MD13G1225800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0010950
Physical Location & Seq
Reverse (-)
22066198 .. 22068465
2268 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1225800.v1.1.491

Sequence Viewer

Length: 882 bp
ATGGAGGGCGGTGTCCTCCCTCTCGCTGCACAGCCTCATCCATGGCGGCCCAGATATTCCTTTCACTTCATCTTTCAAAACCCACTTCCTTCGACCCACTTGAGTCGCTCCTACGCTCCTAGCTACCGTCGCTGGGACTCCAATGCCGAAACCATTCGTTCCCAAAGATTCGGTTTTAATTTCAGAGACAAAGGCAAAAGAGAAGAAGATGCCGGCGATGACGAAGAAGAAGAAGATTACGAGTACAGTAGAAGTAAAGGGACGAATAAAAGAAGGTGGTGGTCGGACGAGTCGTCGGAGATGGAGGAGGAAAGCTCCGGTGGAATCTTGGAGGATGCCATTGATAGTTTCTGGATATTGAAGGTGTTTAAATCCTATGGATGGGCATTTCCAGCTATTATTGCATCTTTGCTGCTGTCTACGGGTCCAAAAGCTTTCCTCATGGCGTTAGCACTCCCCCTTGGTCAGTCGGCATTTTCACTGTTATTTGAGAAGTTGTGGGGACGGACAAGGAGTAGGCCGAAACACAAGTCCAGGACAAGGAGGAGAAGGAAACCTTTTGCCAGCAGTTTTGGTAATGCTAAAACAGATGACAAAGAAAGATATGTTGAGGACCAGGAAACTAGTGACAGAAGCATGGGTTATCAATCTTGGGTAGTCGGAAATGATGCTTCAGCTGATGATAGTGGTCGGGAGGCATCGAGTTTAGGTGGATGGGATGATCTTGAAAGAATGGAATCTGCACGGAGGCAATCTCGAAGAAAGCCAATGGGGAAGGGTAAGTTGAGTCGGAGAGAAAGAAATAGCGACACGCCACTGCTGTTAAGATTGTTGATTGCTGTTTTCCCATTTTTGGGTACATGGACAAAGATGTTTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

294

Amino Acids

33.93

Weight (kDa)

9.49

Isoelectric Point (pI)

70.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010950)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G33250 AT3G04310 AT3G04310
fragaria_vesca FvH4_4g05190 FvH4_4g05190
malus_domestica MD13G1225800.v1.1 MD16G1230800.v1.1
prunus_persica Prupe.1G052600_v2.0.a1
pyrus_communis pycom16g19270
rosa_chinensis RchiOBHm_Chr4g0396101
rosa_laevigata RLG00000009572
rosa_multiflora Rmu_sc0006325.1_g000019 Rmu_sc0007010.1_g000013 Rmu_sc0009634.1_g000010
rosa_roxburghii Rroxscaffold_5G00340900
rosa_rugosa Rorug03G0352600
rosa_samantha Rh4BG064200 Rh4CG070800 Rh4DG061200
rosa_wichuraiana Rw4G005330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 419
AciI CCGC 2 cut(s) 9, 46
AcuI CTGAAG 1 cut(s) 657
AfaI GTAC 2 cut(s) 245, 859
AfiI CCNNNNNNNGG 5 cut(s) 133, 381, 540, 853, 854
AgsI TTSAA 3 cut(s) 77, 361, 728
AhdI GACNNNNNGTC 1 cut(s) 292
AhlI ACTAGT 1 cut(s) 623
AjnI CCWGG 2 cut(s) 533, 615
AluBI AGCT 5 cut(s) 123, 315, 395, 434, 677
AluI AGCT 5 cut(s) 123, 315, 395, 434, 677
Alw26I GTCTC 1 cut(s) 180
AoxI GGCC 2 cut(s) 47, 518
ApeKI GCWGC 2 cut(s) 26, 412
ArsI GACNNNNNNTTYG 2 cut(s) 515, 547
Asp700I GAANNNNTTC 1 cut(s) 153
AspS9I GGNCC 3 cut(s) 48, 425, 613
AvaII GGWCC 2 cut(s) 425, 613
BbvI GCAGC 2 cut(s) 13, 399
BccI CCATC 3 cut(s) 295, 375, 708
BciT130I CCWGG 2 cut(s) 535, 617
BcoDI GTCTC 1 cut(s) 180
BcuI ACTAGT 1 cut(s) 623
BfaI CTAG 2 cut(s) 120, 624
BisI GCNGC 3 cut(s) 27, 47, 413
BlsI GCNGC 3 cut(s) 28, 48, 414
Bme1390I CCNGG 2 cut(s) 535, 617
Bme18I GGWCC 2 cut(s) 425, 613
BmeRI GACNNNNNGTC 1 cut(s) 292
BmgT120I GGNCC 3 cut(s) 48, 425, 613
BmiI GGNNCC 1 cut(s) 426
BmrFI CCNGG 2 cut(s) 535, 617
BmsI GCATC 5 cut(s) 199, 325, 413, 658, 707
BplI GAGNNNNNCTC 4 cut(s) 299, 331, 739, 771
BpuEI CTTGAG 1 cut(s) 121
BsaJI CCNNGG 2 cut(s) 41, 460
BsaWI WCCGGW 1 cut(s) 317
Bsc4I CCNNNNNNNGG 5 cut(s) 133, 381, 540, 853, 854
Bse118I RCCGGY 1 cut(s) 212
BseBI CCWGG 2 cut(s) 535, 617
BseDI CCNNGG 2 cut(s) 41, 460
BseGI GGATG 5 cut(s) 37, 340, 386, 719, 724
BseLI CCNNNNNNNGG 5 cut(s) 133, 381, 540, 853, 854
BseRI GAGGAG 2 cut(s) 320, 559
BseXI GCAGC 2 cut(s) 13, 399
BseYI CCCAGC 1 cut(s) 132
BsgI GTGCAG 2 cut(s) 12, 726
BshFI GGCC 2 cut(s) 49, 520
BsiSI CCGG 2 cut(s) 213, 318
BslFI GGGAC 3 cut(s) 149, 274, 516
BslI CCNNNNNNNGG 5 cut(s) 133, 381, 540, 853, 854
BsmAI GTCTC 1 cut(s) 180
BsmFI GGGAC 3 cut(s) 149, 274, 516
BsnI GGCC 2 cut(s) 49, 520
Bsp143I GATC 1 cut(s) 721
Bsp19I CCATGG 1 cut(s) 41
BspACI CCGC 2 cut(s) 9, 46
BspANI GGCC 2 cut(s) 49, 520
BspLI GGNNCC 1 cut(s) 426
BsrFI RCCGGY 1 cut(s) 212
BssAI RCCGGY 1 cut(s) 212
BssECI CCNNGG 2 cut(s) 41, 460
BssMI GATC 1 cut(s) 721
BssT1I CCWWGG 2 cut(s) 41, 460
Bst2UI CCWGG 2 cut(s) 535, 617
Bst4CI ACNGT 3 cut(s) 128, 248, 483
BstC8I GCNNGC 2 cut(s) 214, 565
BstDSI CCRYGG 1 cut(s) 41
BstF5I GGATG 5 cut(s) 37, 340, 386, 719, 724
BstKTI GATC 1 cut(s) 724
BstMAI GTCTC 1 cut(s) 180
BstMBI GATC 1 cut(s) 721
BstMWI GCNNNNNNNGC 3 cut(s) 129, 392, 401
BstNI CCWGG 2 cut(s) 535, 617
BstSCI CCNGG 2 cut(s) 533, 615
BstV1I GCAGC 2 cut(s) 13, 399
BsuRI GGCC 2 cut(s) 49, 520
BtgI CCRYGG 1 cut(s) 41
BtgZI GCGATG 1 cut(s) 231
BtsCI GGATG 5 cut(s) 37, 340, 386, 719, 724
BtsI GCAGTG 1 cut(s) 815
BtsIMutI CAGTG 2 cut(s) 479, 815
Cac8I GCNNGC 2 cut(s) 214, 565
Cfr10I RCCGGY 1 cut(s) 212
Cfr13I GGNCC 3 cut(s) 48, 425, 613
Csp6I GTAC 2 cut(s) 244, 858
CviAII CATG 4 cut(s) 42, 442, 637, 861
CviJI RGCY 9 cut(s) 34, 49, 123, 315, 395, 434, 520, 677, 766
CviKI_1 RGCY 9 cut(s) 34, 49, 123, 315, 395, 434, 520, 677, 766
CviQI GTAC 2 cut(s) 244, 858
DpnI GATC 1 cut(s) 723
DpnII GATC 1 cut(s) 721
DraI TTTAAA 1 cut(s) 370
DriI GACNNNNNGTC 1 cut(s) 292
Eam1105I GACNNNNNGTC 1 cut(s) 292
Eco130I CCWWGG 2 cut(s) 41, 460
Eco47I GGWCC 2 cut(s) 425, 613
Eco57I CTGAAG 1 cut(s) 657
EcoRII CCWGG 2 cut(s) 533, 615
EcoT14I CCWWGG 2 cut(s) 41, 460
ErhI CCWWGG 2 cut(s) 41, 460
FaeI CATG 4 cut(s) 45, 445, 640, 864
FaiI YATR 6 cut(s) 43, 378, 443, 606, 638, 862
FalI AAGNNNNNCTT 2 cut(s) 541, 573
FaqI GGGAC 3 cut(s) 149, 274, 516
FatI CATG 4 cut(s) 41, 441, 636, 860
FblI GTMKAC 1 cut(s) 419
Fnu4HI GCNGC 3 cut(s) 27, 47, 413
FokI GGATG 5 cut(s) 24, 347, 393, 726, 731
Fsp4HI GCNGC 3 cut(s) 27, 47, 413
FspBI CTAG 2 cut(s) 120, 624
GluI GCNGC 3 cut(s) 27, 47, 413
GsaI CCCAGC 1 cut(s) 136
HaeIII GGCC 2 cut(s) 49, 520
HapII CCGG 2 cut(s) 213, 318
Hin1II CATG 4 cut(s) 45, 445, 640, 864
HindIII AAGCTT 1 cut(s) 432
HinfI GANTC 7 cut(s) 103, 137, 168, 290, 324, 737, 787
HpaII CCGG 2 cut(s) 213, 318
Hpy166II GTNNAC 1 cut(s) 420
Hpy188I TCNGA 5 cut(s) 185, 286, 298, 662, 792
Hpy188III TCNNGA 4 cut(s) 352, 692, 725, 756
Hpy8I GTNNAC 1 cut(s) 420
Hpy99I CGWCG 2 cut(s) 132, 298
HpyAV CCTTC 5 cut(s) 99, 267, 355, 543, 769
HpyCH4III ACNGT 3 cut(s) 128, 248, 483
HpyCH4V TGCA 3 cut(s) 29, 404, 743
HpyF10VI GCNNNNNNNGC 3 cut(s) 129, 392, 401
Hsp92II CATG 4 cut(s) 45, 445, 640, 864
KroI GCCGGC 1 cut(s) 212
KroNI GCCGGC 1 cut(s) 214
Kzo9I GATC 1 cut(s) 721
LmnI GCTCC 3 cut(s) 113, 121, 320
Lsp1109I GCAGC 2 cut(s) 13, 399
LweI GCATC 5 cut(s) 199, 325, 413, 658, 707
MaeI CTAG 2 cut(s) 120, 624
MaeIII GTNAC 1 cut(s) 626
MalI GATC 1 cut(s) 723
MboI GATC 1 cut(s) 721
MboII GAAGA 7 cut(s) 215, 218, 236, 239, 242, 245, 771
MluCI AATT 1 cut(s) 178
MlyI GAGTC 4 cut(s) 112, 131, 299, 796
MmeI TCCRAC 4 cut(s) 264, 276, 640, 770
MroNI GCCGGC 1 cut(s) 212
MroXI GAANNNNTTC 1 cut(s) 153
MseI TTAA 3 cut(s) 177, 369, 824
MspA1I CMGCKG 1 cut(s) 677
MspI CCGG 2 cut(s) 213, 318
MspR9I CCNGG 2 cut(s) 535, 617
MvaI CCWGG 2 cut(s) 535, 617
MwoI GCNNNNNNNGC 3 cut(s) 129, 392, 401
NaeI GCCGGC 1 cut(s) 214
NcoI CCATGG 1 cut(s) 41
NdeII GATC 1 cut(s) 721
NgoMIV GCCGGC 1 cut(s) 212
NlaIII CATG 4 cut(s) 45, 445, 640, 864
NlaIV GGNNCC 1 cut(s) 426
NmuCI GTSAC 1 cut(s) 626
PcsI WCGNNNNNNNCGW 1 cut(s) 290
PdiI GCCGGC 1 cut(s) 214
PdmI GAANNNNTTC 1 cut(s) 153
PfeI GAWTC 3 cut(s) 168, 324, 737
PfoI TCCNGGA 1 cut(s) 533
PkrI GCNGC 3 cut(s) 28, 48, 414
PleI GAGTC 4 cut(s) 111, 131, 298, 795
PpsI GAGTC 4 cut(s) 111, 131, 298, 795
Psp6I CCWGG 2 cut(s) 533, 615
PspFI CCCAGC 1 cut(s) 132
PspGI CCWGG 2 cut(s) 533, 615
PspN4I GGNNCC 1 cut(s) 426
PspPI GGNCC 3 cut(s) 48, 425, 613
PvuII CAGCTG 1 cut(s) 677
RsaI GTAC 2 cut(s) 245, 859
RsaNI GTAC 2 cut(s) 244, 858
SaqAI TTAA 3 cut(s) 177, 369, 824
SatI GCNGC 3 cut(s) 27, 47, 413
Sau3AI GATC 1 cut(s) 721
Sau96I GGNCC 3 cut(s) 48, 425, 613
SchI GAGTC 4 cut(s) 112, 131, 299, 796
ScrFI CCNGG 2 cut(s) 535, 617
SetI ASST 9 cut(s) 125, 278, 317, 366, 397, 436, 559, 679, 712
SfaNI GCATC 5 cut(s) 199, 325, 413, 658, 707
SinI GGWCC 2 cut(s) 425, 613
SmlI CTYRAG 1 cut(s) 100
SmoI CTYRAG 1 cut(s) 100
SpeI ACTAGT 1 cut(s) 623
Sse9I AATT 1 cut(s) 178
SsiI CCGC 2 cut(s) 9, 46
SspMI CTAG 2 cut(s) 120, 624
StyD4I CCNGG 2 cut(s) 533, 615
StyI CCWWGG 2 cut(s) 41, 460
TaaI ACNGT 3 cut(s) 128, 248, 483
TaqI TCGA 3 cut(s) 92, 701, 757
TasI AATT 1 cut(s) 178
TatI WGTACW 1 cut(s) 243
TauI GCSGC 1 cut(s) 49
TfiI GAWTC 3 cut(s) 168, 324, 737
Tru1I TTAA 3 cut(s) 177, 369, 824
Tru9I TTAA 3 cut(s) 177, 369, 824
TscAI CASTG 2 cut(s) 486, 822
TseFI GTSAC 1 cut(s) 626
TseI GCWGC 2 cut(s) 26, 412
Tsp45I GTSAC 1 cut(s) 626
TspDTI ATGAA 1 cut(s) 58
TspGWI ACGGA 2 cut(s) 520, 760
TspRI CASTG 2 cut(s) 486, 822
VpaK11BI GGWCC 2 cut(s) 425, 613
XmiI GTMKAC 1 cut(s) 419
XmnI GAANNNNTTC 1 cut(s) 153
XspI CTAG 2 cut(s) 120, 624
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.