AT2G35500

Shikimate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0004803
Physical Location & Seq
Forward (+)
14913937 .. 14916668
2732 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G35500.1

Sequence Viewer

Length: 1164 bp
ATGGCTGCTTTTGCTTCTGGTTTGGCTATAATCTTCAACTCTCCTTCACTAAACCCTGTCACAACTCAAGCGACCTTCCTTTCTTCGAATCGGATTCGTTCCTCTCCTCGCGTTTTCAGTGGATTTCACAGCCTCCGACGTCGCGGCTTCCGGCGATTCTCTCAAAATGTTATCCCCGACCGGTTTAATAGCTTCTCATGTAATTGCTTATCCGCCGTATCCACCAGCACTATCGATTACGAGTTCACGGATGGTGGCAAAGAGGTGGAATTAAGATTGAGGTTAAAGACAGGTGAGATACTTTCTCCAAAGGATATATCTGTTGACGCAGATGGAACATCGTTAGCCGTTAAAGAAAAGCGGAATGGATTGCTGATTACACTTCTAGAGACTAACCATCTCTTTGAGAAGATTATGCCCTCTGAAACAATATGGTACATAGACGAAGATCAGTTGGTTGTGAATATGAAGAAGGTGGATGGGGAATTGAAGTGGCCTGATATTGTCGAGTCCTGGGAGTCTTTGACTGCAGGAATGATGCAACTTCTTAAAGGTGCATCAATCTACATTGTTGGAGATTCAACTGAAATCAACCAAAAAGTGTCTCGAGAGCTAGCCGTTGGCCTTGGGTACAGTCCTTTAGATTCAAAGGAATTGCTGGAAAGCTTTTCCAAGCAAACAATCGATTCTTGGATTCTTGCAGAAGGGCCGGATTCTGTAGCTGAAGCAGAGAGTTCGGTATTGGAAAGCTTGAGCAGCCATGTGCGTACTGTTGTCTCAACTTTGGGAGGTAAACATGGAGCTGCGGGGAGAGCTGATCAGTGGAGACATCTTTACTCCGGATTTACCGTTTGGGTATCACAGACTGAAGCCACAGATGAAGAGTCAGCTAAAGAAGAAGCGAGGAGAAGTAAGCAAGAGAGGGAGATTGGGTATTCAAACGCCGACGTTGTGGTGAAGCTCCAAGGTTGGGACCCGACGCATGCAAAGAGCGTGGCTCAGGCTTCCTTGAGTGCACTTAAGCAACTGATCATCTCAGACAAGGGACTTCCAGGTAAGAAGAGTTTGTACATACGCTTGGGATGCCGTGGCGATTGGCCCAATATAAAACCGCCGGGATGGGATCCATCGTCTGACACTGGACCTCATCCACAGTTTACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000287 GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046872
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CS SKI

Protein Analysis

387

Amino Acids

42.69

Weight (kDa)

5.9

Isoelectric Point (pI)

40.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 142
AccII CGCG 2 cut(s) 111, 144
AccIII TCCGGA 1 cut(s) 839
AciI CCGC 5 cut(s) 144, 213, 361, 806, 1112
AclWI GGATC 2 cut(s) 1118, 1131
AcuI CTGAAG 2 cut(s) 744, 888
AcyI GRCGYC 1 cut(s) 139
AfaI GTAC 4 cut(s) 437, 632, 769, 1070
AfiI CCNNNNNNNGG 1 cut(s) 970
AflII CTTAAG 1 cut(s) 1019
AgeI ACCGGT 1 cut(s) 180
AgsI TTSAA 5 cut(s) 37, 490, 582, 648, 939
AjnI CCWGG 2 cut(s) 512, 1051
AluBI AGCT 9 cut(s) 192, 613, 666, 722, 750, 803, 815, 890, 961
AluI AGCT 9 cut(s) 192, 613, 666, 722, 750, 803, 815, 890, 961
Alw21I GWGCWC 1 cut(s) 1018
Alw26I GTCTC 4 cut(s) 383, 609, 781, 820
Alw44I GTGCAC 1 cut(s) 1014
AlwI GGATC 2 cut(s) 1118, 1131
Ama87I CYCGRG 1 cut(s) 606
Aor13HI TCCGGA 1 cut(s) 839
AoxI GGCC 4 cut(s) 494, 622, 707, 1097
ApaLI GTGCAC 1 cut(s) 1014
ApeKI GCWGC 3 cut(s) 5, 756, 803
AsiGI ACCGGT 1 cut(s) 180
AspS9I GGNCC 4 cut(s) 707, 973, 1098, 1142
AsuC2I CCSGG 1 cut(s) 1116
AsuHPI GGTGA 2 cut(s) 305, 967
AsuII TTCGAA 1 cut(s) 86
AsuNHI GCTAGC 1 cut(s) 613
AvaI CYCGRG 1 cut(s) 606
AvaII GGWCC 2 cut(s) 973, 1142
BaeGI GKGCMC 1 cut(s) 1018
BamHI GGATCC 1 cut(s) 1123
BarI GAAGNNNNNNTAC 2 cut(s) 1052, 1084
Bbv12I GWGCWC 1 cut(s) 1018
BbvI GCAGC 2 cut(s) 768, 790
BccI CCATC 6 cut(s) 245, 326, 405, 473, 1113, 1135
BceAI ACGGC 4 cut(s) 200, 332, 602, 1071
BcgI CGANNNNNNTGC 2 cut(s) 717, 751
BciT130I CCWGG 2 cut(s) 514, 1053
BciVI GTATCC 1 cut(s) 229
BclI TGATCA 2 cut(s) 817, 1029
BcnI CCSGG 1 cut(s) 1116
BcoDI GTCTC 4 cut(s) 383, 609, 781, 820
BfaI CTAG 2 cut(s) 386, 614
BfmI CTRYAG 2 cut(s) 528, 717
BfrI CTTAAG 1 cut(s) 1019
BfuI GTATCC 1 cut(s) 229
BisI GCNGC 4 cut(s) 6, 145, 757, 804
BlsI GCNGC 4 cut(s) 7, 146, 758, 805
Bme1390I CCNGG 3 cut(s) 514, 1053, 1116
Bme18I GGWCC 2 cut(s) 973, 1142
BmeT110I CYCGRG 1 cut(s) 606
BmgT120I GGNCC 4 cut(s) 707, 973, 1098, 1142
BmiI GGNNCC 3 cut(s) 974, 975, 1125
BmrFI CCNGG 3 cut(s) 514, 1053, 1116
BmsI GCATC 3 cut(s) 528, 566, 1073
BmtI GCTAGC 1 cut(s) 617
BplI GAGNNNNNCTC 2 cut(s) 982, 1014
Bpu10I CCTNAGC 1 cut(s) 999
Bpu14I TTCGAA 1 cut(s) 86
BpuEI CTTGAG 3 cut(s) 51, 772, 1030
BpuMI CCSGG 1 cut(s) 1116
Bsa29I ATCGAT 2 cut(s) 234, 684
BsaHI GRCGYC 1 cut(s) 139
BsaJI CCNNGG 4 cut(s) 513, 625, 964, 1087
BsaWI WCCGGW 2 cut(s) 180, 839
BsaXI ACNNNNNCTCC 2 cut(s) 917, 947
Bsc4I CCNNNNNNNGG 1 cut(s) 970
Bse118I RCCGGY 1 cut(s) 180
Bse1I ACTGG 1 cut(s) 1144
BseAI TCCGGA 1 cut(s) 839
BseBI CCWGG 2 cut(s) 514, 1053
BseCI ATCGAT 2 cut(s) 234, 684
BseDI CCNNGG 4 cut(s) 513, 625, 964, 1087
BseGI GGATG 5 cut(s) 256, 484, 1088, 1124, 1147
BseLI CCNNNNNNNGG 1 cut(s) 970
BseMII CTCAG 2 cut(s) 1013, 1050
BseNI ACTGG 1 cut(s) 1144
BseRI GAGGAG 2 cut(s) 96, 919
BseSI GKGCMC 1 cut(s) 1018
BseXI GCAGC 2 cut(s) 768, 790
Bsh1236I CGCG 2 cut(s) 111, 144
Bsh1285I CGRYCG 1 cut(s) 181
BshFI GGCC 4 cut(s) 496, 624, 709, 1099
BshTI ACCGGT 1 cut(s) 180
BshVI ATCGAT 2 cut(s) 234, 684
BsiEI CGRYCG 1 cut(s) 181
BsiHKAI GWGCWC 1 cut(s) 1018
BsiHKCI CYCGRG 1 cut(s) 606
BsiSI CCGG 5 cut(s) 151, 181, 710, 840, 1115
BslFI GGGAC 2 cut(s) 986, 1059
BslI CCNNNNNNNGG 1 cut(s) 970
BsmAI GTCTC 4 cut(s) 383, 609, 781, 820
BsmFI GGGAC 2 cut(s) 986, 1059
BsnI GGCC 4 cut(s) 496, 624, 709, 1099
BsoBI CYCGRG 1 cut(s) 606
Bsp119I TTCGAA 1 cut(s) 86
Bsp1286I GDGCHC 1 cut(s) 1018
Bsp13I TCCGGA 1 cut(s) 839
Bsp1407I TGTACA 1 cut(s) 1068
Bsp143I GATC 4 cut(s) 448, 817, 1029, 1123
BspACI CCGC 5 cut(s) 144, 213, 361, 806, 1112
BspANI GGCC 4 cut(s) 496, 624, 709, 1099
BspCNI CTCAG 2 cut(s) 1012, 1049
BspDI ATCGAT 2 cut(s) 234, 684
BspEI TCCGGA 1 cut(s) 839
BspFNI CGCG 2 cut(s) 111, 144
BspLI GGNNCC 3 cut(s) 974, 975, 1125
BspMAI CTGCAG 1 cut(s) 532
BspOI GCTAGC 1 cut(s) 617
BspPI GGATC 2 cut(s) 1118, 1131
BspT104I TTCGAA 1 cut(s) 86
BspTI CTTAAG 1 cut(s) 1019
BsrFI RCCGGY 1 cut(s) 180
BsrGI TGTACA 1 cut(s) 1068
BsrI ACTGG 1 cut(s) 1144
BssAI RCCGGY 1 cut(s) 180
BssECI CCNNGG 4 cut(s) 513, 625, 964, 1087
BssMI GATC 4 cut(s) 448, 817, 1029, 1123
BssNI GRCGYC 1 cut(s) 139
BssT1I CCWWGG 2 cut(s) 625, 964
Bst2UI CCWGG 2 cut(s) 514, 1053
Bst4CI ACNGT 4 cut(s) 635, 772, 850, 1155
Bst6I CTCTTC 2 cut(s) 876, 1055
BstACI GRCGYC 1 cut(s) 139
BstAFI CTTAAG 1 cut(s) 1019
BstAUI TGTACA 1 cut(s) 1068
BstBI TTCGAA 1 cut(s) 86
BstC8I GCNNGC 2 cut(s) 615, 984
BstDEI CTNAG 2 cut(s) 999, 1036
BstDSI CCRYGG 1 cut(s) 1087
BstF5I GGATG 5 cut(s) 256, 484, 1088, 1124, 1147
BstFNI CGCG 2 cut(s) 111, 144
BstKTI GATC 4 cut(s) 451, 820, 1032, 1126
BstMAI GTCTC 4 cut(s) 383, 609, 781, 820
BstMBI GATC 4 cut(s) 448, 817, 1029, 1123
BstMCI CGRYCG 1 cut(s) 181
BstMWI GCNNNNNNNGC 4 cut(s) 11, 756, 812, 1083
BstNI CCWGG 2 cut(s) 514, 1053
BstNSI RCATGY 1 cut(s) 986
BstSCI CCNGG 3 cut(s) 512, 1051, 1114
BstSFI CTRYAG 2 cut(s) 528, 717
BstSLI GKGCMC 1 cut(s) 1018
BstUI CGCG 2 cut(s) 111, 144
BstV1I GCAGC 2 cut(s) 768, 790
BstX2I RGATCY 1 cut(s) 1123
BstYI RGATCY 1 cut(s) 1123
Bsu15I ATCGAT 2 cut(s) 234, 684
BsuI GTATCC 1 cut(s) 229
BsuRI GGCC 4 cut(s) 496, 624, 709, 1099
BsuTUI ATCGAT 2 cut(s) 234, 684
BtgI CCRYGG 1 cut(s) 1087
BtsCI GGATG 5 cut(s) 256, 484, 1088, 1124, 1147
BtsIMutI CAGTG 3 cut(s) 124, 827, 1137
Cac8I GCNNGC 2 cut(s) 615, 984
Cfr10I RCCGGY 1 cut(s) 180
Cfr13I GGNCC 4 cut(s) 707, 973, 1098, 1142
ClaI ATCGAT 2 cut(s) 234, 684
CseI GACGC 2 cut(s) 335, 988
Csp6I GTAC 4 cut(s) 436, 631, 768, 1069
CspAI ACCGGT 1 cut(s) 180
CspCI CAANNNNNGTGG 2 cut(s) 975, 1010
CviAII CATG 4 cut(s) 198, 761, 797, 983
CviQI GTAC 4 cut(s) 436, 631, 768, 1069
DdeI CTNAG 2 cut(s) 999, 1036
DpnI GATC 4 cut(s) 450, 819, 1031, 1125
DpnII GATC 4 cut(s) 448, 817, 1029, 1123
Eam1104I CTCTTC 2 cut(s) 876, 1055
EarI CTCTTC 2 cut(s) 876, 1055
EciI GGCGGA 1 cut(s) 202
Eco130I CCWWGG 2 cut(s) 625, 964
Eco47I GGWCC 2 cut(s) 973, 1142
Eco57I CTGAAG 2 cut(s) 744, 888
Eco88I CYCGRG 1 cut(s) 606
EcoO109I RGGNCCY 1 cut(s) 973
EcoRII CCWGG 2 cut(s) 512, 1051
EcoT14I CCWWGG 2 cut(s) 625, 964
ErhI CCWWGG 2 cut(s) 625, 964
FaeI CATG 4 cut(s) 201, 764, 800, 986
FaqI GGGAC 2 cut(s) 986, 1059
FatI CATG 4 cut(s) 197, 760, 796, 982
FauI CCCGC 1 cut(s) 799
FbaI TGATCA 2 cut(s) 817, 1029
Fnu4HI GCNGC 4 cut(s) 6, 145, 757, 804
FokI GGATG 5 cut(s) 263, 491, 1095, 1131, 1134
Fsp4HI GCNGC 4 cut(s) 6, 145, 757, 804
FspBI CTAG 2 cut(s) 386, 614
GluI GCNGC 4 cut(s) 6, 145, 757, 804
HaeIII GGCC 4 cut(s) 496, 624, 709, 1099
HapII CCGG 5 cut(s) 151, 181, 710, 840, 1115
HgaI GACGC 2 cut(s) 335, 988
Hin1I GRCGYC 1 cut(s) 139
Hin1II CATG 4 cut(s) 201, 764, 800, 986
HincII GTYRAC 1 cut(s) 325
HindII GTYRAC 1 cut(s) 325
HindIII AAGCTT 2 cut(s) 664, 748
HpaII CCGG 5 cut(s) 151, 181, 710, 840, 1115
HphI GGTGA 2 cut(s) 305, 967
Hpy166II GTNNAC 5 cut(s) 246, 325, 794, 1016, 1158
Hpy188I TCNGA 5 cut(s) 93, 137, 424, 1039, 1135
Hpy188III TCNNGA 4 cut(s) 386, 606, 608, 840
Hpy8I GTNNAC 5 cut(s) 246, 325, 794, 1016, 1158
Hpy99I CGWCG 4 cut(s) 141, 144, 950, 982
HpyAV CCTTC 4 cut(s) 54, 85, 466, 698
HpyCH4III ACNGT 4 cut(s) 635, 772, 850, 1155
HpyCH4IV ACGT 2 cut(s) 139, 948
HpyCH4V TGCA 6 cut(s) 530, 541, 557, 701, 986, 1016
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 756, 812, 1083
HpyF3I CTNAG 2 cut(s) 999, 1036
HpySE526I ACGT 2 cut(s) 139, 948
Hsp92I GRCGYC 1 cut(s) 139
Hsp92II CATG 4 cut(s) 201, 764, 800, 986
KflI GGGWCCC 1 cut(s) 973
Kpn2I TCCGGA 1 cut(s) 839
Ksp22I TGATCA 2 cut(s) 817, 1029
Kzo9I GATC 4 cut(s) 448, 817, 1029, 1123
LmnI GCTCC 2 cut(s) 800, 966
Lsp1109I GCAGC 2 cut(s) 768, 790
LweI GCATC 3 cut(s) 528, 566, 1073
MaeI CTAG 2 cut(s) 386, 614
MaeII ACGT 2 cut(s) 139, 948
MaeIII GTNAC 1 cut(s) 58
MalI GATC 4 cut(s) 450, 819, 1031, 1125
MboI GATC 4 cut(s) 448, 817, 1029, 1123
MboII GAAGA 8 cut(s) 25, 75, 421, 458, 481, 893, 908, 1072
MflI RGATCY 1 cut(s) 1123
MhlI GDGCHC 1 cut(s) 1018
MluCI AATT 4 cut(s) 202, 269, 485, 653
MlyI GAGTC 3 cut(s) 518, 527, 893
MmeI TCCRAC 2 cut(s) 160, 553
MroI TCCGGA 1 cut(s) 839
MseI TTAA 6 cut(s) 186, 272, 284, 351, 549, 1020
MspCI CTTAAG 1 cut(s) 1019
MspI CCGG 5 cut(s) 151, 181, 710, 840, 1115
MspR9I CCNGG 3 cut(s) 514, 1053, 1116
MvaI CCWGG 2 cut(s) 514, 1053
MvnI CGCG 2 cut(s) 111, 144
MwoI GCNNNNNNNGC 4 cut(s) 11, 756, 812, 1083
NciI CCSGG 1 cut(s) 1116
NdeII GATC 4 cut(s) 448, 817, 1029, 1123
NheI GCTAGC 1 cut(s) 613
NlaIII CATG 4 cut(s) 201, 764, 800, 986
NlaIV GGNNCC 3 cut(s) 974, 975, 1125
NmuCI GTSAC 1 cut(s) 58
NspI RCATGY 1 cut(s) 986
NspV TTCGAA 1 cut(s) 86
PaeI GCATGC 1 cut(s) 986
PaeR7I CTCGAG 1 cut(s) 606
PfeI GAWTC 8 cut(s) 88, 94, 156, 578, 644, 686, 694, 713
PinAI ACCGGT 1 cut(s) 180
PkrI GCNGC 4 cut(s) 7, 146, 758, 805
PleI GAGTC 3 cut(s) 517, 526, 892
PpsI GAGTC 3 cut(s) 517, 526, 892
PpuMI RGGWCCY 1 cut(s) 973
Psp5II RGGWCCY 1 cut(s) 973
Psp6I CCWGG 2 cut(s) 512, 1051
PspGI CCWGG 2 cut(s) 512, 1051
PspN4I GGNNCC 3 cut(s) 974, 975, 1125
PspPI GGNCC 4 cut(s) 707, 973, 1098, 1142
PspPPI RGGWCCY 1 cut(s) 973
PstI CTGCAG 1 cut(s) 532
PsuI RGATCY 1 cut(s) 1123
RsaI GTAC 4 cut(s) 437, 632, 769, 1070
RsaNI GTAC 4 cut(s) 436, 631, 768, 1069
SaqAI TTAA 6 cut(s) 186, 272, 284, 351, 549, 1020
SatI GCNGC 4 cut(s) 6, 145, 757, 804
Sau3AI GATC 4 cut(s) 448, 817, 1029, 1123
Sau96I GGNCC 4 cut(s) 707, 973, 1098, 1142
SchI GAGTC 3 cut(s) 518, 527, 893
ScrFI CCNGG 3 cut(s) 514, 1053, 1116
SduI GDGCHC 1 cut(s) 1018
SfaNI GCATC 3 cut(s) 528, 566, 1073
SfcI CTRYAG 2 cut(s) 528, 717
Sfr274I CTCGAG 1 cut(s) 606
SfuI TTCGAA 1 cut(s) 86
SinI GGWCC 2 cut(s) 973, 1142
SlaI CTCGAG 1 cut(s) 606
SmlI CTYRAG 5 cut(s) 66, 606, 751, 1009, 1019
SmoI CTYRAG 5 cut(s) 66, 606, 751, 1009, 1019
SphI GCATGC 1 cut(s) 986
Sse9I AATT 4 cut(s) 202, 269, 485, 653
SsiI CCGC 5 cut(s) 144, 213, 361, 806, 1112
SspMI CTAG 2 cut(s) 386, 614
StyD4I CCNGG 3 cut(s) 512, 1051, 1114
StyI CCWWGG 2 cut(s) 625, 964
TaaI ACNGT 4 cut(s) 635, 772, 850, 1155
TaiI ACGT 2 cut(s) 142, 951
TaqI TCGA 5 cut(s) 86, 234, 507, 607, 684
TasI AATT 4 cut(s) 202, 269, 485, 653
TatI WGTACW 1 cut(s) 1068
TauI GCSGC 1 cut(s) 147
TfiI GAWTC 8 cut(s) 88, 94, 156, 578, 644, 686, 694, 713
Tru1I TTAA 6 cut(s) 186, 272, 284, 351, 549, 1020
Tru9I TTAA 6 cut(s) 186, 272, 284, 351, 549, 1020
TscAI CASTG 3 cut(s) 124, 827, 1144
TseFI GTSAC 1 cut(s) 58
TseI GCWGC 3 cut(s) 5, 756, 803
Tsp45I GTSAC 1 cut(s) 58
TspDTI ATGAA 2 cut(s) 482, 894
TspGWI ACGGA 1 cut(s) 263
TspRI CASTG 3 cut(s) 124, 827, 1144
Vha464I CTTAAG 1 cut(s) 1019
VneI GTGCAC 1 cut(s) 1014
VpaK11BI GGWCC 2 cut(s) 973, 1142
XbaI TCTAGA 1 cut(s) 385
XceI RCATGY 1 cut(s) 986
XhoI CTCGAG 1 cut(s) 606
XspI CTAG 2 cut(s) 386, 614
ZraI GACGTC 1 cut(s) 140
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.