AT2G44050

Catalyzes the formation of 6,7-dimethyl-8- ribityllumazine by condensation of 5-amino-6-(D- ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0008755
Physical Location & Seq
Forward (+)
18224213 .. 18226233
2021 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G44050.1

Sequence Viewer

Length: 684 bp
ATGAAGTCATTAGCTTCGCCGCCGTGTCTCCGCCTGATACCGACGGCACACCGTCAGCTCAATTCGCGTCAATCTTCCTCCGCCTGTTATATACACGGTGGCTCTTCTGTGAACAAATCCAATAATCTCTCATTCTCCTCATCCACATCCGGATTTGCGTCACCACTAGCTGTAGAGAAGGAATTACGCTCTTCATTCGTACAGACGGCTGCTGTTCGCCATGTTACGGGGTCTCTTATCAGAGGCGAAGGTCTTAGATTCGCCATCGTGGTAGCTCGTTTCAATGAGGTTGTGACTAAGTTGCTTTTGGAAGGAGCGATTGAGACTTTCAAGAAGTATTCAGTCAGAGAAGAAGACATTGAAGTTATTTGGGTTCCTGGCAGCTTTGAAATTGGTGTTGTTGCACAAAATCTTGGGAAATCGGGAAAATTTCATGCTGTTTTATGTATCGGCGCTGTGATAAGAGGAGATACCACACATTATGATGCTGTTGCCAACTCTGCTGCGTCTGGAGTACTTTCTGCTAGCATAAATTCAGGCGTTCCATGCATATTTGGTGTACTGACTTGCGAGGACATGGATCAGGCTCTGAATCGATCTGGTGGCAAAGCCGGCAATAAGGGAGCTGAAACTGCTTTGACGGCGCTCGAAATGGCGTCGTTGTTTGAGCACCACCTGAAATAG

Protein Analysis

227

Amino Acids

24.02

Weight (kDa)

8.62

Isoelectric Point (pI)

41.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DMRL_synthase PF00885 84 - 222 3.2e-50 6,7-dimethyl-8-ribityllumazine synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 67
AccIII TCCGGA 1 cut(s) 149
AciI CCGC 3 cut(s) 20, 31, 81
AclWI GGATC 1 cut(s) 588
AcsI RAATTY 2 cut(s) 428, 532
AcyI GRCGYC 1 cut(s) 656
AfaI GTAC 3 cut(s) 201, 516, 561
AfiI CCNNNNNNNGG 1 cut(s) 226
AgsI TTSAA 4 cut(s) 283, 331, 362, 389
AjnI CCWGG 1 cut(s) 376
AluBI AGCT 6 cut(s) 14, 58, 170, 275, 384, 626
AluI AGCT 6 cut(s) 14, 58, 170, 275, 384, 626
Alw21I GWGCWC 1 cut(s) 672
Alw26I GTCTC 3 cut(s) 32, 237, 317
AlwI GGATC 1 cut(s) 588
AlwNI CAGNNNCTG 1 cut(s) 589
Aor13HI TCCGGA 1 cut(s) 149
ApeKI GCWGC 3 cut(s) 209, 381, 503
ApoI RAATTY 2 cut(s) 428, 532
AspLEI GCGC 2 cut(s) 455, 646
AsuHPI GGTGA 1 cut(s) 153
AsuNHI GCTAGC 1 cut(s) 524
BbsI GAAGAC 1 cut(s) 360
Bbv12I GWGCWC 1 cut(s) 672
BbvI GCAGC 3 cut(s) 196, 393, 490
BccI CCATC 1 cut(s) 272
BceAI ACGGC 4 cut(s) 7, 60, 222, 657
BciT130I CCWGG 1 cut(s) 378
BcoDI GTCTC 3 cut(s) 32, 237, 317
BfaI CTAG 2 cut(s) 167, 525
BfmI CTRYAG 1 cut(s) 171
BfoI RGCGCY 2 cut(s) 456, 647
BisI GCNGC 4 cut(s) 20, 210, 382, 504
BlsI GCNGC 4 cut(s) 21, 211, 383, 505
BmcAI AGTACT 1 cut(s) 516
Bme1390I CCNGG 1 cut(s) 378
BmiI GGNNCC 1 cut(s) 375
BmrFI CCNGG 1 cut(s) 378
BmsI GCATC 1 cut(s) 475
BmtI GCTAGC 1 cut(s) 528
BpiI GAAGAC 1 cut(s) 360
BpmI CTGGAG 1 cut(s) 531
Bsa29I ATCGAT 1 cut(s) 595
BsaHI GRCGYC 1 cut(s) 656
BsaI GGTCTC 1 cut(s) 237
BsaWI WCCGGW 1 cut(s) 149
Bsc4I CCNNNNNNNGG 1 cut(s) 226
Bse118I RCCGGY 1 cut(s) 611
BseAI TCCGGA 1 cut(s) 149
BseBI CCWGG 1 cut(s) 378
BseCI ATCGAT 1 cut(s) 595
BseGI GGATG 2 cut(s) 140, 146
BseLI CCNNNNNNNGG 1 cut(s) 226
BseRI GAGGAG 2 cut(s) 127, 480
BseXI GCAGC 3 cut(s) 196, 393, 490
Bsh1236I CGCG 1 cut(s) 67
BshVI ATCGAT 1 cut(s) 595
BsiHKAI GWGCWC 1 cut(s) 672
BsiSI CCGG 2 cut(s) 150, 612
BslI CCNNNNNNNGG 1 cut(s) 226
BsmAI GTCTC 3 cut(s) 32, 237, 317
Bso31I GGTCTC 1 cut(s) 237
Bsp1286I GDGCHC 1 cut(s) 672
Bsp13I TCCGGA 1 cut(s) 149
Bsp143I GATC 2 cut(s) 580, 596
BspACI CCGC 3 cut(s) 20, 31, 81
BspDI ATCGAT 1 cut(s) 595
BspEI TCCGGA 1 cut(s) 149
BspFNI CGCG 1 cut(s) 67
BspLI GGNNCC 1 cut(s) 375
BspOI GCTAGC 1 cut(s) 528
BspPI GGATC 1 cut(s) 588
BspQI GCTCTTC 2 cut(s) 109, 196
BspTNI GGTCTC 1 cut(s) 237
BsrFI RCCGGY 1 cut(s) 611
BssAI RCCGGY 1 cut(s) 611
BssMI GATC 2 cut(s) 580, 596
BssNI GRCGYC 1 cut(s) 656
Bst2UI CCWGG 1 cut(s) 378
Bst4CI ACNGT 2 cut(s) 53, 98
Bst6I CTCTTC 2 cut(s) 109, 196
BstACI GRCGYC 1 cut(s) 656
BstC8I GCNNGC 2 cut(s) 526, 613
BstDEI CTNAG 2 cut(s) 254, 297
BstF5I GGATG 2 cut(s) 140, 146
BstFNI CGCG 1 cut(s) 67
BstH2I RGCGCY 2 cut(s) 456, 647
BstHHI GCGC 2 cut(s) 455, 646
BstKTI GATC 2 cut(s) 583, 599
BstMAI GTCTC 3 cut(s) 32, 237, 317
BstMBI GATC 2 cut(s) 580, 596
BstMWI GCNNNNNNNGC 6 cut(s) 64, 500, 546, 612, 632, 641
BstNI CCWGG 1 cut(s) 378
BstSCI CCNGG 1 cut(s) 376
BstSFI CTRYAG 1 cut(s) 171
BstUI CGCG 1 cut(s) 67
BstV1I GCAGC 3 cut(s) 196, 393, 490
BstV2I GAAGAC 1 cut(s) 360
Bsu15I ATCGAT 1 cut(s) 595
BsuTUI ATCGAT 1 cut(s) 595
BtsCI GGATG 2 cut(s) 140, 146
Cac8I GCNNGC 2 cut(s) 526, 613
CaiI CAGNNNCTG 1 cut(s) 589
CfoI GCGC 2 cut(s) 455, 646
Cfr10I RCCGGY 1 cut(s) 611
ClaI ATCGAT 1 cut(s) 595
CseI GACGC 4 cut(s) 56, 147, 495, 645
Csp6I GTAC 3 cut(s) 200, 515, 560
CviAII CATG 4 cut(s) 221, 434, 546, 577
CviQI GTAC 3 cut(s) 200, 515, 560
DdeI CTNAG 2 cut(s) 254, 297
DpnI GATC 2 cut(s) 582, 598
DpnII GATC 2 cut(s) 580, 596
Eam1104I CTCTTC 2 cut(s) 109, 196
EarI CTCTTC 2 cut(s) 109, 196
EciI GGCGGA 2 cut(s) 20, 70
Eco31I GGTCTC 1 cut(s) 237
EcoRII CCWGG 1 cut(s) 376
EcoT22I ATGCAT 1 cut(s) 551
FaeI CATG 4 cut(s) 224, 437, 549, 580
FatI CATG 4 cut(s) 220, 433, 545, 576
Fnu4HI GCNGC 4 cut(s) 20, 210, 382, 504
FokI GGATG 2 cut(s) 127, 133
Fsp4HI GCNGC 4 cut(s) 20, 210, 382, 504
FspBI CTAG 2 cut(s) 167, 525
GlaI GCGC 2 cut(s) 454, 645
GluI GCNGC 4 cut(s) 20, 210, 382, 504
GsuI CTGGAG 1 cut(s) 531
HaeII RGCGCY 2 cut(s) 456, 647
HapII CCGG 2 cut(s) 150, 612
HgaI GACGC 4 cut(s) 56, 147, 495, 645
HhaI GCGC 2 cut(s) 455, 646
Hin1I GRCGYC 1 cut(s) 656
Hin1II CATG 4 cut(s) 224, 437, 549, 580
Hin6I GCGC 2 cut(s) 453, 644
HinP1I GCGC 2 cut(s) 453, 644
HinfI GANTC 2 cut(s) 258, 592
HpaII CCGG 2 cut(s) 150, 612
HphI GGTGA 1 cut(s) 153
Hpy166II GTNNAC 2 cut(s) 112, 560
Hpy188I TCNGA 3 cut(s) 242, 347, 591
Hpy188III TCNNGA 4 cut(s) 150, 331, 423, 510
Hpy8I GTNNAC 2 cut(s) 112, 560
Hpy99I CGWCG 2 cut(s) 46, 661
HpyAV CCTTC 3 cut(s) 172, 242, 305
HpyCH4III ACNGT 2 cut(s) 53, 98
HpyCH4V TGCA 2 cut(s) 404, 549
HpyF10VI GCNNNNNNNGC 6 cut(s) 64, 500, 546, 612, 632, 641
HpyF3I CTNAG 2 cut(s) 254, 297
Hsp92I GRCGYC 1 cut(s) 656
Hsp92II CATG 4 cut(s) 224, 437, 549, 580
HspAI GCGC 2 cut(s) 453, 644
Kpn2I TCCGGA 1 cut(s) 149
KroI GCCGGC 1 cut(s) 611
KroNI GCCGGC 1 cut(s) 613
Kzo9I GATC 2 cut(s) 580, 596
LguI GCTCTTC 2 cut(s) 109, 196
LmnI GCTCC 2 cut(s) 314, 623
Lsp1109I GCAGC 3 cut(s) 196, 393, 490
LweI GCATC 1 cut(s) 475
MaeI CTAG 2 cut(s) 167, 525
MaeIII GTNAC 3 cut(s) 159, 223, 292
MalI GATC 2 cut(s) 582, 598
MboI GATC 2 cut(s) 580, 596
MboII GAAGA 5 cut(s) 66, 96, 183, 362, 365
MhlI GDGCHC 1 cut(s) 672
MluCI AATT 5 cut(s) 61, 182, 390, 428, 532
MnlI CCTC 6 cut(s) 88, 148, 236, 280, 458, 565
Mph1103I ATGCAT 1 cut(s) 551
MroI TCCGGA 1 cut(s) 149
MroNI GCCGGC 1 cut(s) 611
MslI CAYNNNNRTG 1 cut(s) 483
MspI CCGG 2 cut(s) 150, 612
MspR9I CCNGG 1 cut(s) 378
MvaI CCWGG 1 cut(s) 378
MvnI CGCG 1 cut(s) 67
MwoI GCNNNNNNNGC 6 cut(s) 64, 500, 546, 612, 632, 641
NaeI GCCGGC 1 cut(s) 613
NdeII GATC 2 cut(s) 580, 596
NgoMIV GCCGGC 1 cut(s) 611
NheI GCTAGC 1 cut(s) 524
NlaIII CATG 4 cut(s) 224, 437, 549, 580
NlaIV GGNNCC 1 cut(s) 375
NmuCI GTSAC 2 cut(s) 159, 292
NsiI ATGCAT 1 cut(s) 551
PciSI GCTCTTC 2 cut(s) 109, 196
PdiI GCCGGC 1 cut(s) 613
PfeI GAWTC 2 cut(s) 258, 592
PkrI GCNGC 4 cut(s) 21, 211, 383, 505
Psp6I CCWGG 1 cut(s) 376
PspGI CCWGG 1 cut(s) 376
PspN4I GGNNCC 1 cut(s) 375
PstNI CAGNNNCTG 1 cut(s) 589
RsaI GTAC 3 cut(s) 201, 516, 561
RsaNI GTAC 3 cut(s) 200, 515, 560
RseI CAYNNNNRTG 1 cut(s) 483
SapI GCTCTTC 2 cut(s) 109, 196
SatI GCNGC 4 cut(s) 20, 210, 382, 504
Sau3AI GATC 2 cut(s) 580, 596
ScaI AGTACT 1 cut(s) 516
ScrFI CCNGG 1 cut(s) 378
SduI GDGCHC 1 cut(s) 672
SetI ASST 9 cut(s) 16, 60, 172, 253, 277, 291, 386, 628, 678
SfaNI GCATC 1 cut(s) 475
SfcI CTRYAG 1 cut(s) 171
SmiMI CAYNNNNRTG 1 cut(s) 483
Sse9I AATT 5 cut(s) 61, 182, 390, 428, 532
SsiI CCGC 3 cut(s) 20, 31, 81
SspMI CTAG 2 cut(s) 167, 525
StyD4I CCNGG 1 cut(s) 376
TaaI ACNGT 2 cut(s) 53, 98
TaqI TCGA 2 cut(s) 595, 648
TasI AATT 5 cut(s) 61, 182, 390, 428, 532
TatI WGTACW 2 cut(s) 514, 559
TauI GCSGC 1 cut(s) 22
TfiI GAWTC 2 cut(s) 258, 592
TseFI GTSAC 2 cut(s) 159, 292
TseI GCWGC 3 cut(s) 209, 381, 503
Tsp45I GTSAC 2 cut(s) 159, 292
TspDTI ATGAA 3 cut(s) 17, 183, 422
XapI RAATTY 2 cut(s) 428, 532
XspI CTAG 2 cut(s) 167, 525
ZrmI AGTACT 1 cut(s) 516
Zsp2I ATGCAT 1 cut(s) 551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.