Rw4G005330

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0010950
Physical Location & Seq
Reverse (-)
10762876 .. 10764912
2037 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G005330.1

Sequence Viewer

Length: 888 bp
ATGGAGAGCAGTGTCCTGGTCCTCACACACCCTCATCCATGGCGACCCAGATATTCCCTTCACTCTCTTTTTCAAAACCCACGTCCTCCTTCCTCTCATCTACTTCGCTCTTATGCTACTAAGTACCGCCGCTGGGATTCCAATGCCGAAACCATTCGCTCTAAAACATTCGGTTTTAGTTTCAGAGACAGACGAAACGGAGATGAAGAAGATGACGATGACTATGAGGAAGAAGATGATTATGGTTACAATACAAGTAAAGGGAAGAAGAAGAAGAAGAATAAGAGGCGGTGGTGGTCAGATGGGCCCTCTGAGATGGAGGAAAAACCTGTTGGAGTCTTGGAGGAAGCCATTGACAGTGTCTGGATTTTCAAGGTGTTCAGATCTTATGGTTGGACATTACCATTTATTGTCATATCTCTGCTGCTATCTACGGGTCCAAAAGCTTTCCTCATGGCATTAGCACTTCCCCTTGGTCAGTCTGCCCTTTCACTGGCAATTGAGAAGTTATGGGGAAGGACGCAAAAAAGGCCAAAACGCAAGTCCAGGATGAGAAGGAAACCGTCTGCCACCAGTGAAGCTAATGTTAAAATGGACGAAGAAGAAACATATGATGAAGATCAGGAAACTAATAATAAAAAGATGGATTACCAATCTTGGGTTGTTGGGAATGATGTTTCAGTTGGTAATAGTGGCGAAGATGCGTCAAAATTAGGTGGATGGGATGATCTTGAAAGAATGGGATCTGAAAAGAGGCAATCTCGAAGAAAGTCTAGAGGGAAGGGTAAGTTGAGTCGGAGAGAAAGAATTAGTGATACACCTCTTCTGCTGAGGTTGTTGATTGCAGTTTTCCCTTTTTTGGGTTCATGGACAAAGATATTTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

295

Amino Acids

34.36

Weight (kDa)

9.6

Isoelectric Point (pI)

64.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010950)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G33250 AT3G04310 AT3G04310
fragaria_vesca FvH4_4g05190 FvH4_4g05190
malus_domestica MD13G1225800.v1.1 MD16G1230800.v1.1
prunus_persica Prupe.1G052600_v2.0.a1
pyrus_communis pycom16g19270
rosa_chinensis RchiOBHm_Chr4g0396101
rosa_laevigata RLG00000009572
rosa_multiflora Rmu_sc0006325.1_g000019 Rmu_sc0007010.1_g000013 Rmu_sc0009634.1_g000010
rosa_roxburghii Rroxscaffold_5G00340900
rosa_rugosa Rorug03G0352600
rosa_samantha Rh4BG064200 Rh4CG070800 Rh4DG061200
rosa_wichuraiana Rw4G005330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 127, 130, 289
AclWI GGATC 1 cut(s) 751
AfaI GTAC 1 cut(s) 125
AfiI CCNNNNNNNGG 5 cut(s) 133, 493, 658, 859, 860
AgsI TTSAA 3 cut(s) 74, 373, 734
AjiI CACGTC 1 cut(s) 83
AjnI CCWGG 2 cut(s) 15, 545
AjuI GAANNNNNNNTTGG 2 cut(s) 315, 347
AluBI AGCT 2 cut(s) 446, 581
AluI AGCT 2 cut(s) 446, 581
Alw26I GTCTC 1 cut(s) 180
AlwI GGATC 1 cut(s) 751
AlwNI CAGNNNCTG 1 cut(s) 363
AoxI GGCC 2 cut(s) 305, 530
ApaI GGGCCC 1 cut(s) 309
ApeKI GCWGC 1 cut(s) 424
ArsI GACNNNNNNTTYG 4 cut(s) 67, 99, 527, 559
Asp700I GAANNNNTTC 1 cut(s) 153
AspS9I GGNCC 4 cut(s) 19, 305, 306, 437
AvaII GGWCC 2 cut(s) 19, 437
BaeGI GKGCMC 1 cut(s) 309
BanII GRGCYC 1 cut(s) 309
BbvCI CCTCAGC 1 cut(s) 830
BbvI GCAGC 1 cut(s) 411
BccI CCATC 4 cut(s) 296, 310, 637, 714
BciT130I CCWGG 2 cut(s) 17, 547
BcoDI GTCTC 1 cut(s) 180
BfaI CTAG 1 cut(s) 774
BglII AGATCT 1 cut(s) 383
BisI GCNGC 2 cut(s) 130, 425
BlsI GCNGC 2 cut(s) 131, 426
Bme1390I CCNGG 2 cut(s) 17, 547
Bme18I GGWCC 2 cut(s) 19, 437
BmgBI CACGTC 1 cut(s) 83
BmgT120I GGNCC 4 cut(s) 19, 305, 306, 437
BmiI GGNNCC 2 cut(s) 307, 438
BmrFI CCNGG 2 cut(s) 17, 547
BmsI GCATC 1 cut(s) 691
BplI GAGNNNNNCTC 2 cut(s) 745, 777
Bpu10I CCTNAGC 1 cut(s) 830
BsaBI GATNNNNATC 1 cut(s) 618
BsaJI CCNNGG 2 cut(s) 38, 472
Bsc4I CCNNNNNNNGG 5 cut(s) 133, 493, 658, 859, 860
Bse1I ACTGG 2 cut(s) 498, 573
Bse8I GATNNNNATC 1 cut(s) 618
BseBI CCWGG 2 cut(s) 17, 547
BseDI CCNNGG 2 cut(s) 38, 472
BseGI GGATG 4 cut(s) 34, 555, 725, 730
BseJI GATNNNNATC 1 cut(s) 618
BseLI CCNNNNNNNGG 5 cut(s) 133, 493, 658, 859, 860
BseMII CTCAG 2 cut(s) 303, 821
BseNI ACTGG 2 cut(s) 498, 573
BseSI GKGCMC 1 cut(s) 309
BseXI GCAGC 1 cut(s) 411
BseYI CCCAGC 1 cut(s) 132
BshFI GGCC 2 cut(s) 307, 532
BslI CCNNNNNNNGG 5 cut(s) 133, 493, 658, 859, 860
BsmAI GTCTC 1 cut(s) 180
BsnI GGCC 2 cut(s) 307, 532
Bsp120I GGGCCC 1 cut(s) 305
Bsp1286I GDGCHC 1 cut(s) 309
Bsp143I GATC 4 cut(s) 383, 619, 727, 743
Bsp19I CCATGG 1 cut(s) 38
BspACI CCGC 3 cut(s) 127, 130, 289
BspANI GGCC 2 cut(s) 307, 532
BspCNI CTCAG 2 cut(s) 304, 822
BspLI GGNNCC 2 cut(s) 307, 438
BspPI GGATC 1 cut(s) 751
BsrI ACTGG 2 cut(s) 498, 573
BssECI CCNNGG 2 cut(s) 38, 472
BssMI GATC 4 cut(s) 383, 619, 727, 743
BssT1I CCWWGG 2 cut(s) 38, 472
Bst2UI CCWGG 2 cut(s) 17, 547
Bst4CI ACNGT 2 cut(s) 359, 564
Bst6I CTCTTC 1 cut(s) 828
BstDEI CTNAG 3 cut(s) 120, 312, 830
BstDSI CCRYGG 1 cut(s) 38
BstF5I GGATG 4 cut(s) 34, 555, 725, 730
BstKTI GATC 4 cut(s) 386, 622, 730, 746
BstMAI GTCTC 1 cut(s) 180
BstMBI GATC 4 cut(s) 383, 619, 727, 743
BstMWI GCNNNNNNNGC 1 cut(s) 529
BstNI CCWGG 2 cut(s) 17, 547
BstSCI CCNGG 2 cut(s) 15, 545
BstSLI GKGCMC 1 cut(s) 309
BstV1I GCAGC 1 cut(s) 411
BstX2I RGATCY 2 cut(s) 383, 743
BstYI RGATCY 2 cut(s) 383, 743
BsuRI GGCC 2 cut(s) 307, 532
BtgI CCRYGG 1 cut(s) 38
BtrI CACGTC 1 cut(s) 83
BtsCI GGATG 4 cut(s) 34, 555, 725, 730
BtsI GCAGTG 1 cut(s) 16
BtsIMutI CAGTG 4 cut(s) 16, 364, 491, 580
CaiI CAGNNNCTG 1 cut(s) 363
Cfr13I GGNCC 4 cut(s) 19, 305, 306, 437
CseI GACGC 2 cut(s) 529, 693
Csp6I GTAC 1 cut(s) 124
CviAII CATG 3 cut(s) 39, 454, 867
CviJI RGCY 5 cut(s) 307, 350, 446, 532, 581
CviKI_1 RGCY 5 cut(s) 307, 350, 446, 532, 581
CviQI GTAC 1 cut(s) 124
DdeI CTNAG 3 cut(s) 120, 312, 830
DpnI GATC 4 cut(s) 385, 621, 729, 745
DpnII GATC 4 cut(s) 383, 619, 727, 743
Eam1104I CTCTTC 1 cut(s) 828
EarI CTCTTC 1 cut(s) 828
Eco130I CCWWGG 2 cut(s) 38, 472
Eco24I GRGCYC 1 cut(s) 309
Eco47I GGWCC 2 cut(s) 19, 437
EcoO109I RGGNCCY 1 cut(s) 306
EcoRII CCWGG 2 cut(s) 15, 545
EcoT14I CCWWGG 2 cut(s) 38, 472
EcoT38I GRGCYC 1 cut(s) 309
ErhI CCWWGG 2 cut(s) 38, 472
FaeI CATG 3 cut(s) 42, 457, 870
FatI CATG 3 cut(s) 38, 453, 866
FauNDI CATATG 1 cut(s) 610
Fnu4HI GCNGC 2 cut(s) 130, 425
FokI GGATG 4 cut(s) 21, 562, 732, 737
FriOI GRGCYC 1 cut(s) 309
Fsp4HI GCNGC 2 cut(s) 130, 425
FspBI CTAG 1 cut(s) 774
GluI GCNGC 2 cut(s) 130, 425
GsaI CCCAGC 1 cut(s) 136
HaeIII GGCC 2 cut(s) 307, 532
HgaI GACGC 2 cut(s) 529, 693
Hin1II CATG 3 cut(s) 42, 457, 870
HindIII AAGCTT 1 cut(s) 444
HinfI GANTC 3 cut(s) 137, 336, 793
Hpy188I TCNGA 6 cut(s) 185, 301, 313, 383, 748, 798
Hpy188III TCNNGA 5 cut(s) 364, 623, 731, 762, 774
HpyAV CCTTC 5 cut(s) 68, 99, 510, 549, 775
HpyCH4III ACNGT 2 cut(s) 359, 564
HpyCH4IV ACGT 1 cut(s) 82
HpyCH4V TGCA 1 cut(s) 845
HpyF10VI GCNNNNNNNGC 1 cut(s) 529
HpyF3I CTNAG 3 cut(s) 120, 312, 830
HpySE526I ACGT 1 cut(s) 82
Hsp92II CATG 3 cut(s) 42, 457, 870
Kzo9I GATC 4 cut(s) 383, 619, 727, 743
Lsp1109I GCAGC 1 cut(s) 411
LweI GCATC 1 cut(s) 691
MaeI CTAG 1 cut(s) 774
MaeII ACGT 1 cut(s) 82
MaeIII GTNAC 1 cut(s) 245
MalI GATC 4 cut(s) 385, 621, 729, 745
MboI GATC 4 cut(s) 383, 619, 727, 743
MfeI CAATTG 1 cut(s) 498
MflI RGATCY 2 cut(s) 383, 743
MhlI GDGCHC 1 cut(s) 309
MluCI AATT 3 cut(s) 498, 710, 807
MlyI GAGTC 2 cut(s) 345, 802
MmeI TCCRAC 3 cut(s) 313, 374, 776
MroXI GAANNNNTTC 1 cut(s) 153
MseI TTAA 1 cut(s) 588
MspA1I CMGCKG 1 cut(s) 132
MspR9I CCNGG 2 cut(s) 17, 547
MunI CAATTG 1 cut(s) 498
MvaI CCWGG 2 cut(s) 17, 547
MwoI GCNNNNNNNGC 1 cut(s) 529
NcoI CCATGG 1 cut(s) 38
NdeI CATATG 1 cut(s) 610
NdeII GATC 4 cut(s) 383, 619, 727, 743
NlaIII CATG 3 cut(s) 42, 457, 870
NlaIV GGNNCC 2 cut(s) 307, 438
PdmI GAANNNNTTC 1 cut(s) 153
PfeI GAWTC 1 cut(s) 137
PflFI GACNNNGTC 1 cut(s) 359
PfoI TCCNGGA 1 cut(s) 545
PkrI GCNGC 2 cut(s) 131, 426
PleI GAGTC 2 cut(s) 344, 801
PpsI GAGTC 2 cut(s) 344, 801
Psp6I CCWGG 2 cut(s) 15, 545
PspFI CCCAGC 1 cut(s) 132
PspGI CCWGG 2 cut(s) 15, 545
PspN4I GGNNCC 2 cut(s) 307, 438
PspOMI GGGCCC 1 cut(s) 305
PspPI GGNCC 4 cut(s) 19, 305, 306, 437
PstNI CAGNNNCTG 1 cut(s) 363
PsuI RGATCY 2 cut(s) 383, 743
PsyI GACNNNGTC 1 cut(s) 359
RsaI GTAC 1 cut(s) 125
RsaNI GTAC 1 cut(s) 124
SaqAI TTAA 1 cut(s) 588
SatI GCNGC 2 cut(s) 130, 425
Sau3AI GATC 4 cut(s) 383, 619, 727, 743
Sau96I GGNCC 4 cut(s) 19, 305, 306, 437
SchI GAGTC 2 cut(s) 345, 802
ScrFI CCNGG 2 cut(s) 17, 547
SduI GDGCHC 1 cut(s) 309
SetI ASST 8 cut(s) 85, 331, 378, 448, 583, 718, 823, 836
SfaNI GCATC 1 cut(s) 691
SinI GGWCC 2 cut(s) 19, 437
Sse9I AATT 3 cut(s) 498, 710, 807
SsiI CCGC 3 cut(s) 127, 130, 289
SspMI CTAG 1 cut(s) 774
StyD4I CCNGG 2 cut(s) 15, 545
StyI CCWWGG 2 cut(s) 38, 472
TaaI ACNGT 2 cut(s) 359, 564
TaiI ACGT 1 cut(s) 85
TaqI TCGA 1 cut(s) 763
TasI AATT 3 cut(s) 498, 710, 807
TauI GCSGC 1 cut(s) 132
TfiI GAWTC 1 cut(s) 137
Tru1I TTAA 1 cut(s) 588
Tru9I TTAA 1 cut(s) 588
TscAI CASTG 4 cut(s) 16, 364, 498, 580
TseI GCWGC 1 cut(s) 424
TspDTI ATGAA 3 cut(s) 219, 630, 855
TspGWI ACGGA 1 cut(s) 213
TspRI CASTG 4 cut(s) 16, 364, 498, 580
Tth111I GACNNNGTC 1 cut(s) 359
VpaK11BI GGWCC 2 cut(s) 19, 437
XbaI TCTAGA 1 cut(s) 773
XmnI GAANNNNTTC 1 cut(s) 153
XspI CTAG 1 cut(s) 774
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.