FvH4_5g23010
NAC Family

inactive shikimate kinase like 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0004803
Physical Location & Seq
Forward (+)
14379087 .. 14383083
3997 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g23010.t5

Sequence Viewer

Length: 1146 bp
ATGGCCGCTACCACCACCACTTCCACTGCTTTAATCAACAATTATTTGCAACCCTCTTCCCTCCAAAACCTCTCAAGACCCAAATGGGCAATCACCAATTACCTTTCCCTCCCACCAACAACTCCATCTCGTGTTGGCTGCTGCCGTTTAAAGACCCCCCTCCCTCGTGTCTTCTCCTCCCAAAGCTTCCCCACCGCCCCCGTTACCCTCAAGGACTACGAGTTTCTTGATGGTTCTACTGAGGTGGAATTGAGATTACAGCTCAGGGGACGAAACATCACAAGCTCCAGAGACATTTTTGTGGATGCAAATGGCTCGTCTTTAACAATAAAAGTACAGCACTCTGGGTCCTTTGTCACACTTATGGAAACTAATCAACTGTTTGATAAAATAAAGCCTGCTGAAACAATATGGTATATAGATGAAGATCAGCTAGTGATAAACTTGAAGAAGCATGATCCAGATTTAAAATGGCCTGACATTGTCGAGTCATGGGAGTCTTTAACTGCGGGATCTATGCAACTTTTGAAAGGAACATCAATTTACATTGTTGGGGATTCAACTGAAATTAACCAGAAAGTGGCTCAAGAACTTGCAGTTGGTCTGGGATATACGCCACTCACTACAAAGGAGTTGCTGGAAGCATATGCCAAGCAGAGTGTTGACTCATGGCTGCTTGCTGAAGGTTCTGAATCTGTAGTGGAAGCAGAAAGTGCTATATTACAGAGTTTAAGTAGTCATGCTAGGGCAGTCATTGCGACATTAGGAGGCCAGCACGGAGCTGCTGGAAGAGCTCATAAATGGCACCATCTTTACGCAGGATTTACTATCTGGCTGTCACAAACTGATGCTACAGATGAAGGTTCTGCAAAGGAAGAGGCCAGGAGTCATATCCAAGATGGTGGAGTAGCTTACTCAAATGCAGACGTTGTTGTCAAGCTACAGGGTTGGGATGCAGATCACGTTAAAACTGTTGCGCAGGCATGCTTGAGTGCTCTTAAACAGTTAGTTCTGTCAGACAAGAAGCTTCCAGGTAAGAAGAGCCTCTACATACGCTTGGGATGCCGAGGTGACTGGCCAAACATCAAGCCTCCTGGGTGGGATCCTTCAGCTGGAGATGATGCATCTCCAGGTAGATCTCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000287 GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046872
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CS SKI

Protein Analysis

382

Amino Acids

41.6

Weight (kDa)

6.07

Isoelectric Point (pI)

41.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SKI PF01202 194 - 281 7.3e-06 Shikimate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 932
Acc16I TGCGCA 1 cut(s) 978
AccB1I GGYRCC 1 cut(s) 804
AccB7I CCANNNNNTGG 1 cut(s) 580
AciI CCGC 3 cut(s) 6, 195, 509
AclWI GGATC 4 cut(s) 452, 520, 1097, 1110
AcoI YGGCCR 2 cut(s) 3, 1076
AcuI CTGAAG 2 cut(s) 702, 1092
AfaI GTAC 1 cut(s) 336
AfiI CCNNNNNNNGG 2 cut(s) 580, 1112
AgsI TTSAA 3 cut(s) 448, 529, 561
AjnI CCWGG 4 cut(s) 881, 1030, 1093, 1129
AjuI GAANNNNNNNTTGG 2 cut(s) 582, 614
Alw21I GWGCWC 2 cut(s) 796, 997
Alw26I GTCTC 1 cut(s) 285
AlwI GGATC 4 cut(s) 452, 520, 1097, 1110
AoxI GGCC 5 cut(s) 3, 473, 769, 879, 1076
ApeKI GCWGC 4 cut(s) 138, 141, 673, 782
ArsI GACNNNNNNTTYG 2 cut(s) 302, 334
AspLEI GCGC 1 cut(s) 979
AspS9I GGNCC 1 cut(s) 348
AsuHPI GGTGA 2 cut(s) 85, 1082
AvaII GGWCC 1 cut(s) 348
BalI TGGCCA 1 cut(s) 1078
BamHI GGATCC 1 cut(s) 1102
BanI GGYRCC 1 cut(s) 804
BanII GRGCYC 1 cut(s) 796
BarI GAAGNNNNNNTAC 2 cut(s) 1031, 1063
BauI CACGAG 2 cut(s) 129, 165
BbsI GAAGAC 1 cut(s) 163
Bbv12I GWGCWC 2 cut(s) 796, 997
BbvI GCAGC 4 cut(s) 125, 128, 660, 769
BccI CCATC 4 cut(s) 133, 224, 816, 893
BceAI ACGGC 1 cut(s) 129
BcgI CGANNNNNNTGC 2 cut(s) 297, 331
BciT130I CCWGG 4 cut(s) 883, 1032, 1095, 1131
BcoDI GTCTC 1 cut(s) 285
BfaI CTAG 2 cut(s) 434, 744
BfmI CTRYAG 3 cut(s) 696, 852, 941
BglII AGATCT 1 cut(s) 1136
BisI GCNGC 5 cut(s) 6, 139, 142, 674, 783
BlsI GCNGC 5 cut(s) 7, 140, 143, 675, 784
Bme1390I CCNGG 4 cut(s) 883, 1032, 1095, 1131
Bme18I GGWCC 1 cut(s) 348
BmgT120I GGNCC 1 cut(s) 348
BmiI GGNNCC 3 cut(s) 349, 806, 1104
BmrFI CCNGG 4 cut(s) 883, 1032, 1095, 1131
BmsI GCATC 6 cut(s) 295, 838, 943, 1052, 1111, 1133
BpiI GAAGAC 1 cut(s) 163
BpmI CTGGAG 3 cut(s) 271, 1113, 1134
Bpu10I CCTNAGC 1 cut(s) 263
BpuEI CTTGAG 4 cut(s) 58, 194, 570, 1009
BsaBI GATNNNNATC 2 cut(s) 426, 957
BsaJI CCNNGG 2 cut(s) 1066, 1094
Bsc4I CCNNNNNNNGG 2 cut(s) 580, 1112
Bse1I ACTGG 1 cut(s) 1079
Bse3DI GCAATG 1 cut(s) 753
Bse8I GATNNNNATC 2 cut(s) 426, 957
BseBI CCWGG 4 cut(s) 883, 1032, 1095, 1131
BseDI CCNNGG 2 cut(s) 1066, 1094
BseGI GGATG 3 cut(s) 310, 958, 1067
BseJI GATNNNNATC 2 cut(s) 426, 957
BseLI CCNNNNNNNGG 2 cut(s) 580, 1112
BseMI GCAATG 1 cut(s) 753
BseMII CTCAG 2 cut(s) 231, 277
BseNI ACTGG 1 cut(s) 1079
BseRI GAGGAG 1 cut(s) 166
BseXI GCAGC 4 cut(s) 125, 128, 660, 769
BshFI GGCC 5 cut(s) 5, 475, 771, 881, 1078
BshNI GGYRCC 1 cut(s) 804
BsiHKAI GWGCWC 2 cut(s) 796, 997
BslFI GGGAC 1 cut(s) 282
BslI CCNNNNNNNGG 2 cut(s) 580, 1112
BsmAI GTCTC 1 cut(s) 285
BsmFI GGGAC 1 cut(s) 282
BsnI GGCC 5 cut(s) 5, 475, 771, 881, 1078
Bsp1286I GDGCHC 2 cut(s) 796, 997
Bsp143I GATC 6 cut(s) 427, 457, 512, 958, 1102, 1136
BspACI CCGC 3 cut(s) 6, 195, 509
BspANI GGCC 5 cut(s) 5, 475, 771, 881, 1078
BspCNI CTCAG 2 cut(s) 232, 276
BspLI GGNNCC 3 cut(s) 349, 806, 1104
BspPI GGATC 4 cut(s) 452, 520, 1097, 1110
BspQI GCTCTTC 2 cut(s) 784, 1034
BspT107I GGYRCC 1 cut(s) 804
BsrDI GCAATG 1 cut(s) 753
BsrI ACTGG 1 cut(s) 1079
BssECI CCNNGG 2 cut(s) 1066, 1094
BssMI GATC 6 cut(s) 427, 457, 512, 958, 1102, 1136
BssSI CACGAG 2 cut(s) 129, 165
Bst2BI CACGAG 2 cut(s) 129, 165
Bst2UI CCWGG 4 cut(s) 883, 1032, 1095, 1131
Bst4CI ACNGT 3 cut(s) 381, 973, 1005
Bst6I CTCTTC 4 cut(s) 61, 784, 870, 1034
BstAPI GCANNNNNTGC 2 cut(s) 713, 755
BstC8I GCNNGC 5 cut(s) 399, 678, 773, 981, 985
BstDEI CTNAG 2 cut(s) 240, 263
BstF5I GGATG 3 cut(s) 310, 958, 1067
BstHHI GCGC 1 cut(s) 979
BstKTI GATC 6 cut(s) 430, 460, 515, 961, 1105, 1139
BstMAI GTCTC 1 cut(s) 285
BstMBI GATC 6 cut(s) 427, 457, 512, 958, 1102, 1136
BstMWI GCNNNNNNNGC 4 cut(s) 713, 755, 791, 1062
BstNI CCWGG 4 cut(s) 883, 1032, 1095, 1131
BstNSI RCATGY 1 cut(s) 987
BstSCI CCNGG 4 cut(s) 881, 1030, 1093, 1129
BstSFI CTRYAG 3 cut(s) 696, 852, 941
BstV1I GCAGC 4 cut(s) 125, 128, 660, 769
BstV2I GAAGAC 1 cut(s) 163
BstX2I RGATCY 3 cut(s) 512, 1102, 1136
BstXI CCANNNNNNTGG 1 cut(s) 902
BstYI RGATCY 3 cut(s) 512, 1102, 1136
BsuRI GGCC 5 cut(s) 5, 475, 771, 881, 1078
BtsCI GGATG 3 cut(s) 310, 958, 1067
BtsI GCAGTG 1 cut(s) 24
BtsIMutI CAGTG 1 cut(s) 24
Cac8I GCNNGC 5 cut(s) 399, 678, 773, 981, 985
CfoI GCGC 1 cut(s) 979
Cfr13I GGNCC 1 cut(s) 348
Csp6I GTAC 1 cut(s) 335
CviAII CATG 5 cut(s) 455, 492, 669, 740, 984
CviQI GTAC 1 cut(s) 335
DdeI CTNAG 2 cut(s) 240, 263
DpnI GATC 6 cut(s) 429, 459, 514, 960, 1104, 1138
DpnII GATC 6 cut(s) 427, 457, 512, 958, 1102, 1136
DraI TTTAAA 2 cut(s) 150, 468
DrdI GACNNNNNNGTC 1 cut(s) 932
DseDI GACNNNNNNGTC 1 cut(s) 932
EaeI YGGCCR 2 cut(s) 3, 1076
Eam1104I CTCTTC 4 cut(s) 61, 784, 870, 1034
EarI CTCTTC 4 cut(s) 61, 784, 870, 1034
Ecl136II GAGCTC 1 cut(s) 794
Eco24I GRGCYC 1 cut(s) 796
Eco47I GGWCC 1 cut(s) 348
Eco53kI GAGCTC 1 cut(s) 794
Eco57I CTGAAG 2 cut(s) 702, 1092
EcoICRI GAGCTC 1 cut(s) 794
EcoO109I RGGNCCY 1 cut(s) 348
EcoRII CCWGG 4 cut(s) 881, 1030, 1093, 1129
EcoT22I ATGCAT 1 cut(s) 1126
EcoT38I GRGCYC 1 cut(s) 796
FaeI CATG 5 cut(s) 458, 495, 672, 743, 987
FaqI GGGAC 1 cut(s) 282
FatI CATG 5 cut(s) 454, 491, 668, 739, 983
FauI CCCGC 1 cut(s) 502
FauNDI CATATG 1 cut(s) 646
Fnu4HI GCNGC 5 cut(s) 6, 139, 142, 674, 783
FokI GGATG 3 cut(s) 317, 965, 1074
FriOI GRGCYC 1 cut(s) 796
Fsp4HI GCNGC 5 cut(s) 6, 139, 142, 674, 783
FspBI CTAG 2 cut(s) 434, 744
FspI TGCGCA 1 cut(s) 978
GlaI GCGC 1 cut(s) 978
GluI GCNGC 5 cut(s) 6, 139, 142, 674, 783
GsuI CTGGAG 3 cut(s) 271, 1113, 1134
HaeIII GGCC 5 cut(s) 5, 475, 771, 881, 1078
HhaI GCGC 1 cut(s) 979
Hin1II CATG 5 cut(s) 458, 495, 672, 743, 987
Hin6I GCGC 1 cut(s) 977
HinP1I GCGC 1 cut(s) 977
HincII GTYRAC 1 cut(s) 664
HindII GTYRAC 1 cut(s) 664
HindIII AAGCTT 2 cut(s) 184, 1025
HinfI GANTC 6 cut(s) 488, 497, 557, 665, 692, 886
HphI GGTGA 2 cut(s) 85, 1082
Hpy166II GTNNAC 1 cut(s) 664
Hpy188I TCNGA 2 cut(s) 691, 1018
Hpy188III TCNNGA 5 cut(s) 75, 227, 288, 461, 587
Hpy8I GTNNAC 1 cut(s) 664
HpyAV CCTTC 3 cut(s) 677, 854, 1116
HpyCH4III ACNGT 3 cut(s) 381, 973, 1005
HpyCH4IV ACGT 2 cut(s) 927, 963
HpyCH4V TGCA 8 cut(s) 49, 308, 520, 596, 869, 923, 956, 1124
HpyF10VI GCNNNNNNNGC 4 cut(s) 713, 755, 791, 1062
HpyF3I CTNAG 2 cut(s) 240, 263
HpySE526I ACGT 2 cut(s) 927, 963
Hsp92II CATG 5 cut(s) 458, 495, 672, 743, 987
HspAI GCGC 1 cut(s) 977
Kzo9I GATC 6 cut(s) 427, 457, 512, 958, 1102, 1136
LguI GCTCTTC 2 cut(s) 784, 1034
LmnI GCTCC 2 cut(s) 290, 779
Lsp1109I GCAGC 4 cut(s) 125, 128, 660, 769
LweI GCATC 6 cut(s) 295, 838, 943, 1052, 1111, 1133
MaeI CTAG 2 cut(s) 434, 744
MaeII ACGT 2 cut(s) 927, 963
MaeIII GTNAC 4 cut(s) 202, 355, 837, 1070
MalI GATC 6 cut(s) 429, 459, 514, 960, 1104, 1138
MboI GATC 6 cut(s) 427, 457, 512, 958, 1102, 1136
MboII GAAGA 7 cut(s) 48, 163, 437, 460, 801, 887, 1051
MflI RGATCY 3 cut(s) 512, 1102, 1136
MhlI GDGCHC 2 cut(s) 796, 997
MlsI TGGCCA 1 cut(s) 1078
MluCI AATT 5 cut(s) 40, 97, 248, 540, 567
MluNI TGGCCA 1 cut(s) 1078
MlyI GAGTC 4 cut(s) 497, 506, 659, 895
Mox20I TGGCCA 1 cut(s) 1078
Mph1103I ATGCAT 1 cut(s) 1126
MscI TGGCCA 1 cut(s) 1078
MseI TTAA 9 cut(s) 32, 149, 323, 467, 503, 570, 731, 966, 999
MslI CAYNNNNRTG 2 cut(s) 299, 362
Msp20I TGGCCA 1 cut(s) 1078
MspA1I CMGCKG 1 cut(s) 1112
MspR9I CCNGG 4 cut(s) 883, 1032, 1095, 1131
MvaI CCWGG 4 cut(s) 883, 1032, 1095, 1131
MwoI GCNNNNNNNGC 4 cut(s) 713, 755, 791, 1062
NdeI CATATG 1 cut(s) 646
NdeII GATC 6 cut(s) 427, 457, 512, 958, 1102, 1136
NlaIII CATG 5 cut(s) 458, 495, 672, 743, 987
NlaIV GGNNCC 3 cut(s) 349, 806, 1104
NmeAIII GCCGAG 1 cut(s) 1091
NmuCI GTSAC 3 cut(s) 355, 837, 1070
NsbI TGCGCA 1 cut(s) 978
NsiI ATGCAT 1 cut(s) 1126
NspI RCATGY 1 cut(s) 987
PaeI GCATGC 1 cut(s) 987
PciSI GCTCTTC 2 cut(s) 784, 1034
PfeI GAWTC 2 cut(s) 557, 692
PflFI GACNNNGTC 1 cut(s) 482
PflMI CCANNNNNTGG 1 cut(s) 580
PkrI GCNGC 5 cut(s) 7, 140, 143, 675, 784
PleI GAGTC 4 cut(s) 496, 505, 659, 894
PpsI GAGTC 4 cut(s) 496, 505, 659, 894
PpuMI RGGWCCY 1 cut(s) 348
Psp124BI GAGCTC 1 cut(s) 796
Psp5II RGGWCCY 1 cut(s) 348
Psp6I CCWGG 4 cut(s) 881, 1030, 1093, 1129
PspGI CCWGG 4 cut(s) 881, 1030, 1093, 1129
PspN4I GGNNCC 3 cut(s) 349, 806, 1104
PspPI GGNCC 1 cut(s) 348
PspPPI RGGWCCY 1 cut(s) 348
PsuI RGATCY 3 cut(s) 512, 1102, 1136
PsyI GACNNNGTC 1 cut(s) 482
PvuII CAGCTG 1 cut(s) 1112
RsaI GTAC 1 cut(s) 336
RsaNI GTAC 1 cut(s) 335
RseI CAYNNNNRTG 2 cut(s) 299, 362
SacI GAGCTC 1 cut(s) 796
SapI GCTCTTC 2 cut(s) 784, 1034
SaqAI TTAA 9 cut(s) 32, 149, 323, 467, 503, 570, 731, 966, 999
SatI GCNGC 5 cut(s) 6, 139, 142, 674, 783
Sau3AI GATC 6 cut(s) 427, 457, 512, 958, 1102, 1136
Sau96I GGNCC 1 cut(s) 348
SchI GAGTC 4 cut(s) 497, 506, 659, 895
ScrFI CCNGG 4 cut(s) 883, 1032, 1095, 1131
SduI GDGCHC 2 cut(s) 796, 997
SfaNI GCATC 6 cut(s) 295, 838, 943, 1052, 1111, 1133
SfcI CTRYAG 3 cut(s) 696, 852, 941
SinI GGWCC 1 cut(s) 348
SmiMI CAYNNNNRTG 2 cut(s) 299, 362
SmlI CTYRAG 4 cut(s) 73, 209, 585, 988
SmoI CTYRAG 4 cut(s) 73, 209, 585, 988
SphI GCATGC 1 cut(s) 987
Sse9I AATT 5 cut(s) 40, 97, 248, 540, 567
SsiI CCGC 3 cut(s) 6, 195, 509
SspMI CTAG 2 cut(s) 434, 744
SstI GAGCTC 1 cut(s) 796
StyD4I CCNGG 4 cut(s) 881, 1030, 1093, 1129
TaaI ACNGT 3 cut(s) 381, 973, 1005
TaiI ACGT 2 cut(s) 930, 966
TaqI TCGA 1 cut(s) 486
TasI AATT 5 cut(s) 40, 97, 248, 540, 567
TatI WGTACW 1 cut(s) 334
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 2 cut(s) 557, 692
Tru1I TTAA 9 cut(s) 32, 149, 323, 467, 503, 570, 731, 966, 999
Tru9I TTAA 9 cut(s) 32, 149, 323, 467, 503, 570, 731, 966, 999
TscAI CASTG 1 cut(s) 31
TseFI GTSAC 3 cut(s) 355, 837, 1070
TseI GCWGC 4 cut(s) 138, 141, 673, 782
Tsp45I GTSAC 3 cut(s) 355, 837, 1070
TspDTI ATGAA 2 cut(s) 438, 873
TspGWI ACGGA 1 cut(s) 792
TspRI CASTG 1 cut(s) 31
Tth111I GACNNNGTC 1 cut(s) 482
Van91I CCANNNNNTGG 1 cut(s) 580
VpaK11BI GGWCC 1 cut(s) 348
XceI RCATGY 1 cut(s) 987
XcmI CCANNNNNNNNNTGG 1 cut(s) 468
XspI CTAG 2 cut(s) 434, 744
Zsp2I ATGCAT 1 cut(s) 1126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.