
AflII CTTAAG 1 cut(s) 871
AgsI TTSAA 3 cut(s) 290, 302, 412
AluBI AGCT 7 cut(s) 17, 35, 169, 189, 271, 476, 695
AluI AGCT 7 cut(s) 17, 35, 169, 189, 271, 476, 695
Alw21I GWGCWC 1 cut(s) 19
AoxI GGCC 3 cut(s) 506, 670, 761
ApeKI GCWGC 1 cut(s) 824
Asp700I GAANNNNTTC 1 cut(s) 703
Asp718I GGTACC 1 cut(s) 743
AspLEI GCGC 1 cut(s) 203
AspS9I GGNCC 2 cut(s) 467, 506
AvaII GGWCC 1 cut(s) 467
BanI GGYRCC 1 cut(s) 743
BanII GRGCYC 1 cut(s) 19
BauI CACGAG 1 cut(s) 482
BbsI GAAGAC 1 cut(s) 309
Bbv12I GWGCWC 1 cut(s) 19
BbvI GCAGC 1 cut(s) 836
BccI CCATC 3 cut(s) 317, 517, 652
BceAI ACGGC 3 cut(s) 479, 657, 776
BcgI CGANNNNNNTGC 2 cut(s) 396, 430
BciVI GTATCC 1 cut(s) 218
BfaI CTAG 3 cut(s) 87, 141, 491
BfrI CTTAAG 1 cut(s) 871
BfuI GTATCC 1 cut(s) 218
BglII AGATCT 2 cut(s) 89, 366
BisI GCNGC 4 cut(s) 204, 207, 263, 825
BlsI GCNGC 4 cut(s) 205, 208, 264, 826
Bme18I GGWCC 1 cut(s) 467
BmgT120I GGNCC 2 cut(s) 467, 506
BmiI GGNNCC 2 cut(s) 469, 745
BpiI GAAGAC 1 cut(s) 309
BpuEI CTTGAG 1 cut(s) 878
BsaJI CCNNGG 1 cut(s) 673
BsaWI WCCGGW 1 cut(s) 464
Bsc4I CCNNNNNNNGG 1 cut(s) 235
BseDI CCNNGG 1 cut(s) 673
BseLI CCNNNNNNNGG 1 cut(s) 235
BseRI GAGGAG 3 cut(s) 27, 107, 419
BseXI GCAGC 1 cut(s) 836
Bsh1236I CGCG 1 cut(s) 31
BshFI GGCC 3 cut(s) 508, 672, 763
BshNI GGYRCC 1 cut(s) 743
BsiHKAI GWGCWC 1 cut(s) 19
BsiSI CCGG 2 cut(s) 178, 465
BslFI GGGAC 1 cut(s) 737
BslI CCNNNNNNNGG 1 cut(s) 235
BsmFI GGGAC 1 cut(s) 737
BsmI GAATGC 1 cut(s) 407
BsnI GGCC 3 cut(s) 508, 672, 763
Bsp1286I GDGCHC 1 cut(s) 19
Bsp143I GATC 2 cut(s) 89, 366
BspACI CCGC 7 cut(s) 5, 29, 175, 204, 207, 262, 265
BspANI GGCC 3 cut(s) 508, 672, 763
BspFNI CGCG 1 cut(s) 31
BspLI GGNNCC 2 cut(s) 469, 745
BspT107I GGYRCC 1 cut(s) 743
BspTI CTTAAG 1 cut(s) 871
BssECI CCNNGG 1 cut(s) 673
BssMI GATC 2 cut(s) 89, 366
BssSI CACGAG 1 cut(s) 482
Bst2BI CACGAG 1 cut(s) 482
Bst4CI ACNGT 5 cut(s) 218, 230, 652, 686, 789
Bst6I CTCTTC 4 cut(s) 133, 791, 797, 808
BstAFI CTTAAG 1 cut(s) 871
BstC8I GCNNGC 1 cut(s) 33
BstDEI CTNAG 3 cut(s) 47, 370, 708
BstDSI CCRYGG 1 cut(s) 673
BstFNI CGCG 1 cut(s) 31
BstHHI GCGC 1 cut(s) 203
BstKTI GATC 2 cut(s) 92, 369
BstMBI GATC 2 cut(s) 89, 366
BstMWI GCNNNNNNNGC 2 cut(s) 268, 701
BstNSI RCATGY 1 cut(s) 892
BstUI CGCG 1 cut(s) 31
BstV1I GCAGC 1 cut(s) 836
BstV2I GAAGAC 1 cut(s) 309
BstX2I RGATCY 2 cut(s) 89, 366
BstYI RGATCY 2 cut(s) 89, 366
BsuI GTATCC 1 cut(s) 218
BsuRI GGCC 3 cut(s) 508, 672, 763
BtgI CCRYGG 1 cut(s) 673
Cac8I GCNNGC 1 cut(s) 33
CfoI GCGC 1 cut(s) 203
Cfr13I GGNCC 2 cut(s) 467, 506
Csp6I GTAC 2 cut(s) 564, 744
CspCI CAANNNNNGTGG 2 cut(s) 655, 690
CviAII CATG 3 cut(s) 65, 135, 889
CviQI GTAC 2 cut(s) 564, 744
DdeI CTNAG 3 cut(s) 47, 370, 708
DpnI GATC 2 cut(s) 91, 368
DpnII GATC 2 cut(s) 89, 366
DraI TTTAAA 1 cut(s) 402
EaeI YGGCCR 2 cut(s) 670, 761
Eam1104I CTCTTC 4 cut(s) 133, 791, 797, 808
EarI CTCTTC 4 cut(s) 133, 791, 797, 808
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 17
Eco24I GRGCYC 1 cut(s) 19
Eco32I GATATC 1 cut(s) 425
Eco47I GGWCC 1 cut(s) 467
Eco53kI GAGCTC 1 cut(s) 17
Eco57I CTGAAG 1 cut(s) 442
EcoICRI GAGCTC 1 cut(s) 17
EcoRV GATATC 1 cut(s) 425
EcoT38I GRGCYC 1 cut(s) 19
FaeI CATG 3 cut(s) 68, 138, 892
FaiI YATR 9 cut(s) 66, 136, 192, 199, 274, 420, 525, 880, 890
FaqI GGGAC 1 cut(s) 737
FatI CATG 3 cut(s) 64, 134, 888
FauI CCCGC 1 cut(s) 258
Fnu4HI GCNGC 4 cut(s) 204, 207, 263, 825
FriOI GRGCYC 1 cut(s) 19
Fsp4HI GCNGC 4 cut(s) 204, 207, 263, 825
FspBI CTAG 3 cut(s) 87, 141, 491
GlaI GCGC 1 cut(s) 202
GluI GCNGC 4 cut(s) 204, 207, 263, 825
HaeIII GGCC 3 cut(s) 508, 672, 763
HapII CCGG 2 cut(s) 178, 465
HhaI GCGC 1 cut(s) 203
Hin1II CATG 3 cut(s) 68, 138, 892
Hin6I GCGC 1 cut(s) 201
HinP1I GCGC 1 cut(s) 201
HincII GTYRAC 1 cut(s) 682
HindII GTYRAC 1 cut(s) 682
HindIII AAGCTT 1 cut(s) 474
HinfI GANTC 3 cut(s) 75, 183, 631
HpaI GTTAAC 1 cut(s) 682
HpaII CCGG 2 cut(s) 178, 465
Hpy166II GTNNAC 1 cut(s) 682
Hpy188III TCNNGA 4 cut(s) 141, 532, 586, 755
Hpy8I GTNNAC 1 cut(s) 682
HpyAV CCTTC 1 cut(s) 249
HpyCH4III ACNGT 5 cut(s) 218, 230, 652, 686, 789
HpyF10VI GCNNNNNNNGC 2 cut(s) 268, 701
HpyF3I CTNAG 3 cut(s) 47, 370, 708
Hsp92II CATG 3 cut(s) 68, 138, 892
HspAI GCGC 1 cut(s) 201
KpnI GGTACC 1 cut(s) 747
KspAI GTTAAC 1 cut(s) 682
Kzo9I GATC 2 cut(s) 89, 366
LmnI GCTCC 2 cut(s) 14, 268
LpnPI CCDG 7 cut(s) 55, 191, 371, 478, 545, 613, 681
Lsp1109I GCAGC 1 cut(s) 836
MaeI CTAG 3 cut(s) 87, 141, 491
MaeIII GTNAC 4 cut(s) 24, 145, 486, 789
MalI GATC 2 cut(s) 91, 368
MboI GATC 2 cut(s) 89, 366
MflI RGATCY 2 cut(s) 89, 366
MhlI GDGCHC 1 cut(s) 19
MluCI AATT 5 cut(s) 162, 336, 349, 378, 581
MroXI GAANNNNTTC 1 cut(s) 703
MseI TTAA 3 cut(s) 401, 681, 872
MslI CAYNNNNRTG 1 cut(s) 887
MspA1I CMGCKG 3 cut(s) 169, 209, 695
MspCI CTTAAG 1 cut(s) 871
MspI CCGG 2 cut(s) 178, 465
Mva1269I GAATGC 1 cut(s) 407
MvnI CGCG 1 cut(s) 31
MwoI GCNNNNNNNGC 2 cut(s) 268, 701
NdeII GATC 2 cut(s) 89, 366
NlaIII CATG 3 cut(s) 68, 138, 892
NlaIV GGNNCC 2 cut(s) 469, 745
NmuCI GTSAC 1 cut(s) 486
NspI RCATGY 1 cut(s) 892
PctI GAATGC 1 cut(s) 407
PdmI GAANNNNTTC 1 cut(s) 703
PfeI GAWTC 3 cut(s) 75, 183, 631
PkrI GCNGC 4 cut(s) 205, 208, 264, 826
Psp124BI GAGCTC 1 cut(s) 19
PspN4I GGNNCC 2 cut(s) 469, 745
PspPI GGNCC 2 cut(s) 467, 506
PsuI RGATCY 2 cut(s) 89, 366
PvuII CAGCTG 2 cut(s) 169, 695
RsaI GTAC 2 cut(s) 565, 745
RsaNI GTAC 2 cut(s) 564, 744
RseI CAYNNNNRTG 1 cut(s) 887
SacI GAGCTC 1 cut(s) 19
SaqAI TTAA 3 cut(s) 401, 681, 872
SatI GCNGC 4 cut(s) 204, 207, 263, 825
Sau3AI GATC 2 cut(s) 89, 366
Sau96I GGNCC 2 cut(s) 467, 506
SduI GDGCHC 1 cut(s) 19
SinI GGWCC 1 cut(s) 467
SmiMI CAYNNNNRTG 1 cut(s) 887
SmlI CTYRAG 2 cut(s) 871, 893
SmoI CTYRAG 2 cut(s) 871, 893
Sse9I AATT 5 cut(s) 162, 336, 349, 378, 581
SsiI CCGC 7 cut(s) 5, 29, 175, 204, 207, 262, 265
SspMI CTAG 3 cut(s) 87, 141, 491
SstI GAGCTC 1 cut(s) 19
TaaI ACNGT 5 cut(s) 218, 230, 652, 686, 789
TaqI TCGA 1 cut(s) 634
TasI AATT 5 cut(s) 162, 336, 349, 378, 581
TauI GCSGC 3 cut(s) 206, 209, 265
TfiI GAWTC 3 cut(s) 75, 183, 631
Tru1I TTAA 3 cut(s) 401, 681, 872
Tru9I TTAA 3 cut(s) 401, 681, 872
TseFI GTSAC 1 cut(s) 486
TseI GCWGC 1 cut(s) 824
Tsp45I GTSAC 1 cut(s) 486
TspDTI ATGAA 2 cut(s) 330, 427
TspGWI ACGGA 1 cut(s) 251
Vha464I CTTAAG 1 cut(s) 871
VpaK11BI GGWCC 1 cut(s) 467
XbaI TCTAGA 1 cut(s) 140
XceI RCATGY 1 cut(s) 892
XcmI CCANNNNNNNNNTGG 1 cut(s) 539
XmnI GAANNNNTTC 1 cut(s) 703
XspI CTAG 3 cut(s) 87, 141, 491
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.