MD16G1230800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0010950
Physical Location & Seq
Reverse (-)
23645029 .. 23647907
2879 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1230800.v1.1.491

Sequence Viewer

Length: 879 bp
ATGGAGAGCAGTGTCCTCCTTACAGCACAGCCTCATCCATGGCGACCCAATTATTCCTTTCACTTTATCTGTCAAAACCCACTTCCTTCCTCCCATTTAAGTCGCTCCTATGCTCCTAGCTACCGTCGCTGGGACTCCAATGCCGAAACCATTCGTTCCCAAAGATTCGGTGTCAATTTCAGAGACAAAGGCAACGGAGAAAAAGACGAAGACGATGAAGAAGACGAAGAAGATTACGAGTACAGTAGAAGGGAAGGGAAGAAGAAAAGGCGGTGGTGGTCGGACGAGTCGTCGGAGATGGAGGAGGAAGGCTCGGGTGGAATCTTGGAGGATGCCATTGATAGTGTCTGGATTTTTAAGGTGTTCAAATCGTATGGTTGGGCATTTCCAGCTATTATCATATCTTTGCTGCTGTCTACGGGTCCGAAAGCTTTCATAATGGCGTTAGCACTCCCCCTTGTTCAGTCAGCATTTTCACTGTTATATGAGAAGTTATGGGGAGGGACACAAAGTAGGCCGAAACGCAAGTCCAGGACCAGGAGGAGAAGGAAACCTTTTGCCAGCACTGTAGGTAACGCTAGAACCAAAGAAAAAGAAAAATATGATGAAGAGCAGGAAACTAGTGACAAAAGAATGGGTTATCAATCTTGGGTAGTTGGAAATGATGCTTCAGCTGATAGTAGTGGTCGAGATGCATCGAGTTTAGGCGGATGGGATGATCTTGAAAGAATGGAATCTGCACGGAGGCAATCTCAAAGAAAGCCGATGGGGAAGGGTAAGTTGAGTCGGAGAGAAAGAAATAGTGACACACCTCTCCTGTTAAGATTGTTGATTGCTGTTTTCCCATTTTTGGGTACATGGACAAAGATGTTTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

293

Amino Acids

33.83

Weight (kDa)

9.24

Isoelectric Point (pI)

66.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010950)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G33250 AT3G04310 AT3G04310
fragaria_vesca FvH4_4g05190 FvH4_4g05190
malus_domestica MD13G1225800.v1.1 MD16G1230800.v1.1
prunus_persica Prupe.1G052600_v2.0.a1
pyrus_communis pycom16g19270
rosa_chinensis RchiOBHm_Chr4g0396101
rosa_laevigata RLG00000009572
rosa_multiflora Rmu_sc0006325.1_g000019 Rmu_sc0007010.1_g000013 Rmu_sc0009634.1_g000010
rosa_roxburghii Rroxscaffold_5G00340900
rosa_rugosa Rorug03G0352600
rosa_samantha Rh4BG064200 Rh4CG070800 Rh4DG061200
rosa_wichuraiana Rw4G005330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 416
AciI CCGC 2 cut(s) 271, 708
AcuI CTGAAG 1 cut(s) 654
AfaI GTAC 2 cut(s) 242, 856
AfiI CCNNNNNNNGG 4 cut(s) 130, 537, 850, 851
AgsI TTSAA 2 cut(s) 367, 725
AhdI GACNNNNNGTC 1 cut(s) 289
AhlI ACTAGT 1 cut(s) 620
AjnI CCWGG 2 cut(s) 530, 536
AluBI AGCT 4 cut(s) 120, 392, 431, 674
AluI AGCT 4 cut(s) 120, 392, 431, 674
Alw26I GTCTC 1 cut(s) 177
Ama87I CYCGRG 1 cut(s) 313
AoxI GGCC 1 cut(s) 515
ApeKI GCWGC 1 cut(s) 409
ArsI GACNNNNNNTTYG 2 cut(s) 512, 544
Asp700I GAANNNNTTC 2 cut(s) 150, 431
AspS9I GGNCC 2 cut(s) 422, 534
AvaI CYCGRG 1 cut(s) 313
AvaII GGWCC 2 cut(s) 422, 534
BbsI GAAGAC 2 cut(s) 216, 228
BbvI GCAGC 1 cut(s) 396
BccI CCATC 3 cut(s) 292, 705, 760
BciT130I CCWGG 2 cut(s) 532, 538
BcoDI GTCTC 1 cut(s) 177
BcuI ACTAGT 1 cut(s) 620
BfaI CTAG 3 cut(s) 117, 579, 621
BfmI CTRYAG 1 cut(s) 567
BisI GCNGC 1 cut(s) 410
BlsI GCNGC 1 cut(s) 411
Bme1390I CCNGG 2 cut(s) 532, 538
Bme18I GGWCC 2 cut(s) 422, 534
BmeRI GACNNNNNGTC 1 cut(s) 289
BmeT110I CYCGRG 1 cut(s) 313
BmgT120I GGNCC 2 cut(s) 422, 534
BmiI GGNNCC 1 cut(s) 423
BmrFI CCNGG 2 cut(s) 532, 538
BmsI GCATC 4 cut(s) 322, 655, 682, 704
BpiI GAAGAC 2 cut(s) 216, 228
BplI GAGNNNNNCTC 4 cut(s) 296, 328, 736, 768
BsaJI CCNNGG 1 cut(s) 38
Bsc4I CCNNNNNNNGG 4 cut(s) 130, 537, 850, 851
BseBI CCWGG 2 cut(s) 532, 538
BseDI CCNNGG 1 cut(s) 38
BseGI GGATG 4 cut(s) 34, 337, 716, 721
BseLI CCNNNNNNNGG 4 cut(s) 130, 537, 850, 851
BseRI GAGGAG 2 cut(s) 317, 556
BseXI GCAGC 1 cut(s) 396
BseYI CCCAGC 1 cut(s) 129
BsgI GTGCAG 1 cut(s) 723
BshFI GGCC 1 cut(s) 517
BsiHKCI CYCGRG 1 cut(s) 313
BslFI GGGAC 2 cut(s) 146, 517
BslI CCNNNNNNNGG 4 cut(s) 130, 537, 850, 851
BsmAI GTCTC 1 cut(s) 177
BsmFI GGGAC 2 cut(s) 146, 517
BsnI GGCC 1 cut(s) 517
BsoBI CYCGRG 1 cut(s) 313
Bsp143I GATC 1 cut(s) 718
Bsp19I CCATGG 1 cut(s) 38
BspACI CCGC 2 cut(s) 271, 708
BspANI GGCC 1 cut(s) 517
BspLI GGNNCC 1 cut(s) 423
BspQI GCTCTTC 1 cut(s) 603
BssECI CCNNGG 1 cut(s) 38
BssMI GATC 1 cut(s) 718
BssT1I CCWWGG 1 cut(s) 38
Bst2UI CCWGG 2 cut(s) 532, 538
Bst4CI ACNGT 4 cut(s) 125, 245, 480, 568
Bst6I CTCTTC 1 cut(s) 603
BstC8I GCNNGC 1 cut(s) 562
BstDSI CCRYGG 1 cut(s) 38
BstF5I GGATG 4 cut(s) 34, 337, 716, 721
BstKTI GATC 1 cut(s) 721
BstMAI GTCTC 1 cut(s) 177
BstMBI GATC 1 cut(s) 718
BstMWI GCNNNNNNNGC 2 cut(s) 126, 389
BstNI CCWGG 2 cut(s) 532, 538
BstSCI CCNGG 2 cut(s) 530, 536
BstSFI CTRYAG 1 cut(s) 567
BstV1I GCAGC 1 cut(s) 396
BstV2I GAAGAC 2 cut(s) 216, 228
BsuRI GGCC 1 cut(s) 517
BtgI CCRYGG 1 cut(s) 38
BtsCI GGATG 4 cut(s) 34, 337, 716, 721
BtsI GCAGTG 1 cut(s) 16
BtsIMutI CAGTG 3 cut(s) 16, 476, 564
Cac8I GCNNGC 1 cut(s) 562
Cfr13I GGNCC 2 cut(s) 422, 534
Csp6I GTAC 2 cut(s) 241, 855
CviAII CATG 2 cut(s) 39, 858
CviJI RGCY 8 cut(s) 31, 120, 312, 392, 431, 517, 674, 763
CviKI_1 RGCY 8 cut(s) 31, 120, 312, 392, 431, 517, 674, 763
CviQI GTAC 2 cut(s) 241, 855
DpnI GATC 1 cut(s) 720
DpnII GATC 1 cut(s) 718
DriI GACNNNNNGTC 1 cut(s) 289
Eam1104I CTCTTC 1 cut(s) 603
Eam1105I GACNNNNNGTC 1 cut(s) 289
EarI CTCTTC 1 cut(s) 603
EciI GGCGGA 1 cut(s) 723
Eco130I CCWWGG 1 cut(s) 38
Eco47I GGWCC 2 cut(s) 422, 534
Eco57I CTGAAG 1 cut(s) 654
Eco88I CYCGRG 1 cut(s) 313
EcoRII CCWGG 2 cut(s) 530, 536
EcoT14I CCWWGG 1 cut(s) 38
EcoT22I ATGCAT 1 cut(s) 697
ErhI CCWWGG 1 cut(s) 38
FaeI CATG 2 cut(s) 42, 861
FalI AAGNNNNNCTT 2 cut(s) 538, 570
FaqI GGGAC 2 cut(s) 146, 517
FatI CATG 2 cut(s) 38, 857
FblI GTMKAC 1 cut(s) 416
Fnu4HI GCNGC 1 cut(s) 410
FokI GGATG 4 cut(s) 21, 344, 723, 728
Fsp4HI GCNGC 1 cut(s) 410
FspBI CTAG 3 cut(s) 117, 579, 621
GluI GCNGC 1 cut(s) 410
GsaI CCCAGC 1 cut(s) 133
HaeIII GGCC 1 cut(s) 517
Hin1II CATG 2 cut(s) 42, 861
HindIII AAGCTT 1 cut(s) 429
HinfI GANTC 6 cut(s) 134, 165, 287, 321, 734, 784
Hpy166II GTNNAC 1 cut(s) 417
Hpy188I TCNGA 5 cut(s) 182, 283, 295, 426, 789
Hpy188III TCNNGA 3 cut(s) 349, 689, 722
Hpy8I GTNNAC 1 cut(s) 417
Hpy99I CGWCG 2 cut(s) 129, 295
HpyAV CCTTC 6 cut(s) 96, 243, 248, 302, 540, 766
HpyCH4III ACNGT 4 cut(s) 125, 245, 480, 568
HpyCH4V TGCA 2 cut(s) 695, 740
HpyF10VI GCNNNNNNNGC 2 cut(s) 126, 389
Hsp92II CATG 2 cut(s) 42, 861
Kzo9I GATC 1 cut(s) 718
LguI GCTCTTC 1 cut(s) 603
LmnI GCTCC 2 cut(s) 110, 118
Lsp1109I GCAGC 1 cut(s) 396
LweI GCATC 4 cut(s) 322, 655, 682, 704
MaeI CTAG 3 cut(s) 117, 579, 621
MaeIII GTNAC 3 cut(s) 572, 623, 803
MalI GATC 1 cut(s) 720
MboI GATC 1 cut(s) 718
MboII GAAGA 8 cut(s) 221, 230, 233, 239, 242, 271, 274, 620
MluCI AATT 2 cut(s) 49, 175
MlyI GAGTC 3 cut(s) 128, 296, 793
MmeI TCCRAC 4 cut(s) 261, 273, 637, 767
Mph1103I ATGCAT 1 cut(s) 697
MroXI GAANNNNTTC 2 cut(s) 150, 431
MseI TTAA 3 cut(s) 98, 357, 821
MspA1I CMGCKG 1 cut(s) 674
MspR9I CCNGG 2 cut(s) 532, 538
MvaI CCWGG 2 cut(s) 532, 538
MwoI GCNNNNNNNGC 2 cut(s) 126, 389
NcoI CCATGG 1 cut(s) 38
NdeII GATC 1 cut(s) 718
NlaIII CATG 2 cut(s) 42, 861
NlaIV GGNNCC 1 cut(s) 423
NmuCI GTSAC 2 cut(s) 623, 803
NsiI ATGCAT 1 cut(s) 697
PciSI GCTCTTC 1 cut(s) 603
PcsI WCGNNNNNNNCGW 1 cut(s) 287
PdmI GAANNNNTTC 2 cut(s) 150, 431
PfeI GAWTC 3 cut(s) 165, 321, 734
PfoI TCCNGGA 1 cut(s) 530
PkrI GCNGC 1 cut(s) 411
PleI GAGTC 3 cut(s) 128, 295, 792
PpsI GAGTC 3 cut(s) 128, 295, 792
Psp6I CCWGG 2 cut(s) 530, 536
PspFI CCCAGC 1 cut(s) 129
PspGI CCWGG 2 cut(s) 530, 536
PspN4I GGNNCC 1 cut(s) 423
PspPI GGNCC 2 cut(s) 422, 534
PvuII CAGCTG 1 cut(s) 674
RsaI GTAC 2 cut(s) 242, 856
RsaNI GTAC 2 cut(s) 241, 855
SapI GCTCTTC 1 cut(s) 603
SaqAI TTAA 3 cut(s) 98, 357, 821
SatI GCNGC 1 cut(s) 410
Sau3AI GATC 1 cut(s) 718
Sau96I GGNCC 2 cut(s) 422, 534
SchI GAGTC 3 cut(s) 128, 296, 793
ScrFI CCNGG 2 cut(s) 532, 538
SetI ASST 8 cut(s) 122, 363, 394, 433, 556, 574, 676, 814
SfaNI GCATC 4 cut(s) 322, 655, 682, 704
SfcI CTRYAG 1 cut(s) 567
SinI GGWCC 2 cut(s) 422, 534
SpeI ACTAGT 1 cut(s) 620
Sse9I AATT 2 cut(s) 49, 175
SsiI CCGC 2 cut(s) 271, 708
SspMI CTAG 3 cut(s) 117, 579, 621
StyD4I CCNGG 2 cut(s) 530, 536
StyI CCWWGG 1 cut(s) 38
TaaI ACNGT 4 cut(s) 125, 245, 480, 568
TaqI TCGA 2 cut(s) 688, 698
TasI AATT 2 cut(s) 49, 175
TatI WGTACW 1 cut(s) 240
TfiI GAWTC 3 cut(s) 165, 321, 734
Tru1I TTAA 3 cut(s) 98, 357, 821
Tru9I TTAA 3 cut(s) 98, 357, 821
TscAI CASTG 3 cut(s) 16, 483, 571
TseFI GTSAC 2 cut(s) 623, 803
TseI GCWGC 1 cut(s) 409
Tsp45I GTSAC 2 cut(s) 623, 803
TspDTI ATGAA 3 cut(s) 231, 424, 621
TspGWI ACGGA 2 cut(s) 210, 757
TspRI CASTG 3 cut(s) 16, 483, 571
VpaK11BI GGWCC 2 cut(s) 422, 534
XmiI GTMKAC 1 cut(s) 416
XmnI GAANNNNTTC 2 cut(s) 150, 431
XspI CTAG 3 cut(s) 117, 579, 621
Zsp2I ATGCAT 1 cut(s) 697
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.