AT2G40300

Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0008237
Physical Location & Seq
Reverse (-)
16831109 .. 16833294
2186 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G40300.1

Sequence Viewer

Length: 780 bp
ATGCTTCTCAAGACTGTTTCTTCATCTTCTTCTTCAGCTCTCTCGCTGGTGAATTTCCATGGCGTGAAGAAGGATGTGTCTCCTTTGTTACCTTCCATTTCATCGAATCTTCGAGTTTCTTCTGGAAAATCTGGAAACTTAACTTTCTCTTTTCGCGCATCTAAAAGCTCAACCACCGACGCGCTAAGCGGCGTTGTCTTCGAGCCGTTTAAGGAAGTAAAAAAGGAGCTCGATCTCGTCCCTACCAGCTCTCATCTCTCACTCGCTCGACAAAAGTACTCAGACGAGTGCGAAGCCGCCATTAACGAGCAGATCAATGTGGAATACAATGTCTCGTATGTGTATCACGCTATGTATGCTTACTTTGATCGGGATAACATCGCGCTCAAAGGTCTTGCCAAGTTCTTTAAGGAATCAAGTTTGGAAGAAAGAGAGCATGCTGAGAAGTTAATGGAGTATCAGAACAAACGTGGTGGGAGGGTTAAGTTACAGTCCATTGTAATGCCTCTTTCAGAGTTTGAACATGTTGACAAAGGAGATGCTCTTTATGGCATGGAGCTTGCTCTGTCACTGGAGAAACTAGTTAATGAGAAGCTCTTAAACCTTCACAGTGTTGCTTCGAAGAACAATGATGTCCACTTGGCAGATTTTATTGAGAGCGAGTTTCTGACAGAGCAGGTGGAAGCAATCAAGTTGATCTCAGAATATGTGGCTCAACTGCGACGAGTTGGCAAAGGACACGGAACATGGCATTTCAATCAGATGCTTCTGGAAGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000041 GO:0000302 GO:0001101 GO:0003006 GO:0003674 GO:0005488 GO:0005506 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006810 GO:0006811 GO:0006812 GO:0006826 GO:0006873 GO:0006875 GO:0006879 GO:0006880 GO:0006950 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009507 GO:0009526 GO:0009532 GO:0009534 GO:0009535 GO:0009536 GO:0009570 GO:0009579 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009636 GO:0009719 GO:0009725 GO:0009735 GO:0009737 GO:0009791 GO:0009908 GO:0009941 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010043 GO:0015979 GO:0016020 GO:0019725 GO:0019904 GO:0022414 GO:0030001 GO:0030003 GO:0031967 GO:0031975 GO:0031976 GO:0031984 GO:0032501 GO:0032502 GO:0033993 GO:0034357 GO:0042221 GO:0042493 GO:0042542 GO:0042592 GO:0042651 GO:0043167 GO:0043169 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0046677 GO:0046872 GO:0046914 GO:0046916 GO:0048366 GO:0048367 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0048878 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051235 GO:0051238 GO:0051641 GO:0051651 GO:0051704 GO:0051707 GO:0055035 GO:0055065 GO:0055072 GO:0055076 GO:0055080 GO:0055082 GO:0061458 GO:0065007 GO:0065008 GO:0090567 GO:0097305 GO:0097577 GO:0098771 GO:0099402 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko04216 ko04217 ko04978 map04216 map04217 map04978
Pfam Domains
Protein Families
Ferritin

Protein Analysis

259

Amino Acids

29.03

Weight (kDa)

6.16

Isoelectric Point (pI)

37.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ferritin PF00210 99 - 243 2.1e-33 Ferritin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 667
Acc36I ACCTGC 1 cut(s) 667
AccII CGCG 3 cut(s) 156, 182, 383
AciI CCGC 2 cut(s) 189, 297
AcsI RAATTY 1 cut(s) 52
AcuI CTGAAG 1 cut(s) 18
AfaI GTAC 1 cut(s) 278
AflIII ACRYGT 1 cut(s) 523
AgsI TTSAA 2 cut(s) 521, 757
AhlI ACTAGT 1 cut(s) 580
AluBI AGCT 6 cut(s) 38, 168, 229, 249, 559, 595
AluI AGCT 6 cut(s) 38, 168, 229, 249, 559, 595
Alw21I GWGCWC 1 cut(s) 231
Alw26I GTCTC 2 cut(s) 84, 337
ApoI RAATTY 1 cut(s) 52
AspLEI GCGC 3 cut(s) 158, 184, 385
AsuHPI GGTGA 1 cut(s) 61
AsuII TTCGAA 1 cut(s) 620
BanII GRGCYC 1 cut(s) 231
BbsI GAAGAC 1 cut(s) 190
Bbv12I GWGCWC 1 cut(s) 231
BceAI ACGGC 1 cut(s) 190
BcoDI GTCTC 2 cut(s) 84, 337
BcuI ACTAGT 1 cut(s) 580
BfaI CTAG 1 cut(s) 581
BfuAI ACCTGC 1 cut(s) 667
BisI GCNGC 2 cut(s) 190, 297
BlpI GCTNAGC 1 cut(s) 185
BlsI GCNGC 2 cut(s) 191, 298
BmcAI AGTACT 1 cut(s) 278
BmsI GCATC 3 cut(s) 167, 529, 753
BpiI GAAGAC 1 cut(s) 190
BpmI CTGGAG 1 cut(s) 593
Bpu1102I GCTNAGC 1 cut(s) 185
Bpu14I TTCGAA 1 cut(s) 620
BsaJI CCNNGG 1 cut(s) 58
Bse1I ACTGG 1 cut(s) 576
BseDI CCNNGG 1 cut(s) 58
BseGI GGATG 1 cut(s) 79
BseMII CTCAG 3 cut(s) 294, 432, 714
BseNI ACTGG 1 cut(s) 576
Bsh1236I CGCG 3 cut(s) 156, 182, 383
BsiHKAI GWGCWC 1 cut(s) 231
BslFI GGGAC 1 cut(s) 224
BsmAI GTCTC 2 cut(s) 84, 337
BsmFI GGGAC 1 cut(s) 224
Bsp119I TTCGAA 1 cut(s) 620
Bsp1286I GDGCHC 1 cut(s) 231
Bsp143I GATC 4 cut(s) 232, 312, 367, 696
Bsp1720I GCTNAGC 1 cut(s) 185
Bsp19I CCATGG 1 cut(s) 58
BspACI CCGC 2 cut(s) 189, 297
BspCNI CTCAG 3 cut(s) 293, 433, 713
BspFNI CGCG 3 cut(s) 156, 182, 383
BspMI ACCTGC 1 cut(s) 667
BspT104I TTCGAA 1 cut(s) 620
BsrI ACTGG 1 cut(s) 576
BssECI CCNNGG 1 cut(s) 58
BssMI GATC 4 cut(s) 232, 312, 367, 696
BssT1I CCWWGG 1 cut(s) 58
Bst4CI ACNGT 3 cut(s) 16, 492, 611
BstBI TTCGAA 1 cut(s) 620
BstC8I GCNNGC 2 cut(s) 438, 561
BstDEI CTNAG 4 cut(s) 185, 280, 441, 700
BstDSI CCRYGG 1 cut(s) 58
BstF5I GGATG 1 cut(s) 79
BstFNI CGCG 3 cut(s) 156, 182, 383
BstHHI GCGC 3 cut(s) 158, 184, 385
BstKTI GATC 4 cut(s) 235, 315, 370, 699
BstMAI GTCTC 2 cut(s) 84, 337
BstMBI GATC 4 cut(s) 232, 312, 367, 696
BstMWI GCNNNNNNNGC 1 cut(s) 356
BstNSI RCATGY 2 cut(s) 440, 527
BstUI CGCG 3 cut(s) 156, 182, 383
BstV2I GAAGAC 1 cut(s) 190
BtgI CCRYGG 1 cut(s) 58
BtgZI GCGATG 1 cut(s) 364
BtsCI GGATG 1 cut(s) 79
BtsIMutI CAGTG 2 cut(s) 569, 616
BveI ACCTGC 1 cut(s) 667
Cac8I GCNNGC 2 cut(s) 438, 561
CfoI GCGC 3 cut(s) 158, 184, 385
CseI GACGC 1 cut(s) 188
Csp6I GTAC 1 cut(s) 277
CspCI CAANNNNNGTGG 2 cut(s) 454, 489
CviAII CATG 5 cut(s) 59, 437, 524, 553, 747
CviJI RGCY 9 cut(s) 38, 168, 205, 229, 249, 296, 559, 595, 713
CviKI_1 RGCY 9 cut(s) 38, 168, 205, 229, 249, 296, 559, 595, 713
CviQI GTAC 1 cut(s) 277
DdeI CTNAG 4 cut(s) 185, 280, 441, 700
DpnI GATC 4 cut(s) 234, 314, 369, 698
DpnII GATC 4 cut(s) 232, 312, 367, 696
Ecl136II GAGCTC 1 cut(s) 229
Eco130I CCWWGG 1 cut(s) 58
Eco24I GRGCYC 1 cut(s) 231
Eco53kI GAGCTC 1 cut(s) 229
Eco57I CTGAAG 1 cut(s) 18
EcoICRI GAGCTC 1 cut(s) 229
EcoT14I CCWWGG 1 cut(s) 58
EcoT38I GRGCYC 1 cut(s) 231
ErhI CCWWGG 1 cut(s) 58
FaeI CATG 5 cut(s) 62, 440, 527, 556, 750
FaqI GGGAC 1 cut(s) 224
FatI CATG 5 cut(s) 58, 436, 523, 552, 746
Fnu4HI GCNGC 2 cut(s) 190, 297
FokI GGATG 1 cut(s) 86
FriOI GRGCYC 1 cut(s) 231
Fsp4HI GCNGC 2 cut(s) 190, 297
FspBI CTAG 1 cut(s) 581
GlaI GCGC 3 cut(s) 157, 183, 384
GluI GCNGC 2 cut(s) 190, 297
GsuI CTGGAG 1 cut(s) 593
HgaI GACGC 1 cut(s) 188
HhaI GCGC 3 cut(s) 158, 184, 385
Hin1II CATG 5 cut(s) 62, 440, 527, 556, 750
Hin6I GCGC 3 cut(s) 156, 182, 383
HinP1I GCGC 3 cut(s) 156, 182, 383
HincII GTYRAC 1 cut(s) 529
HindII GTYRAC 1 cut(s) 529
HinfI GANTC 2 cut(s) 106, 413
HphI GGTGA 1 cut(s) 61
Hpy166II GTNNAC 2 cut(s) 529, 637
Hpy188I TCNGA 6 cut(s) 283, 462, 514, 669, 703, 762
Hpy188III TCNNGA 5 cut(s) 10, 123, 132, 371, 770
Hpy8I GTNNAC 2 cut(s) 529, 637
Hpy99I CGWCG 2 cut(s) 182, 726
HpyAV CCTTC 4 cut(s) 64, 102, 614, 767
HpyCH4III ACNGT 3 cut(s) 16, 492, 611
HpyCH4IV ACGT 1 cut(s) 469
HpyF10VI GCNNNNNNNGC 1 cut(s) 356
HpyF3I CTNAG 4 cut(s) 185, 280, 441, 700
HpySE526I ACGT 1 cut(s) 469
Hsp92II CATG 5 cut(s) 62, 440, 527, 556, 750
HspAI GCGC 3 cut(s) 156, 182, 383
Kzo9I GATC 4 cut(s) 232, 312, 367, 696
LmnI GCTCC 2 cut(s) 226, 556
LpnPI CCDG 7 cut(s) 32, 108, 117, 259, 557, 662, 755
LweI GCATC 3 cut(s) 167, 529, 753
MaeI CTAG 1 cut(s) 581
MaeII ACGT 1 cut(s) 469
MaeIII GTNAC 3 cut(s) 87, 486, 567
MalI GATC 4 cut(s) 234, 314, 369, 698
MboI GATC 4 cut(s) 232, 312, 367, 696
MhlI GDGCHC 1 cut(s) 231
MluCI AATT 1 cut(s) 52
MnlI CCTC 2 cut(s) 471, 516
MseI TTAA 8 cut(s) 140, 210, 303, 408, 449, 483, 585, 599
MslI CAYNNNNRTG 1 cut(s) 500
MvnI CGCG 3 cut(s) 156, 182, 383
MwoI GCNNNNNNNGC 1 cut(s) 356
NcoI CCATGG 1 cut(s) 58
NdeII GATC 4 cut(s) 232, 312, 367, 696
NlaIII CATG 5 cut(s) 62, 440, 527, 556, 750
NmuCI GTSAC 1 cut(s) 567
NspI RCATGY 2 cut(s) 440, 527
NspV TTCGAA 1 cut(s) 620
PaeI GCATGC 1 cut(s) 440
PaqCI CACCTGC 1 cut(s) 667
PciI ACATGT 1 cut(s) 523
PfeI GAWTC 2 cut(s) 106, 413
PkrI GCNGC 2 cut(s) 191, 298
PscI ACATGT 1 cut(s) 523
Psp124BI GAGCTC 1 cut(s) 231
RsaI GTAC 1 cut(s) 278
RsaNI GTAC 1 cut(s) 277
RseI CAYNNNNRTG 1 cut(s) 500
SacI GAGCTC 1 cut(s) 231
SaqAI TTAA 8 cut(s) 140, 210, 303, 408, 449, 483, 585, 599
SatI GCNGC 2 cut(s) 190, 297
Sau3AI GATC 4 cut(s) 232, 312, 367, 696
ScaI AGTACT 1 cut(s) 278
SduI GDGCHC 1 cut(s) 231
SfaNI GCATC 3 cut(s) 167, 529, 753
SfuI TTCGAA 1 cut(s) 620
SmiMI CAYNNNNRTG 1 cut(s) 500
SmlI CTYRAG 1 cut(s) 8
SmoI CTYRAG 1 cut(s) 8
SpeI ACTAGT 1 cut(s) 580
SphI GCATGC 1 cut(s) 440
Sse9I AATT 1 cut(s) 52
SsiI CCGC 2 cut(s) 189, 297
SspMI CTAG 1 cut(s) 581
SstI GAGCTC 1 cut(s) 231
StyI CCWWGG 1 cut(s) 58
TaaI ACNGT 3 cut(s) 16, 492, 611
TaiI ACGT 1 cut(s) 472
TaqI TCGA 6 cut(s) 104, 112, 201, 231, 268, 620
TasI AATT 1 cut(s) 52
TatI WGTACW 1 cut(s) 276
TauI GCSGC 2 cut(s) 192, 299
TfiI GAWTC 2 cut(s) 106, 413
Tru1I TTAA 8 cut(s) 140, 210, 303, 408, 449, 483, 585, 599
Tru9I TTAA 8 cut(s) 140, 210, 303, 408, 449, 483, 585, 599
TscAI CASTG 2 cut(s) 576, 616
TseFI GTSAC 1 cut(s) 567
Tsp45I GTSAC 1 cut(s) 567
TspDTI ATGAA 2 cut(s) 12, 90
TspGWI ACGGA 1 cut(s) 756
TspRI CASTG 2 cut(s) 576, 616
XapI RAATTY 1 cut(s) 52
XceI RCATGY 2 cut(s) 440, 527
XspI CTAG 1 cut(s) 581
ZrmI AGTACT 1 cut(s) 278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.